CN101878788A - Conopomorpha sinensis bradley and egg repellent - Google Patents
Conopomorpha sinensis bradley and egg repellent Download PDFInfo
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- CN101878788A CN101878788A CN2010102060494A CN201010206049A CN101878788A CN 101878788 A CN101878788 A CN 101878788A CN 2010102060494 A CN2010102060494 A CN 2010102060494A CN 201010206049 A CN201010206049 A CN 201010206049A CN 101878788 A CN101878788 A CN 101878788A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention discloses a conopomorpha sinensis bradley and egg repellent which comprises the main components of calamondin essential oil, clary sage essential oil, alpha-pinene, citronellal and other additives (sustained release agent, stabilizer and the like), wherein the essential oil and single substances belong to plant volatile secondary substances. The repellent has very strong repellent effect to conopomorpha sinensis bradley, is characterized by safety, high efficiency and no environmental pollution and can significantly reduce the harm of the conopomorpha sinensis bradley, achieve the purpose of effective prevention and treatment and be conductive to sustained high-efficient product of an orchard.
Description
Technical field:
The present invention relates to insect repellent, specifically, is a kind of lichee base of a fruit moth and worm's ovum repellant.
Background technology:
Lichee base of a fruit moth (Conopomorpha sinensis Bradley) also claims the line in the Eight Diagrams line thin moth, belongs to the Lepidoptera Gracilariidae, is the primary pest of lichee, longan.Mainly be distributed in places such as China, Thailand, Nepal and India.In China, this worm mainly is distributed in each lichee producing region such as Guangxi, Guangdong, Fujian and Taiwan.Lichee base of a fruit moth is a borer pest, and the female moth of this worm, originates in ovum on the pericarp between breeding time at lichee, longan fruit, and moth was gone into fruit inside after ovum was hatched into larva, caused a large amount of sheddings.Since the eighties in 20th century, cause harm lichee, longan of lichee base of a fruit moth presents more and more serious trend.General wormed fruit rate is 20%-40%, can reach more than 90% when high.Production loss is up to more than 50%, and has a strong impact on fruit.
Lichee base of a fruit moth is caused the reason that seriously causes harm: at first, directly pierce in the parasitic tissue after the larva hatching, traditional tagging property medicament is difficult to prove effective; Secondly, lichee base of a fruit moth generation time is short, the egg-laying period is longer, and generation overlap is serious, and chemical control often lacks specific aim; At last, the agriculture industrialization degree is not high, the orchard extensive management, and control is difficult to coordinate, and causes lichee base of a fruit moth food chain abundant, and the insect population radix is high.
Control lichee base of a fruit moth mainly is based on chemical control at present.But chemical control causes the residue of pesticide on the lichee too high, destroys environment, and insect is rampant again, problems such as decline of natural enemy quantity and bio-diversity reduction.So, seek low toxicity, low-residual, to person poultry safety, significant to harmonious friendly, the economic novel product of environment.
At present, had in a large number about plant secondary substance planting property of great majority food insect has been played the report of behavior regulation and control, made full use of these plant secondary substances, insect is carried out repellent or lures, reduced worm source radix, in the insect Ecological Control, will bring into play significantly acting on.
China's lichee area under cultivation has reached 71.67 ten thousand hm
2, only Guangdong Province has just surpassed 33.3 ten thousand hm
2But lichee base of a fruit moth is caused harm to lichee, longan and is becoming increasingly rampant, and has caused enormous economic loss, and has used chemical pesticide in a large number, causes serious, the environmental pollution of litchi fruits residue of pesticide.Lichee base of a fruit moth and worm's ovum repellant have safety, efficient characteristics, and environmentally safe is applicable to the nuisanceless or pollution-free food production of lichee, longan, has bright development prospect.
Summary of the invention:
The objective of the invention is to overcome the deficiency that prior art exists, seek the method for a kind of new control lichee base of a fruit moth, provide a kind of effectively, the lichee base of a fruit moth and the worm's ovum repellant of environmental protection, it comprises essential oil and single material of following percentage by weight:
Cinnamon oil 2%-5%
Citronella oil 3%-5%
Calamondin essential oil 8%-10%
Cinnamic acid 3%-5%
Citronellal 3%-5%
A-firpene 10%-15%
Emulsifier 7%-9%
Other auxiliary agents 3%-5%
Preferably, the essential oil and the single material that comprise following percentage by weight:
Cinnamon oil 3%-5%
Citronella oil 2%-5%
Calamondin essential oil 8%-10%
Cinnamic acid 2%-5%
Citronellal 3%-5%
A-firpene 12%-15%
Emulsifier 7%-9%
Other auxiliary agents 3%-5%
Wherein, emulsifier is: Tween 80, agricultural newborn 500#, agricultural newborn 600#;
Other auxiliary agents are: sustained release agent, stabilizing agent (aluminium-magnesium silicate SF-04, BHT, DHT).
Existing essential oil class material has 17 kinds of calamondin essential oils, sage clary oil, Rosemary Oil, citronella oil, orange oil, grapefruit oil, cedar oil, mugwort oil, abies oil, patchouli oil, tangerine oil, nutmeg essential oil, lavender oil, garlic oil, geranium oil, anise oil, cloves wet goods.
Existing single material has 17 kinds of australene, amphene, linalool, borneol, alpha-terpineol, laurene, citronellol, β-caryophyllene, camphor, nopinene, natural lemon aldehyde, nerol, terpinene, geranyl acetate, Cineole, citronellyl acetate, citronellals etc.
The present invention has selected rational essential oil and single material, and collaborative onset in lichee base of a fruit moth and worm's ovum repellent, has very obvious effects.
Compared with prior art, the present invention has following beneficial effect: use phytovolatilization secondary substance (comprising essential oil and single material) fully, " push away-La " strategy according to the new insect that disturbs based on the insect behavior both at home and abroad, the host's identification and host location of lichee base of a fruit moth of developing and worm's ovum repellant by disturbing lichee base of a fruit moth, behaviors such as mating and searching spawning place, control field lichee base of a fruit moth population quantity, has safety, efficient characteristics, environmentally safe, be applicable to lichee, nuisanceless or the pollution-free food production of longan has bright development prospect.
Description of drawings:
Fig. 1 is the gas chromatograph-mass spectrometer (GC-MS) constituent analysis figure of calamondin essential oil;
Fig. 2 is the gas chromatograph-mass spectrometer (GC-MS) constituent analysis figure of sage clary oil;
Fig. 3 is the gas chromatograph-mass spectrometer (GC-MS) constituent analysis figure of Rosemary Oil;
Fig. 4 is the gas chromatograph-mass spectrometer (GC-MS) constituent analysis figure of citronella oil.
Embodiment:
1.GC-MS analyze
Utilization GC-MS technical point is separated out the volatile ingredient to the best four kinds of essential oils (calamondin essential oil, sage clary oil, Rosemary Oil, citronella oil) of lichee base of a fruit moth repellent effect, replace natural plant essential oils list material with the single material of chemosynthesis, and interpolation auxiliary agent (sustained release agent, stabilizing agent etc.), as lichee base of a fruit moth and worm's ovum repellant, be applied to field control.Concrete main points are as follows:
Essential oil is directly ordered from company, and as Guangdong hundred fragrant material chemicals Co., Ltds etc., buying essential oil has calamondin essential oil, Rosemary Oil etc.Finnigan Trace GC-MS, and DB-1 (capillary column of 30m * 0.25mm), heating schedule: 50 ℃ of column temperatures, keep 1min, rise to 120 ℃ with 5 ℃/min, keep 5min, again with 10 ℃/min to 220 ℃; 230 ℃ of injection ports, carrier gas He, flow velocity 1.0ml/min, split sampling 15: 1.Ionization mode EI, 70ev, 250 ℃ of ion source temperatures, mass scanning scope 35～395amu.Each component is used the content that the chromatographic peak area normalization method is measured each composition through Willey and Mainlib standard spectrum library searching and analysis.
Be divided into and analysed calamondin essential oil, sage clary oil, Rosemary Oil, 4 kinds of derived essential oils of citronella oil.
By analysis, calamondin essential oil main component has 11 kinds: dipentene, repefral, squalene, diisooctyl phthalate, linalool, citral, (Z)-3,7-dimethyl-2,6-octadiene aldehyde, allyl cyclohexyl propionate, chloro-carbonic acid-2-Octyl Nitrite, phthalic monoester, dioctyl phthalate etc.The results are shown in Figure 1.
By analysis, the sage clary oil main component has 9 kinds: myrcene, 3,7-dimethyl-1,3,7-sarohornene, laurene, linalool, alpha-terpineol, neryl acetate, geranyl acetate, β-caryophyllene, germacrene etc.The results are shown in Figure 2.
By analysis, the rosemary oil main component has 8 kinds: nopinene, two isopropyl toluene, citrene, Cineole, terpinene, camphor, compounding acetic isoborneol ester, amphene etc.The results are shown in Figure 3.
By analysis, the citronella oil main component has 8 kinds: citronellal, citronellol, citronellyl acetate, camphoroxime, nerol, geraniol, australene, bergamio etc.The results are shown in Figure 4.
2. biochemical measurement
2.1 the lab screening of plants essential oil test
2.1.1 lichee base of a fruit moth there is the plants essential oil of repellent action with the electrophysiologic response screening
Tentaculum electric potential (Electroantennograms, abbreviation EAG) assay method is cut lichee base of a fruit moth whole piece feeler along base portion, after cutting off the feeler tip feeler is connected with 2 electrodes by electric silica gel, regulates the duty of instrument, treat to measure after baseline steadily.The stimulation air-flow is 40mL/min, and continuous flow is 50mL/min.
All essential oils are made into 5 μ l concentration, are that standard specimen carries out lichee base of a fruit moth tentaculum electric potential mensuration with 5 μ l citronella oils.Each sample repetitive stimulation 5 times, its mean value are the EAG absolute value of sample; The intact sample of every survey is promptly used the standard specimen repetitive stimulation 2 times, and its mean value is standard specimen EAG absolute value.Each stimulus intervals time is 30S.10 feelers of each sample test (5 female 5 heros).
The computational methods of tentaculum electric potential relative value be mean value with 2 standard specimens in front and back as 100, the average EAG absolute value x100 of 2 standard specimens in EAG absolute value/front and back of EAG relative value=sample analyzes the relatively potentiometric response difference between each extract sample.The size of EAG value is relevant to the size of the physiologically active of feeler with sample.
Obtain effect preferably essential oil be cinnamon oil and citronella oil, the EAG relative value is respectively 101.43 and 100.
2.1.2 lichee base of a fruit moth there is the plants essential oil of repellent action with the screening of four arm olfactometers
At essential oil 1ml dosage, under the 100ml/min throughput, measure the repellent action of different essential oils to lichee base of a fruit moth.
The repellent action power is in proper order: calamondin essential oil, cinnamon oil, citronella oil, sage clary oil, Rosemary Oil, orange oil, grapefruit oil, cedar oil, mugwort oil, abies oil, patchouli oil, tangerine oil, nutmeg essential oil, lavender oil, garlic oil, geranium oil, anise oil, caryophyllus oil, scattering ratio is respectively: 85.0%, 83.3%, 77.0%, 75.0%, 74.4%, 72.4%, 71.0%, 66.7%, 65.9%, 65.0%, 64.0%, 63.3%, 61.5%, 60.6%, 60.0%, 50.0%, 46.9%, 38.4%.
The essential oil of repellent effect more than 75% is calamondin essential oil, cinnamon oil, citronella oil and sage clary oil.
2.2 single material repellent action is measured
2.2.1 lichee base of a fruit moth is to the electrophysiologic response of single material
Effect is preferably cinnamic acid and citronellal, and the EAG relative value is 78.36 and 75.65.
2.2.2 four arm olfactometers of single material test
At single material 1ml dosage, under the 100ml/min throughput, different single materials are to the repellent action of lichee base of a fruit moth:
The repellent action power is in proper order: australene, citronellal, cinnamic acid, amphene, linalool, borneol, alpha-terpineol, laurene, citronellol, β-caryophyllene, camphor, nopinene, natural lemon aldehyde, nerol, terpinene, geranyl acetate, Cineole, citronellyl acetate, scattering ratio is respectively: 88.20%, 87.5%, 85.3%, 82.4%, 81.3%, 75.9%, 75.0%, 73.3%, 70.0%, 65.4%, 60.0%, 57.1%, 56.8%, 50.0%, 45.5%, 41.7%, 35.6%, 26.3%.
Scattering ratio has australene, cinnamic acid and citronellal at the material more than 85%.
The repellent effect laboratory test 2.3 lay eggs
Choose essential oil calamondin essential oil, sage clary oil, rosemary oil, citronella oil and geranium oil, and australene, amphene, linalool, alpha-terpineol, citronellal and terpinene carry out the repellency test of laying eggs of lichee base of a fruit moth.
Selectivity test method: dry the lichee fresh fruit after with the clear water wash clean standby naturally, with each test plant oil with acetone diluted to the 10mg/mL soup, 5 lichee fresh fruits of the same size are chosen in every processing, with miniaturised nebuliser soup evenly is sprayed on the fruit, control group spray clear water dries the back for examination.Fresh fruit after handling is put into the culture dish that is lined with filter paper, wherein handle and contrast each two culture dish, to handle and contrast intersection by diagonal is positioned in the dependent insect cage, insert 15 pairs 5 ages in days the mating adult in cage, and hang an absorbent cotton that has dipped in 5% honey at the cage inner top and supplement the nutrients for adult.Check behind the 48h on the fruit the ovum amount.Test repeats 3 times.
Scattering ratio=(check plot egg laying amount-treatment region the egg laying amount)/check plot egg laying amount of laying eggs.
The non-host plant secondary pollutant of repellent effect the best of obtaining laying eggs has calamondin essential oil, fragrant Fructus Perillae quintessence oil, australene, citronellal, and the selectivity scattering ratio of laying eggs reaches 91.6%, 85.2%, 92.5%, 86.3% respectively.
Active ingredient calamondin essential oil, fragrant Fructus Perillae quintessence oil, australene, citronellal, auxiliary agent.
Embodiment 1
1-4 further describes the present invention with reference to the accompanying drawings, selects the raw material of following weight ratio:
Calamondin essential oil 10%
Australene 14%
Dimethylbenzene 47%
Aluminium-magnesium silicate SF-04 4%
The preparation method is as follows: above-mentioned raw materials is added in a mixing bowl in proportion successively, and stirring at normal temperature mixed it in 2 hours, promptly made product.
Embodiment 2
A kind of lichee base of a fruit moth and worm's ovum repellant comprise the raw material of following weight ratio:
Calamondin essential oil 10%
Australene 13%
The newborn 500# 9% of farming
Methyl alcohol 47%
Aluminium-magnesium silicate BHT 5%
The preparation method is with example 1.
Embodiment 3
A kind of lichee base of a fruit moth and worm's ovum repellant comprise the raw material of following weight ratio:
Calamondin essential oil 10%
The newborn 600# 8% of farming
Dimethylbenzene 42%
Aluminium-magnesium silicate BHT 5%
The preparation method is with example 1.
Claims (8)
1. lichee base of a fruit moth and worm's ovum repellant is characterized in that, comprise following raw materials by weight percent:
Cinnamon oil 2%-5%
Citronella oil 3%-5%
Calamondin essential oil 8%-10%
Cinnamic acid 3%-5%
Citronellal 3%-5%
Australene 10%-15%
Emulsifier 7%-9%
Dimethylbenzene 40%-50% or methyl alcohol 40%-50%
Other auxiliary agents 3%-5%
Wherein, emulsifier is: any one among Tween 80, agricultural newborn 500#, the agricultural newborn 600#, other auxiliary agents are the wherein a kind of of sustained release agent, stabilizing agent, and described stabilizing agent is aluminium-magnesium silicate SF-04, BHT, DHT.
2. lichee base of a fruit moth according to claim 1 and worm's ovum repellant is characterized in that, comprise following raw materials by weight percent:
Cinnamon oil 2%-5%
Citronella oil 3%-5%
Calamondin essential oil 8%-10%
Cinnamic acid 3%-5%
Citronellal 3%-5%
Australene 10%-15%
Emulsifier 7%-9%
Dimethylbenzene 40%-50%
Other auxiliary agents 3%-5%
Wherein, emulsifier is: any one among Tween 80, agricultural newborn 500#, the agricultural newborn 600#, other auxiliary agents are the wherein a kind of of sustained release agent, stabilizing agent, and described stabilizing agent is aluminium-magnesium silicate SF-04, BHT, DHT.
3. lichee base of a fruit moth according to claim 1 and worm's ovum repellant is characterized in that, comprise following raw materials by weight percent:
Cinnamon oil 2%-5%
Citronella oil 3%-5%
Calamondin essential oil 8%-10%
Cinnamic acid 3%-5%
Citronellal 3%-5%
Australene 10%-15%
Emulsifier 7%-9%
Methyl alcohol 40%-50%
Other auxiliary agents 3%-5%
Wherein, emulsifier is: any one among Tween 80, agricultural newborn 500#, the agricultural newborn 600#, other auxiliary agents are the wherein a kind of of sustained release agent, stabilizing agent, and described stabilizing agent is aluminium-magnesium silicate SF-04, BHT, DHT.
4. lichee base of a fruit moth according to claim 1 and worm's ovum repellant is characterized in that, comprise following raw materials by weight percent:
Cinnamon oil 3%-5%
Citronella oil 2%-5%
Calamondin essential oil 8%-10%
Cinnamic acid 2%-5%
Citronellal 3%-5%
Australene 12%-15%
Emulsifier 7%-9%
Dimethylbenzene 40%-45% or methyl alcohol 40%-45%
Other auxiliary agents 3%-5%
Wherein, emulsifier is: any one among Tween 80, agricultural newborn 500#, the agricultural newborn 600#, other auxiliary agents are the wherein a kind of of sustained release agent, stabilizing agent, and described stabilizing agent is aluminium-magnesium silicate SF-04, BHT, DHT.
5. lichee base of a fruit moth according to claim 4 and worm's ovum repellant is characterized in that, comprise following raw materials by weight percent:
Cinnamon oil 3%-5%
Citronella oil 2%-5%
Calamondin essential oil 8%-10%
Cinnamic acid 2%-5%
Citronellal 3%-5%
Australene 12%-15%
Emulsifier 7%-9%
Dimethylbenzene 40%-45%
Other auxiliary agents 3%-5%
Wherein, emulsifier is: any one among Tween 80, agricultural newborn 500#, the agricultural newborn 600#, other auxiliary agents are the wherein a kind of of sustained release agent, stabilizing agent, and described stabilizing agent is aluminium-magnesium silicate SF-04, BHT, DHT.
6. lichee base of a fruit moth according to claim 4 and worm's ovum repellant is characterized in that, comprise following raw materials by weight percent:
Cinnamon oil 3%-5%
Citronella oil 2%-5%
Calamondin essential oil 8%-10%
Cinnamic acid 2%-5%
Citronellal 3%-5%
Australene 12%-15%
Emulsifier 7%-9%
Methyl alcohol 40%-45%
Other auxiliary agents 3%-5%
Wherein, emulsifier is: any one among Tween 80, agricultural newborn 500#, the agricultural newborn 600#, other auxiliary agents are the wherein a kind of of sustained release agent, stabilizing agent, and described stabilizing agent is aluminium-magnesium silicate SF-04, BHT, DHT.
7. lichee base of a fruit moth according to claim 1 and worm's ovum repellant is characterized in that, comprise following raw materials by weight percent:
Cinnamon oil 4%
Citronella oil 5%
Calamondin essential oil 10%
Cinnamic acid 5%
Citronellal 5%
Australene 15%
Tween 80 7%
Dimethylbenzene 45%
Aluminium-magnesium silicate SF-04 5%.
8. lichee base of a fruit moth according to claim 1 and worm's ovum repellant is characterized in that, comprise following raw materials by weight percent:
Cinnamon oil 4%
Citronella oil 4%
Calamondin essential oil 9%
Cinnamic acid 5%
Citronellal 5%
Australene 14%
The newborn 500# 9% of farming
Methyl alcohol 45%
Aluminium-magnesium silicate BHT 5%.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102326542A (en) * | 2011-10-24 | 2012-01-25 | 中国农业大学 | New use of palmitic acid as meloidogyne incognita repellent |
| CN102696597A (en) * | 2012-06-29 | 2012-10-03 | 漳州市英格尔农业科技有限公司 | Artificially synthesized sex pheromone and lure of conopomorpha sinensis |
| CN104807941A (en) * | 2015-05-13 | 2015-07-29 | 吉林烟草工业有限责任公司 | Detection method of terpene alcohol ester compounds in edible flavor and fragrance |
| CN109874810A (en) * | 2019-01-30 | 2019-06-14 | 广东省农业科学院植物保护研究所 | A kind of Conopomorpha sinensis Bradley adult stage cooperates with preventing control method with the ovum phase |
| CN110326614A (en) * | 2019-07-24 | 2019-10-15 | 广东省生物资源应用研究所 | A kind of Conopomorpha sinensis Bradley botanical attractant and its control method |
| CN116326547A (en) * | 2023-01-06 | 2023-06-27 | 广东省农业科学院植物保护研究所 | Method for determining indoor toxicity of larvae of litchi pedicel borers |
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2010
- 2010-06-09 CN CN2010102060494A patent/CN101878788A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102326542A (en) * | 2011-10-24 | 2012-01-25 | 中国农业大学 | New use of palmitic acid as meloidogyne incognita repellent |
| CN102696597A (en) * | 2012-06-29 | 2012-10-03 | 漳州市英格尔农业科技有限公司 | Artificially synthesized sex pheromone and lure of conopomorpha sinensis |
| CN104807941A (en) * | 2015-05-13 | 2015-07-29 | 吉林烟草工业有限责任公司 | Detection method of terpene alcohol ester compounds in edible flavor and fragrance |
| CN109874810A (en) * | 2019-01-30 | 2019-06-14 | 广东省农业科学院植物保护研究所 | A kind of Conopomorpha sinensis Bradley adult stage cooperates with preventing control method with the ovum phase |
| CN109874810B (en) * | 2019-01-30 | 2021-01-05 | 广东省农业科学院植物保护研究所 | Litchi fruit borer adult stage and egg stage cooperative prevention and control method |
| CN110326614A (en) * | 2019-07-24 | 2019-10-15 | 广东省生物资源应用研究所 | A kind of Conopomorpha sinensis Bradley botanical attractant and its control method |
| CN110326614B (en) * | 2019-07-24 | 2021-06-29 | 广东省科学院动物研究所 | Litchi fruit borer plant source attractant and control method thereof |
| CN116326547A (en) * | 2023-01-06 | 2023-06-27 | 广东省农业科学院植物保护研究所 | Method for determining indoor toxicity of larvae of litchi pedicel borers |
| CN116326547B (en) * | 2023-01-06 | 2023-11-10 | 广东省农业科学院植物保护研究所 | Indoor toxicity determination method for larvae of litchi pedicel borers |
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Application publication date: 20101110 |