CN107736387A - A kind of biological control method of longicorn beetle - Google Patents
A kind of biological control method of longicorn beetle Download PDFInfo
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- CN107736387A CN107736387A CN201711263887.3A CN201711263887A CN107736387A CN 107736387 A CN107736387 A CN 107736387A CN 201711263887 A CN201711263887 A CN 201711263887A CN 107736387 A CN107736387 A CN 107736387A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/02—Acyclic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/26—Meliaceae [Chinaberry or Mahogany family], e.g. mahogany, langsat or neem
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/32—Ranunculaceae [Buttercup family], e.g. hepatica, hydrastis or goldenseal
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Abstract
The present invention provides a kind of biological control method of longicorn beetle, and described method is that the control agent of different formulations is used in longicorn beetle growth different phase, with strong points；Bacillus thuringiensis zymotic fluid, adonis are soaked juice and absolute ethyl alcohol and are used cooperatively by the longicorn beetle ovum phase, and the kill rate of longicorn beetle ovum is up to more than 100%；Bacillus thuringiensis zymotic fluid is soaked juice by larval phase with melia toosendan skin, absolute ethyl alcohol is used cooperatively, the kill rate of longicorn beetle larva is up to more than 97%, bacillus thuringiensis zymotic fluid, hydrogen peroxide, absolute ethyl alcohol and adonis leaching juice are used cooperatively by the adult stage, the kill rate of longicorn beetle adult is up to more than 98%, effectively preventing and treating longicorn beetle quantity, avoid the application using cytotoxic chemical insecticides such as zinc phosphide, aluminum phosphate, reduce environmental pollution, the main bacillus thuringiensis zymotic fluid for using biological source, to people and animals and fanout free region, belong to the very high Biocidal source of security.
Description
Technical field
The invention belongs to technical field of biological control, and in particular to a kind of biological control method of longicorn beetle.
Background technology
A variety of trees such as longicorn beetle main harm method paulownia, apple, pears, poplar, elm, willow, but be harm in urban green-making tree
The important trunk borer of method tung oil tree.Because method tung oil tree growth potential is strong, tree crown is big, there is the features such as stronger adsorption capacity to pollutant,
It is one of important Landscape Trees in China.The harm of longicorn beetle in recent years and generation are increasingly severe, general in many places
Cause disaster excessively, cause mortality or undergrowth frequency to die on one's deathbed, brought about great losses to Ecological environment and urban afforestation, it has also become
Urban forestry, afforestation, the Severe pests of Construction of Eco-urban development.
At present for the study on prevention of longicorn beetle class trunk borer it is more be chemical prevention, chemical prevention is to produce at present
The upper relatively broad and direct prevention and controls of application.Mainly include：Inserted at larval phase defecation hole the stronger zinc phosphide of volatility,
Aluminum phosphate poison label, DDT EC etc. are eaten with reducing larva to the moth of trunk, and adult is killed in adult stage cover spray；Ovum phase medicine
Agent applies dry etc..But longicorn beetle, because the history of life is longer, its ovum, larva and pupa are all lived in trunk, individual is scattered, seeks solitary life
Living, being little affected by extraneous bad condition influences, so prevention effect is not highly desirable.Meanwhile method tung oil tree is typically used as gardens or city
City's green tree species, the use of a large amount of chemical pesticides can undoubtedly give ecology and human settlement to pollute and lack sustainability,
The purpose for improving environment with people's cultivation tung oil tree is runed counter to.Therefore, chemicals should be avoided as far as possible in actual applications
Especially there is the administration of volatile medicine.To seek effectively preventing method, the integrated control star day based on biological control measure
Ox is the direction given priority to from now on.
The content of the invention
In summary, in order to overcome the shortcomings of prior art problem, a kind of biological control method of longicorn beetle of the invention,
The main bacillus thuringiensis zymotic fluid for using biological source, is avoided using cytotoxic chemical insecticides such as zinc phosphide, aluminum phosphate
Using reducing environmental pollution, preserve the ecological environment.
In order to solve the above technical problems, the technical solution adopted by the present invention is：
A kind of biological control method of longicorn beetle, described biological control method comprise the following steps：
S1, determine growth conditions of the longicorn beetle on trees：The growth conditions include ovum phase, larval phase and adult stage；
S2, longicorn beetle ovum phase control agent is applied in the ovum phase, longicorn beetle larva control agent is applied in larval phase, star is applied in the adult stage
Cerambycid beetles control agent, complete the preventing and treating to longicorn beetle；
S3, described longicorn beetle ovum phase control agent, longicorn beetle larva control agent, longicorn beetle adult controlling agent dilute 200-500
Used after times；
S4, by longicorn beetle ovum phase control agent along the injection of ovulation hole, or longicorn beetle ovum phase control agent is applied on cotton balls and injected
Brushed in ovulation hole or on trees；
S5, described larval phase is that longicorn beetle larva control agent is injected into defecation hole, or longicorn beetle larva control agent is applied
It is put on cotton balls and injects in defecation hole or brushed on trees；
S6, described longicorn beetle adult controlling agent are to use to spray either tree injection method or brush on trees, and adult is invaded
When eating trees, play and good anti-kill effect.
Further, the biological control method of described a kind of longicorn beetle, wherein, described longicorn beetle ovum phase control agent by with
The raw material of lower parts by weight is made：50 parts of bacillus thuringiensis zymotic fluid, 20 parts of juice of adonis leaching, 1.5 parts of absolute ethyl alcohol and breast
2 parts of agent.
Further, a kind of biological control method of longicorn beetle, wherein, described longicorn beetle larva control agent is by following weight
The raw material of part is made：80 parts of bacillus thuringiensis zymotic fluid, 10 parts of juice of melia toosendan skin leaching, 1.5 parts of absolute ethyl alcohol and emulsifying agent 1
Part.
Further, the biological control method of described a kind of longicorn beetle, wherein, described longicorn beetle adult controlling agent by with
The raw material of lower parts by weight is made：150 parts of bacillus thuringiensis zymotic fluid, hydrogen peroxide 3-5 parts of volume fraction 5%, absolute ethyl alcohol
5 parts and adonis 20 parts of juice of leaching.
Further, the biological control method of described a kind of longicorn beetle, wherein, described bacillus thuringiensis zymotic fluid
Be by after bacillus thuringiensis actication of culture, included fish meal, dregs of beans, starch and water zymotic fluid substrate fermentation after, collect
Supernatant liquid, then 800r/min centrifugations, collect what supernatant obtained.
Further, the biological control method of described a kind of longicorn beetle, wherein, described adonis leaching juice is to take fresh good fortune
Longevity grass blade and leaf, are smashed to pieces, are added sodium chloride, are ground into slurry, are then added water immersion, filtering, are taken its juice, are obtained after adding water constant volume
Arrive.
Further, the biological control method of described a kind of longicorn beetle, wherein, described melia toosendan skin leaching juice is to take toosendanin
Skin and root skin, water, sodium chloride, volume fraction 60-70% sulfuric acid are added, boils 30min, filtered, take its filtrate, add water constant volume
Obtain afterwards.
Further, the biological control method of described a kind of longicorn beetle, wherein, described emulsifying agent is polysorbas20, glycerine
Or any one of sorbester p17.
Further, the biological control method of described a kind of longicorn beetle, wherein, described bacillus thuringiensis zymotic fluid
It is prepared as follows：
（1）Bacillus thuringiensis strain is activated, and prepares bacillus thuringiensis seed liquor；
（2）Fish meal, dregs of beans, starch and water are well mixed according to 1: 2: 3: 85 mass ratio, obtain zymotic fluid matrix；
（3）Bacillus thuringiensis seed liquor is inoculated with into zymotic fluid matrix, wherein every kilogram of zymotic fluid matrix inoculation Su Yunjin
Bacillus seed liquor 100-200ml, then under the conditions of 28-32 DEG C, speed of agitator 220r/min, fermented and cultured 35-48h
Afterwards, 20-30min is stood, collects supernatant liquid, then 800r/min is centrifuged, and the supernatant of collection is sent out for bacillus thuringiensis
Zymotic fluid.
Compared with prior art, it is of the invention to have the advantages that：
The selectivity for the bacillus thuringiensis that the present invention selects is stronger, to people and animals and fanout free region, belongs to the very high life of security
Thing desinsection source, the control agent of different formulations is used in longicorn beetle growth different phase, it is with strong points；The longicorn beetle ovum phase is by Su Yunjin
Fermentation of bacillus liquid, adonis leaching juice and absolute ethyl alcohol are used cooperatively, and the kill rate of longicorn beetle ovum is up to more than 100%；Larva
Bacillus thuringiensis zymotic fluid is soaked juice by the phase with melia toosendan skin, absolute ethyl alcohol is used cooperatively, and the kill rate of longicorn beetle larva is reachable
More than 97%, bacillus thuringiensis zymotic fluid, hydrogen peroxide, absolute ethyl alcohol and adonis leaching juice are used cooperatively by the adult stage, star day
The kill rate of ox adult is effectively prevented and treated longicorn beetle quantity, avoided using cytotoxic chemicals such as zinc phosphide, aluminum phosphate up to more than 98%
The application of insecticide, reduces environmental pollution, and preserves the ecological environment.
Embodiment
The present invention is described in further detail with reference to embodiments.
The bacillus thuringiensis used in following embodiments is bacillus thuringiensis 8010（Preserving number
CGMCC1.1767）Microbial bacteria is purchased from China General Microbiological culture presevation administrative center, wherein, bacillus thuringiensis
8010 with Authorization Notice No. be 101988043B, entitled《Bacillus thuringiensis microbial insecticide and preparation method thereof with
Special culture media》The bacillus thuringiensis 8010 of record is identical bacterial strain；The materials such as fish meal, dregs of beans, starch are commercially available.
Embodiment 1：
A kind of biological control method of longicorn beetle comprises the following steps：
S1, determine growth conditions of the longicorn beetle on trees：The growth conditions include ovum phase, larval phase and adult stage；
S2, longicorn beetle ovum phase control agent is applied in the ovum phase, longicorn beetle larva control agent is applied in larval phase, star is applied in the adult stage
Cerambycid beetles control agent, complete the preventing and treating to longicorn beetle；
Described ovum phase control agent is made up of the component of following parts by weight：50 parts of bacillus thuringiensis zymotic fluid, adonis leaching
20 parts of juice, 1.5 parts of absolute ethyl alcohol, 2 parts of emulsifying agent；
Described longicorn beetle larva control agent is made up of the component of following parts by weight：80 parts of bacillus thuringiensis zymotic fluid, river
10 parts of juice of chinaberry skin leaching, 1.5 parts of absolute ethyl alcohol, 1 part of emulsifying agent；
Described longicorn beetle adult controlling agent is made up of the component of following parts by weight：150 parts of bacillus thuringiensis zymotic fluid, body
Hydrogen peroxide 3-5 parts of fraction 5%, 5 parts of absolute ethyl alcohol, adonis 20 parts of juice of leaching；
Described bacillus thuringiensis zymotic fluid is included fish meal, dregs of beans, shallow lake by after bacillus thuringiensis actication of culture
After the zymotic fluid substrate fermentation of powder and water, supernatant liquid is collected, then 800r/min is centrifuged, and collects what supernatant obtained；
Described bacillus thuringiensis zymotic fluid is prepared as follows：
（1）Bacillus thuringiensis strain is activated, and prepares bacillus thuringiensis seed liquor；
（2）Fish meal, dregs of beans, starch and water are well mixed according to 1: 2: 3: 85 mass ratio, obtain zymotic fluid matrix；
（3）Bacillus thuringiensis seed liquor is inoculated with into zymotic fluid matrix, wherein every kilogram of zymotic fluid matrix inoculation Su Yunjin
Bacillus seed liquor 100-200ml, then under the conditions of 28-32 DEG C, speed of agitator 220r/min, fermented and cultured 35-48h
Afterwards, 20-30min is stood, collects supernatant liquid, then 800r/min is centrifuged, and the supernatant of collection is sent out for bacillus thuringiensis
Zymotic fluid；
Described preparation 1L adonis leaching juice is to take fresh Fu Shou grass blade and leaf 200g, smashs to pieces, adds 1g sodium chloride, be ground into
Slurry, 500ml water immersion 1-1.5h is then added, filtering, its juice is taken, adds water to supply what 1L was obtained；
Described preparation 1L melia toosendans skin leaching juice is to take melia toosendan bark and root skin 200g, addition 500ml water, 5g sodium chloride, addition water,
The sulfuric acid of sodium chloride, 1ml volume fractions 60-70%, boils 30min, filtering, takes its filtrate, adds water to supply what 1L was obtained；
Described emulsifying agent is any one of polysorbas20, glycerine or sorbester p17；
S3, described longicorn beetle ovum phase control agent, longicorn beetle larva control agent, longicorn beetle adult controlling agent dilute 200-500
Used after times；
S4, by longicorn beetle ovum phase control agent along the injection of ovulation hole, or longicorn beetle ovum phase control agent is applied on cotton balls and injected
Brushed in ovulation hole or on trees；
S5, described larval phase is that longicorn beetle larva control agent is injected into defecation hole, or longicorn beetle larva control agent is applied
It is put on cotton balls and injects in defecation hole or brushed on trees；
S6, described longicorn beetle adult controlling agent are to use to spray either tree injection method or brush on trees, and adult is invaded
When eating trees, play and good anti-kill effect.
We provide some data below, the prevention effect with the explanation present invention to longicorn beetle.
First, the ovicidal efficiency of longicorn beetle ovum phase control agent：
It is utilized respectively embodiment 1 and selects 4 groups, 200 times of dilution agent is prevented and treated to the longicorn beetle ovum phase, then longicorn beetle ovum is carried out preventing killing,
Meanwhile with 80% DDT EC of equating volume as a control group, observe larvae hatch phenomenon, the results showed that, through 1-4 groups with
And control group is 100% without larvae hatch, longicorn beetle ovum kill rate, illustrating the longicorn beetle ovum phase control agent of the present invention can replace
In generation, has the DDT EC of chemical toxicity, reduces environmental pollution.
2nd, the larva kill rate of longicorn beetle larva control agent：
Longicorn beetle larva is collected, embodiment 1 is utilized respectively and selects 4 groups, 500 times of dilution agent is prevented and treated to longicorn beetle larva, then to star
Roundheaded borer carries out preventing killing, meanwhile, to wait the zinc phosphide of homogenous quantities as a control group, every group 50, observe and record larvae alive
Rate, the results showed that, the kill rate of the longicorn beetle larva through 1-4 groups is up to more than 97%, and the kill rate of control group longicorn beetle larva
Only 95% or so, in addition, the longicorn beetle larva control agent of 1-4 groups shortens 10-15%, explanation than the larva time of killing of zinc phosphide
The longicorn beetle larva control agent of the present invention can substitute the zinc phosphide with chemical toxicity, in addition than zinc phosphide prevention effect more
It is good, reduce environmental pollution.
3rd, the larva kill rate of longicorn beetle adult controlling agent
Longicorn beetle adult is collected, embodiment 1 is utilized respectively and selects 4 groups, to 400 times of longicorn beetle adult controlling dilution agent, then to star
Cerambycid beetles carry out preventing killing, meanwhile, to wait 56% phosphatization aluminium flake of homogenous quantities as a control group, every group 50, observe and record adult
Survival rate, the results showed that, the kill rate of the longicorn beetle adult through 1-4 groups is up to more than 98%, and control group longicorn beetle larva kills
Dead rate is only 96% or so, in addition, 1-4 longicorn beetle adult controlling agent shortens 5-10% than the adult time of killing of aluminum phosphate, is said
Bright longicorn beetle larva control agent of the invention can substitute the aluminum phosphate with chemical toxicity, or even the prevention effect than aluminum phosphate
More preferably, reduce environmental pollution.
Claims (9)
1. a kind of biological control method of longicorn beetle, it is characterised in that described biological control method comprises the following steps：
S1, determine growth conditions of the longicorn beetle on trees：The growth conditions include ovum phase, larval phase and adult stage；
S2, longicorn beetle ovum phase control agent is applied in the ovum phase, longicorn beetle larva control agent is applied in larval phase, star is applied in the adult stage
Cerambycid beetles control agent, complete the preventing and treating to longicorn beetle；
S3, described longicorn beetle ovum phase control agent, longicorn beetle larva control agent, longicorn beetle adult controlling agent dilute 200-500
Used after times；
S4, by longicorn beetle ovum phase control agent along the injection of ovulation hole, or longicorn beetle ovum phase control agent is applied on cotton balls and injected
Brushed in ovulation hole or on trees；
S5, described larval phase is that longicorn beetle larva control agent is injected into defecation hole, or longicorn beetle larva control agent is applied
It is put on cotton balls and injects in defecation hole or brushed on trees；
S6, described longicorn beetle adult controlling agent are to use to spray either tree injection method or brush on trees, and adult is invaded
When eating trees, play and good anti-kill effect.
A kind of 2. biological control method of longicorn beetle according to claim 1, it is characterised in that described longicorn beetle ovum phase
Control agent is made up of the raw material of following parts by weight：50 parts of bacillus thuringiensis zymotic fluid, 20 parts of juice of adonis leaching, absolute ethyl alcohol
1.5 parts and 2 parts of emulsifying agent.
3. according to a kind of biological control method of longicorn beetle of claim 1, it is characterised in that described longicorn beetle larva control agent
It is made up of the raw material of following parts by weight：80 parts of bacillus thuringiensis zymotic fluid, 10 parts of juice of melia toosendan skin leaching, 1.5 parts of absolute ethyl alcohol
With 1 part of emulsifying agent.
A kind of 4. biological control method of longicorn beetle according to claim 1, it is characterised in that described longicorn beetle adult
Control agent is made up of the raw material of following parts by weight：The hydrogen peroxide 3-5 of 150 parts of bacillus thuringiensis zymotic fluid, volume fraction 5%
Part, 5 parts of absolute ethyl alcohol and adonis 20 parts of juice of leaching.
A kind of 5. biological control method of longicorn beetle according to claim 1 or 4, it is characterised in that described Su Yunjin
Fermentation of bacillus liquid is included fish meal, dregs of beans, the fermentation liquid-based of starch and water by after bacillus thuringiensis actication of culture
After matter fermentation, supernatant liquid is collected, then 800r/min is centrifuged, and collects what supernatant obtained.
A kind of 6. biological control method of longicorn beetle according to claim 1 or 2, it is characterised in that described adonis
It is to take fresh Fu Shou grass blade and leaf to soak juice, is smashed to pieces, adds sodium chloride, is ground into slurry, then adds water immersion, filtering, takes its juice
Liquid, add what is obtained after water constant volume.
A kind of 7. biological control method of longicorn beetle according to claim 1 or 3, it is characterised in that described melia toosendan skin
It is to take melia toosendan bark and root skin to soak juice, adds water, sodium chloride, volume fraction 60-70% sulfuric acid, boils 30min, filters, takes it
Filtrate, add what is obtained after water constant volume.
8. the biological control method of a kind of longicorn beetle according to claim 1, it is characterised in that described emulsifying agent is to tell
Any one of temperature 20, glycerine or sorbester p17.
A kind of 9. biological control method of longicorn beetle according to claim 1 or 5, it is characterised in that described Su Yunjin
What fermentation of bacillus liquid was prepared as follows：
（1）Bacillus thuringiensis strain is activated, and prepares bacillus thuringiensis seed liquor；
（2）Fish meal, dregs of beans, starch and water are well mixed according to 1: 2: 3: 85 mass ratio, obtain zymotic fluid matrix；
（3）Bacillus thuringiensis seed liquor is inoculated with into zymotic fluid matrix, wherein every kilogram of zymotic fluid matrix inoculation Su Yunjin
Bacillus seed liquor 100-200ml, then under the conditions of 28-32 DEG C, speed of agitator 220r/min, fermented and cultured 35-48h
Afterwards, 20-30min is stood, collects supernatant liquid, then 800r/min is centrifuged, and the supernatant of collection is sent out for bacillus thuringiensis
Zymotic fluid.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109362791A (en) * | 2018-12-07 | 2019-02-22 | 珠海德源现代农业科技有限公司 | A kind of biological insecticides and preparation method thereof for preventing and treating longicorn insect pest |
| CN109874810A (en) * | 2019-01-30 | 2019-06-14 | 广东省农业科学院植物保护研究所 | A kind of Conopomorpha sinensis Bradley adult stage cooperates with preventing control method with the ovum phase |
| CN111820052A (en) * | 2019-04-23 | 2020-10-27 | 柳跃年 | Prevention and control method for blueberries anoplophora chinensis |
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| CN107155735A (en) * | 2017-05-18 | 2017-09-15 | 何振贤 | A kind of trees longicorn biological control method |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109362791A (en) * | 2018-12-07 | 2019-02-22 | 珠海德源现代农业科技有限公司 | A kind of biological insecticides and preparation method thereof for preventing and treating longicorn insect pest |
| CN109874810A (en) * | 2019-01-30 | 2019-06-14 | 广东省农业科学院植物保护研究所 | A kind of Conopomorpha sinensis Bradley adult stage cooperates with preventing control method with the ovum phase |
| CN109874810B (en) * | 2019-01-30 | 2021-01-05 | 广东省农业科学院植物保护研究所 | Litchi fruit borer adult stage and egg stage cooperative prevention and control method |
| CN111820052A (en) * | 2019-04-23 | 2020-10-27 | 柳跃年 | Prevention and control method for blueberries anoplophora chinensis |
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