CN108902446A - A kind of high protein lees feeds and preparation method thereof - Google Patents
A kind of high protein lees feeds and preparation method thereof Download PDFInfo
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- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A23K10/38—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material from distillers' or brewers' waste
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Abstract
The invention discloses a kind of high protein lees feeds and preparation method thereof.The preparation method of the high protein lees feeds is using distillers ' grains, dried silkworm chrysalis meal as major ingredient, step fermentation is carried out to vinasse using multi-cultur es such as Phanerochaete chrysosporium, trichoderma reesei and aspergillus nigers, depth degradation successively is carried out to lignin, cellulose and the hemicellulose in vinasse.The present invention has carried out effective control to the factors such as the selection of carbon source and nitrogen source in microbial fermentation degradation process and proportion, oxygen-supply quantity, intermediates, zymoexciter and inhibition inhibitor, improves the Feeding Value of coarse-fibred degradation rate, the utilization rate of vinasse and lees feeds；The crude fiber contents of the high protein lees feeds as made from fermentation process of the invention reduces by 12~15%, and crude protein content increases by 25~28%, and nutrition is closer to complete feed.
Description
Technical field
The present invention relates to the technical fields of animal feed, and in particular to a kind of high protein lees feeds and preparation method thereof.
Background technique
Distillers ' grains are the single raw materials such as sorghum, corn, wheat or mixed raw material is fermented and the residue after distilling,
It is feed raw material with high nutritive value.
Carbohydrate in brewed spirit raw material is fermented to separate after ethyl alcohol through distillation, the crude protein in distillers ' grains
Matter, crude fat equal size increase compared to raw material, and nitrogen-free extracts content then accordingly reduces, B family vitamin content also compared with
Height can be used as roughage and be added in the daily ration of poultry, but also contain a large amount of crude fibres in distillers ' grains, mostly come from white wine wine
The rice husk used during making as filler, these crude fibres include lignin, cellulose, hemicellulose, are unable to coverlet
Stomach animal is digested, and palatability is poor, can only be used to feeding ruminants.
For the feeding value for improving distillers ' grains, the prior art usually screens out the rice husk centrifugation in distillers ' grains, to remove it
Lignocellulose degradation in distillers ' grains is either fiber two using microbe fermentation method or enzymatic isolation method by middle major part crude fibre
The small molecular sugar that the nonruminants such as sugar, glucose can digest, while the part crude protein in distillers ' grains being made to be degraded to small-molecular peptides,
The nutritive value of distillers ' grains is improved to a certain extent.
Existing microbe fermentation method and enzymatic isolation method still have some shortcomings：Firstly, lignin and cellulose and half fiber
The mechanism of degradation for tieing up element is different, and microorganism has very strong specific aim to coarse-fibred degradation, and can only effectively degrading for having is wooden
Element, what is had effectively degraded cellulose and hemicellulose, selection one quasi-microorganism of any of them can only be used for crude fibre of degrading, all
Higher lignocellulose degradation rate cannot be obtained, for example, if only the trichoderma of energy degraded cellulose and hemicellulose is selected to ferment,
Then the lignin in rice husk cannot effectively be degraded, and lignin is bonding with cellulose and hemicellulose, and then to cellulose
Degradation with hemicellulose produces inhibiting effect, keeps crude fibre entirety degradation rate low；Secondly, not degrading to microbial fermentation
Some effects factor in the process is effectively controlled, such as carbon source and the selection and proportion of nitrogen source, intermediates and bottom
Object metal ion and its concentration；Finally, containing only vegetable protein with distillers ' grains fermentation high protein feed obtained, lack animal
Property albumen, nutrition is not comprehensive enough, can only be used as roughage, feed poultry when must cooperate other nutrient fodders.
Summary of the invention
In order to overcome the above deficiency of existing high protein lees feeds, the present invention provides one kind with distillers ' grains, dried silkworm chrysalis meal and is
High protein lees feeds of major ingredient and preparation method thereof.The high protein lees feeds utilize Phanerochaete chrysosporium, Richter scale wood
The multi-cultur es such as mould and aspergillus niger carry out step fermentation to vinasse, successively to lignin, cellulose and the hemicellulose in vinasse into
Row depth degradation improves the Feeding Value of coarse-fibred degradation rate, the utilization rate of vinasse and lees feeds；By hair of the invention
The crude fiber content of high protein lees feeds made from fermenting process reduces by 12~15%, and crude protein content increases by 25~28%, nutrition
More comprehensively.
Technical effect of the invention is achieved through the following technical solutions：
A kind of preparation method of high protein lees feeds, includes the following steps：
（1）Pretreatment, by fresh distillers ' grains 150~180 DEG C at a temperature of heat, obtain moisture content be 20%~24%
Fermentation raw material, to the fermentation raw material addition wheat bran after the drying as carbon source, the weight of the fermentation raw material and wheat bran is matched
Than being 5：1~8：1, ammonium salt, which is added, as quick-acting nitrogen sources and weight into the fermentation raw material is equivalent to fresh distillers ' grains weight
10~14% dried silkworm chrysalis meal is 6.0~7.0 as effect nitrogen source late, adjustment pH, and temperature is 28~32 DEG C, humidity is 82~88%, is obtained
To vinasse fermentation culture medium, the fermentation medium is subjected to sterilization treatment；
（2）Level-one degradation, the first strain is inoculated into the fermentation medium after the sterilizing, and first strain is yellow archespore
The flat lead fungi of hair, inoculum concentration are 5~8%, and level-one degradation feed is obtained after the 4~6d that ferments；
（3）Second level degradation, the temperature for adjusting level-one degradation feed is 26~30 DEG C, PH is 5.0~6.5, humidity be 85~
90%, the second strain is accessed, i.e., proportion is 2：1~4：1 trichoderma reesei and aspergillus niger, ferment 3~5d after obtain second level degradation raise
Material is neutralized, up to high protein lees feeds after drying after second level degradation feed inactivation with limewash.
Dried silkworm chrysalis meal is a kind of high protein, high-fat, amino acid comprehensively and the feed rich in macroelement and microelement adds
Add agent.Its methionine, lysine, tryptophane are high, are rich in leucine, isoleucine and B family vitamin, are balanced ration ammonia
The excellent component of base acid composition, is suitble to and other feed compatibilities；Up to 22% or more, metabolic energy level is up to its crude fat
11.71MJ/kg；Its gross protein value is up to 54%, can be used as the slow effect nitrogen source during vinasse fermentation；By quick-acting nitrogen source nitre
Hydrochlorate and the late combination of effect nitrogen source dried silkworm chrysalis meal can provide sufficient nitrogen source for the whole process of microorganism growth, breeding, promote white wine
Coarse-fibred degradation in grain, while animal protein and small-molecular peptides are increased for distillers ' grains fermentation high protein feed obtained,
Make it closer to complete feed, increases nutritive value.
Further, the quick-acting nitrogen sources further include the silkworm chrysalis peptide or yeast extract with protease hydrolyzed.
It, will be white for the degradation rate for improving cellulose and hemicellulose in coarse-fibred degradation rate, especially vinasse in vinasse
The mixture of vinasse and brewex's grains is heated in 150~180 DEG C.On the one hand, cellulose is unstable at 120 DEG C or so,
150~180 DEG C can degrade, degree of polymerization decline, and chemical composition also changes, and carbonyl increases, on the other hand, in high temperature
In heating process, the moisture content of vinasse is reduced, and can form solid culture medium for subsequent microbial fermentation.
White-rot fungi is a kind of fungi that can secrete extracellular oxidizing ferment lignin degrading, and the ability of lignin degrading is excellent
In the ability of degraded cellulose.The extracellular oxidizing ferment of these energy lignin degradings is respectively lignin peroxidase（LiP）, manganese
Peroxidase (MnP) and laccase (Lac), lignin peroxidase are a series of containing Fe3+, porphyrin ring and prosthetic heme group
Isodynamic enzyme, catalytic action have non-specificity, and substrate specificity is extensive；Manganese peroxidase is a kind of dependence H2O2Ferrous blood
Red pigment glucoproteinase；Laccase is a kind of polyphenol oxidase of cupric, these three enzymes are using a metal ion species as prothetic group
Metalloenzyme mutually cooperates in this function of lignin degrading.The prior art usually selects Phanerochaete chrysosporium for degrading
Lignin in distillers ' grains, it is adaptable, it generates enzyme system and stablizes, the ability with depth degradation lignin, but due to fermentation temperature
Degree, PH, lignin and hemicellulose level, fibre crystallinity, mesostate, zymoexciter and inhibit inhibitor in substrate
The influence of equal many factors, degradation efficiency are low.
The degradation enzyme system of Phanerochaete chrysosporium lacks laccase, and lignin peroxidase, manganese peroxidase need
H2O2Under conditions of competence exertion act on, and laccase does not have limitation with these conditions, the produced laccase activity height of Pleurotus eryngii, and laccase exists
Vigor highest when 30 DEG C, PH 7, stability is more preferable, using Pleurotus eryngii bacterium and both white-rot fungis of Phanerochaete chrysosporium as
One strain, with 2：1~3：1 proportion while the level-one degradation for being applied to lignin, can act synergistically, to improve the drop of lignin
Solution rate.28~32 DEG C of temperature, PH 6.0~7.0, humidity 82~88% fermentation condition under ferment 4~6d.
Further, the oxygen-supply quantity of the first strain fermentation early stage is controlled 5：2~7：1, ferment middle control exists
1：1~3：1, fermentation advanced stage control is 4：1~4：3.
Further, Cu is added in the fermentation medium of level-one degradation2+ , reduced glutathione, both for
Zymoexciter serves as a connection when enzyme-to-substrate combines, promotes the degradation of lignin.
The degradation of cellulose and hemicellulose relies primarily on the cellulase of the microorganism secretions such as bacterium and fungi, cellulose
Enzyme can be divided into 3 major class according to its catalysis：Circumscribed-beta-1,4-glucan enzyme（exo-β-1,4-glucanases）, inscribe-β-
1,4- dextranase（endo-β-1,4-glucanases）And beta-glucosidase（β-1,4-glucosidases）.Study table
Bright, the thorough degradation of avicel cellulose at least needs the synergistic effect of this 3 groups of cellulases：The cellulolytic crystallization of excision enzyme
Area, from the reducing end of cellulose chain（reducing end）Or non-reducing end（non-reducing end）Start continued hydrolysis,
Cellobiose is discharged, is the most important condition for hydrolyzing native cellulose substrate；Restriction endonuclease mainly acts on the noncrystalline domain of cellulose,
Glycosidic bond in random hydrolysis cellulose chain cuts off the long-chain of cellulose, becomes the short chain of cellulose of a large amount of different polymerization degrees,
So that the degree of polymerization of cellulosic molecule reduces, increase for the cellulose chain end number of circumscribed enzyme effect；Beta-glucosidase is then
Main hydrolysis fiber disaccharides and soluble cellooligsaccharides, finally convert cellulose into available glucose.
The producing strains of cellulase mainly have trichoderma, aspergillus and a mould, and second level degradation of the invention is with trichoderma reesei and black
Aspergillus degraded cellulose and hemicellulose, with 2：1~4：1 proportion is fermented, 26~30 DEG C of temperature, PH 5.0~6.5,
Ferment 3~5d under the fermentation condition of humidity 85~90%, and the oxygen-supply quantity of the second level degradable fermented early stage is controlled 4：1~6：
1, ferment middle is controlled 1：1~2：1, fermentation advanced stage control is 4：3~3：1.
Trichoderma reesei and aspergillus niger do not generate toxin and antibiotic under condition of enzyme production, and under the fermentation condition, inner
The produced cellobiase of family name's trichoderma and the produced exoglucanase amount of aspergillus niger are big, vigor is high, enzyme stability is good.Avicel cellulose
Degradation be main rate-limiting step during entire cellulose degradation, the increase of exoglucanase can make avicel cellulose fast
Quick-release puts cellobiose, quickly provides substrate for successive depths degradation, improves degradation speed and palliating degradation degree；Cellobiase
Increase cellulose intermediate metabolites cellobiose can be made further to degrade, avoid cellobiose gather and to enzymatic reaction generate
Inhibiting effect, to improve degradation speed and degradation rate.
Further, it is stirred in the ferment middle of level-one degradation, second level degradation with the speed of 5-8r/min, makes thallus
And its cellulase of secretion comes into full contact with culture medium, while dispersing the mesostate of thallus generation, makes in local
Between metabolite content reduce, mitigate mesostate to the inhibiting effect of enzymatic reaction, promote fiber depth degradation, thus
Improve degradation rate.
Brewex's grains are the by-products using barley as raw material, through Mashing process, after direct squeeze and filter.Crude protein in brewex's grains
Matter content accounts for the 22%~27% of dry weight, and crude fat accounts for 6%~8%, and linoleic acid accounts for 3.23%, full of nutrition, and crude fiber content is low,
It can be used as most of feed for storing fowl；Brewex's grains do not pass through brewing fermentation process, only saccharification grain, and the inside has also remained largely
Maltose and glucose, nitrogen-free extracts accounts for 39%~48%, can be used as the carbon source of microbial fermentation, and enriching iron is microorganism
A great number of elements needed for growth, breeding, manganese, copper are microelements needed for microorganism growth, breeding, can be used as the activation of enzyme
Agent, while being also the component of part enzyme.But brewex's grains easily moldy metamorphism can mix fresh beer vinasse with wheat bran, by 5：1
~8：1 proportion, which is mixed into distillers ' grains, ferments, and both provides carbon source for distillers ' grains fermentation, compensates for nitrogen-free in distillers ' grains and soaks
The low deficiency of apparent extract, and solve the problems, such as that fresh beer vinasse is not easy to store.
Further, it can will be added in fresh beer vinasse containing the sugar-cane juice of a large amount of sucrose, glucose and fructose, be conducive to micro- life
Object early period increases rapidly.
Further, vinasse are after trichoderma reesei and fermentation of Aspergillus niger, in the beer being wherein added after impurity elimination, de- hardship
Waste yeast.Beer waste yeast main component is active S.cerevisiae, and there are also a small amount of malt, rice disintegrating slag, includes 48%~55%
Protein, 23%~28% carbohydrate, 6%~8% ribonucleic acid, 2% B family vitamin, 1% glutathione, and
Amino acid abundant and several mineral materials.Through impurity elimination, de- bitter, after inactivation beer waste yeast, nutritional additive addition can be used as
Into lees feeds of the invention, it had not only been effectively utilized the by-product of brewing, but also has increased the egg of lees feeds of the present invention
Bai Hanliang and comprehensive nutrient value.
In high protein lees feeds preparation method of the invention, using brewex's grains as the carbon source of microbial fermentation, both more
The deficiency of glucide in distillers ' grains has been mended, and has solved the problems, such as that fresh beer vinasse is not easy to store, abundant iron is that microorganism is raw
A great number of elements needed for long, breeding, manganese, copper are the microelements that Institute of Micro-biology needs；Using ammonium salt, dried silkworm chrysalis meal as microorganism
Growth, breeding quick-acting nitrogen sources and imitate nitrogen source late, promote distillers ' grains in coarse-fibred degradation, while for lees feeds increase it is dynamic
Physical property albumen makes its nutritional ingredient more tend to complete feed；During the fermentation, first with the extracellular of Phanerochaete chrysosporium secretion
Peroxidase lignin degrading eliminates the covalent bond between lignin and hemicellulose, releases cellulose and hemicellulose,
And with cellulose degraded partial lignin and hemicellulose, then with the stronger trichoderma reesei of cellulose degradation ability and aspergillus niger
Joint Phanerochaete chrysosporium degraded cellulose and hemicellulose, in a manner of step fermentation successively in vinasse lignin,
Cellulose and hemicellulose carry out depth degradation.
Compared with prior art, high protein lees feeds preparation method of the invention fully utilizes distillers ' grains, brewex's grains
With these three brewing industry by-products of beer waste yeast, step fermentation is carried out to vinasse using multi-cultur es, is improved thick in vinasse
The degradation rate and protein content of fiber, to increase the Feeding Value of lees feeds；It is made by fermentation process of the invention
High protein lees feeds crude fiber content reduce 12-15%, crude protein content increase 25-28%, nutrition is more comprehensively.
Specific embodiment
Technical solution of the present invention is described in detail below in conjunction with embodiment.Following embodiment is only used for more
Technical solution of the present invention is clearly demonstrated, therefore is only used as example, and not intended to limit the protection scope of the present invention.
Embodiment 1
A kind of preparation method of high protein lees feeds, includes the following steps：
（1）Pretreatment, by fresh distillers ' grains 150 DEG C at a temperature of be heated to moisture content be 23%, to the hair after drying
Ferment raw material adds wheat bran and is used as carbon source, and the weight proportion of fermentation raw material and wheat bran is 5：1, addition ammonium salt as quick-acting nitrogen sources, with
And weight is equivalent to the dried silkworm chrysalis meal of fresh distillers ' grains weight 12% as effect nitrogen source late, adjustment pH is 6.0, and temperature is 30 DEG C, humidity
It is 82%, obtains vinasse fermentation culture medium, the fermentation medium is subjected to sterilization treatment；
（2）First strain is inoculated into the fermentation medium after sterilizing by level-one degradation, and first strain is that yellow archespore hair is flat
Lead fungi, inoculum concentration 7%, fermentation early stage oxygen-supply quantity control is 7：2, ferment middle is controlled 2：1, fermentation advanced stage control is 4：1,
Level-one degradation feed is obtained after fermentation 6d；
（3）Second level degradation, reconcile level-one degradation feed temperature be 26 DEG C, PH 6.0, humidity 87%, access the second strain,
I.e. proportion is 4：1 trichoderma reesei and aspergillus niger, fermentation early stage oxygen-supply quantity control is 6：1, ferment middle is controlled 2：1, fermentation
Advanced stage controls 4：Second level degradation feed is obtained after 3, the 4d that ferments, and after second level degradation feed inactivation, is neutralized with limewash,
Up to high protein lees feeds after drying.
Embodiment 2
A kind of preparation method of high protein lees feeds, includes the following steps：
（1）Pretreatment, by fresh distillers ' grains and fresh beer vinasse with 2：1 mixing after, 180 DEG C at a temperature of heated, obtain water
The fermentation raw material that point content is 20%, adds wheat bran thereto, and the weight proportion of fermentation raw material and wheat bran is 6：1, add ammonium salt
The dried silkworm chrysalis meal for being equivalent to fresh distillers ' grains weight 10% as quick-acting nitrogen sources and weight imitates nitrogen source as slow, and adjustment pH is 6.5, temperature
Degree is 28 DEG C, humidity 84%, obtains vinasse fermentation culture medium, and the fermentation medium is carried out sterilization treatment；
（2）First strain is inoculated into the fermentation medium after sterilizing by level-one degradation, and first strain is that yellow archespore hair is flat
Lead fungi, inoculum concentration 5%, fermentation early stage oxygen-supply quantity control is 4：1, ferment middle is controlled 1：1, fermentation advanced stage control is 2：1；
Level-one degradation feed is obtained after fermentation 5d；
（3）Second level degradation, reconcile level-one degradation feed temperature be 29 DEG C, PH 6.5, humidity 85%, access the second strain,
I.e. proportion is 3：1 trichoderma reesei and aspergillus niger, fermentation early stage oxygen-supply quantity control is 4：1, ferment middle is controlled 1：1, fermentation
Advanced stage controls 3：Second level degradation feed is obtained after 1, the 5d that ferments, and after second level degradation feed inactivation, is neutralized with limewash,
Up to high protein lees feeds after drying.
Embodiment 3
A kind of preparation method of high protein lees feeds, it is characterised in that：Include the following steps：
（1）Pretreatment, by fresh distillers ' grains, fresh beer vinasse and sugar-cane juice with 12：4：1 mixing after, 160 DEG C at a temperature of added
Heat obtains the fermentation raw material that moisture content is 24%, adds wheat bran thereto, and the weight proportion of fermentation raw material and wheat bran is 6：1,
Addition ammonium salt is equivalent to the dried silkworm chrysalis meal of fresh distillers ' grains weight 14% as effect nitrogen source late, adjustment as quick-acting nitrogen sources and weight
PH is 7.0, and temperature is 32 DEG C, humidity 88%, obtains vinasse fermentation culture medium, and the fermentation medium is carried out sterilization treatment；
（2）Level-one degradation, by the first strain be inoculated into sterilizing after fermentation medium in, first strain be Pleurotus eryngii bacterium and
Phanerochaete chrysosporium, inoculum concentration are respectively 2% and 8%, and fermentation early stage oxygen-supply quantity control is 5：1, ferment middle is controlled 2：
1, fermentation advanced stage control is 3：Level-one degradation feed is obtained after 1, the 4d that ferments；
（3）Second level degradation, reconcile level-one degradation feed temperature be 28 DEG C, PH 5.5, humidity 90%, access the second strain,
I.e. proportion is 2：1 trichoderma reesei and aspergillus niger, fermentation early stage oxygen-supply quantity control is 5：1, ferment middle is controlled 3：2, fermentation
Advanced stage controls 2：Second level degradation feed is obtained after 1, the 3d that ferments, and after second level degradation feed inactivation, is neutralized with limewash,
Up to high protein lees feeds after drying.
Embodiment 4
A kind of preparation method of high protein lees feeds, includes the following steps：
（1）Pretreatment, by fresh distillers ' grains and fresh beer vinasse with 7：2 mixing after, 170 DEG C at a temperature of heated, obtain water
The vinasse mixture that point content is 23%, adds wheat bran thereto, and the weight proportion of fermentation raw material and wheat bran is 7：1, add ammonium
The dried silkworm chrysalis meal that salt and yeast extract are equivalent to fresh distillers ' grains weight 13% as quick-acting nitrogen sources and weight imitates nitrogen source as slow,
Adjusting pH is 7.0, and temperature is 31 DEG C, humidity 86%, obtains vinasse fermentation culture medium, the fermentation medium is sterilized
Processing；
（2）Level-one degradation, by the first strain be inoculated into sterilizing after fermentation medium in, first strain be Pleurotus eryngii bacterium and
Phanerochaete chrysosporium, inoculum concentration are respectively 1% and 5%, and fermentation early stage oxygen-supply quantity control is 5：1, ferment middle is controlled 1：
1, fermentation advanced stage control is 2：1, the fermentation medium is stirred with the speed of 5r/min in ferment middle, after the 6d that ferments
Obtain level-one degradation feed；
（3）Second level degradation, reconcile level-one degradation feed temperature be 30 DEG C, PH 6.5, humidity 86%, access the second strain,
I.e. proportion is 2：1 trichoderma reesei and aspergillus niger, fermentation early stage oxygen-supply quantity control is 4：1, ferment middle is controlled 1：1, fermentation
Advanced stage controls 3：1, level-one degradation feed is stirred with the speed of 8r/min in ferment middle, is obtained after the 5d that ferments
Second level degradation feed is neutralized, up to high protein lees feeds after drying after second level degradation feed inactivation with limewash.
Embodiment 5
The present embodiment and the difference of embodiment 4 be, the quick-acting nitrogen sources are ammonium salt and silkworm chrysalis peptide, the Pleurotus eryngii bacterium and yellow spore
The mass ratio of the flat lead fungi of raw wool is 1：3.
Embodiment 6
The present embodiment and the difference of embodiment 4 are, in the step（3）Second level degradation feed inactivation before, will be through impurity elimination, de-
Beer waste yeast after hardship is added in second level degradation feed, and the additional amount of the beer waste yeast is the 5% of fresh distillers ' grains weight.
Embodiment 7
The present embodiment and the difference of embodiment 6 are, in the step（2）Fermentation medium in Cu is added2+, the beer
The additional amount of waste yeast is the 8% of fresh distillers ' grains weight.
Embodiment 8
The present embodiment and the difference of embodiment 6 are, in the step（2）Fermentation medium in be added reduced form gluathione
Peptide, the additional amount of the beer waste yeast are the 7% of fresh distillers ' grains weight.
Claims (10)
1. a kind of preparation method of high protein lees feeds, it is characterised in that：Include the following steps：
（1）Pretreatment, by fresh distillers ' grains 150~180 DEG C at a temperature of heat, obtain moisture content be 20%~24%
Fermentation raw material, to the fermentation raw material addition wheat bran after the drying as carbon source, the weight of the fermentation raw material and wheat bran is matched
Than being 5：1~8：1, ammonium salt, which is added, as quick-acting nitrogen sources and weight into the fermentation raw material is equivalent to fresh distillers ' grains weight
10~14% dried silkworm chrysalis meal is 6.0~7.0 as effect nitrogen source late, adjustment pH, and temperature is 28~32 DEG C, humidity is 82~88%, is obtained
To vinasse fermentation culture medium, the fermentation medium is subjected to sterilization treatment；
（2）Level-one degradation, the first strain is inoculated into the fermentation medium after the sterilizing, and first strain is yellow archespore
The flat lead fungi of hair, inoculum concentration are 5~8%, and level-one degradation feed is obtained after the 4~6d that ferments；
（3）Second level degradation, the temperature for adjusting level-one degradation feed is 26~30 DEG C, PH is 5.0~6.5, humidity be 85~
90%, the second strain is accessed, i.e., proportion is 2：1~4：1 trichoderma reesei and aspergillus niger, ferment 3~5d after obtain second level degradation raise
Material is neutralized, up to high protein lees feeds after drying after second level degradation feed inactivation with limewash.
2. a kind of preparation method of high protein lees feeds according to claim 1, it is characterised in that：In the step
（1）In, fermentation raw material further includes fresh beer vinasse, and the weight proportion of fresh distillers ' grains and fresh beer vinasse is 2：1~4：1.
3. a kind of preparation method of high protein lees feeds according to claim 2, it is characterised in that：The step（1）
In, before the fermentation raw material is heated, it is added sugar-cane juice into the fermentation raw material, the sugar-cane juice and fresh beer vinasse
Proportion is 3：1~4：1.
4. a kind of preparation method of high protein lees feeds according to claim 1, it is characterised in that：The quick-acting nitrogen sources
It further include silkworm chrysalis peptide or yeast extract.
5. a kind of preparation method of high protein lees feeds according to claim 3, it is characterised in that：The step（2）
In, the oxygen-supply quantity for early stage of fermenting is controlled 5：2~7：1, ferment middle is controlled 1：1~3：1, fermentation advanced stage control is 4：1~
4：3；
The step（3）In, the oxygen-supply quantity for early stage of fermenting is controlled 4：1~6：1, ferment middle is controlled 1：1~2：1, fermentation
Advanced stage controls 4：3~3：1.
6. a kind of preparation method of high protein lees feeds according to claim 1, it is characterised in that：First strain
It further include Pleurotus eryngii bacterium, the mass ratio of the Pleurotus eryngii bacterium and Phanerochaete chrysosporium is 1：5~1：3.
7. a kind of preparation method of high protein lees feeds according to claim 6, it is characterised in that：In the step
（2）Ferment middle the fermentation medium is stirred with the speed of 5-8r/min, or in the step（3）Fermentation
Mid-term is stirred level-one degradation feed with the speed of 5-8r/min.
8. a kind of preparation method of high protein lees feeds according to claim 1, it is characterised in that：In the step
（2）Fermentation medium in Cu is added2+Or reduced glutathione.
9. a kind of preparation method of high protein lees feeds according to claim 1, it is characterised in that：In the step
（3）Second level degradation feed inactivation before, the beer waste yeast after impurity elimination, de- bitter is added in the second level degradation feed, institute
The additional amount for stating beer waste yeast is the 5~8% of fresh distillers ' grains weight.
10. a kind of high protein lees feeds, it is characterised in that：The high protein lees feeds are by any right of claim 1-9
It is required that the preparation method is made.
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| CN112931687A (en) * | 2021-03-19 | 2021-06-11 | 浙江理工大学 | Method for converting wheat straw into feed cooperatively through Fenton reaction pretreatment and inonotus obliquus solid fermentation |
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| CN114468125A (en) * | 2022-03-24 | 2022-05-13 | 宜宾五粮液股份有限公司 | Preparation method of high-nutrition edible fungus chaff feed |
| CN114468125B (en) * | 2022-03-24 | 2023-09-22 | 宜宾五粮液股份有限公司 | Preparation method of high-nutrition edible fungus chaff feed |
| WO2024003323A1 (en) * | 2022-06-29 | 2024-01-04 | Mushlabs Gmbh | Production of a fungal fermentation medium from brewer's spent grain |
| CN115624091A (en) * | 2022-10-26 | 2023-01-20 | 宜宾市南溪区国科中农生物科技有限公司 | White spirit vinasse feed and preparation method thereof |
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