CN108770515A - A kind of method and its application of collecting seedling of strawberry cuttage and seedling culture - Google Patents

A kind of method and its application of collecting seedling of strawberry cuttage and seedling culture Download PDF

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Publication number
CN108770515A
CN108770515A CN201810474655.0A CN201810474655A CN108770515A CN 108770515 A CN108770515 A CN 108770515A CN 201810474655 A CN201810474655 A CN 201810474655A CN 108770515 A CN108770515 A CN 108770515A
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seedling
sub
stolon
collecting
stem
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CN108770515B (en
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贺冬仙
郑建锋
杜维芬
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China Agricultural University
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China Agricultural University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/10Vegetative propagation by means of cuttings

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  • Life Sciences & Earth Sciences (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention belongs to breeding plant technical fields, specifically disclose a kind of method and its application of collecting seedling of strawberry cuttage and seedling culture.The method of collecting seedling is, is 2~3 leaf, 1 heart in sub- seedling, and when white adventitious root of the new basal part of stem with 0.5~1cm, collect seedling;When collecting seedling, it is close to the sub- new basal part of stem of seedling cripetura to cut sub- seedling from stolon, without the stolon before and after the sub- seedling of cutting, the stolon after collecting seedling before and after sub- seedling is set to be connected by the remaining sub- new basal part of stem of seedling cripetura, so, stolon can continue normal growth, and can generate new sub- seedling so that sub- seedling can be constantly harvested from single stolon.The present invention solves the problems, such as that strawberry is collected seedling in the prior art, and procedure is cumbersome, sub- seedling is of low quality and breeding efficiency is relatively low, the purpose for realizing and persistently harvesting the sub- seedling of high-quality from strawberry stolon, and reduce labor intensity, improve cuttage survival rate and breeding efficiency.

Description

A kind of method and its application of collecting seedling of strawberry cuttage and seedling culture
Technical field
The invention belongs to breeding plant technical fields, specifically, be related to a kind of strawberry cuttage and seedling culture collect seedling method and It is applied.
Background technology
Strawberry is rose family Fragaria herbaceos perennial, and the strawberry cultivated extensively in production belongs to octoploid pineapple grass The certain kind of berries (Fragaria × ananassa Duch.), stolon is thin, internode length, upward at the second section position after stolon is sprouted Shortening stem and leaf are grown, grows adventitious root downwards, the sub- seedling of stolon is formed, continues again at the axil of the sub- seedling of stolon newly formed It sprouts stolon and forms the sub- seedling of next stage stolon, the sub- seedling of stolon can inherit the varietal character of female seedling completely, this is profit The basic principle of strawberry seedling breeding is carried out with stolon.
Currently, the seedling in strawberry stolon source is mainly obtained in production by the method for keeping down the vines of a creeping plant and cuttage two ways.Pressure Climing method:It with the elongation of stolon, keeps down the vines of a creeping plant at stolon even number section position and promotes sub- seedling rooting, do not cut between sub- seedlings at different levels Stolon, sub- seedling can obtain nutrition by stolon from female seedling;But stolon is mutually wound, and operation labor intensity of keeping down the vines of a creeping plant is big, Big boy's seedling mixes, and is unfavorable for cultivating high-quality strong sprout.Cuttage:Stolon growth period waits for every without operation of keeping down the vines of a creeping plant When growing 3~4 sub- seedlings for having a small amount of adventitious root on stolon, sub- seedling both ends stolon is cut, by the sub- seedling of acquisition by big Cuttage root-taking is concentrated after small classification, advantageously reduces labor intensity, improves seedling regularity;But since the seedling age of sub- seedling is different, The blade of larger sub- seedling is excessive, the exposed overlong time of adventitious root and it is downright bad, and the blade and adventitious root compared with boy's seedling are very few, It is unfavorable for surviving.
The Chinese patent application of Publication No. CN103975730A discloses a kind of cuttage and seedling culture method of strawberry, but it is only Choosing has the sub- seedlings of stolon of 2~3 expansion leaves, its lower end is cut into cuttage after wedge shape.Publication No. CN104509363A Chinese patent application disclose a kind of strawberry two-section growing seedlings method, cut the stolon with 3~4 plants of sub- seedlings, it is more to remove sub- seedling Remaining blade, the sub- seedling for cuttage only retain 2 leaf, 1 heart.The Chinese patent application of Publication No. CN106258368A discloses A kind of new method of the efficient sprouting and rooting of strawberry stolon, according to the freshness of sub- seedling adventitious root be classified as early stage and recently The sub- seedling formed, the sub- seedling formed recently is used directly for cuttage, and the sub- seedling of early formation need to remove downright bad adventitious root extremely It is just used for cuttage after capable of seeing health tissues.
However, the seedling age of the sub- seedling of strawberry stolons at different levels differs greatly, existing method needs of collecting seedling divide sub- seedling Grade, the sub- seedling big to seedling age carry out the processing such as defoliation, root relief, and the sub- seedling small to seedling age carries out precision management, and recovery process is cumbersome, Sub- seedling is of low quality, and management difficulty is big after cuttage, and 3~4 sub- seedlings are only harvested on every stolon, and cuttage and seedling culture is inefficient.
Invention content
In order to solve strawberry existing in the prior art collect seedling that procedure is cumbersome, sub- seedling is of low quality and breeding efficiency compared with Low problem, the object of the present invention is to provide a kind of method and its application of collecting seedling of strawberry cuttage and seedling culture, from strawberry stolon The sub- seedling of high-quality is persistently harvested, to achieve the purpose that reduce labor intensity, improve cuttage survival rate and breeding efficiency.
In order to realize the object of the invention, technical scheme is as follows:
In a first aspect, the present invention provides a kind of methods of collecting seedling of strawberry cuttage and seedling culture, when collecting seedling, it is new to be close to sub- seedling cripetura Basal part of stem cuts sub- seedling from stolon, without the stolon before and after the sub- seedling of cutting, makes the stolon before and after sub- seedling after collecting seedling Pass through the remaining sub- new basal part of stem connection of seedling cripetura.
Preferably, being 2~3 leaf, 1 heart in sub- seedling, and when white adventitious root of the new basal part of stem with 0.5~1cm, carry out It collects seedling.
Method of the present invention of collecting seedling does not destroy stolon, and stolon with female Miao Xianglian and continued growth, and can still generate New sub- seedling so that can constantly harvest sub- seedling from single stolon, only there are one giving birth to always on single stolon Long sub- seedling.
Preferably, after female seedling stolon is sprouted, making to crawl by manual intervention, stem apex is horizontal, grows downward or upward.
The method of collecting seedling of the present invention is specially:
(1) stolon management:It is horizontal, downward or upward by the manual intervention stem apex that makes to crawl after female seedling stolon is sprouted Growth.
(2) sub- seedling is harvested:When the sub- seedling of stolon is grown to 2~3 leaf, 1 heart, there are 0.5~1cm white adventitious roots, it is short to be close to its The new basal part of stem that contracts cuts it from stolon, and new basal part of stem carries the white adventitious root of 0.5~1cm, front and back stolon It is normally connected yet by the remaining sub- new basal part of stem of seedling cripetura.Shearing wound heals completely after one day, waits for next stage stolon Sub- seedling is grown to 2~3 leaf, 1 heart, when having 0.5~1cm white adventitious roots, is harvested in the same way.Sub- seedling after harvesting is through skewer Become Original Strawberry seedling or production seedling after inserting culture.
Second aspect, the present invention provides the application of the method in strawberry cuttage and seedling culture of collecting seedling.
Sub- seedling under being adopted using the method for collecting seedling of the present invention is 2~3 leaf, 1 heart, and new basal part of stem carries 0.5~1cm White adventitious root, it is uniform in size consistent, be easy to survive after cuttage, Original Strawberry seedling or production can be become after seedling is cultivated With seedling, cuttage and seedling culture labor intensity can be effectively reduced.
Therefore, the present invention also provides the application of the method in preparing Original Strawberry seedling or production seedling of collecting seedling.
On the basis of common knowledge of the art, above-mentioned each optimum condition, can be combined with each other, obtain specific embodiment party Formula.
The beneficial effects of the present invention are:
Using method strawberrying seedling of the present invention of collecting seedling, can make always to only have on the stolon of mother Miao Xianglian One sub- seedling being growing, is conducive to the management of the fast-growth and stolon of sub- seedling, and the sub- seedling under adopting is 2~3 leaves 1 The heart, and 0.5~1cm adventitious roots are carried, it is uniform in size consistent, it is easy to survive after cuttage, strawberry can be become after seedling is cultivated Original seed seedling or production seedling can effectively reduce cuttage and seedling culture labor intensity.
Moreover, method of the present invention of collecting seedling does not cut the stolon before and after sub- seedling when collecting seedling, and stolon is still With female Miao Xianglian and continued growth, and new sub- seedling can be generated so that sub- seedling can be constantly harvested from single stolon.? The 4-9 months during nursery, 9 or more sub- seedlings can be harvested from highest on single stolon, it can be with compared with tradition collects seedling mode Cuttage and seedling culture efficiency is improved into 2 times or more.
Description of the drawings
Fig. 1 be female seedling crawl the management of stem apex horizontal growth when method schematic diagram of collecting seedling;
Fig. 2 be female seedling crawl stem apex downward growth management when method schematic diagram of collecting seedling;
Fig. 3 is that female seedling is crawled collect seedling method schematic diagram of stem apex when growing up management;
In figure, 1:Female seedling, 2:Stolon, 3:It crawls stem apex, 4:The sub- seedling of stolon, 5:The new stem foot of cripetura of the sub- seedling of stolon Portion, 6:Remain in the new basal part of stem of cripetura on stolon, 7:Adventitious root, 8:The new stem of cripetura, 9:Stolon geometrical clamp.
Specific implementation mode
With reference to embodiment the present invention will be further explained explanation.It will be appreciated that following embodiment provides Merely to playing the purpose of explanation, it is not used to limit the scope of the present invention.Those skilled in the art is not In the case of spirit of the invention and spirit, the present invention can be carry out various modifications and be replaced.
Experimental method used in following embodiments is conventional method unless otherwise specified.
The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
Embodiment 1
Based on practical experiment in cultivation and combine Fig. 1 to female seedling crawl stem apex horizontal growth management when the process of collecting seedling said It is bright.
(1) stolon management:The field planting of female seedling 1 in the side of high-level cultivating bed, cultivating by the stem apex 3 of crawling generated from female seedling Free growth in the horizontal direction on bed.Guide properly is crawled 3 direction of growth in the horizontal direction of stem apex during collecting seedling, it is ensured that it begins Eventually on cultivation bed.
(2) sub- seedling is harvested:When the sub- length of seedling 4 of stolon is close to it to 2~3 leaf, 1 heart when having 0.5~1cm white adventitious roots 7 The new basal part of stem of cripetura 5 cuts it from stolon 2 for cuttage, and white of the new basal part of stem 5 of cripetura with 0.5~1cm is not Determine root 7, front and back stolon 2 remains in yet by the new 6 normally connection of basal part of stem of the cripetura remained on stolon on stolon The shearing wound of the new basal part of stem of cripetura 6 heal completely after one day.With the increase of stolon quantity and length, adopt down in time 2~3 leaf, 1 heart on stolon 2 and the sub- seedling 4 for having 0.5~1cm whites adventitious root 7.Cuttage is in 32 holes of filling substrate after collecting seedling In hole tray, become high-quality strawberry seedling after culture in 20 days.
During annual 4-9 months nursery, 9 or more sub- seedlings can be harvested from highest on single stolon, be that tradition is collected seedling 3 times or more of mode breeding efficiency only need to carry out guide properly during collecting seedling to stolon, and time saving and energy saving, the sub- seedling of harvesting is short The new stem 8 that contracts is L-shaped, fixes and takes root conducive to cuttage.
Embodiment 2
Based on practical experiment in cultivation and combine Fig. 2 crawl to female seedling stem apex downward growth management when the process of collecting seedling said It is bright.
(1) stolon management:Female seedling 1 is colonized in high-level cultivating slot, and the stem apex 3 of crawling generated from female seedling is in gravity Downward free growth down.To prevent from crawling, stem apex 3 contacts ground, and the stolon 2 that will be close to 1 side of female seedling in due course is wound, Gu It is fixed.
(2) sub- seedling is harvested:When the sub- length of seedling 4 of stolon is close to it to 2~3 leaf, 1 heart when having 0.5~1cm white adventitious roots 7 The new basal part of stem of cripetura 5 cuts it from stolon 2, and the new basal part of stem 5 of cripetura carries the white adventitious root 7 of 0.5~1cm, For front and back stolon 2 yet by the new 6 normal connection of basal part of stem of the cripetura remained on stolon, the cripetura remained on stolon is new The shearing wound of basal part of stem 6 heals completely after one day.With the increase of stolon quantity and length, lower stolon 2 is adopted in time On 2~3 leaf, 1 heart and have the sub- seedling 4 of 0.5~1cm whites adventitious root 7.Cuttage is in 32 hole hole trays of filling substrate after collecting seedling, Become high-quality strawberry seedling after culture in 20 days.
During 4-9 month nursery, 9 or more sub- seedlings are harvested from highest on single stolon, are that the tradition mode of collecting seedling is educated 3 times or more of seedling efficiency.Due to crawling, stem apex 3 is grown straight down under the effect of gravity, and the new stem 8 of cripetura that sub- seedling is formed is in J Type facilitates artificial seeding, alleviates labor intensity of collecting seedling.
Embodiment 3
Based on practical experiment in cultivation and combine Fig. 3 to female seedling crawl stem apex grow up management when the process of collecting seedling said It is bright.
(1) stolon management:The field planting of female seedling 1 in the side of high-level cultivating bed, crawling by the stem apex 3 of crawling generated from female seedling It keeps growing straight up under the fixation of stem geometrical clamp 9.
(2) sub- seedling is harvested:When the sub- length of seedling 4 of stolon is close to it to 2~3 leaf, 1 heart when having 0.5~1cm white adventitious roots 7 The new basal part of stem of cripetura 5 cuts it from stolon 2, and the new basal part of stem 5 of cripetura carries the white adventitious root 7 of 0.5~1cm, For front and back stolon 2 yet by the new 6 normal connection of basal part of stem of the cripetura remained on stolon, the cripetura remained on stolon is new The shearing wound of basal part of stem 6 heals completely after one day.The stem apex 3 that will crawl after collecting seedling is fixed in time on stolon geometrical clamp 9, It keeps it to grow straight up, waits for that its length is cut to 2~3 leaf, 1 heart when having 0.5~1cm white adventitious roots 7 in the same way Under.Cuttage becomes high-quality strawberry seedling in 32 hole hole trays of filling substrate after culture in 20 days after collecting seedling.
During 4-9 month nursery, 9 or more sub- seedlings are harvested from highest on single stolon, are that the tradition method of collecting seedling is educated 3 times or more of seedling efficiency.It is grown straight up under the fixation of stolon geometrical clamp 9 due to crawling stem apex 3, the cripetura that sub- seedling is formed New stem 8 is in I types, is advantageously implemented mechanization and collects seedling, and improves efficiency of collecting seedling.
Although above the present invention is described in detail with a general description of the specific embodiments, On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause This, these modifications or improvements, belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.

Claims (7)

1. a kind of method of collecting seedling of strawberry cuttage and seedling culture, which is characterized in that when collecting seedling, be close to the sub- new basal part of stem of seedling cripetura by sub- seedling It is cut from stolon, without the stolon before and after the sub- seedling of cutting, the stolon after collecting seedling before and after sub- seedling is made to pass through remaining son The new basal part of stem connection of seedling cripetura.
2. method according to claim 1 of collecting seedling, which is characterized in that in sub- seedling be 2~3 leaf, 1 heart, and new basal part of stem carries When the white adventitious root of 0.5~1cm, collect seedling.
3. method according to claim 1 or 2 of collecting seedling, which is characterized in that sub- seedling is persistently harvested from single stolon, it is single Only there are one the sub- seedlings being growing always on stolon.
4. method according to claim 3 of collecting seedling, which is characterized in that make the stem apex of crawling that female seedling generates by labor management Level is grown downward or upward.
Application of the method in strawberry cuttage and seedling culture 5. Claims 1 to 4 any one of them is collected seedling.
6. application according to claim 5, which is characterized in that under the method for collecting seedling described in any one of Claims 1 to 4 is adopted Sub- seedling is 2~3 leaf, 1 heart, and new basal part of stem carries the white adventitious root of 0.5~1cm.
Application of the method in preparing Original Strawberry seedling or production seedling 7. Claims 1 to 4 any one of them is collected seedling, It is characterized in that, the sub- seedling under the method for collecting seedling described in any one of Claims 1 to 4 is adopted is used as original seed seedling or production after cuttage culture It is used with seedling.
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Cited By (3)

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Publication number Priority date Publication date Assignee Title
CN109964755A (en) * 2019-05-15 2019-07-05 十堰市经济作物研究所 A method of improving Original Strawberry kind shoot survival percent and quality
CN112930890A (en) * 2021-02-03 2021-06-11 农业农村部规划设计研究院 Strawberry stolon traction and cutting composite seedling culture method
CN113099976A (en) * 2021-04-12 2021-07-13 云南省农业科学院园艺作物研究所 Method for efficiently breeding seedlings of open-field strawberry stolons

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109964755A (en) * 2019-05-15 2019-07-05 十堰市经济作物研究所 A method of improving Original Strawberry kind shoot survival percent and quality
CN109964755B (en) * 2019-05-15 2021-03-23 十堰市经济作物研究所 Method for improving survival rate and quality of strawberry stock seedlings
CN112930890A (en) * 2021-02-03 2021-06-11 农业农村部规划设计研究院 Strawberry stolon traction and cutting composite seedling culture method
CN113099976A (en) * 2021-04-12 2021-07-13 云南省农业科学院园艺作物研究所 Method for efficiently breeding seedlings of open-field strawberry stolons
CN113099976B (en) * 2021-04-12 2023-12-05 云南省农业科学院园艺作物研究所 Method for efficiently breeding seedlings by creeping stems of open field strawberries

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