CN107793263B - Application of efficient phosphate solubilizing bacteria - Google Patents
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Abstract
The invention discloses an application of a high-efficiency phosphate solubilizing bacterium (with the preservation number of CGMCC NO.12767), which is mainly applied to preparing a high-efficiency phosphate fertilizer and a microbial compound seed coating agent. The invention has the advantages that: (1) the prepared high-efficiency phosphate fertilizer can obviously improve soil after being applied, improve the content of available phosphorus in the soil, which can be absorbed and utilized by crops, and further improve the dry weight of the crops by more than 8 percent; (2) the prepared microbial compound seed coating agent can improve the capability of crops for absorbing available phosphorus after being coated on the surface of seeds, and further improves the dry weight of the crops by more than 7%.
Description
Technical Field
The invention relates to application of a high-efficiency phosphate solubilizing bacterium, belonging to the technical field of biology.
Background
Phosphorus is one of the major elements necessary for plant growth and development. Most of phosphorus is fixed on soil aggregates, so that the phosphorus in the soil which can be directly absorbed and utilized by plants is very rare.
Although China is one of the countries with the largest fertilizer consumption all over the world, the phosphorus element which can be utilized by plants in soil is still deficient. Research has shown that soluble phosphorus element content in many soils is 1ppm or less, which greatly restricts plant growth and development. Excessive use of phosphate fertilizer causes various environmental pollution problems such as water eutrophication and the like.
Soil microorganisms are the power for soil nutrient conversion and circulation, and the utilization rate of the soil stationary phosphorus is improved by utilizing the microorganisms, so that the method is an effective method. The phosphate-solubilizing microorganism can secrete low molecular weight organic acid, such as oxalic acid, citric acid, etc., H+Can dissolve mineral phosphorus element in soil and release phosphorus element fixed on soil aggregate, thereby improving the content of soluble phosphorus in soil.
At present, a plurality of fungus strains such as penicillium, aspergillus and the like have been reported to have the effect of dissolving sessile phosphorus in soil. By inoculating microorganisms capable of efficiently dissolving phosphorus elements into soil, the content of effective phosphorus in the soil can be increased, so that the dry weight of plants such as wheat, soybean and the like is increased. Phosphorus solubilizing microorganisms have been studied for application to soil (near the roots of plants) and the co-inoculation of leguminous plant seeds with a phosphorus solubilizing fungus along with rhizobia has also been studied to improve plant phosphorus uptake.
However, microbial strains with high activity for degrading phosphorus, which can improve plant growth conditions and increase the available phosphorus level in soil systems, are still scarce and in wide demand.
Disclosure of Invention
The invention relates to a penicillium strain (preservation number CGMCC NO.12767) which is obtained by separating and purifying corn soil and has the capability of efficiently degrading phosphate.
The invention adopts the following technical scheme:
application of penicillium strain (preservation number CGMCC NO.12767) in preparing high-efficiency phosphate fertilizer
The method for preparing the high-efficiency phosphate fertilizer comprises the following steps:
step 1: fermenting by using efficient phosphate solubilizing bacteria with the preservation number of CGMCC NO.12767 to obtain fermentation liquor;
step 2: preparing half-cooked grains from wheat, corn grit and/or rice;
step 3: inoculating 10% of the fermentation liquid into sterilized half-cooked grains, culturing at 25 deg.C for 5 days, turning over grains after 5 days, and standing for 10 days for 15 days;
step 4: culturing for 15 days, drying, pulverizing, and sieving to obtain fungal spore powder;
step 5: mixing the fungal spore powder with grass peat, light calcium carbonate and a phosphorus source which are sterilized at high temperature, wherein the weight ratio of the grass peat to the light calcium carbonate to the phosphorus source is 1000:4000:10:100, stirring evenly and crushing to obtain the high-efficiency phosphate fertilizer.
The method described above, characterized in that in Step1, the fermentation process is:
(1) inoculating the penicillium strain with the preservation number of CGMCC NO.12767 to the slant of an MEA culture medium, and culturing at 28 ℃ for 7 days to obtain slant seeds;
(2) inoculating the slant seeds into a liquid culture medium, wherein the liquid culture medium comprises the following components: 30g of cane sugar, 30g of soybean meal and FeSO4·7H200.005g+MnSO40.008g+H3BO30.002g+ZnSO40.005g+CuSO40.004g+Na2MoO40.004g+CoCl20.002g and 0.04g inositol, adding distilled water to 1L, culturing at 29 deg.C with initial pH of 5-6 to obtain liquid seed;
(3) inoculating liquid seeds into a fermentation tank, wherein the fermentation medium comprises the following components: 30g of cane sugar, 30g of soybean meal and FeSO4·7H200.005g+MnSO40.008g+H3BO30.002g+ZnSO40.005g+CuSO40.004g+Na2MoO40.004g+CoCl20.002g + inositol 0.04g, adding distilled water to 1L, the initial pH value is 5-6, and the culture conditions are as follows: fermenting at 26-32 deg.C and stirring speed of 150-250rpm for 4-6 days to obtain fermentation liquid.
The method described above is characterized in that the process for producing half-cooked cereal grains in Step2 is:
soaking wheat, corn grit and/or rice in warm water at 55 deg.C for a period of time until the water content of the grain is 30%, and aging at 100 deg.C for 10 min to obtain half-cooked grain.
Application of penicillium strain (preservation number CGMCC NO.12767) in preparation of microbial compound seed coating agent
The method for preparing the microbial compound seed coating agent comprises the following steps:
step 1: fermenting by using efficient phosphate solubilizing bacteria with the preservation number of CGMCC NO.12767 to obtain fermentation liquor;
step 2: preparing half-cooked grains from wheat, corn grit and/or rice;
step 3: inoculating 10% of the fermentation liquid into sterilized half-cooked grains, culturing at 25 deg.C for 5 days, turning over grains after 5 days, and standing for 10 days for 15 days;
step 4: culturing for 15 days, drying, pulverizing, and sieving to obtain fungal spore powder;
step 5: adding the film forming agent into warm water of 50 ℃, stirring until the film forming agent is completely dissolved, continuously adding the insecticide, the dispersing agent, the protective agent, the coloring agent and the nutritional agent into the warm water, uniformly mixing, then grinding for 4 hours in a sand mill to prepare a suspending agent, finally adding the fungal spore powder into the suspending agent, and uniformly mixing to obtain the microbial compound seed coating agent.
The method described above, characterized in that in Step1, the fermentation process is:
(1) inoculating the penicillium strain with the preservation number of CGMCC NO.12767 to the slant of an MEA culture medium, and culturing at 28 ℃ for 7 days to obtain slant seeds;
(2) inoculating the slant seeds into liquid culture mediumThe liquid medium consists of: 30g of cane sugar, 30g of soybean meal and FeSO4·7H200.005g+MnSO40.008g+H3BO30.002g+ZnSO40.005g+CuSO40.004g+Na2MoO40.004g+CoCl20.002g and 0.04g inositol, adding distilled water to 1L, culturing at 29 deg.C with initial pH of 5-6 to obtain liquid seed;
(3) inoculating liquid seeds into a fermentation tank, wherein the fermentation medium comprises the following components: 30g of cane sugar, 30g of soybean meal and FeSO4·7H200.005g+MnSO40.008g+H3BO30.002g+ZnSO40.005g+CuSO40.004g+Na2MoO40.004g+CoCl20.002g + inositol 0.04g, adding distilled water to 1L, the initial pH value is 5-6, and the culture conditions are as follows: fermenting at 26-32 deg.C and stirring speed of 150-250rpm for 4-6 days to obtain fermentation liquid.
The method described above is characterized in that the process for producing half-cooked cereal grains in Step2 is:
soaking wheat, corn grit and/or rice in warm water at 55 deg.C for a period of time until the water content of the grain is 30%, and aging at 100 deg.C for 10 min to obtain half-cooked grain.
The method is characterized in that in Step5, the dosage of each raw material for preparing the microbial compound seed coating agent is as follows by mass percent:
the method is characterized in that the pesticide is any one or a mixture of any more of metalaxyl-M, Yidaam, thiram, iprodione, imidacloprid, triticonazole, cypermethrin, tebuconazole, chlordiazepoxide and flutriafol.
The invention has the advantages that:
(1) the prepared high-efficiency phosphate fertilizer can obviously improve soil after being applied, improve the content of available phosphorus in the soil, which can be absorbed and utilized by crops, and further improve the dry weight of the crops by more than 8 percent;
(2) the prepared microbial compound seed coating agent can improve the capability of crops for absorbing available phosphorus after being coated on the surface of seeds, and further improves the dry weight of the crops by more than 7%.
Detailed Description
The present invention will be described in detail with reference to the following embodiments.
Separating and purifying to obtain penicillium strain with high-efficiency phosphate solubilizing capability
We have collected different plant rhizosphere soil widely in the whole country, separated 125 strains of phosphate solubilizing microorganisms, tested the phosphate solubilizing ability, the strain with the number of MN10003 has the strongest phosphate solubilizing ability.
1. Separation and purification process
The separation and purification process of the strain with the number of MN10003 is as follows:
taking the soil of a corn field in Gaomi city of Shandong province as a sample. Weighing 5g of soil sample into a 25ml centrifuge tube, adding 15ml of sterile 0.1% Tween 80 solution, fully shaking and uniformly mixing, and adding 10 g of sample-1、10-2、10-3、10-4、10-5And (5) diluting with a concentration gradient. Aspirate 2ml 10-4The diluted solution was uniformly spread on a 9 cm-diameter plate containing PDA (potato dextrose) medium to which streptomycin and 100ppm chloramphenicol were added to a final concentration of 100 ppm. The plates were incubated at 25 ℃ in the dark for 5 days and checked daily for the appearance of new colonies. After new colonies appear, the colonies are purified, hyphae are picked at the edges of the colonies and are inoculated in a new PDA plate in time to form pure fungus colonies.
2. Test of phosphorus dissolution ability
(1) Efficiency of phosphorus solubilization on different phosphorus source media
By taking Penicillium bilaiae ATCC20851 and Bacillus megaterium ATCC14581 as control strains and taking a strain with the number of MN10003 as a test strain, phosphorus dissolving rings (mm) and colony growth diameters (mm) of the three strains on phosphorus dissolving culture media with different phosphorus sources are compared, and the results are as follows:
the results show that: the strain with the number of MN10003 has the degradation capability on both organic phosphorus sources and inorganic phosphorus sources.
(2) The phosphate dissolving effect of the strain with the number of MN10003 in a liquid culture medium with indissolvable phosphate as a unique phosphorus source
Adding the strain with the number of MN10003 and the Penicillium bilaiae ATCC20851 into a liquid culture medium which takes difficultly soluble phosphate as a unique phosphorus source, culturing at 27 ℃ for 10 days, and taking blank non-inoculated bacteria as a Control (CK).
The formula of the liquid culture medium is as follows: (NH)4)2SO4 0.5g/L，NaCl 0.2g/L，KCl 0.2g/L，MgSO4 7H2O 0.03g/L，MnSO4 0.03g/L，FeSO40.003g/L, 0.5g/L yeast powder, 10.0g/L glucose and 5.0g/L ground phosphate rock (screened by a 100-mesh sieve).
In the liquid culture medium, the phosphate solubilizing rates of different phosphate solubilizing fungi are as follows:
the results show that:
(1) MN10003 and ATCC20851 both have obvious phosphorus dissolving effect and obvious difference between strains;
(2) when the nitrogen source is ammonium nitrogen, MN10003 is cultured for 10 days, the water-soluble phosphorus in 50ml of culture medium is 10.3mg, accounts for 41.2 percent of the total amount of the applied phosphate rock powder and is obviously higher than ATCC 20851;
(3) when the nitrogen source is nitrate nitrogen, MN10003 is cultured for 10 days, and the water-soluble phosphorus in 50ml of culture medium is 13.9mg and accounts for 55.6 percent of the total weight of the ground phosphate rock.
3. Endurance test
Using Penicillium bilaiae ATCC20851 and Bacillus megaterium ATCC14581 as control strains and the strain with the number MN10003 as test strains, we tested the semi-lethal concentrations LC50 of the three strains for different chemicals, with the following results (unit: mg/ml):
the results show that: the strain with the number of MN10003 has stronger tolerance to the existing chemical pesticide.
In conclusion, compared with the reported strains, the strain with the number of MN10003 has stronger capability of dissolving phosphorus elements in soil and stronger tolerance, can be used with various chemical pesticides in a synergistic manner, and has wide application prospect.
4. Effect on corn growth
The bacterial strain with the number of MN10003 is prepared into a microbial inoculum by a conventional method, and after the bacterial strain is cultured for 30 days in different soil environments, the biomass of corn in a pot is tested, and the test result is as follows (unit: g/pot):
in the table, the soil No.1 is taken from farmlands in Yadu areas of Shanxi province, is light soil moisture and has 17.9 mug/g of available phosphorus; no. 2 soil is taken from millstone ridge farmland in Quwa county of Shanxi province, is medium-soil moisture soil and has 10.3 mu g/g of available phosphorus; no. 3 soil is taken from millstone ridge in Quwa county of Shanxi province, and is laid on a wasteland for many years, and available phosphorus is 5.1 mu g/g.
The results show that: the strain with the number of MN10003 has the effect of promoting the growth of the corn.
5. Preservation of
In 2016, 7 months and 12 days, a strain with the number of MN10003 is preserved in the China general microbiological culture collection center, the preservation address is No. 3 of Xilu No.1 of Beijing Korean district, the preservation number is CGMCC NO.12767, and the classification names are as follows: penicillium sp.
Secondly, fermenting by using the penicillium strain
1. Slant seed culture in test tubes
The Penicillium strain (preservation number CGMCC NO.12767) is inoculated on the inclined plane of an MEA culture medium (30 g of malt extract, 3g of soybean peptone and 15g of agar, distilled water is added to prepare 1L of solution, and then the solution is sterilized at 121 ℃ for 15min, and the pH is adjusted to 5.6 +/-0.2). Slant seeds were obtained after 7 days of culture at 28 ℃.
2. Liquid seed culture of bacterial strains
Liquid culture medium: 30g of cane sugar, 30g of soybean meal and FeSO4·7H200.005g+MnSO40.008g+H3BO30.002g+ZnSO40.005g+CuSO40.004g+Na2MoO40.004g+CoCl20.002g of inositol and 0.04g of distilled water are added to prepare 1L, and the initial pH value is 5-6.
Incubate to the appropriate concentration at 29 ℃. The air flux is properly increased in the culture process, which is beneficial to the liquid fermentation of the strain to produce a large amount of spores.
3. Mass culture in strain fermentation tank
Fermentation medium: 30g of cane sugar, 30g of soybean meal and FeSO4·7H200.005g+MnSO40.008g+H3BO30.002g+ZnSO40.005g+CuSO40.004g+Na2MoO40.004g+CoCl20.002g of inositol and 0.04g of distilled water are added to prepare 1L, and the initial pH value is 5-6.
Inoculating liquid seeds into a (20-1000L) fermentation tank according to the proportion of 1-5% (V/V), and culturing under the following conditions: fermenting at 26-32 deg.C and stirring speed of 150-250rpm for 4-6 days to obtain fermentation liquid.
Thirdly, preparing fungal spore powder by using the fermentation liquor
1. Preparation of half-cooked grains
Soaking wheat, corn grit and/or rice in 55 deg.C warm water for a period of time (about 40 minutes) until the water content of the grain is 30%, and aging at 100 deg.C for 10 minutes to obtain half-cooked grain.
The half-cooked grains were sterilized at 121 ℃ under 101kPa for 90 minutes for use.
2. Inoculating and culturing
Inoculating the fermentation liquid into sterilized half-cooked grains at a ratio of 10%, culturing at 25 deg.C for 5 days, turning over grains after 5 days, and standing for 10 days for 15 days.
3. Obtaining fungal spore powder
After culturing for 15 days, the culture is dried, crushed and sieved to obtain fungal spore powder.
The detection shows that the concentration of effective viable bacteria in the spore powder is 4 multiplied by 108Per gram.
Fourthly, preparing high-efficiency phosphate fertilizer by utilizing fungal spore powder
Mixing the fungal spore powder with grass peat, light calcium carbonate and a phosphorus source which are sterilized at high temperature according to the weight ratio of 1000:4000:10:100, uniformly stirring, and crushing to obtain the high-efficiency phosphate fertilizer. Wherein the phosphorus source can be either an organic phosphorus source (e.g., lecithin) or an inorganic phosphorus source (e.g., Ca)8H2PO4)。
Selecting light loam from farmland in Yadu district of Shanxi province, wherein the effective phosphorus content is 17.9 mug/g, applying the high-efficiency phosphate fertilizer for 60 days, testing the dry weight of crops, and further calculating the increase rate of the dry weight of the crops. The calculation results are as follows:
therefore, the high-efficiency phosphate fertilizer can obviously improve the dry weight of crops.
Fifthly, preparing microbial compound seed coating agent by utilizing fungal spore powder
1. Active ingredient
Fungal spore powder and pesticide (one or mixture of more of metalaxyl-M, Yidaam, thiram, iprodione, imidacloprid, triticonazole, cypermethrin, tebuconazole, chlordiazepoxide and flutriafol).
2. Auxiliary materials
Protective agent (glycerin), colorant (sunset yellow), nutritional agent (sodium sulfate), film forming agent (any one or mixture of xanthan gum, sodium carboxymethylcellulose, chitosan and starch), and dispersant (agricultural milk 602 and/or 2-3% NNO).
3. Formula (in percentage by mass)
| Fungal spore powder | 14-26％ |
| Insecticide | 1-5％ |
| Protecting agent | 1-4％ |
| Coloring agent | 1-3％ |
| Nutrient agent | 2-6％ |
| Film forming agent | 20-60％ |
| Dispersing agent | 2-3％ |
| Water (W) | Make up to 100% |
4. Preparation method
Adding the film forming agent into warm water of 50 ℃, stirring until the film forming agent is completely dissolved, continuously adding the insecticide, the dispersing agent, the protective agent, the coloring agent and the nutritional agent into the warm water, uniformly mixing, then grinding for 4 hours in a sand mill to prepare a suspending agent, adding fungal spore powder into the suspending agent, and uniformly mixing to obtain the microbial compound seed coating agent.
5. Effects of use
Group 1: 14% of fungal spore powder, 1% of pesticide, 1% of protective agent, 1% of colorant, 2% of nutritional agent, 20% of film-forming agent, 2% of dispersing agent and 59% of water.
Group 2: 20% of fungal spore powder, 3% of insecticide, 2% of protective agent, 2% of colorant, 4% of nutrient, 30% of film-forming agent, 2% of dispersant and 37% of water.
Group 3: 26% of fungal spore powder, 5% of insecticide, 4% of protective agent, 3% of colorant, 6% of nutrient, 40% of film-forming agent, 3% of dispersant and 13% of water.
After the seed coating agent of the invention is used for 60d, the dry weight of crops is tested, and then the growth rate of the dry weight of the crops is calculated. The calculation results are as follows:
therefore, the seed coating agent can obviously improve the dry weight of crops.
It should be noted that the above-mentioned embodiments do not limit the present invention in any way, and all technical solutions obtained by using equivalent alternatives or equivalent variations fall within the protection scope of the present invention.
Claims (6)
1. The application of the high-efficiency phosphate solubilizing bacteria in preparing the high-efficiency phosphate fertilizer is characterized in that the preservation number of the high-efficiency phosphate solubilizing bacteria is CGMCC NO. 12767;
the method for preparing the high-efficiency phosphate fertilizer comprises the following steps:
step 1: fermenting by using efficient phosphate solubilizing bacteria with the preservation number of CGMCC NO.12767 to obtain fermentation liquor;
step 2: preparing half-cooked grains from wheat, corn grit and/or rice;
step 3: inoculating 10% of the fermentation liquid into sterilized half-cooked grains, culturing at 25 deg.C for 5 days, turning over grains after 5 days, and standing for 10 days for 15 days;
step 4: culturing for 15 days, drying, pulverizing, and sieving to obtain fungal spore powder;
step 5: mixing the fungal spore powder with grass peat, light calcium carbonate and a phosphorus source which are sterilized at high temperature, wherein the weight ratio of the grass peat to the light calcium carbonate to the phosphorus source is 1000:4000:10:100, stirring evenly and crushing to obtain the high-efficiency phosphate fertilizer, wherein the phosphorus source is FePO4、CaHPO4、Ca3(PO4)2、CaH2PO4MPR, lecithin, AlPO4Or fluorapatite;
in Step1, the fermentation process is as follows:
(1) inoculating the penicillium strain with the preservation number of CGMCC NO.12767 to the slant of an MEA culture medium, and culturing at 28 ℃ for 7 days to obtain slant seeds;
(2) inoculating the slant seeds into a liquid culture medium, wherein the liquid culture medium comprises the following components: 30g of cane sugar, 30g of soybean meal and FeSO4·7H2O0.005g+MnSO40.008g+H3BO30.002g+ZnSO40.005g+CuSO40.004g+Na2MoO40.004g+CoCl20.002g and 0.04g inositol, adding distilled water to 1L, culturing at 29 deg.C with initial pH of 5-6 to obtain liquid seed;
(3) inoculating liquid seeds into a fermentation tank, wherein the fermentation medium comprises the following components: 30g of cane sugar, 30g of soybean meal and FeSO4·7H200.005g+MnSO40.008g+H3BO30.002g+ZnSO40.005g+CuSO40.004g+Na2MoO40.004g+CoCl20.002g + inositol 0.04g, adding distilled water to 1L, the initial pH value is 5-6, and the culture conditions are as follows: fermenting at 26-32 deg.C and stirring speed of 150-250rpm for 4-6 days to obtain fermentation liquid.
2. Use according to claim 1, wherein the process of making parboiled grain at Step2 comprises:
soaking wheat, corn grit and/or rice in warm water at 55 deg.C for a period of time until the water content of the grain is 30%, and aging at 100 deg.C for 10 min to obtain half-cooked grain.
3. The application of the high-efficiency phosphate solubilizing bacteria in the preparation of the microbial compound seed coating agent is characterized in that the preservation number of the high-efficiency phosphate solubilizing bacteria is CGMCC NO. 12767;
the method for preparing the microbial compound seed coating agent comprises the following steps:
step 1: fermenting by using efficient phosphate solubilizing bacteria with the preservation number of CGMCC NO.12767 to obtain fermentation liquor;
step 2: preparing half-cooked grains from wheat, corn grit and/or rice;
step 3: inoculating 10% of the fermentation liquid into sterilized half-cooked grains, culturing at 25 deg.C for 5 days, turning over grains after 5 days, and standing for 10 days for 15 days;
step 4: culturing for 15 days, drying, pulverizing, and sieving to obtain fungal spore powder;
step 5: adding the film forming agent into warm water at 50 ℃, stirring until the film forming agent is completely dissolved, continuously adding the insecticide, the dispersing agent, the protective agent, the coloring agent and the nutritional agent into the warm water, uniformly mixing, then grinding for 4 hours in a sand mill to prepare a suspending agent, finally adding fungal spore powder into the suspending agent, and uniformly mixing to obtain the microbial compound seed coating agent;
in Step1, the fermentation process is as follows:
(1) inoculating the penicillium strain with the preservation number of CGMCC NO.12767 to the slant of an MEA culture medium, and culturing at 28 ℃ for 7 days to obtain slant seeds;
(2) inoculating the slant seeds into a liquid culture medium, wherein the liquid culture medium comprises the following components: 30g of cane sugar, 30g of soybean meal and FeSO4·7H2O0.005g+MnSO40.008g+H3BO30.002g+ZnSO40.005g+CuSO40.004g+Na2MoO40.004g+CoCl20.002g and 0.04g inositol, adding distilled water to 1L, culturing at 29 deg.C with initial pH of 5-6 to obtain liquid seed;
(3) inoculating liquid seeds into a fermentation tank, wherein the fermentation medium comprises the following components: 30g of cane sugar, 30g of soybean meal and FeSO4·7H200.005g+MnSO40.008g+H3BO30.002g+ZnSO40.005g+CuSO40.004g+Na2MoO40.004g+CoCl20.002g + inositol 0.04g, adding distilled water to 1L, the initial pH value is 5-6, and the culture conditions are as follows: fermenting at 26-32 deg.C and stirring speed of 150-250rpm for 4-6 days to obtain fermentation liquid.
4. Use according to claim 3, wherein the process of making parboiled grain at Step2 comprises:
soaking wheat, corn grit and/or rice in warm water at 55 deg.C for a period of time until the water content of the grain is 30%, and aging at 100 deg.C for 10 min to obtain half-cooked grain.
5. The application of claim 3, wherein in Step5, the dosage of each raw material for preparing the microbial compound seed coating agent is as follows by mass percent:
6. the use as claimed in claim 5, wherein the pesticide is any one or a mixture of any more of metalaxyl-M, yidamide, thiram, iprodione, imidacloprid, triticonazole, cypermethrin, tebuconazole, chlorbenzuron and flutriafol.
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| CN101444227A (en) * | 2009-01-09 | 2009-06-03 | 北京理工大学 | Multifunctional biological seed coating agent and preparation method thereof |
| CN103695318A (en) * | 2013-12-20 | 2014-04-02 | 云南金雨庄生物科技有限责任公司 | Phosphorus-dissolving fungi and method for preparing biological phosphate fertilizer through fermenting edible fungi residues by phosphorus-dissolving fungi |
| CN104797701A (en) * | 2012-11-16 | 2015-07-22 | 诺维信生物农业公司 | Microbial strains, compositions, and methods for increasing available phosphate for plants |
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| CN101444227A (en) * | 2009-01-09 | 2009-06-03 | 北京理工大学 | Multifunctional biological seed coating agent and preparation method thereof |
| CN104797701A (en) * | 2012-11-16 | 2015-07-22 | 诺维信生物农业公司 | Microbial strains, compositions, and methods for increasing available phosphate for plants |
| CN103695318A (en) * | 2013-12-20 | 2014-04-02 | 云南金雨庄生物科技有限责任公司 | Phosphorus-dissolving fungi and method for preparing biological phosphate fertilizer through fermenting edible fungi residues by phosphorus-dissolving fungi |
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