CN107523508A - With potassium decomposing, the aspergillus niger JXJ A01 of phosphorus decomposing and degrading organic phosphor pesticides activity and its application - Google Patents
With potassium decomposing, the aspergillus niger JXJ A01 of phosphorus decomposing and degrading organic phosphor pesticides activity and its application Download PDFInfo
- Publication number
- CN107523508A CN107523508A CN201710843897.8A CN201710843897A CN107523508A CN 107523508 A CN107523508 A CN 107523508A CN 201710843897 A CN201710843897 A CN 201710843897A CN 107523508 A CN107523508 A CN 107523508A
- Authority
- CN
- China
- Prior art keywords
- aspergillus niger
- potassium
- decomposing
- jxj
- jxja01
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/66—Aspergillus
- C12R2001/685—Aspergillus niger
-
- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D3/00—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances
- A62D3/02—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances by biological methods, i.e. processes using enzymes or microorganisms
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/45—Form not covered by groups C05G5/10 - C05G5/18, C05G5/20 - C05G5/27, C05G5/30 - C05G5/38 or C05G5/40, e.g. soluble or permeable packaging
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
-
- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
- A62D2101/20—Organic substances
- A62D2101/26—Organic substances containing nitrogen or phosphorus
-
- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
- A62D2101/40—Inorganic substances
-
- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
- A62D2101/40—Inorganic substances
- A62D2101/45—Inorganic substances containing nitrogen or phosphorus
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2101/00—Agricultural use
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Mycology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Botany (AREA)
- Molecular Biology (AREA)
- Toxicology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Business, Economics & Management (AREA)
- Emergency Management (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Soil Sciences (AREA)
- Materials Engineering (AREA)
- Pest Control & Pesticides (AREA)
- Fertilizers (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to technical field of microbe application, more particularly to a kind of aspergillus niger JXJ A01 with potassium decomposing, phosphorus decomposing and degrading organic phosphor pesticides activity, aspergillus niger JXJ A01 are further related in dissolving slightly solubility potassium, organophosphor and Phos, the application of degrading organic phosphor pesticides, further relate to applications of the aspergillus niger JXJ A01 in terms of microbial manure, aspergillus niger JXJ A01 are preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preserving number CGMCC No.13775.Aspergillus niger JXJ A01 are respectively provided with very strong dissolution to insoluble phosphorus and potassium breeze, it is allowed to be converted into the soluble phosphorus ＆ potassium element of plant available, and being capable of degrading organic phosphor pesticides omethoate, it is allowed to form nontoxic titanium pigment acid group, reduce pesticide concentration, increase soil soluble P elements, the bacterium can be applied to increase the effective potassium of soil soluble and phosphorus, repair pollution of the organophosphorus pesticide to soil.
Description
Technical field
The present invention relates to technical field of microbe application, and in particular to one kind has slightly solubility potassium, organophosphor and inorganic
Phosphorus is converted into applications of the aspergillus niger JXJ A01 of soluble potassium and phosphorus in terms of microbial manure, and is related to and has with degraded
The aspergillus niger JXJ A01 of machine phosphorus insecticide answering in terms of farmland organophosphorus pesticide, improvement and reparation agricultural land soil is reduced
With.
Background technology
Phosphorus element is that plant normal growth develops indispensable indispensable element.More than 95% P elements are plant in soil
It is difficult to the insoluble phosphorus directly absorbed, the farmland arable soil in China about 74% lacks phosphorus, is using organic and chemical phosphatic ferfilizer
The main source of soil phophorus.But soil mineral confrontation P elements have very strong reactivity, tend to be formed slightly solubility without
Phosphate is imitated, the phosphate fertilizer this season utilization rate for causing to apply only has 5%~25%.Phosphate fertilizer is essentially from ground phosphate rock, but ground phosphate rock is
Non-renewable resources, will be complete in in-fighting in 50~100 years, therefore, how increase titanium pigment constituent content in soil, improve phosphorus
The problem of fertile utilization ratio is agriculture urgent need to resolve.
One of a great number of elements necessary to potassium is growth and development of plants.Under general working condition, crop needs potassium amount and nitrogen phase
When.Although containing a large amount of potassium elements (occupying the 4th in all mineral nutrient elements of the earth's crust) in soil, more than 90% is plant
The invalid potassium that can not be absorbed.Although China's insoluble potassic mineral aboundresources, soluble potassium ore resources are relatively deficient, 50-
70% dosage depends on import.Farmland wanes effective potassium as one of limiting factor of China's agricultural production sustainable development.Cause
How this, by insoluble invalid potassium in soil be changed into soluble effective potassium, China's soluble potassium ore resources are deficient alleviating
It is weary, and promote agricultural sustainable development aspect significant.Many microbial soluble solution potassium ores, it is insoluble invalid to make
Potassium is changed into soluble effective potassium, and research at present is mostly potassium bacterium, filamentary mould then less report.Mould because mycelia is flourishing,
Solid fermentation is carried out using various agricultural wastes, growth is rapid, and spore output is high, is adapted to quickly produce microorganism
Solid fertilizer.
In the past few decades, China's agricultural causes soil pollution tight characterized by using a large amount of chemical fertilizer and organophosphorus pesticide
Weight, particularly persticide residue are high, have a strong impact on the quality of crops and the development of modern material reserve.Microorganism is to many
Noxious material all has very strong passivation and degradation, and it is insoluble cadmium to make the passivation of the harmful heavy metals such as soluble cadmium and lead
And lead, reduce crop and absorb, the noxious materials such as agricultural chemicals are degraded to innocuous substance, or even be degraded to beneficial to plant growth
Nutritional ingredient.Therefore, apply related microorganisms in soil, can effectively reduce heavy metal pollution, and agricultural chemicals is residual in soil
Allowance and residual life, improve soil quality, improve the quality of crops, promote the development of the ecological agriculture.
The content of the invention
The purpose of the present invention is to be the needs for modern material reserve sustainable development, there is provided one kind can efficiently drop
Solving slightly solubility potassium, organophosphor and Phos can incite somebody to action for aspergillus niger JXJ A01, the aspergillus niger JXJ A01 of soluble potassium and phosphorus
Organic phosphorus pesticide degradation is nontoxic orthophosphates, eliminates the residual of organophosphorus pesticide in soil, and provide and can absorb for plant
Available phosphorus.
The purpose of the present invention is achieved through the following technical solutions：
A kind of aspergillus niger JXJ A01 with potassium decomposing, phosphorus decomposing and degrading organic phosphor pesticides activity, are preserved in China Microbiological
Culture presevation administration committee common micro-organisms center, preserving number CGMCC No.13775, preservation date are 04 month 2017
20 days.Preservation organization address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica, postcode：
100101, phone：86-10-64807355.
Aspergillus JXJ A01 of the present invention produce the radial conidial head of brown-black, and conidiophore is born in ball
In shape top capsule, bunchiness black spherical conidium is born on conidiophore, the typical morphological feature phase with aspergillus niger
Symbol.Bacterial strain JXJ A01 well-growns on various agricultural discarded object culture medium, and produce a large amount of black spores；Spore can be
Germination and growth under the conditions of 15-47 DEG C, optimal germination and growth temperature are 28-37 DEG C；Spore handles 30 under 65 DEG C of wet heat condition
Remain able to sprout after min；Spore under conditions of pH2-12 can germination and growth, most suitable germination and growth pH value is that 4-8 is left
It is right；Spore and mycelium have certain tolerance and degradation to organophosphorus pesticide (such as omethoate).Through with
ITS sequence and 18S rRNA Gene sequence comparison analyses in GenBank databases find, the bacterium ITS sequence fragment (591
Bp) with the similitude of aspergillus niger up to 100%, 18S rRNA gene orders (1709bp) and the similitude of aspergillus niger be 99%, knot
Close and state morphological feature, bacterial strain JXJ A01 are accredited as aspergillus niger (Aspergillus niger), are named as aspergillus niger JXJ
A01, the accession number of the 18S rRNA gene orders and ITS sequence of the bacterial strain in GenBank be respectively MF145518 and
MF543023, specifically see SEQ ID NO:1 and SEQ ID NO:2.
Aspergillus niger JXJ A01 provided by the invention with potassium decomposing, phosphorus decomposing and degrading organic phosphor pesticides activity, using China
Tetraphenyl borate potassium gravimetric method detection in people's republic's standard GB/T/T 8574-2010, in laboratory conditions, inoculation
The bacterium spore and mycelium and after cultivating 10d, they discharge the amount of soluble potassium respectively than blank pair from refractory potassic mine powder
According to the high 158.08mgL of group-1And 261.16mgL-1。
Therefore, the invention further relates to the above-mentioned aspergillus niger JXJ A01 with potassium decomposing, phosphorus decomposing and degrading organic phosphor pesticides activity
In the application for the soluble potassium that slightly solubility potassium in soil is converted into plant available.
Aspergillus niger JXJ A01 provided by the invention with potassium decomposing, phosphorus decomposing and degrading organic phosphor pesticides activity, according to China
Ministry of Agriculture professional standard NY/T 1847-2010 method detection, inoculating spores and after cultivating 4-10d, calcium phosphate (20g/L),
Magnesium phosphate (20g/L), aluminum phosphate (10g/L), ferric phosphate (10g/L), phytic acid calcium (20g/L), lecithin (2 g/L) and ground phosphate rock
(10g/L, P2O5Content be about 25%) in the nutrient solution of culture medium titanium pigment element highest content respectively than not connecing pair of bacterium
According to high 3784mg/L, 4698mg/L, 813mg/L, 168mg/L, 3082mg/L, 81mg/L, 509mg/L is organized, to this 7 kinds of phosphorus sources
Degradation rate be respectively 94.66%, 100%, 32.72%, 15.42%, 59.68%, 100%, 46.61%.
Therefore, the invention further relates to the above-mentioned aspergillus niger JXJ A01 with potassium decomposing, phosphorus decomposing and degrading organic phosphor pesticides activity
In the application for the titanium pigment that each slightly solubility organophosphor and Phos in soil are converted into plant available.
It is provided by the invention with potassium decomposing, phosphorus decomposing and degrading organic phosphor pesticides activity aspergillus niger JXJ A01, its spore and
Mycelium is inoculated in the 40% missible oil of omethoate phosphorus decomposing culture medium that volumetric concentration is 0.1%, titanium pigment in nutrient solution after 5d
Hydrochlorate concentration is higher 11.77mgL than control group respectively-1And 6.79mgL-1。
Therefore, the invention further relates to the above-mentioned aspergillus niger JXJ A01 with potassium decomposing, phosphorus decomposing and degrading organic phosphor pesticides activity
The organophosphorus pesticide in soil is eliminated, improvement and the application for repairing organophosphorus pesticide pollution soil.
Aspergillus niger JXJ A01 provided by the invention with potassium decomposing, phosphorus decomposing and degrading organic phosphor pesticides activity, by bacterial strain
JXJ A01 are inoculated with sterile wheat bran and cultivated, and after a large amount of black spores to be formed, add feed, continuous to feed kunming mice 4
In week, mouse growth is normal, does not occur the phenomena of mortality, illustrate the bacterium and its metabolite do not have to animals such as mouse it is pathogenic or
Toxicity.
The present invention also provides the preparation side of two kinds of aspergillus niger JXJ A01 solid multifunctionals bacterial manure and soil improvement renovation agent
Method.
Method one：Aspergillus niger JXJ A01 spore inoculating is trained in sterile PDA liquid medium or other sterile liquids
Support base (waste liquid of high BOD values etc. caused by including food processing factory etc.), 28 DEG C, under conditions of 160rpm after culture 1-2d,
Sprinkling be inoculated into handled through high temperature stacking fermentation (or high-temp steam sterilizing processing) various agricultural wastes it is (or discarded edible
Bacterium mushroom bran etc.), fowl and animal excrement, the mixture of ground phosphate rock and potassium breeze, in 30 DEG C or so, the condition of relative humidity 85%~90%
Lower culture 3-6d, after growing a large amount of black spores, after being dried by the methods of natural draft drying or hot-air drying, according to
Plant growth needs, and adds appropriate trace element, and particle is made in well mixed i.e. available comminutor, that is, obtains containing largely may be used
The mineral matter elements such as dissolubility phosphorus potassium, abundant N and organic matter, highdensity aspergillus niger are lived the efficient, environmentally friendly multi-functional of spore
Aspergillus niger bacterial manure.
Method two：By various agricultural wastes (or discarded edible mushroom mushroom bran etc.), fowl and animal excrement, ground phosphate rock and potassium breeze etc.
Solid mixture (mass ratio 60:20:10:10) aspergillus niger JXJ A01 liquid spawns are accessed, and accumulate hot fermentation 5-10
D, then dry or natural air drying or hot-air are dried, it is standby.By aspergillus niger JXJ A01 spore inoculating in sterile PDA liquid
Culture medium or other aseptic liquid nutrient mediums (waste liquid of high BOD values etc. caused by including food processing factory etc.), 28 DEG C,
After cultivating 2d under conditions of 160rpm, sprinkling is inoculated into sterile wheat bran and stalk mixing (mass ratio 1:1) solid medium
On, 3-6d is cultivated, after a large amount of black spores are grown, according to certain ratio, the drying solid with accumulation hot fermentation processing
Mixture is well mixed, and is recycled comminutor to be granulated mixture, that is, is obtained containing mineral matters such as great amount of soluble phosphorus potassium
Efficient, the environmentally friendly multi-functional aspergillus niger bacterial manure of element, abundant N and organic matter, highdensity aspergillus niger work spore.
The technique effect of the present invention：Aspergillus niger JXJ A01 are nontoxic, have and turn slightly solubility potassium, organophosphor and Phos
Soluble potassium and phosphorus effect are turned to, farmland organophosphorus pesticide, improvement and reparation agricultural land soil can be reduced, can configure black song
Mould JXJ A01 solid multifunctionals bacterial manure and soil improvement renovation agent, as fertilizer and soil-repairing agent.
Figure of description
The Soluble phosphorus circle that Fig. 1 bacterial strain JXJ A01 are formed on calcium phosphate and phytic acid calcium flat board.
Degradation capabilities of Fig. 2 bacterial strain JXJ A01 to slightly solubility solution potassium ore.
After Fig. 3 inoculating strain JXJ A01 mycelium in organophosphorus pesticide omethoate culture medium titanium pigment content change.
Wherein, a is that 40% missible oil of omethoate content is 0.1% (v/v)；B is that 40% missible oil of omethoate content is 0.2% (v/v)；c
It is 0.3% (v/v) for 40% missible oil of omethoate content；D is that 40% missible oil of omethoate content is 0.4% (v/v)；E is 40% oxygen
Dimethoate emulsifiable concentrate content is 0.5% (v/v).
After Fig. 4 inoculating strain JXJ A01 spores in organophosphorus pesticide omethoate culture medium titanium pigment content change.Its
In, a is that 40% missible oil of omethoate content is 0.1% (v/v)；B is that 40% missible oil of omethoate content is 0.2% (v/v).
Embodiment
The screening of 1 phosphorus decomposing aspergillus
Solid plate screens：The forest soil for picking up from Jiujiang City of Jiangxi Province is subjected to gradient dilution with sterilized water, it is appropriate to take
The dilution 0.2mL of gradient, it is coated on the Ministry of Agriculture professional standard NY/T 1847-2010 phosphorus decomposing solid medium
(glucose 10g, (NH4)2SO40.5g, NaCl 0.3g, MgSO4·7H2O 0.3g, KCl 0.3g, FeSO4·4H2O 0.036
G, MnSO4·4H2O 0.03g, calcium phosphate or phytic acid calcium 10g, agar 18g, distilled water 1000mL, pH7.0), and in 28 DEG C of trainings
Support after cultivating 1-7d in case, observe the growing state of bacterium, and whether each periphery of bacterial colonies phosphorus decomposing circle occurs, selects phosphorus decomposing circle obvious
Bacterial strain JXJ A01 (Fig. 1) carry out follow-up study.
2 aspergillus niger JXJ A01 identification
The methods of being analyzed according to cultural characteristic, morphological feature, 18S rRNA gene orders and ITS sequence, to Decomposing phosphate activity
Very strong mould JXJ A01 are identified.The bacterium mycelia is flourishing, branch is more, and initial stage is canescence, after be changed into brown-black, and produce
The radial conidial head of raw brown-black, conidiophore are born in spherical top capsule, and bunchiness black spherical conidium raw
In on conidiophore, these belong to the typical morphological feature of aspergillus niger.Through with the ITS sequences in GenBank databases
Found with 18S rRNA Gene sequence comparison analyses, the bacterium ITS sequence fragment (591bp) and the similitude of aspergillus niger reach
The similitude of 100%, 18S rRNA gene orders (1709bp) and aspergillus niger is 99%, comprehensive morphological feature, ITS sequence and
18S rRNA gene sequencing results, bacterial strain JXJ A01 are accredited as aspergillus (Aspergillus sp.), are named as black song
Mould JXJ A01, the accession number of the 18S rRNA gene orders and ITS sequence of the bacterial strain in GenBank databases are respectively
MF145518 and MF543023, specifically see SEQ ID NO：1 and SEQ ID NO：2.The bacterium has been preserved in China Microbiological bacterium
Kind preservation administration committee common micro-organisms center, preserving number CGMCC No.13775, preservation date are 04 month 2017 20
Day.Preservation organization address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica, postcode：
100101, phone：86-10-64807355.
3 aspergillus niger JXJ A01 potassium decomposing activity
By bacterial strain JXJ A01 spore suspensions or mycelium access liquid potassium decomposing culture medium (10g glucose, 0.3g NaCl, 1
g NaNO3, 0.5g Na2HPO4, 0.5g MgSO4·7H2O, 0.01g FeSO4·7H2O, potassium ore powder 10g, 1000 mL steaming
Distilled water, pH7.4~7.6,121 DEG C of sterilizing 30min) in, 28 DEG C, cultivate under the conditions of 160rpm, per 2d, once, sample exists for sampling
Centrifuged under the conditions of 4500rpm, using the tetraphenyl borate potassium weight in National Standard of the People's Republic of China GB/T 8574-2010
Soluble potassium constituent content in method measure supernatant.With the extension of incubation time, the potassium breeze in culture medium gradually disappears
Lose, soluble potassium constituent content increases sharply in nutrient solution, the solubility accessed after 10d in spore and mycelial nutrient solution
Determination of Potassium (158.08 ± 12.43) mgL higher than control group respectively-1(261.16 ± 20.53) mgL-1(Fig. 2),
The pH of nutrient solution drops to 2.4 or so simultaneously, and therefore, the bacterium has very strong potassium decomposing activity, and it is its potassium decomposing to produce organic acid
One of mechanism.
4 aspergillus niger JXJ A01 Decomposing phosphate activity
By aspergillus niger JXJ A01 spore suspensions access liquid phosphorus decomposing culture medium (glucose 10g, (NH4)2SO40.5g, NaCl
0.3g, MgSO4·7H2O 0.3g, KCl 0.3g, FeSO4·4H2O 0.036g, MnSO4·4H2O 0.03g, distilled water
1000mL, phosphorus source is added after adjusting pH7.0, wherein ferric phosphate, aluminum phosphate and ground phosphate rock is 10g/L, lecithin 2g) or (Portugal
Grape sugar 20g, (NH4)2SO41g, NaCl 0.6g, MgSO4·7H2O 0.6g, KCl 0.6g, FeSO4·4H2O 0.072g,
MnSO4·4H2O 0.06g, distilled water 1000mL, phosphorus source is added after adjusting pH7.0, calcium phosphate, phytic acid calcium and magnesium phosphate are
In 20g/L), it is 10 to make spore ultimate density5~106CFU·mL-1, cultivate under the conditions of 28 DEG C, 160rpm, sampled per 24h
Once, sample centrifuges under the conditions of 4500rpm, supernatant according to the Ministry of Agriculture professional standard NY/T 1847-2010 side
Method detects the concentration of its soluble effectively P elements, is reference zeroing using deionized water, using do not connect the blank cultures of bacterium as
Control group.As a result find, after cultivating 4-10d, in calcium phosphate, magnesium phosphate, aluminum phosphate, ferric phosphate, phytic acid calcium, lecithin and phosphorus
In miberal powder nutrient solution titanium pigment element highest content respectively than do not connect the control group of bacterium it is high 3784mg/L, 4683.2mg/L,
813mg/L, 168mg/L, 3082mg/L, 94mg/L and 512mg/L (table 1), the degradation rate to this 7 kinds of phosphorus sources are respectively
94.66%th, 100%, 32.72%, 15.42%, 59.68%, 100%, 47.23%, while the pH of nutrient solution declines (table 1),
Illustrate that it is one of phosphorus decomposing mechanism of the bacterium to produce organic acid.
Titanium pigment constituent content (mgL in nutrient solution after the access aspergillus niger spore culture of table 1-1) and pH changes
Tolerances and degradation effect of the 5 aspergillus niger JXJ A01 to organophosphorus pesticide
Phosphorus source in the Ministry of Agriculture professional standard NY/T 1847-2010 phosphorus decomposing culture medium prescription is removed, after sterilizing
0.1%~0.5% (v/v) 40% missible oil of omethoate is added, mycelium or spore are inoculated with, under conditions of 28 DEG C, 160rpm
Culture, per 24h, sampling once, observes mycelial growth situation and spore germination situation, sample centrifuges under the conditions of 4500rpm,
Supernatant detects the concentration of its soluble effectively P elements according to the Ministry of Agriculture professional standard NY/T 1847-2010 method,
Make reference zeroing not connect the blank cultures of bacterium, as a result find, aspergillus niger JXJ A01 mycelium is containing 0.3% (v/v)
40% missible oil of omethoate phosphorus decomposing culture medium in can survive, although containing 0.4%~0.5% (v/v) 40% omethoate breast
Mycelium is dead in oily phosphorus decomposing culture medium, but omethoate degradation of pesticide can be still titanium pigment (figure by the mycelium accessed
3), the enzyme containing degraded omethoate in this explanation aspergillus niger JXJ A01 mycelium；Aspergillus niger JXJ A01 spore containing
It can not be sprouted in 0.3% (v/v) 40% missible oil of omethoate phosphorus decomposing culture medium, but 40% missible oil of omethoate volumetric concentration is reduced to
When 0.2%, the spore 4d of the bacterium can sprout, when 40% missible oil of omethoate volumetric concentration is reduced to 0.1%, to spore
Germination inhibitor very little, spore germination and form a large amount of mycelium after 1d, start to play degraded oxygen pleasure after spore germination
The effect of fruit, cause the titanium pigment content in omethoate culture medium to increase sharply (Fig. 4), and shape is grown after spore germination
Into mycelium it is stronger to the degradation of omethoate.
Toxicity of the 6 aspergillus niger JXJ A01 to kunming mice
Aspergillus niger JXJ A01 nutrient solutions are inoculated in sterile wheat bran and cultivated, treat that mycelial growth forms a large amount of black spores
Afterwards according still further to 1:2 (mass ratio, cultures:Feed) feed is mixed into, kunming mice is continuously fed with mixture 4 weeks, mouse growth
Normally, illustrate that bacterium mycelium, spore and its metabolite do not have toxicity to animals such as mouse.
The production of 7 aspergillus niger JXJ A01 bacterial manure
Method one：By dry various agricultural wastes (or discarded edible mushroom mushroom bran etc.), fowl and animal excrement, ground phosphate rock and potassium
The solids such as miberal powder, it is 60 according to mass ratio:20:10:10 are mixed, and carry out accumulating hot fermentation processing 5-10 after well mixed
D or high-temp steam sterilizing processing, it is standby.By aspergillus niger JXJ A01 spore inoculating in sterile PDA liquid medium or its
Its aseptic liquid nutrient medium (waste liquid of high BOD values etc. caused by including food processing factory etc.), 28 DEG C, under conditions of 160rpm
After cultivating 2d, sprinkling is inoculated on above-mentioned treated solid mixed culture medium (solid medium thickness about 10-15 cm), in
30 DEG C or so, cultivate 3-6d under the conditions of relative humidity 85%~90%, after growing a large amount of black spores, by gravity-flow ventilation or
After the methods of hot-air is dried, according to plant growth needs, add appropriate boron, selenium and other trace elements, it is well mixed after with making
Particle is made in grain machine, that is, obtains containing the mineral matter elements such as great amount of soluble phosphorus potassium, abundant N and organic matter, highdensity black
Efficient, the environmentally friendly multi-functional aspergillus niger bacterial manure of aspergillus work spore.
Method two：By various agricultural wastes (or discarded edible mushroom mushroom bran etc.), fowl and animal excrement, ground phosphate rock and potassium breeze etc.
Solid mixture (mass ratio 60:20:10:10) aspergillus niger JXJ A01 liquid spawns are accessed, and accumulate hot fermentation 5-10
D, then dry or natural air drying or hot-air drying to water content are about 10%-15% or so, it is standby.By aspergillus niger JXJ A01
Spore inoculating it is (caused by including food processing factory etc. high in sterile PDA liquid medium or other aseptic liquid nutrient mediums
Waste liquid of BOD values etc.), 28 DEG C, cultivate 2d under conditions of 160rpm after, sprinkling is inoculated into sterile wheat bran and stalk mixing (matter
Amount is than being 1:1) on solid medium, 3-6d is cultivated, after a large amount of black spores are grown, according to certain ratio, is sent out with accumulation
Ferment and the agricultural wastes of drying (or discarded edible mushroom mushroom bran etc.), fowl and animal excrement, the solids bulk hair of ground phosphate rock and potassium breeze
Ferment thing mixes, and the aspergillus niger work spore count for adjusting mixture reaches 0.2 hundred million/more than g, recycles comminutor to enter mixture
Row is granulated, that is, is obtained containing the mineral matter elements such as great amount of soluble phosphorus potassium, abundant N and organic matter, the work of highdensity aspergillus niger
Efficient, the environmentally friendly multi-functional aspergillus niger bacterial manure of spore.
Sequence table
<110>Zhang Ping Huo
<120>With potassium decomposing, the aspergillus niger JXJ A01 of phosphorus decomposing and degrading organic phosphor pesticides activity and its application
<160> 2
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1709
<212> DNA
<213>Aspergillus niger (Aspergillus niger)
<400> 1
agaaagcatg tctagtataa gcactttata ctgtgaaact gcgaatgggc tcattaaatc 60
agttatcgtt tatttgatag taccttacta catggatacc tgtggtaatt ctagagctaa 120
tacatgctga aaacctcgac ttcggaaggg gtgtatttat tagataaaaa accaatgccc 180
ttcggggctc cttggtgaat cataataact taacgaatcg catggccttg cgccggcgat 240
ggttcattca aatttctgcc ctatcaactt tcgatggtag gatagtggcc taccatggtg 300
gcaacgggta acggggaatt agggttcgat tccggagagg gagcctgaga aacggctacc 360
acatccaagg aaggcagcag gcgcgcaaat tacccaatcc cgacacgggg aggtagtgac 420
aataaatact gatacggggc tcttttgggt ctcgtaattg gaatgagtac aatctaaatc 480
ccttaacgag gaacaattgg agggcaagtc tggtgccagc agccgcggta attccagctc 540
caatagcgta tattaaagtt gttgcagtta aaaagctcgt agttgaacct tgggtctggc 600
tggccggtcc gcctcaccgc gagtactggt ccggctggac ctttccttct ggggaatctc 660
atggccttca ctggctgtgg ggggaaccag gacttttact gtgaaaaaat tagagtgttc 720
aaagcaggcc tttgctcgaa tacattagca tggaataata gaataggacg tgcggttcta 780
ttttgttggt ttctaggacc gccgtaatga ttaataggga tagtcggggg cgtcagtatt 840
cagctgtcag aggtgaaatt cttggatttg ctgaagacta actactgcga aagcattcgc 900
caaggatgtt ttcattaatc agggaacgaa agttagggga tcgaagacga tcagataccg 960
tcgtagtctt aaccataaac tatgccgact agggatcgga cggtgtttct attatgaccc 1020
gttcggcacc ttacgagaaa tcaaagtttt tgggttctgg ggggagtatg gtcgcaaggc 1080
tgaaacttaa agaaattgac ggaagggcac caccaggcgt ggagcctgcg gcttaatttg 1140
actcaacacg gggaaactca ccaggtccag acaaaataag gattgacaga ttgagagctc 1200
tttcttgatc ttttggatgg tggtgcatgg ccgttcttag ttggtggagt gatttgtctg 1260
cttaattgcg ataacgaacg agacctcggc ccttaaatag cccggtccgc atttgcgggc 1320
cgctggcttc ttagggggac tatcggctca agccgatgga agtgcgcggc aataacaggt 1380
ctgtgatgcc cttagatgtt ctgggccgca cgcgcgctac actgacaggg ccagcgagta 1440
catcaccttg gccgagaggt ctgggtaatc ttgttaaacc ctgtcgtgct ggggatagag 1500
cattgcaatt attgctcttc aacgaggaat gcctagtagg cacgagtcat cagctcgtgc 1560
cgattacgtc cctgcccttt gtacacaccg cccgtcgcta ctaccgattg aatggctcgg 1620
tgaggccttc ggactggctc aggagggttg gcaacgaccc cccagagccg gaaagttggt 1680
caaacccggt cattagagaa gtaagttga 1709
<210> 2
<211> 591
<212> DNA
<213>Aspergillus niger (Aspergillus niger)
<400> 2
tgaacctgcg gaaggatcat taccgagtgc gggtcctttg ggcccaacct cccatccgtg 60
tctattatac cctgttgctt cggcgggccc gccgcttgtc ggccgccggg ggggcgcctt 120
tgccccccgg gcccgtgccc gccggagacc ccaacacgaa cactgtctga aagcgtgcag 180
tctgagttga ttgaatgcaa tcagttaaaa ctttcaacaa tggatctctt ggttccggca 240
tcgatgaaga acgcagcgaa atgcgataac taatgtgaat tgcagaattc agtgaatcat 300
cgagtctttg aacgcacatt gcgccccctg gtattccggg gggcatgcct gtccgagcgt 360
cattgctgcc ctcaagcccg gcttgtgtgt tgggtcgccg tccccctctc cggggggacg 420
ggcccgaaag gcagcggcgg caccgcgtcc gatcctcgag cgtatggggc tttgtcacat 480
gctctgtagg attggccggc gcctgccgac gttttccaac cattttttcc aggttgacct 540
cggatcaggt agggataccc gctgaactta agcatatcaa taagcggagg a 591
Claims (9)
1. a kind of aspergillus niger JXJA01 with potassium decomposing, phosphorus decomposing and degrading organic phosphor pesticides activity, it is characterized in that, the aspergillus niger
JXJA01 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preserving number CGMCC No.13775,
Preservation date is on 04 20th, 2017.
2. the application of the aspergillus niger JXJA01 as claimed in claim 1 with potassium decomposing, phosphorus decomposing and degrading organic phosphor pesticides activity,
It is characterized in that：For slightly solubility potassium to be converted into soluble potassium, plant is promoted to absorb potassium element.
3. the application of the aspergillus niger JXJA01 as claimed in claim 1 with potassium decomposing, phosphorus decomposing and degrading organic phosphor pesticides activity,
It is characterized in that：For slightly solubility organophosphor to be converted into titanium pigment, plant is promoted to absorb P elements.
4. the application of the aspergillus niger JXJA01 as claimed in claim 1 with potassium decomposing, phosphorus decomposing and degrading organic phosphor pesticides activity,
Characterized in that, for various slightly solubility Phos to be converted into titanium pigment, plant is promoted to absorb P elements.
5. the application of the aspergillus niger JXJA01 as claimed in claim 1 with potassium decomposing, phosphorus decomposing and degrading organic phosphor pesticides activity,
It is characterized in that：It is nontoxic soluble phosphate for degrading organic phosphor pesticides, reduces Pesticide-Polluted Soil, improvement and reparation
The pollution by pesticides of soil.
A kind of 6. preparation method of aspergillus niger JXJA01 solid multifunctionals bacterial manure and soil improvement renovation agent, it is characterised in that：Will
Aspergillus niger JXJA01 spore inoculating described in claim 1 in sterile PDA liquid medium or other aseptic liquid nutrient mediums,
28 DEG C, cultivate 1-2d under conditions of 160rpm after, sprinkling is inoculated into be handled or high-temp steam sterilizing processing through high temperature stacking fermentation
Various agricultural wastes, fowl and animal excrement, the mixture of ground phosphate rock and potassium breeze, in 30 DEG C or so, relative humidity 85%~
3-6d is cultivated under the conditions of 90%, after growing a large amount of black spores, is dried, according to plant growth needs, adds appropriate trace element,
Particle is made in well mixed i.e. available comminutor, that is, obtains containing great amount of soluble potassium element, P elements and aspergillus niger work spore
Multi-functional aspergillus niger bacterial manure.
A kind of 7. preparation method of aspergillus niger JXJA01 solid multifunctionals bacterial manure and soil improvement renovation agent, it is characterised in that：Will
Various agricultural wastes, fowl and animal excrement, the solid mixture access aspergillus niger JXJA01 liquid spawns of ground phosphate rock and potassium breeze, and
Accumulate hot fermentation 5-10d, then dry or natural air drying or hot-air dry after it is standby；By the aspergillus niger described in claim 1
JXJA01 spore inoculating is in sterile PDA liquid medium or other aseptic liquid nutrient mediums, 28 DEG C, under conditions of 160rpm
After cultivating 2d, sprinkling is inoculated on sterile wheat bran and stalk mixed solid state cuture base, is cultivated 3-6d, is waited to grow a large amount of black spores
After son, according to certain ratio, it is well mixed with the dried solid mixture of accumulation hot fermentation processing, recycles comminutor pair
Mixture is granulated, that is, obtains the multi-functional aspergillus niger containing great amount of soluble potassium element, P elements and aspergillus niger work spore
Bacterial manure.
A kind of 8. preparation side of aspergillus niger JXJA01 solid multifunctionals bacterial manure and soil improvement renovation agent as claimed in claim 7
Method, it is characterised in that：Agricultural wastes, fowl and animal excrement, the mass ratio of ground phosphate rock and potassium breeze are 60:20:10:10.
A kind of 9. preparation side of aspergillus niger JXJA01 solid multifunctionals bacterial manure and soil improvement renovation agent as claimed in claim 7
Method, it is characterised in that：The mass ratio of wheat bran and stalk is 1 in wheat bran and stalk mixed solid state cuture base:1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710843897.8A CN107523508B (en) | 2017-09-15 | 2017-09-15 | Aspergillus niger JXJ A01 with activities of decomposing potassium and phosphorus and degrading organophosphorus pesticides and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710843897.8A CN107523508B (en) | 2017-09-15 | 2017-09-15 | Aspergillus niger JXJ A01 with activities of decomposing potassium and phosphorus and degrading organophosphorus pesticides and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107523508A true CN107523508A (en) | 2017-12-29 |
| CN107523508B CN107523508B (en) | 2020-09-08 |
Family
ID=60736030
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710843897.8A Active CN107523508B (en) | 2017-09-15 | 2017-09-15 | Aspergillus niger JXJ A01 with activities of decomposing potassium and phosphorus and degrading organophosphorus pesticides and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107523508B (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109022294A (en) * | 2018-08-29 | 2018-12-18 | 河南省科学院生物研究所有限责任公司 | Aspergillus niger J6 potassium decomposing culture medium and its application |
| CN109824445A (en) * | 2019-04-08 | 2019-05-31 | 杨荣勇 | The fruit tree health fertilizer of soil close friend a kind of and its application and preparation method |
| CN111718221A (en) * | 2020-05-14 | 2020-09-29 | 南京农业大学 | Composite soil remediation agent and preparation method thereof |
| CN113980822A (en) * | 2021-11-16 | 2022-01-28 | 青岛农业大学 | Saline-alkali-tolerant Aspergillus niger, application of Aspergillus niger as phosphate solubilizing fungus and application of Aspergillus niger in promotion of growth of saline-alkali soil corns |
| CN114231425A (en) * | 2022-01-13 | 2022-03-25 | 哈尔滨师范大学 | Phosphorus-dissolving potassium-solubilizing bacterium Aspergillus niger Z8 and application thereof |
| CN115505536A (en) * | 2022-10-12 | 2022-12-23 | 河北省科学院生物研究所 | Aspergillus niger CA-5 and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103436453A (en) * | 2013-09-03 | 2013-12-11 | 保罗生物园科技股份有限公司 | Aspergillus niger C2 and microbial inoculum thereof |
| CN105385638A (en) * | 2015-12-21 | 2016-03-09 | 云南省微生物发酵工程研究中心有限公司 | Microbial phosphorus-dissolving preparation and preparation method and application thereof |
-
2017
- 2017-09-15 CN CN201710843897.8A patent/CN107523508B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103436453A (en) * | 2013-09-03 | 2013-12-11 | 保罗生物园科技股份有限公司 | Aspergillus niger C2 and microbial inoculum thereof |
| CN105385638A (en) * | 2015-12-21 | 2016-03-09 | 云南省微生物发酵工程研究中心有限公司 | Microbial phosphorus-dissolving preparation and preparation method and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| PARIN C. SHAH ET AL: "Phytase production by Aspergillus niger NCIM 563 for a novel application to degrade organophosphorus pesticides", 《AMB EXPR》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109022294A (en) * | 2018-08-29 | 2018-12-18 | 河南省科学院生物研究所有限责任公司 | Aspergillus niger J6 potassium decomposing culture medium and its application |
| CN109824445A (en) * | 2019-04-08 | 2019-05-31 | 杨荣勇 | The fruit tree health fertilizer of soil close friend a kind of and its application and preparation method |
| CN111718221A (en) * | 2020-05-14 | 2020-09-29 | 南京农业大学 | Composite soil remediation agent and preparation method thereof |
| CN113980822A (en) * | 2021-11-16 | 2022-01-28 | 青岛农业大学 | Saline-alkali-tolerant Aspergillus niger, application of Aspergillus niger as phosphate solubilizing fungus and application of Aspergillus niger in promotion of growth of saline-alkali soil corns |
| CN114231425A (en) * | 2022-01-13 | 2022-03-25 | 哈尔滨师范大学 | Phosphorus-dissolving potassium-solubilizing bacterium Aspergillus niger Z8 and application thereof |
| CN115505536A (en) * | 2022-10-12 | 2022-12-23 | 河北省科学院生物研究所 | Aspergillus niger CA-5 and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107523508B (en) | 2020-09-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101659934B (en) | Antagonistic bacteria preventing and removing continuous cropping banana Panama wilt disease and microbial organic fertilizer thereof | |
| CN107523508A (en) | With potassium decomposing, the aspergillus niger JXJ A01 of phosphorus decomposing and degrading organic phosphor pesticides activity and its application | |
| CN103194405B (en) | Growth-promoting bacteria for promoting ginger growth and preventing and controlling continuous cropping ginger soil-borne wilt and microorganism organic fertilizer produced from growth-promoting bacteria | |
| CN101717315B (en) | Special peanut organic and inorganic compound fertilizer for biologically preventing and treating root knot nematode disease and preparation method thereof | |
| CN105316249B (en) | A kind of bacillus subtilis and microbial bacterial agent and their applications in fermentation maturity | |
| CN102442849A (en) | Disease-preventing bio-organic fertilizer prepared from straw and its preparation method | |
| CN101759493B (en) | Biological disease-preventing organic fertilizer and preparation method thereof | |
| CN112159761B (en) | Preparation method of penicillium oxalicum and application of penicillium oxalicum in phosphate solubilizing, growth promoting and fusarium graminearum antagonism | |
| CN101948780B (en) | Antagonist bacterium for preventing and treating continuous cropping hot pepper epidemic disease and microbial organic fertilizer thereof | |
| CN105199962A (en) | Microbial straw-decomposing agent as well as preparation method and application thereof | |
| CN112725213B (en) | Arthrobacter and application thereof as decomposing agent for decomposing vegetable straw | |
| CN101886055B (en) | Antagonistic bacteria NJL-14 for preventing and controlling continuous-cropping tobacco bacterial wilt | |
| CN101696395A (en) | Antagonistic bacterium for preventing and killing off continuous cropping tobacco black shank and microbial organic fertilizer thereof | |
| CN106007824B (en) | Composite bacterial fertilizer and preparation method and application thereof | |
| CN113321547A (en) | Rhizosphere growth promoting water-soluble microbial fertilizer and preparation method thereof | |
| CN103173387B (en) | Growth-promoting bacteria for facilitating growth of rape and microbial organic fertilizer | |
| CN110791459B (en) | Bacillus subtilis for preventing and controlling continuous cropping lily soil-borne blight and application thereof | |
| CN108383653A (en) | A kind of method and bio-organic fertilizer preparing fertilizer using stalk | |
| CN105624062B (en) | Eupatorium adenophorum organic fertilizer solid microbial inoculum, preparation method and application in organic fertilizer production | |
| CN107857681A (en) | A kind of special fertilizer for cole and preparation method and application | |
| CN111072420A (en) | Compound amino acid microbial agent and preparation method thereof | |
| CN109957535A (en) | Simple bacillus, microbial bacterial agent, bio-fertilizer and the application prepared using it | |
| CN115594549A (en) | Microbial organic fertilizer for improving saline-alkali soil plough layer structure and preparation method thereof | |
| WO2018215925A1 (en) | A biological soil conditioner | |
| Mohaseb et al. | Role of mineral and bio-fertilizers on some soil properties and rice productivity under reclaimed saline soils |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20200723 Address after: 332005 Jiujiang University, 551 Qianjin East Road, Jiangxi, Jiujiang Applicant after: JIUJIANG University Address before: 332000 Jiangxi province Jiujiang Xunyang Xunyang Road No. 320 District 2 Building 3 unit Applicant before: Zhang Binghuo |
|
| TA01 | Transfer of patent application right | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |