CN107793263A - The application of one plant of efficient phosphate-solubilizing bacterium - Google Patents
The application of one plant of efficient phosphate-solubilizing bacterium Download PDFInfo
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Abstract
The invention discloses one plant of efficient phosphate-solubilizing bacterium (deposit number CGMCC NO.12767) application, mainly in the application in preparing efficient phosphorous fertilizer and the application in microorganism composite seed coating agent is prepared, fermented first with the bacterial strain, obtain zymotic fluid, zymotic fluid is mixed with half-mature grain again, obtain fungal spore powder, finally by fungal spore powder and turf, precipitated calcium carbonate, phosphorus source is mixed to get efficient phosphorous fertilizer, or by fungal spore powder and film forming agent, insecticide, dispersant, protective agent, colouring agent and nutritional agents are mixed to get microorganism composite seed coating agent.The present invention is advantageous in that：(1) efficient phosphorous fertilizer being prepared, after can notable improved soil, the available phosphorus content utilized can be absorbed by crops by improving in soil, and then improve crop dry weight more than 8%；(2) the microorganism composite seed coating agent being prepared, after being wrapped in the surface of the seed, it is possible to increase crop absorbs the ability of available phosphorus, and then improves crop dry weight more than 7%.
Description
Technical field
The present invention relates to the application of one plant of efficient phosphate-solubilizing bacterium, belong to biological technical field.
Background technology
P elements are one of a great number of elements necessary to plant growth and development.Because most of P elements are anchored at soil
On earth granule so that can be very rare by P elements that plant directly absorbs in soil.
Although China is one of maximum country of whole world fertilizer amount, the P elements that can be utilized in soil by plant
Still it is deficient.Research shows that titanium pigment element content in many soil is 1ppm or less, largely constrains plant
The growth and development of thing.Excessively a variety of problem of environmental pollutions such as body eutrophication are caused using phosphate fertilizer.
Edaphon is Soil Nutrient Transformation and the power of circulation, and the profit of Contribution rate is improved using microorganism
It is effective method with rate.Phosphate solubilizing microorganism can secrete the organic acid of low molecule amount, such as oxalic acid, citric acid, H+Energy
The mineral P elements in soil are enough dissolved, the P elements anchored on soil granular are discharged, can in soil so as to improve
The content of Soluble phosphorus.
At present, the fungal bacterial strain such as many moulds, aspergillus, which has been reported, has the function that to dissolve set phosphorus in soil.Pass through
In soil inoculation can high-efficiency dissolution P elements microorganism, it is possible to increase available phosphorus content in soil, so as to increase wheat, big
The dry weight of the plants such as beans.There is research to apply Soluble phosphorus microorganism into soil (close to plant root), also there is research and utilization
P-dissolving fungi and the seed of rhizobium co-inoculation legume, to improve the intake of the phosphorus of plant.
However, it is micro- to improve the horizontal height degraded P elements living of available phosphorus in plant growing condition, increase soil system
Biological bacterial strain is still more rare, and has widespread demand.
The content of the invention
We have obtained a penicillium bacterial strain (deposit number CGMCC from corn soil by isolating and purifying
NO.12767), it has the phosphatic ability of efficient degradation, and the present invention surrounds the Penicillium strain, have studied it and is preparing height
Imitate the application of phosphate fertilizer, microorganism composite seed coating agent etc..
The present invention adopts the following technical scheme that：
First, application of the Penicillium strain (deposit number CGMCC NO.12767) in efficient phosphorous fertilizer is prepared
The method for preparing efficient phosphorous fertilizer is：
Step1：Fermented using the efficient phosphate-solubilizing bacterium that deposit number is CGMCC NO.12767, obtain zymotic fluid；
Step2：Make to obtain half-mature grain using wheat, corn quarrel and/or rice；
Step3：By above-mentioned zymotic fluid by the half-mature grain after sterilizing is inoculated into than 10%, then trained under the conditions of 25 DEG C
Grain is fully stirred after supporting 5 days, 5 days, then quiescent culture 10 days, co-culture 15 days；
Step4：After culture 15 days, by culture drying, crush, sieving, obtain fungal spore powder；
Step5：By the turf after above-mentioned fungal spore powder and high-temperature sterilization, precipitated calcium carbonate, phosphorus source with weight than 1000:
4000：10：100 ratio mixing, stirs, efficient phosphorous fertilizer is obtained after crushing.
Foregoing method, it is characterised in that in Step1, the process of fermentation is：
(1) Penicillium strain that deposit number is CGMCC NO.12767 is inoculated into MEA medium slants, at 28 DEG C
Culture obtains inclined-plane seed after 7 days；
(2) inclined-plane seed is inoculated into fluid nutrient medium, the composition of the fluid nutrient medium is：Sucrose 30g+ analysis for soybean powder
30g+FeSO4·7H200.005g+MnSO40.008g+H3BO30.002g+ZnSO40.005g+CuSO40.004g+
Na2MoO40.004g+CoCl20.002g+ inositol 0.04g, add distilled water to be assigned to 1L, initial pH value 5-6, cultivate at 29 DEG C, obtain
Obtain liquid seeds；
(3) liquid seeds are accessed in fermentation tank, the composition of fermentation medium is：Sucrose 30g+ analysis for soybean powder 30g+
FeSO4·7H200.005g+MnSO40.008g+H3BO30.002g+ZnSO40.005g+CuSO40.004g+Na2MoO40.004g+
CoCl20.002g+ inositol 0.04g, distilled water is added to be assigned to 1L, initial pH value 5-6, condition of culture：26-32 DEG C of temperature, stirring
Speed 150-250rpm, ferment 4-6 days, obtain zymotic fluid.
Foregoing method, it is characterised in that in Step2, the process for making half-mature grain is：
Wheat, corn quarrel and/or rice are soaked into a period of time in 55 DEG C of warm water, until grain moisture content is 30%,
Then cured 10 minutes at 100 DEG C, obtain half-mature grain.
2nd, application of the Penicillium strain (deposit number CGMCC NO.12767) in microorganism composite seed coating agent is prepared
Prepare microorganism composite seed coating agent method be：
Step1：Fermented using the efficient phosphate-solubilizing bacterium that deposit number is CGMCC NO.12767, obtain zymotic fluid；
Step2：Make to obtain half-mature grain using wheat, corn quarrel and/or rice；
Step3：By above-mentioned zymotic fluid by the half-mature grain after sterilizing is inoculated into than 10%, then trained under the conditions of 25 DEG C
Grain is fully stirred after supporting 5 days, 5 days, then quiescent culture 10 days, co-culture 15 days；
Step4：After culture 15 days, by culture drying, crush, sieving, obtain fungal spore powder；
Step5：Film forming agent is added in 50 DEG C of warm water, stirred to being completely dissolved, continues to add insecticide into warm water, divide
Powder, protective agent, colouring agent and nutritional agents, it is well mixed, suspending agent is made in the 4h that then milled in sand mill, most backward to suspend
Fungal spore powder is added in agent, mixes and produces microorganism composite seed coating agent.
Foregoing method, it is characterised in that in Step1, the process of fermentation is：
(1) Penicillium strain that deposit number is CGMCC NO.12767 is inoculated into MEA medium slants, at 28 DEG C
Culture obtains inclined-plane seed after 7 days；
(2) inclined-plane seed is inoculated into fluid nutrient medium, the composition of the fluid nutrient medium is：Sucrose 30g+ analysis for soybean powder
30g+FeSO4·7H200.005g+MnSO40.008g+H3BO30.002g+ZnSO40.005g+CuSO40.004g+
Na2MoO40.004g+CoCl20.002g+ inositol 0.04g, add distilled water to be assigned to 1L, initial pH value 5-6, cultivate at 29 DEG C, obtain
Obtain liquid seeds；
(3) liquid seeds are accessed in fermentation tank, the composition of fermentation medium is：Sucrose 30g+ analysis for soybean powder 30g+
FeSO4·7H200.005g+MnSO40.008g+H3BO30.002g+ZnSO40.005g+CuSO40.004g+Na2MoO40.004g+
CoCl20.002g+ inositol 0.04g, distilled water is added to be assigned to 1L, initial pH value 5-6, condition of culture：26-32 DEG C of temperature, stirring
Speed 150-250rpm, ferment 4-6 days, obtain zymotic fluid.
Foregoing method, it is characterised in that in Step2, the process for making half-mature grain is：
Wheat, corn quarrel and/or rice are soaked into a period of time in 55 DEG C of warm water, until grain moisture content is 30%,
Then cured 10 minutes at 100 DEG C, obtain half-mature grain.
Foregoing method, it is characterised in that in Step5, by percentage to the quality, make microorganism composite seed coating agent
The dosage of each raw material be respectively：
Foregoing method, it is characterised in that foregoing pesticidal agent is Metalaxyl-M, benefit reaches amine, thiram, iprodione, pyrrole worm
Quinoline, triticonazole, cypermethrin, Tebuconazole, desinsection be grand and Flutriafol in any one or any several mixing.
The present invention is advantageous in that：
(1) efficient phosphorous fertilizer being prepared, after can notable improved soil, improve soil in can be absorbed by crops profit
Available phosphorus content, and then improve crop dry weight more than 8%；
(2) the microorganism composite seed coating agent being prepared, after being wrapped in the surface of the seed, it is possible to increase crop, which absorbs, to be had
The ability of phosphorus is imitated, and then improves crop dry weight more than 7%.
Embodiment
Make specific introduce to the present invention below in conjunction with specific embodiment.
First, isolate and purify to obtain the Penicillium strain with efficient phosphate-solubilizing ability
We acquire different plant rhizosphere soils extensively in the whole country, isolated 125 plants of phosphate solubilizing microorganisms, warp
Dissolving phosphorus aptitude tests are crossed, the bacterial strain that numbering is MN10003 has most strong molten dissolving P capacity.
1st, process is isolated and purified
The process that isolates and purifies for the bacterial strain that numbering is MN10003 is：
The corn field soil for taking Gaomi City of Shandong Province is sample.5g pedotheques are weighed in 25ml centrifuge tubes, are added
0.1% sterile 15ml Tween 80 solution, after fully shaking mixes, by sample with 10-1、10-2、10-3、10-4、10-5Concentration ladder
Degree dilution.Draw 2ml 10-4The dilute solution of concentration, it is uniformly coated on being trained containing PDA (potato glucose) for a diameter of 9cm
Support in base flat board, add final concentration of 100ppm streptomysin and 100ppm chloramphenicol in culture medium in advance.25 DEG C of dark conditions
Lower culture 5 days, checks whether have new bacterium colony to occur in flat board daily.After there is new bacterium colony, bacterium colony is purified, chosen in colony edge
Mycelia is taken, is seeded in time in new PDA plate, forms the pure bacterium colony of fungi.
2nd, phosphorus aptitude tests are dissolved
(1) the Soluble phosphorus efficiency on different phosphate sources culture medium
With Penicillium bilaiaeATCC20851 and bacillus megaterium Bacillus
MegateriumATCC14581 is control strain, and the bacterial strain for being MN10003 using numbering is test strain, and we are to three plants of bacterial strains
Soluble phosphorus circle (mm) and colony growth diameter (mm) on different phosphate sources Soluble phosphorus culture medium compare, as a result as follows：
As a result show：The bacterial strain that numbering is MN10003 has degradation capability for organic phosphorus sources and inorganic phosphorous sources.
(2) difficultly-soluble phosphates are the phosphate solubilization for the bacterial strain that numbering is MN10003 in the fluid nutrient medium of unique phosphorus source
Be separately added into the fluid nutrient medium using difficultly-soluble phosphates as unique phosphorus source numbering be MN10003 bacterial strain and
Penicillium bilaiaeATCC20851 bacterial strains, 27 DEG C of culture 10d, do not connect bacterium using blank as control (CK).
Liquid Culture based formulas is：(NH4)2SO40.5g/L, NaCl 0.2g/L, KCl 0.2g/L, MgSO4 7H2O
0.03g/L, MnSO40.03g/L, FeSO40.003g/L, dusty yeast 0.5g/L, glucose 10.0g/L, ground phosphate rock (cross 100 mesh
Sieve) 5.0g/L.
In fluid nutrient medium, the phosphorus decomposing speed of different phosphorus decomposing fungies is as follows：
As a result show：
(1) MN10003 and ATCC20851 has an obvious effect of solubilizing phosphate, significant difference between bacterial strain；
(2) when nitrogen source is ammonium nitrogen, MN10003 cultivates by 10d, and the water-soluble phosphorus in 50ml culture mediums is
10.3mg, account for and apply the 41.2% of ground phosphate rock total amount, be significantly higher than ATCC20851；
(3) when nitrogen source is nitrate nitrogen, MN10003 cultivates by 10d, and the water-soluble phosphorus in 50ml culture mediums is
13.9mg, account for the 55.6% of ground phosphate rock total amount.
3rd, tolerance is tested
With Penicillium bilaiaeATCC20851 and bacillus megaterium Bacillus
MegateriumATCC14581 is control strain, and for the bacterial strain for being MN10003 using numbering as test strain, we test three plants
Bacterial strain is for the half lethal concentration LC50 of different chemical agents, as a result following (unit：mg/ml)：
As a result show：The bacterial strain that numbering is MN10003 has stronger tolerance to existing chemical pesticide.
In summary, compared with having reported bacterial strain, the bacterial strain that numbering is MN10003 has stronger dissolving soil P elements
Ability, while tolerance is stronger, can cooperate with use with a variety of chemical pesticides, have a extensive future.
4th, to the influence of corn growth
Microbial inoculum has been made using conventional method by the bacterial strain for being MN10003 is numbered in we, in different soil environments, training
After supporting 30d, the biomass of corn in basin is tested, the following (unit of test result：G/ basins)：
In table, No. 1 soil is derived from Shanxi Province Yaodu District farmland, is light loamy soil, and available phosphorus is 17.9 μ g/g；No. 2 soil
Earth is derived from Shanxi Province Quwo County mill ridge farmland, is middle loamy texture moisture soil, the μ g/g of available phosphorus 10.3；No. 3 soil are derived from Shanxi Province's song
Fertile county mill ridge, to shelve wasteland, the μ g/g of available phosphorus 5.1 for many years.
As a result show：Growth of the bacterial strain that numbering is MN10003 to corn has facilitation.
5th, preservation
On July 12nd, 2016, we have been deposited in China General Microbiological culture presevation by the bacterial strain for being MN10003 is numbered
Center, preservation address are Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, and deposit number is CGMCC NO.12767, classification life
Name：Mould (Penicillium sp.).
2nd, fermented using above-mentioned Penicillium strain
1st, test tube slant seed culture
By Penicillium strain (deposit number is CGMCC NO.12767), being inoculated into MEA culture mediums, (malt extract 30g+ is big
Legumin peptone 3g+ agar 15g, distilled water is added to be assigned to 1L, sterilize 15min after wiring solution-forming at 121 DEG C, and pH is adjusted to 5.6 ± 0.2)
On inclined-plane.Inclined-plane seed is obtained after being cultivated 7 days at 28 DEG C.
2nd, strain liquid seed culture
Fluid nutrient medium：Sucrose 30g+ analysis for soybean powder 30g+FeSO4·7H200.005g+MnSO40.008g+H3BO30.002g+
ZnSO40.005g+CuSO40.004g+Na2MoO40.0 04g+CoCl20.002g+ inositol 0.04g, add distilled water to be assigned to 1L, just
Beginning pH value is 5-6.
Cultivated at 29 DEG C to suitable concn.Suitably increase air flux in incubation, be advantageous to bacteria liquid fermentation
Produce substantial amounts of spore.
3rd, strain fermentation tank mass propgation
Fermentation medium：Sucrose 30g+ analysis for soybean powder 30g+FeSO4·7H200.005g+MnSO40.008g+H3BO30.002g+
ZnSO40.005g+CuSO40.004g+Na2MoO40.004g+CoCl20.002g+ inositol 0.04g, add distilled water to be assigned to 1L, initially
PH value is 5-6.
Liquid seeds are accessed in (20-1000L) fermentation tank in 1-5% (V/V) ratio, condition of culture：Temperature 26-32
DEG C, mixing speed 150-250rpm, ferment 4-6 days, obtain zymotic fluid.
3rd, fungal spore powder is prepared using above-mentioned zymotic fluid
1st, half-mature grain is prepared
Wheat, corn quarrel and/or rice are soaked into a period of time (40 minutes or so) in 55 DEG C of warm water, until cereal contains
Water is 30%, is then cured 10 minutes at 100 DEG C, obtains half-mature grain.
Half-mature grain is sterilized 90 minutes under 121 DEG C, 101kPa, it is stand-by.
2nd, it is inoculated with, cultivates
By above-mentioned zymotic fluid by the half-mature grain after sterilizing is inoculated into than 10%, then cultivated 5 days under the conditions of 25 DEG C,
Fully stir grain after 5 days, then quiescent culture 10 days, co-culture 15 days.
3rd, fungal spore powder is obtained
After culture 15 days, by culture drying, crush, sieving, obtain fungal spore powder.
After testing, the concentration of effective viable bacteria is 4 × 10 in conidia powder8Individual/g.
4th, efficient phosphorous fertilizer is prepared using above-mentioned fungal spore powder
By the turf after above-mentioned fungal spore powder and high-temperature sterilization, precipitated calcium carbonate, phosphorus source with weight than 1000:4000:
10:100 ratio mixing, stirs, efficient phosphorous fertilizer is obtained after crushing.Wherein, phosphorus source both can be organic phosphorus sources (such as：
Lecithin) or inorganic phosphorous sources (such as：Ca8H2PO4)。
The light loamy soil from Shanxi Province Yaodu District farmland is chosen, available phosphorus content is 17.9 μ g/g, using the present invention
Efficient phosphorous fertilizer 60d after, the dry weight of crop is tested, so calculate crop dry weight growth rate.Result of calculation is as follows：
As can be seen here, efficient phosphorous fertilizer of the invention can significantly improve the dry weight of crop.
5th, microorganism composite seed coating agent is prepared using above-mentioned fungal spore powder
1st, active component
(Metalaxyl-M, benefit reach amine, thiram, iprodione, imidacloprid, triticonazole, chlorine cyanogen chrysanthemum for fungal spore powder, insecticide
Ester, Tebuconazole, desinsection be grand and Flutriafol in any one or any several mixing).
2nd, auxiliary material
Protective agent (glycerine), colouring agent (sunset yellow), nutritional agents (sodium sulphate), film forming agent (xanthans, carboxymethyl cellulose
Any one in sodium, chitosan and starch or any several mixing), dispersant (agriculture breast 602 and/or 2-3%NNO).
3rd, it is formulated (by percentage to the quality)
| Fungal spore powder | 14-26% |
| Insecticide | 1-5% |
| Protective agent | 1-4% |
| Colouring agent | 1-3% |
| Nutritional agents | 2-6% |
| Film forming agent | 20-60% |
| Dispersant | 2-3% |
| Water | Supply 100% |
4th, preparation method
By film forming agent add 50 DEG C of warm water in, stir to being completely dissolved, continue into warm water add insecticide, dispersant,
Protective agent, colouring agent and nutritional agents, it is well mixed, suspending agent is made in the 4h that then milled in sand mill, is added into suspending agent
Fungal spore powder, mix and produce microorganism composite seed coating agent.
5th, using effect
1st group：Fungal spore powder 14%, insecticide 1%, protective agent 1%, colouring agent 1%, nutritional agents 2%, film forming agent
20%th, dispersant 2%, water 59%.
2nd group：Fungal spore powder 20%, insecticide 3%, protective agent 2%, colouring agent 2%, nutritional agents 4%, film forming agent
30%th, dispersant 2%, water 37%.
3rd group：Fungal spore powder 26%, insecticide 5%, protective agent 4%, colouring agent 3%, nutritional agents 6%, film forming agent
40%th, dispersant 3%, water 13%.
After seed coat agent 60d using the present invention, the dry weight of crop is tested, and then calculates the growth of crop dry weight
Rate.Result of calculation is as follows：
As can be seen here, seed coat agent of the invention can significantly improve the dry weight of crop.
It should be noted that the invention is not limited in any way for above-described embodiment, it is all to use equivalent substitution or equivalent change
The technical scheme that the mode changed is obtained, all falls within protection scope of the present invention.
Claims (10)
1. one plant of efficient phosphate-solubilizing bacterium, deposit number is CGMCC NO.12767, its application in efficient phosphorous fertilizer is prepared.
2. application according to claim 1, it is characterised in that the method for preparing efficient phosphorous fertilizer is：
Step1：Fermented using the efficient phosphate-solubilizing bacterium that deposit number is CGMCC NO.12767, obtain zymotic fluid；
Step2：Make to obtain half-mature grain using wheat, corn quarrel and/or rice；
Step3：By above-mentioned zymotic fluid by the half-mature grain after sterilizing is inoculated into than 10%, then 5 are cultivated under the conditions of 25 DEG C
My god, fully stir grain after 5 days, then quiescent culture 10 days, co-culture 15 days；
Step4：After culture 15 days, by culture drying, crush, sieving, obtain fungal spore powder；
Step5：By the turf after above-mentioned fungal spore powder and high-temperature sterilization, precipitated calcium carbonate, phosphorus source with weight than 1000:
4000：10：100 ratio mixing, stirs, efficient phosphorous fertilizer is obtained after crushing.
3. application according to claim 2, it is characterised in that in Step1, the process of fermentation is：
(1) Penicillium strain that deposit number is CGMCC NO.12767 is inoculated into MEA medium slants, cultivated at 28 DEG C
Inclined-plane seed is obtained after 7 days；
(2) inclined-plane seed is inoculated into fluid nutrient medium, the composition of the fluid nutrient medium is：Sucrose 30g+ analysis for soybean powder 30g+
FeSO4·7H200.005g+MnSO40.008g+H3BO30.002g+ZnSO40.005g+CuSO40.004g+Na2MoO40.004g+
CoCl20.002g+ inositol 0.04g, add distilled water to be assigned to 1L, initial pH value 5-6, cultivate at 29 DEG C, obtain liquid seeds；
(3) liquid seeds are accessed in fermentation tank, the composition of fermentation medium is：Sucrose 30g+ analysis for soybean powder 30g+FeSO4·
7H200.005g+MnSO40.008g+H3BO30.002g+ZnSO40.005g+CuSO40.004g+Na2MoO40.004g+
CoCl20.002g+ inositol 0.04g, distilled water is added to be assigned to 1L, initial pH value 5-6, condition of culture：26-32 DEG C of temperature, stirring
Speed 150-250rpm, ferment 4-6 days, obtain zymotic fluid.
4. application according to claim 2, it is characterised in that in Step2, the process for making half-mature grain is：
Wheat, corn quarrel and/or rice are soaked into a period of time in 55 DEG C of warm water, until grain moisture content is 30%, then
Cured 10 minutes at 100 DEG C, obtain half-mature grain.
5. one plant of efficient phosphate-solubilizing bacterium, deposit number is CGMCC NO.12767, and it is in microorganism composite seed coating agent is prepared
Using.
6. application according to claim 5, it is characterised in that prepare microorganism composite seed coating agent method be：
Step1：Fermented using the efficient phosphate-solubilizing bacterium that deposit number is CGMCC NO.12767, obtain zymotic fluid；
Step2：Make to obtain half-mature grain using wheat, corn quarrel and/or rice；
Step3：By above-mentioned zymotic fluid by the half-mature grain after sterilizing is inoculated into than 10%, then 5 are cultivated under the conditions of 25 DEG C
My god, fully stir grain after 5 days, then quiescent culture 10 days, co-culture 15 days；
Step4：After culture 15 days, by culture drying, crush, sieving, obtain fungal spore powder；
Step5：Film forming agent is added in 50 DEG C of warm water, stirred to being completely dissolved, continues to add insecticide into warm water, disperse
Agent, protective agent, colouring agent and nutritional agents, it is well mixed, suspending agent is made in the 4h that then milled in sand mill, most backward suspending agent
Middle addition fungal spore powder, mixes and produces microorganism composite seed coating agent.
7. application according to claim 6, it is characterised in that in Step1, the process of fermentation is：
(1) Penicillium strain that deposit number is CGMCC NO.12767 is inoculated into MEA medium slants, cultivated at 28 DEG C
Inclined-plane seed is obtained after 7 days；
(2) inclined-plane seed is inoculated into fluid nutrient medium, the composition of the fluid nutrient medium is：Sucrose 30g+ analysis for soybean powder 30g+
FeSO4·7H200.005g+MnSO40.008g+H3BO30.002g+ZnSO40.005g+CuSO40.004g+Na2MoO40.004g+
CoCl20.002g+ inositol 0.04g, add distilled water to be assigned to 1L, initial pH value 5-6, cultivate at 29 DEG C, obtain liquid seeds；
(3) liquid seeds are accessed in fermentation tank, the composition of fermentation medium is：Sucrose 30g+ analysis for soybean powder 30g+FeSO4·
7H200.005g+MnSO40.008g+H3BO30.002g+ZnSO40.005g+CuSO40.004g+Na2MoO40.004g+
CoCl20.002g+ inositol 0.04g, distilled water is added to be assigned to 1L, initial pH value 5-6, condition of culture：26-32 DEG C of temperature, stirring
Speed 150-250rpm, ferment 4-6 days, obtain zymotic fluid.
8. application according to claim 6, it is characterised in that in Step2, the process for making half-mature grain is：
Wheat, corn quarrel and/or rice are soaked into a period of time in 55 DEG C of warm water, until grain moisture content is 30%, then
Cured 10 minutes at 100 DEG C, obtain half-mature grain.
9. application according to claim 6, it is characterised in that in Step5, by percentage to the quality, make microorganism
The dosage of each raw material of composite seed coating agent is respectively：
10. application according to claim 9, it is characterised in that the insecticide be Metalaxyl-M, benefit up to amine, thiram,
Iprodione, imidacloprid, triticonazole, cypermethrin, Tebuconazole, desinsection be grand and Flutriafol in any one or it is any several
Mixing.
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Cited By (2)
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| CN114368987A (en) * | 2022-02-26 | 2022-04-19 | 山东三方化工集团有限公司 | Soil activation type microbial fertilizer and preparation method thereof |
| CN117136968A (en) * | 2022-09-15 | 2023-12-01 | 慕恩（北京）生物科技有限公司 | Composite microbial composition and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101444227A (en) * | 2009-01-09 | 2009-06-03 | 北京理工大学 | Multifunctional biological seed coating agent and preparation method thereof |
| US9365464B2 (en) * | 2012-11-16 | 2016-06-14 | Novozymes Bioag A/S | Microbial strains, compositions, and methods for increasing available phosphate for plants |
| CN103695318B (en) * | 2013-12-20 | 2015-11-11 | 云南金雨庄生物科技有限责任公司 | A kind of phosphorus decomposing fungi and utilize its fermentation edible fungi residue to prepare the method for biologic phosphorus fertilizer |
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| CN114368987A (en) * | 2022-02-26 | 2022-04-19 | 山东三方化工集团有限公司 | Soil activation type microbial fertilizer and preparation method thereof |
| CN117136968A (en) * | 2022-09-15 | 2023-12-01 | 慕恩（北京）生物科技有限公司 | Composite microbial composition and application thereof |
| CN117136968B (en) * | 2022-09-15 | 2024-05-24 | 慕恩（北京）生物科技有限公司 | Composite microbial composition and application thereof |
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