CN106336329A - Microbial bacterial fertilizer special for honeysuckles and preparation method thereof - Google Patents
Microbial bacterial fertilizer special for honeysuckles and preparation method thereof Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C05—FERTILISERS; MANUFACTURE THEREOF
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- C12N1/20—Bacteria; Culture media therefor
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Abstract
The invention discloses a microbial bacterial fertilizer special for honeysuckles and a preparation method thereof. The raw materials for the microbial bacterial fertilizer include cereus bacillus powder, bacillus amyloliquefaciens powder, streptomyces fimicarius powder, streptomyces bellus powder, trichoderma saturniforme powder, potassium fulvic acid, amino acid powder and medium trace elements. In the microbial bacterial fertilizer, the used five strains can be directly separated from soil, have a rhizosphere growth-promoting effect, have phosphate-solubilizing, nitrogen-fixation and potassium-releasing effects, can directionally secrete certain secondary metabolites to rhizospheres while breeding and growing on the crops surfaces and in plants or soil and can promote nutrient absorption of plants and stimulate plant growth to play the effects of the fertilizer. The microbial bacterial fertilizer is safe to human and livestock and is environmentally friendly, and the preparation method is simple, low in cost and simple to use.
Description
Technical field
The invention belongs to microbial manure technical field is and in particular to a kind of Flos Lonicerae microbial bacterial manure special and its preparation
Method.
Background technology
Flos Lonicerae is Caprifoliaceae, belongs to perennial evergreen voluble shrub, as one of China's parts of generic medicinal plants, with the flower being dried
Flower bud or the flower just opened and stem and leaf are used as medicine.Flos Lonicerae implantation methods include at present, and one is wild planting, and uncontrolled, harvest is few.Two
For the Conventional wisdom fertilization method carrying out by rule of thumb, this method is random big, dose greatly then utilization rate of fertilizer low, production cost is high,
Also to ecological environment, and few volume increase advantage that can not give full play to forest of applying fertilizer.And adopt empiric fertilization method
Also easily make soil acidification serious, soil compaction, Soil degradation, utilization rate of fertilizer reduces, or even seedling death phenomenon.And using existing
Some fertilizing method gained dry flowers yield poorly, and institute's pan Flos Lonicerae poor quality, and its Content of Chlorogenic Acid is 2.5%, and resistance and adaptability
Difference, have impact on income and the enthusiasm for production of flower-grower.
Mention a kind of compound method of Chinese herbal medicament nutrient fertilizer containing honeysuckle in cn200810022062, the method by dried poultrymanure,
Dry sheep manure, dry cow dung, dry rabbit excrement, soybean cake powder, bone meal, corn cob, beanstalk, compound fertilizer, carbamide, phosphate fertilizer, glucose powder, carbendazim
Powder, dichlorvos powder and phenolic resin mixing after fermentation are obtained the fertilizer being exclusively used in Flos Lonicerae.In feces of livestock and poultry and plant albuminoid
Effective ingredient is different, and extraction fermentation process is also different, and the method, only by various conventional fertilizer materials, mixes after fermentation.Not
Processed for different fertilizer materials, thus fertilizer efficiency undesirable it is impossible to accomplish to learn from other's strong points to offset one's weaknesses.For raising Flos Lonicerae yield
Effect is limited.
Wu Jianping discloses a kind of Fertilizer special for honeysuckle flower and preparation method thereof in patent No. 201110338055x, its
In this Fertilizer special for honeysuckle flower, comprising: the N P and K of 18-28 weight portion amino acid fertilizer, 17-27 weight portion fertilizer and requirement
Fertile.The Fertilizer special for honeysuckle flower that the present invention provides provides the Chlorogenic Acid of Flos Lonicerae of fertilizer, luteoloside than not applying the present invention
Content exceeds 12. 9% and 69. 64 respectively, and improvement quality effect is substantially, inconspicuous to effect of increasing production.
Disclose a kind of Special active fertilizer for honeysuckle and preparation method thereof to iron army etc. in the patent No. 201210990604.
Choose protease producing strains and add rapeseed cake 50-70%, solid after sesame seed meal 20-40%, bean cake 10-20% mixing by weight percentage
Crude protein in fermentation enzymolysis grouts forms it into the biofermentation cake fertilizer with biological activity, then with activating agent and inorganic fertilizer
Material (each raw materials by weight example is: activating agent 8-10%, biofermentation cake fertilizer 20-30%, inorganic fertilizer 60-80%) is uniformly
After mixture, pelletize obtains final product Special active fertilizer for honeysuckle material.Before administration Special active fertilizer for honeysuckle of the present invention can not only meet Flos Lonicerae
The available nutrient demand of phase growth, reaches early stage and strengthens the purpose that bud sends out branch, and organic fertilizer therein can carry for Later growth
For continuous nutrient, long-lasting maintenance fertilizer efficiency, promote tree body robust growth, to carrying of the yield and quality in Flos Lonicerae later stage
The significant effect of Gao Junyou, but its consumption is big, and cost of material is high, and peasant household's acceptance level is low；Fertilizer from existing disclosed Flos Lonicerae
Mainly inorganic fertilizer from the point of view of material, fertilizer two big class, the also not related art scheme about the special microbial-bacterial fertilizer of Flos Lonicerae
Open.
Content of the invention
Problem to be solved by this invention is to develop a kind of Flos Lonicerae microbial bacterial manure special, prepares this microbial-bacterial fertilizer
Raw material be by weight: season human relations series bacillus mycopowder 10-30 part, bacillus amyloliquefaciens mycopowder 20-40 part, excrement give birth to strepto-
Bacterium mycopowder 20-40 part, beautiful streptomycete mycopowder 10-30 part, Saturn spore Trichoderma spp. mycopowder 20-40 part, potassium fulvate 20-60 part, ammonia
The former powder 10-30 part of base acid；Middle trace element 0.1-0.2 part；The strain feature that this bacterial manure compounds is mainly strong adaptability, stability
Good, energy symbiotic co-existence, rationally utilize the interaction between microorganism, the fermentation technique that produces being equipped with high-tech produces high-quality
Product.This 5 plants of bacterial strains used in the present invention are all can be directly isolated to obtain from soil, all have root mark growth-promoting functions,
There is phosphorus decomposing, fixed nitrogen, potassium decomposing acts on, can be in Radix Flos Lonicerae, stem in leaf surface or soil while flourish, and orients gold
Flos Lonicerae rhizosphere secretes some secondary metabolites, it is possible to increase Flos Lonicerae to the absorption of nutrient, stimulate its growth to play fertilizer
Effect；Microbial-bacterial fertilizer of the present invention, to people, animal safety, belongs to environmentally friendly, and preparation method of the present invention is simple, use cost
Low, use is simple.
A kind of another purpose, there is provided preparation method of Flos Lonicerae microbial bacterial manure special, its concrete preparation method, bag
Include following steps:
Step one, the preparation of season human relations series bacillus mycopowder
Take out season human relations series bacillus preservation pipe, draw flat board respectively with nitrogen-free solid medium and recovered, 30 DEG C of cultures 72 are little
When.Under flat board, picking single bacterium colony is seeded to equipped with nitrogen-free solid medium, cultivates 72 hours in 30 DEG C of incubators, uses
3000ml sterilized water is seeded to the lawn eluting in three Fructus Solani melongenae bottles equipped with 300l season human relations series bacillus seed culture medium
In 500l fermentation tank, open stirring 120r/min, ventilation is 100l/min within first 8 hours, after 8-24 hour, ventilation is 200l/
Min, after 24 hours, ventilation is 300l/min, 30 DEG C of culture 32-48 hours, treats that total bacteria count content is not less than 2,000,000,000 cfu/
Ml, you can as season human relations series bacillus seed liquor；
Fermentation: the season human relations series bacillus seed liquor of above-mentioned preparation is seeded to season human relations series bacillus with the ratio of 10%-20%
On solid medium, mix, in tray top fermentation, windrow thickness is 5-10cm, controls fermentation temperature to be 30-40 DEG C, and ferment 36-
48 hours, treat that spore content is not less than 5,000,000,000 cfu/g, you can dry, treat that moisture is less than 10%, pulverized 100 mesh sieves, that is,
Obtain season human relations series bacillus mycopowder；
Wherein, described nitrogen-free solid medium: sucrose 10g, dipotassium hydrogen phosphate 0.2g, magnesium sulfate 0.2g, sodium chloride 0.2g, sulphuric acid
Calcium 0.1g, Calcium Carbonate 5g, distilled water 1000ml, agar 18g, ph7.2；
Wherein, described season human relations series bacillus seed culture medium: molasses 20g/l, corn starch 10 g/l, peanut meal powder 30 g/
L, magnesium sulfate 0.4 g/l, manganese sulfate 0.6g/l, potassium dihydrogen phosphate 0.6 g/l, Calcium Carbonate 10 g/l, ph7.0；
Season human relations series bacillus solid medium: distiller's grains of beer 57%, wheat bran 35%, cottonseed meal 5%, stone powder 1%, thick skin of Semen Maydis 2%, carbamide
0.4%, dipotassium hydrogen phosphate 0.4%, magnesium sulfate 0.05%, moisture content in medium 50%-60%.The condition of each medium sterilization is:
0.10-0.15mpa, 121 DEG C sterilize 30 minutes.
Step 2, the preparation of bacillus amyloliquefaciens mycopowder
Take out bacillus amyloliquefaciens preservation pipe, draw flat board respectively with nutrient solid medium and recovered, 30 DEG C of cultures
48 hours.Under flat board, picking single bacterium colony is seeded to equipped with nutrient solid medium, cultivates 48 in 30 DEG C of incubators
Hour, with 3000ml sterilized water by the lawn eluting in three Fructus Solani melongenae bottles, it is seeded to equipped with 300l bacillus amyloliquefaciens liquid
In the 500l fermentation tank of seed culture medium, open stirring 120r/min, ventilation is 200l/min within first 12 hours, ventilates after 12 hours
Measure as 320l/min, 30 DEG C of culture 16-24 hours, treat that total bacterial content is not less than 1,000,000,000 cfu/ml, you can as solution starch spore
Bacillus liquid seeds；
Fermentation: the bacillus amyloliquefaciens liquid seeds of above-mentioned preparation are added to bacillus amyloliquefaciens solid fermentation culture medium
In, mix, in tray top fermentation, windrow thickness is 5-10cm, controls fermentation temperature to be 30-40 DEG C, 36-48 hour of fermenting is treated
Spore content is not less than 5,000,000,000 cfu/g, you can dry, and treats that moisture is less than 10%, pulverizes 40 mesh sieves, that is, obtain solving starch
Bacillus cereuss mycopowder；
Wherein, described nutrient solid medium: peptone 5g, Carnis Bovis seu Bubali cream 3g, sodium chloride 5g, agar 20 g, water 1000ml,
ph7.2；
Wherein, described bacillus amyloliquefaciens liquid seed culture medium: glucose 8g/l, Semen Maydis powder 10 g/l, Semen Glycines powder 10 g/
L, magnesium sulfate 0.2 g/l, manganese sulfate 0.1 g/l, potassium dihydrogen phosphate 0.5 g/l, disodium hydrogen phosphate 1.0 g/l, ph7.0；
Wherein, bacillus amyloliquefaciens solid fermentation culture medium: wheat bran 60%, skin of Semen Maydis 35%, cottonseed meal 2%, stone powder 3%, carbamide
0.4%, dipotassium hydrogen phosphate 0.4%, magnesium sulfate 0.05%, manganese sulfate 0.01%, moisture content in medium 50%-60%.Each medium sterilization
Condition be: 0.10-0.15mpa, 121 DEG C sterilize 30 minutes.
Step 3, the preparation of streptomyces fimicarius mycopowder
Take out streptomyces fimicarius culture presevation pipe, draw flat board with Gause I solid medium and recovered, cultivate 7 days for 30 DEG C.
Under flat board, the streak inoculation of picking single bacterium colony is equipped with the Fructus Solani melongenae bottle of 150 milliliters of Gause I solid mediums, in incubator
In 30 DEG C cultivate 6-8 days, treat that lawn covers with Fructus Solani melongenae bottle, produce a large amount of spore powders and can use 500 milliliters of physiological saline solution to wash
De-, regulation spore concentration is 0.1 hundred million cfu/ml, as streptomyces fimicarius seed liquor；
Fermentation: the streptomyces fimicarius solid that the streptomyces fimicarius seed liquor of above-mentioned preparation is seeded to sterilizing with 3% inoculum concentration is sent out
In ferment culture medium, after strain is mixed homogeneously with streptomyces fimicarius solid medium, the streptomyces fimicarius solid fermentation planted will be connected
Culture medium is spread out on fermentation tank, and height of materials is 3-6cm, 28-32 DEG C of temperature control, and air humidity remains 60%-75%, mistake within first day
Air humidity remains 40%-50% afterwards, ferments 3-4 days, detects that its spore content is not less than 2,000,000,000 cfu/g, you can low-temperature air-drying
It is less than 8% to moisture, pulverized 40 mesh sieves, that is, obtain streptomyces fimicarius mycopowder；
Wherein, described Gause I solid medium: potassium nitrate: 1 gram, soluble starch: 20 grams, dipotassium hydrogen phosphate: 05
Gram, magnesium sulfate: 05 grams, sodium chloride: 05 grams, ferrous sulfate: 0 01 grams, agar: 20 grams, distilled water supplies 1000ml, ph7
2～7 4；
Wherein, described streptomyces fimicarius solid fermentation culture medium: bagasse 80%, bean cake 2%, maize cob meal 1%, stone powder 10%,
Wheat bran 7%, manganese sulfate 0.004%, dipotassium hydrogen phosphate 0.1%, magnesium sulfate 0.05%, solid medium water content is 40%-60%.Each training
The condition of foster base sterilizing is: 0.10-0.15mpa, 121 DEG C sterilize 30 minutes.
Step 4, the preparation of beautiful streptomycete mycopowder
Take out beautiful streptomyces species preservation pipe, draw flat board with Gause I solid medium and recovered, cultivate 7 days for 30 DEG C.
Under flat board, the streak inoculation of picking single bacterium colony is equipped with the Fructus Solani melongenae bottle of 150 milliliters of Gause I solid mediums, in incubator
In 30 DEG C cultivate 6-8 days, treat that lawn covers with Fructus Solani melongenae bottle, produce a large amount of spore powders and can use 500 milliliters of physiological saline solution to wash
De-, regulation spore concentration is 0.1 hundred million cfu/ml, as beautiful streptomycete seed liquor；
Fermentation: the beautiful streptomycete solid that the beautiful streptomycete seed liquor of above-mentioned preparation is seeded to sterilizing with 5% inoculum concentration is sent out
In ferment culture medium, after strain is mixed homogeneously with beautiful streptomycete solid medium, the beautiful strepto- bacteria solid fermentation planted will be connected
Culture medium is spread out on fermentation tank, and height of materials is 5-8cm, 28-32 DEG C of temperature control, and a few days ago air humidity remains 60%-75%, mistake
Air humidity remains 40%-50% afterwards, ferments 4-6 days, detects that its spore content is not less than 1,000,000,000 cfu/g, you can low-temperature air-drying
Moisture is less than 8%, pulverized 40 mesh sieves, that is, obtains beautiful streptomycete mycopowder；
Wherein, described Gause I solid medium: potassium nitrate: 1 gram, soluble starch: 20 grams, dipotassium hydrogen phosphate: 05
Gram, magnesium sulfate: 05 grams, sodium chloride: 05 grams, ferrous sulfate: 0 01 grams, agar: 20 grams, distilled water supplies 1000ml, ph7
2～7 4；
Wherein, described beautiful strepto- bacteria solid fermentation culture medium: Testa oryzae 80%, cottonseed meal 2%, Semen Maydis powder 1%, stone powder 2%, wheat bran
15%, manganese sulfate 0.004%, dipotassium hydrogen phosphate 0.1%, magnesium sulfate 0.05%, solid medium water content is 40%-60%.Each culture
The condition of base sterilizing is: 0.10-0.15mpa, and 121 DEG C sterilize 30 minutes.
Step 5, the preparation of Saturn spore Trichoderma spp. mycopowder
The preparation of Saturn spore Trichoderma spp. seed liquor: take out Saturn spore Trichoderma spp. strain preservation pipe, draw flat board with pda solid medium and carry out
Recovery, cultivates 6 days for 30 DEG C.Under flat board, the streak inoculation of picking single bacterium colony is equipped with the Fructus Solani melongenae bottle of 150 milliliters of pda solid mediums
In, cultivate 4-6 days for 30 DEG C in incubator, treat that lawn covers with Fructus Solani melongenae bottle, produce the aseptic life that a large amount of spores can use 500 milliliters
Reason salt water, regulation spore concentration is 0.1 hundred million cfu/ml, as Saturn spore Trichoderma spp. seed liquor；
Fermentation: Saturn spore Trichoderma spp. solid that Saturn spore Trichoderma spp. seed liquor of above-mentioned preparation is seeded to sterilizing with 2% inoculum concentration is sent out
In ferment culture medium, after strain is mixed homogeneously with Saturn spore Trichoderma spp. solid medium, Saturn spore Trichoderma spp. solid fermentation planted will be connected
Culture medium is spread out on fermentation tank, and height of materials is 5-10cm, 28-32 DEG C of temperature control, and humidity remains 60%-75%, ferments 4-6 days,
Detect that its total spore content is not less than 3,000,000,000 cfu/g, you can low-temperature air-drying, treat that moisture is less than 10%, pulverized 100 mesh sieves,
Obtain Saturn spore Trichoderma spp. mycopowder；
Wherein, described pda solid medium: Rhizoma Solani tuber osi 200g, sucrose 20g, water 1000ml, agar 20g；
Wherein, described Saturn spore Trichoderma spp. solid fermentation culture medium: bagasse 85%, the sesame residues dregs of rice 2%, corn cob 5%, stone powder 4%,
Thick skin of Semen Maydis 2%, carbamide 0.4%, dipotassium hydrogen phosphate 0.4%, magnesium sulfate 0.05%, moisture content in medium 50%-60%；Each culture medium is gone out
The condition of bacterium is: 0.10-0.15mpa, and 121 DEG C sterilize 30 minutes.
Step 6, by the various mycopowder made above quarterly human relations series bacillus mycopowder 10-30 part, bacillus amyloliquefaciens
Mycopowder 20-40 part, streptomyces fimicarius mycopowder 20-40 part, beautiful streptomycete mycopowder 10-30 part, Saturn spore Trichoderma spp. mycopowder 20-40
Part, potassium fulvate 20-60 part, aminoacid former powder 10-30 part；Middle trace element 0.1-0.2 part mixing, uniformly, as Flos Lonicerae
Microbial bacterial manure special.
Wherein, the using method of described Flos Lonicerae microbial bacterial manure special is: fertilising trench digging ring is applied first, every mu of administration
Measure as 2-5 kg；Second fertilization time is squaring period, and every mu of amount of application is 1-2 kg, dilutes 100 times of -200 times of foliage-sprays；
Apply foliage-spray once within every 20-30 days below, every mu of amount of application is 1-2 kg, dilutes 100 times of -200 times of foliage-sprays；Every year
Foliage-spray 2-4 time.
Wherein, described middle trace element is by 14 parts of edta disodium, 5 parts of ferrous sulfate, 10 parts of zinc sulfate, manganese chloride 0.3
Part, 3 parts of boric acid, 0.02 part of cobaltous chloride, 0.01 part of copper chloride, 0.002 part of Nickel dichloride., 3 parts of compositions of sodium molybdate.
N in soil, p element are the indispensable elements of plant growing, but substantial amounts of p in soil with insoluble metal chelate
Existing. the streptomyces fimicarius in the present invention and beautiful streptomycete, bacillus amyloliquefaciens can produce phosphatase, in solubilized soil
P element, advantageously promotes plant absorption, thus the administration reducing phosphate fertilizer also can reach the effect of phosphorus supplement；In addition, Saturn spore is wooden
Mould, in the mycorhiza (am) that streptomyces fimicarius can also induce in Flos Lonicerae with beautiful streptomycete, the spore germination of funguses, is conducive to bacterium
Root one funguses one plant mutualism, promotes the growth of Flos Lonicerae.
Contain season human relations series bacillus and bacillus amyloliquefaciens in the present invention, this two bacillus be all from soil and
Separate in plant and obtain, heat-resisting, resistance spore can be produced, there is significant Biocontrol Potential；Season human relations series bacillus and solution
Bacillus amyloliquefacienses as root mark growth-promoting microorganism, they can parasitic roots, leaf, and other plant surface.Plant can be planted a colony rapidly
Root hair, leaveves, and other surfaces, can prevent the foundation of pathomycete and antibacterial.In addition, season human relations series bacillus have stronger
The ability of fixed nitrogen, can preferably play the effect of fixed nitrogen in soil.
Beneficial effects of the present invention and advantage: by by the microbial-bacterial fertilizer ditch spread of the present invention to Radix Flos Lonicerae mark, improving
The microenvironment of Radix Flos Lonicerae mark periphery, so that loosing soil is breathed freely, plays retain water and nutrients effect, simultaneously works as phosphorus decomposing, fixed nitrogen,
Potassium decomposing acts on, beneficial to the growth of Radix Flos Lonicerae mark, simultaneously in the Lonicera japonica phase, foliage-spray microbial-bacterial fertilizer of the present invention, its
In microorganism quickly in the stem of Flos Lonicerae, leaf, take field planting, promote with Flos Lonicerae pass through Foliage Absorption Macro and microelements,
Aminoacid etc., greatly promotes its yield and quality, and through field experiment, rate of growth has reached 44.66%, applies the present invention simultaneously
The average content of the Chlorogenic Acid of Flos Lonicerae of Flos Lonicerae microbial bacterial manure special and flavonoid increases by 16.57% He respectively
18.83%, play the effect of obvious increasing yield and improving quality.
The strain feature that this bacterial manure compounds is mainly strong adaptability, good stability, energy symbiotic co-existence, rationally utilizes microorganism
Between interaction, the fermentation technique that produces being equipped with high-tech produces the product of high-quality.This 5 plants of bacterium used in the present invention
Strain is all can be directly isolated to obtain from soil, all has root mark growth-promoting functions, has phosphorus decomposing, fixed nitrogen, potassium decomposing acts on, can be in gold
In Flos Lonicerae root, stem, leaf surface or soil while flourish, and orient the Flos Lonicerae rhizosphere some secondary metabolites of secretion,
Can improve Flos Lonicerae to the absorption of nutrient, stimulate its growth to play the effect of fertilizer；Microbial-bacterial fertilizer of the present invention is pacified to people, animal
Entirely, belong to environmentally friendly, preparation method of the present invention is simple, use cost is low, use is simple.
Embodiment 1
A kind of Flos Lonicerae microbial bacterial manure special, the raw material preparing this microbial-bacterial fertilizer is by weight: season human relations series bacillus
Mycopowder 10-30 part, bacillus amyloliquefaciens mycopowder 20-40 part, streptomyces fimicarius mycopowder 20-40 part, beautiful streptomycete mycopowder 10-
30 parts, Saturn spore Trichoderma spp. mycopowder 20-40 part, potassium fulvate 20-60 part, aminoacid former powder 10-30 part；Middle trace element 0.1-
0.2 part；Its concrete preparation method, comprises the following steps:
Step one, the preparation of season human relations series bacillus mycopowder
(paenibacillus jilunlii, purchased from Chinese agriculture microorganism fungus kind to take out accc03231 season human relations series bacillus
Preservation administrative center) preservation pipe, draw flat board respectively with nitrogen-free solid medium and recovered, cultivate 72 hours for 30 DEG C.In flat board
Lower picking single bacterium colony is seeded to equipped with nitrogen-free solid medium, cultivates 72 hours in 30 DEG C of incubators, aseptic with 3000ml
Water, by the lawn eluting in three Fructus Solani melongenae bottles, is seeded to the 500l fermentation tank equipped with 300l season human relations series bacillus seed culture medium
In, open stirring 120r/min, ventilation is 100l/min within first 8 hours, after 8-24 hour, ventilation is 200l/min, 24 hours
Ventilation is 300l/min afterwards, 30 DEG C of culture 32-48 hours, treats that total bacteria count content is not less than 2,000,000,000 cfu/ml, you can as season
Human relations series bacillus seed liquor；
Fermentation: the season human relations series bacillus seed liquor of above-mentioned preparation is seeded to season human relations series bacillus with the ratio of 10%-20%
On solid medium, mix, in tray top fermentation, windrow thickness is 5-10cm, controls fermentation temperature to be 30-40 DEG C, and ferment 36-
48 hours, treat that spore content is not less than 5,000,000,000 cfu/g, you can dry, treat that moisture is less than 10%, pulverized 100 mesh sieves, that is,
Obtain season human relations series bacillus mycopowder；
Wherein, described nitrogen-free solid medium: sucrose 10g, dipotassium hydrogen phosphate 0.2g, magnesium sulfate 0.2g, sodium chloride 0.2g, sulphuric acid
Calcium 0.1g, Calcium Carbonate 5g, 18g, distilled water 1000ml, ph7.2；
Wherein, described season human relations series bacillus seed culture medium: molasses 20g/l, corn starch 10 g/l, peanut meal powder 30 g/
L, magnesium sulfate 0.4 g/l, manganese sulfate 0.6g/l, potassium dihydrogen phosphate 0.6 g/l, Calcium Carbonate 10 g/l, ph7.0.Each culture medium is gone out
The condition of bacterium is: 0.10-0.15mpa, and 121 DEG C sterilize 30 minutes；
Season human relations series bacillus solid medium: distiller's grains of beer 57%, wheat bran 35%, cottonseed meal 5%, stone powder 1%, thick skin of Semen Maydis 2%, carbamide
0.4%, dipotassium hydrogen phosphate 0.4%, magnesium sulfate 0.05%, moisture content in medium 50%-60%.The condition of each medium sterilization is:
0.10-0.15mpa, 121 DEG C sterilize 30 minutes.
Step 2, the preparation of bacillus amyloliquefaciens mycopowder
(bacillus amyloliquefaciens, this strain is by this to take out cgmcc no.11856 bacillus amyloliquefaciens
Patentee in China Committee for Culture Collection of Microorganisms's common micro-organisms center's preservation it has been disclosed that) preservation pipe, use nutrition
Gravy solid medium is drawn flat board respectively and is recovered, and cultivates 48 hours for 30 DEG C.Under flat board, picking single bacterium colony is seeded to dress
Nutritious gravy solid medium, cultivates 48 hours, with 3000ml sterilized water by three Fructus Solani melongenae bottles in 30 DEG C of incubators
Lawn eluting, is seeded in the 500l fermentation tank equipped with 300l bacillus amyloliquefaciens liquid seed culture medium, opens stirring 120r/
Min, ventilation is 200l/min within first 12 hours, and after 12 hours, ventilation is 320l/min, 30 DEG C of culture 16-24 hours, treats total
Bacterial content is not less than 1,000,000,000 cfu/ml, you can as bacillus amyloliquefaciens liquid seeds；
Fermentation: the bacillus amyloliquefaciens liquid seeds of above-mentioned preparation are added to bacillus amyloliquefaciens solid fermentation culture medium
In, mix, in tray top fermentation, windrow thickness is 5-10cm, controls fermentation temperature to be 30-40 DEG C, 36-48 hour of fermenting is treated
Spore content is not less than 5,000,000,000 cfu/g, you can dry, and treats that moisture is less than 10%, pulverizes 40 mesh sieves, that is, obtain solving starch
Bacillus cereuss mycopowder；
Wherein, described nutrient solid medium: peptone 5g, Carnis Bovis seu Bubali cream 3g, sodium chloride 5g, agar 20 g, water 1000ml,
ph7.2；
Wherein, described bacillus amyloliquefaciens liquid seed culture medium: glucose 8g/l, Semen Maydis powder 10 g/l, Semen Glycines powder 10 g/
L, magnesium sulfate 0.2 g/l, manganese sulfate 0.1 g/l, potassium dihydrogen phosphate 0.5 g/l, disodium hydrogen phosphate 1.0 g/l, ph7.0；
Wherein, bacillus amyloliquefaciens solid fermentation culture medium: wheat bran 60%, skin of Semen Maydis 35%, cottonseed meal 2%, stone powder 3%, carbamide
0.4%, dipotassium hydrogen phosphate 0.4%, magnesium sulfate 0.05%, manganese sulfate 0.01%, moisture content in medium 50%-60%.Each medium sterilization
Condition be: 0.10-0.15mpa, 121 DEG C sterilize 30 minutes.
Step 3, the preparation of streptomyces fimicarius mycopowder
(streptomyces fimicarius protects purchased from Chinese agriculture microorganism fungus kind to take out accc40451 streptomyces fimicarius
Hide administrative center) culture presevation pipe, draw flat board with Gause I solid medium and recovered, cultivate 7 days for 30 DEG C.Under flat board
Picking single bacterium colony streak inoculation, equipped with the Fructus Solani melongenae bottle of 150 milliliters of Gause I solid mediums, is trained for 30 DEG C in incubator
Foster 6-8 days, treat that lawn covers with Fructus Solani melongenae bottle, produce the physiological saline solution eluting that a large amount of spore powders can use 500 milliliters, adjust spore
Sub- concentration is 0.1 hundred million cfu/ml, as streptomyces fimicarius seed liquor；
Fermentation: the streptomyces fimicarius solid that the streptomyces fimicarius seed liquor of above-mentioned preparation is seeded to sterilizing with 3% inoculum concentration is sent out
In ferment culture medium, after strain is mixed homogeneously with streptomyces fimicarius solid medium, the streptomyces fimicarius solid fermentation planted will be connected
Culture medium is spread out on fermentation tank, and height of materials is 3-6cm, 28-32 DEG C of temperature control, and air humidity remains 60%-75%, mistake within first day
Air humidity remains 40%-50% afterwards, ferments 3-4 days, detects that its spore content is not less than 2,000,000,000 cfu/g, you can low-temperature air-drying
It is less than 8% to moisture, pulverized 40 mesh sieves, that is, obtain streptomyces fimicarius mycopowder；
Wherein, described Gause I solid medium: potassium nitrate: 1 gram, soluble starch: 20 grams, dipotassium hydrogen phosphate: 05
Gram, magnesium sulfate: 05 grams, sodium chloride: 05 grams, ferrous sulfate: 0 01 grams, agar: 20 grams, distilled water supplies 1000ml, ph7
2～7 4；
Wherein, described streptomyces fimicarius solid fermentation culture medium: bagasse 80%, bean cake 2%, maize cob meal 1%, stone powder 10%,
Wheat bran 7%, manganese sulfate 0.004%, dipotassium hydrogen phosphate 0.1%, magnesium sulfate 0.05%, solid medium water content is 40%-60%.Each training
The condition of foster base sterilizing is: 0.10-0.15mpa, 121 DEG C sterilize 30 minutes.
Step 4, the preparation of beautiful streptomycete mycopowder
(streptomyces bellus, purchased from Chinese agriculture Microbiological Culture Collection pipe to take out accc40649 beauty streptomycete
Reason center) culture presevation pipe, draw flat board with Gause I solid medium and recovered, cultivate 7 days for 30 DEG C.Picking under flat board
Single bacterium colony streak inoculation, equipped with the Fructus Solani melongenae bottle of 150 milliliters of Gause I solid mediums, cultivates 6-8 for 30 DEG C in incubator
My god, treat that lawn covers with Fructus Solani melongenae bottle, produce the physiological saline solution eluting that a large amount of spore powders can use 500 milliliters, adjust spore dense
Spend for 0.1 hundred million cfu/ml, as beautiful streptomycete seed liquor；
Fermentation: the beautiful streptomycete solid that the beautiful streptomycete seed liquor of above-mentioned preparation is seeded to sterilizing with 5% inoculum concentration is sent out
In ferment culture medium, after strain is mixed homogeneously with beautiful streptomycete solid medium, the beautiful strepto- bacteria solid fermentation planted will be connected
Culture medium is spread out on fermentation tank, and height of materials is 5-8cm, 28-32 DEG C of temperature control, and a few days ago air humidity remains 60%-75%, mistake
Air humidity remains 40%-50% afterwards, ferments 4-6 days, detects that its spore content is not less than 1,000,000,000 cfu/g, you can low-temperature air-drying
Moisture is less than 8%, pulverized 40 mesh sieves, that is, obtains beautiful streptomycete mycopowder；
Wherein, described Gause I solid medium: potassium nitrate: 1 gram, soluble starch: 20 grams, dipotassium hydrogen phosphate: 05
Gram, magnesium sulfate: 05 grams, sodium chloride: 05 grams, ferrous sulfate: 0 01 grams, agar: 20 grams, distilled water supplies 1000ml, ph7
2～7 4；
Wherein, described beautiful strepto- bacteria solid fermentation culture medium: Testa oryzae 80%, cottonseed meal 2%, Semen Maydis powder 1%, stone powder 2%, wheat bran
15%, manganese sulfate 0.004%, dipotassium hydrogen phosphate 0.1%, magnesium sulfate 0.05%, solid medium water content is 40%-60%.Each culture
The condition of base sterilizing is: 0.10-0.15mpa, and 121 DEG C sterilize 30 minutes.
Step 5, the preparation of Saturn spore Trichoderma spp. mycopowder
The preparation of Saturn spore Trichoderma spp. seed liquor:
(trichoderma saturnisporum, purchased from Chinese agriculture microorganism fungus kind to take out accc32233 Saturn spore Trichoderma spp.
Preservation administrative center) culture presevation pipe, draw flat board with pda solid medium and recovered, cultivate 6 days for 30 DEG C.Choose under flat board
Take single bacterium colony streak inoculation equipped with the Fructus Solani melongenae bottle of 150 milliliters of pda solid mediums, cultivate 4-6 days for 30 DEG C in incubator,
Treat that lawn covers with Fructus Solani melongenae bottle, produce the physiological saline solution eluting that a large amount of spores can use 500 milliliters, adjusting spore concentration is
0.1 hundred million cfu/ml, as Saturn spore Trichoderma spp. seed liquor；
Fermentation: Saturn spore Trichoderma spp. solid that Saturn spore Trichoderma spp. seed liquor of above-mentioned preparation is seeded to sterilizing with 2% inoculum concentration is sent out
In ferment culture medium, after strain is mixed homogeneously with Saturn spore Trichoderma spp. solid medium, Saturn spore Trichoderma spp. solid fermentation planted will be connected
Culture medium is spread out on fermentation tank, and height of materials is 5-10cm, 28-32 DEG C of temperature control, and humidity remains 60%-75%, ferments 4-6 days,
Detect that its total spore content is not less than 3,000,000,000 cfu/g, you can low-temperature air-drying, treat that moisture is less than 10%, pulverized 100 mesh sieves,
Obtain Saturn spore Trichoderma spp. mycopowder；
Wherein, described pda solid medium: Rhizoma Solani tuber osi 200g, sucrose 20g, water 1000ml, agar 20g；
Wherein, described Saturn spore Trichoderma spp. solid fermentation culture medium: bagasse 85%, the sesame residues dregs of rice 2%, corn cob 5%, stone powder 4%,
Thick skin of Semen Maydis 2%, carbamide 0.4%, dipotassium hydrogen phosphate 0.4%, magnesium sulfate 0.05%, moisture content in medium 50%-60%；Each culture medium is gone out
The condition of bacterium is: 0.10-0.15mpa, and 121 DEG C sterilize 30 minutes.
Step 6, by the various mycopowder made above quarterly human relations series bacillus mycopowder 10-30 part, bacillus amyloliquefaciens
Mycopowder 20-40 part, streptomyces fimicarius mycopowder 20-40 part, beautiful streptomycete mycopowder 10-30 part, Saturn spore Trichoderma spp. mycopowder 20-40
Part, potassium fulvate 20-60 part, aminoacid former powder 10-30 part；Middle trace element 0.1-0.2 part mixing, uniformly, as Flos Lonicerae
Microbial bacterial manure special.
Wherein, described middle trace element is by 14 parts of edta disodium, 5 parts of ferrous sulfate, 10 parts of zinc sulfate, manganese chloride 0.3
Part, 3 parts of boric acid, 0.02 part of cobaltous chloride, 0.01 part of copper chloride, 0.002 part of Nickel dichloride., 3 parts of compositions of sodium molybdate.
Embodiment 2
The following detailed description of the present invention Flos Lonicerae microbial bacterial manure special Flos Lonicerae crop field test service condition.
1st, material and method
1) experimental field overview
Test week certain Planting household prosperous in Hunan Province Shaoyang City Longhui County.Flos Lonicerae material to be tested is the general cultivation product of life in local 3 years
Kind；
2) EXPERIMENTAL DESIGN
Using field plot contrast test.Test sets 2 process: (1) Flos Lonicerae of the present invention microbial bacterial manure special, first
Secondary every mu of amount of application of fertilising is 3 kg, applies 15g for equivalent every plant, fertilization time is on April 1st, 2013, applies fertilizer with matched group simultaneously；
Second fertilization time is on May 2nd, 2013, and every mu of amount of application is 1 kg, dilutes 100 times of foliage-sprays；When third time is applied fertilizer
Between be on June 1st, 2013, every mu of amount of application is 1kg, dilutes 100 times of foliage-sprays；.(2) compare, for local common Chemical Mixed Fertilizer,
Every mu of amount of application is 100 k g, applies 0.5kg for equivalent every plant.If 3 groups parallel, every group of parallel plot area is 667m2, fertilising
Mode is that distance flower Dun25-30cmChu trench digging ring is applied, and test fertilizer is imposed in ditch, earthing by ditch depth 15cm.Other management all with
Field production is identical, only controls one unitary variant of fertilizer.
3) mensure of yield index
Alabastrum measures: carries out in the 6-7 month in 2013, surveyed index: alabastrum length, hundred flower buds weight (dry flower).
4) mensure of quality index
The detection method of chlorogenic acid and total flavonoid is carried out according to 2010 editions detection methods of Flos Lonicerae hplc pharmacopeia.
2nd, result and analysis
1) impact to Lonicera japonica growth and yield for the fertilising
Flos Lonicerae plant can only breed alabastrum on newborn branch, and not regrowth alabastrum in old branch, the sending out of therefore newborn branch
Situation of educating will directly affect the yield of Flos Lonicerae, and it is good and bad that synkaingenesis branch number can also embody plant growing way.Of the present invention
Flos Lonicerae microbial bacterial manure special contain multiple-microorganism bacterium, they can be directly isolated to obtain from soil, has phosphorus decomposing, Gu
Nitrogen, potassium decomposing, all there are root mark growth-promoting functions, in crop surface, plant inside or soil while flourish, and can orient
Plant rhizosphere secretes some secondary metabolites, it is possible to increase plant plays fertilizer to the absorption of nutrient, stimulation plant strain growth
Effect, also contains the middle and trace element such as boron, magnesium, zinc and humic acidss, has multiple biological activities, can preferably meet Flos Lonicerae
The needs of vine growth and development.Bud after applying the Flos Lonicerae microbial bacterial manure special of the present invention, on Flos Lonicerae new life branch
Differentiation capability is remarkably reinforced, and blossoms more；Alabastrum is long as described in Table 1, and alabastrum substance is all substantially variant than matched group, gold
The yield of Flos Lonicerae is significantly improved, and rate of growth has reached 44.66%.
The impact to Lonicera japonica growth and yield for the table 1 Flos Lonicerae microbial bacterial manure special
| Process | Difference flower joint number | Alabastrum length (cm) | Alabastrum weight (g) | Produce yield (kg/ mu) | Rate of growth (%) |
| Microbial-bacterial fertilizer | 7.32 | 5.45 | 1.9724 | 119.2 | 44.66 |
| Blank | 6.24 | 4.96 | 1.7103 | 82.4 |
2) impact to quality of Flos Lonicerae for the Flos Lonicerae microbial bacterial manure special
The principle active component of Flos Lonicerae is chlorogenic acid and Flavonoid substances, the height direct reaction of chlorogenic acid and Flavone content
Go out the height of the economic worth of Flos Lonicerae, therefore weigh the quality of Flos Lonicerae with the content of chlorogenic acid and flavonoid.Fertilising is right
The impact of Chlorogenic Acid of Flos Lonicerae and Flavone content is shown in Table 2.
The impact to quality of Flos Lonicerae for the table 2 Flos Lonicerae microbial bacterial manure special
| Process | Chlorogenic acid (%) | Increase rate (%) | Flavonoid | Produce increase rate (%) |
| Microbial-bacterial fertilizer | 3.152 | 16.57 | 11.04 | 18.83 |
| Blank | 2.704 | 9.29 |
Table 2 shows, after applying Flos Lonicerae microbial bacterial manure special of the present invention, Chlorogenic Acid of Flos Lonicerae and flavonoid
Content can occur different degrees of change, applies Chlorogenic Acid of Flos Lonicerae and the Huang of Flos Lonicerae microbial bacterial manure special of the present invention
The average content of ketone increases by 16.57% and 18.83% respectively.As can be seen here, apply the special micro- life of Flos Lonicerae of the present invention
Thing bacterial manure can significantly improve the content of Chlorogenic Acid of Flos Lonicerae and flavonoid, directly improve Flos Lonicerae medicinal quality and
Economic worth.
Claims (3)
1. a kind of Flos Lonicerae microbial bacterial manure special it is characterized in that, the raw material preparing this microbial-bacterial fertilizer is by weight: season
Human relations series bacillus mycopowder 10-30 part, bacillus amyloliquefaciens mycopowder 20-40 part, streptomyces fimicarius mycopowder 20-40 part, beautiful
Streptomycete mycopowder 10-30 part, Saturn spore Trichoderma spp. mycopowder 20-40 part, potassium fulvate 20-60 part, aminoacid former powder 10-30 part；In
Trace element 0.1-0.2 part；Its concrete preparation method, comprises the following steps:
Step one, the preparation of season human relations series bacillus mycopowder
Take out season human relations series bacillus preservation pipe, draw flat board respectively with nitrogen-free solid medium and recovered, 30 DEG C of cultures 72 are little
When, under flat board, picking single bacterium colony is seeded to equipped with nitrogen-free solid medium, cultivates 72 hours in 30 DEG C of incubators, uses
3000ml sterilized water is seeded to the lawn eluting in three Fructus Solani melongenae bottles equipped with 300l season human relations series bacillus seed culture medium
In 500l fermentation tank, open stirring 120r/min, ventilation is 100l/min within first 8 hours, after 8-24 hour, ventilation is 200l/
Min, after 24 hours, ventilation is 300l/min, 30 DEG C of culture 32-48 hours, treats that total bacteria count content is not less than 2,000,000,000 cfu/
Ml, you can as season human relations series bacillus seed liquor；
Fermentation: the season human relations series bacillus seed liquor of above-mentioned preparation is seeded to season human relations series bacillus with the ratio of 10%-20%
On solid medium, mix, in tray top fermentation, windrow thickness is 5-10cm, controls fermentation temperature to be 30-40 DEG C, and ferment 36-
48 hours, treat that spore content is not less than 5,000,000,000 cfu/g, you can dry, treat that moisture is less than 10%, pulverized 100 mesh sieves, that is,
Obtain season human relations series bacillus mycopowder；
Wherein, described nitrogen-free solid medium: sucrose 10g, dipotassium hydrogen phosphate 0.2g, magnesium sulfate 0.2g, sodium chloride 0.2g, sulphuric acid
Calcium 0.1g, Calcium Carbonate 5g, agar 18g, distilled water 1000ml, ph7.2；
Wherein, described season human relations series bacillus seed culture medium: molasses 20g/l, corn starch 10 g/l, peanut meal powder 30 g/
L, magnesium sulfate 0.4 g/l, manganese sulfate 0.6g/l, potassium dihydrogen phosphate 0.6 g/l, Calcium Carbonate 10 g/l, ph7.0；
Season human relations series bacillus solid medium: distiller's grains of beer 57%, wheat bran 35%, cottonseed meal 5%, stone powder 1%, thick skin of Semen Maydis 2%, carbamide
0.4%, dipotassium hydrogen phosphate 0.4%, magnesium sulfate 0.05%, moisture content in medium 50%-60%；The condition of each medium sterilization is:
0.10-0.15mpa, 121 DEG C sterilize 30 minutes；
Step 2, the preparation of bacillus amyloliquefaciens mycopowder
Take out bacillus amyloliquefaciens preservation pipe, draw flat board respectively with nutrient solid medium and recovered, 30 DEG C of cultures
48 hours, under flat board, picking single bacterium colony was seeded to equipped with nutrient solid medium, cultivated 48 in 30 DEG C of incubators
Hour, with 3000ml sterilized water by the lawn eluting in three Fructus Solani melongenae bottles, it is seeded to equipped with 300l bacillus amyloliquefaciens liquid
In the 500l fermentation tank of seed culture medium, open stirring 120r/min, ventilation is 200l/min within first 12 hours, ventilates after 12 hours
Measure as 320l/min, 30 DEG C of culture 16-24 hours, treat that total bacterial content is not less than 1,000,000,000 cfu/ml, you can as solution starch spore
Bacillus liquid seeds；
Fermentation: the bacillus amyloliquefaciens liquid seeds of above-mentioned preparation are added to bacillus amyloliquefaciens solid fermentation culture medium
In, mix, in tray top fermentation, windrow thickness is 5-10cm, controls fermentation temperature to be 30-40 DEG C, 36-48 hour of fermenting is treated
Spore content is not less than 5,000,000,000 cfu/g, you can dry, and treats that moisture is less than 10%, pulverizes 40 mesh sieves, that is, obtain solving starch
Bacillus cereuss mycopowder；
Wherein, described nutrient solid medium: peptone 5g, Carnis Bovis seu Bubali cream 3g, sodium chloride 5g, agar 20 g, water 1000ml,
ph7.2；
Wherein, described bacillus amyloliquefaciens liquid seed culture medium: glucose 8g/l, Semen Maydis powder 10 g/l, Semen Glycines powder 10 g/
L, magnesium sulfate 0.2 g/l, manganese sulfate 0.1 g/l, potassium dihydrogen phosphate 0.5 g/l, disodium hydrogen phosphate 1.0 g/l, ph7.0；
Wherein, bacillus amyloliquefaciens solid fermentation culture medium: wheat bran 60%, skin of Semen Maydis 35%, cottonseed meal 2%, stone powder 3%, carbamide
0.4%, dipotassium hydrogen phosphate 0.4%, magnesium sulfate 0.05%, manganese sulfate 0.01%, moisture content in medium 50%-60%；Each medium sterilization
Condition be: 0.10-0.15mpa, 121 DEG C sterilize 30 minutes；
Step 3, the preparation of streptomyces fimicarius mycopowder
Take out streptomyces fimicarius culture presevation pipe, draw flat board with Gause I solid medium and recovered, cultivate 7 days for 30 DEG C,
Under flat board, the streak inoculation of picking single bacterium colony is equipped with the Fructus Solani melongenae bottle of 150 milliliters of Gause I solid mediums, in incubator
In 30 DEG C cultivate 6-8 days, treat that lawn covers with Fructus Solani melongenae bottle, produce a large amount of spore powders and can use 500 milliliters of physiological saline solution to wash
De-, regulation spore concentration is 0.1 hundred million cfu/ml, as streptomyces fimicarius seed liquor；
Fermentation: the streptomyces fimicarius solid that the streptomyces fimicarius seed liquor of above-mentioned preparation is seeded to sterilizing with 3% inoculum concentration is sent out
In ferment culture medium, after strain is mixed homogeneously with streptomyces fimicarius solid medium, the streptomyces fimicarius solid fermentation planted will be connected
Culture medium is spread out on fermentation tank, and height of materials is 3-6cm, 28-32 DEG C of temperature control, and air humidity remains 60%-75%, mistake within first day
Air humidity remains 40%-50% afterwards, ferments 3-4 days, detects that its spore content is not less than 2,000,000,000 cfu/g, you can low-temperature air-drying
It is less than 8% to moisture, pulverized 40 mesh sieves, that is, obtain streptomyces fimicarius mycopowder；
Wherein, described Gause I solid medium: potassium nitrate: 1 gram, soluble starch: 20 grams, dipotassium hydrogen phosphate: 0.5
Gram, magnesium sulfate: 0.5 gram, sodium chloride: 0.5 gram, ferrous sulfate: 0.01 gram, agar: 20 grams, 1000ml supplied by distilled water,
Ph7.2～7.4；
Wherein, described streptomyces fimicarius solid fermentation culture medium: bagasse 80%, bean cake 2%, maize cob meal 1%, stone powder 10%,
Wheat bran 7%, manganese sulfate 0.004%, dipotassium hydrogen phosphate 0.1%, magnesium sulfate 0.05%, solid medium water content is 40%-60%；Each training
The condition of foster base sterilizing is: 0.10-0.15mpa, 121 DEG C sterilize 30 minutes；
Step 4, the preparation of beautiful streptomycete mycopowder
Take out beautiful streptomyces species preservation pipe, draw flat board with Gause I solid medium and recovered, cultivate 7 days for 30 DEG C,
Under flat board, the streak inoculation of picking single bacterium colony is equipped with the Fructus Solani melongenae bottle of 150 milliliters of Gause I solid mediums, in incubator
In 30 DEG C cultivate 6-8 days, treat that lawn covers with Fructus Solani melongenae bottle, produce a large amount of spore powders and can use 500 milliliters of physiological saline solution to wash
De-, regulation spore concentration is 0.1 hundred million cfu/ml, as beautiful streptomycete seed liquor；
Fermentation: the beautiful streptomycete solid that the beautiful streptomycete seed liquor of above-mentioned preparation is seeded to sterilizing with 5% inoculum concentration is sent out
In ferment culture medium, after strain is mixed homogeneously with beautiful streptomycete solid medium, the beautiful strepto- bacteria solid fermentation planted will be connected
Culture medium is spread out on fermentation tank, and height of materials is 5-8cm, 28-32 DEG C of temperature control, and a few days ago air humidity remains 60%-75%, mistake
Air humidity remains 40%-50% afterwards, ferments 4-6 days, detects that its spore content is not less than 1,000,000,000 cfu/g, you can low-temperature air-drying
Moisture is less than 8%, pulverized 40 mesh sieves, that is, obtains beautiful streptomycete mycopowder；
Wherein, described Gause I solid medium: potassium nitrate: 1 gram, soluble starch: 20 grams, dipotassium hydrogen phosphate: 0.5
Gram, magnesium sulfate: 0.5 gram, sodium chloride: 0.5 gram, ferrous sulfate: 0.01 gram, agar: 20 grams, 1000ml supplied by distilled water,
Ph7.2～7.4；
Wherein, described beautiful strepto- bacteria solid fermentation culture medium: Testa oryzae 80%, cottonseed meal 2%, Semen Maydis powder 1%, stone powder 2%, wheat bran
15%, manganese sulfate 0.004%, dipotassium hydrogen phosphate 0.1%, magnesium sulfate 0.05%, solid medium water content is 40%-60%；Each culture
The condition of base sterilizing is: 0.10-0.15mpa, and 121 DEG C sterilize 30 minutes；
Step 5, the preparation of Saturn spore Trichoderma spp. mycopowder
The preparation of Saturn spore Trichoderma spp. seed liquor: take out Saturn spore Trichoderma spp. strain preservation pipe, draw flat board with pda solid medium and carry out
Recovery, cultivates 6 days for 30 DEG C, under flat board, the streak inoculation of picking single bacterium colony is equipped with the Fructus Solani melongenae bottle of 150 milliliters of pda solid mediums
In, cultivate 4-6 days for 30 DEG C in incubator, treat that lawn covers with Fructus Solani melongenae bottle, produce the aseptic life that a large amount of spores can use 500 milliliters
Reason salt water, regulation spore concentration is 0.1 hundred million cfu/ml, as Saturn spore Trichoderma spp. seed liquor；
Fermentation: Saturn spore Trichoderma spp. solid that Saturn spore Trichoderma spp. seed liquor of above-mentioned preparation is seeded to sterilizing with 2% inoculum concentration is sent out
In ferment culture medium, after strain is mixed homogeneously with Saturn spore Trichoderma spp. solid medium, Saturn spore Trichoderma spp. solid fermentation planted will be connected
Culture medium is spread out on fermentation tank, and height of materials is 5-10cm, 28-32 DEG C of temperature control, and humidity remains 60%-75%, ferments 4-6 days,
Detect that its total spore content is not less than 3,000,000,000 cfu/g, you can low-temperature air-drying, treat that moisture is less than 10%, pulverized 100 mesh sieves,
Obtain Saturn spore Trichoderma spp. mycopowder；
Wherein, described pda solid medium: Rhizoma Solani tuber osi 200g, sucrose 20g, water 1000ml, agar 20g；
Wherein, described Saturn spore Trichoderma spp. solid fermentation culture medium: bagasse 85%, the sesame residues dregs of rice 2%, corn cob 5%, stone powder 4%,
Thick skin of Semen Maydis 2%, carbamide 0.4%, dipotassium hydrogen phosphate 0.4%, magnesium sulfate 0.05%, moisture content in medium 50%-60%；Each culture medium is gone out
The condition of bacterium is: 0.10-0.15mpa, and 121 DEG C sterilize 30 minutes；
Step 6, by the various mycopowder made above quarterly human relations series bacillus mycopowder 10-30 part, bacillus amyloliquefaciens mycopowder
20-40 part, streptomyces fimicarius mycopowder 20-40 part, beautiful streptomycete mycopowder 10-30 part, Saturn spore Trichoderma spp. mycopowder 20-40 part is yellow
Rotten acid potassium 20-60 part, aminoacid former powder 10-30 part；Middle trace element 0.1-0.2 part mixing, uniformly, as Flos Lonicerae is special micro-
Bio-bacterial manure.
2. a kind of Flos Lonicerae microbial bacterial manure special according to claim 1 is it is characterised in that described Flos Lonicerae is special
Microbial-bacterial fertilizer, the trench digging ring that applies fertilizer first is applied, and every mu of amount of application is 2-5 kg；Second fertilization time is squaring period, applies for every mu
Consumption is 1-2 kg, dilutes 100 times of -200 times of foliage-sprays；Apply foliage-spray once within every 20-30 days below, every mu of amount of application
For 1-2 kg, dilute 100 times of -200 times of foliage-sprays；Foliage-spray 2-4 time every year.
3. a kind of Flos Lonicerae microbial bacterial manure special according to claim 1 is it is characterised in that described middle trace element
By 14 parts of edta disodium, 5 parts of ferrous sulfate, 10 parts of zinc sulfate, 0.3 part of manganese chloride, 3 parts of boric acid, 0.02 part of cobaltous chloride, copper chloride
0.01 part, 0.002 part of Nickel dichloride., 3 parts of compositions of sodium molybdate.
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| CN106922349A (en) * | 2017-03-01 | 2017-07-07 | 济南大学 | A kind of method that utilization fungi promotes the accumulation of In Flower Buds of Lonicera Japonica Thunb Content of Chlorogenic Acid |
| CN109089755A (en) * | 2018-07-21 | 2018-12-28 | 邵阳市农业科学研究院 | The implantation methods of broccoli shoot vegetable |
| CN110187052A (en) * | 2019-03-08 | 2019-08-30 | 合肥学院 | It is a kind of based on the plant-growth factor of cloud computing to the analysis method of its growth effect |
| CN110256148A (en) * | 2019-06-27 | 2019-09-20 | 东莞一翔液体肥料有限公司 | A kind of diseases prevention promoting root growth microbial-bacterial fertilizer and preparation method thereof suitable for water-fertilizer integral system |
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| CN106922349A (en) * | 2017-03-01 | 2017-07-07 | 济南大学 | A kind of method that utilization fungi promotes the accumulation of In Flower Buds of Lonicera Japonica Thunb Content of Chlorogenic Acid |
| CN106922349B (en) * | 2017-03-01 | 2020-04-03 | 济南大学 | Method for promoting accumulation of chlorogenic acid in honeysuckle flower buds by using fungi |
| CN109089755A (en) * | 2018-07-21 | 2018-12-28 | 邵阳市农业科学研究院 | The implantation methods of broccoli shoot vegetable |
| CN109089755B (en) * | 2018-07-21 | 2020-12-22 | 邵阳市农业科学研究院 | Planting method of broccoli sprouting vegetable |
| CN110187052A (en) * | 2019-03-08 | 2019-08-30 | 合肥学院 | It is a kind of based on the plant-growth factor of cloud computing to the analysis method of its growth effect |
| CN110256148A (en) * | 2019-06-27 | 2019-09-20 | 东莞一翔液体肥料有限公司 | A kind of diseases prevention promoting root growth microbial-bacterial fertilizer and preparation method thereof suitable for water-fertilizer integral system |
| CN110256148B (en) * | 2019-06-27 | 2022-04-22 | 东莞一翔液体肥料有限公司 | Disease-preventing root-promoting microbial fertilizer applicable to water-fertilizer integrated system and preparation method thereof |
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