CN107125008A - A kind of method for culturing seedlings of antitumor medicinal material Herba lysimachiae capillipedis - Google Patents
A kind of method for culturing seedlings of antitumor medicinal material Herba lysimachiae capillipedis Download PDFInfo
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- CN107125008A CN107125008A CN201710475371.9A CN201710475371A CN107125008A CN 107125008 A CN107125008 A CN 107125008A CN 201710475371 A CN201710475371 A CN 201710475371A CN 107125008 A CN107125008 A CN 107125008A
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Abstract
A kind of method for culturing seedlings of antitumor medicinal material Herba lysimachiae capillipedis, comprises the following steps：Select big and full, color yellowish-brown, maturity height, without the healthy north fruit of scab, fully dried in time after harvesting, gently roll and blow away shell and premature seed, leave the seed of pure maturation health；One day prior to seeding, seed is fitted into gauze bag, soaks standby after 24h at normal temperatures with clear water or Gibberellins solution；Select in vinyl house or the good field of outdoor big Tanaka's loosing soil, crumb structure, add organic commercial fertilizer or natural detritus soil on the furrow per square meter and the soil of furrow face fully mixes leveling thoroughly, pour permeable standby；Seed is filtered dry moisture, seed, which is admixed moist fine sand and fully mixed thoroughly, to be broadcasted sowing, and nursery stage is carried out after watering, fertilizing management into transplantation of seedlings.The present invention is simple and easy to apply, is easy to grasp for medicinal herb grower, improves breeding efficiency, saves artificial, cost of land, increase economic efficiency.
Description
【Technical field】
The invention belongs to Chinese medicine cultivation technical field, and in particular to a kind of method for culturing seedlings of antitumor medicinal material Herba lysimachiae capillipedis.
【Background technology】
Herba lysimachiae capillipedis (Lysimachia capillipes Hemsl.), also known as Lysimachia sikokiana, Herba lysimachiae capillipedis, vanilla, hair handle pearl
Dish, is Primulaceae (Primulaceae) Lysimachia plant, is mainly distributed on Sichuan of China, Jiangxi, Fujian, Guangzhou, expensive
The ground such as state, Zhejiang, Henan, Taiwan.Herba lysimachiae capillipedis are as medicinal material standard, and rarely seen Jiangxi Province place is rectification of name with " spice all over the mountain " at present
Record, it is uncharged in《Chinese Pharmacopoeia》.Herba lysimachiae capillipedis are sweet, taste is flat, all herbal medicine.It is among the people to be used to treat flu cough and asthma, rheumatism
Bitterly, irregular menstruation, neurasthenia, qi-restoratives, drive ascarid, antitumor.
Zhejiang University professor Tian Jingkui therefrom isolates by the research to Herba lysimachiae capillipedis herb chemical composition and has obtained 16
Individual compound, saponin(e and flavones are the main components in the plant, and carry out antitumor external work to Herba lysimachiae capillipedis monomer saponin
Property screening, it is found that the saponin(e B contained by Herba lysimachiae capillipedis plays the role of very strong to suppress ovarian cancer cell A2780 cells (IC50<
0.1g/ml), there is good DEVELOPMENT PROSPECT.National great science and technology has been obtained by monomer anti-cancer agent of capilliposide B
The subsidy of special drug candidate, the in recent years Central China University of Science and Technology, Zhejiang University of Traditional Chinese Medicine, Hunan City University, Fujian traditional Chinese medicine
University, southeast normal university have carried out the research such as extraction, antitumor activity about the antitumor active ingredient of Herba lysimachiae capillipedis one after another.
Existing Herba lysimachiae capillipedis medicinal material is mostly wild, and fragmentary distribution, and resource reserves are very limited, it is difficult to form scale.
To ensure that the research of Herba lysimachiae capillipedis is smoothed out, since nineteen ninety-five, the Longyan institute of agricultural sciences takes the lead in carrying out Herba lysimachiae capillipedis in the whole nation
Wild domestication, seedling breeding, the research of GAP demonstration plants, have therefrom grasped suitable seedling-raising technique, are large area standardization from now on
Plant Herba lysimachiae capillipedis production and high quality seedling, scientific basis and technological guidance are provided.
Related data shows that Jiangsu Agri-Animal Husbandry Vocational College Jiang Zong celebratings et al. have studied a kind of Herba lysimachiae capillipedis cuttage and seedling culture
Method, 93.1% is up to by this method cuttage seeding rooting rate, and germination rate ensures that Herba lysimachiae capillipedis effectively medicinal contain up to 83.7
Amount, but the report applied to production is there are no at present.
Not yet there is the report of Herba lysimachiae capillipedis seed seedling-raising at present, existing research is all done by the Longyan institute of agricultural sciences, part
Medicinal herb grower also has the mode for taking cuttage to carry out numerous seedling, but the numerous seedling of cuttage has following shortcoming：1st, skewer bar source is difficult, line of breeding
Number is small, and every mu of numerous seedling is no more than 30,000；2nd, effort, human cost are high during labor；3rd, field is taken many, rent cost height；4th, cuttage seeding
Belong to vegetative propagation means, it is impossible to avoid seedling band poison.
【The content of the invention】
The technical problem to be solved in the present invention is to provide a kind of nursery of antitumor medicinal material Herba lysimachiae capillipedis simple and easy to apply
Method.
What the present invention was realized in：
A kind of method for culturing seedlings of antitumor medicinal material Herba lysimachiae capillipedis, it is characterised in that：Comprise the following steps：
Step one：The selection of seed：
The high kind of anti-tumor active ingredient content is selected in the eugonic Herba lysimachiae capillipedis planted in bamboo grove, in every
Year carries out the harvesting of north fruit to early November by the end of October, selects big and full, color yellowish-brown, maturity height, without scab
Healthy north fruit；Fully dried in time after harvesting, gently roll and blow away shell and premature seed, leave it is pure into
Ripe healthy seed, seed parameter is：Germination percentage >=75%, cleanliness >=90%, mass of 1000 kernel >=0.2g, moisture≤10%；
Step 2：The processing of seed：
One day prior to seeding, the seed chosen in step one is fitted into gauze bag, it is red with clear water or 150-450ppm
Mycin solution soaks standby after 24h at normal temperatures；
Step 3：The processing in seedbed：
Select in vinyl house or the good field of outdoor big Tanaka's loosing soil, crumb structure, Mu Shi composite fertilizers 30-
60kg, utilizes small ridging machine ridging, row spacing 90-110m, the high 20-30cm in ridge, in the final stage of whole furrow, the furrow per square meter
The soil for adding 3-6kg organic commercial fertilizer or natural detritus soil and furrow face 5-10cm fully mixes leveling thoroughly, pours permeable standby；
Step 4：Sowing：
In mid or late November, the seed handled well in step 2 is filtered dry moisture, 10-20kg tide is admixed per 50g seeds
Wet fine sand, which is fully mixed thoroughly, to be broadcasted sowing, every mu of sowing quantity 100-200g；
Furrow face after planting is squirted with sprayer, plastics Small plastic shed is put up and carries out heat and moisture preserving；
Step 5：Nursery period management：
Before emerging, Small plastic shed was opened every 10 days and is aerated ventilative and waters；According to weather condition after emerging, sunlight is strong
Shed two ends are opened when strong breathe freely, temperature is not higher than 30 DEG C in holding shed, control humidity in 50%-60% or so, often
Combined to water every 10 days and applied fertilizer, pour the urea for applying 0.8%-1%, spray a small amount of clear water after fertilising in time；Every 30 days
The removing of a weeds is carried out, by the way of manually pulling out；
Step 6：Into transplantation of seedlings：
Transplanted in the middle ten days and the last ten days in March next year, pour permeable at dusk dialling seedling the previous day, as far as possible with soil when lifting seedlings.
The advantage of the invention is that：1st, the Herba lysimachiae capillipedis medicinal material that the seedling that method for culturing seedlings of the invention is cultivated is obtained is anti-swollen
Knurl composition (saponin(e B) content is up to more than 3%, hence it is evident that antineoplastic component (saponin(e B) content in wild Herba lysimachiae capillipedis medicinal material.
2nd, method for culturing seedlings of the invention is simple and easy to apply, is easy to grasp for medicinal herb grower.Improve breeding efficiency, save artificial, cost of land, improve
Economic benefit.3rd, the Herba lysimachiae capillipedis seedling uniformity that method for culturing seedlings of the invention is produced is high, and health is without virus, breeding coefficient
Height, every mu can breed the seedling of seedling 300,000, and the output value is up to 30,000 yuan, remarkable in economical benefits.
【Embodiment】
A kind of method for culturing seedlings of antitumor medicinal material Herba lysimachiae capillipedis, comprises the following steps：
Step one：The selection of seed：
The high kind of anti-tumor active ingredient content is selected in the eugonic Herba lysimachiae capillipedis planted in bamboo grove, in 11
The first tenday period of a month moon carry out the harvesting of north fruit, select big and full, color yellowish-brown, maturity height, without the healthy north fruit of scab；Harvesting
Fully dried in time afterwards, gently roll and blow away shell and premature seed, leave the seed of pure maturation health,
Seed parameter is：Germination percentage >=75%, cleanliness >=90%, mass of 1000 kernel >=0.2g, moisture≤10%；
Step 2：The processing of seed：
One day prior to seeding, the seed chosen in step one is fitted into gauze bag, with clear water or 300ppm gibberellin
Solution soaks standby after 24h at normal temperatures；
Step 3：The processing in seedbed：
Select in vinyl house or the good field of outdoor big Tanaka's loosing soil, crumb structure, Mu Shi composite fertilizers 50kg,
NPK ratios are 20：15：15, using small ridging machine ridging, row spacing 100m, the high 20-30cm in ridge, in the final stage of whole furrow, often
The soil that 5kg organic commercial fertilizer or natural detritus soil and furrow face 8cm are added on the furrow of square meter fully mixes leveling thoroughly, pours permeable standby
With；
Step 4：Sowing：
In mid or late November, the seed handled well in step 2 is filtered dry moisture, the humidity that 15kg is admixed per 50g seeds is thin
Sand, which is fully mixed thoroughly, to be broadcasted sowing, every mu of sowing quantity 150g；
Furrow face after planting is squirted with sprayer, plastics Small plastic shed is put up and carries out heat and moisture preserving；
Step 5：Nursery period management：
Before emerging, Small plastic shed was opened every 10 days and is aerated ventilative and waters；According to weather condition after emerging, sunlight is strong
Shed two ends are opened when strong breathe freely, temperature is not higher than 30 DEG C in holding shed, control humidity in 50%-60% or so, often
Combined to water every 10 days and applied fertilizer, pour the urea for applying 0.8%-1%, spray a small amount of clear water after fertilising in time；Every 30 days
The removing of a weeds is carried out, by the way of artificial group is removed；
Step 6：Into transplantation of seedlings：
Transplanted in the middle ten days and the last ten days in March next year, pour permeable at dusk dialling seedling the previous day, band soil of being tried one's best when dialling seedling.
Two test to illustrate beneficial effects of the present invention below：
Experiment one：
1.1 test material
Seed is collected from the Herba lysimachiae capillipedis plant that wild and former artificial growth leaves, selection grain is full greatly, and maturity is good
Seed.
1.2 test method
Experiment sets three factors, respectively A different times (A1：In October, 2009, A2：In January, 2010, A3：2010 2
Month three times), B different modes (B1：Earthing, B2：Not earthing), the different seedbed (C1 of C：Vegetable garden soil, C2：Matrix ﹤ turfy soils
+ yellow mud ﹥, C3：Natural detritus soil) sowing test has been carried out, totally 18 processing, 100 seeds of each processing sowing, 3 repetitions.
Continuous observation is emerged situation in after planting 60 days, record seedling-growing time, number of emerging.
Table one：The influence of different sowing times, different modes, different seedbeds to germination
2 results and analysis
2.1 as can be seen from Table I, and the average germination percentage respectively handled has great difference, and each factor is fragrant to thin stalk
Grass seed sowing germination percentage influence very different, highest germination percentage is the 78.67% of processing A1B2C3, and minimum germination percentage is
The 9.67% of A3B1C2.
A (different sowing times) influences little to the germination percentage of seed in 2.2 3 factors；B (different modes) earthing
Whether conclusive effect is existed to the germination percentage of seed, the processing percentage of seedgermination of earthing is extremely low；C (different seedbeds) is right
The influence of percentage of seedgermination is also very big, wherein the germination of natural detritus soil optimum seed, and matrix<Turfy soil+yellow mud>It is uncomfortable
Preferably as the sowing media of Herba lysimachiae capillipedis.
Experiment two：
1 materials and methods
1.1 material
This is tested is carried out on November 29th, 2011 in room tested by Longyan's agricultural sciences, material source in
The Herba lysimachiae capillipedis seed harvested at the beginning of 11 months 2011 in Longyan rivers and mountains town Qian Cun villages.
1.2 method
1.2.1 various concentrations gibberellin (GA3) handles the influence to germination
Randomly select for planting experimentally 21 parts of son, 100 every part, be fitted into vial.Seed is handled as follows：Processing 1
24h is soaked with 150ppm gibberellin (GA3) solution；The 300ppm of processing 2 gibberellin (GA3) solution immersion 24h；Processing 3
24h is soaked with 450ppm gibberellin (GA3) solution；The 600ppm of processing 4 gibberellin (GA3) solution immersion 24h；Processing 5
24h is soaked with 750ppm gibberellin (GA3) solution；The 900ppm of processing 6 gibberellin (GA3) solution immersion 24h；Processing 7
With soaking 24h in clear water；The control of processing 8 (not soaking seed).8 kinds of processing of the above, each 3 repetitions of processing.Will germination culture used
Ware clean, above the metafiltration paper of pad one, often processing broadcast 100 seeds, be uniformly placed on culture dish filter paper respectively.It is placed in 25 DEG C of light
According in incubator.Seed sprouting situation of observation, and keep the humidity in culture dish daily.
The calculating germination percentage (%) of germination percentage=chitting piece number/experiment seed number × 100
1.2.2 influence of the different disposal to seed sprouting and seedling early growth
Randomly select for planting experimentally 8 parts of son, 100 every part, carry out after 8 kinds of above-mentioned different disposals, November 29 was seeded in
In seedling culture hole plate equipped with matrix (turfy soil+fine earth), respectively 100 seeds of processing are broadcast under 2 disks, normal temperature disposed within, keep one
Determine humidity, observe emerging and seedling early growth situation for different disposal seed.
2 results and analysis
Influence of 2.1 various concentrations gibberellin (GA3) processing to germination
Herba lysimachiae capillipedis seed is after November 29,5d started sprouting in 25 DEG C of illumination boxs, every 2 days statistics not
With the germinative number (being shown in Table 1) of processing.
Table two：Influence of various concentrations gibberellin (GA3) processing to germination
As can be seen from Table II, after being handled with (150-900ppm) GA3 and clear water that different disposal is various concentrations
Promote Herba lysimachiae capillipedis germination, the seed of various concentrations GA3 processing reaches germination peak the 15th day (December 14), hereafter
The germinative number of GA3 processing is not further added by；Clear water and the seed of control treatment are increased slightly with the extension germinative number of processing time.
In above-mentioned 8 different disposals, the germination percentage of the seed of gibberellin processing is higher than the germination percentage that clear water is handled, wherein with
Preferably, 15 days germination percentage highests, reach 94% to 450ppm GA3 treatment effects after processing, next to that the hair of 300ppm GA3 processing
Bud rate reaches 90%, and the germination percentage of the gibberellin processing seed of both concentration reaches more than 90%, 150ppm GA3 processing
Germination percentage be 87%, 600ppm GA3 processing germination percentage be 80%, 750ppm GA3 handle germination percentage be 86%,
The germination percentage of 900ppm GA3 processing is 82%, and the germination percentage of clear water processing is 72%, and the germination percentage of control (not soaking seed) is most
Low, the germination percentage of the 15th day is only 16%.Illustrate that gibberellin processing germination is better than clear water processing seed germination effects, it is red mould
Element processing seed is best with 450ppm concentration.
Influence of 2.2 different disposals to seed sprouting and seedling early growth
The Herba lysimachiae capillipedis seed of different disposal is after November 29 seeding and seedling raising hole tray, to (sowing in 1 day 2 months of Second Year
60 days afterwards) statistics emergence rate, April 20 (after planting 140 days), seedling early growth situation was recorded in observation, was shown in Table three.
Table three：Influence of the different disposal to seed sprouting and seedling early growth
As can be seen from Table III, different disposal seed is sowed at after matrix 60 days, and what 3 (450ppm) GA3 of processing were handled emerges
Rate is up to 88%, and the seeding ratio of processing 7 clear water processing and the emergence rate difference that other various concentrations GA3 are handled are little,
Compare the seeding ratio do not soaked seed minimum, only 32%.Different disposal seed is sowed at after matrix 140 days, and 6 kinds of various concentrations are red
The equal growing way of seedling of mycin processing is uneven, and height of seedling 2-11 ㎝, 2-10 piece leaves have obvious excessive growth, aggregate performance seedling growing way is weak；Control
Seedling also show long potential difference, seedling is weak；And the equal growing way of seedling of clear water processing is neat, height of seedling 6-8 ㎝, 7-10 piece leaves, seedling is sturdy.
Although it is possible thereby to 12 hundred will be higher by than the seeding ratio that clear water is handled by illustrating the seed highest emergence rate of gibberellin processing
Branch, but the seed of clear water processing is neat in the growth performance growing way of later stage seedling, and seedling is sturdy, and the seed of gibberellin processing is rear
It is uneven that the growth of phase seedling shows growing way, has obvious excessive growth, seedling is weak.
It can be drawn from experiment two：The vernalization processing of Herba lysimachiae capillipedis seed, gibberellin processing seed and clear water processing seed
Germination can be promoted.Various concentrations gibberellin processing seed is best with 450ppm concentration in this experiment, germinates within 15 days
Rate can reach 94%, more preferable than clear water processing seed germination effects.By observing different disposal to seed sprouting and seedling early growth
Influence can draw, the seed of clear water processing being eager to excel than gibberellin processing in terms of the growth potential of seedling, performance growing way is neat,
Seedling is sturdy.So while gibberellin handles seed handles higher on germination percentage and emergence rate than clear water, but in production
The method that Herba lysimachiae capillipedis seed 24h is soaked with clear water can be used, it is both simple and convenient and good to the seedling early growth of Herba lysimachiae capillipedis
Good facilitation.
Table four：Seed seedling-raising of the present invention is contrasted with cuttage and seedling culture
Antineoplastic component (saponin(e B) content for the Herba lysimachiae capillipedis medicinal material that the seedling that the method for culturing seedlings of the present invention is cultivated is obtained is high
Up to more than 3%, hence it is evident that antineoplastic component (saponin(e B) content in wild Herba lysimachiae capillipedis medicinal material.The method for culturing seedlings letter of the present invention
It is single easy, it is easy to grasp for medicinal herb grower.Improve breeding efficiency, save artificial, cost of land, increase economic efficiency.The present invention's educates
The Herba lysimachiae capillipedis seedling uniformity that seedling-growing method is produced is high, and health is without virus, and breeding coefficient is high, and every mu can breed seedling 300,000
Seedling, the output value is up to 30,000 yuan, remarkable in economical benefits.
The preferable implementation use-case of the present invention is the foregoing is only, is not intended to limit the scope of the present invention.It is all
Within the spirit and principles in the present invention, any modification, equivalent substitution and improvement for being made etc., should be included in the present invention's
Within protection domain.
Claims (1)
1. a kind of method for culturing seedlings of antitumor medicinal material Herba lysimachiae capillipedis, it is characterised in that：Comprise the following steps：
Step one：The selection of seed：
The high kind of anti-tumor active ingredient content is selected in the eugonic Herba lysimachiae capillipedis planted in bamboo grove, in annual 10
The end of month to early November carries out the harvesting of north fruit, selects big and full, color yellowish-brown, maturity height, without the health of scab
North fruit；Fully dried in time after harvesting, gently roll and blow away shell and premature seed, left pure maturation and be good for
The seed of health, seed parameter is：Germination percentage >=75%, cleanliness >=90%, mass of 1000 kernel >=0.2g, moisture≤10%；
Step 2：The processing of seed：
One day prior to seeding, the seed chosen in step one is fitted into gauze bag, with clear water or 150-450ppm gibberellin
Solution soaks standby after 24h at normal temperatures；
Step 3：The processing in seedbed：
Select in vinyl house or the good field of outdoor big Tanaka's loosing soil, crumb structure, Mu Shi composite fertilizers 30-60kg, profit
With small ridging machine ridging, row spacing 90-110m, the high 20-30cm in ridge add 3- in the final stage of whole furrow, the furrow per square meter
The soil of 6kg organic commercial fertilizer or natural detritus soil and furrow face 5-10cm fully mixes leveling thoroughly, pours permeable standby；
Step 4：Sowing：
In mid or late November, the seed handled well in step 2 is filtered dry moisture, the humidity that 10-20kg is admixed per 50g seeds is thin
Sand, which is fully mixed thoroughly, to be broadcasted sowing, every mu of sowing quantity 100-200g；
Furrow face after planting is squirted with sprayer, plastics Small plastic shed is put up and carries out heat and moisture preserving；
Step 5：Nursery period management：
Before emerging, Small plastic shed was opened every 10 days and is aerated ventilative and waters；According to weather condition after emerging, during sunlight strong
Open shed two ends to be breathed freely, keep temperature in shed to be not higher than 30 DEG C, control humidity is in 50%-60% or so, every 10
It combines to water and applied fertilizer, and pours the urea for applying 0.8%-1%, sprays a small amount of clear water after fertilising in time；Carried out every 30 days
The removing of weeds, by the way of manually pulling out；
Step 6：Into transplantation of seedlings：
Transplanted in the middle ten days and the last ten days in March next year, pour permeable at dusk dialling seedling the previous day, as far as possible with soil when lifting seedlings.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107466662A (en) * | 2017-09-21 | 2017-12-15 | 龙岩市农业科学研究所 | A kind of underwood planting method of Herba lysimachiae capillipedis |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102668981A (en) * | 2012-05-17 | 2012-09-19 | 云南自然谷生物开发有限公司 | Valeriana jatamansi jones breeding method |
| CN104170639A (en) * | 2014-09-04 | 2014-12-03 | 张汶洲 | Cultivating method for medicinal and edible dual-purpose high-calcium loosestrife |
| CN105409570A (en) * | 2015-12-25 | 2016-03-23 | 广西南岜仔科技有限公司 | Planting method for soil-cultured lysimachia foenum-graecum hance |
| CN105940921A (en) * | 2016-05-23 | 2016-09-21 | 江苏农牧科技职业学院 | Seedling raising method of lysimachia capillipes by cutting |
-
2017
- 2017-06-21 CN CN201710475371.9A patent/CN107125008A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102668981A (en) * | 2012-05-17 | 2012-09-19 | 云南自然谷生物开发有限公司 | Valeriana jatamansi jones breeding method |
| CN104170639A (en) * | 2014-09-04 | 2014-12-03 | 张汶洲 | Cultivating method for medicinal and edible dual-purpose high-calcium loosestrife |
| CN105409570A (en) * | 2015-12-25 | 2016-03-23 | 广西南岜仔科技有限公司 | Planting method for soil-cultured lysimachia foenum-graecum hance |
| CN105940921A (en) * | 2016-05-23 | 2016-09-21 | 江苏农牧科技职业学院 | Seedling raising method of lysimachia capillipes by cutting |
Non-Patent Citations (3)
| Title |
|---|
| 中国大百科全书总编辑委员会: "《中国大百科全书生物I》", 30 September 2002 * |
| 虞天龙，郭伟达: "抗肿瘤药材-细梗香草规范化种植（GAP）初探", 《中国野生植物资源》 * |
| 韩世栋等: "《辣椒生产技术百问百答》", 31 January 2006 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107466662A (en) * | 2017-09-21 | 2017-12-15 | 龙岩市农业科学研究所 | A kind of underwood planting method of Herba lysimachiae capillipedis |
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Application publication date: 20170905 |