CN107089868A - A kind of preparation method for being conducive to preventing and treating the microbial manure of sugar orange anthracnose - Google Patents
A kind of preparation method for being conducive to preventing and treating the microbial manure of sugar orange anthracnose Download PDFInfo
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- CN107089868A CN107089868A CN201710402214.5A CN201710402214A CN107089868A CN 107089868 A CN107089868 A CN 107089868A CN 201710402214 A CN201710402214 A CN 201710402214A CN 107089868 A CN107089868 A CN 107089868A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/10—Solid or semi-solid fertilisers, e.g. powders
- C05G5/12—Granules or flakes
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D5/00—Fertilisers containing magnesium
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
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- General Chemical & Material Sciences (AREA)
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- Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Inorganic Chemistry (AREA)
- Fertilizers (AREA)
Abstract
The invention provides a kind of preparation method for being conducive to preventing and treating the microbial manure of sugar orange anthracnose, the main component of the fertilizer is become thoroughly decomposed material, vermiculite, azophoska and water including bacteria residue；Vermiculite is added in fertilizer by the microbial manure, has slow-release function to the various nutrients especially trace element in fertilizer, and nutrition can be provided to the nutrition that sugar orange plants whole growth cycle；Special mixed bacteria is used in the stage of becoming thoroughly decomposed of bacteria residue, effect of not only becoming thoroughly decomposed is good, and than conventional method, the time shortens more than half, and cost is low, and effect is good；Other raw materials such as trace element need not be added in addition to bacteria residue becomes thoroughly decomposed material, vermiculite, traditional azophoska and water in the formula of microbial manure, cost is low.In summary, fertilizer of the invention can not only cost it is low, bacteria residue utilization rate is high, and notable to the prevention effect of sugar orange anthracnose.
Description
Technical field
The present invention relates to technical field of fertilizers, it particularly relates to a kind of micro- life for being conducive to preventing and treating sugar orange anthracnose
The preparation method of thing fertilizer.
Background technology
With the development of mushroom industry, the quantity of bacteria residue is sharply increased, because bacteria residue does not obtain environmental protection treatment, arbitrarily
Abandon, not only cause the wasting of resources, while the easy moldy metamorphism of bacteria residue, contaminated land and water source, mycotic spore around with the wind
Float, compromise the healthy of people.The miscellaneous bacteria of bacteria residue mainly has Fusarium, mould, trichoderma etc..It can cause exogenous mistake
Quick property pulmonary alveolitis bronchioli terminales.How to dissolve and put the Tough questions in face of scientific and technical personnel using these bacteria residues.
Bacteria residue contains numerous available nutrients.Such as crude protein, crude fat, crude fibre, trace element, also have many lifes
Active substances, such as polysaccharide, organic acid, enzyme, phenolic substance chemical substance.
Making organic fertilizer using bacteria residue can increase soil fertility, improves soil physical and chemical property, enhancing retention ability and permeability
It is a kind of good bacterial manure with the growth of stimulation rhizosphere nitrogen-fixing microorganism.
But in traditional technique, the speed of becoming thoroughly decomposed of bacteria residue is especially slow, as fertilizer, to improve the efficiency of becoming thoroughly decomposed of bacteria residue,
It is necessary to study efficiently become thoroughly decomposed technique and its application in fertilizer of a kind of bacteria residue.
The content of the invention
In view of this, it is an object of the invention to provide a kind of system for being conducive to preventing and treating the microbial manure of sugar orange anthracnose
Preparation Method, this method substantially increases the efficiency of becoming thoroughly decomposed of bacteria residue.
A kind of preparation method for being conducive to preventing and treating the microbial manure of sugar orange anthracnose, comprises the following steps：
A, kluyveromyces marxianus bacterium, bacillusmusilaginosiengineering and bread mold done into strain, cultivated through inclined-plane and shaking flask,
Enter back into ferment in liquid fermentation fermentor after seed tank culture and produce liquid bacteria sample；
B, by freeze-drying powdered bacterium sample is made in liquid bacteria sample；
C, graphene is added in powdered bacterium sample, carries out ball milling, control the particle diameter of powdered bacterium sample for 10-50 μm,
Obtain micron powder shape bacterium sample；
D, micron powder shape bacterium sample is mixed with edible fungi residue after, added water carry out compost maturity, and ultraviolet disinfection is obtained
Become thoroughly decomposed material to bacteria residue；
E, vermiculite is put into pulverizer, is crushed to powdery；
F, by bacteria residue become thoroughly decomposed material with vermiculite be stirring evenly and then adding into remaining raw material, stir；
G, drying, granulation, obtain the microbial manure for being conducive to preventing and treating sugar orange anthracnose.
It is preferred that, described edible fungi residue is mushroom or the bacteria residue of asparagus.
It is preferred that, in described step C, the addition of kluyveromyces marxianus bacterium, bacillusmusilaginosiengineering and bread mold
Measure as 108cfu/g、109Cfu/g and 104cfu/g。
It is preferred that, in described step D, the time control of compost maturity shortened near at 3-4 days than conventional method
Half the time.
It is preferred that, in described step F, the particle diameter of vermiculite is respectively less than 1mm.
It is preferred that, the described microbial manure for being conducive to preventing and treating sugar orange anthracnose, including following raw material：Bacteria residue becomes thoroughly decomposed
Material, vermiculite, azophoska and water.
It is preferred that, the described microbial manure for being conducive to preventing and treating sugar orange anthracnose, including following percentage by weight
Raw material：Bacteria residue, which becomes thoroughly decomposed, expects 60-75%, vermiculite 3-5%, azophoska 8-15% and water surplus.
Bread mold (Rhizopus nigricans) is one kind of fungi, belongs to the rhizopus of Eumycota Zygomycotina,
Mycelia is without every often using its saccharification, such as the main bacteria seed in koji is exactly bread mold.
Bread mold, is also named bread mold, widely distributed, often colonizes on bread and daily bread, or mixes in culture medium,
The rotting in transport and storage such as gourd, fruit and vegetable and the soft corruption of sweet potato are all associated, and mycelium secrets out of pectase, decomposes
The cell membrane of host, infection site can rot to form blackspot quickly.Bread mold (ATCC6227b) is often made in current fermentation industry
Microorganism fungus kind.The optimum growth temperature of bread mold is about 28 DEG C, the not regrowth more than 32 DEG C.
Bread mold is a kind of saprophytic in the fungi on bread, steamed bun and rice.Internally the mycelia of matrix is stretched into growth, cries
Do " vegetative hyphae "；To the mycelia of outgrowth outside matrix, it is called " upright mycelia ".It is upward at rhizoid during vegetative propagation
Produce upright sporangiophore, ultimate swelling produces spore in capsule, is in black when ripe into radial sporangium.Spore one
As fly away in atmosphere, fall in suitable matrix sprout form new mycelium.A small amount of bread mold is added in the present invention just
It is to utilize its saccharification, accelerates lignin and coarse-fibred degradation speed in bacteria residue, but bread mold addition is too high, although
The degradation speed of bacteria residue is accelerated, it can also cause the nutriment in bacteria residue is largely lost in, therefore the selection order of magnitude is
104Cfu/g is more suitable.
Vermiculite be outside a kind of secondary metamorphic mineral of water aluminosilicate containing magnesium of layer structure, raw ore like mica, generally by
Black (gold) mica is formed through hydrothermal alteration effect or weathering, and curved in scratching during because of its heated dehydration expansion, form exactly likes leech, therefore
Claim vermiculite.Fabric and raw-vermiculite piece is after high-temperature roasting, and its volume energy expands rapidly several times to tens times, and the vermiculite after volumetric expansion is just
It is expanded vermiculite.Vermiculite can be used as soil conditioner, because it has good cation exchange and adsorptivity, can improve soil
The structure of earth, water storage soil moisture conservation, improves the gas permeability and the property of water-bearing of soil, acid soil is changed into neutral soil；Vermiculite can also rise
To cushioning effect, the rapid change of pH value is hindered, fertilizer is slowly discharged in plant growth medium, and allow to make slightly excessively
Plant is safe from harm with fertilizer；K, Mg, Ca, Fe for itself containing can be also provided to crop for vermiculite and micro Mn, Cu,
The elements such as Zn.Water imbibition, cation exchange and the properties of chemical composition of vermiculite, make it play fertilizer conservation, water conservation, water storage, ventilative
With the multiple action such as mineral fertilizer.Experiment shows：By in 0.5-1% expanded vermiculite incorporation composite fertilizer, crop yield can be made
Improve 15-20%.
The preparation method provided by the present invention for being conducive to preventing and treating the microbial manure of sugar orange anthracnose, one is by vermiculite
It is added in fertilizer, has slow-release function to the various nutrients especially trace element in fertilizer, whole is planted to sugar orange
The nutrition of growth cycle can provide nutrition；Two be to use special mixed bacteria, effect of not only becoming thoroughly decomposed in the stage of becoming thoroughly decomposed of bacteria residue
It is really good, and than conventional method, the time shortens more than half, and cost is low, and effect is good；Three be microbial manure formula in except
Bacteria residue, which becomes thoroughly decomposed, need not add other raw materials such as trace element outside material, vermiculite, traditional azophoska and water, and cost is low.To sum up
It is described, fertilizer of the invention can not only cost it is low, bacteria residue utilization rate is high, and the prevention effect of sugar orange anthracnose is shown
Write, advantageously reduce the usage amount of agricultural chemicals, improve the quality of sugar orange.
Embodiment
Following examples, to further labor of the invention：
Embodiment 1
A kind of preparation method for being added with the microbial manure for being beneficial to prevent and treat sugar orange anthracnose, enters as steps described below
OK：
A, kluyveromyces marxianus bacterium, bacillusmusilaginosiengineering and bread mold done into strain, cultivated through inclined-plane and shaking flask,
Enter back into ferment in liquid fermentation fermentor after seed tank culture and produce liquid bacteria sample；
B, by freeze-drying powdered bacterium sample is made in liquid bacteria sample；
C, graphene is added in powdered bacterium sample, carries out ball milling, control the particle diameter of powdered bacterium sample for 10-50 μm,
Obtain micron powder shape bacterium sample；
D, micron powder shape bacterium sample is mixed with edible fungi residue after, added water carry out compost maturity, and ultraviolet disinfection is obtained
Become thoroughly decomposed material to bacteria residue；
E, vermiculite is put into pulverizer, is crushed to powdery；
F, by bacteria residue become thoroughly decomposed material with vermiculite be stirring evenly and then adding into remaining raw material, stir；
G, drying, granulation, obtain the microbial manure for being conducive to preventing and treating sugar orange anthracnose.
In described step C, the addition of kluyveromyces marxianus bacterium, bacillusmusilaginosiengineering and bread mold is
108cfu/g、109Cfu/g and 104cfu/g。
In described step D, the time control of compost maturity was at 80 hours.
In described step F, the particle diameter of vermiculite is respectively less than 1mm.
The described microbial manure for being conducive to preventing and treating sugar orange anthracnose, includes the raw material of following percentage by weight：Bacterium
Slag becomes thoroughly decomposed material 72%, vermiculite 4%, azophoska 10% and water surplus.
Described edible fungi residue is the bacteria residue of mushroom.
Embodiment 2
A kind of preparation method for being added with the microbial manure for being beneficial to prevent and treat sugar orange anthracnose, enters as steps described below
OK：
A, kluyveromyces marxianus bacterium, bacillusmusilaginosiengineering and bread mold done into strain, cultivated through inclined-plane and shaking flask,
Enter back into ferment in liquid fermentation fermentor after seed tank culture and produce liquid bacteria sample；
B, by freeze-drying powdered bacterium sample is made in liquid bacteria sample；
C, graphene is added in powdered bacterium sample, carries out ball milling, control the particle diameter of powdered bacterium sample for 10-50 μm,
Obtain micron powder shape bacterium sample；
D, micron powder shape bacterium sample is mixed with edible fungi residue after, added water carry out compost maturity, and ultraviolet disinfection is obtained
Become thoroughly decomposed material to bacteria residue；
E, vermiculite is put into pulverizer, is crushed to powdery；
F, by bacteria residue become thoroughly decomposed material with vermiculite be stirring evenly and then adding into remaining raw material, stir；
G, drying, granulation, obtain the microbial manure for being conducive to preventing and treating sugar orange anthracnose.
In described step C, the addition of kluyveromyces marxianus bacterium, bacillusmusilaginosiengineering and bread mold is
108cfu/g、109Cfu/g and 104cfu/g。
In described step D, the time control of compost maturity was at 72 hours.
In described step F, the particle diameter of vermiculite is respectively less than 1mm.
The described microbial manure for being conducive to preventing and treating sugar orange anthracnose, including following raw material：Bacteria residue becomes thoroughly decomposed material, leech
Stone, azophoska and water.
The described microbial manure for being conducive to preventing and treating sugar orange anthracnose, includes the raw material of following percentage by weight：Bacterium
Slag becomes thoroughly decomposed material 60%, vermiculite 5%, azophoska 15% and water surplus.
Described edible fungi residue is the bacteria residue of asparagus.
Embodiment 3
A kind of preparation method for being added with the microbial manure for being beneficial to prevent and treat sugar orange anthracnose, enters as steps described below
OK：
A, kluyveromyces marxianus bacterium, bacillusmusilaginosiengineering and bread mold done into strain, cultivated through inclined-plane and shaking flask,
Enter back into ferment in liquid fermentation fermentor after seed tank culture and produce liquid bacteria sample；
B, by freeze-drying powdered bacterium sample is made in liquid bacteria sample；
C, graphene is added in powdered bacterium sample, carries out ball milling, control the particle diameter of powdered bacterium sample for 10-50 μm,
Obtain micron powder shape bacterium sample；
D, micron powder shape bacterium sample is mixed with edible fungi residue after, added water carry out compost maturity, and ultraviolet disinfection is obtained
Become thoroughly decomposed material to bacteria residue；
E, vermiculite is put into pulverizer, is crushed to powdery；
F, by bacteria residue become thoroughly decomposed material with vermiculite be stirring evenly and then adding into remaining raw material, stir；
G, drying, granulation, obtain the microbial manure for being conducive to preventing and treating sugar orange anthracnose.
In described step C, the addition of kluyveromyces marxianus bacterium, bacillusmusilaginosiengineering and bread mold is
108cfu/g、109Cfu/g and 104cfu/g。
In described step D, the time control of compost maturity was at 84 hours.
In described step F, the particle diameter of vermiculite is respectively less than 1mm.
The described microbial manure for being conducive to preventing and treating sugar orange anthracnose, including following raw material：Bacteria residue becomes thoroughly decomposed material, leech
Stone, azophoska and water.
The described microbial manure for being conducive to preventing and treating sugar orange anthracnose, includes the raw material of following percentage by weight：Bacterium
Slag becomes thoroughly decomposed material 75%, vermiculite 3%, azophoska 8% and water surplus.
Described edible fungi residue is the mixing bacteria residue of mushroom and asparagus.
Comparative example 1
Bread mold in the strain of embodiment 1 is removed, remaining preparation condition is constant.
Bacteria residue in embodiment 1-3 and the microbial manure of comparative example 1 is become thoroughly decomposed below material the compost maturity time and application
After sugar orange plantation, the incidence of disease of sugar orange anthracnose is detected, following data are obtained：
| The compost maturity time (hour) | Anthracnose incidence of disease % | |
| Embodiment 1 | 80 | 3 |
| Embodiment 2 | 72 | 4 |
| Embodiment 3 | 84 | 4 |
| Comparative example 1 | 220 | 12 |
By above test data it is recognised that due to not adding bread mold, the compost maturity time of microbial manure is notable
Increase, while the microbial manure of the present invention can significantly reduce the incidence of disease of sugar orange anthracnose.
The foregoing is only a preferred embodiment of the present invention, but protection scope of the present invention be not limited thereto,
Any one skilled in the art the invention discloses technical scope in, technique according to the invention scheme and its
Inventive concept is subject to equivalent substitution or change, should all be included within the scope of the present invention.
Claims (7)
1. a kind of preparation method for being conducive to preventing and treating the microbial manure of sugar orange anthracnose, it is characterised in that including following step
Suddenly：
A, kluyveromyces marxianus bacterium, bacillusmusilaginosiengineering and bread mold done into strain, cultivated through inclined-plane and shaking flask, then enter
Enter to ferment in liquid fermentation fermentor after seed tank culture and produce liquid bacteria sample；
B, by freeze-drying powdered bacterium sample is made in liquid bacteria sample；
C, graphene is added in powdered bacterium sample, carries out ball milling, control the particle diameter of powdered bacterium sample for 10-50 μm, obtain
Micron powder shape bacterium sample；
D, micron powder shape bacterium sample is mixed with edible fungi residue after, add water carry out compost maturity, and ultraviolet disinfection obtains bacterium
Slag becomes thoroughly decomposed material；
E, vermiculite is put into pulverizer, is crushed to powdery；
F, by bacteria residue become thoroughly decomposed material with vermiculite be stirring evenly and then adding into remaining raw material, stir；
G, drying, granulation, obtain the microbial manure for being conducive to preventing and treating sugar orange anthracnose.
2. being conducive to preventing and treating the preparation method of the microbial manure of sugar orange anthracnose as claimed in claim 1, its feature exists
In in described step C, the addition of kluyveromyces marxianus bacterium, bacillusmusilaginosiengineering and bread mold is 108cfu/g、
109Cfu/g and 104cfu/g。
3. being conducive to preventing and treating the preparation method of the microbial manure of sugar orange anthracnose as claimed in claim 1, its feature exists
In in described step D, the time control of compost maturity was at 3-4 days.
4. being conducive to preventing and treating the preparation method of the microbial manure of sugar orange anthracnose as claimed in claim 1, its feature exists
In in described step F, the particle diameter of vermiculite is respectively less than 1mm.
5. being conducive to preventing and treating the preparation method of the microbial manure of sugar orange anthracnose as claimed in claim 1, its feature exists
In, the described microbial manure for being conducive to preventing and treating sugar orange anthracnose, including following raw material：Become thoroughly decomposed material, vermiculite, azophoska
And water.
6. being conducive to preventing and treating the preparation method of the microbial manure of sugar orange anthracnose as claimed in claim 5, its feature exists
In the described microbial manure for being conducive to preventing and treating sugar orange anthracnose includes the raw material of following percentage by weight：Bacteria residue becomes thoroughly decomposed
Expect 60-75%, vermiculite 3-5%, azophoska 8-15% and water surplus.
7. being conducive to preventing and treating the preparation method of the microbial manure of sugar orange anthracnose as claimed in claim 1, its feature exists
In described edible fungi residue is mushroom or the bacteria residue of asparagus.
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| CN108863595A (en) * | 2018-08-23 | 2018-11-23 | 安徽农耕年华农业发展有限公司 | A method of fertilizer is prepared using mushroom residue |
| CN109054843A (en) * | 2018-08-03 | 2018-12-21 | 北京农业生物技术研究中心 | A kind of soil conditioner and preparation method and its application as pesticide synergistic agent |
| CN112772685A (en) * | 2021-02-10 | 2021-05-11 | 乔建国 | Preparation for preventing and treating longicorn and preparation method thereof |
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