CN109485498A

CN109485498A - A kind of method of preparation and use of high organic complex microorganism flushing and spreading fertilizer

Info

Publication number: CN109485498A
Application number: CN201811440208.XA
Authority: CN
Inventors: 鲁耀雄; 张�浩
Original assignee: CHANGSHA HAOBO BIOLOGICAL TECHNOLOGY CO LTD
Current assignee: CHANGSHA HAOBO BIOLOGICAL TECHNOLOGY CO LTD
Priority date: 2018-11-29
Filing date: 2018-11-29
Publication date: 2019-03-19

Abstract

A kind of method of preparation and use of high organic complex microorganism flushing and spreading fertilizer, it is related to technical field of fertilizers.It is made of the raw material of following parts by weight: potassium fulvate, potassium nitrate, urea, potassium sulfate, complete water-soluble ammonium phosphate, potassium hydrogen phosphate, amino acid fertilizer, humic acid, peptone, concentrated biological fermentation liquid, modified biomass charcoal, microbial bacterial agent, middle microelement.The invention has the following beneficial effects: while supplementing inorganic nutrients, also supplement organic matter and functional microorganism bacterium, play adjusting structure of soil microbial community, realize the organic and inorganic effective combination with microorganism of flushing and spreading fertilizer, the generation of soil continuous cropping obstacle can be effectively relieved, the root rot disease reduced in greenhouse facility cultivation occurs, and increases yield, improves the quality of agricultural product.

Description

A kind of method of preparation and use of high organic complex microorganism flushing and spreading fertilizer

Technical field

The present invention relates to technical field of fertilizers, and in particular to a kind of preparation of high organic complex microorganism flushing and spreading fertilizer and Application method.

Background technique

With the adjustment of agricultural structure, a large amount of rural laborers work to city, and planting industry is intensive to scale Fast development, a large amount of vegetable cultivation village specializing in a certain trade, professional rich and influential family, base, cooperative society and enterprise continue to bring out, vegetable cultivation area It expands rapidly.During vegetable cultivation, tree-like big due to solanaceous crops, as a result fecund amount is big, and harvest time is long, cultural method It is simple and with a long history, it is good in economic efficiency, it is deep to be liked by farmer.Therefore, solanaceous crops cultivated area accounting is larger.

In the cultivation of the solanaceous crops such as capsicum, eggplant and tomato, due to the limitation of labor cost, farmer In order to pursue short-term benefit, using the water-fertilizer integral cultivation mode of high fertile Gao Shui, one batch of solanaceous crops, which needs to rush, applies water solubility Compound fertilizer up to ten times, under cultivation mode that is this Farmland was overused without proper attention to the maintenance of its fertility or even carrying out predatory management, cause the soil organism Content reduces, and gas permeability reduces, and the microbial activity decline of aerobism, soil-ripening is slow, causes soil acidification hardened, salination It aggravates, microelement missing in part in soil, vegetables nutritional deficiency symptom is relatively conventional, and the utilization rate of fertilizer is extremely low, and due to rule The long-term same crop of continuous cropping is accustomed in the limitation of modelling cultivation and cultivation experience, farmer or enterprise, leads to solanaceous crops continuous cropping Obstacle increasingly sharpens, and soil-borne disease occurs seriously, to increase Pesticide use amount, cause the residual of victual exceeded, reduces vegetable Vegetable matter.

The presently commercially available property the applied water soluble fertilizer that rushes is urea, potassium nitrate, potassium sulfate, water-soluble phosphoric acid monoammonium, phosphorus mostly The mixture of the chemical raw materials such as sour hydrogen dipotassium is easy dampness agglomeration, and impurity is excessive, has seriously affected the sale of Water soluble fertilizer；And And it is long-term largely using chemical fertilizer, will lead to organic fertility deficiency, nutrients ratio numerous imbalances, continuous continuous cropping in soil leads to soil Earth pest and disease damage occurs serious.Therefore, the present invention is directed to the cultivation mode of solanaceous crops water-fertilizer integral, reduces fertilizer and pesticide and applies With raising soil organic matter content prevents the generation of continuous cropping soil-borne disease, it is compound micro- to propose a kind of solanaceous crops high organic Biological defense fertilization material.

Summary of the invention

In view of the defects and deficiencies of the prior art, the present invention intends to provide a kind of high organic complex microorganisms to rush The method of preparation and use expected of applying fertilizer also supplements organic matter and functional microorganism bacterium, plays tune while supplementing inorganic nutrients Structure of soil microbial community is saved, the organic and inorganic effective combination with microorganism of flushing and spreading fertilizer is realized, soil can be effectively relieved The generation of earth continuous cropping obstacle, the root rot disease reduced in greenhouse facility cultivation occur, increase yield, improve agricultural product Quality.

To achieve the above object, the present invention is using following technical scheme: a kind of high organic complex microorganism punching fertilising Material, it is made of the raw material of following parts by weight: 30-85 parts of potassium fulvate, 5-25 parts of potassium nitrate, 1-10 parts of urea, sulfuric acid 1-10 parts of potassium, complete water-soluble ammonium phosphate 1-10 parts, 1-5 parts of potassium hydrogen phosphate, 4-10 parts of amino acid fertilizer, 1-5 parts of humic acid, peptone 0.2-2 parts, 0.2-2 parts of concentrated biological fermentation liquid, 0.2-4 parts of modified biomass charcoal, 0.2-5 parts of microbial bacterial agent, in micro member It is 0.2-2 parts plain；

The potassium fulvate and humic acid is biochemical treated water-soluble substances；The amino acid is the dynamic of commodity Material resource amino acid is using various ocean fish processing factories leftover bits and pieces, boiling pulp-water as raw material, through microbial fermentation solution and biodegradable egg White matter, after generating some new active materials, spray drying is process；The concentrated biological fermentation liquid is the liquid of commodity Bio-fermented liquid is to be generated by organic goods, materials and equipments such as cane molasses, bagasse by deep-layer liquid biofermentation technique；Described Modified biomass charcoal is husk after the carbonized substance that anaerobism burn incompletely is formed, then through ball mill ball milling at nanoscale Particle, using the material of the sieve of 500 mesh；The middle microelement is amino acid chelated calcium, magnesium sulfate, zinc sulfate, boron Sand, amino acid chelated iron, cerium nitric acid rare earth according to 1~10 part: 1~10 part: 1~5 part: 1~5 part: 0~1 part: 0 of mass ratio~ 0.1 part is mixed；The microbial bacterial agent is bacillus subtilis, bacillus licheniformis, bacillus laterosporus, yeast Bacterium, colloid bacillus cereus are according to bacterium number than 1~10 part: 1~10 part: 1~10 part: 0~5 part: 0~5 part is mixed, microorganism Effective bacterial content of microbial inoculum >=10,000,000,000 CFU/g；The complete water-soluble ammonium phosphate is monoammonium phosphate or Diammonium phosphate (DAP)；The phosphoric acid hydrogen Potassium is potassium dihydrogen phosphate or dipotassium hydrogen phosphate.

A kind of preparation method of high organic complex microorganism flushing and spreading fertilizer it comprise the steps of:

Step 1: the preparation of bacillus subtilis: the bacillus subtilis strain being stored under -4 DEG C of environment is seeded to 30 DEG C of culture 48h, picking single colonie are seeded in beef extract-peptone fluid nutrient medium on beef peptone plate, beef extract egg White peptone culture medium: beef extract 3.0g/L, peptone 10.0g/L, sodium chloride 5.0g/L, pH 7.2-7.6 are 20-35 DEG C in temperature In the environment of cultivate for 24 hours after, obtain required inoculation liquid；Inoculation liquid is inoculated in the 300L seeding tank equipped with seed culture medium, Seed culture medium: peptone 8.0-15.0g/L, yeast extract 2.0-5.0g/L, beef extract 3.0-5.0g/L, glucose 3.0- 5.0g/L, sodium chloride 2.0-5.0g/L, MgSO₄·7H₂O 0.3-0.5g/L, K₂HPO₄0.3-0.5g/L, FeCl₃0.01- 0.05g/L, connecing bacterium amount is 5-10%, is fermented after connecing bacterium, the initial pH value 6.8-7.5 of fermentation, 28-35 DEG C of temperature, revolving speed 150-250r/min, ventilation ratio are 1:0.6-1.6, and when seeding tank fermentation 30-50 is small, thalli growth is in logarithmic phase, this When the fermentation liquid in seeding tank is added to the relaying supervention ferment of the 3000L fermentor equipped with fermentation medium, fermentation medium: beautiful Rice & peanut milk 20.0-30.0g/L, beancake powder 30.0-50.0g/L, yeast powder 3.0-5.0g/L, sucrose 5.0-8.0g/L, MgSO₄· 7H₂O 0.3-0.5g/L, NaH₂PO₄0.5-1.0g/L, CaCl₂·2H₂O 0.1-0.5g/L, K₂HPO₄0.3-0.5g/L, FeCl₃0.01-0.05g/L, pH 6.5-7.8, the same seeding tank of control condition are cultivated 64-96 hours, as thallus sporulation 90- When 95%, terminates fermentation, obtain bacillus subtilis fermentation liquor；After passing it through film concentration, then middle low temperature drying, by its powder After broken, cell concentration >=10,000,000,000 CFU/g solid powders is spare；

Step 2: the preparation of bacillus licheniformis: the lichem bacillus strain being stored under -4 DEG C of environment is seeded to 30 DEG C of culture 48h, picking single colonie are seeded in beef extract-peptone fluid nutrient medium on beef peptone plate, beef extract egg White peptone culture medium: beef extract 3.0g/L, peptone 10.0g/L, sodium chloride 5.0g/L, pH 7.2-7.6 are 20-30 DEG C in temperature In the environment of cultivate for 24 hours after, obtain required inoculation liquid；Inoculation liquid is inoculated in the 300L seeding tank equipped with seed culture medium, Seed culture medium: glucose 5.0-10.0g/L, peptone 8.0-15.0g/L, yeast extract 3.0-5.0g/L, beef extract 3.0- 5.0g/L, MgSO₄·7H₂O0.3-0.5g/L,K₂HPO₄0.1-0.3g/L, connecing bacterium amount is 5-10%, is fermented after connecing bacterium, is sent out The initial pH value 6.5-7.5 of ferment, 20-45 DEG C of temperature, revolving speed 150-250r/min, ventilation ratio are 1:0.6-1.6, work as seeding tank Fermentation 30-50 hours, thalli growth is in logarithmic phase, and the fermentation liquid in seeding tank is added to equipped with fermentation medium at this time 3000L fermentor relays supervention ferment, fermentation medium: corn pulp 15.0-30.0g/L, beancake powder 20.0-30.0g/L, yeast powder 3.0-5.0g/L sucrose 3-5g/L, MgSO₄·7H₂O 0.3-0.5g/L, NaH₂PO₄0.3-0.5g/L, CaCl₂·2H₂O.1- 0.3g/L, K₂HPO₄0.3-0.5g/L, pH 6.5-7.5, the same seeding tank of control condition are cultivated 64-96 hours, when thallus gemma shape When at 90-95%, terminates fermentation, obtain the lichen bacillus ferments liquid；After passing it through film concentration, then middle low temperature drying, After being crushed, cell concentration >=10,000,000,000 CFU/g solid powders is spare；

Step 3: the preparation of bacillus laterosporus: the lichem bacillus strain being stored under -4 DEG C of environment is seeded to 30 DEG C of culture 48h, picking single colonie are seeded in beef extract-peptone fluid nutrient medium on beef peptone plate, beef extract egg White peptone culture medium: beef extract 3.0g/L, peptone 10.0g/L, sodium chloride 5.0g/L, pH 7.2-7.6 are 20-30 DEG C in temperature In the environment of cultivate for 24 hours after, obtain required inoculation liquid；Inoculation liquid is inoculated in the 300L seeding tank equipped with seed culture medium, Seed culture medium: peptone 10.0-15.0g/L, glucose 10.0-15.0g/L, sodium chloride 5.0-10.0g/L, beef extract 5.0- 8.0g/L, potassium chloride 1.0-5.0g/L, potassium dihydrogen phosphate 1.0-2.0g/L, magnesium sulfate 0.5-1.0g/L, ferrous sulfate 0.01- In the liquid asepsis culture medium of 0.05g/L, under the conditions of 20-40 DEG C, 150-250r/min shaking table is after 48-72 days, Spawn incubation Stop culture when the OD600 value of liquid is between 2.0-4.0, first order seed is made；Secondary seed culture: liquid asepsis culture medium is pressed Volume ratio be 5-10% inoculum concentration, first order seed is inoculated into fermentor, under the conditions of 20-45 DEG C, mixing speed is 150-250r/min, ventilation ratio is 1:0.6-1.6, after shaking table 72-120 days, secondary seed is made, according to secondary seed culture Same step and method three-level bacterium solution is made, after concentration, cell concentration >=10,000,000,000 CFU/g after middle low temperature drying crushes Solid powder, it is spare；

Step 4: the preparation of saccharomycete: the yeast strain being stored under -4 DEG C of environment being seeded to beef peptone and is put down 30 DEG C of culture 48h, picking single colonie are seeded in beef extract-peptone fluid nutrient medium on plate, beef-protein medium: Beef extract 8.0g/L, peptone 20.0g/L, sodium chloride 5.0g/L, pH 7.2-7.6 are trained in the environment of temperature is 20-35 DEG C After supporting for 24 hours, required inoculation liquid is obtained；Inoculation liquid is inoculated in the 300L seeding tank equipped with seed culture medium, seed culture medium: Glucose 5.0-10.0g/L, peptone 8.0-10.0g/L, yeast extract 3.0-5.0g/L, KCl 0.5-1.0g/L, MgSO₄· 7H₂O 0.8-1.0g/L, FeSO₄0.03-0.05g/L, connecing bacterium amount is 5-10%, is fermented after connecing bacterium, the initial pH value of fermentation 6.5-7.5,20-40 DEG C of temperature, revolving speed 150-250r/min, ventilation ratio are 1:0.6-1.6, when seeding tank fermentation 30-50 is small When, thalli growth is in logarithmic phase, and the fermentation liquid in seeding tank is added to the 3000L fermentor equipped with fermentation medium at this time Supervention ferment is relayed, fermentation medium: mung bean sprouts 50.0-80.0g/L (is cooked into bean sprout juice), potato starch 20.0-30.0g/L, sugarcane Sugared 8.0-12.0g/L, yeast extract 3.0-5.0g/L, KCl 0.5-1.0g/L, MgSO₄·7H₂O 0.3-0.5g/L, NaH₂PO₄0.3-0.5g/L, CaCl₂·2H₂O0.1-0.3g/L, pH 6.5-7.8, the same seeding tank of control condition cultivate 64-96 Hour, as thallus sporulation 90-95%, terminates fermentation, obtain saccharomycetes to make fermentation liquid；After passing it through film concentration, then Middle low temperature drying, after being crushed, cell concentration >=10,000,000,000 CFU/g solid powders is spare；

Step 5: the preparation of colloid bacillus cereus: extremely by the colloid bacillus cereus strain inoculated being stored under -4 DEG C of environment 30 DEG C of culture 48h, picking single colonie are seeded in beef extract-peptone fluid nutrient medium on beef peptone plate, beef extract egg White peptone culture medium: beef extract 3.0g/L, peptone 10.0g/L, sodium chloride 5.0g/L, pH 7.2-7.6 are 18-35 DEG C in temperature In the environment of cultivate for 24 hours after, obtain required inoculation liquid；Inoculation liquid is inoculated in the 300L seeding tank equipped with seed culture medium, Seed culture medium: glucose 8.0-10.0g/L, peptone 8.0-15.0g/L, yeast extract 3.0-5.0g/L, sucrose 3.0-5.0g/ L, beef extract 2.0-5.0g/L, NaH₂PO₄0.2-0.5g/L, MgSO₄·7H₂O 0.2-0.5g/L, connecing bacterium amount is 5-10%, is connect It ferments after bacterium, the initial pH value 6.8-7.5 of fermentation, 20-40 DEG C of temperature, revolving speed 150-250r/min, ventilation ratio is 1: 0.6-1.6, when seeding tank fermentation 30-50 is small, thalli growth is in logarithmic phase, is at this time added to the fermentation liquid in seeding tank 3000L fermentor equipped with fermentation medium relays supervention ferment, fermentation medium: corn pulp 20.0-30.0g/L, beancake powder 20.0-30.0g/L yeast powder 3.0-5.0g/L, MgSO₄·7H₂O0.3-0.5g/L, NaH₂PO₄0.2-0.5g/L, CaCl₂· 2H₂O.2-0.5g/L, K₂HPO₄0.3-0.5g/L, pH 6.5-7.8, the same seeding tank of control condition are cultivated 64-96 hours, work as bacterium When body sporulation 90-95%, terminates fermentation, obtain colloid bacillus cereus fermentation liquid；Pass it through film concentration after, then in it is low Temperature is dry, and after being crushed, cell concentration >=5,000,000,000 CFU/g solid powders is spare；

Step 6: microbial bacterial agent is prepared: by the bacillus subtilis of above-mentioned preparation, bacillus licheniformis, side spore gemma Bacillus, saccharomycete, colloid bacillus cereus solid thalli powder are mixed according to the weight ratio of design, spare；

Step 7: the preparation of microorganism adsorption material: 0.2-5 parts of potassium fulvates, 1-2.5 parts of humic acids, 0.2-2 parts of amino acid Microelement in fertilizer, 0.01-0.1 parts, 0.2-2 parts of modified biomass charcoals, 0.05-0.1 parts of peptones according to design weight Share is spare than being uniformly mixed；

Step 8: the absorption and embedding of microorganism: concentrated biological fermentation liquid is first mixed with prepared microbial bacteria It is even, then it is uniformly mixed again with spare microorganism adsorption material, it is spare.Since humic acid and modified biomass charcoal play microorganism To the effect of absorption and embedding, contact of the microorganism with chemical raw material is reduced, the activity and extension function of functional microorganism are kept The microorganism shelf-life；

Step 9: the embedding method of chemical fertilizer raw material: by remaining potassium fulvate, humic acid, amino acid fertilizer, modification biological Matter charcoal, peptone and chemical fertilizer raw material: potassium nitrate, urea, potassium sulfate, complete water-soluble ammonium phosphate, potassium hydrogen phosphate, middle microelement according to The weight quota of design is spare than being uniformly mixed.Since to be easy to moisture absorption sticky for potassium fulvate, amino acid fertilizer and peptone Most chemical raw material can be embedded, play the buffer action with microorganism by feature, further be conducive to keep function The activity of energy microorganism and extension function microorganism shelf-life；

Step 10: the preparation of the high organic complex microorganism punching property applied fertilizer: by step 8 absorption and embedded micro- life Object microbial inoculum mixture is uniformly mixed with by the embedded chemical fertilizer raw material etc. of step 9, can be prepared by high organic composite microbial Object rushes the property applied fertilizer.

After adopting the above technical scheme, the invention has the following beneficial effects: being to fight solanaceous crops root rot and tune with preferred pin Section soil physico-chemical property bacillus subtilis, bacillus licheniformis, bacillus laterosporus, saccharomycete and colloid bacillus cereus be Strain, by interworking after the independent Fermented Condensed of strain, with the embedding such as potassium fulvate, amino acid fertilizer chemical fertilizer raw material potassium nitrate, After urea, potassium sulfate, complete water-soluble ammonium phosphate, potassium hydrogen phosphate, middle microelement, mixed with absorption and embedded microbial bacterial agent It uniformly forms, compared with existing punching fertilising or composite microbe fertilizer, has the advantage that (1) fertilising of rushing of the invention contains Machine matter, inorganic fertilizer and beneficial microbe function bacterium, making up compound organic and inorganic fertilizer does not have the shortcomings that functional microorganism, composite microbial The low disadvantage of the object fertilizer content of organic matter；(2) of the invention is the solanaceous crops developed according to the growth characteristics of solanaceous crops Spraying fertilizer is rushed, the most of raw material used has water solubility, and the particle of insoluble substance is tiny also at suspension, does not block punching Spray head is applied, can satisfy the requirement of punching fertilising during solanaceous crops facility cultivation；(3) preferred pin to solanaceous crops root rot and Adjust bacillus subtilis, bacillus licheniformis, bacillus laterosporus, saccharomycete and the colloid gemma bar of soil physico-chemical property Bacterium, not simple proportion combination, synergistic effect, while the physicochemical property of synergic adjustment soil, form beneficial rhizosphere Micro-ecological environment enhances the disease resistance of solanaceous crops, nutrition-concerted, and water conservation, fertilizer conservation can prevent solanaceous crops root-rot disease Occur；(4) pass through the absorption of microbial bacterial agent and the mixing progress of other raw materials and be embedded in modified biomass charcoal and rotten plant Acid is to play the role of protecting microorganism, while it is sticky using potassium fulvate and amino acid fertilizer to be easy to moisture absorption in carrier It is first mixed with chemical raw material and plays embedding effect and humic acid and modified biomass charcoal is micro- to function by feature Biology plays suction-operated, makes being isolated for functional microorganism and chemical fertilizer, is further conducive to the activity for keeping functional microorganism and prolongs The long functional microorganism shelf-life；(5) substances such as potassium fulvate, humic acid, modified biomass charcoal, amino acid fertilizer, with function Microorganism is applied together to soil, is conducive to functional microorganism and is preferably colonized in the soil；(6) humic acid, amino acid fertilizer, Fulvic acid contains some active groups and functional microorganism microbial inoculum and is generating the biological active matters such as some indole acids, gibberellic acid Matter can stimulate crop growth, improve enzyme activity, the disease-resistant degeneration-resistant effect of enhancing, to take root, growth promoting, flower and fruit protecting have one Fixed effect is conducive to the high and stable yields of solanaceous crops；

Solanaceous crops high organic complex microorganism flushing and spreading fertilizer of the invention increases production, upgrading effect is very significant.2017- In the greenhouse facility cultivated peppers and eggplant field experiment in village, salon, Liuyang City, solanaceous crops height of the invention is used within 2018 Organic matter complex microorganism flushing and spreading fertilizer and local habits fertilization scheme (entirely to fertilize) and common complex microorganism punching fertilising phase Than, solanaceous crops solanaceous crops high organic complex microorganism flushing and spreading fertilizer of the invention have stem thickness, Shu Wang, volume increase, loam, Root rot disease is few.In the cultivation of capsicum greenhouse facility, processing is applied compared to chemical fertilizer punching, yield can be improved 17.8%, compared to general Processing is applied in logical complex microorganism punching, and yield can be improved 8.5%.In the cultivation of eggplant greenhouse facility, place is applied compared to chemical fertilizer punching Reason, yield can be improved 23.4%, apply processing compared to common complex microorganism punching, yield can be improved 13.3%.Explanation is applied With solanaceous crops high organic complex microorganism flushing and spreading fertilizer of the invention, it is possible to reduce solanaceous crops greenhouse facility cultivation In root rot disease occur, increase yield, improve the effect of quality of agricultural product.

Specific embodiment

Embodiment 1

Present embodiment the technical solution adopted is that: a kind of punching fertilising of solanaceous crops high organic complex microorganism Material, it is made of the raw material of following parts by weight: 65 parts of potassium fulvate, 5 parts of potassium nitrate, 3 parts of urea, 3 parts of potassium sulfate, phosphoric acid 1 part of monoammonium, 1 part of dipotassium hydrogen phosphate, 5 parts of humic acid, 10 parts of amino acid fertilizer, 0.5 part of peptone, concentrated biological fermentation liquid 0.5 Part, 2 parts of modified biomass charcoal, 4 parts of microbial bacterial agent, 1 part of middle microelement；The middle microelement is chelating amino acids Calcium, magnesium sulfate, zinc sulfate, borax, amino acid chelated iron, cerium nitric acid rare earth are according to 5 parts: 5 parts: 5 parts: 5 parts: 1 part of mass ratio: 0.1 part is mixed；The microbial bacterial agent is bacillus subtilis, bacillus licheniformis, bacillus laterosporus, saccharomycete Uniformly mixed, the registration number of bacillus subtilis is carried out than 5 parts: 5 parts: 5 parts: 1 parts: 1 part according to bacterium number with colloid bacillus cereus For CICC 10275, bacillus licheniformis registration number is ATCC11946, bacillus laterosporus ATCC10249, candida utili Bacterium ATCC22023, colloid bacillus cereus registration number are GIM1.16, effective bacterial content >=10,000,000,000 CFU/g of the microbial bacterial agent； The complete water-soluble ammonium phosphate is monoammonium phosphate；The potassium hydrogen phosphate is potassium dihydrogen phosphate.

Step 1: the preparation of bacillus subtilis: the bacillus subtilis strain being stored under -4 DEG C of environment is seeded to 30 DEG C of culture 48h, picking single colonie are seeded in beef extract-peptone fluid nutrient medium on beef peptone plate, beef extract egg White peptone culture medium: beef extract 3.0g/L, peptone 10.0g/L, sodium chloride 5.0g/L, pH 7.2-7.6 are 20-35 DEG C in temperature In the environment of cultivate for 24 hours after, obtain required inoculation liquid；Inoculation liquid is inoculated in the 300L seeding tank equipped with seed culture medium, Seed culture medium: peptone 10.0g/L, yeast extract 3.0g/L, beef extract 5.0g/L, glucose 5.0g/L, sodium chloride 3.0g/L, MgSO₄·7H₂O 0.5g/L, K₂HPO₄0.5g/L, FeCl₃0.05g/L, connecing bacterium amount is 5-10%, is fermented after connecing bacterium, is fermented Initial pH value 6.8-7.5,28-35 DEG C of temperature, revolving speed 150-250r/min, ventilation ratio be 1:0.6-1.6, when seeding tank send out Ferment 30-50 hours, thalli growth was in logarithmic phase, and the fermentation liquid in seeding tank is added to equipped with fermentation medium at this time 3000L fermentor relays supervention ferment, fermentation medium: corn pulp 30.0g/L, beancake powder 50.0g/L, yeast powder 5.0g/L, sugarcane Sugared 8.0g/L, MgSO₄·7H₂O 0.5g/L, NaH₂PO₄1.0g/L, CaCl₂·2H₂O 0.5g/L, K₂HPO₄0.5g/L, FeCl₃0.05g/L, pH 6.5-7.8, the same seeding tank of control condition are cultivated 64-96 hours, as thallus sporulation 90-95% When, terminate fermentation, obtains bacillus subtilis fermentation liquor；After passing it through film concentration, then middle low temperature drying, is crushed Afterwards, cell concentration >=10,000,000,000 CFU/g solid powders, it is spare；

Step 2: the preparation of bacillus licheniformis: the lichem bacillus strain being stored under -4 DEG C of environment is seeded to 30 DEG C of culture 48h, picking single colonie are seeded in beef extract-peptone fluid nutrient medium on beef peptone plate, beef extract egg White peptone culture medium: beef extract 3.0g/L, peptone 10.0g/L, sodium chloride 5.0g/L, pH 7.2-7.6 are 20-30 DEG C in temperature In the environment of cultivate for 24 hours after, obtain required inoculation liquid；Inoculation liquid is inoculated in the 300L seeding tank equipped with seed culture medium, Seed culture medium: glucose 10.0g/L, peptone 15.0g/L, yeast extract 5.0g/L, beef extract 5.0g/L, MgSO₄·7H₂O 0.5g/L,K₂HPO₄0.3g/L, connecing bacterium amount is 5-10%, is fermented after connecing bacterium, the initial pH value 6.5-7.5 of fermentation, temperature 20-45 DEG C, revolving speed 150-250r/min, ventilation ratio are 1:0.6-1.6, when seeding tank fermentation 30-50 is small, at thalli growth In logarithmic phase, the fermentation liquid in seeding tank is added to the relaying supervention ferment of the 3000L fermentor equipped with fermentation medium, hair at this time Ferment culture medium: corn pulp 30.0g/L, beancake powder 30.0g/L, yeast powder 5.0g/L, sucrose 5.0g/L, MgSO₄·7H₂O 0.5g/ L, NaH₂PO₄0.5g/L, CaCl₂·2H₂0.3g/L, K₂HPO₄0.5g/L, pH 6.5-7.5, the same seeding tank of control condition, culture 64-96 hours, as thallus sporulation 90-95%, terminates fermentation, obtain the lichen bacillus ferments liquid；Pass it through film After concentration, then middle low temperature drying, after being crushed, cell concentration >=10,000,000,000 CFU/g solid powders is spare；

Step 3: the preparation of bacillus laterosporus: the lichem bacillus strain being stored under -4 DEG C of environment is seeded to 30 DEG C of culture 48h, picking single colonie are seeded in beef extract-peptone fluid nutrient medium on beef peptone plate, beef extract egg White peptone culture medium: beef extract 3.0g/L, peptone 10.0g/L, sodium chloride 5.0g/L, pH 7.2-7.6 are 20-30 DEG C in temperature In the environment of cultivate for 24 hours after, obtain required inoculation liquid；Inoculation liquid is inoculated in the 300L seeding tank equipped with seed culture medium, Seed culture medium: peptone 10.0g/L, glucose 10.0g/L, sodium chloride 5.0g/L, beef extract 5.0g/L, potassium chloride 1.0g/ L, in the liquid asepsis culture medium of potassium dihydrogen phosphate 1.0g/L, magnesium sulfate 0.5g/L, ferrous sulfate 0.01g/L, in 20-40 DEG C of item Under part, 150-250r/min shaking table stopped culture, system after 48-72 days when the OD600 value of bacteria culture fluid is between 2.0-4.0 Obtain first order seed；Secondary seed culture: the inoculum concentration for being 5-10% by the volume ratio of liquid asepsis culture medium connects first order seed Kind is into fermentor, and under the conditions of 20-45 DEG C, mixing speed 150-250r/min, ventilation ratio is 1:0.6-1.6, shaking table After 72-120 days, secondary seed is made, three-level bacterium solution is made according to the same step and method of secondary seed culture, by concentration Afterwards, cell concentration >=10,000,000,000 CFU/g solid powders after middle low temperature drying crushes, it is spare；

Step 4: the preparation of saccharomycete: the yeast strain being stored under -4 DEG C of environment being seeded to beef peptone and is put down 30 DEG C of culture 48h, picking single colonie are seeded in beef extract-peptone fluid nutrient medium on plate, beef-protein medium: Beef extract 8.0g/L, peptone 20.0g/L, sodium chloride 5.0g/L, pH 7.2-7.6 are trained in the environment of temperature is 20-35 DEG C After supporting for 24 hours, required inoculation liquid is obtained；Inoculation liquid is inoculated in the 300L seeding tank equipped with seed culture medium, seed culture medium: Glucose 5.0g/L, peptone 8.0g/L, yeast extract 3.0g/L, KCl 0.5g/L, MgSO₄·7H₂O 0.8g/L, FeSO₄0.03g/L, connect bacterium amount be 5-10%, ferment after connecing bacterium, the initial pH value 6.5-7.5 of fermentation, 20-40 DEG C of temperature, Revolving speed 150-250r/min, ventilation ratio are 1:0.6-1.6, and when seeding tank fermentation 30-50 is small, thalli growth is in logarithm Fermentation liquid in seeding tank is added to the relaying supervention ferment of the 3000L fermentor equipped with fermentation medium, fermented and cultured at this time by the phase Base: mung bean sprouts 50.0g/L (is cooked into bean sprout juice), potato starch 20.0g/L, sucrose 8.0g/L, yeast extract 3.0g/L, KCl 0.5g/L, MgSO₄·7H₂O 0.3g/L, NaH₂PO₄0.3g/L, CaCl₂·2H₂O 0.1g/L, pH 6.5-7.8, control condition Same seeding tank is cultivated 64-96 hours, as thallus sporulation 90-95%, is terminated fermentation, is obtained saccharomycetes to make fermentation liquid；By its After membrane concentration, then middle low temperature drying, after being crushed, cell concentration >=10,000,000,000 CFU/g solid powders is spare；

Step 5: the preparation of colloid bacillus cereus: extremely by the colloid bacillus cereus strain inoculated being stored under -4 DEG C of environment 30 DEG C of culture 48h, picking single colonie are seeded in beef extract-peptone fluid nutrient medium on beef peptone plate, beef extract egg White peptone culture medium: beef extract 3.0g/L, peptone 10.0g/L, sodium chloride 5.0g/L, pH 7.2-7.6 are 18-35 DEG C in temperature In the environment of cultivate for 24 hours after, obtain required inoculation liquid；Inoculation liquid is inoculated in the 300L seeding tank equipped with seed culture medium, Seed culture medium: glucose 8.0g/L, peptone 8.0g/L, yeast extract 3.0g/L, sucrose 5.0g/L, beef extract 2.0g/L, NaH₂PO₄0.2g/L, MgSO₄·7H₂O 0.2g/L, connecing bacterium amount is 5-10%, is fermented after connecing bacterium, the initial pH value of fermentation 6.8-7.5,20-40 DEG C of temperature, revolving speed 150-250r/min, ventilation ratio are 1:0.6-1.6, when seeding tank fermentation 30-50 is small When, thalli growth is in logarithmic phase, and the fermentation liquid in seeding tank is added to the 3000L fermentor equipped with fermentation medium at this time Relay supervention ferment, fermentation medium: corn pulp 30.0g/L, beancake powder 30.0g/L, yeast powder 5.0g/L, MgSO₄·7H₂O 0.5g/L, NaH₂PO₄0.2g/L, CaCl₂·2H₂O.2g/L, K₂HPO₄0.3g/L, pH 6.5-7.8, the same seeding tank of control condition, Culture 64-96 hours terminates fermentation, obtains colloid bacillus cereus fermentation liquid as thallus sporulation 90-95%；It is passed through After crossing film concentration, then middle low temperature drying, after being crushed, cell concentration >=5,000,000,000 CFU/g solid powders is spare；

Step 7: microbial bacterial agent absorption material preparation: 1 part of potassium fulvate, 5 parts of humic acids, 1 part of amino acid fertilizer, Microelement, 2 parts of modified biomass charcoals, 0.05 part of peptone are uniformly mixed according to the weight quota ratio of design in 0.01 part, standby With；

Step 8: the absorption and embedding of microbial bacterial agent: by concentrated biological fermentation liquid first with prepare microbial bacterial agent and mix It closes uniformly, is then uniformly mixed with spare microbial bacterial agent absorption material, it is spare.Due to humic acid and modified biomass charcoal pair Microorganism plays the role of absorption and embedding, reduces contact of the microorganism with chemical raw material, keep functional microorganism activity and The extension function microorganism shelf-life；

Step 9: the embedding method of chemical fertilizer raw material: remaining potassium fulvate, amino acid fertilizer, peptone and chemical fertilizer is former Material: potassium nitrate, urea, potassium sulfate, complete water-soluble ammonium phosphate, potassium hydrogen phosphate, middle microelement are according to the weight quota of design than mixed It closes uniformly, it is spare.It, can be by major part since potassium fulvate, amino acid fertilizer and peptone are easy to the sticky feature of moisture absorption Chemical raw material embedded, play the buffer action with microorganism, be further conducive to keep functional microorganism activity and The extension function microorganism shelf-life；

Complex microorganism punching fertilising obtained according to the method described above, meets following index:

(1) organic matter >=45%；

(2)N+P₂O₅+K₂O >=15%；

(3) moisture content≤30%；

(4) miscellaneous bacteria number≤15%；

(5) amino acid >=8%

(6) effectively bacterium number >=400,000,000 CFU/g；

(7) validity period is >=12 months.

Points for attention: the high organic complex microorganism punching property the applied fertilizer of the present embodiment does chasing after for solanaceous crops facility cultivation Fertilizer punching application, suitably keeps ground moistening after application, the high organic complex microorganism punching property applied of the present embodiment can be improved The function and fertilizer efficiency of fertilizer.Due to containing microbial flora in special fertilizer, cannot mix make with fungicide or antibacterial material With.Pay attention to avoiding strong illumination when preservation, prevent with strong acid and strong base stack etc..

The solanaceous crops high organic content complex microorganism flushing and spreading fertilizer of the production of the present embodiment is as capsicum top dressing Experimental program and application effect:

1, experimental design

The planting base greenhouse cultivation (first year cultivation is capsicum) tested in salon, Liuyang City carries out, and experimental cultivar is capsicum Fine work nine, spacing in the rows 45cm, line-spacing 50cm.Test sets 3 processing: processing 1: (potassium sulfate type of 15-15-15 is multiple to fertilize entirely Hefei) 120kg/ mus；Processing 2: it applies the punching fertilising of commodity complex microorganism and does top dressing application, 22% nutrient formula is 11-5- 6, dosage is 95.45kg/ mus；Processing 3: it is water-soluble to apply solanaceous crops high organic content described in embodiment 1 in the present invention Top dressing application is done in complex microorganism punching fertilising, and 22% nutrient formula is 12-4-6, and dosage is 87.5kg/ mus.Fertilizer applications bottom The base fertilizer of fertilizer, top dressing, three processing all uses the potassium sulfate compound fertilizer cave of 15-15-15 to apply, and sowing amount is 50 kgs/acre, chases after Fertilizer is all that rushing for water-fertilizer integral is applied, point 10 progress, top dressing, about 15-25d or so returning after seedling after capsicum transplanting, Shi Yici is just rushed after harvesting a collection of pepper fruit, chance, which is rained, or soil is very wet postpones fertilising.Each processing is applied every time Fertilizer amount (including base fertilizer and 10 top dressings) is all identical, and commodity complex microorganism punching fertilising and high organic described in embodiment 1 contain When measuring the punching each top dressing of fertilising of Water Soluble Compound microorganism, the insufficient change with good water solubilities such as potassium hydrogen phosphate and potassium sulfates of nutrient Fertilizer is supplied, that is, guarantees N=1.05kg/ mus, P when each top dressing punching fertilising₂O₅=1.05kg/ mus, K₂O=1.05kg/ mus, in this way Ensure that each entire breeding time dose of processing all in accordance with N=18kg/ mus complete, P₂O₅=18kg/ mus, K₂O=18kg/ mus etc. Nutrition calculation.Every processing is repeated 3 times, and amounts to 9 cells, and every plot area is 15m².Capsicum in detail after each harvesting of record Yield, take first fruiting period measure each cell capsicum plant height (length of basal part of stem to growing point), tree width, stem thickness (basal part of stem it is thick Degree) and newly foliation number plays the 5th SPAD value.It produces fruit later period collection rhizosphere surrounding soil and measures bacterium, fungi, actinomyces, together When measurement rhizosphere around be volume 1m × 1m × 0.5m earthworm quantity, 3 points of each cell sampling, every investigation be connected 7 plants, with Strain is that unit is investigated according to the classification of wilt disease occurring degree difference, record investigation total strain number, diseased plant number at different levels.Stage division:

0 grade: complete stool is disease-free；

1 grade: the slight browning of basal part of stem, chili growth are normal；

3 grades: the obvious browning of basal part of stem, it is with softening and slightly rotten；

5 grades: the obvious browning of basal part of stem and rot, lobus cardiacus wither hang down crispatura；

7 grades: basal part of stem browning rots, and the withered or yellowish-brown of complete stool blueness is withered.

Diseased plant rate (%)=diseased plant number/investigation total strain number × 100%；

Disease index (%)=∑ (diseased plant numbers at different levels × opposite value of series)/(investigation total strain number × 7) × 100%；

Opposite control efficiency (%)=(control disease incidence-processing disease incidence)/control disease incidence × 100%.

Other not specified index determinings are carried out with reference to the methods of related national standard.

2, main result

Influence of the 1 different type fertilizer of table to continuous cropping chili growth and yield index of correlation

Note: institute's column data is 3 duplicate average values in table, and different letters indicate that difference reaches significant water after same column numerical value It is flat, similarly hereinafter.

As can be seen from Table 1, the continuous cropping capsicum tree that embodiment 1 is handled is high, it is all aobvious to set stem thickness, tree width and the fresh yield of per hectare It writes and is higher than chemical fertilizer processing, the fresh yield of per hectare has increased production 17.84% than chemical fertilizer processing, rushes fertilizer treatment than complex microorganism and increases Produce 8.49%.Illustrate embodiment 1 under fertilizing nutrient and all identical situation of fertilizer applications, effect of increasing production is obvious.

Influence of the 2 different type fertilizer of table to continuous cropping capsicum rhizosphere microorganism and earthworm quantity

As can be seen from Table 3, the quantity of embodiment 1 is handled continuous cropping capsicum soil bacteria, actinomyces, fungi and earthworm is all It is significantly higher than chemical fertilizer and composite microbe fertilizer rushes fertilizer treatment.Although the quantity of 1 fungi of embodiment is significant to be apparently higher than its elsewhere Reason, but its soil types is still based on the micro organism quantity of bacteria type, mainly increasing due to the soil organism, it is native Earth micro organism quantity all significantly increases.Therefore, embodiment 1 handles micro organism quantity and dramatically increases the fertilizer that soil can be improved Significantly increasing for power, especially earthworm quantity, can improve soil texture, reduce soil hardening, improve soil porosity, excellent Change soil quality, enhances soil permeability.

Influence of the 3 different type fertilizer of table to continuous cropping capsicum wilt disease index

As can be seen from Table 3, the high organic content complex microorganism punching for using embodiment 1 to prepare is applied fertilizer as continuous cropping capsicum Top dressing punching is applied, and the disease incidence and disease index of continuous cropping capsicum are substantially lower than chemical fertilizer and commodity composite microbe fertilizer, opposite The opposite control efficiency of chemical fertilizer and commodity composite microbe fertilizer is respectively 74.29% and 48.87%.Therefore, it applies of the invention The punching fertilising of high organic content complex microorganism, can promote the growth of capsicum, reduce the generation of continuous cropping capsicum wilt, more suitable The water-fertilizer integral facility cultivation of high fertile Gao Shui is closed, synergy of earthing up.

Embodiment 2

A kind of solanaceous crops high organic complex microorganism flushing and spreading fertilizer, it is made of the raw material of following parts by weight: 60 parts of potassium fulvate, 15 parts of potassium nitrate, 2 parts of urea, 4 parts of potassium sulfate, 5 parts of Diammonium phosphate (DAP), 2 parts of potassium dihydrogen phosphate, humic acid 2 Part, 5 parts of amino acid fertilizer, 0.5 part of peptone, 0.5 part of concentrated biological fermentation liquid, 2 parts of modified biomass charcoal, microbial bacterial agent 1 Part, 1 part of middle microelement；The middle microelement is amino acid chelated calcium, magnesium sulfate, zinc sulfate, borax, chelating amino acids Iron, cerium nitric acid rare earth are mixed according to mass ratio 2:2:2:2:1:0.05；The microbial bacterial agent be bacillus subtilis, Bacillus licheniformis, bacillus laterosporus, saccharomycete, colloid bacillus cereus carried out according to bacterium number ratio 2:2:2:1:1 it is uniformly mixed, The registration number of bacillus subtilis is CICC10275, and bacillus licheniformis registration number is ATCC11946, bacillus laterosporus ATCC10249, candida utili bacterium ATCC22023, colloid bacillus cereus registration number are GIM1.16, the microbial bacterial agent Effective bacterial content >=10,000,000,000 CFU/g.The complete water-soluble ammonium phosphate is Diammonium phosphate (DAP)；The potassium hydrogen phosphate is dipotassium hydrogen phosphate.

The preparation method and step of remaining are identical with embodiment 1.

(1) organic matter >=40%；

(2)N+P₂O₅+K₂O >=22%；

(3) moisture content≤30%；

(4) miscellaneous bacteria number≤15%；

(5) amino acid >=5%

(6) effectively bacterium number >=100,000,000 CFU/g；

(7) validity period is >=12 months.

Points for attention are the same as embodiment 1.

Reality of the solanaceous crops high organic content complex microorganism flushing and spreading fertilizer of the present embodiment production as eggplant top dressing Proved recipe case and application effect:

1, experimental design

It tests the planting base greenhouse cultivation in village, salon, Liuyang City and carries out (cultivation in head 2 years is all eggplant), experimental cultivar It is eggplant spring eggplant ten No. six, spacing in the rows 50cm, line-spacing 60cm.Test sets 3 processing: processing 1: the complete (sulphur of 20-10-20 to fertilize Suan Jia type compound fertilizer) 120kg/ mus；Processing 2: it applies the punching fertilising of commodity complex microorganism and does top dressing application, 22% nutrient formula For 11-5-6, dosage is 127.27kg/ mus；Processing 3: it applies solanaceous crops high organic described in embodiment 1 in the present invention and contains Amount Water Soluble Compound microorganism flushing and spreading fertilizer does top dressing application, and 22% nutrient formula is 12-4-6, dosage 116.67kg/ Mu.The base fertilizer of fertilizer applications base fertilizer, top dressing, three processing all uses the potassium sulfate compound fertilizer cave of 20-10-20 to apply, sowing amount It is 50 kgs/acre, top dressing is all that rushing for water-fertilizer integral is applied, point 10 progress, top dressing returning after seedling after eggplant transplanting, about For 15-25d or so, Shi Yici is just rushed after harvesting a collection of eggplant fruit, chance, which is rained, or soil is very wet postpones fertilising.Each Each dose (including base fertilizer and 10 top dressings) of processing is all identical, described in commodity complex microorganism punching fertilising and embodiment 2 The punching of high organic content Water Soluble Compound microorganism apply fertilizer each top dressing when, nutrient is insufficient with potassium hydrogen phosphate and potassium sulfate etc. The chemical fertilizer of good water solubility is supplied, that is, guarantees N=1.2kg/ mus, P when each top dressing punching fertilising₂O₅=0.6kg/ mus, K₂O= 1.2kg/ mus, ensure that each entire breeding time dose of processing all in accordance with N=24kg/ mus complete, P in this way₂O₅=12kg/ mus, K₂The nutrition calculations such as O=24kg/ mus.Every processing is repeated 3 times, and amounts to 9 cells, and every plot area is 20m².Record is each in detail Eggplant yield after harvesting takes first fruiting period to measure the plant height (length of basal part of stem to growing point) of each cell eggplant, tree width, stem thickness (rugosity of basal part of stem) and new foliation number play the 5th SPAD value.Producing the fruit later period collects rhizosphere surrounding soil measurement bacterium, true Bacterium, actinomyces, while measuring around rhizosphere as volume 1m × 1m × 0.5m earthworm quantity, 3 points of each cell samplings, every tune Connected 7 plants are looked into, is classified and is investigated according to wilt disease occurring degree difference as unit of strain, record investigation total strain number, diseased plant number at different levels. Stage division:

0 grade: complete stool is disease-free；

1 grade: the slight browning of basal part of stem, eggplant growth are normal；

2, main result

1 different type fertilizer of table is grown to eggplant and the influence of yield index of correlation

As can be seen from Table 1, the eggplant tree that embodiment 2 is handled is high, it is all significantly high to set stem thickness, tree width and the fresh yield of per hectare In chemical fertilizer processing, the fresh yield of per hectare has increased production 23.41% than chemical fertilizer processing, has rushed fertilizer treatment than complex microorganism and increase production 13.33%.Illustrate embodiment 2 under fertilizing nutrient and all identical situation of fertilizer applications, the effect of increasing production of eggplant is obvious.

Influence of the 2 different type fertilizer of table to eggplant rhizosphere microorganism and earthworm quantity

As can be seen from Table 2, the quantity of embodiment 2 is handled eggplant soil bacteria, actinomyces, fungi and earthworm is all significant Fertilizer treatment is rushed higher than chemical fertilizer and composite microbe fertilizer.Although the quantity of 2 fungi of embodiment is significant to be apparently higher than other processing, It is its soil types or based on the micro organism quantity of bacteria type, mainly increasing due to the soil organism, soil is micro- Biomass all significantly increases.Therefore, embodiment 2 handles micro organism quantity and dramatically increases the fertility that soil can be improved, Especially the significant of earthworm quantity increases, and can improve soil texture, reduces soil hardening, improves soil porosity, optimization Soil quality enhances soil permeability.

Influence of the 3 different type fertilizer of table to wilt of eggplant disease index

As can be seen from Table 3, the high organic content complex microorganism punching for using embodiment 2 to prepare is applied fertilizer as eggplant top dressing Punching is applied, and the disease incidence and disease index of eggplant are substantially lower than chemical fertilizer and commodity composite microbe fertilizer, opposite chemical fertilizer and quotient The opposite control efficiency of product composite microbe fertilizer is respectively 78.57% and 61.29%.Therefore, high organic of the invention is applied The punching fertilising of content complex microorganism, can promote the growth of eggplant, reduce the generation of wilt of eggplant, be more suitable for high fertile Gao Shui's Water-fertilizer integral facility cultivation, synergy of earthing up.The above is only used to illustrate the technical scheme of the present invention and not to limit it, ability The other modifications or equivalent replacement that domain those of ordinary skill makes technical solution of the present invention, without departing from skill of the present invention The spirit and scope of art scheme, are intended to be within the scope of the claims of the invention.

Claims

1. a kind of high organic complex microorganism flushing and spreading fertilizer, which is characterized in that it is made of the raw material of following parts by weight: 30-85 parts of potassium fulvate, 5-25 parts of potassium nitrate, 1-10 parts of urea, 1-10 parts of potassium sulfate, complete water-soluble ammonium phosphate 1-10 parts, phosphoric acid 1-5 parts of hydrogen potassium, 1-5 parts of humic acid, 0.2-2 parts of peptone, 0.2-2 parts of concentrated biological fermentation liquid, changes 4-10 parts of amino acid fertilizer It is 0.2-4 parts of biomass carbon of property, 0.2-5 parts of microbial bacterial agent, microelement 0.2-2 parts middle；

The potassium fulvate and humic acid is biochemical treated water-soluble substances；The amino acid is the animal sources of commodity Amino acid is using various ocean fish processing factories leftover bits and pieces, boiling pulp-water as raw material, through microbial fermentation solution and biodegradable albumen Matter, after generating some new active materials, spray drying is process；The concentrated biological fermentation liquid is that the liquid of commodity is raw Object fermentation liquid is to be generated by organic goods, materials and equipments such as cane molasses, bagasse by deep-layer liquid biofermentation technique；Described changes Property biomass carbon be husk after the carbonized substance that anaerobism burn incompletely is formed, then through ball mill ball milling at nanoscale Grain, using the material of the sieve of 500 mesh；The middle microelement be amino acid chelated calcium, magnesium sulfate, zinc sulfate, borax, Amino acid chelated iron, cerium nitric acid rare earth are according to 1~10 part: 1~10 part: 1~5 part: 1~5 part: 0~1 part: 0~0.1 of mass ratio Part is mixed；The microbial bacterial agent is bacillus subtilis, bacillus licheniformis, bacillus laterosporus, saccharomycete, glue Matter bacillus is according to bacterium number than 1~10 part: 1~10 part: 1~10 part: 0~5 part: 0~5 part is mixed, microbial bacterial agent Effective bacterial content >=10,000,000,000 CFU/g；The complete water-soluble ammonium phosphate is monoammonium phosphate or Diammonium phosphate (DAP)；The potassium hydrogen phosphate is Potassium dihydrogen phosphate or dipotassium hydrogen phosphate.

2. a kind of preparation method of high organic complex microorganism flushing and spreading fertilizer, which is characterized in that it is comprised the steps of:

Step 1: the preparation of bacillus subtilis: the bacillus subtilis strain being stored under -4 DEG C of environment is seeded to beef 30 DEG C of culture 48h, picking single colonie are seeded in beef extract-peptone fluid nutrient medium on peptone plate, beef extract-peptone Culture medium: beef extract 3.0g/L, peptone 10.0g/L, sodium chloride 5.0g/L, pH 7.2-7.6, the ring for being 20-35 DEG C in temperature After cultivating for 24 hours under border, required inoculation liquid is obtained；Inoculation liquid is inoculated in the 300L seeding tank equipped with seed culture medium, seed Culture medium: peptone 8.0-15.0g/L, yeast extract 2.0-5.0g/L, beef extract 3.0-5.0g/L, glucose 3.0-5.0g/L, Sodium chloride 2.0-5.0 g/L, MgSO₄·7H₂O 0.3-0.5g/L, K₂HPO₄0.3-0.5g/L, FeCl₃0.01-0.05g/L, Connecing bacterium amount is 5-10%, is fermented after connecing bacterium, the initial pH value 6.8-7.5 of fermentation, 28-35 DEG C of temperature, revolving speed 150-250r/ Min, ventilation ratio are 1:0.6-1.6, and when seeding tank fermentation 30-50 is small, thalli growth is in logarithmic phase, at this time by seeding tank In fermentation liquid be added to the 3000L fermentor equipped with fermentation medium relaying supervention ferment, fermentation medium: corn pulp 20.0- 30.0g/L, beancake powder 30.0-50.0g/L, yeast powder 3.0-5.0g/L, sucrose 5.0-8.0g/L, MgSO₄·7H₂O 0.3- 0.5g/L, NaH₂PO₄0.5-1.0g/L, CaCl₂·2H₂O 0.1-0.5g/L, K₂HPO₄0.3-0.5g/L, FeCl₃0.01- 0.05g/L, pH 6.5-7.8, the same seeding tank of control condition are cultivated 64-96 hours, as thallus sporulation 90-95%, knot Beam fermentation, obtains bacillus subtilis fermentation liquor；After passing it through film concentration, then middle low temperature drying, after being crushed, thallus Concentration >=10,000,000,000 CFU/g solid powders, it is spare；

Step 2: the preparation of bacillus licheniformis: the lichem bacillus strain being stored under -4 DEG C of environment is seeded to beef 30 DEG C of culture 48h, picking single colonie are seeded in beef extract-peptone fluid nutrient medium on peptone plate, beef extract-peptone Culture medium: beef extract 3.0g/L, peptone 10.0g/L, sodium chloride 5.0g/L, pH 7.2-7.6, the ring for being 20-30 DEG C in temperature After cultivating for 24 hours under border, required inoculation liquid is obtained；Inoculation liquid is inoculated in the 300L seeding tank equipped with seed culture medium, seed Culture medium: glucose 5.0-10.0g/L, peptone 8.0-15.0g/L, yeast extract 3.0-5.0g/L, beef extract 3.0-5.0g/L, MgSO₄·7H₂O 0.3-0.5g/L,K₂HPO₄0.1-0.3g/L, connecing bacterium amount is 5-10%, is fermented after connecing bacterium, at the beginning of fermentation Beginning pH value 6.5-7.5,20-45 DEG C of temperature, revolving speed 150-250r/min, ventilation ratio are 1:0.6-1.6, when seeding tank ferments 30-50 hours, thalli growth was in logarithmic phase, and the fermentation liquid in seeding tank is added to equipped with fermentation medium at this time 3000L fermentor relays supervention ferment, fermentation medium: corn pulp 15.0-30.0g/L, beancake powder 20.0-30.0g/L, yeast powder 3.0-5.0g/L sucrose 3-5g/L, MgSO₄·7H₂O 0.3-0.5g/L, NaH₂PO₄0.3-0.5g/L, CaCl₂·2H₂O.1- 0.3g/L, K₂HPO₄0.3-0.5g/L, pH 6.5-7.5, the same seeding tank of control condition are cultivated 64-96 hours, when thallus gemma When forming 90-95%, terminates fermentation, obtain the lichen bacillus ferments liquid；After passing it through film concentration, then middle low temperature is dry Dry, after being crushed, cell concentration >=10,000,000,000 CFU/g solid powders is spare；

Step 3: the preparation of bacillus laterosporus: the lichem bacillus strain being stored under -4 DEG C of environment is seeded to beef 30 DEG C of culture 48h, picking single colonie are seeded in beef extract-peptone fluid nutrient medium on peptone plate, beef extract-peptone Culture medium: beef extract 3.0g/L, peptone 10.0g/L, sodium chloride 5.0g/L, pH 7.2-7.6, the ring for being 20-30 DEG C in temperature After cultivating for 24 hours under border, required inoculation liquid is obtained；Inoculation liquid is inoculated in the 300L seeding tank equipped with seed culture medium, seed Culture medium: peptone 10.0-15.0g/L, glucose 10.0-15.0g/L, sodium chloride 5.0-10.0g/L, beef extract 5.0- 8.0g/L, potassium chloride 1.0-5.0g/L, potassium dihydrogen phosphate 1.0-2.0g/L, magnesium sulfate 0.5-1.0g/L, ferrous sulfate 0.01- In the liquid asepsis culture medium of 0.05g/L, under the conditions of 20-40 DEG C, 150-250r/min shaking table is after 48-72 days, Spawn incubation Stop culture when the OD600 value of liquid is between 2.0-4.0, first order seed is made；Secondary seed culture: liquid asepsis culture medium is pressed Volume ratio be 5-10% inoculum concentration, first order seed is inoculated into fermentor, under the conditions of 20-45 DEG C, mixing speed is 150-250r/min, ventilation ratio is 1:0.6-1.6, after shaking table 72-120 days, secondary seed is made, according to secondary seed culture Same step and method three-level bacterium solution is made, after concentration, cell concentration >=10,000,000,000 CFU/g after middle low temperature drying crushes Solid powder, it is spare；

Step 4: the preparation of saccharomycete: the yeast strain being stored under -4 DEG C of environment is seeded on beef peptone plate 30 DEG C of culture 48h, picking single colonie are seeded in beef extract-peptone fluid nutrient medium, beef-protein medium: beef Cream 8.0g/L, peptone 20.0g/L, sodium chloride 5.0g/L, pH 7.2-7.6 are cultivated for 24 hours in the environment of temperature is 20-35 DEG C Afterwards, required inoculation liquid is obtained；Inoculation liquid is inoculated in the 300L seeding tank equipped with seed culture medium, seed culture medium: grape Sugared 5.0-10.0g/L, peptone 8.0-10.0g/L, yeast extract 3.0-5.0g/L, KCl 0.5-1.0g/L, MgSO₄·7H₂O 0.8-1.0g/L, FeSO₄0.03-0.05g/L, connecing bacterium amount is 5-10%, is fermented after connecing bacterium, the initial pH value 6.5- of fermentation 7.5,20-40 DEG C of temperature, revolving speed 150-250r/min, ventilation ratio are 1:0.6-1.6, when seeding tank fermentation 30-50 is small, bacterium Body growth is in logarithmic phase, and the fermentation liquid in seeding tank is added to the relaying of the 3000L fermentor equipped with fermentation medium at this time Supervention ferment, fermentation medium: mung bean sprouts 50.0-80.0g/L(is cooked into bean sprout juice), potato starch 20.0-30.0g/L, sucrose 8.0-12.0g/L, yeast extract 3.0-5.0g/L, KCl 0.5-1.0g/L, MgSO₄·7H₂O 0.3-0.5g/L, NaH₂PO₄ 0.3-0.5g/L, CaCl₂·2H₂O 0.1-0.3g/L, pH 6.5-7.8, the same seeding tank of control condition are cultivated 64-96 hours, when When thallus sporulation 90-95%, terminates fermentation, obtain saccharomycetes to make fermentation liquid；After passing it through film concentration, then middle low temperature is dry Dry, after being crushed, cell concentration >=10,000,000,000 CFU/g solid powders is spare；

Step 5: the preparation of colloid bacillus cereus: by the colloid bacillus cereus strain inoculated being stored under -4 DEG C of environment to beef 30 DEG C of culture 48h, picking single colonie are seeded in beef extract-peptone fluid nutrient medium on peptone plate, beef extract-peptone Culture medium: beef extract 3.0g/L, peptone 10.0g/L, sodium chloride 5.0g/L, pH 7.2-7.6, the ring for being 18-35 DEG C in temperature After cultivating for 24 hours under border, required inoculation liquid is obtained；Inoculation liquid is inoculated in the 300L seeding tank equipped with seed culture medium, seed Culture medium: glucose 8.0-10.0g/L, peptone 8.0-15.0g/L, yeast extract 3.0-5.0g/L, sucrose 3.0-5.0g/L, ox Meat extract 2.0-5.0g/L, NaH₂PO₄0.2-0.5g/L, MgSO₄·7H₂O 0.2-0.5g/L, connecing bacterium amount is 5-10%, after connecing bacterium It ferments, the initial pH value 6.8-7.5 of fermentation, 20-40 DEG C of temperature, revolving speed 150-250r/min, ventilation ratio are 1:0.6- 1.6, when seeding tank fermentation 30-50 is small, thalli growth is in logarithmic phase, is added to the fermentation liquid in seeding tank is equipped at this time The 3000L fermentor of fermentation medium relays supervention ferment, fermentation medium: corn pulp 20.0-30.0g/L, beancake powder 20.0- 30.0g/L, yeast powder 3.0-5.0g/L, MgSO₄·7H₂O 0.3-0.5g/L, NaH₂PO₄0.2-0.5g/L, CaCl₂·2H₂ O.2-0.5g/L, K₂HPO₄0.3-0.5g/L, pH 6.5-7.8, the same seeding tank of control condition are cultivated 64-96 hours, work as thallus When sporulation 90-95%, terminates fermentation, obtain colloid bacillus cereus fermentation liquid；Pass it through film concentration after, then in low temperature Dry, after being crushed, cell concentration >=5,000,000,000 CFU/g solid powders is spare；

Step 6: microbial bacterial agent prepare: by the bacillus subtilis of above-mentioned preparation, bacillus licheniformis, bacillus laterosporus, Saccharomycete, colloid bacillus cereus solid thalli powder are mixed according to the weight ratio of design, spare；

Step 7: the preparation of microorganism adsorption material: 0.2-5 parts of potassium fulvates, 1-2.5 parts of humic acids, 0.2-2 parts of amino acid fertilizers Material, microelement in 0.01-0.1 parts, 0.2-2 parts of modified biomass charcoals, 0.05-0.1 parts of peptones according to design parts by weight Volume is spare than being uniformly mixed；

Step 8: the absorption and embedding of microorganism: concentrated biological fermentation liquid being first uniformly mixed with prepared microbial bacteria, so It is uniformly mixed again with spare microorganism adsorption material afterwards, it is spare；

Since humic acid and modified biomass charcoal play the role of absorption and embedding to microorganism, microorganism and chemical raw material are reduced Contact, keep functional microorganism activity and the extension function microorganism shelf-life；

Step 9: the embedding method of chemical fertilizer raw material: by remaining potassium fulvate, humic acid, amino acid fertilizer, modified biomass Charcoal, peptone and chemical fertilizer raw material: potassium nitrate, urea, potassium sulfate, complete water-soluble ammonium phosphate, potassium hydrogen phosphate, middle microelement are according to setting The weight quota of meter is spare than being uniformly mixed；

It, can be former by most chemistry since potassium fulvate, amino acid fertilizer and peptone are easy to the sticky feature of moisture absorption Material is embedded, and the buffer action with microorganism is played, and is further conducive to the activity and extension function that keep functional microorganism The microorganism shelf-life；

Step 10: the preparation of the high organic complex microorganism punching property applied fertilizer: by step 8 absorption and embedded microbial bacteria Agent composition is uniformly mixed with by the embedded chemical fertilizer raw material etc. of step 9, can be prepared by the punching of high organic complex microorganism The property applied fertilizer.

3. a kind of high organic complex microorganism flushing and spreading fertilizer according to claim 1, it has the original of following parts by weight Material composition: 65 parts of potassium fulvate, 5 parts of potassium nitrate, 3 parts of urea, 3 parts of potassium sulfate, 1 part of monoammonium phosphate, 1 part of dipotassium hydrogen phosphate, 5 parts of humic acid, 10 parts of amino acid fertilizer, 0.5 part of peptone, 0.5 part of concentrated biological fermentation liquid, 2 parts of modified biomass charcoal, micro- life 4 parts of object microbial inoculum, 1 part of middle microelement；The middle microelement is amino acid chelated calcium, magnesium sulfate, zinc sulfate, borax, ammonia Base acid chelated iron, cerium nitric acid rare earth are mixed according to 5 parts: 5 parts: 5 parts: 5 parts: 1 part: 0.1 part of mass ratio；The microorganism Microbial inoculum is for bacillus subtilis, bacillus licheniformis, bacillus laterosporus, saccharomycete and colloid bacillus cereus according to bacterium number than 5 Part: 5 parts: 5 parts: 1 part: 1 parts carry out uniformly mixed, and the registration number of bacillus subtilis is CICC 10275, bacillus licheniformis Registration number is ATCC11946, bacillus laterosporus ATCC10249, candida utili bacterium ATCC22023, colloid bacillus cereus Registration number is GIM1.16, effective bacterial content >=10,000,000,000 CFU/g of the microbial bacterial agent；The complete water-soluble ammonium phosphate is phosphoric acid one Ammonium；The potassium hydrogen phosphate is potassium dihydrogen phosphate.

4. a kind of high organic complex microorganism flushing and spreading fertilizer according to claim 1, it has the original of following parts by weight Material composition: 60 parts of potassium fulvate, 15 parts of potassium nitrate, 2 parts of urea, 4 parts of potassium sulfate, 5 parts of Diammonium phosphate (DAP), 2 parts of potassium dihydrogen phosphate, 2 parts of humic acid, 5 parts of amino acid fertilizer, 0.5 part of peptone, 0.5 part of concentrated biological fermentation liquid, 2 parts of modified biomass charcoal, micro- life 1 part of object microbial inoculum, 1 part of middle microelement；The middle microelement is amino acid chelated calcium, magnesium sulfate, zinc sulfate, borax, ammonia Base acid chelated iron, cerium nitric acid rare earth are mixed according to mass ratio 2:2:2:2:1:0.05；The microbial bacterial agent is withered grass Bacillus, bacillus licheniformis, bacillus laterosporus, saccharomycete, colloid bacillus cereus are carried out according to bacterium number ratio 2:2:2:1:1 It is uniformly mixed, the registration number of bacillus subtilis is CICC10275, and bacillus licheniformis registration number is ATCC11946, side spore bud Spore bacillus ATCC10249, candida utili bacterium ATCC22023, colloid bacillus cereus registration number are GIM1.16, the microorganism Effective bacterial content of microbial inoculum >=10,000,000,000 CFU/g；The complete water-soluble ammonium phosphate is Diammonium phosphate (DAP)；The potassium hydrogen phosphate is phosphoric acid hydrogen Dipotassium.

5. a kind of high organic complex microorganism flushing and spreading fertilizer described in -4 according to claim 1, it is characterised in that: the production Experimental program and application effect of a kind of high organic complex microorganism flushing and spreading fertilizer as capsicum top dressing.

6. a kind of high organic complex microorganism flushing and spreading fertilizer described in -3,5 according to claim 1, it is characterised in that: the life Experimental program and application effect of a kind of high organic complex microorganism flushing and spreading fertilizer produced as eggplant top dressing.