CN1685835A - Microorganism plant growth regulating agent and its preparation method - Google Patents
Microorganism plant growth regulating agent and its preparation method Download PDFInfo
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- CN1685835A CN1685835A CN 200510039127 CN200510039127A CN1685835A CN 1685835 A CN1685835 A CN 1685835A CN 200510039127 CN200510039127 CN 200510039127 CN 200510039127 A CN200510039127 A CN 200510039127A CN 1685835 A CN1685835 A CN 1685835A
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- 238000002360 preparation method Methods 0.000 title claims description 16
- 244000005700 microbiome Species 0.000 title claims description 10
- 230000008635 plant growth Effects 0.000 title claims description 9
- 230000001105 regulatory effect Effects 0.000 title claims description 9
- 239000000843 powder Substances 0.000 claims abstract description 39
- 235000015097 nutrients Nutrition 0.000 claims abstract description 20
- 239000005648 plant growth regulator Substances 0.000 claims abstract description 16
- 239000001963 growth medium Substances 0.000 claims abstract description 14
- 210000004369 blood Anatomy 0.000 claims abstract description 6
- 239000008280 blood Substances 0.000 claims abstract description 6
- 241001465754 Metazoa Species 0.000 claims abstract description 3
- 238000000855 fermentation Methods 0.000 claims description 27
- 230000004151 fermentation Effects 0.000 claims description 27
- 239000007787 solid Substances 0.000 claims description 20
- 239000007788 liquid Substances 0.000 claims description 19
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 15
- 229910052799 carbon Inorganic materials 0.000 claims description 15
- 239000002609 medium Substances 0.000 claims description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 239000003795 chemical substances by application Substances 0.000 claims description 8
- 241000223260 Trichoderma harzianum Species 0.000 claims description 7
- 238000011081 inoculation Methods 0.000 claims description 7
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 6
- 208000032843 Hemorrhage Diseases 0.000 claims description 4
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 claims description 4
- 229960002897 heparin Drugs 0.000 claims description 4
- 229920000669 heparin Polymers 0.000 claims description 4
- 239000001509 sodium citrate Substances 0.000 claims description 4
- 238000005406 washing Methods 0.000 claims description 4
- 229910052700 potassium Inorganic materials 0.000 claims description 3
- HPOKESDSMZRZLC-UHFFFAOYSA-N propan-2-one;hydrochloride Chemical compound Cl.CC(C)=O HPOKESDSMZRZLC-UHFFFAOYSA-N 0.000 claims description 3
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 238000012545 processing Methods 0.000 claims description 2
- 239000001054 red pigment Substances 0.000 claims description 2
- 210000002966 serum Anatomy 0.000 claims description 2
- 238000004148 unit process Methods 0.000 claims 2
- 240000008042 Zea mays Species 0.000 abstract description 6
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 abstract description 6
- 235000002017 Zea mays subsp mays Nutrition 0.000 abstract description 6
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- 241000209140 Triticum Species 0.000 abstract description 5
- 235000021307 Triticum Nutrition 0.000 abstract description 5
- 239000010902 straw Substances 0.000 abstract description 5
- 235000013399 edible fruits Nutrition 0.000 abstract description 4
- 235000013311 vegetables Nutrition 0.000 abstract description 4
- 244000241257 Cucumis melo Species 0.000 abstract description 3
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 abstract description 3
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 abstract description 3
- 240000007594 Oryza sativa Species 0.000 abstract description 2
- 235000007164 Oryza sativa Nutrition 0.000 abstract description 2
- 235000009566 rice Nutrition 0.000 abstract description 2
- 241000272496 Galliformes Species 0.000 abstract 1
- 230000000813 microbial effect Effects 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 239000002689 soil Substances 0.000 description 6
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- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 4
- 238000001035 drying Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
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- 239000010905 bagasse Substances 0.000 description 3
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- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 230000000853 biopesticidal effect Effects 0.000 description 2
- 210000000601 blood cell Anatomy 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
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- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 2
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- 239000000126 substance Substances 0.000 description 2
- 108010059892 Cellulase Proteins 0.000 description 1
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- 108090000790 Enzymes Proteins 0.000 description 1
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- 241000006351 Leucophyllum frutescens Species 0.000 description 1
- 102000004317 Lyases Human genes 0.000 description 1
- 108090000856 Lyases Proteins 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
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- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241000223259 Trichoderma Species 0.000 description 1
- 241000082085 Verticillium <Phyllachorales> Species 0.000 description 1
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- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
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- 239000003899 bactericide agent Substances 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
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- 229940106157 cellulase Drugs 0.000 description 1
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- 229940088598 enzyme Drugs 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
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- 230000035931 haemagglutination Effects 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 239000003415 peat Substances 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 238000003307 slaughter Methods 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
- 229940038773 trisodium citrate Drugs 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
A microbial plant growth regulator for rice, wheat, corn, vegetables, melon, fruit tree, etc is prepared from Harzi Trichodema through fermenting in the culture medium which is prepared from wheat straw, corn stalk or cob, cane dregs, protein powder prepared from the fresh blood of domestic animal or fowls, and other nutrients. It can increase the yield of crops by 16-21%.
Description
One, technical field
The present invention relates to a kind of biological plant growth regulator and preparation method thereof, be particularly related to biological plant growth regulator that obtains by fermentation by known microorganisms and preparation method thereof, specifically microorganism plant growth regulating agent for preparing by the Trichoderma harzianum fermentation and preparation method thereof.
Two, background technology
Wood mould (Trichoderma.spp) belongs to the Fungi Imperfecti Moniliales, Moniliaceae fungi (Troiosporae) is a kind of ubiquitous fungi, is common in the soil, be the important group part of edaphon, also see in plant residue and the animal wastes.Enclose and seed from plant rhizosphere, leaf, the bulb surface often can be separated and obtained.Wooden mould have active very strong cellulase and other catabolic enzymes, wooden simultaneously mould still bacterial parasite or the antagonism bacterium of various plants pathogen, as far back as 1981, the wooden removing mildew that just will be used to prevent and treat the fruit trees in plum line silver leaf had important economic implications in Western European countries' commercialization production.
The material that Trichoderma harzianum produced can suppress growth of pathogenic bacteria effectively.Particularly eliminate the cell wall of pathogen, make pathogen be difficult for producing the disease resistance verticillium wilt by lyases.Therefore, this technology can be used for the control of multiple diseases, improves the quality of thing, has broad application prospects.
In the prior art, the Trichoderma harzianum fermentation is used for preparing biopesticide, particularly biological bactericide more.
The environmental pollution and the ecological disruption of China at present caused enormous economic loss.Calculate according to the World Bank, the loss that the pollution of the empty G﹠W of China causes has accounted for 8% of GDP then, and the calculation of measured data of the Chinese Academy of Sciences shows, the development cost that environmental pollution makes China than world average level height 7%, the loss that environmental pollution and ecological disruption cause accounts for 15% (" Science and Technology Daily " on January 26th, 2005 the 3rd edition) of GDP.
China's agricultural production has be unable to do without chemical plant growth regulator and chemical pesticide, and it is improper that its production and use have more, and this is contaminated environment, destruction ecology not only, and polluted agricultural product, has influence on the healthy of people directly or indirectly.Develop can yet be regarded as a kind of important component part of the strategy of sustainable development of biological plant growth regulator and biopesticide.
Three, summary of the invention
This microorganism plant growth regulating agent is liquid microbe plant growth regulator or the solid microbe plant growth regulator that is obtained by fermented and cultured by Trichoderma harzianum, and problem to be solved is to select proper culture medium and the fermented and cultured on this medium.
The present invention selects the nitrogenous source of haemoglobin powder as medium.Select wheat straw, corn straw, corncob or bagasse drying, pulverize the back as culture medium carbon source (calling the carbon source powder in the following text), obtaining liq medium then when getting its juice, when getting its powder, then prepare solid culture medium, after sterilization treatment, inoculate respectively, ferment.It is the liquid microbe plant growth regulator after separating that the zymotic fluid that obtains is cultivated in the liquid medium within top fermentation; Cultivating the solid content that obtains in the solid culture top fermentation is the solid microbe plant growth regulator.
Described haemoglobin powder is that, washing clear through collapse due to massive hemorrhage by the blood of poultry, domestic animal, collapse due to massive hemorrhage red pigment, separation and dry the processing obtain, make hemagglutination precipitation remove serum with sodium citrate or heparin, slough ferroheme in the blood cell with hydrochloric acid acetone through washing, after separating.Separation, drying obtain the haemoglobin powder at last, to replace the peptone of normally used costliness.
The preparation of liquid nutrient medium and fermented and cultured:
Get a certain amount of carbon source powder and add suitable quantity of water decoction 0.5～1 hour, its juice is got in the separation of cooling back, adds a certain amount of nutritive element again, and last dilute with water is settled to certain volume, makes the content relative fixed of each component.
The concrete content of each component following (percentage by weight, down together):
Carbon source powder juice (in the carbon source powder) 2.5～3.5%
Haemoglobin powder 0.1～0.3%
FeSO
4 0.01～0.03％
NaNO
3 0.1～0.7％
MgSO
4 0.1～0.3％
K
2HPO
4 0.1～0.2％
KH
2PO
4 0.05～0.2％
Water surplus.
Above liquid nutrient medium is inoculated behind autoclaving, in 25～28 ℃ of condition bottom fermentations 5～7 days.Just obtain the liquid microbe plant growth regulator after the isolated by filtration.
Preferred carbon source powder 3% (getting its juice), haemoglobin powder 0.2%, FeSO
40.02%, NaNO
30.4%, MgSO
40.2%, K
2HPO
40.15%, KH
2PO
40.1%, surplus was the liquid nutrient medium of water, in 26～28 ℃ of condition bottom fermentations 5～7 days.
The preparation of solid culture medium and fermented and cultured:
Get carbon source powder and haemoglobin powder with the nutrient solution mixing and stirring, be hand and pinch agglomerating, the openhanded shape that scatters, claim solid culture medium, each component content is as follows:
Carbon source powder 55～75%
Haemoglobin powder 10～20%
The nutrient solution surplus.
Described nutrient solution is to contain FeSO in every premium on currency
40.1～0.3g, NaNO
31～7g, MgSO
41～3g, K
2HPO
41～2g, KH
2PO
40.5～2g.
Above solid culture medium is inoculated behind autoclaving, 20～25 ℃ of conditions fermentation 12～18 days, perhaps in 25～28 ℃ of condition bottom fermentations 5～10 days, stirred once every day between yeast phase, perhaps places concussion fermentation on the oscillator, 70～90 times/min of concussion frequency.It after the fermentation ends solid microbe plant growth regulator.
Preferred carbon source powder 60～70%, haemoglobin powder 12～18%, surplus were the solid culture medium of nutrient solution, in 26～28 ℃ of condition bottom fermentations 7～9 days.
Preferred nutrient component is to contain FeSO in every premium on currency
40.2g, NaNO
34g, MgSO
42g, K
2HPO
41.5g, KH
2PO
41g.
The carbon source of medium and nitrogenous source raw material sources are abundant among the present invention, and are cheap, whole production technology green, environmental protection, pollution-free.
(50～100 times) can be used as foliage fertilizer and directly spray on crop blade face or cane behind this liquid plant growth regulating dilution agent, impose in the soil after also can diluting, and execute twice in every month, each mu consumption 25～35Kg.
This solid plant growth regulator can be directly and seed mix and stir and impose in the soil, perhaps add auxiliary material and make particle and use mu consumption 25～35Kg.
This microorganism plant growth regulating agent both can use in sowing time, also can be used as the blade face and used, and was not only applicable to cereal crops such as paddy rice, wheat, corn, also was applicable to melon and fruit, vegetables use, especially was fit to booth vegetable.Studies show that this microorganism can promote absorption, the utilization of crop to N, P, K in the soil, can prevent simultaneously crop that the disease from soil is infected, improve soil structure, so obvious effect of increasing production, to cereal crops, particularly corn yield increasing more than 16%, the vegetables volume increase reaches 21%, and particularly the volume increase of melon and fruit class reaches 26%.
Four, embodiment
1, the preparation of carbon source powder
Get wheat straw, corn straw, corncob or bagasse and pulverize after drying, cross 200 mesh sieves, standby.
2, the preparation of haemoglobin powder
In basin, put trisodium citrate or heparin, in the blood amount, be 0.5～1% of blood weight, put into the blood of the poultry or livestock of slaughtering then, left standstill after shaking gently 1～2 hour, siphon or the centrifugal supernatant that goes, its blood cell washs with physiological saline, clear liquid is removed with preceding method in each washing back, behind the triplicate, stirs in 1: 1～1.5 ratio adding distil waters, under 0～10 ℃ of condition, left standstill 1 hour, promptly add 4～5 times of acetone that contain 2～5% hydrochloric acid, fully stir, filter, slough ferroheme, sediment is a haemoglobin on the filter cloth, drying, pulverize the haemoglobin powder.Surplus ferroheme acetone-aqueous solution reclaims acetone through decompression distillation.
3, the preparation of nutrient solution
In 1 premium on currency, add FeSO
40.15～0.25g, MgSO
41.5～2.5g, NaNO
32～5g, K
2HPO
41～2g, KH
2PO
40.5～2g stirs.
4, the preparation of liquid microbe plant growth regulator
Get carbon source powder 30g and add 200～300ml water boil and filter after 40 minutes, in filtrate, add haemoglobin powder 2g, FeSO
40.2g, NaNO
34g, MgSO
42g, K
2HPO
41.5g, KH
2PO
41g is settled to 1000ml with distilled water diluting, transfers pH5.8～6.8, the fermentation tank of packing into, and autoclaving is handled.Inoculation does not have the spore liquid 1ml of assorted bacterium then, contains spore 1 * 10 approximately
7Individual, in 25～28 ℃ of condition bottom fermentations 5～7 days, speed of agitator was 80r/min, and oxygen supply is 160Pa.Isolated by filtration after fermentation is finished, zymotic fluid promptly is a liquid microbe fertilizer.
5, the preparation of solid microbe fertilizer
Get carbon source powder 650g, haemoglobin powder 150g, get the nutrient solution 200g of above-mentioned 3 preparations, modulate the 1000g solid culture medium, be hand and pinch agglomerating, the openhanded state that can scatter, inoculation does not have assorted bacterium spore liquid 60ml behind the autoclaving, contains spore about 6 * 10
8Individual, in 25～28 ℃ of fermentations 7～9 days, stirred once every day in the fermentation process, and look moisture situation and spread sterile water once in per 2～3 days, keeps humidity.Promptly get the solid microbe plant growth regulator after the fermentation ends.Can directly use, also can add milled powders of cornstalk or bagasse powder or peat dust and be mixed and made into the particle use.
Claims (7)
1, a kind of microorganism plant growth regulating agent is characterized in that: the liquid microbe plant growth regulator or the solid microbe plant growth regulator that are obtained by fermented and cultured by Trichoderma harzianum.
2, a kind of method for preparing microorganism plant growth regulating agent by the Trichoderma harzianum fermentation, comprise preparation, sterilization, inoculation and the fermentation of liquid nutrient medium and separate each unit process, it is characterized in that: inoculation back was in 25～28 ℃ of condition bottom fermentations 5～7 days on following liquid nutrient medium; Described liquid nutrient medium carbonaceous sources powder juice (in the carbon source powder) 2.5～3.5%, haemoglobin powder 0.1～0.3%, FeSO
40.01 NaNO～0.03%,
30.1 MgSO～0.7%,
40.1 K～0.3%,
2HPO
40.1 KH～0.2%,
2PO
40.05～0.2%, surplus is a water.
3, method according to claim 2 is characterized in that: inoculation back was in 26～28 ℃ of condition bottom fermentations 5～7 days on following liquid nutrient medium; Described liquid nutrient medium carbonaceous sources powder juice (in the carbon source powder) 3%, haemoglobin powder 0.2%, FeSO
40.02%, NaNO
30.4%, MgSO
40.2%, K
2HPO
40.15%, KH
2PO
40.1%, surplus is a water.
4, a kind of method for preparing microorganism plant growth regulating agent by the Trichoderma harzianum fermentation, each unit process of preparation, sterilization, inoculation and fermentation that comprises solid culture medium, it is characterized in that: inoculation back is in 20～25 ℃ of condition bottom fermentations 12～18 days, perhaps in 25～28 ℃ of condition bottom fermentations 5～10 days on following solid culture medium; Described solid culture medium carbonaceous sources powder 55～75%, haemoglobin powder 10～20%, surplus is a nutrient solution, described nutrient solution is to contain FeSO in every premium on currency
40.1～0.3g, NaNO
31～7g, MgSO
41～3g, K
2HPO
41～2g, KH
2PO
40.5～2g.
5, method according to claim 4, it is characterized in that: on following solid culture medium, be inoculated in 26～28 ℃ of condition bottom fermentations 7～9 days, described solid culture medium carbonaceous sources powder 60～70%, haemoglobin powder 12～18%, surplus are nutrient solution, and described nutrient solution is to contain FeSO in every premium on currency
40.2g, NaNO
34g, MgSO
42g, K
2HPO
41.5g, KH
2PO
41g.
6, used haemoglobin powder is that, washing clear through collapse due to massive hemorrhage by the blood of domestic birds and animals, collapse due to massive hemorrhage red pigment and dry the processing obtain in the described method of claim 2～5, it is characterized in that: slough serum with sodium citrate or heparin, slough ferroheme with hydrochloric acid acetone.
7, the preparation of haemoglobin powder according to claim 6 is characterized in that: sodium citrate or heparin consumption are 0.5～1%, in the hydrochloric acid acetone hydrochloric 2～5%.
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|---|---|---|---|
| CN 200510039127 CN1685835A (en) | 2005-04-25 | 2005-04-25 | Microorganism plant growth regulating agent and its preparation method |
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|---|---|---|---|
| CN 200510039127 CN1685835A (en) | 2005-04-25 | 2005-04-25 | Microorganism plant growth regulating agent and its preparation method |
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|---|---|
| CN1685835A true CN1685835A (en) | 2005-10-26 |
Family
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102732434A (en) * | 2012-06-18 | 2012-10-17 | 中国农业大学 | Trichoderma harzianum strain, and its application in control of potato late blight |
| CN103131641A (en) * | 2013-02-01 | 2013-06-05 | 安徽农业大学 | T.hariamum multivariate fermentation method and application |
| CN105123242A (en) * | 2015-09-18 | 2015-12-09 | 李晓白 | Ecological wintering method for planting passion fruit in temperate area |
| CN107646368A (en) * | 2017-09-08 | 2018-02-02 | 广西深禾丰农业科技发展有限公司 | A kind of engrafting method of pumpkin and "Hami" melon |
| CN109561692A (en) * | 2016-04-04 | 2019-04-02 | 艾登生物技术公司 | The composition to plant with stimulating activity that microorganism generates |
-
2005
- 2005-04-25 CN CN 200510039127 patent/CN1685835A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102732434A (en) * | 2012-06-18 | 2012-10-17 | 中国农业大学 | Trichoderma harzianum strain, and its application in control of potato late blight |
| CN103131641A (en) * | 2013-02-01 | 2013-06-05 | 安徽农业大学 | T.hariamum multivariate fermentation method and application |
| CN103131641B (en) * | 2013-02-01 | 2015-03-18 | 安徽农业大学 | T.hariamum multivariate fermentation method and application |
| CN105123242A (en) * | 2015-09-18 | 2015-12-09 | 李晓白 | Ecological wintering method for planting passion fruit in temperate area |
| CN105123242B (en) * | 2015-09-18 | 2017-06-16 | 李晓白 | A kind of ecological wintering method that passion fruit is planted in Temperate Region in China |
| CN109561692A (en) * | 2016-04-04 | 2019-04-02 | 艾登生物技术公司 | The composition to plant with stimulating activity that microorganism generates |
| CN109561692B (en) * | 2016-04-04 | 2021-06-04 | 提马克农业集团西班牙公司 | Microbe-produced composition having plant-stimulating activity |
| CN107646368A (en) * | 2017-09-08 | 2018-02-02 | 广西深禾丰农业科技发展有限公司 | A kind of engrafting method of pumpkin and "Hami" melon |
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