CN102040403A - Production of trichoderma spp. microbial fertilizer by waste water of kitchen garbage - Google Patents
Production of trichoderma spp. microbial fertilizer by waste water of kitchen garbage Download PDFInfo
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- CN102040403A CN102040403A CN2009100934797A CN200910093479A CN102040403A CN 102040403 A CN102040403 A CN 102040403A CN 2009100934797 A CN2009100934797 A CN 2009100934797A CN 200910093479 A CN200910093479 A CN 200910093479A CN 102040403 A CN102040403 A CN 102040403A
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Abstract
The invention relates to a production method of a trichoderma spp. biocontrol agent, and belongs to technical fields of microbial fertilizers and the resource utilization of waste water. According to the method, grape peel dregs and grain stillages produced during grape wine production are used as raw materials for the production, and a method of solid state fermentation is adopted to produce the trichoderma spp. biocontrol agent. The production process comprises: (1) taking waste water of kitchen garbage as a medium to inoculate trichoderma spp. and preparing liquid seeds by liquid culture; (2) inoculating the liquid seeds to prepare a large number of liquid trichoderma spp. products with waste water of kitchen garbage as raw materials for the fermentation; (3) adding the cultured liquid seeds or the liquid trichoderma spp. products into biomass materials, and performing solid state fermentation to prepare solid trichoderma spp. products; (4) drying the fermented products, adding stabilizing agents and auxiliary agents, pulverizing, and packaging. The process for producing trichoderma spp. microbial fertilizers has low production cost and is environment-friendly; the products can significantly improve the quality of soil environment, increase crop yields, and control various plant diseases caused by fungi. A processing method for effectively processing plenty of high concentration waste water produced during the production of kitchen garbage is simultaneously realized and environmental pollution is reduced.
Description
Technical field
The invention belongs to microbial fertilizer and utilization of wastewater resource technical field.
Technical background
Along with improving constantly of China's constant development of economy and living standards of the people, domestic food and drink had entered the high speed development stage already, the dietary consumption enormous amount of 1,300,000,000 populations, and the changing food waste of Chan Shenging also is on the increase with it.The changing food waste of every day just has more than 100,000 tons, if deal with improperly, easily environment is caused severe contamination; And at the vast rural area of the considerable provinces and cities of China, city suburbs and the vast raising family, farming district adjoined mutually, the peasant generally directly raises livestock and poultry with undressed changing food waste, because changing food waste is putrid and deteriorated easily, causes this mode of utilizing to have higher food safety risk.Big and medium-sized cities such as Beijing, Shanghai, Shijiazhuang, Chongqing, Yinchuan, Ningbo, Jing Dezhen, Shenzhen, Suzhou have been put into effect changing food waste receipts fortune, have been handled relevant " management method " or draft, particularly Xining have been put into effect first standard changing food waste management of China in 2009 local laws and regulations---" Xining changing food waste management rules " at present.The changing food waste treatment plant that present China has runed some middle and small scales in the part city.Because moisture content produces a large amount of high concentrated organic wastewaters more than 90% after the solid-liquid separation in the changing food waste, the COD value is up to more than 10000 mg/litre, even reaches several ten thousand mg/litre.These wastewater treatments very difficulty up to standard consumes surprising.Consider that from another angle the organic matter in the kitchen garbage, waste-water (protein, starch, grease etc.) is the good nutrition substrate of microorganism growth, can make microbiological culture media, the fermentative production microniological proudcts.
Trichoderma (Trichoderma sp.) is imperfect fungi Moniliales fungi on classification position, widely distributed at occurring in nature, and wood is mould to have antagonistic action to multiple important plant pathogenic fungi, is acknowledged as the very effective biological control factor.Find again that in recent years the mould growth to various plants of art plays promoter action, therefore all develop some based on the mould bio-bacterial manure of wood both at home and abroad, in the promotion plant growth, improved the disease resistance of crop.Microbial fertilizer is meant that a class contains the particular formulations of living microorganism, is applied in the agriculture production, can obtain specific fertilizer effect.It can the activating soil nutrient, strengthens soil supplying nutrient capability, stimulates plant growth, improves crop yield and quality, prevents the breeding of harmful bacterium, increases beneficial microorganism quantity in the soil, to improving ecological environment of soil good promoter action is arranged.
The substratum that production method adopted of the Trichoderma of bibliographical information is main nutrient matter source with grain and byproduct thereof.As: (publication number: disclose the production method of a kind of Trichoderma CN 1554242A), its substratum mainly is millet, rice, corn, barley, wheat or the jowar of particle diameter between the 1-5 millimeter to Chinese patent.(publication number: disclose the production method of a kind of Trichoderma CN 1422946A), its raw materials for production mainly are barley, wheat bran, naked oats etc. to Chinese patent.And the method for producing Trichoderma as substratum with kitchen garbage, waste-water yet there are no report.Trichoderma can multiple Mierocrystalline cellulose of metabolism and hemicellulase, can effectively utilize various biomass, as: stalk, rice husk, bagasse, beet pulp etc.Contain organism such as a large amount of protein, pectin, carbohydrate in the kitchen garbage, waste-water, can provide multiple nutrients for the Trichoderma growth.Therefore, produce Trichoderma with kitchen garbage, waste-water and not only can reduce production costs, and can effectively handle high-concentration waste water.
Summary of the invention
The purpose of this invention is to provide a kind of method of utilizing kitchen garbage, waste-water and abandoned biomass (stalk, bagasse, beet pulp, rice husk etc.) to produce wooden mould microbial fertilizer.
The production method of the mould microbial fertilizer of wood of the present invention is as follows:
1, seed preparation: the Trichoderma kind is conventional agricultural bacterial classification as viride (Trichoderma viride), trichoderma harziarum (Trichoderma harziamum), yellow green trichoderma (Trichoderma auxeoviride), koning trichoderma (Trichodermakoningii) etc.In 115 ℃-126 ℃ sterilizations 15-30 minute, Trichoderma kind, inoculum density 10 were inoculated in the cooling back with kitchen garbage, waste-water
6-10
7Individual spore/milliliter substratum in 20-35 ℃, shakes bottle 120-200 rev/min and cultivated 24-48 hour, promptly gets liquid seed.
2, liquid state fermentation: in 115 ℃-126 ℃ sterilizations 15-30 minute, fresh wastewater also can be unsterilised with kitchen garbage, waste-water.The liquid seed of inoculation 2%-20%, in 20-35 ℃, the aerobic cultivation of ventilation is 24-72 hour in the fermentor tank, promptly gets liquid Trichoderma product.
3, solid state fermentation: with abandoned biomass (stalk of pulverizing, bagasse, beet pulp, rice husk etc.) is upholder, cultured liquid seed or liquid Trichoderma product are added in the biomass material according to 2-4 times of weight, stir, in 20-35 ℃, static cultivation 3-15 days, promptly get solid-state Trichoderma product.
4, aftertreatment: liquid Trichoderma product adds microbial-bacterial fertilizer stablizer commonly used and auxiliary agent based on mycelium, and cryopreservation uses as early as possible.Solid-state Trichoderma product after drying is handled, adds microbial-bacterial fertilizer stablizer commonly used and auxiliary agent based on conidium, pulverizes packing.Also can directly be used in agricultural land soil according to the region situation, can increase soil fertility, can improve the soil again, effectively prevent and treat silborne fungal diseases.
Produce wooden mould microbial fertilizer by technical solution of the present invention, it is low to have a production cost, eco-friendly characteristics, and product can obviously improve soil environment quality, improve crop yield, and can prevent and treat multiple fungi plant disease.Find effective treatment process for the efficient a large amount of high concentrated organic wastewaters that produce in the changing food waste production process of handling simultaneously.Below describe enforcement of the present invention in detail by specific embodiment, purpose is to help the reader to understand spirit of the present invention better, but not as to the qualification of the scope of the present invention.
Embodiment 1
Kitchen garbage, waste-water liquid state, solid-state combined ferment are produced the viride microbial fertilizer:
The seed preparation: in 115 ℃ of sterilizations 20 minutes, the viride bacterial classification was inoculated in the cooling back with kitchen garbage, waste-water, and 107 spores of inoculum density/milliliter substratum in 26 ℃, shake bottle and cultivated 36 hours for 150 rev/mins, promptly get liquid seed.
Liquid state fermentation: in 115 ℃ of sterilizations 20 minutes, fresh wastewater also can be unsterilised with kitchen garbage, waste-water.Inoculate 5% liquid seed, in 26 ℃, the aerobic cultivation of ventilation is 36 hours in the fermentor tank, promptly gets liquid trichoderma viride product.
Solid state fermentation: with the rice husk is upholder, and cultured liquid seed or liquid Trichoderma product are added in the rice husk according to 2.5 times of weight, stirs, and in 26 ℃, static cultivation 7 days promptly gets solid-state trichoderma viride product.
Aftertreatment: solid-state trichoderma viride product after drying is handled, adds microbial-bacterial fertilizer stablizer commonly used and auxiliary agent based on conidium, pulverizes packing.The product spore content can reach 6 * 109 spore/grams.
Embodiment 2
Kitchen garbage, waste-water liquid state, solid-state combined ferment are produced the trichoderma harziarum microbial fertilizer:
The seed preparation: in 121 ℃ of sterilizations 15 minutes, trichoderma harziarum bacterial classification, inoculum density 10 were inoculated in the cooling back with kitchen garbage, waste-water
6Individual spore/milliliter substratum in 28 ℃, shakes bottle and cultivated 48 hours for 150 rev/mins, promptly gets liquid seed.
Liquid state fermentation: kitchen garbage, waste-water in 121 ℃ of sterilizations 15 minutes, is inoculated 10% liquid seed, and in 28 ℃, the aerobic cultivation of ventilation is 36 hours in the fermentor tank, promptly gets liquid trichoderma harzianum product.
Solid state fermentation: with the beet pulp is upholder, and cultured liquid seed or liquid Trichoderma product are added in the beet pulp according to 3 times of weight, stirs, and in 28 ℃, the static ventilation cultivated 6 days, promptly got solid-state trichoderma harzianum product.
Aftertreatment: solid-state trichoderma harzianum product after drying is handled, adds microbial-bacterial fertilizer stablizer commonly used and auxiliary agent based on conidium, pulverizes packing.The product spore content reaches 3 * 10
9Individual spore/gram.
Claims (1)
1. method of utilizing kitchen garbage, waste-water to produce wooden mould microbial fertilizer, it is characterized in that, this method is to be that the main medium raw material carries out liquid state, solid state fermentation is produced wooden mould microbial fertilizer with the high concentrated organic wastewater that produces in the changing food waste treating processes, and the production method of the mould microbial fertilizer of described wood comprises the steps:
(1) seed preparation: kitchen garbage, waste-water was sterilized 15-30 minute in 115 ℃-126 ℃, inoculation conventional agricultural Trichoderma kind in cooling back comprises viride (Trichoderma viride), trichoderma harziarum (Trichoderma harziamum), yellow green trichoderma (Trichoderma auxeoviride), koning trichoderma (Trichoderma koningii).Inoculum density 10
6-10
7Individual spore/milliliter substratum in 20-35 ℃, shakes bottle 120-200 rev/min and cultivated 24-48 hour, obtains liquid seed.
(2) liquid state fermentation: in 115 ℃-126 ℃ sterilizations 15-30 minute, fresh wastewater also can be unsterilised with kitchen garbage, waste-water.The liquid seed of inoculation 2%-20%, in 20-35 ℃, the aerobic cultivation of ventilation is 24-72 hour in the fermentor tank, obtains liquid Trichoderma product.
(3) solid state fermentation: with abandoned biomass (stalk of pulverizing, bagasse, beet pulp, rice husk) is upholder, cultured liquid seed or liquid Trichoderma product are added in the biomass material according to 2-4 times of weight, stir, in 20-35 ℃, static cultivation 3-15 days, obtain solid-state Trichoderma product.
(4) aftertreatment: add microbial-bacterial fertilizer stablizer commonly used and auxiliary agent in the liquid Trichoderma product, cryopreservation.After solid-state Trichoderma product drying is handled, add microbial-bacterial fertilizer stablizer commonly used and auxiliary agent, pulverize packing.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105284905A (en) * | 2015-09-26 | 2016-02-03 | 黄山学院 | Preparation method and application of biocontrol agent |
| CN106045621A (en) * | 2016-06-03 | 2016-10-26 | 罗浩 | Food residue oil-water separation recycling method |
| CN107129327A (en) * | 2017-04-11 | 2017-09-05 | 岳阳绿光农业科技有限公司 | Microbial manure and preparation method thereof |
| CN112812973A (en) * | 2021-04-01 | 2021-05-18 | 中农新科(苏州)有机循环研究院有限公司 | Trichoderma fungus culture medium and preparation method thereof |
| CN114349547A (en) * | 2021-12-23 | 2022-04-15 | 浙江华力环境科技有限公司 | Microbial inoculum for efficiently treating organic garbage and method for preparing bio-organic fertilizer by using microbial inoculum |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101204159A (en) * | 2006-12-22 | 2008-06-25 | 中国科学院生态环境研究中心 | Biological control agents for plant diseases extracting from high concentration organic wastewater |
| CN101391911A (en) * | 2008-11-07 | 2009-03-25 | 四川天正化工有限责任公司 | Method for preparing organic fertilizer by using aminonitrile waste liquid |
-
2009
- 2009-10-10 CN CN2009100934797A patent/CN102040403A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101204159A (en) * | 2006-12-22 | 2008-06-25 | 中国科学院生态环境研究中心 | Biological control agents for plant diseases extracting from high concentration organic wastewater |
| CN101391911A (en) * | 2008-11-07 | 2009-03-25 | 四川天正化工有限责任公司 | Method for preparing organic fertilizer by using aminonitrile waste liquid |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105284905A (en) * | 2015-09-26 | 2016-02-03 | 黄山学院 | Preparation method and application of biocontrol agent |
| CN106045621A (en) * | 2016-06-03 | 2016-10-26 | 罗浩 | Food residue oil-water separation recycling method |
| CN107129327A (en) * | 2017-04-11 | 2017-09-05 | 岳阳绿光农业科技有限公司 | Microbial manure and preparation method thereof |
| CN112812973A (en) * | 2021-04-01 | 2021-05-18 | 中农新科(苏州)有机循环研究院有限公司 | Trichoderma fungus culture medium and preparation method thereof |
| CN114349547A (en) * | 2021-12-23 | 2022-04-15 | 浙江华力环境科技有限公司 | Microbial inoculum for efficiently treating organic garbage and method for preparing bio-organic fertilizer by using microbial inoculum |
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Application publication date: 20110504 |