CN106472575A - A kind of preparation method of nursery stock plant growth regulator - Google Patents
A kind of preparation method of nursery stock plant growth regulator Download PDFInfo
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- CN106472575A CN106472575A CN201610795546.XA CN201610795546A CN106472575A CN 106472575 A CN106472575 A CN 106472575A CN 201610795546 A CN201610795546 A CN 201610795546A CN 106472575 A CN106472575 A CN 106472575A
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- plant growth
- growth regulator
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/03—Algae
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Agronomy & Crop Science (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Fertilizers (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a kind of preparation method of nursery stock plant growth regulator, first take wet cyanophyceae, clean, dry, water is added in cyanophyceae, cellulase, protease and sodium citrate, heating enzymolysis, enzyme denaturing, potassium sulfate is added again in enzymolysis solution, potassium dihydrogen phosphate, heating and thermal insulation, after letting cool, feed liquid is concentrated in vacuo, then add cellulose in concentrated solution, agar, stearic acid, Lactose, stirring, it is warming up to 40 50 DEG C of insulations, last addition organic acid in mixing, methyl oleate, lecithin, Microcrystalline Cellulose, hetastarch sodium, stirring, it is warming up to 30 40 DEG C of insulations, let cool, it is spray-dried, obtain final product.The plant growth regulator of the present invention can increase the enzymatic activity of plant, Net Photosynthetic Rate, proline content, decreases mda content, strengthen drought resistance, finally can improve survival rate.
Description
Technical field
The invention belongs to technical field of pesticide is and in particular to a kind of preparation method of nursery stock plant growth regulator.
Background technology
In recent years, with the quickening of afforestation paces, high mountain, distant mountain, Shi Zhishan, drought and barren, the land occupation condition such as high and cold are disliked
The afforestation ratio in scabland area is increasing, nursery stock resistance is required also to improve constantly, particularly the drought resistance to afforestation nursery stock
Require more extensively, more urgently, thus the nursery stock of directive breeding strong drought resistance has become development trend.In nursery stock directive breeding process
In, the technology that nursery stock resistance can be played with regulating and controlling effect has stocking control, water stress to process, the degeneration-resistant forging of seed
The methods such as refining, section root, photoperiod process, chemical regulation and bioelectric detecting.Wherein the phytohormone in chemical regulation is in plant
Although interior content is very micro, in occupation of highly important status in the stress physiology research of plant.
China has carried out substantial amounts of research in terms of cultivating seedlings theory and technology, and achieves certain achievement, to gloomy
Woods is cultivated and serves positive effect.The method for culturing seedlings of past routine will be it is not intended that cultivated nursery stock will be used for where, and only see
Its height of seedling and ground diameter can be up to standard way, do not adapt to the requirement of production development, particularly the evaluation to seedling quality and
Nursery technology seldom considers reproducing area situation, lack of targeted.For solving this problem, fundamentally change current nursery stock
Cultivate the not strong present situation of purposiveness, with reproducing area land occupation condition and management objectives as starting point, directive breeding nursery stock has become one
The trend of kind.
Content of the invention
The technical problem solving：The purpose of the present invention is to overcome the deficiencies in the prior art and provides a kind of nursery stock to be given birth to plant
The preparation method of long regulator, gained plant growth regulator can strengthen drought resistance, finally improves survival rate.
Technical scheme：A kind of preparation method of nursery stock plant growth regulator, comprises the following steps：
Step 1, takes wet cyanophyceae, cleans, dries, and adds water 10-15 part, cellulase in parts by weight in 1 part of cyanophyceae
0.1-0.5 part, proteinase-10 .1-0.5 part and sodium citrate 0.5-1.2 part, heating enzymolysis, enzyme denaturing, obtain enzymolysis solution；
Step 2, in parts by weight, adds potassium sulfate 0.2-0.6 part, potassium dihydrogen phosphate 0.1-0.3 part in enzymolysis solution, plus
Heat is to 80-90 DEG C of insulation 3-5h, after letting cool, concentrated in vacuo by feed liquid, obtains concentrated solution；
Step 3, in parts by weight, adds cellulose 0.4-0.9 part, agar 0.1-0.5 part, stearic acid in concentrated solution
0.3-0.7 part, Lactose 0.2-0.5 part, stirring, it is warming up to 40-50 DEG C of insulation 20-40min, let cool, obtain mixture；
Step 4, in parts by weight, adds organic acid 0.1-0.5 part, methyl oleate 0.05-0.1 part, lecithin in mixing
Fat 0.1-0.5 part, Microcrystalline Cellulose 0.1-0.2 part, hetastarch sodium 0.01-0.05 part, stirring, it is warming up to 30-40 DEG C of guarantor
Warm 20-40min, lets cool, and is spray-dried, obtains final product.
Further, in step 1, enzymatic hydrolysis condition is 40-50 DEG C, 2-4h.
Further, in step 1, enzymolysis is in CO2Carry out in atmosphere, CO2Ventilation is 3-5L/h.
Further, being concentrated into volume-diminished in step 2 is 10-15%.
Further, described cellulose is one of hydroxyethyl cellulose, hydroxypropyl cellulose or carboxymethyl cellulose.
Further, described organic acid is one of tartaric acid, malic acid, alginic acid or citric acid.
Beneficial effect：The plant growth regulator of the present invention can increase the enzymatic activity of plant, Net Photosynthetic Rate, proline contain
Amount, decreases mda content, strengthens drought resistance, finally can improve survival rate.
Specific embodiment
Embodiment 1
A kind of preparation method of nursery stock plant growth regulator, comprises the following steps：
Step 1, takes wet cyanophyceae, cleans, dries, and adds 10 parts of water, cellulase 0.1 in parts by weight in 1 part of cyanophyceae
Part, proteinase-10 .1 part and 0.5 part of sodium citrate, heating enzymolysis, enzyme denaturing, obtain enzymolysis solution；
Step 2, in parts by weight, adds 0.2 part of potassium sulfate, 0.1 part of potassium dihydrogen phosphate in enzymolysis solution, is heated to 80 DEG C
Insulation 5h, after letting cool, feed liquid is concentrated in vacuo, obtain concentrated solution；
Step 3, in parts by weight, adds 0.4 part of cellulose, 0.1 part of agar, 0.3 part of stearic acid, Lactose in concentrated solution
0.2 part, stirring, it is warming up to 40 DEG C of insulation 40min, let cool, obtain mixture；
Step 4, in parts by weight, in mixing add 0.1 part of organic acid, 0.05 part of methyl oleate, 0.1 part of lecithin,
0.1 part of Microcrystalline Cellulose, 0.01 part of hetastarch sodium, stirring, it is warming up to 30 DEG C of insulation 40min, let cool, be spray-dried, that is,
?.
Wherein, in step 1, enzymatic hydrolysis condition is 40 DEG C, 4h, and enzymolysis is in CO2Carry out in atmosphere, CO2Ventilation is 3-5L/h；
Being concentrated into volume-diminished in step 2 is 10%；Described cellulose is hydroxyethyl cellulose；Described organic acid is tartaric acid.
Embodiment 2
A kind of preparation method of nursery stock plant growth regulator, comprises the following steps：
Step 1, takes wet cyanophyceae, cleans, dries, and adds 13 parts of water, cellulase 0.2 in parts by weight in 1 part of cyanophyceae
Part, proteinase-10 .4 part and 0.7 part of sodium citrate, heating enzymolysis, enzyme denaturing, obtain enzymolysis solution；
Step 2, in parts by weight, adds 0.3 part of potassium sulfate, 0.2 part of potassium dihydrogen phosphate in enzymolysis solution, is heated to 85 DEG C
Insulation 4h, after letting cool, feed liquid is concentrated in vacuo, obtain concentrated solution；
Step 3, in parts by weight, adds 0.7 part of cellulose, 0.2 part of agar, 0.5 part of stearic acid, Lactose in concentrated solution
0.3 part, stirring, it is warming up to 45 DEG C of insulation 30min, let cool, obtain mixture；
Step 4, in parts by weight, in mixing add 0.3 part of organic acid, 0.07 part of methyl oleate, 0.4 part of lecithin,
0.1 part of Microcrystalline Cellulose, 0.04 part of hetastarch sodium, stirring, it is warming up to 35 DEG C of insulation 30min, let cool, be spray-dried, that is,
?.
Wherein, in step 1, enzymatic hydrolysis condition is 45 DEG C, 3h, and enzymolysis is in CO2Carry out in atmosphere, CO2Ventilation is 3-5L/h；
Being concentrated into volume-diminished in step 2 is 15%；Described cellulose is hydroxypropyl cellulose；Described organic acid is malic acid.
Embodiment 3
A kind of preparation method of nursery stock plant growth regulator, comprises the following steps：
Step 1, takes wet cyanophyceae, cleans, dries, and adds 14 parts of water, cellulase 0.3 in parts by weight in 1 part of cyanophyceae
Part, proteinase-10 .2 part and 0.9 part of sodium citrate, heating enzymolysis, enzyme denaturing, obtain enzymolysis solution；
Step 2, in parts by weight, adds 0.5 part of potassium sulfate, 0.2 part of potassium dihydrogen phosphate in enzymolysis solution, is heated to 90 DEG C
Insulation 3h, after letting cool, feed liquid is concentrated in vacuo, obtain concentrated solution；
Step 3, in parts by weight, adds 0.7 part of cellulose, 0.4 part of agar, 0.6 part of stearic acid, Lactose in concentrated solution
0.3 part, stirring, it is warming up to 50 DEG C of insulation 20min, let cool, obtain mixture；
Step 4, in parts by weight, in mixing add 0.4 part of organic acid, 0.08 part of methyl oleate, 0.3 part of lecithin,
0.15 part of Microcrystalline Cellulose, 0.04 part of hetastarch sodium, stirring, it is warming up to 30 DEG C of insulation 40min, let cool, be spray-dried, that is,
?.
Wherein, in step 1, enzymatic hydrolysis condition is 50 DEG C, 2h, and enzymolysis is in CO2Carry out in atmosphere, CO2Ventilation is 3-5L/h；
Being concentrated into volume-diminished in step 2 is 15%；Described cellulose is carboxymethyl cellulose；Described organic acid is citric acid.
Embodiment 4
A kind of preparation method of nursery stock plant growth regulator, comprises the following steps：
Step 1, takes wet cyanophyceae, cleans, dries, and adds 15 parts of water, cellulase 0.5 in parts by weight in 1 part of cyanophyceae
Part, proteinase-10 .5 part and 1.2 parts of sodium citrate, heating enzymolysis, enzyme denaturing, obtain enzymolysis solution；
Step 2, in parts by weight, adds 0.6 part of potassium sulfate, 0.3 part of potassium dihydrogen phosphate in enzymolysis solution, is heated to 80 DEG C
Insulation 5h, after letting cool, feed liquid is concentrated in vacuo, obtain concentrated solution；
Step 3, in parts by weight, adds 0.9 part of cellulose, 0.5 part of agar, 0.7 part of stearic acid, Lactose in concentrated solution
0.5 part, stirring, it is warming up to 40 DEG C of insulation 40min, let cool, obtain mixture；
Step 4, in parts by weight, adds 0.5 part of organic acid, 0.1 part of methyl oleate, 0.5 part of lecithin, micro- in mixing
0.2 part of crystalline cellulose, 0.05 part of hetastarch sodium, stirring, it is warming up to 30 DEG C of insulation 40min, let cool, be spray-dried, obtain final product.
Wherein, in step 1, enzymatic hydrolysis condition is 40 DEG C, 4h, and enzymolysis is in CO2Carry out in atmosphere, CO2Ventilation is 3-5L/h；
Being concentrated into volume-diminished in step 2 is 10%；Described cellulose is hydroxyethyl cellulose；Described organic acid is tartaric acid.
Embodiment 5
The present embodiment is with the difference of embodiment 4：It is also added into 0.01 part of ground calcium carbonate in step 3.
Test seeds are Robinia pseudoacacia L., and June carries out accelerating germination sowing, carries out Osmotic treatment by the end of July.Using embodiment 1 to 5 gained system
Agent is carried out spraying process by the concentration of 100mg/L, is compareed with clear water, and each preparation processes 30 plants, 3 repetitions.Using arid
Canopy method process two weeks, select growing way consistent nursery stock mid-August, take Ce Sina Physiology of Drought Resistance biochemical indicator SOD of blade,
CAT, POD, MDA, proline, soluble protein, result is as follows：
| SOD | CAT | POD | MDA | Proline | Soluble protein | |
| Embodiment 1 | 142 | 0.84 | 62 | 0.943 | 143.2 | 7.5 |
| Embodiment 2 | 135 | 0.79 | 59 | 0.921 | 137.5 | 6.9 |
| Embodiment 3 | 130 | 0.81 | 64 | 0.951 | 132.1 | 7.3 |
| Embodiment 4 | 141 | 0.92 | 65 | 0.924 | 145.7 | 7.5 |
| Embodiment 5 | 167 | 0.97 | 90 | 0.892 | 168.9 | 9.2 |
| Comparison | 62 | 0.22 | 25 | 0.072 | 45.7 | 5.8 |
As seen from the above table, the plant growth regulator of the present invention increased the enzymatic activity of Robinia pseudoacacia L., Net Photosynthetic Rate, proline
Content, decreases mda content, finally can improve survival rate.
Claims (6)
1. a kind of preparation method of nursery stock plant growth regulator it is characterised in that：Comprise the following steps：
Step 1, takes wet cyanophyceae, cleans, dries, and adds water 10-15 part, cellulase 0.1- in parts by weight in 1 part of cyanophyceae
0.5 part, proteinase-10 .1-0.5 part and sodium citrate 0.5-1.2 part, heating enzymolysis, enzyme denaturing, obtain enzymolysis solution；
Step 2, in parts by weight, adds potassium sulfate 0.2-0.6 part, potassium dihydrogen phosphate 0.1-0.3 part in enzymolysis solution, is heated to
80-90 DEG C of insulation 3-5h, after letting cool, feed liquid is concentrated in vacuo, obtain concentrated solution；
Step 3, in parts by weight, adds cellulose 0.4-0.9 part, agar 0.1-0.5 part, stearic acid 0.3- in concentrated solution
0.7 part, Lactose 0.2-0.5 part, stirring, it is warming up to 40-50 DEG C of insulation 20-40min, let cool, obtain mixture；
Step 4, in parts by weight, adds organic acid 0.1-0.5 part, methyl oleate 0.05-0.1 part, lecithin in mixing
0.1-0.5 part, Microcrystalline Cellulose 0.1-0.2 part, hetastarch sodium 0.01-0.05 part, stirring, it is warming up to 30-40 DEG C of insulation
20-40min, lets cool, and is spray-dried, obtains final product.
2. nursery stock plant growth regulator according to claim 1 preparation method it is characterised in that：Enzyme in step 1
Solution condition is 40-50 DEG C, 2-4h.
3. nursery stock plant growth regulator according to claim 1 preparation method it is characterised in that：Enzyme in step 1
Solution is in CO2Carry out in atmosphere, CO2Ventilation is 3-5L/h.
4. nursery stock plant growth regulator according to claim 1 preparation method it is characterised in that：Dense in step 2
Being reduced to volume-diminished is 10-15%.
5. nursery stock plant growth regulator according to claim 1 preparation method it is characterised in that：Described cellulose
It is one of hydroxyethyl cellulose, hydroxypropyl cellulose or carboxymethyl cellulose.
6. nursery stock plant growth regulator according to claim 1 preparation method it is characterised in that：Described organic acid
It is one of tartaric acid, malic acid, alginic acid or citric acid.
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| CN201610795546.XA CN106472575A (en) | 2016-08-31 | 2016-08-31 | A kind of preparation method of nursery stock plant growth regulator |
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| CN201610795546.XA CN106472575A (en) | 2016-08-31 | 2016-08-31 | A kind of preparation method of nursery stock plant growth regulator |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1582667A (en) * | 2004-06-08 | 2005-02-23 | 刘及东 | Seed wrapping agent and process, and seeds thereof |
| US20080127695A1 (en) * | 2004-03-30 | 2008-06-05 | Compagnie Financere Et De Participations Roullier | Use of Ulvans as Elicitors of Mechanisms for Nitrogen Absorption and Protein Synthesis |
| CN101580852B (en) * | 2009-05-18 | 2011-08-31 | 北京雷力农用化学有限公司 | Method for preparing algae essence by using enzymatic hydrolysis method |
| CN103436471A (en) * | 2013-08-23 | 2013-12-11 | 福州大用生物应用科技有限公司 | Composite micro-ecological preparation for nursery stock planting |
| CN103694034A (en) * | 2013-12-02 | 2014-04-02 | 沃太能源南通有限公司 | Preparation method of compound fertilizer dedicated to ginkgo trees by using blue-green algae as base material |
-
2016
- 2016-08-31 CN CN201610795546.XA patent/CN106472575A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080127695A1 (en) * | 2004-03-30 | 2008-06-05 | Compagnie Financere Et De Participations Roullier | Use of Ulvans as Elicitors of Mechanisms for Nitrogen Absorption and Protein Synthesis |
| CN1582667A (en) * | 2004-06-08 | 2005-02-23 | 刘及东 | Seed wrapping agent and process, and seeds thereof |
| CN101580852B (en) * | 2009-05-18 | 2011-08-31 | 北京雷力农用化学有限公司 | Method for preparing algae essence by using enzymatic hydrolysis method |
| CN103436471A (en) * | 2013-08-23 | 2013-12-11 | 福州大用生物应用科技有限公司 | Composite micro-ecological preparation for nursery stock planting |
| CN103694034A (en) * | 2013-12-02 | 2014-04-02 | 沃太能源南通有限公司 | Preparation method of compound fertilizer dedicated to ginkgo trees by using blue-green algae as base material |
Non-Patent Citations (6)
| Title |
|---|
| 张洪昌 等: "《植物生长调节剂使用手册 第2版》", 31 January 2015, 中国农业出版社 * |
| 张莉: "《作物抗旱原理概论》", 30 June 2012, 中国农业科学技术出版社 * |
| 杨宏伟 等: "喷施硫酸钾对干旱胁迫下马铃薯生长发育及抗性生理的影响", 《华北农学报》 * |
| 杨红英 等: "黔东南州农委探索抗旱新措施取得成功", 《贵州日报》 * |
| 程健 等: "《天然产物超临界CO2萃取》", 30 June 2009, 中国石化出版社 * |
| 索世虎 等: "《果园用药施肥技术》", 31 October 1996, 河南科学技术出版社 * |
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Application publication date: 20170308 |