CN106172408A - A kind of 5 hydroxytryptamines are in the application alleviated during Brassica campestris L stain evil is coerced - Google Patents
A kind of 5 hydroxytryptamines are in the application alleviated during Brassica campestris L stain evil is coerced Download PDFInfo
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- CN106172408A CN106172408A CN201610560923.1A CN201610560923A CN106172408A CN 106172408 A CN106172408 A CN 106172408A CN 201610560923 A CN201610560923 A CN 201610560923A CN 106172408 A CN106172408 A CN 106172408A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
- A01G13/02—Protective coverings for plants; Coverings for the ground; Devices for laying-out or removing coverings
- A01G13/0256—Ground coverings
- A01G13/0268—Mats or sheets, e.g. nets or fabrics
- A01G13/0275—Films
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- Agronomy & Crop Science (AREA)
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Abstract
The invention discloses a kind of 5 hydroxytryptamines in the application alleviated during Brassica campestris L stain evil is coerced, its step: a) contain aqueous solution 0.1 0.5g/L of 5 hydroxytryptamines according to weight ratio preparation, weigh 5 hydroxytryptamines according to weight ratio and be dissolved in corresponding water with the weight 0.1:1000 0.5:1000 of water；B) spraying the Brassica Napus Seedling blade face of flooding stress, spray at dusk, spraying frequency is that continuous three days, always spraying number of times was three times once a day；C) amount of solution that the most every basin sprays is 50 mL, makes plant blade face moistening uniformly, hides soil and adjacent basin in basin with plastic sheeting when spraying every time, and spray liquid enters soil and the intensive shadow on lung Seedling being sprayed onto in adjacent basin.Easy to implement the method, strong operability, it is possible to alleviate the injury that stain evil is brought to Brassica Napus Seedling, considerably improve the ability of the resistance to stain of Brassica campestris L.
Description
Technical field
The invention belongs to Rape-seed production field, relate more specifically to a kind of 5-hydroxy tryptamine in alleviation Brassica campestris L stain evil is coerced
Purposes.
Background technology
Brassica campestris L is one of the most most important oil crop, is distributed mainly on the Yangtze river basin at China's Brassica campestris L and (accounts for the whole nation
The Brassica campestris L gross area more than 85%), the rotation system of Oryza sativa L.-Brassica campestris L it is mainly in this area, During Rape Growing Periods is by the South Pacific Ocean
Warm moist air and north cold air cross for a long time in the Yangtze river basin impact, and the most unbroken wet weather is up to 10-15 days, and soil accumulated water is tight
Weight, usually causes Brassica campestris L water content to exceed Brassica campestris L normal demand amount, thus causes Brassica campestris L stain harmful take place frequently (Zhang Xuekun, Chen Jie, Wang Han
Medium, the hereditary difference of moisture resistance of cabbage type rape is identified. China's oil crop journal, and 2007,29:98-102).Stain evil again can
Causing rhizosphere anoxia, the harmful substance such as oxygen-derived free radicals that anaerobic respiration approach produces, ethanol, lactyl is to cell damage
(Chen Jie, Zhang Xuekun, Chen Li etc., the rapid screening of cabbage type rape resistance to stain germ plasm resource, China's oil crop journal, 2006,
28:138-143).After rapeseed plants is coerced by stain evil, root system development is bad, and improving activity of root system weakens, and mineral nutrition is supplied not
Foot, plant becomes short and small, and dry-matter accumulation declines；Blade the most gradually yellow, photosynthesis weakens, and photosynthate subtracts
Few, (Wang Kuang, Wang Youzhen etc., the rape flower pod phase, water logging stress was to production element to ultimately result in crop yield decline even death
Impact, Chinese countryside water conservancy and hydropower, 2010,8:83-90).Stain evil can cause the Brassica campestris L underproduction 17%～42.4%, and the whole nation is oily
Colza is planted main region and occurs the area of stain evil to account for the 20% of the gross area throughout the year, thus stain evil has severely impacted Rape-seed production
Stable high yield (Wang Hanzhong, china rape produces and combats a natural disaster mitigation technical manual, Beijing: Chinese agriculture Science Press, 2009:
14-19).Improve Brassica campestris L resistance to stain ability to be particularly important.
Research about the regulation and control of plant resistance to stain ability is not the most at present, and the external source of can passing through reported applies carry
The hormone of high plant resistance to stain ability regulation and control is less, and have correlational study only has melatonin, jasmonic, abscisic acid, therefore finds new
It is extremely urgent that the phytohormone of type improves Brassica campestris L waterlogging tolerance by foliage-spray.The indoles initiateed by tryptophan metabolic pathway are raw
Alkaloids synthesizes, and plays an important role in the growth and development process of plant, and one of them secondary metabolite is 5-hydroxyl color
Amine (serotonin, SER).5-hydroxy tryptamine, is also called serotonin.Can draw from existing research and report, 5-hydroxy tryptamine
Initially find in animal serum the earliest, have another name called serotonin, be widely present in all kinds of mammal tissue, particularly greatly
In cortex matter and nerve synapse, content is very, and it is also a kind of inhibitory neurotransmitter.In peripheral tissues, 5-hydroxy tryptamine is one
Kind of strong vasoconstrictor and smooth muscle contracting stimulant, play a role as critically important neurotransmitter, participates in numerous physiology, OK
For with emotional activity in the middle of, and as medicinal application (Pelagio-in the middle of the treatment of the mental disorder such as depression
Flores R,Ortiz-Castro R,Mendez-Bravo A,Serotonin,a Tryptophan-Derived Signal
Conserved in Plants and Animals,Regulates Root System Architecture Probably
Acting as a Natural Auxin Inhibitor in Arabidopsis thaliana,Plant and Cell
Physiology,2011,52；490-508).In plant, initially from bean discovery 5-hydroxy tryptamine, the most half
In many centuries, all it is found in 20 more than 40 kinds belonged to subsequently.For it, in plant, the research of function is the most immediately
Being in the news, 5-hydroxy tryptamine has almost participated in the every aspect of growth and development of plants, such as the g and D of root, flower development, really
The maturation of reality, the formation of seed, antioxidation, defying age, the defence etc. of biotic, even it has been reported that itself and derivant thereof are
Phytohormone (Ramakrishna A, Giridhar P, the Ravishankar GA, Phytoserotonin:A of a new class
review.Plant Signaling&Behavior2011,6:800-809).Up to now, at the Genes For Plant Tolerance to 5-hydroxy tryptamine
In inverse research, main concentrate its take part in arid, salt damage, disease-resistant etc. coerce responsing reaction (Bowden K, Brown BG,
Batty JE5-Hydroxytryptamine:its occurrence in cowhage.Nature 1954,174:925-
926；Byrne,M.E,"Making leaves？", Current Opinion in Plant Biology 2012,15 (1):
24-30), but for 5-hydroxy tryptamine how to regulate and control not yet to have to these Stress responses and clearly study.It addition, for 5-
Whether hydroxytryptamine has the research of mitigation to still belong to blank plant stain evil.
Summary of the invention
It is an object of the invention to the application that there are provided a kind of 5-hydroxy tryptamine in alleviation Brassica campestris L stain evil is coerced, method is easy
OK, strong operability, it is possible to alleviate the injury that stain evil is brought to Brassica Napus Seedling, considerably improve the ability of the resistance to stain of Brassica campestris L.
In order to realize above-mentioned purpose, the present invention takes techniques below scheme:
A kind of 5-hydroxy tryptamine is in the application alleviated during Brassica campestris L stain evil is coerced, and the structure of described 5-hydroxy tryptamine is:
The steps include:
A) contain the aqueous solution (0.1-0.5g/L) of 5-hydroxy tryptamine according to weight ratio preparation, 5-hydroxy tryptamine is commercially available prod,
Solid, weighs 5-hydroxy tryptamine according to weight ratio and is dissolved in corresponding water with the weight 0.1:1000 0.5:1000 of water.
B) spraying the Brassica Napus Seedling blade face of flooding stress, general (about 18:00) at dusk sprays, and sprays frequency
Rate is once a day, continuous three days, and always spraying number of times is three times, and the amount of solution that the most every basin sprays is 50mL, makes plant blade face
Moistening uniform.Hide soil and adjacent basin in basin with plastic sheeting when spraying, it is to avoid spray liquid enters soil and is sprayed onto phase every time
Intensive shadow on lung Seedling in adjacent basin.
The present invention compared with prior art, has the following advantages and effect:
5-hydroxy tryptamine is applied to alleviate Brassica campestris L stain evil and coerces by the present invention, and using method is simple, workable,
The method can significantly alleviate stain evil and coerce the injury to rapeseed plants, hence it is evident that improve the energy that rapeseed plants opposing stain evil is coerced
Power.Under the conditions of flooding stress, by spraying 5-hydroxy tryptamine solution so that Brassica Napus Seedling stem length, root length, on the ground fresh weight,
Upper dry weight, underground fresh weight and underground dry weight increase respectively compared to increment for not spraying the Brassica Napus Seedling of 5-hydroxy tryptamine
23.7%-51.6%, 19.8%-35.0%, 27.6%-63.6%, 15.2%-28.1% and 6.0%-29.8%.
Detailed description of the invention
Embodiment 1
Below in conjunction with embodiment, the present invention will be further described, material therefor, reagent, such as 5-hydroxy tryptamine, NaCl and
Soil base manure, is commercial goods.
A kind of 5-hydroxy tryptamine, in the application alleviated during Brassica campestris L stain evil is coerced, the steps include:
A, experiment are carried out in Inst. of Oil Crops, Chinese Academy of Agriculture's glass room in JIUYUE, 2015, use potted plant
Test, basin alms bowl is red plastic keg (internal diameter 19cm, high 18cm), takes from Chinese Academy of Agricultural Sciences oil crop for examination soil and grinds
Studying carefully institute's sun to patrol proving ground and test topsoil the evenlyest, wind desiceted soil 3kg contained by every basin, and soil base manure consumption is N 0.15g, P2O5
0.15g and K2O 0.15g.Fill basin after soil mixing, add water, sowing.Every basin sows 10, and thinning after emerging retains 6 strains, respectively
Process water and fertilizer management is consistent.
B, when cabbage type rape grows to 3 leaf, basin alms bowl is put in pond and carries out submerging treatment (soil in water level and basin
Highly consistent).
C, it is divided into 4 groups according to experimental design: matched group (clear water), process group 1 (0.1g/L 5-hydroxy tryptamine), process group 2
(0.2g/L 5-hydroxy tryptamine), process group 3 (0.5g/L 5-hydroxy tryptamine), often group includes 3 basin alms bowls.
Table 1 water and 5-hydroxy tryptamine weight proportion
| Weight ratio | Matched group | Process group 1 | Process group 2 | Process group 3 |
| 5-hydroxy tryptamine | 0 | 0.1 | 0.2 | 0.5 |
| Water | 1000 | 1000 | 1000 | 1000 |
D, spray concretely comprising the following steps of 5-hydroxy tryptamine solution:
A) it is first according to the preparation of above-mentioned (table 1: water and 5-hydroxy tryptamine weight proportion) weight ratio and contains the water-soluble of 5-hydroxy tryptamine
Liquid, 5-hydroxy tryptamine is commercially available prod, and solid weighs corresponding weight according to table 1 water with 5-hydroxy tryptamine weight proportion and is dissolved in phase
In the water answered.
B) spraying the Brassica Napus Seedling blade face of flooding stress, general (about 18:00) at dusk sprays, and sprays frequency
Rate is once a day, continuous three days, and always spraying number of times is three times, and the amount of solution that the most every basin sprays is 50mL, makes plant blade face
Moistening uniform.Hide soil and adjacent basin in basin with plastic sheeting when spraying, it is to avoid spray liquid enters soil and is sprayed onto phase every time
Intensive shadow on lung Seedling in adjacent basin, other manage measure routinely and mode.
E, cancelling submerging treatment, Brassica Napus Seedling restoration ecosystem is sampling and measuring after 5 days.
The mensuration of physical signs: measure the plant height of each experimental group rapeseed plants, aerial parts and under ground portion respectively fresh
Weight, subsequently in 105 DEG C of 30min that complete, 80 DEG C of drying claim its dry weight to constant weight.
The relieving effect that stain evil suffered by Brassica Napus Seedling is coerced by the 5-hydroxy tryptamine of table 1 variable concentrations
After the 5-hydroxy tryptamine solution of 0.1g/L sprays, Brassica Napus Seedling stem length, root length, on the ground fresh weight, on the ground dry weight, underground are fresh
Weight and underground dry weight are compared to being respectively increased 23.7%, 19.8%, 27.6%, 15.2% and 6.0% in contrast；
After the 5-hydroxy tryptamine solution of 0.1g/L sprays, these character add 48.%, 27.9%, 63.6%, 50.9% respectively,
28.1% and 18.2%；After the 5-hydroxy tryptamine solution of 0.5g/L sprays, these character add 51.6%, 35.0% respectively,
62.4%, 55.3%, 25.8% and 29.8%.
Above experimental data illustrates, under flooding stress, foliage-spray 5-hydroxy tryptamine solution can significantly improve Brassica Napus Seedling
Increment, alleviates the injury that flooding stress brings to Brassica Napus Seedling.
Claims (1)
1. 5-hydroxy tryptamine is in the application alleviated during Brassica campestris L stain evil is coerced, and the steps include:
A) contain the aqueous solution 0.1-0.5g/L of 5-hydroxy tryptamine according to weight ratio preparation, weigh 5-hydroxy tryptamine and water according to weight ratio
Weight 0.1:1000 0.5:1000 be dissolved in corresponding water；
B) spraying the Brassica Napus Seedling blade face of flooding stress, spray at dusk, spraying frequency is once a day, continuous three
My god, always spraying number of times is three times；
C) amount of solution that the most every basin sprays is 50 mL, makes plant blade face moistening uniformly, hides with plastic sheeting when spraying every time
Soil and adjacent basin in lid basin, spray liquid enters soil and the intensive shadow on lung Seedling being sprayed onto in adjacent basin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201610560923.1A CN106172408B (en) | 2016-07-15 | 2016-07-15 | A kind of serotonin is alleviating the application in rape stain evil stress |
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| CN201610560923.1A CN106172408B (en) | 2016-07-15 | 2016-07-15 | A kind of serotonin is alleviating the application in rape stain evil stress |
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| CN106172408A true CN106172408A (en) | 2016-12-07 |
| CN106172408B CN106172408B (en) | 2019-02-05 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108848744A (en) * | 2018-06-29 | 2018-11-23 | 华中农业大学 | Synthesis pathway inhibitor is used to enhance the new application of crop waterlogging tolerance |
| CN109496711A (en) * | 2018-12-29 | 2019-03-22 | 中国农业科学院油料作物研究所 | A kind of serotonin is alleviating the application in rape freeze injury |
| CN112352575A (en) * | 2020-10-21 | 2021-02-12 | 浙江大学 | Method for improving tomato fruit setting rate at high temperature by using 5-hydroxytryptamine preparation |
| CN115843808A (en) * | 2022-12-09 | 2023-03-28 | 湖南农业大学 | Hormone combination for improving waterlogging resistance of rape and application thereof |
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| CN101845497A (en) * | 2010-04-09 | 2010-09-29 | 中国农业科学院油料作物研究所 | Molecular marker closely related to rape humidity resistance, preparation method and application thereof |
| JP2015221763A (en) * | 2014-05-23 | 2015-12-10 | 株式会社５−Ｈｔ総合医薬科学研究所 | Plant growth-promoting agent and plant growth-promoting method |
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2016
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Patent Citations (2)
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| CN101845497A (en) * | 2010-04-09 | 2010-09-29 | 中国农业科学院油料作物研究所 | Molecular marker closely related to rape humidity resistance, preparation method and application thereof |
| JP2015221763A (en) * | 2014-05-23 | 2015-12-10 | 株式会社５−Ｈｔ総合医薬科学研究所 | Plant growth-promoting agent and plant growth-promoting method |
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| HARMEET KAUR等: "Regulatory roles of serotonin and melatonin in abiotic stress rolerance in plants", 《PLANT SIGNALING & BEHAVIOR》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108848744A (en) * | 2018-06-29 | 2018-11-23 | 华中农业大学 | Synthesis pathway inhibitor is used to enhance the new application of crop waterlogging tolerance |
| CN109496711A (en) * | 2018-12-29 | 2019-03-22 | 中国农业科学院油料作物研究所 | A kind of serotonin is alleviating the application in rape freeze injury |
| CN112352575A (en) * | 2020-10-21 | 2021-02-12 | 浙江大学 | Method for improving tomato fruit setting rate at high temperature by using 5-hydroxytryptamine preparation |
| CN115843808A (en) * | 2022-12-09 | 2023-03-28 | 湖南农业大学 | Hormone combination for improving waterlogging resistance of rape and application thereof |
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