CN102224786B - Method and application of photosynthetic bacteria culture solution in medicinal material planting - Google Patents
Method and application of photosynthetic bacteria culture solution in medicinal material planting Download PDFInfo
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Abstract
The invention provides a method and application of a photosynthetic bacteria culture solution in medicinal material planting. The method comprises the following steps: diluting the photosynthetic bacteria culture solution; and evenly irrigating soil, soaking seeds, irrigating roots and spraying. By utilizing the method, the medicinal material growth can be promoted; the occurrence of various disasters can be prevented and treated; the problem of manual culture quality reduction caused by utilizing chemical fertilizers and pesticides can be solved; the traditional Chinese medicine extract content can be increased; the content of the effective components in the traditional Chinese medicinal material is increased; the heavy metal content is reduced; the utilization of the Chinese medicinal material is improved; the sustainable utilization of the Chinese medicinal material is realized; the resources are protected; the implementation of GAP (good agriculture practice) in China can be improved; the problem that crop rotation can not be performed in Chinese medicinal material planting can be solved; and the fertilizer amount is saved by one third.
Description
Technical field
The present invention relates to a kind of medicinal material kind method for planting and application, be specially a kind of method and application that the photosynthetic bacteria culture fluid is used for the medicinal material plantation.
Background technology
The modernization of Chinese medicine is the vital task of China's traditional Chinese medicine.The quality of Chinese medicine is the modernization of Chinese medicine, international basis and assurance, comprises two aspects of validity and safety.And the standardization of traditional Chinese medicine production is its source, so the plantation of traditional Chinese medicine must solve two problems: the one, nuisanceless.Be that content of beary metal and persticide residue must be up to standard; The 2nd, the assurance of traditional Chinese medicine inherent quality arranged, promptly active constituent content is up to standard stable.
At present, researchers apply bio-fertilizer on agricultural, and bio-fertilizer has nontoxic, improves the soil, characteristics free from environmental pollution.Photosynthetic bacteria (Photosynthetic Bacteria; Abbreviation PSB) culture fluid promptly is a kind of bio-fertilizer; And compare with other bio-fertilizer; Have more multiple comprehensive effectiveness, it is to be that raw material, active photosynthetic bacteria are bacterial classification with the autogamy medium, a kind of plyability bio-fertilizer that develops through modern biotechnology.Photosynthetic bacteria has biological nitrogen fixation, decomposes the ability of multiple poisonous and harmful substances such as sulphide and agricultural chemicals, chemical fertilizer, thereby improves the nutrition condition of medicinal material.
In the prior art; Existing document and patent report photosynthetic bacteria culture fluid have promote crops grow, improve obvious effects such as output (can make the vegetables raising the output more than 20%, raising the output such as cereal crops such as wheat, paddy rice, corn 10-40%), prevention and elimination of disease and pests, reduction nitrate content; And this bacterial manure is nontoxic, harmless.As: publication number is the Chinese invention patent of CN101597579A, a kind of production method of agricultural photosynthetic bacteria preparation; Publication number is the patent of invention of CN1101498A, utilizes the plant growth regulator and the production method thereof of micro-organisms; Publication number is the Chinese invention patent of CN1386845A, a kind of bacterium for degradating residual agricultural chemical and production method thereof; Publication number is the Chinese invention patent of CN101139233A, active bio fertilizer and preparation method thereof.Scientific paper, Zheng Zhuohui etc., photosynthetic bacteria is to the degradation effect of cabbage heart residue of pesticide, guangdong agricultural science, 2007 the 7th phases; Xia Qing etc., photosynthetic bacteria liquid is to the influence research of pimento growth, Chinese Ecological Agriculture journal, 2006 the 2nd phases; Zhang Jingjie etc. use the influence of photosynthetic bacteria to greenhouse crop growth proterties and output, Hebei agricultural science, 2008 the 2nd phases; Wang Qiuju etc., application process and the Study on mechanism of photosynthetic bacteria on paddy rice, science of soil and manure, 2006 the 1st phases.
Basically in the above-mentioned prior art, grow, improve the report that output, prevention and elimination of disease and pests, reduction nitrate content etc. improve plant growth for what promote crops; There is not the report that photosynthetic bacterium is applied to improve in the traditional Chinese medicine plantation traditional Chinese medicine extract content and active constituent content; Also do not have solve in the traditional Chinese medicine plantation can not crop rotation problem, reduce the report of common fertilizer consumption.
According to Pharmacopoeia of the People's Republic of China regulation, the medicinal material determination of extractives refers to that water or alcohol measures soluble substance in medicinal material and the medicine materical crude slice for solvent.Can be used as an important indicator in the quality standard of medicinal material.Especially the active component of this medicinal material or index property one-tenth are hard to tell or content is very low or adopt when still not having accurate quantitative approach.So what of medicinal material extract, and wherein content of effective can be used as the characteristic index of quality of medicinal material and medicinal substances extract output and quality.And the increase of the increase of active ingredient and extract can improve the availability of medicinal material, the sustainable use of realization medicinal material, resource conservation; Can promote China GAP enforcement, increase economic efficiency greatly.
Summary of the invention
The purpose of this invention is to provide the method that a kind of photosynthetic bacteria culture fluid is used for the medicinal material plantation, this method can improve medicinal material active ingredient and extract content.
For realizing above-mentioned purpose, the scheme that the present invention adopts is: a kind of photosynthetic bacteria culture fluid is used for the method for medicinal material plantation, comprises following plantation step:
1) the photosynthetic bacteria seed culture fluid of access 1/10-1/5 in through the photosynthetic bacteria culture medium of sterilization, under the 2500Lux illumination condition, room temperature, anaerobism were cultivated 3-15 days, and obtaining viable count is 10
8-10
10The photosynthetic bacteria culture fluid of individual/ml.
2) the photosynthetic bacteria culture fluid be diluted with water to original volume 10-100 doubly, combining to water evenly to irrigate with the usage amount of every mu of 5-20kg imposes in the soil that will plant medicinal material.
3) the photosynthetic bacteria culture fluid be diluted with water to original volume 10-100 doubly, seed soaking is 10-60 minute before the sowing.
4) the photosynthetic bacteria culture fluid be diluted with water to original volume 30-200 doubly, final singling or transplant the back, go into the winter before, turned green in 1 year after, with the usage amount of every mu of medicinal material 1-10kg with the filling root that waters.
5) the photosynthetic bacteria culture fluid be diluted with water to original volume 30-200 doubly, evenly spray medicinal material seedling stage 1 time, vegetative period 2 times then, or back 1 time, vegetative period 2 times of turning green in 1 year with the usage amount of every mu of each 1-10kg of medicinal material.
Photosynthetic bacteria is a Rhodospirillum in the described photosynthetic bacteria culture fluid
Rhodospirillium, Rhodopseudomonas
Rhodopseudomonas, red bacterium belongs to
RhodobacterIn any strain bacterium or their combination in any.
Described Rhodospirillum
RhodospirilliumFor Rhodospirillum rubrum (
Rhodospirillium rubrum); Rhodopseudomonas
RhodopseudomonasFor Rhodopseudomonas palustris (
Rhodopseudomonas palustris), Green color rhodopseudomonas (
Rhodopseudomonas viridis), rhodopseudononas sulfovirdis (
Rhodopseudomonas sulfoviridis), red marine pseudomonas (
Rhodopseudomonas marina); Red bacterium belongs to
RhodobacterFor Spherical red antibacterial (
Rhodobacter sphaeroides).
The source of above-mentioned bacterial classification document: Yao Zhuyun, Zhang Zhaoming. phenotypic characteristic of a few strain photosynthetic bacterias and DNA-DNA homology analysis thereof. use and the environmental organism journal 1996,2 (1): 84-89; Zhang Zhaoming, Yang Suping, Zhao Chungui. the isolation identification research of Rhodopseudomonas palustris. University Of Shanxi's journal (natural science edition), 1992, (4): 379-385; Yang Suping, Zhang Zhaoming, Zhao Chungui. separating and evaluation of Green color rhodopseudomonas and rhodopseudononas sulfovirdis. microorganism journal, 1995,35 (2): 91-96; Zhang Zhaoming, Deng Songlu, Zhao Liangqi, etc. the research of separation, evaluation and the physiological property of the research A. Rhodopseudomonas spheroides of Purple Non-sulfur photosynthetic bacteria. University Of Shanxi's journal (natural science edition), 1984, (4): 54-59.
These bacterial classifications all come from Life Science and Technology institute of Shanxi province Taiyuan city University Of Shanxi photosynthetic bacteria research department.
The medium of said suitable photosynthetic bacterium growth can adopt photosynthetic bacteria culture medium of the prior art, the preferred following medium of the present invention, and it consists of:
Sodium acetate 1000-2000mg CaCl
2.2H
2O 50-100 mg
MgSO
4.7 H
2O 100-300 mg EDTA 10-30 mg
Yeast extract 500-1500 mg K
2HPO
4500-1500 mg
(NH
4)
2SO
41000-2000 mg KH
2PO
4 400-1000 mg
FeSO
4.7H
2O 5-15mg trace element solution 1-5 ml
Deionized water 1000-2000 ml pH 6-8
Wherein trace element solution consists of:
H
3BO
3 200-300 mg Na
2MoO
4.2H
2O 50-100 mg
CuSO
4 2-10 mg MnSO
4.4H
2O 150-250 mg
ZnSO
4.7H
2O 20-30 mg deionized water 100-200 ml
Compared with prior art the present invention has following beneficial effect:
The first, the photosynthetic bacteria culture fluid is applied in the traditional Chinese medicine plantation, the characteristic that the performance photosynthetic bacteria transforms remains of pesticide, heavy metal, promotion growth and development of plants and prevention and elimination of disease and pests improves the traditional Chinese medicine quality, for the enforcement of traditional Chinese medicine GAP provides condition.
The second, not only can improve traditional Chinese medicine output, reduce traditional Chinese medicine residue of pesticide, content of beary metal, guaranteed the safety of traditional Chinese medicine; The more important thing is and improved active constituent content and the general quality evaluation index extract content of traditional Chinese medicine, give full play to the effectiveness of photosynthetic bacteria culture fluid.
The 3rd, the innovation on the traditional Chinese medicine culture technique.Adopt the photosynthetic bacteria microbial inoculum to improve the soil, residue of pesticide, heavy-metal residual and harmful microorganism are to the harm and the pollution of traditional Chinese medicine in the elimination soil; Employing photosynthetic bacteria microbial inoculum raising traditional Chinese medicine is disease-resistant, pest-resistant, the ability in degeneration-resistant border, promotes that traditional Chinese medicine grows, and improves output; Adopt the photosynthetic bacteria microbial inoculum to improve traditional Chinese medicine active constituent content and extract content, improve its integrated quality comprehensively.Changed the problem that the traditional Chinese medicine plantation can not continuous cropping.Embodied the advantage of modern Chinese herbal medicine " triple effect (efficient, quick-acting, long-acting), three little (dosage is little, toxicity is little, side effect little), three just (are convenient to store, carry, take) " to a certain extent.Save chemical fertilizer 1/3.
The 4th, the increase of active ingredient and the increase of extract can improve the availability of medicinal material, realize the sustainable use of medicinal material, resource conservation; Can promote China GAP enforcement, increase economic efficiency greatly.
The 5th, photosynthetic bacteria culture fluid raw material sources of the present invention are wide, low price, and growth conditions is controlled easily, and various low molecule organic matters capable of using are mainly sought the photoheterotrophy life as carbon source and photosynthetic hydrogen donor.Can assimilate multiple organic matters such as fatty acid, carbohydrate and aromatic compound; Organic matter capable of using carries out aerobic heterotrophic growth as respiratory substrate under dark condition.Change the mode that it obtains energy with changes in environmental conditions, change the metabolic way of self.Suitability for mass industrialized enforcement.
The application of photosynthetic bacteria culture fluid in improving medicinal material active ingredient and extract content.Improving active ingredient and extract content combines following planting experiment to describe.
Example one, the plantation of Radix Isatidis, leaf is the folium isatidis medicinal material, root is the Radix Isatidis medicinal material.Experiment all repeats 3 times, gets 3 times mean value.
The assay of indigo red carries out according to the content assaying method of indigo red in 2010 editions Pharmacopoeia of People's Republic of China folium isatidiss in the folium isatidis.
The assay of extract is measured according to the hot dipping under ethanol soluble extractives determination method (Chinese Pharmacopoeia appendix
A) item in the folium isatidis, makees solvent with 95% ethanol.
The assay of indigo red carries out according to the content assaying method of indigo red in 2010 editions Pharmacopoeia of People's Republic of China folium isatidiss in the Radix Isatidis.
The mensuration of extract is measured according to the hot dipping under ethanol soluble extractives determination method (Chinese Pharmacopoeia appendix
A) item in the Radix Isatidis, makees solvent with 45% ethanol.
The result: not using the content of indigo red in the folium isatidis of photosynthetic bacteria culture fluid is 0.11 mg/g, and alcohol extract content is 12.9%; The content of indigo red is 0.26 mg/g in the folium isatidis of use photosynthetic bacteria culture fluid, and alcohol extract content is 17.3%.Indigo red content increases by 136.4%, and extract content increases by 34.1%.Not using the content of indigo red in the Radix Isatidis of photosynthetic bacteria culture fluid is 0.0039 mg/g, and alcohol extract content is 37.4%; The content of indigo red is 0.0075 mg/g in the Radix Isatidis of use photosynthetic bacteria culture fluid, and alcohol extract content is 41.9%.Indigo red content increases by 92.3%, and extract content increases by 12.0%.
Example two, the plantation of the root of large-flowered skullcap.Wherein scutelloside carries out according to content of baicalin assay method in 2010 editions Pharmacopoeia of People's Republic of China roots of large-flowered skullcap; Extract content is measured the hot dipping that adopts under 2010 editions Pharmacopoeia of People's Republic of China ethanol soluble extractives determination methods (Chinese Pharmacopoeia appendix
A) item and is measured, and makees solvent with 30% Diluted Alcohol.
The result: do not use that content of baicalin is 136.47mg/g in the root of large-flowered skullcap of photosynthetic bacteria culture fluid, alcohol extract content is 42.6%; Use that content of baicalin is 150.43mg/g in the root of large-flowered skullcap of photosynthetic bacteria culture fluid, alcohol extract content is 47.8%.Content of baicalin increases by 10.23%, and extract content increases by 12.2%.
Example three, the plantation of Radix Codonopsis.Wherein the content of lobetyolin adopts high performance liquid chromatography to carry out, and chromatographic condition is flowing phase: acetonitrile-water (20:80), flow velocity: 1mlmin
﹣ 1, detect wavelength: 267nm, sample size: 10 μ l, chromatographic column: C18 performance liquid chromatographic column.The assay of extract adopts the hot dipping under 2010 editions Pharmacopoeia of People's Republic of China ethanol soluble extractives determination methods (Chinese Pharmacopoeia appendix
A) item to measure, and makees solvent with 45% ethanol.
The result: not using the content of lobetyolin in the Radix Codonopsis of photosynthetic bacteria culture fluid is 0.48 mg/g, and alcohol extract content is 57.4%; The content of lobetyolin is 0.85 mg/g in the Radix Codonopsis of use photosynthetic bacteria culture fluid, and alcohol extract content is 63.8%.Lobetyolin's content increases by 77.1%, and extract content increases by 8.2%.
Embodiment
Embodiment 1
The plantation of Radix Isatidis, folium isatidis
Press following set of dispense system medium:
Sodium acetate 1000-2000mg CaCl
2.2H
2O 50-100 mg
MgSO
4.7 H
2O 100-300 mg EDTA 10-30 mg
Yeast extract 500-1500 mg K
2HPO
4500-1500 mg
(NH
4)
2SO
41000-2000 mg KH
2PO
4 400-1000 mg
FeSO
4.7H
2O 5-15mg trace element solution 1-5 ml
Deionized water 1000-2000 ml pH 6-8
Wherein trace element solution consists of:
H
3BO
3 200-300 mg Na
2MoO
4.2H
2O 50-100 mg
CuSO
4 2-10 mg MnSO
4.4H
2O 150-250 mg
ZnSO
4.7H
2O 20-30 mg deionized water 100-200 ml
In through the photosynthetic bacteria culture medium of sterilization, insert the Spherical red antibacterial seed culture fluid of 1/10 umber, under the 2500Lux illumination condition, room temperature, anaerobism were cultivated 3-15 days, and obtaining viable count is 10
8-10
10The photosynthetic bacteria Spherical red antibacterial culture fluid of individual/ml.
Plough October, and combining winter irrigates the fields uses the photosynthetic bacteria culture fluid irrigated soil that are diluted to 50 times of original volumes, and consumption is 10 kilograms/mu.In mid-April, second sowing except that common seed soaking, was soaked seed 30 minutes with the photosynthetic bacteria culture fluid that is diluted to 100 times of original volumes before the sowing again.The drilling method sowing was emerged in 7-9 days, the back thinning final singling of emerging.After the final singling, the photosynthetic bacteria culture fluid is diluted with water to 200 times of original volume with the filling root of irrigating the fields, consumption is 10 kilograms/mu.The photosynthetic bacteria culture fluid is diluted with water to 100 times of original volume, seedling stage 1 time, vegetative period twice, evenly sprays with the usage amount of every mu of each 5kg of medicinal material.Late October digs and receives Radix Isatidis.Can prevent the generation of downy mildew and root rot, can continuous cropping.Folium isatidis early June, early August receive twice, dig October and receive once before receiving Radix Isatidis again.The a small amount of photosynthetic inoculum of each spray can be prevented going mouldy before the harvesting.205 kilograms of every per mu yield folium isatidis cured leafs, 245 kilograms of Radix Isatidis finished products.All the other are not with using the contrast plantation group of photosynthetic bacteria, and just the various fertilizer of executing reduce to 2/3.Do not use 190 kilograms of the every per mu yield folium isatidis of the control group cured leafs of photosynthetic bacteria culture fluid, 224 kilograms of Radix Isatidis finished products.Folium isatidis output increases by 7.89%, and Radix Isatidis output increases by 9.38%.
The folium isatidis indigo red content that produces is 0.47 mg/g, and alcohol extract content is 20.1%; Not using the control group folium isatidis indigo red content of photosynthetic bacteria is 0.24 mg/g, and alcohol extract content is 17.5%.Indigo red content has increased by 95.8%, alcohol extract content has increased by 14.9%.The Radix Isatidis indigo red content that produces is 0.0064 mg/g, and alcohol extract content is 47.2%; Not using the control group Radix Isatidis indigo red content of photosynthetic bacteria is 0.0041 mg/g, and alcohol extract content is 42.2%, and indigo red content has increased by 56.1%, alcohol extract content has increased by 11.8%.
Embodiment 2
The plantation of Radix Isatidis, folium isatidis
The preparation method of photosynthetic bacteria culture medium is with embodiment 1.
In through the photosynthetic bacteria culture medium of sterilization, insert the Rhodospirillum rubrum seed culture fluid of 1/10 umber, under the 2500Lux illumination condition, room temperature, anaerobism were cultivated 3-15 days, and obtaining viable count is 10
8-10
10The photosynthetic bacteria Rhodospirillum rubrum culture fluid of individual/ml.
Plough October; Combining winter waters uses the photosynthetic bacteria culture fluid irrigated soil that are diluted to 10 times of original volumes, and consumption is 5 kilograms/mu, in mid-April, second sowing; Except that common seed soaking, soaked seed 60 minutes with the photosynthetic bacteria culture fluid that is diluted to 50 times of original volumes again before the sowing.The drilling method sowing was emerged in 7-9 days, the back thinning final singling of emerging.After the final singling, the photosynthetic bacteria culture fluid is diluted with water to 150 times of original volume with the filling root of irrigating the fields, consumption is 5 kilograms/mu.The photosynthetic bacteria culture fluid is diluted with water to 70 times of original volume, seedling stage 1 time, vegetative period twice, evenly sprays with the usage amount of every mu of each 10kg of medicinal material.Late October digs and receives Radix Isatidis.Can prevent the generation of downy mildew and root rot, can continuous cropping.Folium isatidis early June, early August receive twice, and receive once October again.The a small amount of photosynthetic inoculum of each spray can prevent going mouldy 210 kilograms of per mu yield cured leafs, 260 kilograms of Radix Isatidis finished products before the harvesting.All the other are not with using the contrast plantation group of photosynthetic bacteria, and just the various fertilizer of executing reduce to 2/3.Do not use 189 kilograms of the every per mu yield folium isatidis of the control group cured leafs of photosynthetic bacteria culture fluid, 238 kilograms of Radix Isatidis finished products.Folium isatidis output increases by 11.11%, and Radix Isatidis output increases by 9.24%.
Not using the content of indigo red in the folium isatidis of photosynthetic bacteria culture fluid is 0.11 mg/g, and alcohol extract content is 12.9%; The content of indigo red is 0.26 mg/g in the folium isatidis of use photosynthetic bacteria culture fluid, and alcohol extract content is 17.3%.Indigo red content increases by 136.4%, and extract content increases by 34.1%.Not using the content of indigo red in the Radix Isatidis of photosynthetic bacteria culture fluid is 0.0039 mg/g, and alcohol extract content is 37.4%; The content of indigo red is 0.0075 mg/g in the Radix Isatidis of use photosynthetic bacteria culture fluid liquid, and alcohol extract content is 41.9%.Indigo red content increases by 92.3%, and extract content increases by 12.0%.
Embodiment 3
The plantation of the root of large-flowered skullcap.
The preparation method of photosynthetic bacteria culture medium is with embodiment 1.
In through the photosynthetic bacteria culture medium of sterilization, insert the Rhodopseudomonas palustris seed culture fluid of 1/5 umber, under the 2500Lux illumination condition, room temperature, anaerobism were cultivated 3-15 days, and obtaining viable count is 10
8-10
10The photosynthetic bacteria Rhodopseudomonas palustris culture fluid of individual/ml.
Grow seedlings and plough before field and transplant field are all gone into the winter, combining winter waters uses the photosynthetic bacteria culture fluid irrigated soil that are diluted to 100 times of original volumes, and consumption is 20 kilograms/mu.In early April, second sows in spring, and adopts drilling, soaks seed 10 minutes with the photosynthetic bacteria culture fluid that is diluted to 10 times of original volumes before the planting seed.Emerge about 10 days.When seedling grows to 6～8 centimetres high, select the cloudy day to go transplantation of seedlings to big Tanaka, 25～30 * 12～15 centimetres of field planting distances between rows and hills, the ratio that seedlings growing area and transplant field are transplanted area is generally 1:5.After the transplanting, the photosynthetic bacteria culture fluid is diluted with water to 200 times of original volume with the filling root of irrigating the fields, consumption is 7 kilograms/mu.The photosynthetic bacteria culture fluid is diluted with water to 40 times of original volume, seedling stage 1 time, vegetative period twice, evenly sprays with the usage amount of every mu of each 7 kg of medicinal material.Use the photosynthetic bacteria culture fluid that is diluted to 100 times of original volumes with the filling root of irrigating the fields before going into the winter, consumption is 10 kilograms/mu, after turning green 1 year spring, cultivates liquid irrigating root once with irrigating the fields with the photosynthetic bacteria that is diluted to 30 times of original volumes, and consumption is 1 kilogram/mu.The photosynthetic bacteria culture fluid that is diluted with water to 150 times of original volumes evenly sprays, after turning green in 1 year once, twice of vegetative period.Photosynthetic bacteria culture fluid consumption is each 1 kilogram/mu.All the other are not with using the contrast plantation group of photosynthetic bacteria, and just the various fertilizer of executing are reduced to and used 2/3 of photosynthetic bacteria culture fluid soil.Transplanting next year gathers.
395 kilograms of average yield per mus, active ingredient content of baicalin are 146.25mg/g, and alcohol extract content is 46.2%.Do not use 352 kilograms of the control group average yield per mus of photosynthetic bacteria, content of baicalin is 124.36 mg/g, and alcohol extract content is 41.4%.Output increases by 12.22%, and content of baicalin has increased by 17.6%, and extract content improves 11.6%.
Embodiment 4
The plantation of the root of large-flowered skullcap
The preparation method of photosynthetic bacteria culture medium is with embodiment 1.
In through the photosynthetic bacteria culture medium of sterilization, insert the Spherical red antibacterial and the Rhodopseudomonas palustris seed mixture culture fluid of 1/10 umber, under the 2500Lux illumination condition, room temperature, anaerobism were cultivated 3-15 days, and obtaining viable count is 10
8-10
10The bacterium Spherical red antibacterial of individual/ml, the mixed cell culture fluid of Rhodopseudomonas palustris.
Grow seedlings and plough before field and transplant field are all gone into the winter; Combining winter waters uses the photosynthetic bacteria culture fluid irrigated soil that are diluted to 100 times of original volumes; Consumption is 15 kilograms/mu; In early April, second sows in spring, and adopts drilling, soaks seed 50 minutes with the photosynthetic bacteria culture fluid that is diluted to 50 times of original volumes before the planting seed.Emerge about 10 days.When seedling grows to 6～8 centimetres high, select the cloudy day transplantation of seedlings in transplant field, 25～30 * 12～15 centimetres of field planting distances between rows and hills, seedlings growing area is generally 1:5 with the ratio of transplant field transplanting area.After the transplanting, the photosynthetic bacteria culture fluid is diluted with water to 150 times of original volume with the filling root of irrigating the fields, consumption is 5 kilograms/mu.The photosynthetic bacteria culture fluid is diluted with water to 150 times of original volume, seedling stage 1 time, vegetative period twice, evenly sprays with the usage amount of every mu of each 8 kg of medicinal material.Use the photosynthetic bacteria culture fluid that is diluted to 200 times of original volumes with the filling root of irrigating the fields before going into the winter, consumption is 7 kilograms/mu, after turning green 1 year spring, cultivates liquid irrigating root with irrigating the fields with the photosynthetic bacteria that is diluted to 100 times of original volumes, and consumption is 5 kilograms/mu.The photosynthetic bacteria culture fluid that is diluted with water to 150 times of original volumes evenly sprays, after turning green in 1 year once, twice of vegetative period.Photosynthetic bacteria culture fluid consumption is each 5 kilograms/mu.All the other are not with using the contrast plantation group of photosynthetic bacteria, and just the various fertilizer of executing are reduced to and used 2/3 of photosynthetic bacteria culture fluid.Transplanting next year gathers.
Do not use the root of large-flowered skullcap control group average yield per mu 348kg of photosynthetic bacteria culture fluid, content of baicalin is 136.47mg/g, and alcohol extract content is 42.6%; Use the root of large-flowered skullcap control group average yield per mu 389kg of photosynthetic bacteria culture fluid, content of baicalin is 150.43mg/g, and alcohol extract content is 47.8%.Output increases by 11.78%, and content of baicalin increases by 10.23%, and extract content increases by 12.2%.
Embodiment 5
The plantation of Radix Codonopsis
The preparation method of photosynthetic bacteria culture medium is with embodiment 1.
In through the photosynthetic bacteria culture medium of sterilization, insert Rhodospirillum rubrum, Green color rhodopseudomonas and the red marine pseudomonas seed mixture culture fluid of 1/10 umber, under the 2500Lux illumination condition, room temperature, anaerobism were cultivated 3-15 days, and obtaining viable count is 10
8-10
10The photosynthetic inoculum of the mixing of the Rhodospirillum rubrum of individual/ml, Green color rhodopseudomonas and red marine pseudomonas.
Grow seedlings October in autumn, and consumption was 10 kilograms/mu to seeding and growing seedling with irrigating the fields with the photosynthetic bacteria culture fluid irrigated soil that are diluted to 70 times of original volumes in preceding 7 days.With the photosynthetic bacteria culture fluid seed soaking that is diluted to 100 times of original volumes 30 minutes, go into usefulness is diluted to 50 times of original volumes before the winter photosynthetic bacteria culture fluid with the filling root of irrigating the fields before the planting seed, consumption is 7 kilograms/mu.Used the photosynthetic bacteria culture fluid that is diluted to 150 times of original volumes with the filling root of irrigating the fields after turning green in 1 year, consumption is 5 kilograms/mu.Transplant 1 year autumn, transplants preceding 6 days with watering with the photosynthetic bacteria culture fluid irrigation transplanting ground soil that is diluted to 70 times of original volumes, and consumption is 10 kilograms/mu.Before the transplanting, seedling has been dug, rejected no bud head, damage and be completely cured and too immature inferior strain, tied into wisp, with planting with getting.Every mu needs seedling 25-30 kilogram.Transplant the back and use the photosynthetic bacteria culture fluid that is diluted to 120 times of original volumes with the filling root of irrigating the fields, consumption is 7 kilograms/mu.9 centimetres of right and lefts of height of seedling can loosen the soil and hoe up weeds.Feng Hanghou stops to hoe up weeds.When 30 centimetres of heights of seedling, set up support with bamboo bar or branch, make the viticula growth of climbing, both favourable ventilation and penetrating light strengthens photosynthesis, can prevent the excessive and ill worm harm of field humidity again, is a well stimulation of Radix Codonopsis cultivation.Use the photosynthetic bacteria culture fluid that is diluted to 50 times of original volumes with the filling root of irrigating the fields before going into the winter in 1 year, consumption is 10 kilograms/mu.The photosynthetic bacteria culture fluid is diluted to 200 times of original volumes, evenly sprays, and consumption is every mu of each 5kg of medicinal material, medicinal material seedling stage 1 time, vegetative period twice then, after turning green in second, third year once, twice of vegetative period.The 3rd year results, the output of Radix Codonopsis is 255 kilograms every mu.All the other are not with using the contrast plantation group of photosynthetic bacteria, and just the various fertilizer of executing reduce to 2/3.The root rot that is easy to get and rust can alleviate with photosynthetic bacteria.The control group output of not using the photosynthetic bacteria culture fluid is 234 kilograms every mu.Output increases by 8.97%.
The Radix Codonopsis lobetyolin content that produces is 0.52 mg/g, and alcohol extract content is 60.7%; Not using the control group Radix Codonopsis lobetyolin content of photosynthetic bacteria is 0.37 mg/g, and alcohol extract content is 56.1%.Lobetyolin's content increase by 28.8%, alcohol extract content increase by 8.2%.
Embodiment 6
The plantation of Radix Codonopsis
The preparation method of photosynthetic bacteria culture medium is with embodiment 1.
In through the photosynthetic bacteria culture medium of sterilization, insert the rhodopseudononas sulfovirdis seed culture fluid of 1/10 umber, under the 2500Lux illumination condition, room temperature, anaerobism were cultivated 3-15 days, and obtaining viable count is 10
8-10
10The rhodopseudononas sulfovirdis photosynthetic bacteria culture fluid of individual/ml.
Grow seedlings October in autumn, and consumption was 10 kilograms/mu to seeding and growing seedling with irrigating the fields with the photosynthetic bacteria culture fluid irrigated soil that are diluted to 100 times of original volumes in preceding 5 days.With the photosynthetic bacteria culture fluid seed soaking that is diluted to 50 times of original volumes 20 minutes, go into usefulness is diluted to 150 times of original volumes before the winter photosynthetic bacteria culture fluid with the filling root of irrigating the fields before the planting seed, consumption is 3 kilograms/mu.Turned green in 1 year and use the photosynthetic bacteria culture fluid that is diluted to 200 times of original volumes with the filling root of irrigating the fields, consumption is each 10 kilograms/mu.Transplant 1 year autumn, and transplant field is transplanted preceding 6 days with watering with the photosynthetic bacteria culture fluid irrigated soil that are diluted to 50 times of original volumes, and consumption is 20 kilograms/mu.Before the transplanting, seedling has been dug, rejected no bud head, damage and be completely cured and too immature inferior strain, tied into wisp, with planting with getting.Every mu needs seedling 25-30 kilogram.9 centimetres of right and lefts of height of seedling can loosen the soil and hoe up weeds.Feng Hanghou stops to hoe up weeds.When 30 centimetres of heights of seedling, set up support with bamboo bar or branch, make the viticula growth of climbing, both favourable ventilation and penetrating light strengthens photosynthesis, can prevent the excessive and ill worm harm of field humidity again, is a well stimulation of Radix Codonopsis cultivation.Use the photosynthetic bacteria culture fluid that is diluted to 50 times of original volumes with the filling root of irrigating the fields before going into the winter in 1 year, consumption is 5 kilograms/mu.The photosynthetic bacteria culture fluid is diluted to 30 times of original volume, evenly sprays, consumption is every mu of each 3kg of medicinal material, medicinal material seedling stage 1 time, vegetative period twice then, after turning green in second, third year once, twice of vegetative period.The 3rd year results, the output of Radix Codonopsis is 275 kilograms every mu.All the other are not with using the contrast plantation group of photosynthetic bacteria, and just the various fertilizer of executing reduce to 2/3.The root rot that is easy to get and rust can alleviate with photosynthetic inoculum.The control group output of not using the photosynthetic bacteria culture fluid is 229 kilograms every mu.Output increases by 20.1 %.
Not using the content of lobetyolin in the Radix Codonopsis of photosynthetic bacteria culture fluid is 0.48 mg/g, and alcohol extract content is 57.4%; The content of lobetyolin is 0.85 mg/g in the Radix Codonopsis of use photosynthetic bacteria culture fluid, and alcohol extract content is 63.8%.Lobetyolin's content increases by 77.1%, and extract content increases by 8.2%.
Claims (2)
1. a photosynthetic bacteria culture fluid is used for the method that medicinal material is planted, and it is characterized in that comprising following plantation step:
In through the photosynthetic bacteria culture medium of sterilization, insert the photosynthetic bacteria seed culture fluid of 1/10-1/5, under the 2500Lux illumination condition, room temperature, anaerobism were cultivated 3-15 days, and obtaining viable count is 10
8-10
10The photosynthetic bacteria culture fluid of individual/ml;
The photosynthetic bacteria culture fluid is diluted with water to 10-100 times of original volume, and watering evenly to irrigate with the usage amount combination of every mu of 5-20kg imposes in the soil that will plant medicinal material;
The photosynthetic bacteria culture fluid is diluted with water to 10-100 times of original volume, and seed soaking is 10-60 minute before the sowing;
The 30-200 that the photosynthetic bacteria culture fluid is diluted with water to original volume doubly, final singling or transplant the back, go into the winter before, turned green in 1 year after, with the usage amount of every mu of medicinal material 1-10kg with the filling root that waters;
The 30-200 that the photosynthetic bacteria culture fluid is diluted with water to original volume doubly evenly sprays with the usage amount of every mu of each 1-10kg of medicinal material, medicinal material seedling stage 1 time, vegetative period 2 times then, or back 1 time, vegetative period 2 times of turning green in 1 year;
Photosynthetic bacteria is a Rhodospirillum in the described photosynthetic bacteria culture fluid
Rhodospirillium, Rhodopseudomonas
Rhodopseudomonas, red bacterium belongs to
RhodobacterIn any strain bacterium or their combination in any; Described Rhodospirillum
RhodospirilliumFor Rhodospirillum rubrum (
Rhodospirillium rubrum); Rhodopseudomonas
RhodopseudomonasFor Rhodopseudomonas palustris (
Rhodopseudomonas palustris), Green color rhodopseudomonas (
Rhodopseudomonas viridis), rhodopseudononas sulfovirdis (
Rhodopseudomonas sulfoviridis), red marine pseudomonas (
Rhodopseudomonas marina); Red bacterium belongs to
RhodobacterFor Spherical red antibacterial (
Rhodobacter sphaeroides).
2. the application of photosynthetic bacteria culture fluid in improving botanical active ingredient and extract content.
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