CN106106088A

CN106106088A - A kind of high infiltration rate Citrullus vulgaris culture matrix

Info

Publication number: CN106106088A
Application number: CN201610499563.9A
Authority: CN
Inventors: 温振海; 孙丽华; 肖彦波; 张毅
Original assignee: Yingquan District Of Fuyang City Ca Fruit And Vegetable Planting Cooperatives
Current assignee: Yingquan District Of Fuyang City Ca Fruit And Vegetable Planting Cooperatives
Priority date: 2016-06-28
Filing date: 2016-06-28
Publication date: 2016-11-16

Abstract

The invention discloses a kind of high infiltration rate Citrullus vulgaris culture matrix, be prepared by the raw materials in: Os Caprae seu Ovis powder 13 15, megasse 25 27, sodium molybdate 35, Borax 46, fulvic acid diamidogen ferrum 2.2 2.5, Sargassum powder 34, alkylphenol polyoxyethylene 1.3 1.6, sunflower plate granulated slag 35 37, Periostracum Serpentis waste material 42 45, pepsin preparation 1.3 1.6,1 (3 dimethylamino-propyl) 3 ethyl-carbodiimide hydrochloride 1.4 1.5, N N-Hydroxysuccinimide 0.4 0.5,4% sodium chloride solution are in right amount, 0.15mol/L citric acid solution is appropriate, water is appropriate；The culture matrix of the present invention is internal fluffy, the structure of alkylphenol polyoxyethylene collocation collagen protein can increase its content pore structure, increase moisture infiltration rate and the wettability of substrate, improve the horizontal wetted area of substrate, improve the water-retaining property of substrate and the utilization ratio to moisture, make to reduce irrigation quantity in Citrullus vulgaris substrate planting process, save Productive statistics and manual labor.

Description

A kind of high infiltration rate Citrullus vulgaris culture matrix

Technical field

The present invention relates to nutrient matrix technical field, particularly relate to a kind of high infiltration rate Citrullus vulgaris culture matrix.

Background technology

Substrate culture is a kind of important cultural method in the current agriculture of China, woods, flower planting industry, is that one passes through base Matter absorbs nutritional solution and a kind of Soilless Culture Methods of oxygen, owing to it is low to soil environment requirement, equipment simple, water-saving saves fertilizer etc. Advantage, is adopted application by vast plant development person.And continuous along with the development of China's plant husbandry and cultivated area Expanding, the usage amount of cultivation matrix the most constantly increases, and traditional uses the single raw material such as the peat composed of rotten mosses, Vermiculitum or be simply mixed preparation Substrate can not meet the demand of plantation, and the universal nutrition of present stage substrate simultaneously is single, causes sending out of plant in cultivation Bud rate is low, survival rate is low, the physical instability of substrate during simultaneously using, and water absorption is poor, easily subside, surcharge Low, it is impossible to realize higher using value for specific environment, affect the growth of plantation economy benefit.

Collagen protein, as a kind of biomacromolecule, has good pore structure after being modified, the most raw Thing degradability, mechanical strength and biocompatibility, have good cell growth interface, can become a kind of cultivation matrix and build In quality raw materials.China Serpentis aboundresources simultaneously, owing to the decorative pattern of Periostracum Serpentis outward appearance is the most gorgeous, pattern is unique, is used for making The artware of various finenesses, but due to the backwardness of Periostracum Serpentis Technology, the course of processing creates a large amount of waste material, account for complete The 20-30% of Periostracum Serpentis, if not being effectively treated, not only polluting environment, also wasting substantial amounts of raw material, containing substantial amounts of in Periostracum Serpentis Collagen protein, this also applies for it and provides cheap raw material basis in substrate preparation.

The application for a patent for invention of Publication No. CN1702061A discloses the full nutrient matrix of a kind of botanical seedling culturing and cultivation, This nutrient matrix use weight ratio be the peat of 50-70%, the perlite of 20-25%, the Vermiculitum of 10-15 and active substance, Nutrient substance mixes, and has more comprehensively nutrients structure, but the cultivation matrix of this disclosure of the invention is due to size not One, easily cause sedimentation after watering, cause the blocking of air in substrate, the crude fiber content in substrate is low simultaneously, needs extra battalion Support the interpolation of material, improve production cost, and not for the additional use value of the real needs raising substrate cultivated, limit Make the scale application of product.

Summary of the invention

The object of the invention is contemplated to make up the defect of prior art, it is provided that a kind of high infiltration rate Citrullus vulgaris culture matrix.

The present invention is achieved by the following technical solutions:

A kind of high infiltration rate Citrullus vulgaris culture matrix, is prepared by the raw materials in: Os Caprae seu Ovis powder 13-15, megasse 25- 27, sodium molybdate 3-5, Borax 4-6, fulvic acid diamidogen ferrum 2.2-2.5, Sargassum powder 3-4, alkylphenol polyoxyethylene 1.3-1.6, to Day certain herbaceous plants with big flowers dish granulated slag 35-37, Periostracum Serpentis waste material 42-45, pepsin preparation 1.3-1.6,1-(3-dimethylamino-propyl)-3-ethyl carbon Diimmonium salt hydrochlorate 1.4-1.5, N-hydroxy-succinamide 0.4-0.5,4% sodium chloride solution are appropriate, 0.15mol/L citric acid Solution is appropriate, water is appropriate.

Specifically comprising the following steps that of described high infiltration rate Citrullus vulgaris culture matrix preparation method

(1) Os Caprae seu Ovis powder, megasse mixing are placed in fermentation vat, add water to the 72-76% of ferment heap maximum water holding capacity, fermentation 24-28 days, by fermentation material squeeze and filter after having fermented, obtain fermentation liquid and ferment slag is standby, sunflower plate cut off simultaneously, pulverize Standby for smalls；

(2) 4% sodium chloride solution is added by 1:16-20g/mL solid-to-liquid ratio after being shredded by Periostracum Serpentis waste material, after soaking 6-8 hour Centrifugal 12-15 minute with 5800-6200 rev/min, take precipitation clear water clean and at 30-32 DEG C dry, then will precipitation with 0.15mol/L citric acid solution presses 1:8-10g/mL solid-to-liquid ratio mix homogeneously, adds pepsin preparation, is heated to 30-32 DEG C, Insulation is extracted 18-20 hour, completes post-heating and is warming up to 85-95 DEG C, is incubated 10 minutes, then is centrifuged with 5000-5500 rev/min 10-12 minute, collect the supernatant, obtain collagen solution standby；

(3) in step 1 gained fermentation liquid, add sodium molybdate, Borax, fulvic acid diamidogen ferrum, be heated to 52-56 DEG C, reaction After 28-32 minute cool down, then with step 1 gained ferment slag, sunflower plate granulated slag, Sargassum powder mixing for standby use；

(4) step 2 gained collagen solution and alkylphenol polyoxyethylene are joined in step 3 gained material, It is placed under 4-5 DEG C of low temperature environment, after stirring 5-10 minute, adds 1-(3-dimethylamino-propyl)-3-ethyl carbodiimide hydrochloride Salt, N-hydroxy-succinamide and other leftover materials, cross-linking reaction 8-10 hour, be warming up to 30-35 by material after completing DEG C dry, obtain substrate of the present invention.

The invention have the advantage that

Substrate of the present invention is by the extraction to Periostracum Serpentis, and by having reduced immunogenicity, the collagen protein of biodegradability should Use in the preparation of cultivation matrix, utilize the mechanical performance of cross-linking modified raising albumen, it is achieved its support in substrate builds Effect, can not only provide certain support and growth interface, the collagenous fiber bundle phase within collagen protein simultaneously for seed seedling Be staggered porous network structure mutually that formed, is also beneficial to beneficial microorganism and carries and the extension of root cell, and is It provides sufficient oxygen and nutrient substance, promotes the growth of plant, and culture matrix plays fixation, convenient transport.

The culture matrix of the present invention is internal fluffy, and the structure of alkylphenol polyoxyethylene collocation collagen protein can increase substrate Internal pore structure, increases moisture infiltration rate and the wettability of substrate, improves the horizontal wetted area of substrate, improve base The water-retaining property of matter and the utilization ratio to moisture so that reduce irrigation quantity in Citrullus vulgaris substrate planting process, save production and throw Enter and manual labor.

Detailed description of the invention

A kind of high infiltration rate Citrullus vulgaris culture matrix, is made up of the raw material of following weight portion (kg): Os Caprae seu Ovis powder 13, megasse 25, sodium molybdate 3, Borax 4, fulvic acid diamidogen ferrum 2.2, Sargassum powder 3, alkylphenol polyoxyethylene 1.3, sunflower plate granulated slag 35, Periostracum Serpentis waste material 42, pepsin preparation 1.3,1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride 1.4, N hydroxyl amber Amber acid imide 0.4,4% sodium chloride solution is appropriate, 0.15mol/L citric acid solution is appropriate, water is appropriate.

(1) Os Caprae seu Ovis powder, megasse mixing are placed in fermentation vat, add water to the 72% of ferment heap maximum water holding capacity, fermentation 24 My god, by fermentation material squeeze and filter after having fermented, obtain fermentation liquid and ferment slag is standby, sunflower plate cut off simultaneously, pulverize as carefully End is standby；

(2) add 4% sodium chloride solution by 1:16g/mL solid-to-liquid ratio after being shredded by Periostracum Serpentis waste material, after soaking 6 hours with 5800 revs/min are centrifuged 12 minutes, take precipitation clear water and clean and dry at 30 DEG C, then will precipitation and 0.15mol/L citric acid Solution presses 1:8g/mL solid-to-liquid ratio mix homogeneously, adds pepsin preparation, is heated to 30 DEG C, and insulation is extracted 18 hours, after completing It is heated to 85 DEG C, is incubated 10 minutes, then be centrifuged 10 minutes with 5000 revs/min, collect the supernatant, obtain collagen solution Standby；

(3) in step 1 gained fermentation liquid, add sodium molybdate, Borax, fulvic acid diamidogen ferrum, be heated to 52 DEG C, react 28 After minute cool down, then with step 1 gained ferment slag, sunflower plate granulated slag, Sargassum powder mixing for standby use；

(4) step 2 gained collagen solution and alkylphenol polyoxyethylene are joined in step 3 gained material, It is placed under 4 DEG C of low temperature environments, after stirring 5 minutes, adds 1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride, N hydroxyl Base butanimide and other leftover materials, cross-linking reaction 8 hours, after completing, material is warming up to 30 DEG C of drying, obtains this Invention substrate.

In order to further illustrate the using value of the present invention, inventor have chosen respectively substrate of the present invention and commercially available commonly Culture matrix carries out the cultivating and growing of Citrullus vulgaris, and during plantation, management method is identical, and during plantation, the physical and chemical index to substrate is carried out Detection, result is as follows:

	Full nitrogen (%)	Full phosphorus (%)	Full potassium (%)	Total porosity (%)	Saturation moisture content (%)
						Substrate of the present invention	0.901	0.11	0.44	89.84	886
Commercially available generic media	0.795	0.08	0.42	84.02	405

Substrate the most of the present invention plantation gained the yield of watermelon relatively generic media improves more than 10%, it can be seen that the present invention Substrate relatively generic media is the most sufficient, simultaneously stable in physicochemical property, and structure is excellent, has preferable prospect of the application.

Claims

1. one kind high infiltration rate Citrullus vulgaris culture matrix, it is characterised in that be prepared by the raw materials in: Os Caprae seu Ovis powder 13-15, Megasse 25-27, sodium molybdate 3-5, Borax 4-6, fulvic acid diamidogen ferrum 2.2-2.5, Sargassum powder 3-4, alkylphenol polyoxyethylene 1.3-1.6, sunflower plate granulated slag 35-37, Periostracum Serpentis waste material 42-45, pepsin preparation 1.3-1.6,1-(3-dimethylamino third Base)-3-ethyl-carbodiimide hydrochloride 1.4-1.5, N-hydroxy-succinamide 0.4-0.5,4% sodium chloride solution be appropriate, 0.15mol/L citric acid solution is appropriate, water is appropriate.

2. according to the high infiltration rate Citrullus vulgaris culture matrix described in claims 1, it is characterised in that the concrete steps of preparation method As follows:

(1) Os Caprae seu Ovis powder, megasse mixing being placed in fermentation vat, add water to the 72-76% of ferment heap maximum water holding capacity, ferment 24- 28 days, by fermentation material squeeze and filter after having fermented, obtain fermentation liquid and ferment slag is standby, sunflower plate cut off simultaneously, pulverize and be Smalls is standby；

(2) add 4% sodium chloride solution by 1:16-20g/mL solid-to-liquid ratio after being shredded by Periostracum Serpentis waste material, after soaking 6-8 hour with 5800-6200 rev/min is centrifugal 12-15 minute, take precipitation clear water clean and at 30-32 DEG C dry, then will precipitation with 0.15mol/L citric acid solution presses 1:8-10g/mL solid-to-liquid ratio mix homogeneously, adds pepsin preparation, is heated to 30-32 DEG C, Insulation is extracted 18-20 hour, completes post-heating and is warming up to 85-95 DEG C, is incubated 10 minutes, then is centrifuged with 5000-5500 rev/min 10-12 minute, collect the supernatant, obtain collagen solution standby；

(3) in step 1 gained fermentation liquid, add sodium molybdate, Borax, fulvic acid diamidogen ferrum, be heated to 52-56 DEG C, react 28- After 32 minutes cool down, then with step 1 gained ferment slag, sunflower plate granulated slag, Sargassum powder mixing for standby use；

(4) step 2 gained collagen solution and alkylphenol polyoxyethylene are joined in step 3 gained material, be placed in Under 4-5 DEG C of low temperature environment, after stirring 5-10 minute, add 1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride, N- N-Hydroxysuccinimide and other leftover materials, cross-linking reaction 8-10 hour, after completing, material is warming up to 30-35 DEG C of baking Dry, obtain substrate of the present invention.