CN106105865A - The cultivated strawberry substrate that the fermentation of a kind of dreg utilizes - Google Patents
The cultivated strawberry substrate that the fermentation of a kind of dreg utilizes Download PDFInfo
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- CN106105865A CN106105865A CN201610497433.1A CN201610497433A CN106105865A CN 106105865 A CN106105865 A CN 106105865A CN 201610497433 A CN201610497433 A CN 201610497433A CN 106105865 A CN106105865 A CN 106105865A
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- fermentation
- dreg
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Botany (AREA)
- Environmental & Geological Engineering (AREA)
- Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Environmental Sciences (AREA)
- Fertilizers (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses the cultivated strawberry substrate that the fermentation of a kind of dreg utilizes, it is prepared by the raw materials in: weathered coal 15 18, needle mushroom dreg 23 24, sodium bicarbonate 67, Diammonium phosphate (DAP) 46, aminotriacetic acid 12, crushed crude pearlite 34, antibiotic bacterium dregs 20 22, sunflower plate granulated slag 26 28, Periostracum Serpentis waste material 34 36, pepsin preparation 1.4 1.5, 1 (3 dimethylamino-propyl) 3 ethyl-carbodiimide hydrochloride 1.1 1.3, N N-Hydroxysuccinimide 0.4 0.5, 4% sodium chloride solution is appropriate, 0.15mol/L citric acid solution is appropriate, water is appropriate;The culture matrix of the present invention employs the industrial residues such as the most cheap antibiotic bacterium dregs, the most harmful garbage is converted into harmless high-quality organic material, and improve the utilization ratio of humus in substrate, growth for Fructus Fragariae Ananssae provides N P and K and various trace elements, it is achieved that the low-cost high-efficiency plantation of Fructus Fragariae Ananssae.
Description
Technical field
The present invention relates to nutrient matrix technical field, particularly relate to the cultivated strawberry substrate that the fermentation of a kind of dreg utilizes.
Background technology
Substrate culture is a kind of important cultural method in the current agriculture of China, woods, flower planting industry, is that one passes through base
Matter absorbs nutritional solution and a kind of Soilless Culture Methods of oxygen, owing to it is low to soil environment requirement, equipment simple, water-saving saves fertilizer etc.
Advantage, is adopted application by vast plant development person.And continuous along with the development of China's plant husbandry and cultivated area
Expanding, the usage amount of cultivation matrix the most constantly increases, and traditional uses the single raw material such as the peat composed of rotten mosses, Vermiculitum or be simply mixed preparation
Substrate can not meet the demand of plantation, and the universal nutrition of present stage substrate simultaneously is single, causes sending out of plant in cultivation
Bud rate is low, survival rate is low, the physical instability of substrate during simultaneously using, and water absorption is poor, easily subside, surcharge
Low, it is impossible to realize higher using value for specific environment, affect the growth of plantation economy benefit.
Collagen protein, as a kind of biomacromolecule, has good pore structure after being modified, the most raw
Thing degradability, mechanical strength and biocompatibility, have good cell growth interface, can become a kind of cultivation matrix and build
In quality raw materials.China Serpentis aboundresources simultaneously, owing to the decorative pattern of Periostracum Serpentis outward appearance is the most gorgeous, pattern is unique, is used for making
The artware of various finenesses, but due to the backwardness of Periostracum Serpentis Technology, the course of processing creates a large amount of waste material, account for complete
The 20-30% of Periostracum Serpentis, if not being effectively treated, not only polluting environment, also wasting substantial amounts of raw material, containing substantial amounts of in Periostracum Serpentis
Collagen protein, this also applies for it and provides cheap raw material basis in substrate preparation.
The application for a patent for invention of Publication No. CN1702061A discloses the full nutrient matrix of a kind of botanical seedling culturing and cultivation,
This nutrient matrix use weight ratio be the peat of 50-70%, the perlite of 20-25%, the Vermiculitum of 10-15 and active substance,
Nutrient substance mixes, and has more comprehensively nutrients structure, but the cultivation matrix of this disclosure of the invention is due to size not
One, easily cause sedimentation after watering, cause the blocking of air in substrate, the crude fiber content in substrate is low simultaneously, needs extra battalion
Support the interpolation of material, improve production cost, and not for the additional use value of the real needs raising substrate cultivated, limit
Make the scale application of product.
Summary of the invention
The object of the invention is contemplated to make up the defect of prior art, it is provided that the cultivated strawberry base that the fermentation of a kind of dreg utilizes
Matter.
The present invention is achieved by the following technical solutions:
The cultivated strawberry substrate that the fermentation of a kind of dreg utilizes, is prepared by the raw materials in: weathered coal 15-18, gold
Pin mushroom slag 23-24, sodium bicarbonate 6-7, Diammonium phosphate (DAP) 4-6, aminotriacetic acid 1-2, crushed crude pearlite 3-4, antibiotic bacterium dregs 20-
22, sunflower plate granulated slag 26-28, Periostracum Serpentis waste material 34-36, pepsin preparation 1.4-1.5,1-(3-dimethylamino-propyl)-3-
Ethyl-carbodiimide hydrochloride 1.1-1.3, N-hydroxy-succinamide 0.4-0.5,4% sodium chloride solution are appropriate, 0.15mol/L
Citric acid solution is appropriate, water is appropriate.
Specifically comprising the following steps that of the cultivated strawberry Matrix formulation procedure that described dreg fermentation utilizes
(1) weathered coal, needle mushroom dreg, antibiotic bacterium dregs mixing are placed in fermentation vat, add water to ferment heap maximum aqueous
The 73-76% of amount, in fermentation 2-3 week, by fermentation material squeeze and filter after having fermented, obtains fermentation liquid and ferment slag is standby, simultaneously will be to
Day certain herbaceous plants with big flowers dish cuts off, it is standby to pulverize as smalls;
(2) 4% sodium chloride solution is added by 1:16-20g/mL solid-to-liquid ratio after being shredded by Periostracum Serpentis waste material, after soaking 6-8 hour
Centrifugal 12-15 minute with 5800-6200 rev/min, take precipitation clear water clean and at 30-32 DEG C dry, then will precipitation with
0.15mol/L citric acid solution presses 1:8-10g/mL solid-to-liquid ratio mix homogeneously, adds pepsin preparation, is heated to 30-32 DEG C,
Insulation is extracted 20-24 hour, completes post-heating and is warming up to 85-95 DEG C, is incubated 10 minutes, then is centrifuged with 5000-5500 rev/min
10-12 minute, collect the supernatant, obtain collagen solution standby;
(3) in step 1 gained fermentation liquid, add sodium bicarbonate, Diammonium phosphate (DAP), aminotriacetic acid, be heated to 58-62 DEG C,
After reacting 25-30 minute cool down, then with step 1 gained ferment slag, sunflower plate granulated slag, crushed crude pearlite mixing for standby use;
(4) step 2 gained collagen solution is joined in step 3 gained material, is placed under 4-5 DEG C of low temperature environment,
After stirring 5-10 minute add 1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride, N-hydroxy-succinamide with
And other leftover materials, cross-linking reaction 8-10 hour, after completing, material is warming up to 30-35 DEG C of drying, obtains substrate of the present invention.
The invention have the advantage that
Substrate of the present invention is by the extraction to Periostracum Serpentis, and by having reduced immunogenicity, the collagen protein of biodegradability should
Use in the preparation of cultivation matrix, utilize the mechanical performance of cross-linking modified raising albumen, it is achieved its support in substrate builds
Effect, can not only provide certain support and growth interface, the collagenous fiber bundle phase within collagen protein simultaneously for seed seedling
Be staggered porous network structure mutually that formed, is also beneficial to beneficial microorganism and carries and the extension of root cell, and is
It provides sufficient oxygen and nutrient substance, promotes the growth of plant, and culture matrix plays fixation, convenient transport.
The culture matrix of the present invention employs the industrial residues such as the most cheap antibiotic bacterium dregs, the most harmful is discarded
Thing is converted into harmless high-quality organic material, and improves the utilization ratio of humus in substrate, and the growth for Fructus Fragariae Ananssae provides nitrogen
Phosphorus potassium and various trace elements, it is achieved that the low-cost high-efficiency plantation of Fructus Fragariae Ananssae.
Detailed description of the invention
The cultivated strawberry substrate that the fermentation of a kind of dreg utilizes, is made up of the raw material of following weight portion (kg): weathered coal 15, gold
Pin mushroom slag 23, sodium bicarbonate 6, Diammonium phosphate (DAP) 4, aminotriacetic acid 1, crushed crude pearlite 3, antibiotic bacterium dregs 20, sunflower plate powder
Slag 26, Periostracum Serpentis waste material 34, pepsin preparation 1.4,1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride 1.1, N
N-Hydroxysuccinimide 0.4,4% sodium chloride solution is appropriate, 0.15mol/L citric acid solution is appropriate, water is appropriate.
Specifically comprising the following steps that of the cultivated strawberry Matrix formulation procedure that described dreg fermentation utilizes
(1) weathered coal, needle mushroom dreg, antibiotic bacterium dregs mixing are placed in fermentation vat, add water to ferment heap maximum aqueous
The 73% of amount, ferments 2 weeks, by fermentation material squeeze and filter after having fermented, obtains fermentation liquid and ferment slag is standby, simultaneously by sunflower plate
Cut off, pulverize as smalls standby;
(2) add 4% sodium chloride solution by 1:16g/mL solid-to-liquid ratio after being shredded by Periostracum Serpentis waste material, after soaking 6 hours with
5800 revs/min are centrifuged 12 minutes, take precipitation clear water and clean and dry at 30 DEG C, then will precipitation and 0.15mol/L citric acid
Solution presses 1:8g/mL solid-to-liquid ratio mix homogeneously, adds pepsin preparation, is heated to 30 DEG C, and insulation is extracted 20 hours, after completing
It is heated to 85 DEG C, is incubated 10 minutes, then be centrifuged 10 minutes with 5000 revs/min, collect the supernatant, obtain collagen solution
Standby;
(3) in step 1 gained fermentation liquid, add sodium bicarbonate, Diammonium phosphate (DAP), aminotriacetic acid, be heated to 58 DEG C, instead
After answering 25 minutes cool down, then with step 1 gained ferment slag, sunflower plate granulated slag, crushed crude pearlite mixing for standby use;
(4) step 2 gained collagen solution is joined in step 3 gained material, be placed under 4 DEG C of low temperature environments, stir
After mixing 5 minutes add 1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride, N N-Hydroxysuccinimide and other
Leftover materials, cross-linking reaction 8 hours, after completing, material is warming up to 30 DEG C of drying, obtains substrate of the present invention.
In order to further illustrate the using value of the present invention, inventor have chosen respectively substrate of the present invention and commercially available commonly
Culture matrix carries out the cultivating and growing of Fructus Fragariae Ananssae, and during plantation, management method is identical, and during plantation, the physical and chemical index to substrate is carried out
Detection, result is as follows:
Full nitrogen (%) | Full phosphorus (%) | Full potassium (%) | Total porosity (%) | Saturation moisture content (%) | |
Substrate of the present invention | 0.923 | 0.11 | 0.43 | 89.05 | 890 |
Commercially available generic media | 0.783 | 0.08 | 0.38 | 84.56 | 457 |
Substrate the most of the present invention plantation gained Yield of Strawberry relatively generic media improves more than 10%, it can be seen that the present invention
Substrate relatively generic media is the most sufficient, simultaneously stable in physicochemical property, and structure is excellent, has preferable prospect of the application.
Claims (2)
1. the cultivated strawberry substrate that a dreg fermentation utilizes, it is characterised in that be prepared by the raw materials in: weathered coal
15-18, needle mushroom dreg 23-24, sodium bicarbonate 6-7, Diammonium phosphate (DAP) 4-6, aminotriacetic acid 1-2, crushed crude pearlite 3-4, antibiosis
Element dreg 20-22, sunflower plate granulated slag 26-28, Periostracum Serpentis waste material 34-36, pepsin preparation 1.4-1.5,1-(3-dimethylamino
Propyl group)-3-ethyl-carbodiimide hydrochloride 1.1-1.3, N-hydroxy-succinamide 0.4-0.5,4% sodium chloride solution be appropriate,
0.15mol/L citric acid solution is appropriate, water is appropriate.
2. the cultivated strawberry substrate utilized according to the dreg fermentation described in claims 1, it is characterised in that the tool of preparation method
Body step is as follows:
(1) weathered coal, needle mushroom dreg, antibiotic bacterium dregs mixing are placed in fermentation vat, add water to ferment heap maximum water holding capacity
73-76%, in fermentation 2-3 week, by fermentation material squeeze and filter after having fermented, obtains fermentation liquid and ferment slag is standby, simultaneously by sunflower plate
Cut off, pulverize as smalls standby;
(2) add 4% sodium chloride solution by 1:16-20g/mL solid-to-liquid ratio after being shredded by Periostracum Serpentis waste material, after soaking 6-8 hour with
5800-6200 rev/min is centrifugal 12-15 minute, take precipitation clear water clean and at 30-32 DEG C dry, then will precipitation with
0.15mol/L citric acid solution presses 1:8-10g/mL solid-to-liquid ratio mix homogeneously, adds pepsin preparation, is heated to 30-32 DEG C,
Insulation is extracted 20-24 hour, completes post-heating and is warming up to 85-95 DEG C, is incubated 10 minutes, then is centrifuged with 5000-5500 rev/min
10-12 minute, collect the supernatant, obtain collagen solution standby;
(3) in step 1 gained fermentation liquid, add sodium bicarbonate, Diammonium phosphate (DAP), aminotriacetic acid, be heated to 58-62 DEG C, instead
After answering 25-30 minute cool down, then with step 1 gained ferment slag, sunflower plate granulated slag, crushed crude pearlite mixing for standby use;
(4) step 2 gained collagen solution is joined in step 3 gained material, be placed under 4-5 DEG C of low temperature environment, stirring
After 5-10 minute add 1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride, N-hydroxy-succinamide and its
His leftover materials, cross-linking reaction 8-10 hour, after completing, material is warming up to 30-35 DEG C of drying, obtains substrate of the present invention.
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107853129A (en) * | 2017-11-24 | 2018-03-30 | 浙江省农业科学院 | A kind of strawberry cultivation substrate |
CN109156315A (en) * | 2018-09-21 | 2019-01-08 | 卫卓然 | A kind of fleshiness culture medium preparation method |
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CN103073343A (en) * | 2013-01-08 | 2013-05-01 | 李庆康 | Low-carbon, environment-friendly and efficient organic vegetable culture medium and preparation method thereof |
CN103224433A (en) * | 2013-05-16 | 2013-07-31 | 山东省农业科学院农业资源与环境研究所 | Soil fertility improver produced by utilizing waste bacterial residues |
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CN103627761A (en) * | 2013-10-22 | 2014-03-12 | 浙江省海洋开发研究院 | Method for preparing collagen peptide rich in hydroxyproline |
CN103975837A (en) * | 2014-06-08 | 2014-08-13 | 云南睿智农业科技有限公司 | Soilless culture substrate for vegetables |
CN104447092A (en) * | 2014-12-03 | 2015-03-25 | 大新县生产力促进中心 | Special composite fertilizer for broadleaf holly leaves and production method of composite fertilizer |
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2016
- 2016-06-28 CN CN201610497433.1A patent/CN106105865A/en not_active Withdrawn
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
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CN102093094A (en) * | 2009-12-14 | 2011-06-15 | 刘继军 | Biological bacteria fertilizer and method for producing organic fertilizer by utilizing agricultural solid organic waste |
CN103073343A (en) * | 2013-01-08 | 2013-05-01 | 李庆康 | Low-carbon, environment-friendly and efficient organic vegetable culture medium and preparation method thereof |
CN103224433A (en) * | 2013-05-16 | 2013-07-31 | 山东省农业科学院农业资源与环境研究所 | Soil fertility improver produced by utilizing waste bacterial residues |
CN103238506A (en) * | 2013-05-23 | 2013-08-14 | 山东商道生物科技有限公司 | Multiple cropping regeneration cultivation method for vegetables by utilizing all organic substrates |
CN103627761A (en) * | 2013-10-22 | 2014-03-12 | 浙江省海洋开发研究院 | Method for preparing collagen peptide rich in hydroxyproline |
CN103975837A (en) * | 2014-06-08 | 2014-08-13 | 云南睿智农业科技有限公司 | Soilless culture substrate for vegetables |
CN104447092A (en) * | 2014-12-03 | 2015-03-25 | 大新县生产力促进中心 | Special composite fertilizer for broadleaf holly leaves and production method of composite fertilizer |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107853129A (en) * | 2017-11-24 | 2018-03-30 | 浙江省农业科学院 | A kind of strawberry cultivation substrate |
CN109156315A (en) * | 2018-09-21 | 2019-01-08 | 卫卓然 | A kind of fleshiness culture medium preparation method |
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