CN106069021A - A kind of method utilizing Fructus Trichosanthis tuber quickly breeding high-quality seedling - Google Patents

A kind of method utilizing Fructus Trichosanthis tuber quickly breeding high-quality seedling Download PDF

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CN106069021A
CN106069021A CN201610430323.3A CN201610430323A CN106069021A CN 106069021 A CN106069021 A CN 106069021A CN 201610430323 A CN201610430323 A CN 201610430323A CN 106069021 A CN106069021 A CN 106069021A
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tuber
parts
fructus trichosanthis
water
vitamin
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吴权明
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ZONGYANG COUNTY BAIYUN ECOLOGICAL GARDEN Co Ltd
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ZONGYANG COUNTY BAIYUN ECOLOGICAL GARDEN Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/10Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/14Boron; Compounds thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/26Phosphorus; Compounds thereof
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B7/00Fertilisers based essentially on alkali or ammonium orthophosphates
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05CNITROGENOUS FERTILISERS
    • C05C5/00Fertilisers containing other nitrates
    • C05C5/04Fertilisers containing other nitrates containing calcium nitrate
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/20Liquid fertilisers
    • C05G5/23Solutions

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  • Life Sciences & Earth Sciences (AREA)
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  • Pest Control & Pesticides (AREA)
  • Chemical & Material Sciences (AREA)
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  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Inorganic Chemistry (AREA)
  • Botany (AREA)
  • Cultivation Of Plants (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a kind of method utilizing Fructus Trichosanthis tuber quickly breeding high-quality seedling, comprise the steps: that (1) tuber is chosen, prepared by (2) culture medium, prepared by (3) root treatment fluid, prepared by (4) nutritional solution, the process of (5) tuber, (6) tuber heeling-in, manage after (7) heeling-in.The inventive method can be bred as finished product Fructus Trichosanthis seedling for 27 ~ 29 days, shortens about 10 days than traditional method, and the incubation rate of tuber reaches more than 90%, the finished product Fructus Trichosanthis seedling replanting survival rate more than 95% after bringing out, and has good economic worth.

Description

A kind of method utilizing Fructus Trichosanthis tuber quickly breeding high-quality seedling
Technical field
The invention belongs to field of planting, be specifically related to a kind of method utilizing Fructus Trichosanthis tuber quickly breeding high-quality seedling.
Background technology
Fructus Trichosanthis, the perennial climbing herb of Cucurbitaceae snake gourd, long up to 10 meters.Root stock is plump, cylindric, outer Beijing opera Color.Stem multi-branched, without hair;Leaf alternate, subcircular or heart, dioecism;Male flower number piece raceme, rare Dan Sheng, corolla Sliver obovate, female flower Dan Sheng, ovary is avette, and fruit is subsphaeroidal, orange red time ripe, 7 ~ November of flowering fruit bearing stage.
Fructus Trichosanthis have antipyretic quench the thirst, diuresis, the effect such as relieving cough and expelling phlegm.Seed is containing fat flower fat oil;Fruit is containing triterpene saponin, organic Acid, resin, saccharide, pigment;Root contains protein, saponin, acids.Fructus Trichosanthis rich in nutritive value, rich in nutritional labeling, wherein albumen Matter, fat, cellulose, trace element and alkaloid, flavonoid, infantile malnutrition class and organic equal size are rich.Fructus Trichosanthis breeds children at present The mode of Seedling has: seminal propagation, tuber breeding and breeding of keeping down the vines of a creeping plant, the problem that character changes easily occurs in seminal propagation, breeding of keeping down the vines of a creeping plant Operation more bother, and incubation rate is the highest, and tuber breeding is the ideal mating system that can continue Fructus Trichosanthis merit, But the seedling that existing tuber propagation method also exists more, and seedling raise period is longer, bad root phenomenon easily occurs, brings out in cultivating process The problems such as survival rate is relatively low.The Fructus Trichosanthis tuber method for culturing seedlings of a kind of high-quality is needed badly on market.
Summary of the invention
In order to solve the problems referred to above, the invention provides a kind of side utilizing Fructus Trichosanthis tuber quickly breeding high-quality seedling Method.
The present invention is achieved through the following technical solutions:
A kind of method utilizing Fructus Trichosanthis tuber quickly breeding high-quality seedling, comprises the steps:
(1) tuber is chosen: with result 3 ~ 4 years, robust growth, without the breeding Fructus Trichosanthis of pest and disease damage for choosing object, in the Ching Ming Festival before After dig out its tuber, select the thick 2 ~ 4cm in footpath, section white, fresh tuber without pest and disease damage, the segment being cut into 4 ~ 6cm length is standby;
(2) prepared by culture medium:
Weigh 10 parts of turfy soils, 10 parts of celestial soil, 5 parts of Kaolin, 5 portions of bagasse and 2 parts of expanded perlites the most by weight;
The most first bagasse is put into immersion 1.5h in the daucic acid solution that mass fraction is 4%, after taking-up, uses deionized water rinsing Totally, then it is thermally dried by the temperature of 60 ~ 65 DEG C, when the most overall water content is 40 ~ 45%, places into air blasting machine In carry out air blasting process, air blasting places into after processing in the mixed liquor of its quality 2 times soaks 20min, and period does not stop stirring, finally Being thermally dried, by the temperature of 50 ~ 55 DEG C, the mixed liquor filling bagasse, the water content to bagasse is not more than 10%, Modified bagasse is standby;Described mixed liquor is made up of the material of following weight portion: 2 parts of carbamide, 1 part of potassium fulvate, 0.5 part of mistake phosphorus Acid calcium, 0.5 part of ferrous sulfate, 0.3 part of manganese sulfate, 0.1 part of copper sulfate, 0.3 part of zinc molybdate, 0.2 part of Bamboo vinegar solution, 110 parts of water;Right It is modified that bagasse carries out aforesaid way, can improve the absorption property of bagasse, reduces the turnover rate of nutrient substance, can adsorb again The harmful substances such as some heavy metals, for the mitogenetic growing environment providing safety and Health of tuber;
C. after turfy soil, celestial soil, Kaolin, the modified bagasse of step b gained and expanded perlite fully being puddled uniformly i.e. Obtain culture medium;The culture medium of preparation has good permeability, is suitable for the cultivation of Fructus Trichosanthis tuber, significantly reduces and cultivated In journey, the generation of bad root phenomenon, improves incubation rate;
(3) prepared by root treatment fluid:
A. take appropriate naphthalene acetic acid and dissolve in water, be made into the naphthalene acetic acid aqueous solution that concentration is 30 ~ 50mg/L;
B. in naphthalene acetic acid aqueous solution, add the sucrose of its gross mass 2%, 0.5% lycium barbarum polysaccharide, 1% water-soluble chitosan, 1% boric acid With 0.5% potassium dihydrogen phosphate, then heating keeps solution temperature to be 40 ~ 45 DEG C, and stirs all with the rotating speed of 200 ~ 300 revs/min Even;
(4) prepared by nutritional solution:
Weigh the most by weight 12 parts of flower of Caulis wisteriae sinensis, 10 portions of Thallus Laminariae (Thallus Eckloniae)s, 6 parts of lemon grass (Cymbopogon citratus)s, 2 parts of compound vitamines, 1 part of aminoacids complex, 1 Part hard magnesium, 0.5 part of sodium selenite, 1 part of four water-calcium nitrate;
B. flower of Caulis wisteriae sinensis, Thallus Laminariae (Thallus Eckloniae) and lemon grass (Cymbopogon citratus) being put in the clear water of its gross mass 3 times, boiling water decocts 15min, and period is simultaneously with super Sound wave carries out assisted extraction, is first ultrasonic extraction 5min of 40KHz by frequency, and reuse frequency is the ultrasonic extraction of 43KHz 5min, last reuse frequency is ultrasonic extraction 5min of 46KHz, filters to get filtrate standby;Enter with the ultrasound wave of different frequency range Row extracts successively, can improve kind and the quantity of beneficiating ingredient, coordinate the extraction of boiling water high temperature simultaneously, improve filtrate further Use value, shorten again extraction duration;
C. compound vitamin, aminoacids complex, hard magnesium, sodium selenite and four water-calcium nitrate are put in filtrate jointly, no Break and be stirred until homogeneous;The nutritional solution of preparation is balanced in nutrition comprehensively, meets the nutritional need rule that tuber is mitogenetic, improves children The growth situation that Seedling is overall, with root treatment fluid with the use of, can be under less Plant Hormone Action, moreover it is possible to preferably, relatively The fast growth promoting seedling root;
(5) tuber processes:
A. in the centre of tuber, cut off a upper-lower height be 4 ~ 6mm, breach around tuber 1/4 ~ 1/3 circumference, then split at this Cover flap sponge on mouth, and be fixed on tuber with rubber band;The breach height offered on tuber can not be excessive, otherwise Can affect the normal development of seedling, too small then DeGrain, the breach of aforesaid way can be the most directly the growth of seedling Supplement nutrient, promote its growth promoter;
B., the two ends otch of tuber is respectively put in root treatment fluid immersion 10min, and period heating keeps root treatment fluid Temperature is 40 ~ 45 DEG C, takes out, spread plant ash more standby on the otch at two ends after completing;
(6) tuber heeling-in: the tuber level after processing is buried and is placed in culture medium, will be covered with the breach of sponge and faces up and put Putting, the degree of depth of heeling-in is 5 ~ 7cm, and draws the liquid-transport pipe-line of an a diameter of 3mm from sponge, and the other end is drawn culture medium and used In transfusion, 15 ~ 20 tuber of every square metre of heeling-in;
(7) manage after heeling-in: after tuber heeling-in, pass through liquid-transport pipe-line sponge input nutritional solution on tuber breach every day, often They three times, the input quantity of each each tuber is 5 ~ 7g, inputs continuously 15 ~ 20 days, after seedling is bred as, is moved out and removes Rubber band and sponge.
Further, temperature when step (2) described air blasting processes is 280 DEG C, pressure is 11MPa.
Further, containing vitamin A, vitamin B1, vitamin B6, vitamin C in step (4) described compound vitamin And vitamin E, containing methionine, lysine, glycine, glutamic acid, proline and phenylalanine in described aminoacids complex.
There is advantages that tuber, culture medium, root treatment fluid, nutritional solution etc. are cultivated by the present invention During key step composition carried out special rational process, by the comprehensive applying of root treatment fluid and nutritional solution, carry The speed of emergence of high tuber and quality of emerging, improving traditional single applying root treatment fluid, to easily cause seedling quality irregular not Together, the phenomenon that subsequent growth is weak;Applying nutritional solution at the breach of tuber central authorities, use the most direct, its action effect is more Good, the utilization rate of nutritional solution material composition can not only be improved, can guarantee that again the development quality of seedling entirety and follow-up growing way;? Under the comprehensive function of each step, the inventive method can be bred as finished product Fructus Trichosanthis seedling for 27 ~ 29 days, shortens 10 days than traditional method Left and right, and the incubation rate of tuber reaches more than 90%, the finished product Fructus Trichosanthis seedling replanting survival rate more than 95% after bringing out, and has well Economic worth.
Detailed description of the invention
Embodiment 1
A kind of method utilizing Fructus Trichosanthis tuber quickly breeding high-quality seedling, comprises the steps:
(1) tuber is chosen: with result 3 ~ 4 years, robust growth, without the breeding Fructus Trichosanthis of pest and disease damage for choosing object, in the Ching Ming Festival before After dig out its tuber, select the thick 2 ~ 4cm in footpath, section white, fresh tuber without pest and disease damage, the segment being cut into 4 ~ 6cm length is standby;
(2) prepared by culture medium:
Weigh 10 parts of turfy soils, 10 parts of celestial soil, 5 parts of Kaolin, 5 portions of bagasse and 2 parts of expanded perlites the most by weight;
The most first bagasse is put into immersion 1.5h in the daucic acid solution that mass fraction is 4%, after taking-up, uses deionized water rinsing Totally, then it is thermally dried by the temperature of 60 ~ 65 DEG C, when the most overall water content is 40 ~ 45%, places into air blasting machine In carry out air blasting process, air blasting places into after processing in the mixed liquor of its quality 2 times soaks 20min, and period does not stop stirring, finally Being thermally dried, by the temperature of 50 ~ 55 DEG C, the mixed liquor filling bagasse, the water content to bagasse is not more than 10%, Modified bagasse is standby;Described mixed liquor is made up of the material of following weight portion: 2 parts of carbamide, 1 part of potassium fulvate, 0.5 part of mistake phosphorus Acid calcium, 0.5 part of ferrous sulfate, 0.3 part of manganese sulfate, 0.1 part of copper sulfate, 0.3 part of zinc molybdate, 0.2 part of Bamboo vinegar solution, 110 parts of water;
C. after turfy soil, celestial soil, Kaolin, the modified bagasse of step b gained and expanded perlite fully being puddled uniformly i.e. Obtain culture medium;
(3) prepared by root treatment fluid:
A. take appropriate naphthalene acetic acid and dissolve in water, be made into the naphthalene acetic acid aqueous solution that concentration is 30 ~ 50mg/L;
B. in naphthalene acetic acid aqueous solution, add the sucrose of its gross mass 2%, 0.5% lycium barbarum polysaccharide, 1% water-soluble chitosan, 1% boric acid With 0.5% potassium dihydrogen phosphate, then heating keeps solution temperature to be 40 ~ 45 DEG C, and stirs all with the rotating speed of 200 ~ 300 revs/min Even;
(4) prepared by nutritional solution:
Weigh the most by weight 12 parts of flower of Caulis wisteriae sinensis, 10 portions of Thallus Laminariae (Thallus Eckloniae)s, 6 parts of lemon grass (Cymbopogon citratus)s, 2 parts of compound vitamines, 1 part of aminoacids complex, 1 Part hard magnesium, 0.5 part of sodium selenite, 1 part of four water-calcium nitrate;
B. flower of Caulis wisteriae sinensis, Thallus Laminariae (Thallus Eckloniae) and lemon grass (Cymbopogon citratus) being put in the clear water of its gross mass 3 times, boiling water decocts 15min, and period is simultaneously with super Sound wave carries out assisted extraction, is first ultrasonic extraction 5min of 40KHz by frequency, and reuse frequency is the ultrasonic extraction of 43KHz 5min, last reuse frequency is ultrasonic extraction 5min of 46KHz, filters to get filtrate standby;
C. compound vitamin, aminoacids complex, hard magnesium, sodium selenite and four water-calcium nitrate are put in filtrate jointly, no Break and be stirred until homogeneous;
(5) tuber processes:
A. in the centre of tuber, cut off a upper-lower height be 4 ~ 6mm, breach around tuber 1/4 ~ 1/3 circumference, then split at this Cover flap sponge on mouth, and be fixed on tuber with rubber band;
B., the two ends otch of tuber is respectively put in root treatment fluid immersion 10min, and period heating keeps root treatment fluid Temperature is 40 ~ 45 DEG C, takes out, spread plant ash more standby on the otch at two ends after completing;
(6) tuber heeling-in: the tuber level after processing is buried and is placed in culture medium, will be covered with the breach of sponge and faces up and put Putting, the degree of depth of heeling-in is 5 ~ 7cm, and draws the liquid-transport pipe-line of an a diameter of 3mm from sponge, and the other end is drawn culture medium and used In transfusion, 15 ~ 20 tuber of every square metre of heeling-in;
(7) manage after heeling-in: after tuber heeling-in, pass through liquid-transport pipe-line sponge input nutritional solution on tuber breach every day, often They three times, the input quantity of each each tuber is 5 ~ 7g, inputs continuously 15 ~ 20 days, after seedling is bred as, is moved out and removes Rubber band and sponge.
Further, temperature when step (2) described air blasting processes is 280 DEG C, pressure is 11MPa.
Further, containing vitamin A, vitamin B1, vitamin B6, vitamin C in step (4) described compound vitamin And vitamin E, containing methionine, lysine, glycine, glutamic acid, proline and phenylalanine in described aminoacids complex.
Comparative example 1
This comparative example 1 compared with Example 1, does not carry out cracking treatment to tuber central authorities, and the mode applying nutritional solution is Being applied directly in the culture medium around tuber, method step in addition is identical.
Matched group
Existing Fructus Trichosanthis tuber method for culturing seedlings.
In order to contrast effect of the present invention, select " No. 3 " kind Fructus Trichosanthis, " No. 8 " kind Fructus Trichosanthis, " No. 9 " kind Fructus Trichosanthis conduct Experimental subject, in order to simplify expression, is indicated the Fructus Trichosanthis of above-mentioned three kinds of kinds with a, b, c, respectively then to a, b, c tri-kinds The Fructus Trichosanthis of kind is cultivated by above-mentioned three kinds of methods respectively, and following table is corresponding correction data:
Note: the incubation rate described in upper table refers to that the Fructus Trichosanthis seedling total amount being bred as accounts for the ratio of tuber total amount;Described transplant survival Rate refers to the survival rate after the Fructus Trichosanthis seedling replanting field planting being bred as.

Claims (3)

1. the method utilizing Fructus Trichosanthis tuber quickly breeding high-quality seedling, it is characterised in that comprise the steps:
(1) tuber is chosen: with result 3 ~ 4 years, robust growth, without the breeding Fructus Trichosanthis of pest and disease damage for choosing object, in the Ching Ming Festival before After dig out its tuber, select the thick 2 ~ 4cm in footpath, section white, fresh tuber without pest and disease damage, the segment being cut into 4 ~ 6cm length is standby;
(2) prepared by culture medium:
Weigh 10 parts of turfy soils, 10 parts of celestial soil, 5 parts of Kaolin, 5 portions of bagasse and 2 parts of expanded perlites the most by weight;
The most first bagasse is put into immersion 1.5h in the daucic acid solution that mass fraction is 4%, after taking-up, uses deionized water rinsing Totally, then it is thermally dried by the temperature of 60 ~ 65 DEG C, when the most overall water content is 40 ~ 45%, places into air blasting machine In carry out air blasting process, air blasting places into after processing in the mixed liquor of its quality 2 times soaks 20min, and period does not stop stirring, finally Being thermally dried, by the temperature of 50 ~ 55 DEG C, the mixed liquor filling bagasse, the water content to bagasse is not more than 10%, Modified bagasse is standby;Described mixed liquor is made up of the material of following weight portion: 2 parts of carbamide, 1 part of potassium fulvate, 0.5 part of mistake phosphorus Acid calcium, 0.5 part of ferrous sulfate, 0.3 part of manganese sulfate, 0.1 part of copper sulfate, 0.3 part of zinc molybdate, 0.2 part of Bamboo vinegar solution, 110 parts of water;
C. after turfy soil, celestial soil, Kaolin, the modified bagasse of step b gained and expanded perlite fully being puddled uniformly i.e. Obtain culture medium;
(3) prepared by root treatment fluid:
A. take appropriate naphthalene acetic acid and dissolve in water, be made into the naphthalene acetic acid aqueous solution that concentration is 30 ~ 50mg/L;
B. in naphthalene acetic acid aqueous solution, add the sucrose of its gross mass 2%, 0.5% lycium barbarum polysaccharide, 1% water-soluble chitosan, 1% boric acid With 0.5% potassium dihydrogen phosphate, then heating keeps solution temperature to be 40 ~ 45 DEG C, and stirs all with the rotating speed of 200 ~ 300 revs/min Even;
(4) prepared by nutritional solution:
Weigh the most by weight 12 parts of flower of Caulis wisteriae sinensis, 10 portions of Thallus Laminariae (Thallus Eckloniae)s, 6 parts of lemon grass (Cymbopogon citratus)s, 2 parts of compound vitamines, 1 part of aminoacids complex, 1 Part hard magnesium, 0.5 part of sodium selenite, 1 part of four water-calcium nitrate;
B. flower of Caulis wisteriae sinensis, Thallus Laminariae (Thallus Eckloniae) and lemon grass (Cymbopogon citratus) being put in the clear water of its gross mass 3 times, boiling water decocts 15min, and period is simultaneously with super Sound wave carries out assisted extraction, is first ultrasonic extraction 5min of 40KHz by frequency, and reuse frequency is the ultrasonic extraction of 43KHz 5min, last reuse frequency is ultrasonic extraction 5min of 46KHz, filters to get filtrate standby;
C. compound vitamin, aminoacids complex, hard magnesium, sodium selenite and four water-calcium nitrate are put in filtrate jointly, no Break and be stirred until homogeneous;
(5) tuber processes:
A. in the centre of tuber, cut off a upper-lower height be 4 ~ 6mm, breach around tuber 1/4 ~ 1/3 circumference, then split at this Cover flap sponge on mouth, and be fixed on tuber with rubber band;
B., the two ends otch of tuber is respectively put in root treatment fluid immersion 10min, and period heating keeps root treatment fluid Temperature is 40 ~ 45 DEG C, takes out, spread plant ash more standby on the otch at two ends after completing;
(6) tuber heeling-in: the tuber level after processing is buried and is placed in culture medium, will be covered with the breach of sponge and faces up and put Putting, the degree of depth of heeling-in is 5 ~ 7cm, and draws the liquid-transport pipe-line of an a diameter of 3mm from sponge, and the other end is drawn culture medium and used In transfusion, 15 ~ 20 tuber of every square metre of heeling-in;
(7) manage after heeling-in: after tuber heeling-in, pass through liquid-transport pipe-line sponge input nutritional solution on tuber breach every day, often They three times, the input quantity of each each tuber is 5 ~ 7g, inputs continuously 15 ~ 20 days, after seedling is bred as, is moved out and removes Rubber band and sponge.
A kind of method utilizing Fructus Trichosanthis tuber quickly breeding high-quality seedling the most according to claim 1, it is characterised in that Temperature when step (2) described air blasting processes is 280 DEG C, pressure is 11MPa.
A kind of method utilizing Fructus Trichosanthis tuber quickly breeding high-quality seedling the most according to claim 1, it is characterised in that Containing vitamin A, vitamin B1, vitamin B6, vitamin C and vitamin E in step (4) described compound vitamin, described multiple Close in aminoacid containing methionine, lysine, glycine, glutamic acid, proline and phenylalanine.
CN201610430323.3A 2016-06-17 2016-06-17 A kind of method utilizing Fructus Trichosanthis tuber quickly breeding high-quality seedling Pending CN106069021A (en)

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CN107079677A (en) * 2017-03-13 2017-08-22 蚌埠清菲农业科技有限公司 A kind of method of promotion Snakegourd Fruit root division
CN107211709A (en) * 2017-07-04 2017-09-29 安徽省久点农产品开发有限公司 A kind of breeding method of Snakegourd Fruit seedling
CN109757177A (en) * 2019-03-19 2019-05-17 亳州学院 A kind of method for culturing seedlings of Snakegourd Fruit
CN111533600A (en) * 2020-05-07 2020-08-14 淮北师范大学 Induction nutrient solution and induction culture method for rehmannia root tuber

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CN105191797A (en) * 2015-09-29 2015-12-30 潜山县传文瓜子有限公司 Snakegourd fruit tissues culture method
CN105191771A (en) * 2015-09-29 2015-12-30 潜山县传文瓜子有限公司 Method for soilless culture of fructus trichosanthis
CN105359776A (en) * 2015-10-20 2016-03-02 魏晓露 Method for mulching film seedling culture and planting of purple potatoes
CN105660239A (en) * 2016-03-03 2016-06-15 朱业斌 Purple yam seedling growing cultivation medium and seedling growing method thereof

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CN105052671A (en) * 2015-07-28 2015-11-18 江苏省铜山中等专业学校 Method for transplanting large cinnamomum camphora tree to land in north
CN105191797A (en) * 2015-09-29 2015-12-30 潜山县传文瓜子有限公司 Snakegourd fruit tissues culture method
CN105191771A (en) * 2015-09-29 2015-12-30 潜山县传文瓜子有限公司 Method for soilless culture of fructus trichosanthis
CN105359776A (en) * 2015-10-20 2016-03-02 魏晓露 Method for mulching film seedling culture and planting of purple potatoes
CN105660239A (en) * 2016-03-03 2016-06-15 朱业斌 Purple yam seedling growing cultivation medium and seedling growing method thereof

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107079677A (en) * 2017-03-13 2017-08-22 蚌埠清菲农业科技有限公司 A kind of method of promotion Snakegourd Fruit root division
CN107211709A (en) * 2017-07-04 2017-09-29 安徽省久点农产品开发有限公司 A kind of breeding method of Snakegourd Fruit seedling
CN109757177A (en) * 2019-03-19 2019-05-17 亳州学院 A kind of method for culturing seedlings of Snakegourd Fruit
CN111533600A (en) * 2020-05-07 2020-08-14 淮北师范大学 Induction nutrient solution and induction culture method for rehmannia root tuber

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Application publication date: 20161109