CN106068757B - A kind of method of mutagenesis of wingceltis coloured silk leaf kind - Google Patents
A kind of method of mutagenesis of wingceltis coloured silk leaf kind Download PDFInfo
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- CN106068757B CN106068757B CN201610412366.9A CN201610412366A CN106068757B CN 106068757 B CN106068757 B CN 106068757B CN 201610412366 A CN201610412366 A CN 201610412366A CN 106068757 B CN106068757 B CN 106068757B
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- wingceltis
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H3/00—Processes for modifying phenotypes, e.g. symbiosis with bacteria
- A01H3/04—Processes for modifying phenotypes, e.g. symbiosis with bacteria by treatment with chemicals
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
It is simple and easy to do the invention discloses a kind of method of mutagenesis of wingceltis coloured silk leaf kind, using mass concentration 1.2 1.5%（w/v）Ethylmethane sulfonate solution to wingceltis germination seed carry out immersion treatment, and carry out corresponding moisturizing and shading setting, the leaf variegation plant of formation shows as leaf color flavescence, or there is yellow patch in leaf color, plant is short and small, the strain rate that makes a variation is high, reaches 1.67 3.00%, lays a good foundation to obtain the color leaf kind of wingceltis tree.
Description
Technical field
The present invention relates to the breeding field of plant coloured silk leaf kind, specifically a kind of mutagenesis side of wingceltis coloured silk leaf kind
Method.
Background technology
Wingceltis（Pteroceltis tatarinowii Maxim.）For Ulmaceae（Ulmaceae）, wingceltis category deciduous tree,
For the distinctive autogenus of China, rare species have been included in《Chinese rare extinction plants register》Rare class three-level protective plant, is stone
The vanguard tree seed of limestone mountain area green barren hill can also grow in granite, sandstone region, be the distinctive fiber seeds of China and calcium
The important indicator plant of matter soil has not replaceable science effect in terms of studying Ulmaceae systematic growth.The seeds Chang Sheng
In the Limestone Mountain sparse woods of mountain valley small stream side, sprout tillers is strong, long lifespan, and root system often spirals between rock slot stretching, extension, has and surpasses
Strong drought-resistant barren ability, China do not carry out system research to wingceltis Tree Species Resources so far, and wingceltis breeding is still in primary
Stage, artificial induction's coloured silk leaf kind are to obtain one of the important channel of plant new type or new germ plasm.
Invention content
To solve above-mentioned technical problem, the present invention provides a kind of method of mutagenesis of wingceltis coloured silk leaf kind, operations
It is easy, the wingceltis coloured silk leaf kind strain rate that mutagenesis obtains is high, and Mutagenic Effect is good.
In order to achieve the above objectives, the technical solution adopted in the present invention is：
A kind of method of mutagenesis of wingceltis coloured silk leaf kind, is achieved by the steps of：
1）It to the wingceltis seed collected at the beginning of 11 months, will sprout by the end of October through sand storage；
2）When seed has 70-80% to show money or valuables one carries unintentionally, use quality concentration 1.2%-1.5%（w/v）Ethylmethane sulfonate solution into
Row immersion treatment, soaking time 40min-60min pay attention to sealing and shading in soaking process, cleaned with warm water after the completion of mutagenesis,
Liquid effect is released, sows in hole tray, is routinely managed after emergence；
3）For seedling after mutagenic treatment one month, wingceltis leaf color gradually appears variation, stablizes to the variation of September part, leaf color
Variation is apparent.
Method is simple for induction wingceltis coloured silk leaf kind provided by the invention, using mass concentration 1.2%-1.5%（w/v）
Ethylmethane sulfonate solution to wingceltis germination seed carry out immersion treatment, and carry out corresponding moisturizing and shading setting, formed
Leaf variegation plant show as leaf color turn yellow or leaf color on have yellow patch, plant is short and small, and variation strain rate is high, reaches
1.67-3.00% lays a good foundation to obtain the color leaf kind of wingceltis tree.
Specific implementation mode
With reference to specific embodiment, the present invention will be described in detail：
A kind of method of mutagenesis of wingceltis coloured silk leaf kind, is achieved by the steps of：
1）It to the full wingceltis seed collected at the beginning of 11 months, will sprout by the end of October through sand storage；
2）Seed have 75% or so show money or valuables one carries unintentionally when, using control and 0.6%-2.1% ethylmethane sulfonates solution carry out at immersion
It manages, soaking time 40-60min, sealing and shading is paid attention in soaking process, realize moisturizing and prevent ethylmethane sulfonate from volatilizing,
It is cleaned with warm water after the completion of mutagenesis, releases liquid effect, sow in hole tray, routinely managed after emergence；
3）After mutagenic treatment, growth is suppressed seedling, and leaf color makes a variation, and shows as leaf color flavescence or blade
On have yellow patch, plant short and small.Investigation statistics are carried out to the shape performance of variation plant to September part, to handle strain leaf leaf color
The abnormal plant of performance is that emphasis observes object.The plant that the ethylmethane sulfonate solution treatment of a concentration of 0.9%-1.8% is crossed, energy
Plant enough to generate variation, that the ethylmethane sulfonate solution treatment of aberration rate 0.33-3.00%, a concentration of 1.2-1.5% is crossed, generate
Variable effects are especially prominent, and variation strain rate is 1.67-3.00%.
As can be seen from the table, in terms of Mutagenic Effect, 0.9%, 1.2%, 1.3%, 1.5%, 1.8% ethylmethane sulfonate pair
The induction of wingceltis germination seed is effective.It is shown from the death rate, as ethylmethane sulfonate concentration increases, the plant death rate
It is in rising trend, ethylmethane sulfonate it is a concentration of 2.1% when, the young plant death rate highest of processing time 1h reaches 96.37%；Leaching
It is smaller to steep influence of the time to the death rate.It is shown from plant aberration rate, in 13 concentration processing combinations, especially with 1.3% methyl
Sulfonic acid concentration handles the variation strain rate highest of 60min, reaches 3.00%, is combined for optimization process；Followed by with 1.5% methyl
Sulfonic acid concentration handles 50min and 1.3% ethylmethane sulfonate handles 50min, and variation strain rate is 2.33%；It is 1.2% first again
Base sulfonic acid concentration handles 60min, handles 40min and 1.5% ethylmethane sulfonate concentration with 1.3% ethylmethane sulfonate concentration
60min is handled, variation strain rate is 2.00%；Again with 1.2% ethylmethane sulfonate concentration processing 40min or 50min, with 1.5% first
It is 1.67% that base sulfonic acid concentration, which handles 40min aberration rates,.
Certainly, above description is not limitation of the present invention, and the present invention is also not limited to the example above, this technology neck
The variations, modifications, additions or substitutions that the technical staff in domain is made in the essential scope of the present invention should also belong to the present invention's
Protection domain.
Claims (1)
1. a kind of method of mutagenesis of wingceltis coloured silk leaf kind, which is characterized in that be achieved by the steps of：
1）It to the wingceltis seed collected at the beginning of 11 months, will sprout by the end of October through sand storage；
2）When seed has 70-80% to show money or valuables one carries unintentionally, the ethylmethane sulfonate solution of 1 .2%-1 .5% of use quality concentration is impregnated
It handles, soaking time 40min-60min, sealing and shading is paid attention in soaking process, is cleaned with warm water after the completion of mutagenesis, medicine is released
Liquid acts on, and sows in hole tray, is routinely managed after emergence；
3）By step 2）Mutagenic treatment, for hole tray insemination and emergence after one month, wingceltis leaf color gradually appears variation, becomes to September part
Different stabilization, leaf variegation are apparent.
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| CN201610412366.9A CN106068757B (en) | 2016-06-14 | 2016-06-14 | A kind of method of mutagenesis of wingceltis coloured silk leaf kind |
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| CN106068757A CN106068757A (en) | 2016-11-09 |
| CN106068757B true CN106068757B (en) | 2018-10-09 |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109452168A (en) * | 2018-12-21 | 2019-03-12 | 安徽省农业科学院农业工程研究所 | A kind of red efficient mutagenic breeding method of beech leaf color mutant |
| CN113661921A (en) * | 2021-08-14 | 2021-11-19 | 泰安市泰山林业科学研究院 | Cultivation method of chionanthus roseus based on ethidium bromide and ultraviolet irradiation mutagenesis |
| CN114041376B (en) * | 2021-11-09 | 2023-01-10 | 安徽南北现代林业科技有限公司 | Seedling raising method for pteroceltis tatarinowii |
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| CN1439244A (en) * | 2003-03-20 | 2003-09-03 | 浙江大学 | Culture method for lawn grass with colour leaves |
| EA004572B1 (en) * | 1998-07-01 | 2004-06-24 | Джонс Хопкинс Скул Оф Медисин | Method of selecting crucifer plant with high level of anticancer activity, and progeny of said plant, and method for preparing sprouts of crucifer plant |
| CN102823403A (en) * | 2012-08-31 | 2012-12-19 | 泰安市泰山林业科学研究院 | Breeding method of pteroceltis tatarinowii polyploid |
| CN103340150A (en) * | 2013-07-19 | 2013-10-09 | 广东省农业科学院作物研究所 | Method for mutagenizing barbadosnut seeds by ethylmethane sulfonate |
| CN104542265A (en) * | 2013-10-25 | 2015-04-29 | 北京林业大学 | Mutation breeding method for cunninghamia lanceolata |
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- 2016-06-14 CN CN201610412366.9A patent/CN106068757B/en active Active
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| EA004572B1 (en) * | 1998-07-01 | 2004-06-24 | Джонс Хопкинс Скул Оф Медисин | Method of selecting crucifer plant with high level of anticancer activity, and progeny of said plant, and method for preparing sprouts of crucifer plant |
| CN1439244A (en) * | 2003-03-20 | 2003-09-03 | 浙江大学 | Culture method for lawn grass with colour leaves |
| CN102823403A (en) * | 2012-08-31 | 2012-12-19 | 泰安市泰山林业科学研究院 | Breeding method of pteroceltis tatarinowii polyploid |
| CN103340150A (en) * | 2013-07-19 | 2013-10-09 | 广东省农业科学院作物研究所 | Method for mutagenizing barbadosnut seeds by ethylmethane sulfonate |
| CN104542265A (en) * | 2013-10-25 | 2015-04-29 | 北京林业大学 | Mutation breeding method for cunninghamia lanceolata |
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| 甲基磺酸乙酯( EMS)处理对麻疯树(Jatropha curcas L .)萌发种子的生理效应;徐刚 等;《种子》;20101031;第29卷(第10期);49-53 * |
| 青檀多倍体诱导试验初报;张林 等;《中国农学通报》;20151231;第31卷(第13期);1-4 * |
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Inventor after: Zhang Lin Inventor after: Wei Zhonggang Inventor after: Zhu Cuicui Inventor after: Wang Changxian Inventor after: Wang Feng Inventor after: Sun Zhongkui Inventor after: Cheng Tiantian Inventor after: Zhang Anqi Inventor after: Li Chengxiu Inventor after: Wang Bo Inventor before: Zhang Lin Inventor before: Xie Xian Inventor before: Zhu Cuicui Inventor before: Wang Changxian Inventor before: Wang Feng Inventor before: Sun Zhongkui Inventor before: Cheng Tiantian Inventor before: Zhang Anqi Inventor before: Li Chengxiu Inventor before: Wang Bo |
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