CN106068757A - A kind of method of mutagenesis of wingceltis coloured silk leaf kind - Google Patents
A kind of method of mutagenesis of wingceltis coloured silk leaf kind Download PDFInfo
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- CN106068757A CN106068757A CN201610412366.9A CN201610412366A CN106068757A CN 106068757 A CN106068757 A CN 106068757A CN 201610412366 A CN201610412366 A CN 201610412366A CN 106068757 A CN106068757 A CN 106068757A
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- wingceltis
- leaf
- coloured silk
- mutagenesis
- plant
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H3/00—Processes for modifying phenotypes, e.g. symbiosis with bacteria
- A01H3/04—Processes for modifying phenotypes, e.g. symbiosis with bacteria by treatment with chemicals
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses the method for mutagenesis of a kind of wingceltis coloured silk leaf kind, simple and easy to do, use mass concentration 1.2 1.5%(w/v) ethylmethane sulfonate solution wingceltis germination seed is carried out immersion treatment, and carrying out corresponding moisturizing and shading setting, it is yellow that the leaf variegation plant of formation shows as leaf complexion changed, or has yellow speckle on leaf color, plant is short and small, variant rate is high, reaches 1.67 3.00%, lays a good foundation for obtaining the color leaf kind of wingceltis tree.
Description
Technical field
The present invention relates to the breeding field of plant coloured silk leaf kind, the mutation side of a kind of wingceltis coloured silk leaf kind
Method.
Background technology
Wingceltis (Pteroceltis tatarinowii Maxim.) is Ulmaceae (Ulmaceae), and wingceltis belongs to deciduous tree,
For Chinese distinctive autogenus, rare species, list " China's rare extinction plants register " rare class three-level protective plant in, be stone
The vanguard tree seed of limestone mountain area green barren hill, can be also Chinese distinctive fiber seeds and calcium in granite, sandstone region growth
The important indicator plant of matter soil, has the most replaceable science effect in terms of research Ulmaceae phylogeny.This seeds Chang Sheng
In the Limestone Mountain sparse woods of small stream limit, mountain valley, sprout tillers is strong, the life-span is long, and root system often spirals stretching, extension between rock slot, has super
Strong drought-resistant barren ability, wingceltis Tree Species Resources was not the most carried out systematic study by China, and wingceltis breeding is still in primary
In the stage, artificial induction's coloured silk leaf kind is one of important channel obtaining the new type of plant or new germ plasm.
Summary of the invention
For solving the technical problem of above-mentioned existence, the invention provides the method for mutagenesis of a kind of wingceltis coloured silk leaf kind, operation
Easily, the wingceltis coloured silk leaf kind strain rate that mutation obtains is high, and Mutagenic Effect is good.
For reaching above-mentioned purpose, the technical solution adopted in the present invention is:
The method of mutagenesis of a kind of wingceltis coloured silk leaf kind, is achieved by the steps of:
1) by by the end of October to the wingceltis seed collected at the beginning of 11 months, hide through sand and sprout；
2) when seed has 70-80% to show money or valuables one carries unintentionally, use mass concentration 1.2%-1.5%(w/v) ethylmethane sulfonate solution soak
Bubble processes, soak time 40min-60min, notes sealing and shading in immersion process, and mutation is cleaned with warm water after completing, and releases
Medicinal liquid effect, sowing, in hole tray, manages after emerging routinely；
3) seedling is after mutagenic treatment one month, and wingceltis leaf color engenders variation, stable to JIUYUE variation, leaf variegation
Substantially.
Method is simple for the induction wingceltis coloured silk leaf kind that the present invention provides, and uses mass concentration 1.2%-1.5%(w/v)
Ethylmethane sulfonate solution wingceltis germination seed is carried out immersion treatment, and carry out corresponding moisturizing and shading is arranged, formed
Leaf variegation plant to show as leaf complexion changed yellow, or on leaf color, have yellow speckle, plant is short and small, and variant rate is high, reaches
1.67-3.00%, lays a good foundation for obtaining the color leaf kind of wingceltis tree.
Detailed description of the invention
Describe the present invention below in conjunction with specific embodiment:
The method of mutagenesis of a kind of wingceltis coloured silk leaf kind, is achieved by the steps of:
1) by by the end of October to the full wingceltis seed collected at the beginning of 11 months, hide through sand and sprout；
2) when seed has about 75% to show money or valuables one carries unintentionally, comparison and 0.6%-2.1% ethylmethane sulfonate solution is used to carry out immersion treatment,
Soak time 40-60min, notes in immersion process sealing and shading, it is achieved moisturizing and prevent ethylmethane sulfonate from volatilizing, mutation
Cleaning with warm water after completing, release medicinal liquid effect, sowing, in hole tray, manages after emerging routinely；
3) seedling is after mutagenic treatment, and growth is suppressed, and variation occurs in leaf color, shows as leaf complexion changed yellow, or has on blade
Yellow speckle, plant is short and small.To JIUYUE, the shape performance of variation plant is carried out investigation statistics, existing to process strain leaf leaf color table
Abnormal plant is attached most importance to the object of observation.Concentration is the plant that the ethylmethane sulfonate solution of 0.9%-1.8% processed, it is possible to produce
Changing different, aberration rate 0.33-3.00%, concentration is the plant that the ethylmethane sulfonate solution of 1.2-1.5% processed, and produces variation
Effect is especially prominent, and variant rate is 1.67-3.00%.
As can be seen from the table, in terms of Mutagenic Effect, the ethylmethane sulfonate pair of 0.9%, 1.2%, 1.3%, 1.5%, 1.8%
The induction of wingceltis germination seed is the most effective.Show from mortality rate, along with ethylmethane sulfonate concentration raises, plant mortality rate
In rising trend, when ethylmethane sulfonate concentration is 2.1%, the young plant mortality rate processing time 1h is the highest, reaches 96.37%；Leaching
The bubble time is less on the impact of mortality rate.Show from plant aberration rate, in 13 concentration process combination, especially with 1.3% methyl
The variant rate that sulfonic acid concentration processes 60min is the highest, reaches 3.00%, combines for optimization process；Next to that with 1.5% methyl
Sulfonic acid concentration processes 50min and 1.3% ethylmethane sulfonate processes 50min, and variant rate is 2.33%；It is 1.2% first again
Base sulfonic acid concentration processes 60min, processes 40min and 1.5% ethylmethane sulfonate concentration with 1.3% ethylmethane sulfonate concentration
Processing 60min, variant rate is 2.00%；Again process 40min or 50min with 1.2% ethylmethane sulfonate concentration, with 1.5% first
It is 1.67% that base sulfonic acid concentration processes 40min aberration rate.
Certainly, described above is not limitation of the present invention, and the present invention is also not limited to the example above, and this technology is led
Change that the technical staff in territory is made in the essential scope of the present invention, retrofit, add or replace, also should belong to the present invention's
Protection domain.
Claims (1)
1. the method for mutagenesis of a wingceltis coloured silk leaf kind, it is characterised in that be achieved by the steps of:
1) by by the end of October to the wingceltis seed collected at the beginning of 11 months, hide through sand and sprout；
2) when seed has 70-80% to show money or valuables one carries unintentionally, the ethylmethane sulfonate solution of mass concentration 1.2%-1.5% is used to carry out at immersion
Reason, soak time 40min-60min, immersion process notes seal and shading, mutation is cleaned with warm water after completing, and releases medicinal liquid
Effect, sowing, in hole tray, manages after emerging routinely；
3) seedling is after mutagenic treatment one month, and wingceltis leaf color engenders variation, stable to JIUYUE variation, leaf variegation
Substantially.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610412366.9A CN106068757B (en) | 2016-06-14 | 2016-06-14 | A kind of method of mutagenesis of wingceltis coloured silk leaf kind |
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| CN201610412366.9A CN106068757B (en) | 2016-06-14 | 2016-06-14 | A kind of method of mutagenesis of wingceltis coloured silk leaf kind |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106068757A true CN106068757A (en) | 2016-11-09 |
| CN106068757B CN106068757B (en) | 2018-10-09 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109452168A (en) * | 2018-12-21 | 2019-03-12 | 安徽省农业科学院农业工程研究所 | A kind of red efficient mutagenic breeding method of beech leaf color mutant |
| CN113661921A (en) * | 2021-08-14 | 2021-11-19 | 泰安市泰山林业科学研究院 | Cultivation method of chionanthus roseus based on ethidium bromide and ultraviolet irradiation mutagenesis |
| CN114041376A (en) * | 2021-11-09 | 2022-02-15 | 安徽南北现代林业科技有限公司 | Seedling raising method for pteroceltis tatarinowii |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109452168A (en) * | 2018-12-21 | 2019-03-12 | 安徽省农业科学院农业工程研究所 | A kind of red efficient mutagenic breeding method of beech leaf color mutant |
| CN113661921A (en) * | 2021-08-14 | 2021-11-19 | 泰安市泰山林业科学研究院 | Cultivation method of chionanthus roseus based on ethidium bromide and ultraviolet irradiation mutagenesis |
| CN114041376A (en) * | 2021-11-09 | 2022-02-15 | 安徽南北现代林业科技有限公司 | Seedling raising method for pteroceltis tatarinowii |
| CN114041376B (en) * | 2021-11-09 | 2023-01-10 | 安徽南北现代林业科技有限公司 | Seedling raising method for pteroceltis tatarinowii |
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| CN106068757B (en) | 2018-10-09 |
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Inventor after: Zhang Lin Inventor after: Wei Zhonggang Inventor after: Zhu Cuicui Inventor after: Wang Changxian Inventor after: Wang Feng Inventor after: Sun Zhongkui Inventor after: Cheng Tiantian Inventor after: Zhang Anqi Inventor after: Li Chengxiu Inventor after: Wang Bo Inventor before: Zhang Lin Inventor before: Xie Xian Inventor before: Zhu Cuicui Inventor before: Wang Changxian Inventor before: Wang Feng Inventor before: Sun Zhongkui Inventor before: Cheng Tiantian Inventor before: Zhang Anqi Inventor before: Li Chengxiu Inventor before: Wang Bo |
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