CN104255482A - Method for quickly reproducing Koelreuteria paniculata by tissue culture - Google Patents
Method for quickly reproducing Koelreuteria paniculata by tissue culture Download PDFInfo
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- CN104255482A CN104255482A CN201410463255.1A CN201410463255A CN104255482A CN 104255482 A CN104255482 A CN 104255482A CN 201410463255 A CN201410463255 A CN 201410463255A CN 104255482 A CN104255482 A CN 104255482A
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Abstract
The invention provides a method for quickly reproducing Koelreuteria paniculata by tissue culture. The method comprises the following steps: preparing seedlings of Koelreuteria paniculata, multiplying multiple shoots, rooting, acclimatizing and transplanting and the like. The Koelreuteria paniculata prepared by the method is simple to operate and high in value-added ratio and the method can be used for preparing a large quantity of Koelreuteria paniculata seedlings in a short period.
Description
Technical field
The present invention relates to the quick-breeding method of Luan Shu tissue cultures, belong to plant technology field.
Background technology
Luan Shu, another name: wooden goldenrain tree, Luan Hua, five Wu Laye, Wu La, crow draw glue, black Color-leaf tree, Shi Luanshu, black Ye Shu, wooden hurdle tooth, Latin literary fame:
koelreuteria paniculata, Sapindaceae, is grown in the calcium base soil of lime stone weathering generation, and it is the plant of a kind of happiness light, slightly resistance to half shade; Cold-resistant; But not resistance to water logging, attention soil of planting, drought-resistant and barren.Luan Shu has deep-rootedness, and sprout tiller strength is strong, and growth rate is medium, and sapling up-growth is comparatively slow, later gradually fast, has stronger anti-flue dust ability.Based on seed propagation, tiller or root insert also can.Because seed coat is hard not easily permeable, if not treated, Second Year is sowed in spring and is not often germinateed, therefore sowing of shelling autumn, sow in spring after available wet husky Stratificated treatment.General employing bed planting, ridge is apart from 60-70cm, and because seeding ratio is low, therefore sowing quantity is large, seeding quantity 30-40kg/ mu.Luan Shu originates in NORTH CHINA and middle part, and also there are distribution in Japan, Korea.There is distribution in the whole nation more, and comparatively common with Central China, East China, is distributed in low mountain and the Plain of height above sea level less than 1500 meters more.Main breeding base has Jiangsu, Zhejiang, Jiangxi, Anhui, and Henan is also one of Luan Shu production base.Normal cultivation views and admires tree as flower garden.Timber yellow-white, easily processes, can furniture processed; Leaf can make blue dyes, flower hyoscine.Micro-hardship, pungent.Function cures mainly: wind and heat dispersing, cough-relieving, desinsection, and the main routine that adopts is planted at present, and tissue culture method can obtain a large amount of Luan Shu seedling fast, keeps the stability of genetic character, overcomes the limitation of region.
Summary of the invention
Technical problem to be solved by this invention is to provide the method for quickly breeding of a kind of Luan Shu, Luan Shu prepared by the method, and simple to operate, appreciation rate is high, can obtain a large amount of Luan Shu seedling in a short time.
Technical problem to be solved by this invention is realized by following scheme:
The alcohol of 70% filters out full seed, then add in running water with the concentrated sulfuric acid that concentration is 0.8mol/L, soak Luan Shu seed 30min, clear water rinses 4-5 time, seed sprouting after one week, cultivation a period of time cuts goldenrain tree usage tree root and unnecessary blade, disinfection, access culture medium prescription is the propagation of carrying out Multiple Buds in the medium of WPM+0.2mg/L6-BA+0.1mg/LZT, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 2000lx, temperature 25 DEG C, the Multiple Buds cultivated out is put into medium 1/3WPM+NAA0.1mg/L+IAA0.1mg/L and is carried out root induction, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 8000lx, temperature 27 DEG C, treat that test-tube plantlet is about 5cm, root is about 2cm, wash away root medium, 2min is soaked in 10% antiformin solution, running water is clean, to plant the pine forest humus soil crossed in high-temperature sterilization: in the matrix of bark powder=3:1, regular water spray, spray the potassium dihydrogen phosphate growth promoting effects of a 1:250 week about, temperature 25 DEG C, black shading screen hides, humidity 80%.
The Luan Shu survival rate adopting the present invention to prepare is 90%, and the cycle is short, and output is large, pollutes little, is beneficial to implant mass.
Below in conjunction with embodiment, the present invention is further elaborated, but the scope of protection of present invention is not limited to following embodiments.
Embodiment
Embodiment 1
The alcohol of 70% filters out full seed, then add in running water with the concentrated sulfuric acid that concentration is 0.8mol/L, soak Luan Shu seed 30min, clear water rinses 4-5 time, seed sprouting after one week, cultivation a period of time cuts goldenrain tree usage tree root and unnecessary blade, disinfection, access culture medium prescription is the propagation of carrying out Multiple Buds in the medium of WPM+0.2mg/L6-BA+0.1mg/LZT, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 2000lx, temperature 25 DEG C, the Multiple Buds cultivated out is put into medium 1/3WPM+NAA0.05mg/L+IAA0.05mg/L and is carried out root induction, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 8000lx, temperature 27 DEG C, treat that test-tube plantlet is about 5cm, root is about 2cm, wash away root medium, 2min is soaked in 10% antiformin solution, running water is clean, to plant the pine forest humus soil crossed in high-temperature sterilization: in the matrix of bark powder=3:1, regular water spray, spray the potassium dihydrogen phosphate growth promoting effects of a 1:250 week about, temperature 25 DEG C, black shading screen hides, humidity 80%, survival rate is 87%.
Embodiment 2
The alcohol of 70% filters out full seed, then add in running water with the concentrated sulfuric acid that concentration is 0.8mol/L, soak Luan Shu seed 30min, clear water rinses 4-5 time, seed sprouting after one week, cultivation a period of time cuts goldenrain tree usage tree root and unnecessary blade, disinfection, access culture medium prescription is the propagation of carrying out Multiple Buds in the medium of WPM+0.25mg/L6-BA+0.1mg/LZT, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 2000lx, temperature 25 DEG C, the Multiple Buds cultivated out is put into medium 1/3WPM+NAA0.1mg/L+IAA0.1mg/L and is carried out root induction, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 8000lx, temperature 27 DEG C, treat that test-tube plantlet is about 5cm, root is about 2cm, wash away root medium, 2min is soaked in 10% antiformin solution, running water is clean, to plant the pine forest humus soil crossed in high-temperature sterilization: in the matrix of bark powder=3:1, regular water spray, spray the potassium dihydrogen phosphate growth promoting effects of a 1:250 week about, temperature 25 DEG C, black shading screen hides, humidity 80%, survival rate is 89%.
Embodiment 3
The alcohol of 70% filters out full seed, then add in running water with the concentrated sulfuric acid that concentration is 0.8mol/L, soak Luan Shu seed 30min, clear water rinses 4-5 time, seed sprouting after one week, cultivation a period of time cuts goldenrain tree usage tree root and unnecessary blade, disinfection, access culture medium prescription is the propagation of carrying out Multiple Buds in the medium of WPM+0.2mg/L6-BA+0.1mg/LZT, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 2000lx, temperature 25 DEG C, the Multiple Buds cultivated out is put into medium 1/3WPM+NAA0.1mg/L+IAA0.05mg/L and is carried out root induction, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 8000lx, temperature 27 DEG C, treat that test-tube plantlet is about 5cm, root is about 2cm, wash away root medium, 2min is soaked in 10% antiformin solution, running water is clean, to plant the pine forest humus soil crossed in high-temperature sterilization: in the matrix of bark powder=3:1, regular water spray, spray the potassium dihydrogen phosphate growth promoting effects of a 1:250 week about, temperature 25 DEG C, black shading screen hides, humidity 80%, survival rate is 88%.
Claims (4)
1. a method for quickly breeding for Luan Shu tissue cultures, comprise the acquisition of Luan Shu seedling, the propagation of Multiple Buds, culture of rootage, acclimatization and transplants, its key step is as follows:
(1) get the seed of Luan Shu, 70% alcohol sieve, uses sulfuric acid to soften seed coat, obtains the seedling of Luan Shu;
(2) get step (1) cultivate a period of time cut goldenrain tree usage tree root and unnecessary blade, disinfection, access culture medium prescription is the propagation of carrying out Multiple Buds in the medium of WPM+0.2-0.25mg/L6-BA+0.1mg/LZT, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 2000lx, temperature 25 DEG C;
(3) get step (2) to cultivate Multiple Buds out and put into medium 1/3WPM+NAA0.05-0.1mg/L+IAA0.05-0.1mg/L and carry out root induction, additional saccharose 30g/L, agar 6.5g/L, pH5.8, illumination 8000lx, temperature 27 DEG C;
(4) get step (3) take root after test-tube plantlet carry out acclimatization and transplants.
2. according to the method for quickly breeding of a kind of Luan Shu tissue cultures according to claim 1, it is characterized in that: the acquisition of Luan Shu seedling described in step (1) is, alcohol with 70% filters out full seed, then add in running water with the concentrated sulfuric acid that concentration is 0.8mol/L, soak Luan Shu seed 30min, clear water rinses 4-5 time, observes germination after one week.
3. according to the method for quickly breeding of a kind of Luan Shu tissue cultures according to claim 1, it is characterized in that: in step (2), the sterilization method of Luan Shu seedling stem section is, running water 10min, 0.1% mercuric chloride process 3min, running water 5 times.
4. according to the method for quickly breeding of a kind of Luan Shu tissue cultures according to claim 1, it is characterized in that: in step (4), the acclimatization and transplants method of Luan Shu is about 5cm for getting seedling, root is about the test-tube plantlet of 2cm, wash away root medium, 2min is soaked in 10% antiformin solution, running water is clean, to plant the pine forest humus soil crossed in high-temperature sterilization: in the matrix of bark powder=3:1, regular water spray, spray the potassium dihydrogen phosphate growth promoting effects of a 1:250 week about, temperature 25 DEG C, black shading screen hides, humidity 80%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201410463255.1A CN104255482A (en) | 2014-09-12 | 2014-09-12 | Method for quickly reproducing Koelreuteria paniculata by tissue culture |
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| CN201410463255.1A CN104255482A (en) | 2014-09-12 | 2014-09-12 | Method for quickly reproducing Koelreuteria paniculata by tissue culture |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104620934A (en) * | 2015-03-04 | 2015-05-20 | 南京金埔园林股份有限公司 | Big seedling transplantation method for goldenrain trees |
| CN105724256A (en) * | 2015-05-07 | 2016-07-06 | 江苏省林业科学研究院 | Mini-type grafting method of Koelreuteria bipinnata var. integrifoliola 'Jinyan' |
| CN106376458A (en) * | 2016-08-19 | 2017-02-08 | 江苏艺轩园林景观工程有限公司 | Koelreuteria paniculata breeding method |
-
2014
- 2014-09-12 CN CN201410463255.1A patent/CN104255482A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104620934A (en) * | 2015-03-04 | 2015-05-20 | 南京金埔园林股份有限公司 | Big seedling transplantation method for goldenrain trees |
| CN104620934B (en) * | 2015-03-04 | 2017-04-05 | 金埔园林股份有限公司 | A kind of tree transplanted method of Luan Shu |
| CN105724256A (en) * | 2015-05-07 | 2016-07-06 | 江苏省林业科学研究院 | Mini-type grafting method of Koelreuteria bipinnata var. integrifoliola 'Jinyan' |
| CN106376458A (en) * | 2016-08-19 | 2017-02-08 | 江苏艺轩园林景观工程有限公司 | Koelreuteria paniculata breeding method |
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Application publication date: 20150107 |
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