CN105878188A - Fermented cordyceps sinensis extracellular polysaccharide granules and preparation method thereof - Google Patents
Fermented cordyceps sinensis extracellular polysaccharide granules and preparation method thereof Download PDFInfo
- Publication number
- CN105878188A CN105878188A CN201410618258.8A CN201410618258A CN105878188A CN 105878188 A CN105878188 A CN 105878188A CN 201410618258 A CN201410618258 A CN 201410618258A CN 105878188 A CN105878188 A CN 105878188A
- Authority
- CN
- China
- Prior art keywords
- fermentation
- extracellular polysaccharide
- cordyceps
- granule
- cordyceps sinensis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to fermented cordyceps sinensis extracellular polysaccharide granules and a preparation method thereof, aims to solve the problems that a fermentation liquid obtained after cordyceps sinensis mycelium production is discarded, resources, energy sources, manpower, material resources and financial resources are wasted and pollution to surrounding water bodies and soil exists, and meets the requirements of markets on products rich in a cordyceps sinensis extracellular polysaccharide component. The preparation method comprises the steps: carrying out strain acquisition, strain cultivation, asepsis separation, low-temperature cultivation, purification, strain identification, original strain preservation, fermentation performance testing, liquid fermentation, bacterial liquid separation, fermentation liquid concentration, and extraction and refining of cordyceps sinensis extracellular crude polysaccharide; taking fermented cordyceps sinensis extracellular polysaccharide related auxiliary materials, evenly mixing and then sieving; and preparing a soft material, granulating, drying, carrying out size stabilization, and thus obtaining the fermented cordyceps sinensis extracellular polysaccharide granules. The product has the functions of resisting tumor, resisting oxidation, enhancing the phagocytic ability of mononuclear macrophage, increasing the content of serum IgG, promoting the transformation of in-vitro lymphocytes, resisting radiation and the like.
Description
Technical field
The present invention relates to a kind of fermentation Cordyceps extracellular polysaccharide granule and preparation method thereof, specifically,
Refer to fermentation Cordyceps extracellular polysaccharide is carried out granule and the preparation side thereof that isolated and purified interpolation adjuvant prepares
Method.
Background technology
Fermentation Cordyceps fungus powder be through strain collection, spawn culture, aseptic separation, Low-temperature culture, purification,
Strain identification, original strain preservation, fermenting property mensuration, liquid fermentation, bacterium solution separation, mycelium are dried
Prepare etc. technique.And fermentation liquid is often processed as waste liquid, owing to fermentation liquid containing a large amount of microorganisms
With other functional components, surrounding water and soil can be polluted, need through High Temperature High Pressure by direct discharge
Sterilization treatment just can be discharged, and not only wastes ample resources, the energy, human and material resources and financial resources, and
Be there is potential safety hazard in surrounding.
Test shows, fermentation Cordyceps extracellular polysaccharide have antitumor, antioxidation, enhancing monokaryon huge bite thin
In the phagocytic activity of born of the same parents, raising serum, the content of IgG, promotion lymphocytes in vitro conversion and radioprotective etc. are made
With.And Cordyceps fermented liquid also contains several functions effective ingredient, as having inhibitory action many pathogenic bacteria
Sugar, Cordycepin, many peptides, mannitol, monosaccharide, organic acid, polyamines, phosphatidylcholine, trace unit
The compositions such as element and VB12.Their physiologically active is the most rich and varied, can antibacterial, anticancer, antiplatelet coagulate
Knot, radioprotective, improve memory, regulation body immunity, calcium ion antagonism, to brain and the heart
Dirty protected effect, calmness and analgesia etc. under normobaric hypoxia.
Research and production currently for fermentation Cordyceps extracellular polysaccharide belong to blank.
On this basis, the enforcement of the present invention, provide not only and there is antitumor, enhancing mononuclear phagocyte
Phagocytic activity, improve the content of IgG in mice serum, lymphocytes in vitro is converted have facilitation and
The extracellular polysaccharide granule product of the effect such as radioprotective, make use of Cordyceps fermented liquid to greatest extent, becomes
Waste be changed into values, has also reached to economize on resources, the energy, human and material resources and financial resources, reduces the purpose of environmental pollution.
Summary of the invention
For solving the fermentation liquid discard processing after artificial fermentation Cordyceps mode produces Cordyceps mycelium,
Waste resource, the energy, human and material resources and financial resources, and exist surrounding water and the problem of soil pollution,
And for meeting market to the demand rich in extracellular polysaccharide component product, it is an object of the invention to provide a kind of profit
Produce the fermentation liquid after Cordyceps mycelium by artificial fermentation Cordyceps mode and produce outside Cordyceps born of the same parents many
Sugar granule and preparation method thereof.The method produce Cordyceps extracellular polysaccharide granule have antitumor,
Antioxidation, strengthen the phagocytic activity of mononuclear phagocyte, improve the content of IgG in serum, promote external pouring
Bar cell transformation and radioprotective etc. act on.
For reaching above-mentioned purpose, the present invention provides one to utilize artificial fermentation Cordyceps mode to produce the worm summer in winter
Grass mycelium after fermentation liquid produce Cordyceps extracellular polysaccharide granule, include: content between 20% to
Fermentation Cordyceps extracellular polysaccharide, the content of 55% percentage by weight are between 20% to 60% percentage by weight
Filler, content between the slow-release material of 5% to 20% percentage by weight, content between 1% to 5% weight hundred
The binding agent of proportion by subtraction, content between the disintegrating agent of 0.5% to 1% percentage by weight and content between 0.1%
Correctives to 0.5% percentage by weight.
Described filler is selected from the one in sucrose or soluble starch or lactose or dextrin or microcrystalline Cellulose
Or multiple mixture；Described slow-release material is selected from hydroxypropyl methylcellulose or ethyl cellulose or acetate fiber
One or more mixture in element or Aquacoat；Described binding agent is selected from dextrin or poly-
Tie up one or more mixture in ketone or ethyl cellulose or hydroxypropyl cellulose；Described disintegrating agent is selected from
In carboxymethyl starch sodium or microcrystalline Cellulose or cross-linking sodium carboxymethyl cellulose or low-substituted hydroxypropyl cellulose
One or more mixture；One or both mixing in stevioside or aspartame of described correctives
Thing；Described wetting agent one or both mixture in distilled water or edible ethanol.
Preferably, the Cordyceps extracellular polysaccharide granule that is used for fermenting includes:
Content is the fermentation Cordyceps extracellular polysaccharide of 44% percentage by weight, the sieve powder of 80 to 100；
Content is the filler of 40% percentage by weight, and described filler is between 20% to 60% weight by content
The lactose of amount percentage ratio adds the content soluble starch mix homogeneously between 40% to 80% percentage by weight,
Pulverize and sieve, the sieve powder of 80 to 100；
Content is the slow-release material of 12% percentage by weight, described slow-release material be by content between 10% to
The hydroxypropyl methyl cellulose of 30% percentage by weight, content are between the ethyl of 20% to 60% percentage by weight
Cellulose, content, between the cellulose acetate mix homogeneously of 10% to 70% percentage by weight, pulverize and sieve,
The sieve powder of 80 to 100；
Content is the binding agent of 3% percentage by weight, and described binding agent is to be pulverized and sieved by polyvidone, 80 to
The sieve powder of 100；
Content is the disintegrating agent of 0.7% percentage by weight, and described disintegrating agent is to pulverize microcrystalline Cellulose to sieve,
The sieve powder of 80 to 100；And
Content is the correctives of 0.3% percentage by weight, and described correctives is to be pulverized and sieved by stevioside, 80 to
The sieve powder of 100.
The present invention further relates in the extracting method of fermentation Cordyceps extracellular polysaccharide.
The extracting method of the fermentation Cordyceps extracellular polysaccharide of the present invention, includes: utilize Cordyceps strain,
Liquid fermentation produces Cordyceps mycelium；After fermentation ends, extract mycelium and collect fermentation liquid；Fermentation
Liquid external heating type Three-effect concentration device concentrates, each effect vacuum and evaporating temperature: an effect-0.02～-0.04MPa,
70～90 DEG C；Two effects-0.04～-0.06MPa, 60～80 DEG C；Triple effect-0.06～-0.08MPa, 50～70 DEG C, fermentation
Liquid is concentrated into relative density 1.20～1.35；The pH of the fermentation liquid after concentrating is adjusted to 6.5～8.5, and room temperature stands
1～4h, 3000r/min～10000r/min be centrifuged 5～20min removal precipitation；By the edible ethanol of 75%～95%
Paste in the concentrated broth that chamber wall adds 40r/min～500r/min with the flow velocity of 1L/min～10L/min, send out
When in ferment liquid, concentration of alcohol reaches 60%～75%, stopping adds, and stops stirring, same after 0～20 DEG C of standing 8～12h
Receiving supernatant and collect precipitation, precipitation is Cordyceps crude extracellular polysaccharide；The crude polysaccharides of gained is carried
Pure, dry, pulverize and sieve, must ferment Cordyceps extracellular polysaccharide.
Preferably, the method being used for preparing fermentation Cordyceps extracellular polysaccharide includes:
Utilizing Cordyceps strain, liquid fermentation produces Cordyceps mycelium；
After fermentation ends, extract mycelium and collect fermentation liquid；Fermentation liquid external heating type Three-effect concentration device is dense
Contracting, each effect vacuum and evaporating temperature: an effect-0.025MPa, 80 DEG C；Two effect-0.05MPa, 70 DEG C；Three
Effect-0.07MPa, 60 DEG C, fermentation liquid is concentrated into relative density 1.28；
The pH of the fermentation liquid after concentrating is adjusted to 7.0, and room temperature stands 1.5h, 5000r/min and is centrifuged 10min and goes
Except precipitation；
After edible ethanol by 95% pastes, with the flow velocity of 6L/min, the removal precipitation that chamber wall adds 100r/min
Concentrated broth in, in fermentation liquid, concentration of alcohol stops adding when reaching 60%, stops stirring, 4 DEG C of standings
Reclaiming supernatant after 8h and collect precipitation, precipitation is Cordyceps crude extracellular polysaccharide；
Purifying the crude polysaccharides of gained, dry, pulverize and sieve, must ferment Cordyceps extracellular polysaccharide.
The present invention is also on the preparation method of fermentation Cordyceps extracellular polysaccharide granule.
The preparation method of the fermentation Cordyceps extracellular polysaccharide granule of the present invention, includes: take fermentation winter worm
Summer grass extracellular polysaccharide, filler, slow-release material, disintegrating agent, correctives, sieves after mix homogeneously, takes 80～100
Mesh sieve powder；Binding agent is added content be said components gross weight 5～25% wetting agent in, described wetting agent
It is with the edible ethanol that distilled water diluting to concentration is 60%～80%；Wetting agent mixes soft material processed with sieve powder, system
Grain, 30～80 DEG C are dried 2～12 hours, sieve, take 20～40 mesh sieve powder, must ferment outside Cordyceps born of the same parents
Polyoses grain agent.
Preferably, the method being used for preparing fermentation Cordyceps extracellular polysaccharide granule includes:
Taking fermentation Cordyceps extracellular polysaccharide, filler, slow-release material, disintegrating agent, correctives, mixing is all
Sieve after even, take 100 mesh sieve powder；
Binding agent is added content fermentation Cordyceps extracellular polysaccharide, filler, slow-release material, disintegrating agent,
In the wetting agent of correctives and binding agent gross weight 20% mass percent, described wetting agent is dilute with distilled water
Release to concentration be the edible ethanol of 60%；
Wetting agent mixes soft material processed with sieve powder, pelletizes；
60 DEG C are dried 8 hours, sieve, take 30 mesh sieve powder, and must ferment Cordyceps extracellular polysaccharide granule.
The fermentation Cordyceps extracellular polysaccharide granule being suitable for commercially producing can be utilized by the present invention, and then
There is provided fermentation Cordyceps deep processed product to meet the market demand, reach the purpose of the present invention.
Detailed description of the invention
Following example are used for illustrating the present invention, but are not limited to the scope of the present invention.
(1) Cordyceps extracellular polysaccharide granule
Embodiment 1
10.00kg fermentation Cordyceps extracellular polysaccharide granule includes:
5.00kg ferments Cordyceps extracellular polysaccharide, the sieve powder of 100；
The filler of 2.60kg, described filler is plus 2.08kg by the lactose of 0.52kg percentage by weight
Soluble starch mix homogeneously, pulverizes and sieves, the sieve powder of 100；
The slow-release material of 2.00kg, described slow-release material is by the hydroxypropyl methyl cellulose of 0.20kg, 0.40kg
Ethyl cellulose, the cellulose acetate mix homogeneously of 1.40kg, pulverize and sieve, the sieve powder of 100；
The binding agent of 0.30kg, described binding agent is to be pulverized and sieved by the polyvidone of 0.30kg, the sieve powder of 100:
The disintegrating agent of 0.075kg, described disintegrating agent is to pulverize the microcrystalline Cellulose of 0.075kg to sieve, 100
Sieve powder；And
The correctives of 0.025kg, described correctives is to be pulverized and sieved by the stevioside of 0.025kg, the sieve of 100
Powder.
Described Cordyceps extracellular polysaccharide granule has the activity of the superoxide dismutase of 21.58IU/g.
Embodiment 2
10.00kg fermentation Cordyceps extracellular polysaccharide granule includes:
2.00kg ferments Cordyceps extracellular polysaccharide, the sieve powder of 100；
The filler of 6.00kg, described filler is plus 4.80kg by the lactose of 1.20kg percentage by weight
Soluble starch mix homogeneously, pulverizes and sieves, the sieve powder of 100；
The slow-release material of 1.34kg, described slow-release material be by the hydroxypropyl methyl cellulose of 0.134kg,
The ethyl cellulose of 0.268kg, the cellulose acetate mix homogeneously of 0.938kg, pulverize and sieve, the sieve powder of 100；
The binding agent of 0.10kg, described binding agent is to be pulverized and sieved by the polyvidone of 0.30kg, the sieve powder of 100；
The disintegrating agent of 0.05kg, described disintegrating agent is to pulverize the microcrystalline Cellulose of 0.05kg to sieve, 100
Sieve powder；And
The correctives of 0.01kg, described correctives is to be pulverized and sieved by the stevioside of 0.01kg, the sieve powder of 100.
Described Cordyceps extracellular polysaccharide granule has the activity of the superoxide dismutase of 5.62IU/g.
(2) for the method preparing fermentation Cordyceps extracellular polysaccharide
Embodiment 3
Utilizing Cordyceps strain, liquid fermentation produces Cordyceps mycelium；
After fermentation ends, extract mycelium and collect fermentation liquid；Fermentation liquid external heating type Three-effect concentration device is dense
Contracting, each effect vacuum and evaporating temperature: an effect-0.04MPa, 70 DEG C；Two effect-0.06MPa, 60 DEG C: three
Effect-0.08MPa, 50 DEG C, fermentation liquid is concentrated into relative density 1.22；
The pH of the fermentation liquid after being concentrated by 50L is adjusted to 6.5, and room temperature stands 1.5h, 3000r/min and is centrifuged 20min
Remove precipitation；
After edible ethanol by 75% pastes, with the flow velocity of 1L/min, the removal precipitation that chamber wall adds 40 r/min
In concentrated broth, in fermentation liquid, concentration of alcohol reaches to stop adding when 60%, stops stirring, and 0 DEG C stands 8h
Rear recovery supernatant also collects precipitation, and precipitation is Cordyceps crude extracellular polysaccharide, obtains crude polysaccharides 3.62kg；
Purifying the crude polysaccharides of gained, dry, pulverize and sieve, must ferment Cordyceps extracellular polysaccharide.
Embodiment 4
Utilizing Cordyceps strain, liquid fermentation produces Cordyceps mycelium；
After fermentation ends, extract mycelium and collect fermentation liquid；Fermentation liquid external heating type Three-effect concentration device is dense
Contracting, each effect vacuum and evaporating temperature: an effect-0.02MPa, 90 DEG C；Two effect-0.04MPa, 80 DEG C；Three
Effect-0.06MPa, 80 DEG C, fermentation liquid is concentrated into relative density 1.31；
The pH of the fermentation liquid after being concentrated by 50L is adjusted to 8.5, and room temperature stands 4h, 10000r/min and is centrifuged 5min
Remove precipitation；
After edible ethanol by 95% pastes, with the flow velocity of 10L/min, the removal precipitation that chamber wall adds 500r/min
Concentrated broth in, in fermentation liquid, concentration of alcohol stops adding when reaching 75%, stops stirring, and 20 DEG C are quiet
Reclaiming supernatant after putting 12h and collect precipitation, precipitation is Cordyceps crude extracellular polysaccharide, obtains crude polysaccharides
4.28kg；
Purifying the crude polysaccharides of gained, dry, pulverize and sieve, must ferment Cordyceps extracellular polysaccharide.
(3) for the method preparing fermentation Cordyceps extracellular polysaccharide granule
Embodiment 5
Taking fermentation Cordyceps extracellular polysaccharide, filler, slow-release material, disintegrating agent, correctives, mixing is all
Sieve after even, take 80 mesh sieve powder；
Binding agent is added content fermentation Cordyceps extracellular polysaccharide, filler, slow-release material, disintegrating agent,
In the wetting agent of correctives and binding agent gross weight 25% mass percent, described wetting agent is dilute with distilled water
Release to concentration be the edible ethanol of 60%；
Wetting agent mixes soft material processed with sieve powder, pelletizes；
60 DEG C are dried 8 hours, sieve, take 20 mesh sieve powder, and must ferment Cordyceps extracellular polysaccharide granule.
Embodiment 6
Taking fermentation Cordyceps extracellular polysaccharide, filler, slow-release material, disintegrating agent, correctives, mixing is all
Sieve after even, take 100 mesh sieve powder；
Binding agent is added content fermentation Cordyceps extracellular polysaccharide, filler, slow-release material, disintegrating agent,
In the wetting agent of correctives and binding agent gross weight 5% mass percent, described wetting agent is to use distilled water diluting
It is the edible ethanol of 80% to concentration；
Wetting agent mixes soft material processed with sieve powder, pelletizes；
60 DEG C are dried 8 hours, sieve, take 40 mesh sieve powder, and must ferment Cordyceps extracellular polysaccharide granule.
The concrete of the present invention manufactures and implementation from the above, really can solve the current artificial fermentation winter
Worm summer grass mode produces the drawback of the fermentation liquor treatment method after Cordyceps mycelium.Owing to fermentation liquid containing
Having a large amount of microorganism, surrounding water and soil can be polluted by direct discharge, need to go out through High Temperature High Pressure
Bacterium processes and just can discharge, and not only wastes ample resources, the energy, human and material resources and financial resources, and right
There is potential safety hazard in surrounding.
Fermentation Cordyceps collection is difficult to and produces the problem of difficulty, and meets the demand in market such that it is able to
Reach the purpose of the present invention.
Claims (10)
1. a fermentation Cordyceps extracellular polysaccharide granule, it is characterised in that its active component used is for sending out
Ferment Cordyceps extracellular polysaccharide, is aided with other pharmaceutic adjuvants, and its raw material components is as follows, respectively organizes percentage by weight
For: fermentation Cordyceps extracellular polysaccharide 20%～55%, filler 20%～60%, slow-release material 5%～20%,
Binding agent 1%～5%, disintegrating agent 0.5%～1%, correctives 0.1%～0.5%.
A kind of fermentation Cordyceps extracellular polysaccharide granule the most according to claim 1, it is characterised in that:
Described filler is selected from the one or many in sucrose or soluble starch or lactose or dextrin or microcrystalline Cellulose
Plant mixture.
A kind of fermentation Cordyceps extracellular polysaccharide granule the most according to claim 1, it is characterised in that:
Described slow-release material is selected from hydroxypropyl methyl cellulose or ethyl cellulose or cellulose acetate or ethyl cellulose
One or more mixture in element aqueous dispersion.
A kind of fermentation Cordyceps extracellular polysaccharide granule the most according to claim 1, it is characterised in that:
Described binding agent is selected from one or more in dextrin or polyvidone or ethyl cellulose or hydroxypropyl cellulose
Mixture.
A kind of fermentation Cordyceps extracellular polysaccharide granule the most according to claim 1, it is characterised in that:
Described disintegrating agent is selected from carboxymethyl starch sodium or microcrystalline Cellulose or cross-linking sodium carboxymethyl cellulose or low replacement
One or more mixture in hydroxypropyl cellulose.
A kind of fermentation Cordyceps extracellular polysaccharide granule the most according to claim 1, it is characterised in that:
Described correctives one or both mixture in stevioside or aspartame.
A kind of fermentation Cordyceps extracellular polysaccharide granule the most according to claim 1, it is characterised in that:
Described fermentation Cordyceps extracellular polysaccharide is prepared by following steps:
(1) utilizing Cordyceps strain, liquid fermentation produces mycelium；
(2), after fermentation ends, extract mycelium and collect fermentation liquid；
(3) fermentation liquid external heating type triple effect concentration tank concentrates, each effect vacuum and evaporating temperature: an effect
-0.02～-0.04MPa, 70～90 DEG C；Two effects-0.04～-0.06MPa, 60～80 DEG C；Triple effect-0.06～-0.08MPa,
50～70 DEG C, fermentation liquid is concentrated into relative density 1.20～1.35；
(4) by 75%～95% edible ethanol with the adherent addition of flow velocity of 1L/min～10L/min
In the concentrated broth of 40r/min～200r/min, in fermentation liquid, concentration of alcohol stops adding when reaching 60%～75%
Enter, stop stirring, collect precipitation after 0～20 DEG C of standing 8～12h and obtain crude polysaccharides；
(5) crude polysaccharides is purified, after dry, pulverize, i.e. obtain fermentation Cordyceps extracellular polysaccharide.
A kind of fermentation Cordyceps extracellular polysaccharide granule the most according to claims 1 to 6, its feature exists
In: its preparation method is as follows: take fermentation Cordyceps extracellular polysaccharide, filler, slow-release material, disintegrating agent,
Correctives, crosses 100 mesh sieves after mix homogeneously, add binding agent in wetting agent, mixes soft material processed with sieve powder,
Pelletizing, 30～80 DEG C are dried 2～12 hours, sieve, take 20～40 mesh sieve powder, Cordyceps born of the same parents of must fermenting
Exo polysaccharides granule.
A kind of fermentation Cordyceps extracellular polysaccharide granule the most according to claim 8, it is characterised in that:
Described wetting agent one or both mixture in distilled water or edible ethanol.
A kind of fermentation Cordyceps extracellular polysaccharide granule the most according to claim 8, its feature exists
In: the consumption of described wetting agent is 5%～25% (weight).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410618258.8A CN105878188A (en) | 2014-11-06 | 2014-11-06 | Fermented cordyceps sinensis extracellular polysaccharide granules and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410618258.8A CN105878188A (en) | 2014-11-06 | 2014-11-06 | Fermented cordyceps sinensis extracellular polysaccharide granules and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105878188A true CN105878188A (en) | 2016-08-24 |
Family
ID=56697890
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410618258.8A Pending CN105878188A (en) | 2014-11-06 | 2014-11-06 | Fermented cordyceps sinensis extracellular polysaccharide granules and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105878188A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110496141A (en) * | 2019-09-10 | 2019-11-26 | 杭州华东医药集团新药研究院有限公司 | A kind of pharmaceutical composition containing cordyceps sinensis |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101700260A (en) * | 2009-11-13 | 2010-05-05 | 秦大伟 | Purslane seed polysaccharide granule preparation and preparation method thereof |
| CN101711770A (en) * | 2009-11-13 | 2010-05-26 | 秦大伟 | Fomitopsis officinalis (Vill. : Fr.) Bond. polysaccharide particle preparation and preparation method thereof |
| CN102511716A (en) * | 2011-12-29 | 2012-06-27 | 天津大学 | Glycyrrhiza polysaccharide granules and preparation method thereof |
| CN103393710A (en) * | 2013-07-26 | 2013-11-20 | 江西济民可信金水宝制药有限公司 | Preparation method and application of ferment cordyceps sinensis powder polysaccharide complex |
-
2014
- 2014-11-06 CN CN201410618258.8A patent/CN105878188A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101700260A (en) * | 2009-11-13 | 2010-05-05 | 秦大伟 | Purslane seed polysaccharide granule preparation and preparation method thereof |
| CN101711770A (en) * | 2009-11-13 | 2010-05-26 | 秦大伟 | Fomitopsis officinalis (Vill. : Fr.) Bond. polysaccharide particle preparation and preparation method thereof |
| CN102511716A (en) * | 2011-12-29 | 2012-06-27 | 天津大学 | Glycyrrhiza polysaccharide granules and preparation method thereof |
| CN103393710A (en) * | 2013-07-26 | 2013-11-20 | 江西济民可信金水宝制药有限公司 | Preparation method and application of ferment cordyceps sinensis powder polysaccharide complex |
Non-Patent Citations (8)
| Title |
|---|
| PO HONG LEUNG等: "Chemical properties and antioxidant activity of exopolysaccharides from mycelial culture of Cordyceps sinensis fungus Cs-HK1", 《FOOD CHEMISTRY》 * |
| 全卫丰，等: "冬虫夏草液体发酵产胞外多糖的研究", 《发酵科技通讯》 * |
| 凌沛学，等: "《中药学概论》", 31 August 2007, 中国轻工业出版社 * |
| 国家食品药品监督管理局执业药师资格认证中心: "《国家执业药师资格考试应试指南中药学专业知识（一）》", 31 January 2014, 中国医药科技出版社 * |
| 崔福德，等: "《药剂学》", 31 August 2011, 人民卫生出版社 * |
| 张日辉: "多糖的提取及优化", 《中国优秀硕士学位论文全文数据库农业科技辑》 * |
| 王安亭，等: "冬虫夏草虫草多糖的分离纯化及含量的测定", 《食用菌》 * |
| 闫景坤，等: "人工培养冬虫夏草胞外多糖的分离纯化研究", 《现代食品科技》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110496141A (en) * | 2019-09-10 | 2019-11-26 | 杭州华东医药集团新药研究院有限公司 | A kind of pharmaceutical composition containing cordyceps sinensis |
| CN110496141B (en) * | 2019-09-10 | 2022-02-25 | 杭州华东医药集团新药研究院有限公司 | A pharmaceutical composition containing Cordyceps |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101897452B (en) | Fermentation production method of grapefruit juice debitterizing enzyme | |
| CN108823261A (en) | A kind of Ultra-low molecular weight Dendrobium officinale polysaccharide and its preparation and application | |
| CN104017098B (en) | A kind of method from mushroom waste mushroom stick extracting directly mushroom active polysaccharide | |
| CN104041893B (en) | A kind of production method of the sea-buckthorn liquid that ferments | |
| CN103751479A (en) | Chinese caterpillar fungus combination containing plants and fungal polysaccharide and preparation method thereof | |
| CN103549394B (en) | A kind of local flavor strengthens edible fungi raw materials processed goods and preparation method thereof | |
| CN105309750A (en) | Comprehensive utilization and production technology of high-content antrodia camphorate fungal active polysaccharide, triterpene fine powder, and byproducts thereof | |
| CN104996729B (en) | Comprehensive recycling technology of radix ophiopogonis decoction dregs | |
| CN101831472B (en) | Method for preparing edible fungi soluble polysaccharide through solid fermentation by taking bean dregs as raw materials | |
| CN101880699A (en) | Method for producing chitooligosaccharides by using microbial fermentation | |
| CN101235025B (en) | Method for preparing theaflavin | |
| CN108586102A (en) | A kind of Se-rich lucid ganoderma culture medium and preparation method thereof | |
| CN102363796A (en) | Method for producing glycyrrhetinic acid through microbial fermentation transformation | |
| CN104877183A (en) | Ganoderma-antradiacomphora polysaccharide and membrane permeation preparation process thereof | |
| CN104844583A (en) | Method for producing puerarin | |
| CN105878188A (en) | Fermented cordyceps sinensis extracellular polysaccharide granules and preparation method thereof | |
| CN104557154A (en) | Method for producing amino acid fertilizer from soybean meal through solid-state fermentation | |
| CN100358921C (en) | Method for producing Konjac Glucomannan in high purity | |
| CN104531804A (en) | Method for production of crystal trehalose and co-production of moisturizing syrup | |
| CN105876405A (en) | Fermented cordyceps sinensis extracellular polysaccharide oral solution and preparation method thereof | |
| CN115595332A (en) | Method for preparing tremella fermentation liquor by combining compound enzyme with microbial fermentation | |
| CN103876014B (en) | Compound phellinus oral liquid and preparation method thereof | |
| KR102062005B1 (en) | Manufacture method of polysaccharide for probiotics cell in mushroom | |
| CN104045723A (en) | Method for extracting tea polysaccharide by biotechnology | |
| CN107348107A (en) | A kind of bran polysaccharide feed addictive with antioxidation activity and preparation method and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160824 |
|
| WD01 | Invention patent application deemed withdrawn after publication |