CN108586102A - A kind of Se-rich lucid ganoderma culture medium and preparation method thereof - Google Patents
A kind of Se-rich lucid ganoderma culture medium and preparation method thereof Download PDFInfo
- Publication number
- CN108586102A CN108586102A CN201810855798.6A CN201810855798A CN108586102A CN 108586102 A CN108586102 A CN 108586102A CN 201810855798 A CN201810855798 A CN 201810855798A CN 108586102 A CN108586102 A CN 108586102A
- Authority
- CN
- China
- Prior art keywords
- parts
- rich
- culture medium
- lucid ganoderma
- selenium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
- C05B1/02—Superphosphates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of Se-rich lucid ganoderma culture mediums and preparation method thereof, are related to ganoderma lucidum technical field, which includes the raw material of following parts by weight：45 60 parts of the dregs of a decoction, 30 60 parts of purple sweet potato stalk, 10 18 parts of konjaku powder, 20 30 parts of Herba Zosterae marinae rich in selenium extracting solution, 15 25 parts of ammonium hydrogen carbonate, 8 12 parts of calcium superphosphate, 6 10 parts of potassium chloride, 36 parts of land plaster, 13 parts of sodium selenite；The preparation method of the culture medium includes the dregs of a decoction and the processing of purple sweet potato stalk, mixing, pack sterilisation step.The Se-rich lucid ganoderma culture medium improves the selenium element content in Medium for Ganoderma lucidum using various ways, and selenium element content in the ganoderma lucidum turned out is made to significantly improve.
Description
Technical field
The invention belongs to ganoderma lucidum technical fields, and in particular to a kind of Se-rich lucid ganoderma culture medium and preparation method thereof.
Background technology
Ganoderma lucidum is the traditional valuable ingredient of traditional Chinese medicine for possessing thousands of years of medicinal histories, has very high medical value, by scientific research machine
The modern pharmacology research of structure many decades confirms that ganoderma lucidum adjusts blood glucose, control blood pressure, auxiliary is swollen for enhancing human immunity
Tumor chemicotherapy, liver protecting promote sleep etc. to all have significant curative effect.
Selenium element is the necessary trace element of human body, and the synthesis of a variety of enzymes and protein, may be used also in selenium element participant's body
With in human body heavy metal and toxic organics combine, and excreted by the metabolic pathway of human body, to protect
Health.Selenium deficiency easily causes heavy metal poisoning, so that human immunological competence is declined and causes a variety of health diseases.
For normal, adult's intake that Chinese NNFA is recommended is micro- gram/day of 50-200, and the selenium that compatriots are daily
Intake only has micro- gram/day of 30-45, is less than required intake, needs to properly increase selenium intake.
The Chinese invention patent application of Publication No. CN107646531A discloses a kind of preparation of Se-rich lucid ganoderma culture medium
Method, the invention include the following steps：(1) selenium salt, sugar, probiotics, amino acid, pure water are mixed into composition zymotic fluid, is sent out
Ferment；(2) zymotic fluid is sterilized and is inactivated, obtain selenium-enriched liquid；(3) by weight by 20-30 parts of cotton seed hulls, 10-20 parts of sawdusts, 8-15
Part wheat bran, 1-6 parts of vitamins, 10-15 parts of potassium dihydrogen phosphates, 5-10 parts of gypsum, 8-16 parts of malt, 2-8 parts of corn flour, corncobs
10-20 parts, 10-20 parts of selenium-enriched liquid mixing, obtain Se-rich lucid ganoderma culture medium.Inorganic selenium has been converted to by probiotics fermention
Machine selenium, is added in the culture medium of ganoderma lucidum, make ganoderma lucidum in growth course by Se accumulation in internal, cultivate the high selenium-rich of selenium content
Ganoderma lucidum.But the selenium element content in the ganoderma lucidum prepared of this method is low.
Notification number is the Chinese invention patent of CN101288374B, discloses a kind of cultivation of selenium-enriched log glossy ganoderma spore powder
Method includes mainly mixing inorganic selenium according to the ratio with lucidum strain and Medium for Ganoderma lucidum, by lucid ganoderma fungus bioconversion, carries
The incubation step of organic selenium content in high conidia powder, selenium-enriched log glossy ganoderma spore powder is as follows：Inorganic selenium sodium selenite is added
Se-rich lucid ganoderma strain is cultivated, sodium selenite is added and prepares Se-rich lucid ganoderma culture medium, accesses lucidum strain, it is inorganic by Ganoderma lucidum mycelium
Selenium approach section wood Internal biological converts, and cultivates Se-rich lucid ganoderma and Se-rich lucid ganoderma conidia powder.The conidia powder Organic Selenium that the present invention cultivates
Content reaches 51mg/kg-130mg/kg, and blood selenium level and immunocompetence significantly improve after human body is taken, and has the good guarantor that cures the disease
Strong effect.But the invention makes ganoderma lucidum convert inorganic selenium to Organic Selenium by sodium selenite and culture medium mixing, due to inorganic
Selenium is toxic, and ganoderma lucidum is low to the transformation efficiency of inorganic selenium, but also inventive method is single, and the selenium element in prepared ganoderma lucidum contains
Amount cannot be met the requirements.
Invention content
The object of the present invention is to provide a kind of Se-rich lucid ganoderma culture medium and preparation method thereof, which uses
Various ways improve the selenium element content in Medium for Ganoderma lucidum, and selenium element content in the ganoderma lucidum turned out is made to significantly improve.
To achieve the above object, the present invention provides the following technical solution：
A kind of Se-rich lucid ganoderma culture medium, includes the raw material of following parts by weight：45-60 parts of the dregs of a decoction, 30-60 parts of purple sweet potato stalk,
10-18 parts of konjaku powder, 20-30 parts of Herba Zosterae marinae rich in selenium extracting solution, 15-25 parts of ammonium hydrogen carbonate, 8-12 parts of calcium superphosphate, potassium chloride 6-
10 parts, 3-6 parts of land plaster, 1-3 parts of sodium selenite.
Preferably, include the raw material of following parts by weight：52.5 parts of the dregs of a decoction, 45 parts of purple sweet potato stalk, 14 parts of konjaku powder, selenium-rich
25 parts of kelp extract, 20 parts of ammonium hydrogen carbonate, 10 parts of calcium superphosphate, 8 parts of potassium chloride, 4.5 parts of land plaster, 2 parts of sodium selenite.
Preferably, the dregs of a decoction are one or more of aloe, pueraria lobata, loguat leaf mixture.
Preferably, the extracting method of the Herba Zosterae marinae rich in selenium extracting solution includes the following steps：
(1) Fresh Laminaria Japonica is put into grinder and is ground, obtain the kelp particle that grain size is 30-100 mesh；
(2) ethanol solution of 5-10 times of its parts by weight is added in kelp particle, it is 160- to wear into grain size using colloid mill
The kelp suspension of 250 mesh, the heat preservation at being 35-50 DEG C in temperature 3-6 hours；
(3) it is cooled to room temperature in kelp suspension, kelp extract is obtained by filtration.
Preferably, the percentage by volume of ethanol solution is 90-99% in the step (2).
Preferably, the preparation method of the konjaku powder is：Fresh konjak is removed the peel, the fritter that length is less than 3cm is cut into,
In addition sodium sorbate solution identical with konjaku parts by weight is put into togerther grinder grinding, the quality percentage of wherein sodium sorbate is dense
Degree is 4-8%, obtains the konjaku slurries that grain size is 120-300 mesh, konjaku slurries are centrifuged, the first filtrate and first are obtained
Ethanol solution identical with the first filter residue parts by weight, the wherein percentage by volume of ethanol solution is added in filter residue in the first filter residue
For 90-99%, 3-5 hours are kept the temperature at being 35-50 DEG C in temperature, the second filtrate is centrifugally separating to obtain, by the first filtrate and second
It is put into drying box after filtrate mixing and is passed through hot gas and is dried to obtain konjaku powder.
Preferably, the hot gas is the hot gas that sulfur burning generates, and dry indoor temperature is 50-80 DEG C.
Preferably, before the drying steps, sulfurous is additionally added in the mixed solution of the first filtrate and the second filtrate
Acid sodium solution.
A kind of preparation method of Se-rich lucid ganoderma culture medium, includes the following steps：
(1) dregs of a decoction and purple sweet potato straw chopping are less than 3cm mixing clasts at length, add water and complex enzyme, is mixing
It is 50-60% that its water content is added water in clast, is accumulated 7-12 days, and the water content of control mixing clast is 50- in banking process
60%, obtain material A；
(2) material A obtained in step (1) is added in mixing vessel, evil spirit is then added in the mixing vessel
Taro fine powder, Herba Zosterae marinae rich in selenium extracting solution, ammonium hydrogen carbonate, calcium superphosphate, potassium chloride, land plaster and sodium selenite, are uniformly mixed
Mixing base-material is obtained, the water content control of mixed resin is 55-60%；
(3) bacterium bag that polypropylene or polyethylene are prepared is added in mixing base-material；
(4) high pressure sterilization is used to bacterium bag, is 2-3Mpa in pressure, sterilizing 3-5 is small under conditions of temperature is 100-110 DEG C
When.
Preferably, complex enzyme is cellulase, protease and pectase in mass ratio 3 in the step (1):2:1 is mixed
It makes, the addition of complex enzyme is to mix the 0.1-0.3% of clast parts by weight.
Beneficial effects of the present invention are that the Se-rich lucid ganoderma culture medium improves the selenium member in Medium for Ganoderma lucidum using various ways
Cellulose content makes selenium element content in the ganoderma lucidum turned out significantly improve；It is specific as follows：
(1) present invention converts inorganic selenium to Organic Selenium with selenium egg by ganoderma lucidum root to the sorption enhanced of sodium selenite
The mode of amino acid and selenoprotein is stored in plant, and it is normal required to supplement that human body can absorb the selenoaminoacid in ganoderma lucidum
Selenium element.
(2) purple sweet potato stalk, konjaku powder and Herba Zosterae marinae rich in selenium extracting solution be also added in the present invention, divided in the life of these materials
Cloth is extensive, convenient for collecting, wherein in purple sweet potato stalk in addition to containing the nutriment in common sweet potato, wherein also containing very
Abundant selenium element, anthocyanidin and ferro element；It is average per selenium of the 100g just containing 350.15 micrograms in konjaku powder, also contain more
Kind vitamin and mineral element；The present invention also extracts Herba Zosterae marinae rich in selenium, can also make kelp clasmatosis, is convenient for selenium element
Absorption.
(3) present invention also added the dregs of a decoction as raw material, and the dregs of a decoction are one or more of aloe, pueraria lobata, loguat leaf,
Wherein containing ingredients such as ganoderma lucidum growth required nitrogen, phosphorus, potassium, and fatal bacterium is free of, content of beary metal is also well below conduct
Fertilizer or the permitted prohibitive content of culture substrate are a kind of good nuisanceless fertilizers；And reed of the present invention
Luxuriant growth, pueraria lobata, the loquat dregs of a decoction, wherein also containing abundant selenium element, plant can directly absorb, and the selenium that can be improved in ganoderma lucidum contains
Amount.
Specific implementation mode
Present invention will be further explained below with reference to specific examples.These embodiments are merely to illustrate the present invention and do not have to
In limiting the scope of the invention.
Embodiment 1
The present embodiment is related to a kind of Se-rich lucid ganoderma culture medium, includes the raw material of following parts by weight：45 parts of the dregs of a decoction, purple sweet potato stalk
30 parts, 10 parts of konjaku powder, 20 parts of Herba Zosterae marinae rich in selenium extracting solution, 15 parts of ammonium hydrogen carbonate, 8 parts of calcium superphosphate, 6 parts of potassium chloride, gypsum
3 parts of powder, 1 part of sodium selenite.
Wherein, the dregs of a decoction are one or more of aloe, pueraria lobata, loguat leaf mixture.
Wherein, the extracting method of the Herba Zosterae marinae rich in selenium extracting solution includes the following steps：
(1) Fresh Laminaria Japonica is put into grinder and is ground, obtain the kelp particle that grain size is 30 mesh；
(2) ethanol solution of 5 times of its parts by weight is added in kelp particle, it is 160 purposes to wear into grain size using colloid mill
Kelp suspension, the heat preservation at being 35 DEG C in temperature 3 hours；Wherein, the percentage by volume of ethanol solution is 90%；
(3) it is cooled to room temperature in kelp suspension, kelp extract is obtained by filtration.
Wherein, the preparation method of the konjaku powder is：Fresh konjak is removed the peel, the fritter that length is 3cm is cut into, in addition
Sodium sorbate solution identical with konjaku parts by weight is put into togerther grinder grinding, and the mass percentage concentration of wherein sodium sorbate is
4%, the konjaku slurries that grain size is 120 mesh are obtained, konjaku slurries are centrifuged, the first filtrate and the first filter residue are obtained, the
Ethanol solution identical with the first filter residue parts by weight is added in one filter residue, the wherein percentage by volume of ethanol solution is 90%,
Temperature is to keep the temperature 3 hours at 35 DEG C, is centrifugally separating to obtain the second filtrate, and drying is put into after the first filtrate and the second filtrate are mixed
It is passed through hot gas in case and is dried to obtain konjaku powder；Wherein, the hot gas is the hot gas that sulfur burning generates, in hothouse
Temperature be 50 DEG C；Wherein, before the drying steps, Asia is additionally added in the mixed solution of the first filtrate and the second filtrate
Metabisulfite solution.
The present embodiment further relates to a kind of preparation method of Se-rich lucid ganoderma culture medium, includes the following steps：
(1) it is 3cm mixing clasts at length by the dregs of a decoction and purple sweet potato straw chopping, adds water and complex enzyme, it is broken mixing
It is 50% that its water content is added water in bits, is accumulated 7 days, and the water content of control mixing clast is 50% in banking process, obtains object
Expect A；Wherein, complex enzyme is cellulase, protease and pectase in mass ratio 3:2:1 is blended, the addition of complex enzyme
Amount is the 0.1% of mixing clast parts by weight；
(2) material A obtained in step (1) is added in mixing vessel, evil spirit is then added in the mixing vessel
Taro fine powder, Herba Zosterae marinae rich in selenium extracting solution, ammonium hydrogen carbonate, calcium superphosphate, potassium chloride, land plaster and sodium selenite, are uniformly mixed
Mixing base-material is obtained, the water content control of mixed resin is 55%；
(3) bacterium bag that polypropylene or polyethylene are prepared is added in mixing base-material；
(4) high pressure sterilization is used to bacterium bag, is 2Mpa in pressure, temperature sterilizes 3 hours under conditions of being 100 DEG C.
Embodiment 2
The present embodiment is related to a kind of Se-rich lucid ganoderma culture medium, includes the raw material of following parts by weight：60 parts of the dregs of a decoction, purple sweet potato stalk
60 parts, 18 parts of konjaku powder, 30 parts of Herba Zosterae marinae rich in selenium extracting solution, 25 parts of ammonium hydrogen carbonate, 12 parts of calcium superphosphate, 10 parts of potassium chloride, stone
6 parts of cream powder, 3 parts of sodium selenite.
Wherein, the dregs of a decoction are one or more of aloe, pueraria lobata, loguat leaf mixture.
Wherein, the extracting method of the Herba Zosterae marinae rich in selenium extracting solution includes the following steps：
(1) Fresh Laminaria Japonica is put into grinder and is ground, obtain the kelp particle that grain size is 100 mesh；
(2) ethanol solution of 10 times of its parts by weight is added in kelp particle, it is 250 purposes to wear into grain size using colloid mill
Kelp suspension, the heat preservation at being 50 DEG C in temperature 6 hours；Wherein, the percentage by volume of ethanol solution is 99%；
(3) it is cooled to room temperature in kelp suspension, kelp extract is obtained by filtration.
Wherein, the preparation method of the konjaku powder is：Fresh konjak is removed the peel, the fritter that length is 3cm is cut into, in addition
Sodium sorbate solution identical with konjaku parts by weight is put into togerther grinder grinding, and the mass percentage concentration of wherein sodium sorbate is
8%, the konjaku slurries that grain size is 300 mesh are obtained, konjaku slurries are centrifuged, the first filtrate and the first filter residue are obtained, the
Ethanol solution identical with the first filter residue parts by weight is added in one filter residue, the wherein percentage by volume of ethanol solution is 99%,
Temperature is to keep the temperature 5 hours at 50 DEG C, is centrifugally separating to obtain the second filtrate, and drying is put into after the first filtrate and the second filtrate are mixed
It is passed through hot gas in case and is dried to obtain konjaku powder；Wherein, the hot gas is the hot gas that sulfur burning generates, in hothouse
Temperature be 80 DEG C；Wherein, before the drying steps, Asia is additionally added in the mixed solution of the first filtrate and the second filtrate
Metabisulfite solution.
The present embodiment further relates to a kind of preparation method of Se-rich lucid ganoderma culture medium, includes the following steps：
(1) it is 3cm mixing clasts at length by the dregs of a decoction and purple sweet potato straw chopping, adds water and complex enzyme, it is broken mixing
It is 60% that its water content is added water in bits, is accumulated 12 days, and the water content of control mixing clast is 60% in banking process, obtains object
Expect A；Wherein, complex enzyme is cellulase, protease and pectase in mass ratio 3:2:1 is blended, the addition of complex enzyme
Amount is the 0.3% of mixing clast parts by weight；
(2) material A obtained in step (1) is added in mixing vessel, evil spirit is then added in the mixing vessel
Taro fine powder, Herba Zosterae marinae rich in selenium extracting solution, ammonium hydrogen carbonate, calcium superphosphate, potassium chloride, land plaster and sodium selenite, are uniformly mixed
Mixing base-material is obtained, the water content control of mixed resin is 60%；
(3) bacterium bag that polypropylene or polyethylene are prepared is added in mixing base-material；
(4) high pressure sterilization is used to bacterium bag, is 3Mpa in pressure, temperature sterilizes 5 hours under conditions of being 110 DEG C.
Embodiment 3
The present embodiment is related to a kind of Se-rich lucid ganoderma culture medium, includes the raw material of following parts by weight：50 parts of the dregs of a decoction, purple sweet potato stalk
35 parts, 14 parts of konjaku powder, 25 parts of Herba Zosterae marinae rich in selenium extracting solution, 20 parts of ammonium hydrogen carbonate, 8 parts of calcium superphosphate, 8 parts of potassium chloride, gypsum
4 parts of powder, 2 parts of sodium selenite.
Wherein, the dregs of a decoction are one or more of aloe, pueraria lobata, loguat leaf mixture.
Wherein, the extracting method of the Herba Zosterae marinae rich in selenium extracting solution includes the following steps：
(1) Fresh Laminaria Japonica is put into grinder and is ground, obtain the kelp particle that grain size is 60 mesh；
(2) ethanol solution of 6 times of its parts by weight is added in kelp particle, it is 180 purposes to wear into grain size using colloid mill
Kelp suspension, the heat preservation at being 40 DEG C in temperature 5 hours；Wherein, the percentage by volume of ethanol solution is 93%；
(3) it is cooled to room temperature in kelp suspension, kelp extract is obtained by filtration.
Wherein, the preparation method of the konjaku powder is：Fresh konjak is removed the peel, the fritter that length is 3cm is cut into, in addition
Sodium sorbate solution identical with konjaku parts by weight is put into togerther grinder grinding, and the mass percentage concentration of wherein sodium sorbate is
6%, the konjaku slurries that grain size is 160 mesh are obtained, konjaku slurries are centrifuged, the first filtrate and the first filter residue are obtained, the
Ethanol solution identical with the first filter residue parts by weight is added in one filter residue, the wherein percentage by volume of ethanol solution is 97%,
Temperature is to keep the temperature 4 hours at 40 DEG C, is centrifugally separating to obtain the second filtrate, and drying is put into after the first filtrate and the second filtrate are mixed
It is passed through hot gas in case and is dried to obtain konjaku powder；Wherein, the hot gas is the hot gas that sulfur burning generates, in hothouse
Temperature be 55 DEG C；Wherein, before the drying steps, Asia is additionally added in the mixed solution of the first filtrate and the second filtrate
Metabisulfite solution.
The present embodiment further relates to a kind of preparation method of Se-rich lucid ganoderma culture medium, includes the following steps：
(1) it is 3cm mixing clasts at length by the dregs of a decoction and purple sweet potato straw chopping, adds water and complex enzyme, it is broken mixing
It is 53% that its water content is added water in bits, is accumulated 8 days, and the water content of control mixing clast is 56% in banking process, obtains object
Expect A；Wherein, complex enzyme is cellulase, protease and pectase in mass ratio 3:2:1 is blended, the addition of complex enzyme
Amount is the 0.2% of mixing clast parts by weight；
(2) material A obtained in step (1) is added in mixing vessel, evil spirit is then added in the mixing vessel
Taro fine powder, Herba Zosterae marinae rich in selenium extracting solution, ammonium hydrogen carbonate, calcium superphosphate, potassium chloride, land plaster and sodium selenite, are uniformly mixed
Mixing base-material is obtained, the water content control of mixed resin is 58%；
(3) bacterium bag that polypropylene or polyethylene are prepared is added in mixing base-material；
(4) high pressure sterilization is used to bacterium bag, is 2Mpa in pressure, temperature sterilizes 4 hours under conditions of being 100 DEG C.
Embodiment 4
The present embodiment is related to a kind of Se-rich lucid ganoderma culture medium, includes the raw material of following parts by weight：50 parts of the dregs of a decoction, purple sweet potato stalk
40 parts, 13 parts of konjaku powder, 23 parts of Herba Zosterae marinae rich in selenium extracting solution, 20 parts of ammonium hydrogen carbonate, 10 parts of calcium superphosphate, 8 parts of potassium chloride, gypsum
5 parts of powder, 2 parts of sodium selenite.
Wherein, the dregs of a decoction are one or more of aloe, pueraria lobata, loguat leaf mixture.
Wherein, the extracting method of the Herba Zosterae marinae rich in selenium extracting solution includes the following steps：
(1) Fresh Laminaria Japonica is put into grinder and is ground, obtain the kelp particle that grain size is 60 mesh；
(2) ethanol solution of 7 times of its parts by weight is added in kelp particle, it is 180 purposes to wear into grain size using colloid mill
Kelp suspension, the heat preservation at being 45 DEG C in temperature 4 hours；Wherein, the percentage by volume of ethanol solution is 96%；
(3) it is cooled to room temperature in kelp suspension, kelp extract is obtained by filtration.
Wherein, the preparation method of the konjaku powder is：Fresh konjak is removed the peel, the fritter that length is 3cm is cut into, in addition
Sodium sorbate solution identical with konjaku parts by weight is put into togerther grinder grinding, and the mass percentage concentration of wherein sodium sorbate is
4%, the konjaku slurries that grain size is 210 mesh are obtained, konjaku slurries are centrifuged, the first filtrate and the first filter residue are obtained, the
Ethanol solution identical with the first filter residue parts by weight is added in one filter residue, the wherein percentage by volume of ethanol solution is 91%,
Temperature is to keep the temperature 4 hours at 39 DEG C, is centrifugally separating to obtain the second filtrate, and drying is put into after the first filtrate and the second filtrate are mixed
It is passed through hot gas in case and is dried to obtain konjaku powder；Wherein, the hot gas is the hot gas that sulfur burning generates, in hothouse
Temperature be 58 DEG C；Wherein, before the drying steps, Asia is additionally added in the mixed solution of the first filtrate and the second filtrate
Metabisulfite solution.
The present embodiment further relates to a kind of preparation method of Se-rich lucid ganoderma culture medium, includes the following steps：
(1) it is 3cm mixing clasts at length by the dregs of a decoction and purple sweet potato straw chopping, adds water and complex enzyme, it is broken mixing
It is 54% that its water content is added water in bits, is accumulated 9 days, and the water content of control mixing clast is 50% in banking process, obtains object
Expect A；Wherein, complex enzyme is cellulase, protease and pectase in mass ratio 3:2:1 is blended, the addition of complex enzyme
Amount is the 0.1% of mixing clast parts by weight；
(2) material A obtained in step (1) is added in mixing vessel, evil spirit is then added in the mixing vessel
Taro fine powder, Herba Zosterae marinae rich in selenium extracting solution, ammonium hydrogen carbonate, calcium superphosphate, potassium chloride, land plaster and sodium selenite, are uniformly mixed
Mixing base-material is obtained, the water content control of mixed resin is 55%；
(3) bacterium bag that polypropylene or polyethylene are prepared is added in mixing base-material；
(4) high pressure sterilization is used to bacterium bag, is 3Mpa in pressure, temperature sterilizes 3 hours under conditions of being 110 DEG C.
Embodiment 5
The present embodiment is related to a kind of Se-rich lucid ganoderma culture medium, includes the raw material of following parts by weight：52.5 parts of the dregs of a decoction, purple sweet potato straw
45 parts of stalk, 14 parts of konjaku powder, 25 parts of Herba Zosterae marinae rich in selenium extracting solution, 20 parts of ammonium hydrogen carbonate, 10 parts of calcium superphosphate, 8 parts of potassium chloride, stone
4.5 parts of cream powder, 2 parts of sodium selenite.
Wherein, the dregs of a decoction are one or more of aloe, pueraria lobata, loguat leaf mixture.
Wherein, the extracting method of the Herba Zosterae marinae rich in selenium extracting solution includes the following steps：
(1) Fresh Laminaria Japonica is put into grinder and is ground, obtain the kelp particle that grain size is 65 mesh；
(2) ethanol solution of 7.5 times of its parts by weight is added in kelp particle, it is 205 mesh to wear into grain size using colloid mill
Kelp suspension, the heat preservation 4.5 hours in the case where temperature is 42.5 DEG C；Wherein, the percentage by volume of ethanol solution is 94.5%；
(3) it is cooled to room temperature in kelp suspension, kelp extract is obtained by filtration.
Wherein, the preparation method of the konjaku powder is：Fresh konjak is removed the peel, the fritter that length is 3cm is cut into, in addition
Sodium sorbate solution identical with konjaku parts by weight is put into togerther grinder grinding, and the mass percentage concentration of wherein sodium sorbate is
6%, the konjaku slurries that grain size is 210 mesh are obtained, konjaku slurries are centrifuged, the first filtrate and the first filter residue are obtained, the
Ethanol solution identical with the first filter residue parts by weight is added in one filter residue, the wherein percentage by volume of ethanol solution is 94.5%,
34 hours are kept the temperature at being 42.5 DEG C in temperature, is centrifugally separating to obtain the second filtrate, is put after the first filtrate and the second filtrate are mixed
Enter to be passed through hot gas in drying box and is dried to obtain konjaku powder；Wherein, the hot gas is the hot gas that sulfur burning generates, and is done
Dry indoor temperature is 65 DEG C；Wherein, before the drying steps, in the mixed solution of the first filtrate and the second filtrate also
Sodium sulfite solution is added.
The present embodiment further relates to a kind of preparation method of Se-rich lucid ganoderma culture medium, includes the following steps：
(1) it is 3cm mixing clasts at length by the dregs of a decoction and purple sweet potato straw chopping, adds water and complex enzyme, it is broken mixing
It is 55% that its water content is added water in bits, is accumulated 9.5 days, and the water content of control mixing clast is 55% in banking process, is obtained
Material A；Wherein, complex enzyme is cellulase, protease and pectase in mass ratio 3:2:1 is blended, and complex enzyme adds
It is mix clast parts by weight 0.2% to enter amount；
(2) material A obtained in step (1) is added in mixing vessel, evil spirit is then added in the mixing vessel
Taro fine powder, Herba Zosterae marinae rich in selenium extracting solution, ammonium hydrogen carbonate, calcium superphosphate, potassium chloride, land plaster and sodium selenite, are uniformly mixed
Mixing base-material is obtained, the water content control of mixed resin is 57.5%；
(3) bacterium bag that polypropylene or polyethylene are prepared is added in mixing base-material；
(4) high pressure sterilization is used to bacterium bag, is 2.5Mpa in pressure, temperature sterilizes 3.5 hours under conditions of being 105 DEG C.
Comparative example 1
Using Medium for Ganoderma lucidum made from Chinese invention patent application (Publication No. CN107646531A).
Comparative example 2
The preparation method of Herba Zosterae marinae rich in selenium extracting solution is：Herba Zosterae marinae rich in selenium and mass fraction are mixed for the ethanol solution of 90-99%
It closes, reflux purification 3-6 hours, the wherein mass parts of ethanol solution are 5-10 times of Herba Zosterae marinae rich in selenium parts by weight, and selenium-rich is obtained by filtration
Kelp extract；Other raw materials, content and step are consistent with embodiment.
Comparative example 3
In addition to no Herba Zosterae marinae rich in selenium extracting solution, other raw materials, content are consistent with step and embodiment.
Comparative example 4
In addition to no purple sweet potato stalk, other raw materials, content and step are consistent with embodiment.
Comparative example 5
Se-rich lucid ganoderma culture medium made from the prior art.
Ganoderma lucidum is cultivated using above-described embodiment 1-5 and comparative example 1-5 the Se-rich lucid ganoderma culture medium prepared；According to
National standard is detected the Se content in the lucidum spore powder stage and fructification stage of medium culture, and detection method is
Inductance coupling ion atoms emission spectrometry；What spore powder yield and fruiting body yield were acquired for the culture medium per 10kg；It obtains
The results are shown in Table 1：
Table 1
As can be seen from the table, it acts synergistically, reaches jointly desired by the present invention between each each raw material of step in the present invention
Effect, Se-rich lucid ganoderma culture medium degradation obtained can all be made by lacking step, raw material or change raw material, in conjunction with comparison
Example 3 and comparative example 4, it can be seen that lack the selenium element content that Herba Zosterae marinae rich in selenium extracting solution and purple sweet potato stalk will make the ganoderma lucidum turned out
With ganoderma lucidum yield by different degrees of weakening, in conjunction with comparative example 2 as can be seen that the preparation of the Herba Zosterae marinae rich in selenium extracting solution of the present invention
Method compared with the existing technology in extracting mode it is creative, lacking Herba Zosterae marinae rich in selenium extracting solution can make the content of selenium element aobvious
It writes and declines.
As can be seen from the table, embodiment 5 is highly preferred embodiment of the present invention, is trained using Se-rich lucid ganoderma culture medium of the present invention
The ganoderma lucidum raised compared with the existing technology for be obtained for prodigious improvement, conidia powder organic selenium content maximum has reached
130mg/Kg improves 78% compared with the existing technology；Fructification organic selenium content maximum has reached 230 compared with the existing technology
Improve 82.5%；And spore powder yield is up to 351g and improves 41.5% compared with the existing technology；And fruiting body yield is most
Have reached 332g improves 46.25% compared with the existing technology greatly.
Specific embodiments of the present invention are described above.It is to be appreciated that the invention is not limited in above-mentioned
Particular implementation, those skilled in the art can make various deformations or amendments within the scope of the claims, this not shadow
Ring the substantive content of the present invention.
Claims (10)
1. a kind of Se-rich lucid ganoderma culture medium, which is characterized in that include the raw material of following parts by weight：45-60 parts of the dregs of a decoction, purple sweet potato stalk
30-60 parts, 10-18 parts of konjaku powder, 20-30 parts of Herba Zosterae marinae rich in selenium extracting solution, 15-25 parts of ammonium hydrogen carbonate, 8-12 parts of calcium superphosphate,
6-10 parts of potassium chloride, 3-6 parts of land plaster, 1-3 parts of sodium selenite.
2. Se-rich lucid ganoderma culture medium according to claim 1, which is characterized in that include the raw material of following parts by weight：The dregs of a decoction
52.5 parts, 45 parts of purple sweet potato stalk, 14 parts of konjaku powder, 25 parts of Herba Zosterae marinae rich in selenium extracting solution, 20 parts of ammonium hydrogen carbonate, calcium superphosphate 10
Part, 8 parts of potassium chloride, 4.5 parts of land plaster, 2 parts of sodium selenite.
3. Se-rich lucid ganoderma culture medium according to claim 1, which is characterized in that the dregs of a decoction are aloe, pueraria lobata, loguat leaf
One or more of mixture.
4. Se-rich lucid ganoderma culture medium according to claim 1, which is characterized in that the extraction side of the Herba Zosterae marinae rich in selenium extracting solution
Method includes the following steps：
(1) Fresh Laminaria Japonica is put into grinder and is ground, obtain the kelp particle that grain size is 30-100 mesh；
(2) ethanol solution of 5-10 times of its parts by weight is added in kelp particle, it is 160-250 mesh to wear into grain size using colloid mill
Kelp suspension, heat preservation 3-6 hours in the case where temperature is 35-50 DEG C；
(3) it is cooled to room temperature in kelp suspension, kelp extract is obtained by filtration.
5. Se-rich lucid ganoderma culture medium according to claim 4, which is characterized in that the body of ethanol solution in the step (2)
Product percentage is 90-99%.
6. Se-rich lucid ganoderma culture medium according to claim 1, which is characterized in that the preparation method of the konjaku powder is：
Fresh konjak is removed the peel, the fritter that length is less than 3cm is cut into, sodium sorbate solution identical with konjaku parts by weight is added and puts together
Entering grinder grinding, the wherein mass percentage concentration of sodium sorbate is 4-8%, obtains the konjaku slurries that grain size is 120-300 mesh,
Konjaku slurries are centrifuged, the first filtrate and the first filter residue are obtained, are added and the first filter residue parts by weight phase in the first filter residue
Same ethanol solution, the wherein percentage by volume of ethanol solution are 90-99%, and 3-5 hours are kept the temperature at being 35-50 DEG C in temperature,
Be centrifugally separating to obtain the second filtrate, will the first filtrate and the second filtrate mix after be put into drying box and be passed through hot gas and be dried to obtain
Konjaku powder.
7. Se-rich lucid ganoderma culture medium according to claim 6, which is characterized in that the hot gas is what sulfur burning generated
Hot gas, dry indoor temperature are 50-80 DEG C.
8. Se-rich lucid ganoderma culture medium according to claim 6, which is characterized in that before the drying steps, first
It is additionally added sodium sulfite solution in the mixed solution of filtrate and the second filtrate.
9. the preparation method of the Se-rich lucid ganoderma culture medium described in a kind of any one of claim 1 to 8, which is characterized in that packet
Include following steps：
(1) dregs of a decoction and purple sweet potato straw chopping are less than 3cm mixing clasts at length, add water and complex enzyme, in mixing clast
In to add water to its water content be 50-60%, accumulate 7-12 days, the water content of control mixing clast is 50-60% in banking process,
Obtain material A；
(2) material A obtained in step (1) is added in mixing vessel, konjaku essence then is added in the mixing vessel
Powder, Herba Zosterae marinae rich in selenium extracting solution, ammonium hydrogen carbonate, calcium superphosphate, potassium chloride, land plaster and sodium selenite, are uniformly mixed to obtain
The water content control of mixing base-material, mixed resin is 55-60%；
(3) bacterium bag that polypropylene or polyethylene are prepared is added in mixing base-material；
(4) high pressure sterilization is used to bacterium bag, is 2-3Mpa in pressure, temperature sterilizes 3-5 hours under conditions of being 100-110 DEG C.
10. the preparation method of Se-rich lucid ganoderma culture medium according to claim 9, which is characterized in that multiple in the step (1)
Synthase is cellulase, protease and pectase in mass ratio 3:2:1 is blended, and the addition of complex enzyme is mixing clast
The 0.1-0.3% of parts by weight.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810855798.6A CN108586102A (en) | 2018-07-31 | 2018-07-31 | A kind of Se-rich lucid ganoderma culture medium and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810855798.6A CN108586102A (en) | 2018-07-31 | 2018-07-31 | A kind of Se-rich lucid ganoderma culture medium and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108586102A true CN108586102A (en) | 2018-09-28 |
Family
ID=63618444
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810855798.6A Withdrawn CN108586102A (en) | 2018-07-31 | 2018-07-31 | A kind of Se-rich lucid ganoderma culture medium and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108586102A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110122163A (en) * | 2019-05-16 | 2019-08-16 | 安徽吾悦农业科技有限公司 | A kind of ganoderma lucidum cultivation method improving biological transformation ratio |
| CN111226697A (en) * | 2020-02-25 | 2020-06-05 | 张旺凡 | Ganoderma lucidum mycelium powder rich in multiple trace elements and preparation method thereof |
| CN111657052A (en) * | 2020-05-28 | 2020-09-15 | 大连富森食用菌研究所 | Selenium-rich culture medium for liquid strains of edible fungi and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107853077A (en) * | 2017-12-05 | 2018-03-30 | 罗成喜 | A kind of solid Se-rich lucid ganoderma culture medium and its method for producing ganoderma lucidum |
-
2018
- 2018-07-31 CN CN201810855798.6A patent/CN108586102A/en not_active Withdrawn
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107853077A (en) * | 2017-12-05 | 2018-03-30 | 罗成喜 | A kind of solid Se-rich lucid ganoderma culture medium and its method for producing ganoderma lucidum |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110122163A (en) * | 2019-05-16 | 2019-08-16 | 安徽吾悦农业科技有限公司 | A kind of ganoderma lucidum cultivation method improving biological transformation ratio |
| CN111226697A (en) * | 2020-02-25 | 2020-06-05 | 张旺凡 | Ganoderma lucidum mycelium powder rich in multiple trace elements and preparation method thereof |
| CN111657052A (en) * | 2020-05-28 | 2020-09-15 | 大连富森食用菌研究所 | Selenium-rich culture medium for liquid strains of edible fungi and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104473145B (en) | A kind of Antrodia camphorata submerged fermentation compound product and preparation method thereof | |
| CN100494341C (en) | Huishuhua fermentation preparation process using Chinese herb medicine as medium | |
| CN103420731B (en) | Compatibility of ganoderma lucidum cultivation material and preparation method of ganoderma lucidum cultivation material | |
| CN100399877C (en) | Cultivation of selenium enriched morin and method of extracting morin selenium polysacharide | |
| CN101948355A (en) | Preparation method of culture medium of edible fungi | |
| CN108586102A (en) | A kind of Se-rich lucid ganoderma culture medium and preparation method thereof | |
| CN103553834A (en) | Method for preparing tremella cultivation material from tea cattail and tea seed shells | |
| CN104068379A (en) | Health-care oral solution | |
| CN105146103A (en) | Feed additive | |
| CN104402617A (en) | Pleurotus eryngii cultivation material with use of garlic straw and pleurotus eryngii production method | |
| CN102870594B (en) | Method for culturing hericium erinaceus through tobacco stem facility | |
| CN103932181B (en) | A kind ofly to release the pressure and the functional food of Improving memory and two-step fermentation preparation method thereof | |
| CN103976351A (en) | Health food capable of enhancing immunity and improving sleep and two-step fermentation preparation method thereof | |
| CN110923281A (en) | Edible fungus polysaccharide extraction method, edible fungus polysaccharide and edible fungus beverage | |
| CN104000192A (en) | Functional food for relieving stress and regulating intestines and stomach and two-step fermentation preparation method of functional food | |
| CN104187587A (en) | Edible fungus healthcare oral liquid and preparing method thereof | |
| CN106187472A (en) | A kind of black fungus rich in selenium compost | |
| CN108934773A (en) | A kind of Medium for Ganoderma lucidum and preparation method thereof | |
| CN112715278B (en) | Chinese medicinal Coriolus versicolor and its cultivation method | |
| CN113277899A (en) | Preparation method of leaf fertilizer rich in selenium and amino acid | |
| KR20110067813A (en) | Mixed tea using leaf, root bark and fruit of mulberry tree | |
| CN104970354B (en) | A kind of reducing blood lipid and the functional food and its two-step fermentation preparation method of hypotensive | |
| CN107500912A (en) | A kind of cultivation matrix for improving mushroom medical value and preparation method thereof | |
| CN107673888A (en) | Plant sweet potato new and effective bacterial manure and preparation method thereof | |
| CN113957109B (en) | Industrial green production process of polystictus glycopeptide |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20180928 |