CN105309750A - Comprehensive utilization and production technology of high-content antrodia camphorate fungal active polysaccharide, triterpene fine powder, and byproducts thereof - Google Patents
Comprehensive utilization and production technology of high-content antrodia camphorate fungal active polysaccharide, triterpene fine powder, and byproducts thereof Download PDFInfo
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- CN105309750A CN105309750A CN201410339121.9A CN201410339121A CN105309750A CN 105309750 A CN105309750 A CN 105309750A CN 201410339121 A CN201410339121 A CN 201410339121A CN 105309750 A CN105309750 A CN 105309750A
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Abstract
The invention discloses a comprehensive utilization and production technology of high-content antrodia camphorate fungal active polysaccharide, triterpene fine powder, and byproducts thereof, belongs to the technical field of biological engineering, and especially relates to a technology using a biological fermentation technology to produce antrodia camphorate fungal active polysaccharide and triterpene. The technology is characterized in that corn is taken as the raw material, solid corn particles are taken as the culture medium, wild antrodia camphorate strains are inoculated on the culture medium and cultured; under specified conditions, antrodia camphorate mycelium can be obtained, the antrodia camphorate mycelium is subjected to extraction, condensation, and alcohol precipitation in high purity grain wine so as to obtain antrodia camphorate fungal active polysaccharide and triterpene fine powder; and the products in each step can be made into various products containing effective components such as livestock and poultry feed additive, antrodia camphorate beverage, cosmetics, antrodia camphorate wine, and the like. No waste is generated during the whole production process. According to the production technology , the solid particles are fermented, the technology is stable, the yield is high, the content of antrodia camphorate polysaccharide reaches 80%, the content of triterpene is 30%, the product quality is high, and a prominent economic benefit is generated.
Description
Technical field
The comprehensive utilization process of the present invention's a kind of high-load Antrodia camphorata fungi polysaccharide and triterpene fine powder and byproduct thereof, belongs to technical field of bioengineering, and particularly biofermentation technique produces the technique of Antrodia camphorata fungi activity polysaccharide and triterpene.
Background technology
Since ancient times, Antrodia camphorata is as the jewellery of Traditional Chinese Medicine, be endowed inscrutable mystery flavour, along with the research and development of modern medicine, people have fully realized nucleus---polysaccharide and the triterpene of Antrodia camphorata as medicine, health care, and Antrodia camphorata can be adopted for raw material and obtain them with modern biotechnology, the process obtaining polysaccharide and triterpene in recent years emerges in an endless stream, but substantially can be divided into following two classes:
1, from Antrodia camphorata fructification, water extraction process produces Antrodia camphorata fungi activity polysaccharide and triterpene
At the beginning of the eighties, Taiwan Li Jin wood, Wang Zhijie separation and Extraction water-soluble Antrodia camphorata fungi activity polysaccharide and triterpene from Antrodia camphorata fructification, in the product of extraction, polyoses content is 50%, and triterpene content is about 10%.
Conventional method is that Antrodia camphorata fructification raw material is broken into bulk, adds water, and decoct under normal pressure and extract filtrate, its residue carries out second time again and decocts extraction, forms product by concentrated for filtrate.
2, Antrodia camphorata fungi activity polysaccharide and triterpene is produced with bioengineering fermentation technique
What generally adopt now is that liquid fermentation technology produces Antrodia camphorata fungi activity polysaccharide and triterpene, adopt the culture medium aqueous solution of conventional carbonaceous sources, nitrogenous source, inorganic salts and vitamin, by Antrodia camphorata strain inoculation in fluid nutrient medium, again under certain condition, after liquid fermentation, from zymotic fluid and mycelium, obtain Antrodia camphorata fungi activity polysaccharide and triterpene.
Summary of the invention
The object of the invention is by selecting pollution-free ecological ambient growth, be rich in the natural material of rare element germanium and selenium especially, with solid forms culture medium, inoculation wild Antrodia camphorata bacterial classification, cultivate Antrodia camphorata mycelium, by its comprehensive utilization process, obtain high-load Antrodia camphorata fungi activity polysaccharide and triterpene and byproduct thereof, and technique overall process is without discarded object output.
The present invention seeks to be achieved through the following technical solutions: namely select the corn being rich in rare element germanium and selenium of pollution-free mountain area soil and weather ambient growth to be base-material, add appropriate conventional containing K+, Ca++, Zn++, the inorganic salts of Mg++ amount are mixed with corn particle culture medium, the bacterial classification that the bacterial strain inoculating the wild Antrodia camphorata of wild high-quality or Antrodia camphorata is cultivated, bacteriumization is cultivated again under rated condition, obtain high-quality Antrodia camphorata mycelium, with in the whole technical process of this Antrodia camphorata mycelium production and processing Antrodia camphorata fungi activity polysaccharide and triterpene fine powder major product, the output object fully utilizing each processing step makes the byproduct becoming Antrodia camphorata fungi activity polysaccharide and triterpene, whole technical process is without any discarded object output.
1, the comprehensive utilization process of a kind of high-load Antrodia camphorata fungi activity polysaccharide and triterpene fine powder and byproduct thereof, adopt solid shaped bodies culture medium, through inoculation Antrodia camphorata bacterial classification, prepare Antrodia camphorata mycelium and produce Antrodia camphorata fungi activity polysaccharide and triterpene and byproduct thereof, it is characterized in that comprising following processing step:
One, solid shaped bodies particulate media is prepared
Take corn as raw material, through the permeable immersion of normal temperature, steam removes the pretreated loose corn particle base-material of mould, and add the appropriate conventional inorganic salts containing K+, Ca++, Zn++, Mg++, Homogeneous phase mixing makes faintly acid solid shaped bodies culture medium;
Two, mycelium is cultivated
Above-mentioned culture medium is placed in sealed glass container, after high pressure steam sterilization process, in gnotobasis access Antrodia camphorata bacterial classification, sealing, bacteriumization is cultivated in culturing room's environment of uniform temperature, damp condition, go out plentiful Antrodia camphorata mycelium in the superficial growth of solid shaped bodies particulate media, take out mycelium, fresh goods namely with or oven dry to do dry product for subsequent use;
Three, mycelium, lixiviate Antrodia camphorata fungi activity polysaccharide and triterpene is dissolved
With 100 of appropriate ratio DEG C of alkalescent deionized waters, dissolve mycelium, each lixiviate about 50 minutes, at least lixiviate filters twice, extracts Antrodia camphorata fungi activity polysaccharide and triterpene, and reclaims filter residue;
Four, concentrated, alcohol is analysed, output Antrodia camphorata fungi activity polysaccharide and triterpene major product, reclaim byproduct merging filtrate, in 55-70 DEG C, under 0.07-0.09MPa pressure, Vacuum Concentration, reclaim the deionized water of concentration process evaporation, and in this concentrate, add 3-4 doubly carry out alcohol to the high-purity grain wine of weight of material and analyse, precipitation Antrodia camphorata fungi activity polysaccharide and triterpene, precipitation and separation, reclaim the grain wine liquid material after precipitation and separation, isolated precipitated product is dry, be processed into the Antrodia camphorata fungi activity polysaccharide processing step relevant with processing with triterpene fine powder and reclaim the feed addictive that output object is Antrodia camphorata fungi activity polysaccharide and triterpene active ingredient, Antrodia camphorata dew and various Antrodia camphorata Wine drink.
2, according to comprehensive utilization process of the present invention, it is characterized in that the maize raw material adopted comes from pollution-free ecological environment, be naturally rich in rare element germanium and selenium, be permeablely dipped to moisture 50-60%.
3, according to comprehensive utilization process of the present invention, it is characterized in that Mycelium culture room environmental temperature about 25, relative humidity 65-70%,
4, according to comprehensive utilization process of the present invention, it is characterized in that the deionized water of lixiviate Antrodia camphorata active polysaccharide and triterpene and mycelium fresh goods ratio (W/W) are 3-3.5: 1, is 6-6.5: 1 with mycelium dry product ratio (W/W).
5, according to comprehensive utilization process of the present invention, it is characterized in that the filter vacuum merged is concentrated into proportion 1.1-1.3.
6, according to the comprehensive utilization process of invention, it is characterized in that the high-purity grain wine that alcohol analyses Antrodia camphorata active polysaccharide and triterpene is 80 DEG C.
The present invention has outstanding technological progress:
1, comprehensive utilization process of the present invention, adopt solid shaped bodies particle to make culture medium zymotechnique, process stabilizing, productive rate is high, Antrodia camphorata active polysaccharide and triterpene content high, at least polysaccharide reaches 80%, triterpene 30%, and product quality is excellent, has significant economic benefit.
2, comprehensive utilization process of the present invention is the ecological process pattern of enclosed raw material-product-raw material-byproduct, except major product Antrodia camphorata active polysaccharide and triterpene, reclaim relevant output object in its technical process: reclaim filter residue and be processed into poultry and livestock feed additive simultaneously; The deionized water reclaiming concentration process evaporation is prepared high-purity Antrodia camphorata dew health beverages and makes face nourishing skin care products raw material; The grain wine liquid material after precipitation analysed by recovery alcohol, prepares the various Antrodia camphorata Wine drink containing Antrodia camphorata active polysaccharide and triterpene effective ingredient.Whole technical process does not produce any discarded object, does not produce any pollution to soil, water body and air, has extremely significantly social benefit.
3, the culture medium raw material that adopts of comprehensive utilization process of the present invention and bacterial classification all come from natural green crop without the growth of dye ecological environment and bacterial strain, and whole production process does not add any chemicals pigment and additive, ensure that the natural, green of product and high-quality.
Now further illustrate the present invention in conjunction with the embodiments.
Embodiment one
Select west place in Hubei to bestow favour corn, impurity elimination, be dipped to corn moisture 45%, for subsequent use after steam sterilizing process.Get above-mentioned corn particle 2500 grams, add 30 grams, edible plaster powder, add A, R level magnesium sulfate, zinc sulfate and each 12 grams of potassium dihydrogen phosphate, abundant uniform mixing, PH is adjusted to be 6.5, being respectively charged into 5 volumes is in the vial of 500ml, sealing is placed in pressure cooker, 0.147MPa sterilized under pressure 1.5 hours, pressurize 3 hours, treat Pressure Drop to zero, temperature is down to 25 DEG C, in disinfecting action district, by the preprepared wild Antrodia camphorata fungi strain of edible fungi inoculation method access, temperature 25 DEG C is given in sealing, humidity 70%, cultivate 30 days without in the culturing room of direct sunlight, the mycelium taken out, dry in the air dry oven of 60 DEG C ± 1 DEG C, obtain mycelium dry product 1056 grams.
Extract in bucket at stainless steel and add deionized water 6 kilograms, PH is adjusted to be 7.8, be heated to 100 DEG C take 1 kilogram of Antrodia camphorata mycelia dry product and be placed in the stainless steel well-bucket of mesh, well-bucket is slowly immersed in above-mentioned deionized water, after lixiviate twice, mention well-bucket, filter, reclaim filter residue, the filtrate of merging being placed in concentration tank under 0.09MPa pressure, being concentrated into proportion is 1.2, solution to be concentrated is cooled to room temperature and proceeds in stainless steel cask again, 80 degree are added in bucket, pure grain wine 6 kilograms, limit edged stirs, Antrodia camphorata active polysaccharide and triterpene are fully precipitated, leave standstill, precipitation and separation, it is dried in 60 DEG C of air dry ovens and weighs, obtain Antrodia camphorata active polysaccharide and triterpene 32.6 grams, pulverize fine finishining again and obtain Antrodia camphorata active polysaccharide and triterpene fine powder product.And obtain the grain wine liquid material about 7 kilograms of Antrodia camphorata active polysaccharide and triterpene effective ingredient.The output object that corresponding each processing step reclaims will produce the corn feed additive containing Antrodia camphorata active polysaccharide and triterpene effective ingredient respectively, high-purity Antrodia camphorata juice drink and make face nourishing skin care products raw material, and the Antrodia camphorata Wine drink of preparation containing Antrodia camphorata active polysaccharide and triterpene effective ingredient.
Embodiment two
Select the corn decontamination of pollution-free ecological ambient growth, soak to moisture content 60%, for subsequent use with steam sterilizing 20 minutes.
Get the above-mentioned corn prepared 1000 grams, add usual amounts calcium carbonate, potassium dihydrogen phosphate, zinc sulfate, magnesium sulfate and micro-organic germanium and selenium, fully mix evenly, PH is adjusted to be 6.5, being respectively charged into 5 volumes is in 500ml vial, being tamping bottleneck to be placed in pressure cooker under 0.147 MPa, sterilizing 1.5 is little is ground, pressurize 3 hours, treat Pressure Drop to zero, temperature is down to 25 DEG C, take out, in disinfecting action district, by the preprepared wild Antrodia camphorata fungi strain of edible fungi inoculation method access, temperature 25 DEG C is given in sealing, relative humidity 70%, what lucifuge was ventilated cultivates 30 days without in culturing room, the mycelium taken out, this fresh goods mycelium is placed with in the stainless steel well-bucket of mesh, extract in bucket at stainless steel, add 3 kilograms of deionized waters, PH is adjusted to be 7.8, be heated to 100 DEG C, mycelial well-bucket will be housed be placed in one, lixiviate is filtered for 45 minutes, repeat lixiviate more once, filter, reclaim filter residue, by merge filtrate in concentration tank under 0.07MPa pressure, be concentrated into proportion 1.3, solution to be concentrated is cooled to 20 DEG C, proceed in stainless steel cask, add 3 kilograms, 80 DEG C of pure grain wine, limit edged stirs, abundant precipitation, precipitation and separation, dry, obtain Antrodia camphorata active polysaccharide and triterpene 20 grams.Be processed into fine powder product, the filter residue of recovery is processed into feed addictive, the deionized water of the Vacuum Concentration evaporation of recovery, and preparation high-purity Antrodia camphorata and triterpene drink, the grain wine liquid material after precipitation analysed by recovery alcohol prepares various Antrodia camphorata and triterpene wine.
Comprehensive utilization process of the present invention, has possessed large-scale production ability.
Claims (1)
1. the comprehensive utilization process of a high-load Antrodia camphorata fungi activity polysaccharide and triterpene fine powder and byproduct thereof, adopt solid shaped bodies culture medium, through inoculation Antrodia camphorata bacterial classification, prepare Antrodia camphorata mycelium and produce Antrodia camphorata active polysaccharide and triterpene and byproduct byproduct thereof, it is characterized in that comprising following processing step:
One, the full particulate media of solid is prepared
Take corn as raw material, through the permeable immersion of normal temperature, steam removes the pretreated loose corn of a mould material base-material, and add the appropriate conventional nature's mystery salt containing K+, ca++, za++, Mg++, Homogeneous phase mixing makes faintly acid solid shaped bodies culture medium.
Two, mycelium is cultivated
Above-mentioned culture medium is placed in sealed glass container, after autoclaving process, in accessing Antrodia camphorata bacterial classification without mattress environment, sealing, bacteriumization is cultivated in culturing room's environment of uniform temperature, damp condition, go out plentiful Antrodia camphorata mycelium in the superficial growth of tunnel body particulate media, take out mycelium, fresh goods namely with or oven dry to make dry product for subsequent use;
Three, mycelium, lixiviate Antrodia camphorata active polysaccharide and triterpene is dissolved
With 100 of appropriate ratio DEG C of alkalescent deionized waters, dissolve mycelium, each lixiviate 40-50 minute, at least lixiviate filters twice, extracts Antrodia camphorata active polysaccharide and triterpene, and reclaims filter residue;
Four, concentrated, alcohol analyse, output Antrodia camphorata active polysaccharide and triterpene major product, reclaim byproduct
(1) also filtrate, is contained, in 55 DEG C-80 DEG C, under 0.07-0.09MPa pressure, Vacuum Concentration, reclaim the deionized water of concentration process evaporation, and in this concentrate, add 3-4 doubly carry out alcohol to the high-purity grain wine of weight of material and analyse, sedimentation and filtration, precipitation and separation, reclaim the grain liquid material after precipitation and separation, isolated precipitated product is dry, be processed into Antrodia camphorata active polysaccharide and triterpene fine powder, all the other byproducts can make feed addictive (chicken, duck, fish, pig), Antrodia camphorata dew and various Antrodia camphorata Wine drink.
(2), the comprehensive utilization process of a kind of high-load Antrodia camphorata active polysaccharide according to claim 1 and triterpene and byproduct thereof, it is characterized in that the maize raw material adopted comes from without dirty ecological environment, naturally be rich in rare element germanium and selenium, be permeablely dipped to moisture 50-60%.
(3), the comprehensive utilization process of a kind of high-load Antrodia camphorata active polysaccharide according to claim 1 and triterpene fine powder and base substandard goods, its feature Mycelium culture room environmental temperature about 25 DEG C, relative humidity 65-70%.
(4), the comprehensive utilization of a kind of high-load Antrodia camphorata active polysaccharide according to claim 1 and triterpene essence powder and copy product thereof produces, and it is characterized in that lixiviate Antrodia camphorata deionized water and mycelium fresh goods ratio (W/W) are 3-3.5: 1.
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| CN103435713A (en) * | 2013-08-29 | 2013-12-11 | 傅玉 | Comprehensive utilization production process of high-content cordyceps polysaccharide fine powder and by-products thereof |
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| CN106578455A (en) * | 2016-11-29 | 2017-04-26 | 蚌埠达福盛新广饲料有限公司 | Reproductive health feed for adult rams developed by using banana stems and leaves and preparation method thereof |
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| CN106578476A (en) * | 2016-11-29 | 2017-04-26 | 蚌埠达福盛新广饲料有限公司 | Zinc-glutamate chelate and cortex eucommiae extract compounded fattening sheep feed for improving mutton quality and increasing mutton yield and preparation method thereof |
| CN106615759A (en) * | 2016-11-29 | 2017-05-10 | 蚌埠达福盛新广饲料有限公司 | Healthcare goat feed for achieving gastrointestinal mucosal protection and degrading methane poison by diatomite modification and preparation method of healthcare goat feed |
| CN106721204A (en) * | 2016-11-29 | 2017-05-31 | 蚌埠达福盛新广饲料有限公司 | It is a kind of to be used for sterilized anti-diarrhea in goat feeding process and promote health-care feed of stomach and intestine toxin expelling and preparation method thereof |
| CN108014143A (en) * | 2017-11-24 | 2018-05-11 | 浙江工业大学 | A kind of synchronous extraction method of Antrodia camphorata total triterpene and polysaccharide |
| CN108014143B (en) * | 2017-11-24 | 2021-04-06 | 浙江工业大学 | Method for synchronously extracting total triterpenoids and polysaccharides of antrodia camphorata |
| CN109984293A (en) * | 2017-12-29 | 2019-07-09 | 康建生物科技股份有限公司 | Niu Zhangzhi Fermentation ferment brown rice, preparation method and combinations thereof |
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