CN105851943A

CN105851943A - Purely natural blueberry composite powder and preparation method thereof

Info

Publication number: CN105851943A
Application number: CN201610201882.7A
Authority: CN
Inventors: 邵素英
Original assignee: Yiwu Jinyu Information Technology Co Ltd
Current assignee: Yiwu Jinyu Information Technology Co Ltd
Priority date: 2016-04-01
Filing date: 2016-04-01
Publication date: 2016-08-17

Abstract

The invention discloses purely natural blueberry composite powder and a preparation method thereof. The blueberry powder with natural color, flavor and mouth feel is scientifically compounded with functional products including lactobacillus plantarum powder, honey powder, modified dietary fibers, enzyme powder, oligosaccharide and the like, so that the nutrition, the food safety and the health care functions of the blueberry composite powder are effectively improved, and besides, the prebiotic property and the quality stability of the blueberry composite powder are greatly improved. Compared with conventional blueberry composite powder, the blueberry composite powder disclosed by the invention has the advantages that components are simple, the color, the flavor and the mouth feel are natural, the nutrient value is high, the health-care functions are powerful, the content of bioactive substances is high, the viable count of probiotics is high, the prebiotic property is high, the kinds of functional metabolites of probiotics are complete, the content of the functional metabolites of the probiotics is high, the food safety is high, and the quality guarantee period is long.

Description

A kind of pure natural blue berry composite powder and preparation method thereof

Technical field

The present invention relates to blue berry deep processed product, be specifically related to a kind of pure natural blue berry composite powder and preparation method thereof.

Background technology

Blue berry, also known as Pericarpium Citri tangerinae, is commonly called as city fruit, and for perennial fallen leaves or long green fruit tree, tolerance to cold is extremely strong, can resist subzero 50 DEG C Severe cold, originate in North America, Scotland and Russia, be a kind of emerging worldwide small berries fruit tree with high economic worth. Blue berry is the kind that Vaccinium plant Middle nutrition composition is the abundantest, and its fruit is berry, in blueness, and subcircular, sarcocarp is fine and smooth, Sweet acid appropriateness.Fruit, in addition to containing sugar, acid and vitamin C, goes back rich in vitamin E, vitamin A, vitamin B₁、SOD、 The trace such as rare special composition and abundant ferrum, zinc, manganese unit in other fruits such as Arbutin, anthocyanin, protein, fat Element.According to scientific research, Blueberry has the unique effects such as prevent cranial nerve aging, cardiac function enhancing, improving eyesight anticancer.

Blue berry contains a large amount of material to human health, including antimicrobial component, polyphenoils, tannic acid, folic acid and edible fibre Dimension etc..At present, studying more is the flavone compound in blue berry.Research finds, flavone compound has multiple biology Activity and pharmic function, as removed interior free yl, antiinflammatory, reduction cardiovascular disease incidence rate, and anticancer growth Act on its apoptosis of promotion etc..Anthocyanin, flavonol, oligomeric proanthocyanidin, cinnamic acid and stilbene in cranberry and blue berry, Triterpenoid compound (ursolic acid ursolicacid) etc. can suppress sclerosis of blood vessels, blood loss apoplexy, old nerve degeneration etc.. Therefore, blue berry is described as " king of berry ", one of five big healthy fruit that Ye Shi the World Food Programme is recommended.

Along with improving constantly of our people's living standard, more and more higher to food requirement, the secondary industry of blue berry will have extensively Wealthy development prospect, it will become another new industry of 21 century.In recent years, along with people to healthy pay attention to day by day and Blueberry mixed health function understanding is goed deep into by people, and the exploitation of blue berry food cause people and greatly pay close attention to.The most domestic with indigo plant Certain kind of berries fruit is that the food variety of development of raw materials is various, mainly has blue berry milk product, fruit drink, fruit beer, fruit wine, fruit jelly, really Beans, soft sweet, fruitcake and some leisure fooies etc..

Prepare the more of the pure fruit powder of blue berry with blue berry for raw material, utilize vacuum as Chinese patent CN 102273598 A discloses one Lyophilization produces the technique of oral blue berry powder, standby after Blueberry is cleaned, drained；Blueberry after draining-20～ -25 DEG C of pre-freezes, vacuum 10～30mTorr, keep constant temperature 2-20 hour；-20～-25 DEG C, carry out pulverizing and obtain rough Blue berry lyophilized powder.Simplified flowsheet route, the present invention need not long-time high temperature sublimation process and i.e. can get blue berry lyophilized powder, reduces Production cost；Reduce heating step, improve the protection of active substance in blue berry further；The course of processing can avoid hot and humid, The impact of the factor such as illumination, oxidation, the active substance that protection blue berry is whole；Through comparative determination oral indigo plant provided by the present invention Anthocyanidin content contained by the blue berry lyophilized powder that the preparation method of certain kind of berries lyophilized powder is produced is about 3.0mg/g, indigo plant prepared by conventional method Anthocyanidin content contained by certain kind of berries lyophilized powder is about 2.4mg/g, improves anthocyanidin content 15～20%.Although patent disclosed above is adopted Use lyophilizing technique, but do not have good protective agent to protect, will also result in some nutritional labeling freeze denaturations and lose, use simultaneously Grinding mode, high shear force and grinding can cause the loss of heat-sensitive active material.Chinese patent CN 102934765 A discloses Blueberry jam powder, fragrant by white sugar, glucose, blue berry powder, xanthan gum, Konjac glucomannan, citric acid, sodium citrate, blue berry Essence, the red composition of Pericarpium Vitis viniferae, the percentage by weight of each component is as follows: white sugar 40%～50%, glucose 30%～40%, indigo plant Certain kind of berries powder 10%～20%, xanthan gum 1.6%～2.0%, Konjac glucomannan 0.6%～0.8%, citric acid 1.5%～2.0%, sodium citrate 0.8%～1.2%, blueberry flavor 0.5%～0.7%, Pericarpium Vitis viniferae red 0.08%～0.1%, make through dispensing, microwave disinfection.With The blueberry jam powder of the present invention is simple, convenient, fast when making blueberry jam, blueberry jam powder adds quantitative 60 DEG C～65 DEG C pure Water purification, is blueberry jam after dissolving and stirring, and is not required to reheat.The blueberry jam bright color prepared, sour-sweet moderate, There is pure blue berry flavor, without phenomenons such as layering, bleed, crystallizations.Owing to blueberry jam powder is powder product, it is easier to storage and Keeping.Patented foodstuff additive disclosed above is more, there is certain food safety hidden danger.

Also have with blue berry as raw material, add functional raw material and prepare compound health blue berry powder: Chinese patent CN103798665 A Disclose a kind of health care blue berry powder and preparation method thereof, it is characterised in that be prepared by the raw materials in: blue berry 100-110, Thallus Laminariae (Thallus Eckloniae) 1-1.2, flower of Radix Notoginseng 2-3, Herba Cirsii 2.5-4, Rhizoma Chuanxiong 1.8-2, Radix Paeoniae Alba 2-2.2, Radix Glycyrrhizae 4-5, Radix rubi coreani 2-2.4, DIGEN 2.8-3, Fructus Cucurbitae moschatae 10-12, Radix Ipomoeae 8-9, milk 3-4, Pleurotus ostreatus 20-22, original juice of red bayberry 20-22, rice 10-11, Lactic acid bacteria 0.2-0.3, walnut powder 10-11, nourishing additive agent 8-9.Preparation technology of the present invention is simple, the blue berry powder produced Delicate mouthfeel, and while the nutrition maintaining blue berry, the nutrition of other adjuvants such as Radix Ipomoeae, rice is incorporated wherein so that Nutriture value of the present invention is high, additionally, the present invention with the addition of multiple Chinese herbal medicine in the course of processing, has health-care effect, often eats Can reach effect of suppressing the hyperactive liver blood pressure lowering, promoting flow of QI and blood, heat-clearing and toxic substances removing.Chinese patent CN 103815396 A discloses a kind of health care indigo plant Certain kind of berries powder and preparation method thereof, it is characterised in that be prepared by the raw materials in: blue berry 100-110, Herba Taraxaci 2-3, south Melon root 1-2, Flos puerariae lobatae 4-5, Rhizoma Anemarrhenae 2-3, Folium Lycii 2-3, Dioscorea subcalva Prain et Burhll 1.5-2, Folium Aeschynomense indicae 2-3, Herba Coriandri 10-11, Pumpkin 8-9, broad bean paste 3-4, Radix Polygalae Crotalarioidis 12-13, silkie soup 50-52, Oryza glutinosa 40-45, Sorghum vulgare Pers. flour 10-11, big Oleum Glycines 3-4, nourishing additive agent 4-5.Delicate mouthfeel of the present invention, unique flavor, nutritious, the wherein pig of present invention interpolation Large intestine can moisturize qi-restoratives, silkie soup can nourishing the liver to improve visual acuity, blood-enriching face-nourishing, additionally, the present invention also added multiple Chinese herbal medicine, wherein Herba Taraxaci can heat-clearing and toxic substances removing, Folium Lycii can qi-restoratives benefit essence, Dioscorea subcalva Prain et Burhll can spleen invigorating dry, invigorating the lung and the kidney, often the edible present invention up to Effect to strong health.It is more that patent disclosed above adds the compositions such as Chinese medicine, complicated components, although have certain function Property, but have impact on natural mouthfeel and the color and luster of blue berry, inapplicable most crowd's consumption.

Also have and prepare other type powder with blue berry for raw material, such as: Chinese patent CN 105248724 A discloses a kind of blue berry Milk tea powder, calculates in parts by weight, including following raw material: non-dairy creamer 40-50 part, green tea powder 10-20 part, crystal sugar 10-20 Part, blue berry powder 10-20 part, tomato meal 5-10 part, Punica granatum L. 5-10 part, sodium carboxymethyl cellulose 0.1-0.3 part, sugarcane Sugar fatty acid ester 0.1-0.3 part, milk of the present invention is strong, nutritious, has the effect of the improving eyesight that clears away heart-fire.Disclosed above Patented foodstuff additive is more, and blue berry content is few, however it remains food safety hidden danger and mouthfeel suitability limit.

To sum up, with blue berry as primary raw material, a kind of mouthfeel of preparation and color and luster are natural, and nutritional labeling and bioactive substance content are high, Component strong pure natural blue berry composite powder simple, functional is still a kind of necessary.

Summary of the invention

Solved by the invention technical problem is that the defect overcoming existing blue berry powder and preparation thereof, prepare one with blue berry for primary raw material Planting mouthfeel and color and luster is natural, nutritional labeling and bioactive substance content are high, and component strong pure natural blue berry simple, functional is multiple Close powder.

In order to achieve the above object, the present invention is by the following technical solutions:

A kind of pure natural blue berry composite powder, is mainly prepared by the raw material of following parts by weight:

Blue berry powder 50-70 part, Mel powder 20-30 part, modified dietary fiber 8-15 part, oligosaccharide 6-12 part, plant breast bar Mycopowder 14-20 part, ferment powder 5-10 part, maltodextrin 5-10 part；

Preferably, described pure natural blue berry composite powder, mainly prepared by the raw material of following parts by weight:

Blue berry powder 55-65 part, Mel powder 23-27 part, modified dietary fiber 11-13 part, oligosaccharide 8-10 part, plant breast bar Mycopowder 16-18 part, ferment powder 7-9 part, maltodextrin 6-8 part；

It is highly preferred that described pure natural blue berry composite powder, mainly prepared by the raw material of following parts by weight:

Blue berry powder 60 parts, Mel powder 25 parts, modified dietary fiber 12 parts, oligosaccharide 9 parts, 17 parts of Lactobacillus plantarum powder, Ferment powder 8 parts, maltodextrin 7 parts；

Further, described blue berry powder be through the instantaneous enzyme denaturing of microwave high-temperature, fixation and ultrasonic waves for cleaning, sericin peptide taken cryoprotection, The extract at low temperature technology such as high-pressure pulse electric extraction, ultrasonic assistant microwave extraction, biological enzymolysis and prepare, protect to greatest extent The natural colored of blue berry, mouthfeel and bioactive ingredients thereof are stayed；

Preferably, the preparation method of described blue berry powder, comprise the following steps, blue berry is put into that to fill 0.1-0.3% sodium bicarbonate molten In the ultrasonic washing unit of liquid, 200-400W, 35-45KHz clean 5-10min at room temperature, drain, and each blue berry is any Being cut into four parts, single flat is layered in microwave dryer in power 3-5kW, temperature 120-130 DEG C, is dried 3-5s, then soaks Bubble 8-10min in the sericin peptide taken solution that mass percent concentration is 8-12%, takes out, after-18--22 DEG C of freezing 30-50min Pulverize immediately, ground product particle diameter 0.3-0.5mm, be subsequently added into the water of ground product quality 1-3 times, with breast acid for adjusting pH value For 3.5-5.5, at room temperature in electric field intensity 25-35kV/cm, burst length 300-500 μ s, pulse frequency 200-300Hz bar High voltage pulse electric field processing 20-30min is carried out under part；Then under the conditions of power 150-300W, carry out microwave exposure in room temperature to carry Take 15-20min, simultaneously at power 200-300W, under the conditions of frequency 30-40KHz, carry out ultrasonic assistant extraction；Add and extract The mixed enzyme of liquid quality 1.5-2.5%, in 40-50 DEG C of enzymolysis 30-50min, enzymolysis solution filters, and filtrate reduced in volume, freezing are done Dry is that 5-8% i.e. obtains blue berry powder to moisture；

Described mixed enzyme is that cellulase, protease, pectase, tannase 2-4:1-3:1-3:1-2 in mass ratio uniformly mixes.

Further, described Mel powder is with Mel as primary raw material, and science compounds dryingaid, through ultrasonic dissolution, colloid mill, height Pressing homogenizing and drying process with atomizing to prepare, the Mel powder prepared is notable with the Mel nutritional labeling difference in change opposite sex, recovery rate Up to more than 95%, efficiency is high；

Preferably, the preparation method of described Mel powder, comprise the steps: Mel, maltodextrin, beta-schardinger dextrin-by quality More uniform mixing than 5-7:2-4:0.5-1.5, add the deionized water of 50 DEG C, fully dissolve so that the solid content of mixed liquor For 32-35%, mixed liquor carries out ultrasonic Treatment 5-10min under power 200-400W, frequency 30-40KHz, room temperature condition, Then through colloid mill circular grinding 2-4 time, homogenizing, homogenization pressure 25-30MPa, homogenizing temperature 50-60 DEG C, homogenizing fluid is through spray Mist drying machine is in inlet temperature 180-200 DEG C, and leaving air temp 70-90 DEG C is drying to obtain Mel powder, and Mel powder particle diameter is 20-40 mesh.

Further, described modified dietary fiber is the tool that dietary fiber is processed through physics, chemical or biological method and obtained The soluble fiber cellulose content having strong retentiveness, dilatancy, thickening property, adsorptivity and network to enrich is high, biological activity is strong, Have important, the cellulose of positive role to human body beneficial flora, compared with full diet fiber, its biological action is higher Greatly, the shelf-life of blue berry powder can be also greatly prolonged；

Preferably, described modified fibre is through life by one or more in inulin, apple fiber, Herba avenae fatuae fiber, Semen Tritici aestivi fiber Thing enzyme enzymolysis and obtain；

It is highly preferred that the preparation method of described modified dietary fiber, comprise the following steps: by inulin, Semen Tritici aestivi fiber, Herba bromi japonici fibre Tie up 5-7:2-4:1-3 in mass ratio uniformly to mix, add the water of its quality 3-7 times, room temperature 100-300W, 35-40KHz condition Supersound extraction 10-15min, then in electric field intensity 20-40kV/cm, burst length 300-500 μ s, pulse frequency 200-400Hz Under the conditions of carry out high voltage pulse electric field processing 10-15min；It is 4.5-6.5 with breast acid for adjusting pH value, adds mixture quality The enzyme of 0.1-0.3%, in 45-55 DEG C of enzymolysis 20-48min；Enzymolysis solution filters, and filtrate reduced in volume, lyophilization are to water Dividing content is that 5-8% i.e. obtains modified dietary fiber；

Described enzyme is that cellulase, xylanase, laccase, pectase 1-3:1-3:1-2:1-2 in mass ratio uniformly mixes.

Further, described oligosaccharide is that oligofructose, stachyose, Raffinose, oligomeric xylose, oligomeric galactose, Semen sojae atricolor are low In polysaccharide, oligomeric isomaltose, Oligomeric maltose at least one；

Preferably, described oligosaccharide parts by weight consist of oligofructose 40-50 part, Oligomeric maltose 30-40 part, Raffinose 20-40 part, soybean oligo saccharide 16-18 part, oligomeric galactose 10-15 part, oligomeric xylose 10-15 part, oligomeric isomaltose 10-15 part, stachyose 8-12 part.

Further, described Lactobacillus plantarum powder is with Lactobacillus plantarum CGMCC NO.11763 for starting strain according to a conventional method Preparation, its viable bacteria content is: 7 × 10¹²-9×10¹²cfu/g；The cryoprotective agent that wherein freeze drying process uses is to contain The animal or plant of antifreeze protein is raw material, through high-pressure pulse electric extraction, ultrasonic assistant microwave extraction and compound enzyme enzymolysis Prepare, Lactobacillus plantarum powder can be effectively improved at freezing dry process Viable detection；

Preferably, described protectant preparation method, comprise the steps: winter rye, Caulis et Folium Ammopiptanthi Mongolici, sharkskin collagen protein Being respectively washed, drain, 8-10:3-5:2-4 in mass ratio uniformly mixes, and the pH value adding mixed material quality 0.1-1 times is The lactic acid moistening 3-8h of 3.8-4.5, pulverizes after-18--22 DEG C of freezing 1-2h immediately, freezing thickness of feed layer 3-5cm, powder Mince particle diameter 0.5-3mm, is subsequently added into the water of ground product quality 10-20 times, is 3.5-5.5 with breast acid for adjusting pH value, in room temperature Under at electric field intensity 25-35kV/cm, burst length 300-500 μ s, carry out high-voltage pulse electric under the conditions of pulse frequency 200-300Hz Field processes 20-30min；Then under the conditions of power 150-300W, carry out microwave irradiation and extraction 15-20min in room temperature, exist simultaneously Power 200-300W, carries out ultrasonic assistant extraction under the conditions of frequency 30-40KHz；Add the compound enzyme of extracting solution quality 1-2%, In 45-55 DEG C of enzymolysis 30-50min；Enzymolysis solution filters, filtrate concentrates, low-temperature grinding to particle diameter is that 0.1-0.3mm i.e. obtains protection Agent；

Described compound enzyme is cellulase, protease, amylase, pectase, tannase 2-4:1-3:1-3:1-2:1-2 in mass ratio Uniformly mixing；

Extract it is highly preferred that described microwave irradiation and extraction is batch (-type), i.e. microwave exposure 10s, be spaced 20s.

Further, described ferment powder preparation method is as follows: the fermentation liquid using pickle fermentation to produce discharge in latter stage is raw material, mistake Filtering the big grain materials such as the dish leaf in fermentation liquid and obtain fermented liquid, fermented liquid uses the drying means preparations such as lyophilization to obtain Obtain ferment powder.Cryodesiccated condition is :-30--40 degree pre-freeze 6 hours；Then lyophilizing is until water content is less than 5%.Pickles Production method is prepared with reference to Chinese patent 201110421967.3 or 201210310308.7.

Another object of the present invention is to provide the preparation method of above-mentioned pure natural blue berry composite powder, comprises the steps: according to recipe ratio Example, weighs each raw material, ferment powder, Lactobacillus plantarum powder, blue berry powder, maltodextrin, Mel powder, oligosaccharide is added successively V-type blending tank uniformly mixes 25-35min, speed of agitator 20-40r/min, is subsequently adding modified dietary fiber and uniformly mixes 12-18min, Speed of agitator 40-60r/min, sterile filling, seals, packs and i.e. obtain pure natural blue berry composite powder；

Preferably, described sterile filling uses automatic powdery filling machine fill, uses simultaneously⁶⁰Co irradiation sterilization, irradiation dose 4kGy；

Preferably, described pure natural blue berry composite powder uses Aluminum-plastic composite bag fill；Ultraviolet is used before described Aluminum-plastic composite bag fill Line or ozonization 20-40min.

The pure natural blue berry composite powder Lactobacillus plantarum viable bacteria content prepared through said method is 6.84×10¹¹-9.49×10¹¹cfu/g。

It is micro-that Lactobacillus plantarum of the present invention (Lactobacillus plantarum) XH is preserved in China on November 30th, 2015 CGMCC (is called for short) in biological inoculum preservation administration committee's common micro-organisms center, and preserving number is CGMCC NO.11763, preservation ground Location is: North Star West Road 1, Chaoyang District, city of BeiJing, China institute 3, Institute of Microorganism, Academia Sinica, postcode: 100101.

Lactobacillus plantarum probiotic properties is as follows:

Lactobacillus plantarum CGMCC NO.11763 provided by the present invention is found under conditions of pH is 1.50 survive through experiment, Still in existing state after 1% cholate is cultivated 4 hours；Lactobacillus plantarum CGMCC NO.11763 degrading nitrite speed is fast, Capacity of decomposition reaches 10.9mg/h/kg, and this strain is when producing Pickles, and whole sweat nitrite concentration is at 4.8mg/kg Below；CGMCC NO.11763, after fermentation 60h hour, can reach 64.76% to degrading rate of cholesterol.CGMCC NO.11763 Adhering capacity measure from coagulation rate be 95.71%.

Lactobacillus plantarum CGMCC NO.11763 is to cholesterol degradation capability study and mensuration:

Take 1ml CGMCC NO.11763 mother solution and be inoculated in the MRS cholesterol fluid medium (cholesterol level of 10mL 0.1mg/ml, pH 6.2) in, it is standby, to access 1mL sterilized water that the constant temperature standing of 37 DEG C cultivates 20h, 40h, 60h respectively MRS cholesterol culture medium is comparison, takes bacteria liquid sample and the comparison each 1ml of liquid, 9000r/min, 4 DEG C of above cultivation different time Under be centrifuged 10min, obtain fermented supernatant fluid, o-phthalaldehyde method measures in supernatant cholesterol level (particularly as follows: take on each Clear liquid 0.1ml, in corresponding test tube, adds glacial acetic acid 0.3ml, and o-phthalaldehyde(OPA) 0.15ml of 1mg/ml is slowly added into dense sulfur Acid 1.0ml, mix homogeneously.Room temperature stands 10min, surveys light absorption value under 550nm).Each process 3 repetition, with equally Method makes cholesterol standard curve, calculates cholesterol level and degradation rate in supernatant, the results are shown in Table 1.Understand, CGMCCNO.11763 has good Degradation to cholesterol, and after fermentation 60h hour, degradation rate can reach 64.76%.

The table 1 degraded situation to cholesterol.

Degradation time (h)	0	20h	40h	60h
					Cholesterol level (mg/ml)	0.2273±0.0058	0.1356±0.0018	0.1011±0.0094	0.801±0.0231
Degrading rate of cholesterol %		40.34%	55.52%	64.76%

The bile tolerance test of Lactobacillus plantarum CGMCC NO.11763 bacterial strain:

Take CGMCCNO.11763 bacterium solution 1mL inoculation strain in containing different cholate (concentration gradients is 0.0%, 0.2%, 0.4%, 0.6%, 0.8%, 1%) 10mL MRS fluid medium (PH=6.4), be placed at 37 DEG C and cultivate 0 respectively, 2,4h, often Individual process 3 repetition.Respectively take 1ml sample bacterium solution to mix in 9ml normal saline, prepare dilution factor solution, take 0.1ml dilute Release liquid to be coated with in MRS, be inverted in 37 DEG C of biochemical cultivation cases and cultivate 48 hours (each dilution factor do 3 parallel) records Calculate the several number of bacterium on flat board.The results are shown in Table 2.The increment of bacterium is still after gallbladder salinity is 1% process 4h to understand this bacterium

Reach 0.59 ± 0.92 × 10⁷(cfu/ml), there is good bile tolerance ability.

Table 2 bile tolerance ability detection [(± s) × 10⁷cfu/ml]

The acid resistance test of Lactobacillus plantarum CGMCC NO.11763 bacterial strain

Take HLX37 mother solution by 1ml inoculation strain in different pH value (pH gradient is 1.5,2.0,2.5,3.0,3.5,4.0) 10mLMRS fluid medium, be placed at 37 DEG C and cultivate 0 respectively, 2,4h, each process 3 repetition.Respectively take 1ml sample Product bacterium solution mixes in 9ml normal saline, prepares dilute solution, takes 0.1ml diluent and is coated with in MRS, in 37 DEG C Biochemical cultivation case is inverted the bacterium colony number cultivated on 48 hours (each dilution factor do 3 parallel) record flat board.Result is shown in Table 3.Illustrate that this bacterium has the strongest acid-fast ability.

Table 3 acid-fast ability detection [(± s) × 10⁷cfu/ml]

The Adhering capacity of Lactobacillus plantarum CGMCC NO.11763 measures

Cultivate CGMCC NO.11763 (MRS fluid medium), bacillus coli DH 5 alpha (LB fluid medium) 24h must send out Ferment liquid, is respectively placed in 3000r/min, centrifugal 10min at 4 DEG C, collects bacterium mud, delay with the sterile phosphate of pH=7.0 respectively Rush liquid (PBS) washing bacterium mud 2 times (in bacterium colony, i.e. add PBS, after concussion mix homogeneously, be placed in 3000r/min, 4 DEG C Under be centrifuged 10min, collect thalline).From coagulation rate (%): with aseptic PBS, bacterium mud CGMCC NO.11763 is formed in Light absorption value at wavelength 600nm is suspension bacteria liquid and the bacteria suspension of 0.4 ± 0.1 (A 0), measures extinction after standing 24h Value A 24, is (A 0 A 24)/A 0 from coagulation rate (%) formula.；His coagulation rate (%): by CGMCC NO.11763 It is adjusted to, with the outstanding bacterium solution of bacillus coli DH 5 alpha, the mixing that the light absorption value at wavelength 600nm is 0.6 ± 0.1 (A 0) hang Floating bacterium solution.Measuring light absorption value A 24 after standing 24H, his coagulation rate (%) formula is (A 0 A 24)/A 0.Survey Surely the results are shown in Table 5, it is known that CGMCC NO.11763 is 95.71% from coagulation rate, has the strongest Adhering capacity.

Table 4 Adhering capacity table

The bacterial strain physiological property of Lactobacillus plantarum CGMCC NO.11763

Described Lactobacillus plantarum (Lactobacillus plantarum) XH is preserved in Chinese micro-life on November 30th, 2015 CGMCC (is called for short) in thing culture presevation administration committee's common micro-organisms center, and preserving number is CGMCC NO.11763, preservation address For: North Star West Road 1, Chaoyang District, city of BeiJing, China institute 3, Institute of Microorganism, Academia Sinica, postcode: 100101.

This bacterial strain feature is as follows: examine under a microscope, and this bacterial strain is rod-short, and Gram’s staining is positive, atrichia, no Produce spore；On solid medium, this bacterium bacterium colony is white, and smooth surface is fine and close, and form is circular, and edge is more neat.

Physicochemical characteristics is: catalase (-), gelatin liquefaction (-), indole experiment (+), mobility (-), fermentation gas (-), nitrate reductase (-), fermentation gas (-), product hydrogen sulfide gas (-), pH4.0MRS culture medium grows (+). It is accredited as Lactobacillus plantarum (Lactobacillus plantarum), named Lactobacillus plantarum through Physiology and biochemistry (Lactobacillus plantarum)XH。

Bacterial strain can at 57 DEG C well-grown, glucose tolerance is 275g/L.

Lactobacillus plantarum of the present invention is by gathering people Li Jianshu, and isolated in Yoghourt from Xinjiang Uygur fellow-villager family, during collection Between on June 2nd, 2015.

Lactobacillus plantarum CGMCC NO.11763 of the present invention is found under conditions of pH is 1.50 survive, 1% through experiment Cholate is cultivated after 4 hours still in existing state；Lactobacillus plantarum CGMCC NO.11763 degrading nitrite speed is fast, decomposes Ability reaches 10.9mg/h/kg, this strain produce Pickles time, whole sweat nitrite concentration 4.8mg/kg with Under；CGMCCNO.11763, after fermentation 60h hour, can reach 64.76% to degrading rate of cholesterol.CGMCC NO.11763 sticks Attached ability measure from coagulation rate be 95.71%, bacterial strain can at 57 DEG C well-grown, glucose tolerance is 275g/L.

Beneficial effect:

Pure natural blue berry composite powder of the present invention will have blue berry powder and Lactobacillus plantarum powder, the Mel of natural colored, local flavor and mouthfeel The functional product science such as powder, modified dietary fiber, ferment powder, oligosaccharide compound, and are not only effectively increased blue berry composite powder Trophism, foodsafety and health care, and significantly improve the probiotic of blue berry composite powder and quality stability, with existing Having blue berry composite powder to compare: its component is simple, and color and luster, local flavor and mouthfeel are natural, are of high nutritive value, health care is strong, biological Activity substance content is high, and probiotics viable bacteria quantity is high, probiotic by force, and probiotic bacteria functional metabolic species is complete, content is high, food Product safety is high, long shelf-life.Concrete test effect is shown in embodiment seven-ten two, and concrete component effect is as follows:

1. the Lactobacillus plantarum CGMCC NO.11763 of the present invention is found under conditions of pH is 1.50 survive through experiment, Still in existing state after 1% cholate cultivation 4 hours；Lactobacillus plantarum CGMCC NO.11763 degrading nitrite speed is fast, point Solution ability reaches 10.9mg/h/kg, and this strain is when producing Pickles, and whole sweat nitrite concentration is at 4.8mg/kg Below；CGMCC NO.11763, after fermentation 60h hour, can reach 64.76% to degrading rate of cholesterol.CGMCC NO.11763 Adhering capacity measure from coagulation rate be 95.71%.

2. the cryoprotective agent that the present invention uses when preparing Lactobacillus plantarum powder is by the winter rye higher containing antifreeze protein, sand Ilicis Purpureae and sharkskin collagen protein science compound, through high-pressure pulse electric extraction, ultrasonic assistant microwave extraction and biological enzymolysis Preparing, omnidistance extract at low temperature, antifreeze protein extraction ratio is high, and loss is few, the protective agent antifreeze peptide content obtained is high, kind is complete, Functional strong, cryoprotective effects is good, improves the viable bacteria content in Lactobacillus plantarum powder.

3. the blue berry powder that prepared by the present invention uses ultrasonic cleaning that blue berry carries out parasite killing to go out ovum, killing microorganisms, removal pesticide residues With heavy metal ion etc., substantially increase the foodsafety of blue berry powder；Use microwave high-temperature instantaneous dry, cut making blue berry It is mainly enzyme denaturing, fixation while sheet explosion puffing drying, remains the natural colored of blue berry to greatest extent, after microwave color fixing Blue berry natural colored is highly stable in the follow-up course of processing, will not variable color, fade, color and luster is the most bright-coloured；Containing sericin The aqueous solution of peptide soaks rehydration, the loss of blueberry activated material that is chilled and that cause can be reduced to greatest extent, improve indigo plant The extraction ratio of certain kind of berries effective ingredient, also lays a good foundation at storage freezing-resistance for blue berry composite powder simultaneously；By high-voltage pulse electric The extract at low temperature technology such as field, microwave, ultrasonic, biological enzymolysis organically combine, and can retain the biological active matter of blue berry to greatest extent Matter and flavor component.The blue berry powder anthocyanidin content of preparation is at more than 3.5mmg/g.

4. the modified dietary fiber that prepared by the present invention is that dietary fiber is processed through physics, chemical or biological method and obtained, Soluble fiber cellulose content is high, biological activity strong, have important, the cellulose of positive role to beneficial bacteria of intestinal tract group, and commonly Dietary fiber is compared, and its biological action is more powerful: 1) soluble fiber cellulose content is high, it is easier to utilized by probiotic bacteria, can Improve probiotic bacteria in the growth of intestinal and fertility, increase kind and the quantity of probiotic bacteria flora, reduce large intestine pH value, improve Intestine microenvironment；2) powerful absorbability, after modified, the specific surface area of cellulose increases, and network is enriched, Absorption affinity strengthens, and the organic molecule ability chelating, adsorbing cholesterol and bile acids is higher, the suppression human body absorption to them；3) Ion-exchange capacity strengthens, and to metallic element, particularly heavy metal element adsorption effect is higher；4) retentiveness, dilatancy, increasing Consistence is higher and acid and alkali, alkali, the impact of salt, 5) regulate and maintain the resident time of intestinal microbial population, strengthen intestinal digestion and Absorbability, improves body immunity, finally significantly improves digestion and the absorbance of blue berry composite powder effective ingredient；6) stomach is promoted Enterokinesia, slows down and eliminates the untoward reaction such as flatulence, abdominal distention；7) embedding effect is strong, can effectively prevent external environment (oxygen, Temperature, illumination, humidity etc.) factor impact on product quality, stabilize the biological activity of product, extend guaranteeing the quality of product Phase.

5. the Mel powder that prepared by the present invention is with Mel as primary raw material, and science compounds dryingaid, through ultrasonic dissolution, can make Mel Uniformly mix with dryingaid, merge fully, under conditions of high sugar content, solid content are higher, prepare stable, homogeneous honeybee Honey solution, the Mel powder hygroscopicity prepared after colloid mill, high pressure homogenize and spray drying is little, it is simple to and the mixing of other component raw material, Long shelf-life, nutritional labeling is almost without loss, and recovery rate is up to more than 95%, and efficiency is high.

6. the ferment powder that prepared by the present invention does not contain only the purebred benefits such as acetobacter, bacillus bifidus, yeast and lactobacillus rhamnosus Raw bacterium, and containing more, more useful participate in during pickle fermentation and the multiple wild microbial strains that produces, it is raw Thing is active and probiotic more comprehensively with powerful, and the Lactobacillus plantarum powder science prepared with the present invention compounds, and its effect is more significantly.

The preparation method technique of blue berry composite powder the most of the present invention is simple, easy to operate, low for equipment requirements, can industrialization and scale Produce, modified dietary fiber is finally mixed, has played its powerful embedding effect so that the character of product is more stable, guarantee the quality Phase is longer.

It should be noted that pure natural blue berry composite powder of the present invention has the technical effect that each component technical characteristic and method feature are mutually assisted Result same, that interact, the superposition of not simple technical characteristic (component function), organic knot of each component technical characteristic Close and the collaborative effect produced is considerably beyond each monotechnics feature functionality and the superposition of effect, have the most advanced and practical Property.

Detailed description of the invention

The present invention is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention is this Method well known to skilled person.It addition, embodiment is interpreted as illustrative, and unrestricted the scope of the present invention, The spirit and scope of the invention are limited only by the claims that follow.To those skilled in the art, real without departing substantially from the present invention On the premise of matter and scope, the various changes or the change that carry out the material component in these embodiments and consumption fall within this Bright protection domain.

Embodiment one:

Prepared by raw material:

1, the preparation of Mel powder

Its preparation method comprises the steps:

Mel, maltodextrin, beta-schardinger dextrin-6:3:1 in mass ratio is uniformly mixed, adds the deionized water of 50 DEG C, the most molten Solve so that the solid content of mixed liquor is 33%, and mixed liquor surpasses under power 300W, frequency 35KHz, room temperature condition Sonicated 8min, then through colloid mill circular grinding 3 times, homogenizing, homogenization pressure 28MPa, homogenizing temperature 55 DEG C, homogenizing The spray-dried machine of liquid is drying to obtain Mel powder in inlet temperature 190 DEG C, leaving air temp 80 DEG C, and Mel powder particle diameter is 30 mesh.

2, the preparation of modified dietary fiber

Its preparation method comprises the following steps:

Inulin, Semen Tritici aestivi fiber, Herba avenae fatuae fiber 6:3:2 in mass ratio is uniformly mixed, adds the water of its quality 5 times, room temperature 200W, 38KHz condition supersound extraction 13min, then in electric field intensity 30kV/cm, burst length 400 μ s, pulse frequency 300Hz condition Under carry out high voltage pulse electric field processing 13min；It is 5.5 with breast acid for adjusting pH value, adds the enzyme of mixture quality 0.2%, In 50 DEG C of enzymolysis 34min；Enzymolysis solution filters, and filtrate reduced in volume, lyophilization to moisture are 6.5% i.e. to obtain modified meals Fiber；

Above-mentioned enzyme is that cellulase, xylanase, laccase, pectase 2:2:1.5:1.5 in mass ratio uniformly mixes.

3, the preparation of Lactobacillus plantarum powder

Lactobacillus plantarum powder is prepared with Lactobacillus plantarum CGMCC NO.11763 according to a conventional method for starting strain, and its viable bacteria contains Amount is: 8 × 10¹²cfu/g；The cryoprotective agent that wherein freeze drying process uses is former with the animal or plant containing antifreeze protein Material, prepared through high-pressure pulse electric extraction, ultrasonic assistant microwave extraction and compound enzyme enzymolysis, plant breast can be effectively improved Bacillus powder is at freezing dry process Viable detection；

Above-mentioned protectant preparation method, comprises the steps:

Winter rye, Caulis et Folium Ammopiptanthi Mongolici, sharkskin collagen protein are respectively washed, are drained, and 9:4:3 in mass ratio uniformly mixes, and adds The pH value that mixed material quality is 0.5 times is the lactic acid moistening 5h of 4.2, pulverizes immediately after-20 DEG C of freezing 1.5h, freezing Thickness of feed layer 4cm, ground product particle diameter 2mm, it is subsequently added into the water of ground product quality 15 times, is 4.5 with breast acid for adjusting pH value, At room temperature in electric field intensity 30kV/cm, burst length 400 μ s, carry out at high-pressure pulse electric under the conditions of pulse frequency 250Hz Reason 25min；Then under the conditions of power 225W, microwave irradiation and extraction 18min, its microwave exposure 10s are carried out in room temperature, interval 20s, simultaneously at power 250W, carries out ultrasonic assistant extraction under the conditions of frequency 35KHz；Add answering of extracting solution quality 1.5% Join enzyme, in 50 DEG C of enzymolysis 40min；Enzymolysis solution filters, filtrate concentrates, low-temperature grinding to particle diameter is that 0.2mm i.e. obtains protective agent；

Above-mentioned compound enzyme is that cellulase, protease, amylase, pectase, tannase 3:2:2:1.5:1.5 in mass ratio is equal Even mixing.

4, the preparation of ferment powder

Its preparation method comprises the following steps:

The big grain materials such as the fermentation liquid using pickle fermentation to produce discharge in latter stage is raw material, the dish leaf being filtered to remove in fermentation liquid obtain Fermented liquid, fermented liquid uses the drying meanss such as lyophilization to prepare ferment powder.Cryodesiccated condition is :-35 degree Pre-freeze 6 hours；Then lyophilizing is until water content 3%.Pickle production method is prepared with reference to Chinese patent 201110421967.3.

Mel powder that following example two to six are used, modified dietary fiber, Lactobacillus plantarum powder, ferment powder are by embodiment One preparation.

Embodiment two:

Wherein oligosaccharide parts by weight consist of oligofructose 45 parts, Oligomeric maltose 35 parts, Raffinose 30 parts, soy oligosaccharides Sugar 17 parts, oligomeric galactose 13 parts, oligomeric xylose 13 parts, oligomeric isomaltose 13 parts, 10 parts of stachyose；

Wherein the preparation method of blue berry powder, comprises the following steps,

Being put into by blue berry in the ultrasonic washing unit filling 0.2% sodium bicarbonate solution, 300W, 40KHz clean 8min at room temperature, Draining, each blue berry is arbitrarily cut into four parts, and single flat is layered in microwave dryer in power 4kW, temperature 125 DEG C, is dried 4s, is then immersed in 9min in the sericin peptide taken solution that mass percent concentration is 10%, takes out, vertical after-20 DEG C of freezing 40min I.e. pulverize, ground product particle diameter 0.4mm, be subsequently added into the water of ground product quality 2 times, be 4.5 with breast acid for adjusting pH value, in In electric field intensity 30kV/cm under room temperature, burst length 400 μ s, carry out high voltage pulse electric field processing under the conditions of pulse frequency 250Hz 25min；Then under the conditions of power 200W, microwave irradiation and extraction 18min is carried out in room temperature, simultaneously in power 250W, frequency 35KHz Under the conditions of carry out ultrasonic assistant extraction；Add the mixed enzyme of extracting solution quality 2.0%, in 45 DEG C of enzymolysis 40min, enzymolysis solution mistake Filter, filtrate reduced in volume, lyophilization to moisture are 7% i.e. to obtain blue berry powder；

Above-mentioned mixed enzyme is that cellulase, protease, pectase, tannase 3:2:2:1.5 in mass ratio uniformly mixes.

The preparation method of pure natural blue berry composite powder, comprises the steps:

According to formula proportion, weigh each raw material, successively by ferment powder, Lactobacillus plantarum powder, blue berry powder, maltodextrin, Mel Powder, oligosaccharide add V-type blending tank and uniformly mix 30min, speed of agitator 30r/min, are subsequently adding modified dietary fiber and uniformly mix 15min, speed of agitator 50r/min, sterile filling, seal, pack and i.e. obtain pure natural blue berry composite powder；

Above-mentioned sterile filling uses automatic powdery filling machine fill, uses simultaneously⁶⁰Co irradiation sterilization, irradiation dose 4kGy；

Above-mentioned pure natural blue berry composite powder uses Aluminum-plastic composite bag fill；Disinfection by ultraviolet light is used before described Aluminum-plastic composite bag fill 30min。

The pure natural blue berry composite powder Lactobacillus plantarum viable bacteria content prepared through said method is 9.49 × 10¹¹cfu/g。

Embodiment three:

Blue berry powder 55 parts, Mel powder 23 parts, modified dietary fiber 11 parts, oligosaccharide 8 parts, 16 parts of Lactobacillus plantarum powder, Ferment powder 7 parts, maltodextrin 6 parts；

Wherein oligosaccharide parts by weight consist of oligofructose 40 parts, Oligomeric maltose 30 parts, Raffinose 20 parts, soy oligosaccharides Sugar 16 parts, oligomeric galactose 10 parts, oligomeric xylose 10 parts, oligomeric isomaltose 10 parts, 8 parts of stachyose；

The wherein preparation method of blue berry powder, comprises the following steps:

Being put into by blue berry in the ultrasonic washing unit filling 0.1% sodium bicarbonate solution, 200W, 35KHz clean 5min at room temperature, Draining, each blue berry is arbitrarily cut into four parts, and single flat is layered in microwave dryer in power 3kW, temperature 120 DEG C, is dried 3s, is then immersed in 8min in the sericin peptide taken solution that mass percent concentration is 8%, takes out, after-18 DEG C of freezing 30min immediately Pulverize, ground product particle diameter 0.3mm, be subsequently added into the water of ground product quality 1 times, be 3.5 with breast acid for adjusting pH value, in room In electric field intensity 25kV/cm under temperature, burst length 300 μ s, carry out high voltage pulse electric field processing under the conditions of pulse frequency 200Hz 20min；Then under the conditions of power 150W, microwave irradiation and extraction 15min is carried out in room temperature, simultaneously in power 200W, frequency 30KHz Under the conditions of carry out ultrasonic assistant extraction；Add the mixed enzyme of extracting solution quality 1.5%, in 40 DEG C of enzymolysis 30min, enzymolysis solution mistake Filter, filtrate reduced in volume, lyophilization to moisture are 5% i.e. to obtain blue berry powder；

Above-mentioned mixed enzyme is that cellulase, protease, pectase, tannase 2:1:1:1 in mass ratio uniformly mixes.

According to formula proportion, weigh each raw material, successively by ferment powder, Lactobacillus plantarum powder, blue berry powder, maltodextrin, Mel Powder, oligosaccharide add V-type blending tank and uniformly mix 25min, speed of agitator 20r/min, are subsequently adding modified dietary fiber and uniformly mix 12min, speed of agitator 40r/min, sterile filling, seal, pack and i.e. obtain pure natural blue berry composite powder；

Above-mentioned pure natural blue berry composite powder uses Aluminum-plastic composite bag fill；Disinfection by ultraviolet light is used before described Aluminum-plastic composite bag fill 20min。

The pure natural blue berry composite powder Lactobacillus plantarum viable bacteria content prepared through said method is 8.59 × 10¹¹cfu/g。

Embodiment four:

Blue berry powder 65 parts, Mel powder 27 parts, modified dietary fiber 13 parts, oligosaccharide 10 parts, 18 parts of Lactobacillus plantarum powder, Ferment powder 9 parts, maltodextrin 8 parts；

Wherein oligosaccharide parts by weight consist of oligofructose 50 parts, Oligomeric maltose 40 parts, Raffinose 40 parts, soy oligosaccharides Sugar 18 parts, oligomeric galactose 15 parts, oligomeric xylose 15 parts, oligomeric isomaltose 15 parts, 12 parts of stachyose；

Being put into by blue berry in the ultrasonic washing unit filling 0.3% sodium bicarbonate solution, 400W, 45KHz clean 10min at room temperature, Draining, each blue berry is arbitrarily cut into four parts, and single flat is layered in microwave dryer in power 5kW, temperature 130 DEG C, is dried 5s, is then immersed in 10min in the sericin peptide taken solution that mass percent concentration is 12%, takes out, vertical after-22 DEG C of freezing 50min I.e. pulverize, ground product particle diameter 0.5mm, be subsequently added into the water of ground product quality 3 times, be 5.5 with breast acid for adjusting pH value, in In electric field intensity 35kV/cm under room temperature, burst length 500 μ s, carry out high voltage pulse electric field processing under the conditions of pulse frequency 300Hz 30min；Then under the conditions of power 300W, microwave irradiation and extraction 20min is carried out in room temperature, simultaneously in power 300W, frequency 40KHz Under the conditions of carry out ultrasonic assistant extraction；Add the mixed enzyme of extracting solution quality 2.5%, in 50 DEG C of enzymolysis 50min, enzymolysis solution mistake Filter, filtrate reduced in volume, lyophilization to moisture are 8% i.e. to obtain blue berry powder；

Above-mentioned mixed enzyme is that cellulase, protease, pectase, tannase 4:3:3:2 in mass ratio uniformly mixes.

According to formula proportion, weigh each raw material, successively by ferment powder, Lactobacillus plantarum powder, blue berry powder, maltodextrin, Mel Powder, oligosaccharide add V-type blending tank and uniformly mix 35min, speed of agitator 40r/min, are subsequently adding modified dietary fiber and uniformly mix 18min, speed of agitator 60r/min, sterile filling, seal, pack and i.e. obtain pure natural blue berry composite powder；

Above-mentioned pure natural blue berry composite powder uses Aluminum-plastic composite bag fill；Disinfection by ultraviolet light is used before described Aluminum-plastic composite bag fill 40min。

The pure natural blue berry composite powder Lactobacillus plantarum viable bacteria content prepared through said method is 7.52 × 10¹¹cfu/g。

Embodiment five:

Blue berry powder 50 parts, Mel powder 20 parts, modified dietary fiber 8 parts, oligosaccharide 6 parts, 14 parts of Lactobacillus plantarum powder, ferment 5 parts of powder of element, maltodextrin 5 parts；

Wherein oligosaccharide is stachyose；

Being put into by blue berry in the ultrasonic washing unit filling 0.1% sodium bicarbonate solution, 400W, 35KHz clean 10min at room temperature, Draining, each blue berry is arbitrarily cut into four parts, and single flat is layered in microwave dryer in power 3kW, temperature 130 DEG C, is dried 3s, is then immersed in 8min in the sericin peptide taken solution that mass percent concentration is 12%, takes out, vertical after-22 DEG C of freezing 30min I.e. pulverize, ground product particle diameter 0.5mm, be subsequently added into the water of ground product quality 1 times, be 5.5 with breast acid for adjusting pH value, in In electric field intensity 25kV/cm under room temperature, burst length 500 μ s, carry out high voltage pulse electric field processing under the conditions of pulse frequency 200Hz 30min；Then under the conditions of power 150W, microwave irradiation and extraction 20min is carried out in room temperature, simultaneously in power 200W, frequency 40KHz Under the conditions of carry out ultrasonic assistant extraction；Add the mixed enzyme of extracting solution quality 1.5%, in 50 DEG C of enzymolysis 30min, enzymolysis solution mistake Filter, filtrate reduced in volume, lyophilization to moisture are 8% i.e. to obtain blue berry powder；

Above-mentioned mixed enzyme is that cellulase, protease, pectase, tannase 2:3:1:2 in mass ratio uniformly mixes.

According to formula proportion, weigh each raw material, successively by ferment powder, Lactobacillus plantarum powder, blue berry powder, maltodextrin, Mel Powder, oligosaccharide add V-type blending tank and uniformly mix 28min, speed of agitator 25r/min, are subsequently adding modified dietary fiber and uniformly mix 14min, speed of agitator 45r/min, sterile filling, seal, pack and i.e. obtain pure natural blue berry composite powder；

Above-mentioned pure natural blue berry composite powder uses Aluminum-plastic composite bag fill；Ozonization 20min is used before described Aluminum-plastic composite bag fill.

The pure natural blue berry composite powder Lactobacillus plantarum viable bacteria content prepared through said method is 6.84 × 10¹¹cfu/g。

Embodiment six:

Blue berry powder 70 parts, Mel powder 30 parts, modified dietary fiber 15 parts, oligosaccharide 12 parts, 20 parts of Lactobacillus plantarum powder, Ferment powder 10 parts, maltodextrin 10 parts；

Wherein oligosaccharide is oligomeric galactose；

Being put into by blue berry in the ultrasonic washing unit filling 0.3% sodium bicarbonate solution, 200W, 45KHz clean 5min at room temperature, Draining, each blue berry is arbitrarily cut into four parts, and single flat is layered in microwave dryer in power 5kW, temperature 120 DEG C, is dried 5s, is then immersed in 10min in the sericin peptide taken solution that mass percent concentration is 8%, takes out, vertical after-18 DEG C of freezing 50min I.e. pulverize, ground product particle diameter 0.3mm, be subsequently added into the water of ground product quality 3 times, be 3.5 with breast acid for adjusting pH value, in In electric field intensity 35kV/cm under room temperature, burst length 300 μ s, carry out high voltage pulse electric field processing under the conditions of pulse frequency 300Hz 20min；Then under the conditions of power 300W, microwave irradiation and extraction 15min is carried out in room temperature, simultaneously in power 300W, frequency 30KHz Under the conditions of carry out ultrasonic assistant extraction；Add the mixed enzyme of extracting solution quality 2.5%, in 40 DEG C of enzymolysis 50min, enzymolysis solution mistake Filter, filtrate reduced in volume, lyophilization to moisture are 5% i.e. to obtain blue berry powder；

Above-mentioned mixed enzyme is that cellulase, protease, pectase, tannase 4:1:3:1 in mass ratio uniformly mixes.

According to formula proportion, weigh each raw material, successively by ferment powder, Lactobacillus plantarum powder, blue berry powder, maltodextrin, Mel Powder, oligosaccharide add V-type blending tank and uniformly mix 33min, speed of agitator 35r/min, are subsequently adding modified dietary fiber and uniformly mix 17min, speed of agitator 55r/min, sterile filling, seal, pack and i.e. obtain pure natural blue berry composite powder；

Above-mentioned pure natural blue berry composite powder uses Aluminum-plastic composite bag fill；Ozonization 40min is used before described Aluminum-plastic composite bag fill.

The pure natural blue berry composite powder Lactobacillus plantarum viable bacteria content prepared through said method is 6.96 × 10¹¹cfu/g。

Experimental example seven eats the change of T-CHOL after pure natural blue berry composite powder

Selecting the adult 48 of T-CHOL 180mg/dl-250mg/dl, men and women half and half, is randomly divided into three groups；First group of every day 150 milliliters of mineral waters are drunk in dinner；Second group of common commercially available blue berry composite powder 150g of dinner every day Instant Drinks, the 3rd group of dinner every day The blue berry composite powder 150g of the Instant Drinks embodiment of the present invention 2, eats same food every day period, and food includes meat, egg, vegetable And fruit.Gather the blood of experimenter respectively in the previous day and the 20th, 40,60 days that experiment starts, measure in blood is total Cholesterol level, result such as table 1:

Table 1: total cholesterol level testing result in blood

Time	0 day	20 days	40 days	60 days
					First group (mg/dl)	202.3	203.8	206.2	209.3
Second group (mg/dl)	207.6	206.5	203.2	202.1
					3rd group (mg/dl)	210.8	203.9	193.5	171.1

As seen from the above table after the blue berry composite powder of the Instant Drinks embodiment of the present invention 2, the content of the T-CHOL in adult's blood occurs Significant change.Compared with common commercially available blue berry composite powder, blue berry composite powder of the present invention containing the T-CHOL in adult's blood Amount significantly reduces, and the content of the T-CHOL in mineral water composition year human blood significantly increases, though commercially available blue berry composite powder So decrease, but compared with product of the present invention, effect is notable, it follows that the present invention uses has reduction cholesterol spy Property the blue berry composite powder prepared of specific bacterial strain there is the health-care effect well reducing cholesterol.

It should be understood that the pure natural blue berry composite powder prepared by embodiment of the present invention 3-6 has above-mentioned technique effect equally, Between each embodiment, diversity is the most notable.

Embodiment eight pure natural of the present invention blue berry composite powder shelf-life implants living preparation of lactobacillus stable content is tested

The blue berry composite powder taking the embodiment of the present invention 2 preparation stores 3 months, 6 under room temperature 22-25 DEG C, ventilation condition respectively The moon, 12 months, 24 months, 36 months also measure Lactobacillus plantarum viable bacteria content, result such as table 2:

Table 2: shelf-life implants living preparation of lactobacillus content detection result

Result above shows: the storage stability of blue berry composite powder of the present invention is preferable, environment resistant (temperature, appropriateness, oxygen, light According to, moisture etc.) influence factor's ability is big, and shelf-life Lactobacillus plantarum viable bacteria content loss rate maximum (36 months) is 15%, Higher than existing like product viable bacteria content, loss rate is low, long shelf-life, reflect that other of blue berry composite powder is biological simultaneously and live Property composition has same storage stability.

It should be understood that pure natural blue berry composite powder prepared by embodiment of the present invention 3-6 has above-mentioned experiment effect, respectively equally Between embodiment and little with above-mentioned experiment effect diversity.

The sensory evaluation test of embodiment nine pure natural of the present invention blue berry composite powder

Invite the blue berry of blue berry composite powder that the embodiment of the present invention 2 prepared by 24 personnel and commercially available two kinds of similar identical dates of manufacture Composite powder is judged, and sense organ is given a mark, wherein specialty and each 12 of layman, and professional is young, middle aged, old each 4, men and women half and half, and layman is juvenile, young, middle aged, each 3 of old age, and men and women half and half；Marking includes outward appearance (20 Point), quality (25 points), local flavor (30 points), four aspects of mouthfeel (25 points), marking personnel independently carry out, are independent of each other, Result is judged accurate with guarantee.Being added up judging result, equal score value takes approximation, retains integer, is specifically shown in Table 3:

Table 3: sensory evaluation statistical result

Note: show significant difference (P ＜ 0.05) with a line internal standard difference lowercase alphabet, the different capitalization of mark represents that difference is extremely notable (P ＜ 0.01), Indicate same letter and represent that difference is not notable (P ＞ 0.05).

Result above shows, blue berry composite powder prepared by the present invention from outward appearance, quality, local flavor and mouthfeel any one will be obvious It is better than commercially available blue berry composite powder, particularly outward appearance, local flavor and mouthfeel fabulous, also is adapted for different age group, different consumption layer simultaneously Secondary consumer eats.

The embodiment ten pure natural of the present invention probiotic test of blue berry composite powder

The blue berry composite powder embodiment of the present invention 2 prepared, being prepared as viable count with sterilized water is 2 × 10¹⁰The plant breast of CFU/mL Bacillus solution, saves backup in 4 DEG C；

1, the 10mL Lactobacillus plantarum solution kept of going bail for is injected in test tube 1, uses ten times of stepwise dilutions to 10^-8, take 1mL dilute Release liquid on flat board, the MRS agar culture medium being cooled to 45 DEG C is poured on flat board (sterilizing), shake up rapidly after sterilizing.Again The test tube 2 that will be equipped with 10mL Lactobacillus plantarum solution is placed in 80-90 DEG C of water-bath heating 15-25min, takes the breast of the plant after heating Bacillus solution carries out ten times of stepwise dilutions to 10^-8, take 1mL diluent on flat board, the MRS agar training of 45 DEG C after sterilizing, will be cooled to Foster base pours into upper at flat board (sterilizing) and shakes up rapidly.Finally the flat board before heating and after heating is all cultivated under the conditions of 35 DEG C 24h, calculates the quantity before and after heating.

Result shows, Viable detection has reached more than 88%.

2, simulated gastric fluid and the resistance test of intestinal juice: the hydrochloric acid 16.4mL taking 100g/L adds distilled water diluting, makes pH value be respectively 1.5,2.5 and 3.5, take 100mL dilute hydrochloric acid solution, be separately added into 1g pepsin so that it is fully dissolve, obtain simulated gastric fluid, Microporous filter membrane degerming (0.22 μm) is standby.Taking potassium dihydrogen phosphate 6.8g, the 500mL that adds water makes dissolving, uses 0.1moL/L hydroxide Sodium solution regulation pH value is to 6.8；Separately taking trypsin 10g, the 100mL that adds water makes dissolving, after two liquid mixing, is diluted with water to 1000ml, obtains simulated intestinal fluid, and microporous filter membrane degerming (0.22 μm) is standby.Take the Lactobacillus plantarum solution addition that 1mL keeps In the simulated gastric fluid of 9mL (i.e. ten times stepwise dilutions), and the most fully mix rapidly, be subsequently placed in 30-45 DEG C quiet Put cultivation 2-4h.1h, 2h, 3h, 4h when, take out culture fluid respectively and count remaining viable count immediately, with former viable count Comparing, result shows, Viable detection is 98%.Then each 1mL of culture fluid digesting different time it is taken in simulated gastric fluid, Be inoculated in respectively in the simulated intestinal fluid that 9mL pH value is 6.8, be placed in 30-45 DEG C of quiescent culture 2-4h, and respectively 0,3,6,24h Sampling, measures its viable count, compares with former viable count, and result shows that Viable detection is 99%.

3, the resistance test of cholate is simulated: make the solution of 1g/L, and the Fel Sus domestica salt of addition 0.8% in the solution with pancreatin, It is 8.0 that NaOH with 10% adjusts pH, then with 0.45 μm micro-filtrate membrane filtration degerming.The Lactobacillus plantarum that 0.5mL is kept Solution inoculum is simulated in cholate to 4.5mL, obtains culture fluid, the viable count of counting remaining after cultivating 24h.By culture fluid in sterilizing In normal saline, ten times of stepwise dilutions are to 10^-8, and pour into MRS, it is subsequently placed in 35 DEG C of quiescent culture 24h.Result shows that viable bacteria is deposited Motility rate is 99%.

Above result of the test shows, Lactobacillus plantarum probiotic (thermostability, resistance to pH, the resistance to gallbladder in blue berry composite powder of the present invention Salt) relatively strong, it is especially suitable for human intestines and stomach's environment, in simulated gastrointestinal environments, survival rate is big, can be effectively improved gastrointestinal function.

Embodiment 11 pure natural of the present invention blue berry composite powder mouse intestinal performance test

Choosing common Kunming white mice 60, male and female half and half, 18-20g, conventional word is supported.Therefrom random choose 40, every day is early 9:00 gavage lincomycin hydrochloride 0.2mL in morning (20mg)/only, other as a control group, the gavage equivalent sterilizing of every day same time Normal saline, continuous one week, the mouse model of preparation alteration of intestinal flora.Model group mouse diet declines, do not occur dead and Significantly suffering from diarrhoea phenomenon, arrange soft excrement, profile normal aqueous divides more, and bedding and padding are moist.By 40 alteration of intestinal flora mices, with Machine is divided into 2 groups, and one group 20 is only used as treatment group, the Lactobacillus plantarum solution 0.5ml (2 × 10 that every day, gavage was kept¹⁰cfu/ml) / only, another 20 are only used as natural recovering group, every day same time gavage equivalent sterile saline, continuous two weeks.Whole experimental period 21 days, observe growth and the defecation situation of white mice every day, in the 8th, 21 days to blue berry composite powder treatment group and natural recovering group Mice is weighed, and calculates each group of weight average rate of increase, result such as table 4；Within every 5 days, survey each group of stool in mice escherichia coli quantity, Calculate average, result such as table 5.Take stool in mice about 0.1g, in aseptic operating platform, add 3 beades (with 0.1g excrement sample Add 0.5mL diluent), dilute and inoculate maconkey agar culture medium, calculate every gram wet just in coliform count.

Table 4: mice Gain weight

Packet	Average starting weight (g/ is only)	Average end weight (g/ is only)	Average rate of increase (%)
				Natural recovering group	20.69±1.33	27.34±1.59	32.14^a
Treatment group	20.41±1.45	34.28±1.62	67.96^b

Table 5: the situation of coliform count in stool in mice

Blue berry composite powder treatment group Mouse Weight average rate of increase (67.96%) is significantly higher than natural recovering group (32.14%)；Raise Feeding solution rear intestinal escherichia coli quantity to be remarkably decreased, reduce by 97.07%, substantially less than natural recovering group (24.78%), shows Lactobacillus plantarum in blue berry composite powder of the present invention is rapid field planting in white mice intestinal, forms dominant microflora, and effectively suppression is big The growth and breeding of the pathogen such as enterobacteria, and resident time is long, continues, effectively improves intestinal performance.

The impact on immunity of organisms of the embodiment 12 pure natural of the present invention blue berry composite powder

1 experiment purpose

Test (mouse forced swimming) by exercise tolerance, verify the raising immunity of blue berry composite powder of the present invention, antifatigue effect.

2 experiment materials and reagent

2.1 for reagent thing:

Commercially available blue berry composite powder (G1)；Commercially available blue berry composite powder (G2)；Blue berry composite powder prepared by embodiment of the present invention 2-6 (G3-G7)。

2.2 reagent:

Liver/muscle glycogen testing cassete, builds up institute of biological products purchased from Nanjing；Concentrated sulphuric acid (AR), the limited public affairs of Nanjing chemical reagent Department；Normal saline, Shangdong Changfu Jiejing Pharmaceutical Industry Co., Ltd..

3. laboratory animal

ICR mice, ♂, cleaning grade, body weight 18-22g, Ningxia Medical University's comparative medicine center provide, little during experiment The free diet of Mus.

4. key instrument

Aluminum swimming trunk (50cm × 50cm × 40cm), galvanized wire, low-temperature and high-speed centrifuge: 5804R type, Eppendrof is public Department；Water-bath: DK-S26 type, upper Nereid grand experimental facilities company limited；Electronic scale: BS224S type, Sartorius Company；Stopwatch, thermometer

5. experiment packet

5.1 dosage packets and given the test agent give the time and at random mice are divided into 8 groups, and often group 10, the 1st group to the 7th component Not Gei the medicine of G1～G7, the 8th group is blank group, gives isopyknic distilled water, the often every average daily gavage of group 1 time, Gavage volume is 0.2ml/10g, gives given the test agent continuously 30 days.

5.2 sample preparations the 1st group are to the 7th group: weigh 2.25g drug sample, be assigned to 150ml with distilled water；Blank Group: distilled water 150ml.

6. experimental technique

6.1 swimmings with a load attached to the body experiment lasts are administered after 30min, put mice in swimming trunk, and the depth of water is no less than 30cm, water temperature 25 ± 1 DEG C, the sheet lead of rat-tail root load 5% body weight, record mice swimming starts to the dead time, when swimming as mice Between.

After 6.2 mice serum carbamide measure last administration 30min, not swimming with a load attached to the body 90min in the water that temperature is 30 DEG C, Pluck after rest 60min eyeball blood sampling 0.5mL (being not added with anticoagulant), put 4 DEG C of refrigerator 3h, after hemopexis 2000r/min from Heart 15min, takes serum and send clinical laboratory of Affiliated Hospital of Ningxia Medical University to detect.

After the mensuration last of 6.3 hepatic glycogen is administered 30min, not swimming with a load attached to the body 90min in the water that temperature is 25 ± 1 DEG C, cervical vertebra Mice is put to death in dislocation, cleans with normal saline, and with after filter paper suck dry moisture, accurately weighs liver 100mg, and hepatic glycogen detects Test kit detection Mouse Liver glycogen content.

The mensuration last of 6.4 blood lactase acid is taken a blood sample after being administered 30min, does not the most bear a heavy burden at the water went swimming 10min that temperature is 30 DEG C Rear stopping.Lactic acid instrument assay method: respectively before swimming, each blood sampling 20 μ L add after rest 20min after swimming, after swimming Entering in 40 μ L rupture of membranes liquid, the most fully vibration smudge cells lactic acid instrument measures.(blood lactase acid area under curve=5 × (swimming The blood lactase acid value of 20min after blood lactase acid value+2 × swimming of 0min after front blood lactase acid value+3 × swimming)

7. observation index walking weight load, blood lactase acid, carbamide, glycogen initial value

8. statistical method experimental data is usedRepresent, use t inspection to compare between organizing

9. experimental result

The impact on Mouse Weight of the 9.1 blue berry composite powders of the present invention

Each group mice after giving G1～G9 medicine, front, in, shown in post-weight see table respectively, at the beginning of each group mice Initial body weight and weightening finish body weight compare equal no difference of science of statistics (P ＞ 0.05) with matched group, show that G1～G9 medicine is all without significantly Toxicity.Experimental result refers to table 6.

The original body mass of table 6 swimming with a load attached to the body experiment mice, body weight in mid-term and end body weight

The impact on the mice burden swimming time of the 9.2 blue berry composite powders of the present invention

After per os gives mice G1～G7 medicine, G1～G2 medicine compares with blank group, can be obviously prolonged mice and bear Weight swimming time, has significant difference (P ＜ 0.05), blue berry composite powder G3 of the present invention～G7 medicine and blank group ratio Relatively, can significantly extend the mice burden swimming time, there is pole significant difference (P ＜ 0.01), and be substantially better than G1～G2 Medicine.The results detailed in Table 7.

The impact on the mice burden swimming time of the table 7 blue berry composite powder

" * " p < 0.05vs blank；

" * * " p < 0.01vs blank；

9.3 blue berry composite powders of the present invention are on the impact of blood lactase acid before and after mouse movement

After per os gives the blue berry composite powder of the mice present invention, blue berry composite powder G3 of the present invention～G7 medicine are to blood after mouse movement Lactic acid area under curve compares with matched group significant difference (P ＜ 0.05), under G1～G2 medicine group Mouse Blood lactic acid curve Decrease though area compares with matched group, but and no difference of science of statistics (P ＞ 0.05).The results are shown in Table 8.

Table 8 blue berry of the present invention composite powder is on the impact of blood lactase acid level before and after mouse movement

" * " p < 0.05vs blank；

The impact on Mouse Liver glycogen of the 9.4 blue berry composite powders of the present invention

After per os gives mice G1～G7 medicine, G1～G2 medicine compares with blank group, and Mouse Liver glycogen content all has bright Aobvious rising, have significant difference (P ＜ 0.05), blue berry composite powder G3 of the present invention～G7 medicine with blank group Relatively, Mouse Liver glycogen content all has significantly rising, has a pole significant difference (P ＜ 0.01), and be substantially better than G1～ G2 medicine.The results detailed in Table 9.

The impact on Mouse Liver glycogen content of the table 9 blue berry of the present invention composite powder

" * " p < 0.05vs blank；

" * * " p < 0.01vs blank；

The impact on mice serum carbamide of the 9.5 blue berry composite powders of the present invention

After per os gives mice G1～G7 medicine, G1～G2 medicine group compares with blank group, serum urea after mouse movement Content all has significantly reduction, have significant difference (P ＜ 0.05), blue berry composite powder G3 of the present invention～G7 medicine with sky White matched group compares, and after mouse movement, serum urea content all has significantly reduction, has pole significant difference (P ＜ 0.01), And it is substantially better than G1～G2 medicine.The results detailed in Table 10.

The impact on mice serum urea content of the table 10 blue berry of the present invention composite powder

" * " p < 0.05vs blank；

" * * " p < 0.01vs blank；

10. experiment conclusion

This experiment is mainly tested by mice burden swimming, and the deposit of detection Mouse Liver glycogen observes blue berry composite powder of the present invention simultaneously Improve immunity, the effect of resisting fatigue.Preliminary Results shows as follows:

1, G3～G7 blue berry composite powder of the present invention all can extend the mice burden swimming time (P ＜ 0.01), and effect is the most excellent Blue berry composite powder in other G1～G2.

2, biochemistry detection aspect shows, G3～G7 blue berry composite powder of the present invention each dosage group all can reduce move after in mice serum Lactic acid content produced by glucose anerobic glycolysis, compares with matched group and has significant difference (P ＜ 0.05), and other G1～ Although the blue berry composite powder of G2 also can reduce after motion lactic acid content produced by glucose anerobic glycolysis in mice serum, but with right Compare according to group, no difference of science of statistics (P ＞ 0.05)；

3, G3～G7 blue berry composite powder of the present invention each dosage group all can significantly improve the deposit (P ＜ 0.01) of glycogen in mouse liver, And effect is substantially better than the blue berry composite powder of other G1～G2；

4, metabolic arthritis model finds, G3～G7 blue berry composite powder of the present invention can significantly reduce the containing of urea in serum after mice swimming Measure (P ＜ 0.01), and effect is substantially better than other G1～G2 blue berry composite powder；

11. conclusions

Above-mentioned experiment proves that pure natural blue berry composite powder of the present invention can significantly improve immunity of organisms, improves muscle power and the endurance of mice, Reduce urea in serum and the content of lactic acid after mouse movement, and the deposit of glycogen in mouse liver can be significantly improved, contribute to delaying Solve the fatigue that sports load causes；The time that mice burden swimming to power exhausts can be extended.

Claims

1. a pure natural blue berry composite powder, is mainly prepared by the raw material of following parts by weight: blue berry powder 50-70 part, Mel powder 20-30 Part, modified dietary fiber 8-15 part, oligosaccharide 6-12 part, Lactobacillus plantarum powder 14-20 part, ferment powder 5-10 part, Fructus Hordei Germinatus Dextrin 5-10 part；

Described Lactobacillus plantarum powder is to prepare according to a conventional method with Lactobacillus plantarum CGMCC NO.11763 for starting strain.

2. pure natural blue berry composite powder as claimed in claim 1, it is characterised in that mainly prepared by the raw material of following parts by weight: Blue berry powder 55-65 part, Mel powder 23-27 part, modified dietary fiber 11-13 part, oligosaccharide 8-10 part, Lactobacillus plantarum powder 16-18 part, ferment powder 7-9 part, maltodextrin 6-8 part.

3. pure natural blue berry composite powder as claimed in claim 1, it is characterised in that mainly prepared by the raw material of following parts by weight: Blue berry powder 60 parts, Mel powder 25 parts, modified dietary fiber 12 parts, oligosaccharide 9 parts, 17 parts of Lactobacillus plantarum powder, ferment 8 parts of powder, maltodextrin 7 parts.

4. the pure natural blue berry composite powder as described in claim 1-3 is arbitrary, it is characterised in that the preparation method of described blue berry powder, including Following steps, put in the ultrasonic washing unit filling 0.1-0.3% sodium bicarbonate solution by blue berry, at room temperature 200-400W, 35-45KHz cleans 5-10min, drains, and each blue berry is arbitrarily cut into four parts, and single flat is layered in microwave dryer in merit Rate 3-5kW, temperature 120-130 DEG C, dry 3-5s, be then immersed in the sericin peptide taken solution that mass percent concentration is 8-12% 8-10min, takes out, pulverizes immediately, ground product particle diameter 0.3-0.5mm, then add after-18--22 DEG C of freezing 30-50min Enter the water of ground product quality 1-3 times, be 3.5-5.5 with breast acid for adjusting pH value, at room temperature in electric field intensity 25-35kV/cm, Burst length 300-500 μ s, carries out high voltage pulse electric field processing 20-30min under the conditions of pulse frequency 200-300Hz；Then in room Temperature carries out microwave irradiation and extraction 15-20min under the conditions of power 150-300W, simultaneously in power 200-300W, frequency 30-40KHz Under the conditions of carry out ultrasonic assistant extraction；Add the mixed enzyme of extracting solution quality 1.5-2.5%, in 40-50 DEG C of enzymolysis 30-50min, Enzymolysis solution filters, and filtrate reduced in volume, lyophilization to moisture are that 5-8% i.e. obtains blue berry powder；

5. the pure natural blue berry composite powder as described in claim 1-3 is arbitrary, it is characterised in that the preparation method of described Mel powder, bag Include following steps: uniformly mixed by Mel, maltodextrin, beta-schardinger dextrin-5-7:2-4:0.5-1.5 in mass ratio, add 50 DEG C Deionized water, fully dissolve so that the solid content of mixed liquor is 32-35%, and mixed liquor is in power 200-400W, frequency Ultrasonic Treatment 5-10min is carried out under 30-40KHz, room temperature condition, then through colloid mill circular grinding 2-4 time, homogenizing, homogenizing Pressure 25-30MPa, homogenizing temperature 50-60 DEG C, the spray-dried machine of homogenizing fluid is in inlet temperature 180-200 DEG C, leaving air temp 70-90 DEG C is drying to obtain Mel powder, and Mel powder particle diameter is 20-40 mesh.

6. the pure natural blue berry composite powder as described in claim 1-3 is arbitrary, it is characterised in that described ferment powder preparation method is as follows: The big grain materials such as the fermentation liquid using pickle fermentation to produce discharge in latter stage is raw material, the dish leaf being filtered to remove in fermentation liquid are fermented Liquid, fermented liquid uses lyophilization to obtain ferment powder；Cryodesiccated condition is :-30--40 degree pre-freeze 6 hours；So Rear lyophilizing is until water content is less than 5%.

7. pure natural blue berry composite powder as claimed in claim 1, it is characterised in that cryoprotective agent when preparing Lactobacillus plantarum powder Preparation method, comprises the steps: winter rye, Caulis et Folium Ammopiptanthi Mongolici, sharkskin collagen protein are respectively washed, are drained, in mass ratio 8-10:3-5:2-4 uniformly mixes, and adds the lactic acid moistening 3-8h that pH value is 3.8-4.5 of mixed material quality 0.1-1 times, Pulverize immediately after-18--22 DEG C of freezing 1-2h, freezing thickness of feed layer 3-5cm, ground product particle diameter 0.5-3mm, then add Enter the water of ground product quality 10-20 times, be 3.5-5.5 with breast acid for adjusting pH value, at room temperature in electric field intensity 25-35kV/cm, Burst length 300-500 μ s, carries out high voltage pulse electric field processing 20-30min under the conditions of pulse frequency 200-300Hz；Then in room Temperature carries out microwave irradiation and extraction 15-20min under the conditions of power 150-300W, simultaneously in power 200-300W, frequency 30-40KHz Under the conditions of carry out ultrasonic assistant extraction；Add the compound enzyme of extracting solution quality 1-2%, in 45-55 DEG C of enzymolysis 30-50min；Enzyme Solve liquid filtration, filtrate concentrates, low-temperature grinding to particle diameter is that 0.1-0.3mm i.e. obtains protective agent；

Described compound enzyme is cellulase, protease, amylase, pectase, tannase 2-4:1-3:1-3:1-2:1-2 in mass ratio Uniformly mixing.

8. the pure natural blue berry composite powder as described in claim 1-3 is arbitrary, it is characterised in that the preparation side of described modified dietary fiber Method, comprises the following steps: is uniformly mixed by inulin, Semen Tritici aestivi fiber, Herba avenae fatuae fiber 5-7:2-4:1-3 in mass ratio, adds it The water of quality 3-7 times, room temperature 100-300W, 35-40KHz condition supersound extraction 10-15min, then in electric field intensity 20-40kV/cm, burst length 300-500 μ s, carry out high voltage pulse electric field processing under the conditions of pulse frequency 200-400Hz 10-15min；It is 4.5-6.5 with breast acid for adjusting pH value, adds the enzyme of mixture quality 0.1-0.3%, in 45-55 DEG C of enzyme Solve 20-48min；Enzymolysis solution filters, and filtrate reduced in volume, lyophilization to moisture are that 5-8% i.e. obtains modified dietary fiber；

9. the preparation method of pure natural blue berry composite powder as described in claim 1-8 is arbitrary, it is characterised in that comprise the steps: by According to formula proportion, weigh each raw material, successively by ferment powder, Lactobacillus plantarum powder, blue berry powder, maltodextrin, Mel powder, low Polysaccharide adds V-type blending tank and uniformly mixes 25-35min, speed of agitator 20-40r/min, is subsequently adding modified dietary fiber and uniformly mixes Close 12-18min, speed of agitator 40-60r/min, sterile filling, seal, pack and i.e. obtain pure natural blue berry composite powder.

10. the preparation method of pure natural blue berry composite powder as described in claim 9 is arbitrary, it is characterised in that described oligosaccharide weight portion Array becomes oligofructose 40-50 part, Oligomeric maltose 30-40 part, Raffinose 20-40 part, soybean oligo saccharide 16-18 part, Oligomeric galactose 10-15 part, oligomeric xylose 10-15 part, oligomeric isomaltose 10-15 part, stachyose 8-12 part.