CN105661247A

CN105661247A - Grape composite powder and preparation method thereof

Info

Publication number: CN105661247A
Application number: CN201610074646.3A
Authority: CN
Inventors: 庞美华; 龚利芸
Original assignee: Yi Jian (dalian) Biotechnology Development Co Ltd
Current assignee: Yi Jian (dalian) Biotechnology Development Co Ltd
Priority date: 2016-02-02
Filing date: 2016-02-02
Publication date: 2016-06-15

Abstract

The invention discloses a grape composite powder and a preparation method thereof. The grape composite powder is prepared from grape powder with natural color and flavor, lactobacillus plantarum powder, wheat malt powder, modified dietary fiber, enzyme powder, papaya powder and other functional products through scientific compounding, the nutrition, the food safety and the health care function of the grape composite powder are improved, and the probiotic property and the quality stability of the grape composite powder are remarkably improved. Compared with existing grape composite powder, the grape composite powder contains simple components and has natural color, flavor and taste, high nutritional value and a good healthcare function, the content of bioactive substance is high, the number of viable bacteria of probiotics is high, the probiotic property is good, the functional metabolin variety of probiotics is complete, the content is high, the food safety is high, and the guarantee period is long.

Description

A kind of Fructus Vitis viniferae composite powder and preparation method thereof

Technical field

The present invention relates to Fructus Vitis viniferae deep processed product, be specifically related to a kind of Fructus Vitis viniferae composite powder and preparation method thereof.

Background technology

Solid beverage be moisture within 5%, there is definite shape, for instance graininess, lamellar, bulk, Powdered etc., need to through the dissolved beverage just can drunk. Solid beverage is removed moisture by liquid beverage and makes, and removes the purpose of moisture: one is prevent the enzyme of beverage itself or microbial deterioration or corruption, in order to storage; Two are easy for storing and transport. Compared with liquid beverage, solid beverage has a characteristic that quality significantly alleviates, and volume significantly diminishes, easy to carry; Raciness, instant capacity is good, applied range, drinks conveniently; It is prone to keep health; Pack simple and easy, convenient transportation. Solid beverage, based on certain raw material, is equipped with what other adjuvant processed, by the classification of its primary raw material, solid beverage can be divided into Fruity type, protein type and three kinds of other type. They will add sugar or sweeting agent. Prior art often replaces some or all of sucrose with some sweeting agent. However as increasing of auxotype disease, solid beverage, as: in solid milk tea, coffee creamer, bean milk powder, sesame paste, walnut powder etc., the problem starting to need to add composite sweetener low in calories to solve energy and overnutrition.

Resveratrol is polyphenol compound, is mainly derived from the plants such as Fructus Vitis viniferae (red wine), Rhizoma Polygoni Cuspidati, Semen arachidis hypogaeae, Fructus Mori. Resveratrol is a kind of biological very strong natural polyphenol class material, is also called resvertrol, is the chemopreventive agent of tumor, is also to reducing platelet aggregation, the chemopreventive agent of prevention and treatment atherosclerosis, cardiovascular and cerebrovascular disease. The result of study of United States Department of Agriculture (USDA) shows, also contains considerable resveratrol in Testa arachidis hypogaeae and core. The experimentation of resveratrol it turned out the beneficial effect having cardiovascular disease and cancer. Hormone-dependent tumor is had obvious preventive effect by resveratrol. Also osteoporosis, acne and senile dementia can there be is preventive effect, there is antiviral and immunoregulation effect. Inside of human body one monomer defying age enzyme is worked, and then plays the latent effect preventing various age-related disease, extending life expectancy. Resveratrol has health-care effect very widely, such as Frenchman often drinks in higher fatty acid thing containing resveratrol, so French Incidence of CHD is lower than other western countries, just because of resveratrol is because high-fat preventive and therapeutic effect can be suppressed effectively, existing Countries and area develop resveratrol using resveratrol and goods thereof as dietary supplement.

At present with Fructus Vitis viniferae for raw material, prepare the less of pure grape fruit powder:

Chinese patent CN102885265A discloses the manufacture method of a kind of grape fruit powder. The present invention is with fresh Fructus Vitis viniferae for raw material, after screening, heating carries out enzyme denaturing process, then in zinc acetate solution and ascorbic acid solution, carry out color fixative, then dry, finally by process after Fructus Vitis viniferae pulverize and sieve, vacuum packaging, sterilizing, obtain grape fruit powder finished product, this product color is bright-coloured, nutritious, long shelf-life. The present invention has the advantages such as technique is simple, easy to operate, production cost is low, is suitable for industrialized production. Patent color preservation technology tradition disclosed above, weak effect, introduce chemistry colour fixative, foodsafety is poor, adopts heating enzyme denaturing and drying, bioactive substance and heat-sensitive ingredients loss big.

With Fructus Vitis viniferae for primary raw material, prepare the more of Fructus Vitis viniferae composite powder:

Chinese patent CN104172005A discloses a kind of prebiotics Fructus Vitis viniferae blue berry composite fruit powder and preparation method thereof, is mainly made up of following raw material: grape 50～80 parts, blueberry fresh fruit 20～50 parts, oligofructose 2～5 parts. Manufacture method is as follows: (1) takes grape and blueberry fresh fruit is cleaned; (2) crush and the edible sulfurous acid of addition obtains pulp in shattering process; (3) take the pulp of step (2), add pectase; (4) take the pulp after enzymolysis and squeeze to obtain blended fruit juice; (5) oligofructose is added; (6) soluble solid content is concentrated into 25%; (7) filter; (8) lyophilization; (9) sieve to obtain fruit powder finished product. The product that the present invention relates to, rich in compositions such as anthocyanidin, organic acid, vitamin, has significantly high nutritive value, quickly dissolving, can be used for the raw material of the food such as beverage, cake. Patent disclosed above adopts traditional juice preparation method nutrient component damages big, and recovery rate is low, and health-care effect is undesirable.

Chinese patent CN104970419A discloses a kind of grape solids beverage, each component including following weight/mass percentage composition: Semen Vitis viniferae powder 20～25%, Fructus Pruni pseudocerasi dry powder 18～22%, blue berry dry powder 15～18%, Fructus psidii guajavae immaturus powder 15～17%, bacillus bifidus 3～5%, Protamax 4～6%, soy bean edible fiber powder 5～10%, vitamin 2～4% and composite sweetener 2～5%. Grape solids beverage provided by the present invention has the advantage that the metabolism promoting body, strong body constitution; Cleaning intestinal rubbish, toxin expelling purify; Green natural fat reducing weight reducing etc. The composite sweetener added in beverage reduces the disagreeable taste that Sucus Vitis viniferae solid drink itself is existing so that solid beverage sugariness is soft, is not result in dental caries, raises without causing blood glucose cholesterol. Patent functional materials disclosed above is Semen Vitis viniferae, and raw material availability is low, cost is high, and food additive is many, and safety is low.

The also more of fruit and vegerable composite powder is prepared for adjuvant with Fructus Vitis viniferae:

Chinese patent CN102940214B discloses a kind of vegetables and fruits fiber nutrition powder, and described vegetables and fruits powder processing method is as follows: (1) pre-treatment: take fruit and vegetable materials, cleans, removes the peel, pulls an oar, it is thus achieved that fruit and vegerable serosity; (2) dried: take concentrated solution spray drying, obtains described fruit vegetable powder; (3) allotment: proportionally add; (4): packaging. Described fruit vegetable powder is made by the raw material of following weight fraction is composite: Plant fiber: 20 parts, plant colloid: 15 parts, probiotics factor: 10 parts, aminoacids complex 5 parts, ribonucleic acid: 0.6 part, Wheat Sprout Powder: 6 parts, ferment: 3 parts, malt extract: 10 parts, unsaturated fatty acid: 1 part, Guarana extract: 1 part, egg albumen powder: 8 parts, flavoring agent: 0.03 part, compound vitamin and mineral: 0.6 part, 10 parts of vegetables and fruits powder.Vegetables and fruits powder features good taste of the present invention, moisture is low easily stored, and high nutrition is low in calories, has good eliminating toxin and beautifying the skin effect, has lifting sleep quality simultaneously, weakens the effect of anxiety. Patent complicated components disclosed above, material treatment process is simple, and active substance loss is big, and health-care effect is still undesirable.

Chinese patent CN104286756A discloses a kind of fat-reducing fruit vegetable powder, with there is antiobesity action natural plants for raw material, adopt low-temperature extraction and biological enzymolysis technology, remain effective ingredient therein to greatest extent, the composite dietary fiber of science on the basis of existing fruit-vegetable nutrition powder, plant extract, Folium Camelliae sinensis extract, spice extract, Chinese herbal medicine extract, probiotic bacteria powder, the fat-reducing effect such as probiotics factor and bamboo charcoal powder is preferably lost weight the factor, simultaneously also composite egg albumen powder, malt extract, Wheat Sprout Powders etc. have the nutrient substance of antiobesity action, and with the addition of aminoacids complex, the nutrient such as vitamin and mineral, mainly from meeting satiety, regulate Biology Barrier of Gastrointestinal Tract, Traditional Chinese medicine health-preserving, the multi-angles such as liposuction row's fat are set out, maintain and improve the digestion of human body self regulation and the level absorbed and ability, decreasing picked-up and the secondary synthesis approach of body fat, the nutrient substance without influence on human normal absorbs and metaboilic level, it is achieved the benign cycle of health preserving fat-reducing. patent complicated components disclosed above, probiotic bacteria adopts tradition strain, and functional health effect is still undesirable.

To sum up, with Fructus Vitis viniferae for primary raw material, a kind of mouthfeel of preparation and color and luster are natural, and nutritional labeling and bioactive substance content are high, and the component significant Fructus Vitis viniferae composite powder of strong health-care effect simple, functional is still a kind of necessary.

Summary of the invention

Solved by the invention technical problem is that the defect overcoming existing Fructus Vitis viniferae composite powder and preparation thereof, with grape fruit powder for primary raw material, add probiotic powerful Lactobacillus plantarum powder, ferment powder, and a kind of mouthfeel of the functional raw material such as the composite Wheat Sprout Powder of science, modified dietary fiber preparation and color and luster natural, nutritional labeling and bioactive substance content are high, component strong, the significant Fructus Vitis viniferae composite powder of health-care effect simple, functional.

In order to achieve the above object, the present invention is by the following technical solutions:

A kind of Fructus Vitis viniferae composite powder, is mainly prepared by the raw material of following parts by weight:

Grape fruit powder 30-50 part, Wheat Sprout Powder 20-30 part, modified dietary fiber 8-15 part, papaya powder 6-12 part, Lactobacillus plantarum powder 6-12 part, ferment powder 5-10 part, Fructus Citri Limoniae powder 5-10 part;

Preferably, described Fructus Vitis viniferae composite powder, mainly prepared by the raw material of following parts by weight:

Grape fruit powder 35-45 part, Wheat Sprout Powder 23-27 part, modified dietary fiber 11-13 part, papaya powder 8-10 part, Lactobacillus plantarum powder 8-10 part, ferment powder 7-9 part, Fructus Citri Limoniae powder 6-8 part;

It is highly preferred that described Fructus Vitis viniferae composite powder, mainly prepared by the raw material of following parts by weight:

Grape fruit powder 40 parts, Wheat Sprout Powder 25 parts, modified dietary fiber 12 parts, papaya powder 9 parts, 9 parts of Lactobacillus plantarum powder, ferment powder 8 parts, Fructus Citri Limoniae powder 7 parts;

Further, described grape fruit powder is to prepare through the instantaneous enzyme denaturing of microwave high-temperature, fixation and the extract at low temperature technology such as ultrasonic waves for cleaning, sericin peptide taken cryoprotection, high-pressure pulse electric extraction, ultrasonic assistant microwave extraction, biological enzymolysis, remains the natural colored of Fructus Vitis viniferae, mouthfeel and bioactive ingredients thereof to greatest extent;

Preferably, the preparation method of described grape fruit powder, comprise the following steps, Fructus Vitis viniferae is put in the ultrasonic washing unit filling 0.1-0.3% sodium bicarbonate solution, 200-400W under room temperature, 35-45KHz cleans 5-10min, drain, each uva is arbitrarily cut into four parts, single flat is layered in microwave dryer in power 3-5kW, temperature 120-130 DEG C, dry 3-5s, then 8-10min in the sericin peptide taken solution that mass percent concentration is 8-12% it is immersed in, take out, pulverize immediately after-18--22 DEG C of freezing 30-50min, ground product particle diameter 0.3-0.5mm, it is subsequently added into the water of ground product quality 1-3 times, it is 3.5-5.5 with breast acid for adjusting pH value, at electric field intensity 25-35kV/cm under room temperature, burst length 300-500 μ s, high voltage pulse electric field processing 20-30min is carried out when pulse frequency 200-300Hz,Then carry out microwave irradiation and extraction 15-20min when power 150-300W in room temperature, simultaneously at power 200-300W, when frequency 30-40KHz, carry out ultrasonic assistant extraction; Adding the mixed enzyme of extracting solution quality 1.5-2.5%, in 40-50 DEG C of enzymolysis 30-50min, enzymolysis solution filters, and filtrate reduced in volume, lyophilization to moisture are that namely 5-8% obtains grape fruit powder;

Described mixed enzyme is cellulase, protease, pectase, tannase 2-4:1-3:1-3:1-2 Homogeneous phase mixing in mass ratio.

Further, described Wheat Sprout Powder is with Semen Tritici aestivi for primary raw material, prepares through techniques such as ultrasonic cleaning, the instantaneous process of microwave, the immersion of ultrasonic wave added biological enzymolysis, germination, dry, low-temperature grinding successively;

Preferably, the preparation method of described Wheat Sprout Powder, comprise the steps: to be placed on by Semen Tritici aestivi in the ultrasonic washing unit equipped with 0.01-0.03% sodium bicarbonate solution in power 200-400W, frequency 20-30KHz room temperature ultrasonic cleaning 3-5min, take out, drain, in frequency 2450MHz, power 3000W, temperature 38-42 DEG C, thickness of feed layer 2-4cm microwave drying 10-15s, then put temperature and be 33-36 DEG C, pH value be 6-8 soak in soak 4-6h, soak contains the compound enzyme that mass percent concentration is 0.15-0.25%, ventilate once every 15-20min, ventilation pressure 0.13-0.15MPa, simultaneously in electric field intensity 10-15kV/cm, burst length 100-300 μ s, pulse frequency 60-80Hz condition carries out high voltage pulse electric field processing, until wheat water content content is 40-45%, Semen Tritici aestivi after immersion drains, and carries out dark germination in growing floor, and dark germination temperature keeps 20-24 DEG C, and the dark germination time is 20-24h, and wheat drying to the moisture after germinateing is 5-8%, and namely low-temperature grinding, excessively 60-80 mesh sieve obtain Wheat Sprout Powder,

Described compound enzyme is cellulase, 1,4 beta-glucanase, xylanase 2-4:2-4:1-3 Homogeneous phase mixing in mass ratio.

Further, described modified dietary fiber is that through physics, chemical or biological method having of processing and obtain, the abundant soluble fiber cellulose content of strong retentiveness, dilatancy, thickening property, adsorptivity and network is high, biological activity is strong, human body beneficial flora has cellulose important, positive role by dietary fiber, compared with full diet fiber, its biological action is more powerful, also can be greatly prolonged the shelf-life of grape fruit powder;

Preferably, described modified fibre is to be obtained through enzyme enzymolysis by one or more in inulin, apple fiber, Herba avenae fatuae fiber, Semen Tritici aestivi fiber;

More preferably, the preparation method of described modified dietary fiber, comprise the following steps: by inulin, Semen Tritici aestivi fiber, Herba avenae fatuae fiber 5-7:2-4:1-3 Homogeneous phase mixing in mass ratio, add its water of quality 3-7 times, room temperature 100-300W, 35-40KHz condition supersound extraction 10-15min, then at electric field intensity 20-40kV/cm, burst length 300-500 μ s, carry out high voltage pulse electric field processing 10-15min when pulse frequency 200-400Hz; It is 4.5-6.5 with breast acid for adjusting pH value, adds the enzyme of mixture quality 0.1-0.3%, in 45-55 DEG C of enzymolysis 20-48min; Enzymolysis solution filters, and filtrate reduced in volume, lyophilization to moisture are that namely 5-8% obtains modified dietary fiber;

Described enzyme is cellulase, xylanase, laccase, pectase 1-3:1-3:1-2:1-2 Homogeneous phase mixing in mass ratio.

Further, described Lactobacillus plantarum powder is prepared with Lactobacillus plantarum CGMCCNO.11763 according to a conventional method for starting strain, and its viable bacteria content is: 7 × 10¹²-9×10¹²Cfu/g; The cryoprotective agent that wherein freeze drying process adopts is with the animal or plant containing antifreeze protein for raw material, prepared through high-pressure pulse electric extraction, ultrasonic assistant microwave extraction and compound enzyme enzymolysis, Lactobacillus plantarum powder can be effectively improved at freezing dry process Viable detection;

Preferably, described protectant preparation method, comprise the steps: winter rye, Caulis et Folium Ammopiptanthi Mongolici, sharkskin collagen protein is respectively washed, drain, 8-10:3-5:2-4 Homogeneous phase mixing in mass ratio, add the lactic acid moistening 3-8h that pH value is 3.8-4.5 of mixed material quality 0.1-1 times, pulverize immediately after-18--22 DEG C of freezing 1-2h, freezing thickness of feed layer 3-5cm, ground product particle diameter 0.5-3mm, it is subsequently added into the water of ground product quality 10-20 times, it is 3.5-5.5 with breast acid for adjusting pH value, at electric field intensity 25-35kV/cm under room temperature, burst length 300-500 μ s, high voltage pulse electric field processing 20-30min is carried out when pulse frequency 200-300Hz, then carry out microwave irradiation and extraction 15-20min when power 150-300W in room temperature, simultaneously at power 200-300W, when frequency 30-40KHz, carry out ultrasonic assistant extraction, add the compound enzyme of extracting solution quality 1-2%, in 45-55 DEG C of enzymolysis 30-50min, enzymolysis solution filters, filtrate concentrates, low-temperature grinding to particle diameter is that namely 0.1-0.3mm obtains protective agent,

Described compound enzyme is cellulase, protease, amylase, pectase, tannase 2-4:1-3:1-3:1-2:1-2 Homogeneous phase mixing in mass ratio;

Extract it is highly preferred that described microwave irradiation and extraction is batch (-type), i.e. microwave exposure 10s, interval 20s.

Further, described ferment powder preparation method is as follows: adopting the fermentation liquid that pickle fermentation produces discharge in latter stage is raw material, being filtered to remove the big grain materials such as the dish leaf in fermentation liquid and obtain fermented liquid, fermented liquid adopts the drying meanss such as lyophilization to prepare ferment powder. Cryodesiccated condition is :-30--40 degree pre-freeze 6 hours; Then lyophilizing is until water content is less than 5%. Pickle production method is with reference to Chinese patent 201110421967.3 or 201210310308.7 preparation.

The preparation method that another object of the present invention is to provide above-mentioned Fructus Vitis viniferae composite powder, comprise the steps: according to formula proportion, weigh each raw material, successively ferment powder, Lactobacillus plantarum powder, grape fruit powder, Fructus Citri Limoniae powder, Wheat Sprout Powder, papaya powder are added V-type blending tank Homogeneous phase mixing 25-35min, speed of agitator 20-40r/min, being subsequently adding modified dietary fiber uniformly mixed 12-18min, speed of agitator 40-60r/min, namely sterile filling, sealing, packaging obtain Fructus Vitis viniferae composite powder;

Preferably, described sterile filling adopts automatic powdery filling machine fill, adopts simultaneously⁶⁰Co irradiation sterilization, irradiation dose 4kGy;

Preferably, described Fructus Vitis viniferae composite powder adopts Aluminum-plastic composite bag fill; Ultraviolet or ozonization 20-40min is adopted before described Aluminum-plastic composite bag fill.

The Fructus Vitis viniferae composite powder Lactobacillus plantarum viable bacteria content prepared through said method is 6.84 × 10¹¹-9.49×10¹¹cfu/g。

Lactobacillus plantarum of the present invention (Lactobacillusplantarum) XH is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (being called for short CGMCC) on November 30th, 2015, preserving number is CGMCCNO.11763, preservation address is: North Star West Road 1, Chaoyang District, city of BeiJing, China institute 3, Institute of Microorganism, Academia Sinica, postcode: 100101.

Lactobacillus plantarum probiotic properties is as follows:

Lactobacillus plantarum CGMCCNO.11763 provided by the present invention is found to survive when pH is 1.50 through experiment, still in existing state after 1% cholate is cultivated 4 hours; Lactobacillus plantarum CGMCCNO.11763 degrading nitrite speed is fast, and capacity of decomposition reaches 10.9mg/h/kg, and this strain is when producing Pickles, and whole sweat nitrite concentration is at below 4.8mg/kg; Degrading rate of cholesterol, after fermentation 60h hour, can be reached 64.76% by CGMCCNO.11763. CGMCCNO.11763 Adhering capacity measure from coagulation rate be 95.71%.

Lactobacillus plantarum CGMCCNO.11763 is to cholesterol degradation capability study and mensuration:

Take 1mlCGMCCNO.11763 mother solution and be inoculated in MRS cholesterol fluid medium (the cholesterol level 0.1mg/ml of 10mL, pH6.2) in, the constant temperature of 37 DEG C stands and cultivates 20h respectively, 40h, 60h is standby, with the MRS cholesterol culture medium that accesses 1mL sterilized water for comparison, take bacteria liquid sample and the comparison each 1ml of liquid of above cultivation different time, 9000r/min, centrifugal 10min at 4 DEG C, obtain fermented supernatant fluid, in o-phthalaldehyde method mensuration supernatant, cholesterol level is (particularly as follows: take each supernatant 0.1ml in corresponding test tube, add glacial acetic acid 0.3ml, the o-phthalaldehyde(OPA) 0.15ml of 1mg/ml, it is slowly added into concentrated sulphuric acid 1.0ml, mix homogeneously. room temperature stands 10min, surveys light absorption value under 550nm). each process 3 repetition, in kind makes cholesterol standard curve, calculates cholesterol level and degradation rate in supernatant, and result is in Table 1. it can be seen that cholesterol is had good Degradation by CGMCCNO.11763, after fermentation 60h hour, degradation rate can reach 64.76%.

The table 1 degraded situation to cholesterol.

Degradation time (h)	0	20h	40h	60h
					Cholesterol level (mg/ml)	0.2273±0.0058	0.1356±0.0018	0.1011±0.0094	0.801±0.0231
Degrading rate of cholesterol %		40.34%	55.52%	64.76%

The bile tolerance test of Lactobacillus plantarum CGMCCNO.11763 bacterial strain:

Take CGMCCNO.11763 bacterium solution 1mL and inoculate strain in the 10mLMRS fluid medium (PH=6.4) containing different cholate (concentration gradients is 0.0%, 0.2%, 0.4%, 0.6%, 0.8%, 1%), be placed at 37 DEG C to cultivate respectively 0,2,4h, each process 3 repetition. Respectively take 1ml sample bacterium solution to mix in 9ml normal saline, prepare dilution factor solution, take 0.1ml diluent to be coated with in MRS, be inverted in 37 DEG C of biochemical cultivation cases and cultivate 48 hours (each dilution factor do 3 parallel) record and calculate the several number of bacterium on flat board. Result is in Table 2. The increment of bacterium is still after gallbladder salinity is 1% process 4h for this bacterium known

Reach 0.59 ± 0.92 × 10⁷(cfu/ml), there is good bile tolerance ability.

Table 2 bile tolerance ability detection [(± s) × 10⁷cfu/ml]

The acid resistance test of Lactobacillus plantarum CGMCCNO.11763 bacterial strain

Take HLX37 mother solution and inoculate strain in the 10mLMRS fluid medium of different pH value (pH gradient is 1.5,2.0,2.5,3.0,3.5,4.0) by 1ml, be placed at 37 DEG C to cultivate respectively 0,2,4h, each process 3 repetition. Respectively take 1ml sample bacterium solution to mix in 9ml normal saline, prepare dilute solution, take 0.1ml diluent and be coated with in MRS, in 37 DEG C of biochemical cultivation cases, be inverted the bacterium colony number cultivated on 48 hours (each dilution factor do 3 parallel) record flat board. Result is in Table 3. Illustrate that this bacterium has very strong acid-fast ability.

Table 3 acid-fast ability detection [(± s) × 10⁷cfu/ml]

The Adhering capacity of Lactobacillus plantarum CGMCCNO.11763 measures

Cultivate CGMCCNO.11763 (MRS fluid medium), bacillus coli DH 5 alpha (LB fluid medium) 24h obtains fermentation liquid, be respectively placed in 3000r/min, centrifugal 10min at 4 DEG C, collect bacterium mud, wash bacterium mud 2 times with the sterile phosphate buffer (PBS) of pH=7.0 respectively and (in bacterium colony, namely add PBS, after concussion mix homogeneously, be placed in 3000r/min, centrifugal 10min at 4 DEG C, collect thalline).From coagulation rate (%): with aseptic PBS, bacterium mud CGMCCNO.11763 to be formed in the suspension bacteria liquid that light absorption value is 0.4 ± 0.1 (A0) and the bacteria suspension at wavelength 600nm place, measure light absorption value A24 after standing 24h, be (A0 A24)/A0 from coagulation rate (%) formula. ; His coagulation rate (%): the outstanding bacterium solution of CGMCCNO.11763 and bacillus coli DH 5 alpha is adjusted to the mix suspending bacterium solution that the light absorption value at wavelength 600nm place is 0.6 ± 0.1 (A0). Measuring light absorption value A24 after standing 24H, his coagulation rate (%) formula is (A0 A24)/A0. Measurement result is in Table 5, it is known that CGMCCNO.11763 is 95.71% from coagulation rate, has very strong Adhering capacity.

Table 4 Adhering capacity table

The bacterial strain physiological property of Lactobacillus plantarum CGMCCNO.11763

Described Lactobacillus plantarum (Lactobacillusplantarum) XH is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (being called for short CGMCC) on November 30th, 2015, preserving number is CGMCCNO.11763, preservation address is: North Star West Road 1, Chaoyang District, city of BeiJing, China institute 3, Institute of Microorganism, Academia Sinica, postcode: 100101.

This bacterial strain feature is as follows: examine under a microscope, and this bacterial strain is rod-short, and Gram’s staining is positive, atrichia, does not produce spore; On solid medium, this bacterium bacterium colony is white, and smooth surface is fine and close, and form is circular, and edge is more neat.

Physicochemical characteristics is: catalase (-), gelatin liquefaction (-), indole experiment (+), mobility (-), fermentation gas (-), nitrate reductase (-), fermentation gas (-), product hydrogen sulfide gas (-), pH4.0MRS culture medium grows (+). It is accredited as Lactobacillus plantarum (Lactobacillusplantarum), called after Lactobacillus plantarum (Lactobacillusplantarum) XH through Physiology and biochemistry.

Bacterial strain can at 57 DEG C well-grown, glucose tolerance is 275g/L.

Lactobacillus plantarum of the present invention, by gathering people Li Jianshu, separates in Yoghourt from Xinjiang Uygur fellow-villager family and obtains, acquisition time on June 2nd, 2015.

Lactobacillus plantarum CGMCCNO.11763 of the present invention is found to survive when pH is 1.50 through experiment, still in existing state after 1% cholate is cultivated 4 hours; Lactobacillus plantarum CGMCCNO.11763 degrading nitrite speed is fast, and capacity of decomposition reaches 10.9mg/h/kg, and this strain is when producing Pickles, and whole sweat nitrite concentration is at below 4.8mg/kg; Degrading rate of cholesterol, after fermentation 60h hour, can be reached 64.76% by CGMCCNO.11763. CGMCCNO.11763 Adhering capacity measure from coagulation rate be 95.71%, bacterial strain can at 57 DEG C well-grown, glucose tolerance is 275g/L.

Beneficial effect:

Fructus Vitis viniferae composite powder of the present invention will have natural colored, the grape fruit powder of local flavor and mouthfeel and Lactobacillus plantarum powder, Wheat Sprout Powder, modified dietary fiber, ferment powder, the functional product science such as papaya powder are composite, not only it is effectively increased the trophism of Fructus Vitis viniferae composite powder, foodsafety and health care, and significantly improve the probiotic of Fructus Vitis viniferae composite powder and quality stability, compared with existing Fructus Vitis viniferae composite powder: its component is simple, color pool, local flavor and mouthfeel are natural, it is of high nutritive value, health care is strong, bioactive substance content is high, probiotics viable bacteria quantity is high, probiotic by force, probiotic bacteria functional metabolic species is complete, content is high, foodsafety is high, long shelf-life.Concrete test effect is shown in embodiment seven-ten two, and concrete component effect is as follows:

1. the Lactobacillus plantarum CGMCCNO.11763 of the present invention is found to survive when pH is 1.50 through experiment, still in existing state after 1% cholate is cultivated 4 hours; Lactobacillus plantarum CGMCCNO.11763 degrading nitrite speed is fast, and capacity of decomposition reaches 10.9mg/h/kg, and this strain is when producing Pickles, and whole sweat nitrite concentration is at below 4.8mg/kg; Degrading rate of cholesterol, after fermentation 60h hour, can be reached 64.76% by CGMCCNO.11763. CGMCCNO.11763 Adhering capacity measure from coagulation rate be 95.71%.

2. the cryoprotective agent that the present invention adopts when preparing Lactobacillus plantarum powder is by composite to the winter rye higher containing antifreeze protein, Caulis et Folium Ammopiptanthi Mongolici and sharkskin collagen protein science; prepare through high-pressure pulse electric extraction, ultrasonic assistant microwave extraction and biological enzymolysis; omnidistance extract at low temperature; antifreeze protein extraction ratio is high; loss is few; the protective agent antifreeze peptide content obtained is high, kind is complete, functional by force, cryoprotective effects is good, improves the viable bacteria content in Lactobacillus plantarum powder.

3. the grape fruit powder that prepared by the present invention adopts ultrasonic cleaning that Fructus Vitis viniferae carries out parasite killing to go out ovum, killing microorganisms, removal pesticide residues and heavy metal ion etc., substantially increase the foodsafety of grape fruit powder; Adopt microwave high-temperature instantaneous dry, being mainly enzyme denaturing, fixation while making Fructus Vitis viniferae section explosion puffing drying, remain the natural colored of Fructus Vitis viniferae to greatest extent, the Fructus Vitis viniferae natural colored after microwave color fixing is highly stable in the follow-up course of processing, will not variable color, fade, color and luster is very bright-coloured; Aqueous solution containing sericin peptide taken soaks rehydration, the loss of Fructus Vitis viniferae active substance that is chilled and that cause can be reduced to greatest extent, improve the extraction ratio of Fructus Vitis viniferae effective ingredient, also lay a good foundation at storage freezing-resistance for Fructus Vitis viniferae composite powder simultaneously; The extract at low temperature technology such as high-pressure pulse electric, microwave, ultrasonic, biological enzymolysis are organically combined, bioactive substance and the flavor component of Fructus Vitis viniferae can be retained to greatest extent.

4. the modified dietary fiber that prepared by the present invention is that dietary fiber is processed through physics, chemical or biological method and obtained, soluble fiber cellulose content is high, biological activity is strong, beneficial bacteria of intestinal tract group has cellulose important, positive role, compared with full diet fiber, its biological action is more powerful: 1) soluble fiber cellulose content is high, it is easier to be utilized by probiotic bacteria, probiotic bacteria can be improved in the growth of intestinal and fertility, increase kind and the quantity of probiotic bacteria flora, reduce large intestine pH value, improve intestine microenvironment; 2) powerful absorbability, after modified, cellulosic specific surface area increases, and network is enriched, and absorption affinity strengthens, and the organic molecule ability of chelating, absorption cholesterol and bile acids is higher, suppress the human body absorption to them; 3) ion-exchange capacity strengthens, and to metallic element, particularly heavy metal element adsorption effect is higher; 4) retentiveness, dilatancy, thickening property be higher and acid and alkali, alkali, salt impact, 5) resident time of adjustment and maintenance intestinal microbial population, strengthen digestion and the absorbability of intestinal, improve body immunity, finally significantly improve digestion and the absorbance of Fructus Vitis viniferae composite powder effective ingredient; 6) promote gastrointestinal peristalsis, slow down and eliminate the untoward reaction such as flatulence, abdominal distention; 7) embedding effect is strong, can effectively prevent external environment (oxygen, temperature, illumination, the humidity etc.) factor impact on product quality, stabilize the biological activity of product, extend the shelf-life of product.

5. the Semen Tritici aestivi of immersion process is carried out ultrasonic cleaning by the preparation method of Wheat Sprout Powder of the present invention, the instantaneous process of microwave and high voltage pulse electric field processing effectively prevent soak pollution microbes, avoid generation stink and penetrate in Wheat Sprout Powder product, simultaneously, can effectively strengthen the relative permeability of corn seed coat cell wall and cell membrane, improve the water absorption rate of full cereal seed, promote that seed is sprouted in advance, improve generation speed and adenosine triphosphate (ATP) content of ultra-oxygen anion free radical, the activation of the multiple enzyme of stratification early stage wheat seed and release, endosperm dissolves and functional nutrient composition, bioactive ingredients, the synthesis of antioxidant content, promote the respiratory metabolism of seed, accelerate nutrition and functional materials, bioactive ingredients, the enrichment process of antioxidant content, shorten enrichment time, improve nutrition and functional materials, bioactive ingredients, the content of antioxidant content, organically combine with biological enzymolysis, can degrade further seed coat cellulose and hemicellulose structure, increase seed coat permeability, activate various bioactive substance (endogenous enzymes etc.) vigor the strongest, enriching quantity is bigger, soluble fiber cellulose content increases therewith, trophic factors is provided for the Lactobacillus plantarum in Fructus Vitis viniferae composite powder, effect is more preferably notable, ultrasonic, microwave, high-voltage pulse electric field technology and biological enzymolysis are organically combined, shortens soak time, improve germination percentage and germination uniformity, Wheat Sprout Powder heat-sensitive substance content can be kept to greatest extent, especially antioxidant (glutathion, inositol hexaphosphate, vitamin C, polyphenol etc.), ensure the natural colored of Wheat Sprout Powder, taste and flavor to greatest extent, may also function as bactericidal action simultaneously, particularly after the instantaneous appropriateness of microwave kills embryo, in the bioactive situation not affecting Fructus Tritici aestivi seed, absorption along with moisture, wheat embryo be suppressed but do not affect the growth of every enzyme and bioactive substance and the dissolving of endosperm, blastogenesis length is very short, Repiration is weak, sprout damage is low, improve yield and the quality of Wheat Sprout Powder, significantly improve in Wheat Sprout Powder product functional, trophism, the content of bioactive substance, the health care that improve Wheat Sprout Powder (improves body immunity, remove oxygen-derived free radicals in human body, blood fat reducing, slow down aging), nutritive value and foodsafety.

6. the ferment powder that prepared by the present invention does not contain only the purebred probiotic bacterias such as acetobacter, bacillus bifidus, yeast and lactobacillus rhamnosus, and containing more, more useful in pickle fermentation process participate in and produce multiple wild microbial strains, its biological activity and probiotic more comprehensively and powerful, composite with Lactobacillus plantarum powder science prepared by the present invention, its effect is more significantly.

7. the preparation method technique of Fructus Vitis viniferae composite powder of the present invention is simple, easy to operate, low for equipment requirements; can industrialization and large-scale production; modified dietary fiber is finally mixed, has played its powerful embedding effect so that the character of product is more stable, the shelf-life is longer.

What it should be noted that Fructus Vitis viniferae composite powder of the present invention has the technical effect that the result that each component technical characteristic and method feature are mutually worked in coordination with, interacted, the not superposition of simple technical characteristic (component function), the combination of each component technical characteristic and the collaborative effect produced, considerably beyond the superposition of each monotechnics feature functionality and effect, have advanced preferably and practicality.

Detailed description of the invention

The present invention is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention is method known in those skilled in the art. It addition, embodiment is interpreted as illustrative, and unrestricted the scope of the present invention, the spirit and scope of the invention are limited only by the claims that follow. To those skilled in the art, under the premise without departing substantially from spirit and scope of the present invention, the various changes or the changes that carry out the material component in these embodiments and consumption fall within protection scope of the present invention.

Embodiment one:

Prepared by raw material

1, the preparation of Wheat Sprout Powder

Its preparation method comprises the steps:

Semen Tritici aestivi is placed in the ultrasonic washing unit equipped with 0.02% sodium bicarbonate solution in power 300W, frequency 25KHz room temperature ultrasonic cleaning 4min, take out, drain, in frequency 2450MHz, power 3000W, temperature 40 DEG C, thickness of feed layer 3cm microwave drying 13s, then putting temperature is 35 DEG C, pH value be 7 soak in soak 5h, soak contains the compound enzyme that mass percent concentration is 0.20%, ventilate once every 18min, ventilation pressure 0.14MPa, simultaneously in electric field intensity 13kV/cm, burst length 200 μ s, pulse frequency 70Hz condition carries out high voltage pulse electric field processing, until wheat water content content is 43%, Semen Tritici aestivi after immersion drains, and carries out dark germination in growing floor, and dark germination temperature keeps 22 DEG C, and the dark germination time is 22h, and wheat drying to the moisture after germinateing is 6.5%, and namely low-temperature grinding, excessively 70 mesh sieves obtain Wheat Sprout Powder,

Above-mentioned compound enzyme is cellulase, 1,4 beta-glucanase, xylanase 3:3:2 Homogeneous phase mixing in mass ratio.

2, the preparation of modified dietary fiber

Its preparation method comprises the following steps:

By inulin, Semen Tritici aestivi fiber, Herba avenae fatuae fiber 6:3:2 Homogeneous phase mixing in mass ratio, add the water of its quality 5 times, room temperature 200W, 38KHz condition supersound extraction 13min, then at electric field intensity 30kV/cm, burst length 400 μ s, carries out high voltage pulse electric field processing 13min when pulse frequency 300Hz; It is 5.5 with breast acid for adjusting pH value, adds the enzyme of mixture quality 0.2%, in 50 DEG C of enzymolysis 34min; Enzymolysis solution filters, and filtrate reduced in volume, lyophilization to moisture are 6.5% namely obtain modified dietary fiber;

Above-mentioned enzyme is cellulase, xylanase, laccase, pectase 2:2:1.5:1.5 Homogeneous phase mixing in mass ratio.

3, the preparation of Lactobacillus plantarum powder

Lactobacillus plantarum powder is prepared with Lactobacillus plantarum CGMCCNO.11763 according to a conventional method for starting strain, and its viable bacteria content is: 8 × 10¹²Cfu/g; The cryoprotective agent that wherein freeze drying process adopts is with the animal or plant containing antifreeze protein for raw material, prepared through high-pressure pulse electric extraction, ultrasonic assistant microwave extraction and compound enzyme enzymolysis, Lactobacillus plantarum powder can be effectively improved at freezing dry process Viable detection;

Above-mentioned protectant preparation method comprises the steps:

Winter rye, Caulis et Folium Ammopiptanthi Mongolici, sharkskin collagen protein are respectively washed, drain, 9:4:3 Homogeneous phase mixing in mass ratio, add the lactic acid moistening 5h that pH value is 4.1 of mixed material quality 0.5 times, pulverize immediately after-20 DEG C of freezing 1.5h, freezing thickness of feed layer 4cm, ground product particle diameter 2mm, it is subsequently added into the water of ground product quality 15 times, it is 4.5 with breast acid for adjusting pH value, at electric field intensity 30kV/cm under room temperature, burst length 400 μ s, carries out high voltage pulse electric field processing 25min when pulse frequency 250Hz;Then microwave irradiation and extraction 18min is carried out in room temperature when power 225W, its microwave exposure 10s, interval 20s, simultaneously at power 250W, when frequency 35KHz, carry out ultrasonic assistant extraction; Add the compound enzyme of extracting solution quality 1.5%, in 50 DEG C of enzymolysis 40min; Enzymolysis solution filters, filtrate concentrates, low-temperature grinding to particle diameter is that namely 0.2mm obtains protective agent;

Above-mentioned compound enzyme is cellulase, protease, amylase, pectase, tannase 3:2:2:1.5:1.5 Homogeneous phase mixing in mass ratio;

4, the preparation of ferment powder

Its preparation method is as follows:

Adopting the fermentation liquid that pickle fermentation produces discharge in latter stage is raw material, is filtered to remove the big grain materials such as the dish leaf in fermentation liquid and obtains fermented liquid, and fermented liquid adopts the drying meanss such as lyophilization to prepare ferment powder. Cryodesiccated condition is :-35 degree pre-freezes 6 hours; Then lyophilizing is until water content is 3%. Pickle production method is prepared with reference to Chinese patent 201110421967.3.

Wheat Sprout Powder that following example two to six use, modified dietary fiber, Lactobacillus plantarum powder, ferment powder are by embodiment one preparation.

Embodiment two:

The wherein preparation method of grape fruit powder, comprises the following steps:

Fructus Vitis viniferae is put in the ultrasonic washing unit filling 0.2% sodium bicarbonate solution, 300W under room temperature, 30KHz cleans 8min, drain, each uva is arbitrarily cut into four parts, single flat is layered in microwave dryer in power 4kW, temperature 125 DEG C, dry 4s, then 9min in the sericin peptide taken solution that mass percent concentration is 10% it is immersed in, take out, pulverize immediately after-20 DEG C of freezing 40min, ground product particle diameter 0.4mm, it is subsequently added into the water of ground product quality 2 times, it is 4.5 with breast acid for adjusting pH value, at electric field intensity 30kV/cm under room temperature, burst length 400 μ s, high voltage pulse electric field processing 25min is carried out when pulse frequency 250Hz, then carry out microwave irradiation and extraction 18min when power 225W in room temperature, simultaneously at power 250W, when frequency 35KHz, carry out ultrasonic assistant extraction, adding the mixed enzyme of extracting solution quality 2.0%, in 45 DEG C of enzymolysis 40min, enzymolysis solution filters, and filtrate reduced in volume, lyophilization to moisture are 6.5% namely obtain grape fruit powder,

Above-mentioned mixed enzyme is cellulase, protease, pectase, tannase 3:2:2:1.5 Homogeneous phase mixing in mass ratio.

The preparation method of Fructus Vitis viniferae composite powder, comprises the steps:

According to formula proportion, weigh each raw material, successively ferment powder, Lactobacillus plantarum powder, grape fruit powder, Fructus Citri Limoniae powder, Wheat Sprout Powder, papaya powder are added V-type blending tank Homogeneous phase mixing 30min, speed of agitator 30r/min, it is subsequently adding modified dietary fiber uniformly mixed 15min, speed of agitator 50r/min, namely sterile filling, sealing, packaging obtain Fructus Vitis viniferae composite powder;

Above-mentioned sterile filling adopts automatic powdery filling machine fill, adopts simultaneously⁶⁰Co irradiation sterilization, irradiation dose 4kGy;

Above-mentioned Fructus Vitis viniferae composite powder adopts Aluminum-plastic composite bag fill; Disinfection by ultraviolet light 30min is adopted before described Aluminum-plastic composite bag fill.

The Fructus Vitis viniferae composite powder Lactobacillus plantarum viable bacteria content prepared through said method is 9.49 × 10¹¹cfu/g。

Embodiment three:

Grape fruit powder 35 parts, Wheat Sprout Powder 23 parts, modified dietary fiber 11 parts, papaya powder 8 parts, 8 parts of Lactobacillus plantarum powder, ferment powder 7 parts, Fructus Citri Limoniae powder 6 parts;

Fructus Vitis viniferae is put in the ultrasonic washing unit filling 0.1% sodium bicarbonate solution, 200W under room temperature, 35KHz cleans 5min, drain, each uva is arbitrarily cut into four parts, single flat is layered in microwave dryer in power 3kW, temperature 120 DEG C, dry 3s, then 8min in the sericin peptide taken solution that mass percent concentration is 8% it is immersed in, take out, pulverize immediately after-18 DEG C of freezing 30min, ground product particle diameter 0.3mm, it is subsequently added into the water of ground product quality 1 times, it is 3.5 with breast acid for adjusting pH value, at electric field intensity 25kV/cm under room temperature, burst length 300 μ s, high voltage pulse electric field processing 20min is carried out when pulse frequency 200Hz, then carry out microwave irradiation and extraction 15min when power 150W in room temperature, simultaneously at power 200W, when frequency 30KHz, carry out ultrasonic assistant extraction, adding the mixed enzyme of extracting solution quality 1.5%, in 40 DEG C of enzymolysis 30min, enzymolysis solution filters, and filtrate reduced in volume, lyophilization to moisture are 5% namely obtain grape fruit powder,

Above-mentioned mixed enzyme is cellulase, protease, pectase, tannase 2:1:1:1 Homogeneous phase mixing in mass ratio.

According to formula proportion, weigh each raw material, successively ferment powder, Lactobacillus plantarum powder, grape fruit powder, Fructus Citri Limoniae powder, Wheat Sprout Powder, papaya powder are added V-type blending tank Homogeneous phase mixing 25min, speed of agitator 20r/min, it is subsequently adding modified dietary fiber uniformly mixed 12min, speed of agitator 40r/min, namely sterile filling, sealing, packaging obtain Fructus Vitis viniferae composite powder;

Above-mentioned Fructus Vitis viniferae composite powder adopts Aluminum-plastic composite bag fill; Disinfection by ultraviolet light 20min is adopted before described Aluminum-plastic composite bag fill.

The Fructus Vitis viniferae composite powder Lactobacillus plantarum viable bacteria content prepared through said method is 8.13 × 10¹¹cfu/g。

Embodiment four:

Grape fruit powder 45 parts, Wheat Sprout Powder 27 parts, modified dietary fiber 13 parts, papaya powder 10 parts, 10 parts of Lactobacillus plantarum powder, ferment powder 9 parts, Fructus Citri Limoniae powder 8 parts;

Fructus Vitis viniferae is put in the ultrasonic washing unit filling 0.3% sodium bicarbonate solution, 400W under room temperature, 45KHz cleans 10min, drain, each uva is arbitrarily cut into four parts, single flat is layered in microwave dryer in power 5kW, temperature 130 DEG C, dry 5s, then 10min in the sericin peptide taken solution that mass percent concentration is 12% it is immersed in, take out, pulverize immediately after-22 DEG C of freezing 50min, ground product particle diameter 0.5mm, it is subsequently added into the water of ground product quality 3 times, it is 5.5 with breast acid for adjusting pH value, at electric field intensity 35kV/cm under room temperature, burst length 500 μ s, high voltage pulse electric field processing 30min is carried out when pulse frequency 300Hz, then carry out microwave irradiation and extraction 20min when power 300W in room temperature, simultaneously at power 300W, when frequency 40KHz, carry out ultrasonic assistant extraction, adding the mixed enzyme of extracting solution quality 2.5%, in 50 DEG C of enzymolysis 50min, enzymolysis solution filters, and filtrate reduced in volume, lyophilization to moisture are 8% namely obtain grape fruit powder,

Above-mentioned mixed enzyme is cellulase, protease, pectase, tannase 4:3:3:2 Homogeneous phase mixing in mass ratio.

According to formula proportion, weigh each raw material, successively ferment powder, Lactobacillus plantarum powder, grape fruit powder, Fructus Citri Limoniae powder, Wheat Sprout Powder, papaya powder are added V-type blending tank Homogeneous phase mixing 35min, speed of agitator 40r/min, it is subsequently adding modified dietary fiber uniformly mixed 18min, speed of agitator 60r/min, namely sterile filling, sealing, packaging obtain Fructus Vitis viniferae composite powder;

Above-mentioned Fructus Vitis viniferae composite powder adopts Aluminum-plastic composite bag fill; Disinfection by ultraviolet light 40min is adopted before described Aluminum-plastic composite bag fill.

The Fructus Vitis viniferae composite powder Lactobacillus plantarum viable bacteria content prepared through said method is 6.84 × 10¹¹cfu/g。

Embodiment five:

Grape fruit powder 30 parts, Wheat Sprout Powder 20 parts, modified dietary fiber 8 parts, papaya powder 6 parts, 6 parts of Lactobacillus plantarum powder, ferment powder 5 parts, Fructus Citri Limoniae powder 5 parts;

Fructus Vitis viniferae is put in the ultrasonic washing unit filling 0.1% sodium bicarbonate solution, 400W under room temperature, 35KHz cleans 10min, drain, each uva is arbitrarily cut into four parts, single flat is layered in microwave dryer in power 3kW, temperature 130 DEG C, dry 3s, then 8min in the sericin peptide taken solution that mass percent concentration is 12% it is immersed in, take out, pulverize immediately after-22 DEG C of freezing 30min, ground product particle diameter 0.5mm, it is subsequently added into the water of ground product quality 1 times, it is 5.5 with breast acid for adjusting pH value, at electric field intensity 25kV/cm under room temperature, burst length 500 μ s, high voltage pulse electric field processing 30min is carried out when pulse frequency 200Hz, then carry out microwave irradiation and extraction 20min when power 150W in room temperature, simultaneously at power 200W, when frequency 40KHz, carry out ultrasonic assistant extraction, adding the mixed enzyme of extracting solution quality 1.5%, in 50 DEG C of enzymolysis 30min, enzymolysis solution filters, and filtrate reduced in volume, lyophilization to moisture are 8% namely obtain grape fruit powder,

Above-mentioned mixed enzyme is cellulase, protease, pectase, tannase 2:3:1:2 Homogeneous phase mixing in mass ratio.

According to formula proportion, weigh each raw material, successively ferment powder, Lactobacillus plantarum powder, grape fruit powder, Fructus Citri Limoniae powder, Wheat Sprout Powder, papaya powder are added V-type blending tank Homogeneous phase mixing 28min, speed of agitator 25r/min, it is subsequently adding modified dietary fiber uniformly mixed 13min, speed of agitator 45r/min, namely sterile filling, sealing, packaging obtain Fructus Vitis viniferae composite powder;

Above-mentioned Fructus Vitis viniferae composite powder adopts Aluminum-plastic composite bag fill; Ozonization 20min is adopted before described Aluminum-plastic composite bag fill.

The Fructus Vitis viniferae composite powder Lactobacillus plantarum viable bacteria content prepared through said method is 7.26 × 10¹¹cfu/g。

Embodiment six:

Grape fruit powder 50 parts, Wheat Sprout Powder 30 parts, modified dietary fiber 15 parts, papaya powder 12 parts, 12 parts of Lactobacillus plantarum powder, ferment powder 10 parts, Fructus Citri Limoniae powder 10 parts;

Fructus Vitis viniferae is put in the ultrasonic washing unit filling 0.3% sodium bicarbonate solution, 200W under room temperature, 45KHz cleans 5min, drain, each uva is arbitrarily cut into four parts, single flat is layered in microwave dryer in power 5kW, temperature 120 DEG C, dry 5s, then 10min in the sericin peptide taken solution that mass percent concentration is 8% it is immersed in, take out, pulverize immediately after-18 DEG C of freezing 50min, ground product particle diameter 0.3mm, it is subsequently added into the water of ground product quality 3 times, it is 3.5 with breast acid for adjusting pH value, at electric field intensity 35kV/cm under room temperature, burst length 300 μ s, high voltage pulse electric field processing 20min is carried out when pulse frequency 300Hz,Then carry out microwave irradiation and extraction 15min when power 300W in room temperature, simultaneously at power 300W, when frequency 30KHz, carry out ultrasonic assistant extraction; Adding the mixed enzyme of extracting solution quality 2.5%, in 40 DEG C of enzymolysis 50min, enzymolysis solution filters, and filtrate reduced in volume, lyophilization to moisture are 5% namely obtain grape fruit powder;

Above-mentioned mixed enzyme is cellulase, protease, pectase, tannase 4:1:3:1 Homogeneous phase mixing in mass ratio.

According to formula proportion, weigh each raw material, successively ferment powder, Lactobacillus plantarum powder, grape fruit powder, Fructus Citri Limoniae powder, Wheat Sprout Powder, papaya powder are added V-type blending tank Homogeneous phase mixing 33min, speed of agitator 35r/min, it is subsequently adding modified dietary fiber uniformly mixed 16min, speed of agitator 55r/min, namely sterile filling, sealing, packaging obtain Fructus Vitis viniferae composite powder;

Above-mentioned Fructus Vitis viniferae composite powder adopts Aluminum-plastic composite bag fill; Ozonization 40min is adopted before described Aluminum-plastic composite bag fill.

The Fructus Vitis viniferae composite powder Lactobacillus plantarum viable bacteria content prepared through said method is 6.99 × 10¹¹cfu/g。

The change of T-CHOL after experimental example seven table grapes composite powder

Selecting the adult 48 of T-CHOL 180mg/dl-250mg/dl, men and women half and half, is randomly divided into three groups; 150 milliliters of mineral waters are drunk in first group of dinner every day; Second group of common commercial glucose composite powder 150g of dinner every day Instant Drinks, the Fructus Vitis viniferae composite powder 150g of the 3rd group of dinner every day Instant Drinks embodiment of the present invention 2, every day period eats same food, and food includes meat, egg, vegetable and fruit. Respectively at the blood of the previous day that experiment starts and the 20th, 40,60 days acquisition test persons, measuring the total cholesterol level in blood, result is table 1 such as:

Table 1: total cholesterol level testing result in blood

Time	0 day	20 days	40 days	60 days
					First group (mg/dl)	202.3	203.8	206.2	209.3
Second group (mg/dl)	207.6	202.5	200.8	198.6
					3rd group (mg/dl)	208.8	200.9	189.5	167.3

As seen from the above table after the Fructus Vitis viniferae composite powder of the Instant Drinks embodiment of the present invention 2, the content generation significant change of the T-CHOL in adult's blood. Compared with common commercial glucose composite powder, the content of the T-CHOL in adult's blood is significantly reduced by Fructus Vitis viniferae composite powder of the present invention, and the content of the T-CHOL in mineral water composition year human blood significantly increases, although commercial glucose composite powder decreases, but compared with product of the present invention, effect is notable, it follows that the present invention adopts Fructus Vitis viniferae composite powder prepared by the specific bacterial strain with reduction cholesterol characteristic to have the health-care effect well reducing cholesterol.

It should be understood that the Fructus Vitis viniferae composite powder prepared by embodiment of the present invention 3-6 has above-mentioned technique effect equally, between each embodiment, diversity is not notable.

Embodiment eight Fructus Vitis viniferae composite powder shelf-life implants living preparation of lactobacillus stable content test of the present invention

Take the Fructus Vitis viniferae composite powder of the embodiment of the present invention 2 preparation in room temperature 22-25 DEG C, store 3 months, 6 months, 12 months, 24 months, 36 months respectively and measure Lactobacillus plantarum viable bacteria content under ventilation condition, result is table 2 such as:

Table 2: shelf-life implants living preparation of lactobacillus content detection result

Result above shows: the storage stability of Fructus Vitis viniferae composite powder of the present invention is better, environment resistant (temperature, appropriateness, oxygen, illumination, moisture etc.) influence factor's ability is big, shelf-life Lactobacillus plantarum viable bacteria content loss rate maximum (36 months) is 15%, higher than existing like product viable bacteria content, loss rate is low, long shelf-life, reflects that other bioactive ingredients of Fructus Vitis viniferae composite powder has same storage stability simultaneously.

It should be understood that embodiment of the present invention 3-6 has above-mentioned experiment effect equally, between each embodiment and little with above-mentioned experiment effect diversity.

The sensory evaluation test of embodiment nine Fructus Vitis viniferae composite powder of the present invention

The Fructus Vitis viniferae composite powder that the embodiment of the present invention 2 is prepared by 24 personnel is invited to judge with the Fructus Vitis viniferae composite powder of commercially available two kinds of similar identical dates of manufacture, sense organ is given a mark, wherein specialty and each 12 of layman, professional is young, middle aged, each 4 of old age, men and women half and half, layman is juvenile, young, middle aged, each 3 of old age, and men and women half and half; Marking includes outward appearance (20 points), quality (25 points), local flavor (30 points), four aspects of mouthfeel (25 points), and marking personnel independently carry out, and are independent of each other, and judges result with guarantee accurate. Having added up judging result, equal score value takes approximation, retains integer, is specifically shown in table 3:

Table 3: sensory evaluation statistical result

Note: show significant difference (P < 0.05) with the different lowercase alphabet of mark in a line, the different capitalization of mark represents difference extremely notable (P < 0.01), indicates same letter and represents difference not notable (P > 0.05).

Result above shows, any one will be substantially better than commercial glucose composite powder to Fructus Vitis viniferae composite powder prepared by the present invention from outward appearance, quality, local flavor and mouthfeel, particularly outward appearance, local flavor and mouthfeel are fabulous, also are adapted for different age group, the consumer of different hierarchy of consumption eats simultaneously.

It should be understood that Fructus Vitis viniferae composite powder prepared by embodiment of the present invention 3-6 has above-mentioned experiment effect equally, between each embodiment and little with above-mentioned experiment effect diversity.

The embodiment ten Fructus Vitis viniferae of the present invention probiotic test of composite powder

The Fructus Vitis viniferae composite powder embodiment of the present invention 2 prepared, preparing into viable count with sterilized water is 2 × 10¹⁰The Lactobacillus plantarum solution of CFU/mL, saves backup in 4 DEG C;

1, the 10mL Lactobacillus plantarum solution kept of going bail for is injected in test tube 1, adopts ten times of stepwise dilutions to 10^-8, take 1mL diluent on flat board, the MRS agar culture medium being cooled to 45 DEG C poured on flat board (sterilizing), shake up rapidly after sterilizing. Will be equipped with the test tube 2 of 10mL Lactobacillus plantarum solution again to be placed in 80-90 DEG C of water-bath and heat 15-25min, take the Lactobacillus plantarum solution after heating and carry out ten times of stepwise dilutions to 10^-8, take 1mL diluent on flat board, the MRS agar culture medium being cooled to 45 DEG C poured into upper at flat board (sterilizing) and shake up rapidly after sterilizing. Finally the flat board before heating and after heating is all cultivated under 35 DEG C of conditions 24h, calculates the quantity before and after heating.

It is shown that Viable detection has reached more than 88%.

2, the resistance test of simulated gastric fluid and intestinal juice: the hydrochloric acid 16.4mL taking 100g/L adds distilled water diluting, make pH value respectively 1.5,2.5 and 3.5, take 100mL dilute hydrochloric acid solution, it is separately added into 1g pepsin, it is made fully to dissolve, obtaining simulated gastric fluid, microporous filter membrane degerming (0.22 μm) is standby. Taking potassium dihydrogen phosphate 6.8g, the 500mL that adds water makes dissolving, regulates pH value to 6.8 with 0.1moL/L sodium hydroxide solution; Separately taking trypsin 10g, the 100mL that adds water makes dissolving, after two liquid mixing, is diluted with water to 1000ml, obtains simulated intestinal fluid, and microporous filter membrane degerming (0.22 μm) is standby. Take the 1mL Lactobacillus plantarum solution kept to join in the simulated gastric fluid of 9mL (i.e. ten times of stepwise dilutions), and fully mix on the oscillator rapidly, be subsequently placed in 30-45 DEG C of quiescent culture 2-4h.Take out culture fluid respectively when 1h, 2h, 3h, 4h and count remaining viable count immediately, comparing with former viable count, it is shown that Viable detection is 98%. Then each 1mL of the culture fluid digesting different time it is taken in simulated gastric fluid, it is inoculated in the simulated intestinal fluid that 9mLpH value is 6.8 respectively, it is placed in 30-45 DEG C of quiescent culture 2-4h, and respectively 0,3,6,24h sampling, measure its viable count, comparing with former viable count, result shows that Viable detection is 99%.

3, simulating the resistance test of cholate: make the solution of 1g/L with pancreatin, and add the Fel Sus domestica salt of 0.8% in the solution, the NaOH adjustment pH with 10% is 8.0, then with 0.45 μm of micro-filtrate membrane filtration degerming. The Lactobacillus plantarum solution inoculum kept by 0.5mL is simulated in cholate to 4.5mL, obtains culture fluid, the viable count of counting remaining after cultivating 24h. By culture fluid in sterile saline ten times of stepwise dilutions to 10^-8, and pour into MRS, it is subsequently placed in 35 DEG C of quiescent culture 24h. Result shows that Viable detection is 99%.

Above result of the test shows, the Lactobacillus plantarum probiotic (thermostability, resistance to pH, bile tolerance) in Fructus Vitis viniferae composite powder of the present invention is relatively strong, is especially suitable for human intestines and stomach's environment, and in simulated gastrointestinal environments, survival rate is big, can be effectively improved gastrointestinal function.

Embodiment 11 Fructus Vitis viniferae of the present invention composite powder mouse intestinal performance test

Choosing common Kunming white mice 60, male and female half and half, 18-20g, conventional word is supported. Therefrom random choose 40,9:00 gavage lincomycin hydrochloride 0.2mL every morning (20mg)/only, other as a control group, every day same time gavage equivalent sterile saline, continuous one week, prepare the mouse model of alteration of intestinal flora. Model group mouse diet declines, and dead and phenomenon of significantly suffering from diarrhoea does not occur, arranges soft excrement, and profile normal aqueous divides more, and bedding and padding are moist. By 40 alteration of intestinal flora mices, being randomly divided into 2 groups, one group 20 is only used as treatment group, the Lactobacillus plantarum solution 0.5ml (2 × 10 that every day, gavage was kept¹⁰Cfu/ml)/only, another 20 are only used as natural recovering group, every day same time gavage equivalent sterile saline, continuous two weeks. 21 days whole experimental periods, observing growth and the defecation situation of white mice every day, weigh in the 8th, 21 days mices to Fructus Vitis viniferae composite powder treatment group and natural recovering group, calculate each group of weight average rate of increase, result is table 4 such as; Within every 5 days, surveying each group of stool in mice escherichia coli quantity, calculate average, result is table 5 such as. Take stool in mice and be about 0.1g, in aseptic operating platform, add 3 beades (adding 0.5mL diluent with 0.1g excrement sample), dilute and inoculate maconkey agar culture medium, calculate every gram of coliform count wet in just.

Table 4: mice Gain weight

Packet	Average starting weight (g/ is only)	Average end weight (g/ is only)	Average rate of increase (%)
				Natural recovering group	20.69±1.33	27.34±1.59	32.14^a
Treatment group	20.41±1.45	34.28±1.62	67.96^b

Table 5: the situation of coliform count in stool in mice

Fructus Vitis viniferae composite powder treatment group Mouse Weight average rate of increase (67.96%) is significantly higher than natural recovering group (32.14%);Feed solution rear intestinal escherichia coli quantity to be remarkably decreased, reduce by 97.07%, it is substantially less than natural recovering group (24.78%), show the rapid field planting in white mice intestinal of the Lactobacillus plantarum in Fructus Vitis viniferae composite powder of the present invention, form dominant microflora, and effectively suppress the growth and breeding of the pathogen such as escherichia coli, and resident time is long, continues, effectively improves intestinal performance.

The impact on immunity of organisms of the embodiment 12 Fructus Vitis viniferae composite powder of the present invention

1 experiment purpose

Test (mouse forced swimming) by exercise tolerance, verify the raising immunity of Fructus Vitis viniferae composite powder of the present invention, antifatigue effect.

2 experiment materials and reagent

2.1 for reagent thing:

Commercial glucose composite powder (G1); Commercial glucose composite powder (G2); Fructus Vitis viniferae composite powder (G3-G7) prepared by embodiment of the present invention 2-6.

2.2 reagent:

Liver/muscle glycogen testing cassete, builds up institute of biological products purchased from Nanjing; Concentrated sulphuric acid (AR), Nanjing Chemistry Reagent Co., Ltd.; Normal saline, Shangdong Changfu Jiejing Pharmaceutical Industry Co., Ltd..

3. laboratory animal

ICR mice, ♂, cleaning grade, body weight 18-22g, Ningxia Medical University's comparative medicine center provide, the free diet of mice during experiment.

4. key instrument

Aluminum swimming trunk (50cm × 50cm × 40cm), galvanized wire, low-temperature and high-speed centrifuge: 5804R type, Eppendrof company; Water-bath: DK-S26 type, the grand experimental facilities company limited of upper Nereid; Electronic scale: BS224S type, Sartorius company; Stopwatch, thermometer

5. experiment packet

5.1 dosage packets and given the test agent give the time and at random mice are divided into 8 groups, often group 10,1st group to the 7th group medicine giving G1～G7 respectively, 8th group is blank group, give isopyknic distilled water, the often every average daily gavage of group 1 time, gavage volume is 0.2ml/10g, gives given the test agent continuously 30 days.

5.2 sample preparations the 1st group to the 7th group: weigh 2.25g drug sample, be assigned to 150ml with distilled water; Blank group: distilled water 150ml.

6. experimental technique

After 6.1 swimming with a load attached to the body experiment last administration 30min, putting mice in swimming trunk, the depth of water is no less than 30cm, water temperature 25 ± 1 DEG C, the sheet lead of rat-tail root load 5% body weight, and record mice swimming started to the dead time, as mice swimming time.

After 6.2 mice serum carbamide measure last administration 30min, not swimming with a load attached to the body 90min in the water that temperature is 30 DEG C, eyeball blood sampling 0.5mL (being not added with anticoagulant) is plucked after rest 60min, put 4 DEG C of refrigerator 3h, after hemopexis, the centrifugal 15min of 2000r/min, takes serum and send clinical laboratory of Affiliated Hospital of Ningxia Medical University to detect.

After the mensuration last administration 30min of 6.3 hepatic glycogen, not swimming with a load attached to the body 90min in the water that temperature is 25 ± 1 DEG C, cervical dislocation puts to death mice, clean with normal saline, and with, after filter paper suck dry moisture, accurately weighing liver 100mg, hepatic glycogen detection kit detection Mouse Liver glycogen content.

Blood sampling after the mensuration last administration 30min of 6.4 blood lactase acid, does not then bear a heavy burden and stops after the water went swimming 10min that temperature is 30 DEG C. Lactic acid instrument assay method: respectively before swimming, each blood sampling 20 μ L add in 40 μ L rupture of membranes liquid after rest 20min after swimming, after swimming, fully vibration smudge cells lactic acid instrument measures immediately. (blood lactase acid area under curve=5 × (after front blood lactase acid value+3 × swimming of swimming the blood lactase acid value of 20min after blood lactase acid value+2 × swimming of 0min)

7. observation index walking weight load, blood lactase acid, carbamide, glycogen initial value

8. statistical method experimental data x ± s represents, adopts t inspection to compare between organizing

9. experimental result

The impact on Mouse Weight of the 9.1 Fructus Vitis viniferae composite powders of the present invention

Each group mice is after giving G1～G9 medicine, before, in, post-weight is shown in shown in following table respectively, and each original body mass organizing mice and weightening finish body weight compare equal no difference of science of statistics (P > 0.05) with matched group, it was shown that G1～G9 medicine is all without obvious toxicity.Experimental result refers to table 6.

The original body mass of table 6 swimming with a load attached to the body experiment mice, mid-term body weight and terminate body weight

The impact on the mice burden swimming time of the 9.2 Fructus Vitis viniferae composite powders of the present invention

After per os gives mice G1～G7 medicine, G1～G2 medicine compares with blank group, the mice burden swimming time can be obviously prolonged, there is significant difference (P < 0.05), Fructus Vitis viniferae composite powder G3～G7 medicine of the present invention compares with blank group, can significantly extend the mice burden swimming time, there is pole significant difference (P < 0.01), and be substantially better than G1～G2 medicine. The results detailed in Table 7.

The impact on the mice burden swimming time of the table 7 Fructus Vitis viniferae composite powder

" * " p < 0.05vs blank;

" * * " p < 0.01vs blank;

9.3 Fructus Vitis viniferae composite powders of the present invention are on the impact of blood lactase acid before and after mouse movement

After per os gives the Fructus Vitis viniferae composite powder of the mice present invention, blood lactase acid area under curve after mouse movement is compared by Fructus Vitis viniferae composite powder G3～G7 medicine of the present invention with matched group significant difference (P < 0.05), decrease though G1～G2 medicine group Mouse Blood lactic acid area under curve compares with matched group, but and no difference of science of statistics (P > 0.05). Result is in Table 8.

Table 8 Fructus Vitis viniferae composite powder of the present invention is on the impact of blood lactase acid level before and after mouse movement

" * " p < 0.05vs blank;

The impact on Mouse Liver glycogen of the 9.4 Fructus Vitis viniferae composite powders of the present invention

After per os gives mice G1～G7 medicine, G1～G2 medicine compares with blank group, Mouse Liver glycogen content all has obvious rising, there is significant difference (P < 0.05), Fructus Vitis viniferae composite powder G3～G7 medicine of the present invention compares with blank group, Mouse Liver glycogen content all has obvious rising, has pole significant difference (P < 0.01), and is substantially better than G1～G2 medicine. The results detailed in Table 9.

The impact on Mouse Liver glycogen content of the table 9 Fructus Vitis viniferae composite powder of the present invention

" * " p < 0.05vs blank;

" * * " p < 0.01vs blank;

The impact on mice serum carbamide of the 9.5 Fructus Vitis viniferae composite powders of the present invention

After per os gives mice G1～G7 medicine, G1～G2 medicine group compares with blank group, after mouse movement, serum urea content all has obvious reduction, there is significant difference (P < 0.05), Fructus Vitis viniferae composite powder G3～G7 medicine of the present invention compares with blank group, after mouse movement, serum urea content all has obvious reduction, has pole significant difference (P < 0.01), and is substantially better than G1～G2 medicine. The results detailed in Table 10.

The impact on mice serum urea content of the table 10 Fructus Vitis viniferae composite powder of the present invention

" * " p < 0.05vs blank;

" * * " p < 0.01vs blank;

10. experiment conclusion

This experiment is tested mainly through mice burden swimming, and the deposit simultaneously detecting Mouse Liver glycogen observes the effect of Fructus Vitis viniferae composite powder of the present invention raising immunity, resisting fatigue. Preliminary Results shows below:

1, G3～G7 Fructus Vitis viniferae composite powder of the present invention all can extend the mice burden swimming time (P < 0.01), and effect is substantially better than the Fructus Vitis viniferae composite powder of other G1～G2.

2, biochemistry detection aspect shows, the each dosage group of G3～G7 Fructus Vitis viniferae composite powder of the present invention all can reduce move after lactic acid content produced by glucose anerobic glycolysis in mice serum, compare with matched group and have significant difference (P < 0.05), and although the Fructus Vitis viniferae composite powder of other G1～G2 also can reduce after motion lactic acid content produced by glucose anerobic glycolysis in mice serum, but compare with matched group, no difference of science of statistics (P > 0.05);

3, each dosage group of G3～G7 Fructus Vitis viniferae composite powder of the present invention all can significantly improve the deposit (P < 0.01) of glycogen in mouse liver, and effect is substantially better than the Fructus Vitis viniferae composite powder of other G1～G2;

4, metabolic arthritis model finds, G3～G7 Fructus Vitis viniferae composite powder of the present invention can significantly reduce the content (P < 0.01) of urea in serum after mice is swum, and effect is substantially better than other G1～G2 Fructus Vitis viniferae composite powder;

11. conclusion

Above-mentioned experiment proves that Fructus Vitis viniferae composite powder of the present invention can significantly improve immunity of organisms, improve muscle power and the endurance of mice, the content of urea in serum and lactic acid after reduction mouse movement, and the deposit of glycogen in mouse liver can be significantly improved, contribute to alleviating the fatigue that sports load causes; The time that mice burden swimming to power exhausts can be extended.

Claims

1. a Fructus Vitis viniferae composite powder, is mainly prepared by the raw material of following parts by weight: grape fruit powder 30-50 part, Wheat Sprout Powder 20-30 part, modified dietary fiber 8-15 part, papaya powder 6-12 part, Lactobacillus plantarum powder 6-12 part, ferment powder 5-10 part, Fructus Citri Limoniae powder 5-10 part;

Described Lactobacillus plantarum powder is to prepare according to a conventional method with Lactobacillus plantarum CGMCCNO.11763 for starting strain.

2. Fructus Vitis viniferae composite powder as claimed in claim 1, it is characterised in that mainly prepared by the raw material of following parts by weight: grape fruit powder 35-45 part, Wheat Sprout Powder 23-27 part, modified dietary fiber 11-13 part, papaya powder 8-10 part, Lactobacillus plantarum powder 8-10 part, ferment powder 7-9 part, Fructus Citri Limoniae powder 6-8 part.

3. Fructus Vitis viniferae composite powder as claimed in claim 1, it is characterised in that mainly prepared by the raw material of following parts by weight: grape fruit powder 40 parts, Wheat Sprout Powder 25 parts, modified dietary fiber 12 parts, papaya powder 9 parts, 9 parts of Lactobacillus plantarum powder, ferment powder 8 parts, Fructus Citri Limoniae powder 7 parts.

4. the Fructus Vitis viniferae composite powder as described in as arbitrary in claim 1-3, it is characterized in that, the preparation method of described grape fruit powder, comprise the following steps, Fructus Vitis viniferae is put in the ultrasonic washing unit filling 0.1-0.3% sodium bicarbonate solution, 200-400W under room temperature, 35-45KHz cleans 5-10min, drain, each uva is arbitrarily cut into four parts, single flat is layered in microwave dryer in power 3-5kW, temperature 120-130 DEG C, dry 3-5s, then 8-10min in the sericin peptide taken solution that mass percent concentration is 8-12% it is immersed in, take out, pulverize immediately after-18--22 DEG C of freezing 30-50min, ground product particle diameter 0.3-0.5mm, it is subsequently added into the water of ground product quality 1-3 times, it is 3.5-5.5 with breast acid for adjusting pH value, at electric field intensity 25-35kV/cm under room temperature, burst length 300-500 μ s, high voltage pulse electric field processing 20-30min is carried out when pulse frequency 200-300Hz, then carry out microwave irradiation and extraction 15-20min when power 150-300W in room temperature, simultaneously at power 200-300W, when frequency 30-40KHz, carry out ultrasonic assistant extraction, adding the mixed enzyme of extracting solution quality 1.5-2.5%, in 40-50 DEG C of enzymolysis 30-50min, enzymolysis solution filters, and filtrate reduced in volume, lyophilization to moisture are that namely 5-8% obtains grape fruit powder,

5. the Fructus Vitis viniferae composite powder as described in as arbitrary in claim 1-3, it is characterized in that, the preparation method of described Wheat Sprout Powder, comprise the steps: to be placed on by Semen Tritici aestivi in the ultrasonic washing unit equipped with 0.01-0.03% sodium bicarbonate solution in power 200-400W, frequency 20-30KHz room temperature ultrasonic cleaning 3-5min, take out, drain, in frequency 2450MHz, power 3000W, temperature 38-42 DEG C, thickness of feed layer 2-4cm microwave drying 10-15s, then put temperature and be 33-36 DEG C, pH value be 6-8 soak in soak 4-6h, soak contains the compound enzyme that mass percent concentration is 0.15-0.25%, ventilate once every 15-20min, ventilation pressure 0.13-0.15MPa, simultaneously in electric field intensity 10-15kV/cm, burst length 100-300 μ s, pulse frequency 60-80Hz condition carries out high voltage pulse electric field processing, until wheat water content content is 40-45%,Semen Tritici aestivi after immersion drains, and carries out dark germination in growing floor, and dark germination temperature keeps 20-24 DEG C, and the dark germination time is 20-24h, and wheat drying to the moisture after germinateing is 5-8%, and namely low-temperature grinding, excessively 60-80 mesh sieve obtain Wheat Sprout Powder;

6. Fructus Vitis viniferae composite powder as claimed in claim 1, it is characterised in that Lactobacillus plantarum powder viable bacteria content is: 7 × 10¹²-9×10¹²cfu/g。

7. Fructus Vitis viniferae composite powder as claimed in claim 1, it is characterized in that, prepare the preparation method of cryoprotective agent during Lactobacillus plantarum powder, comprise the steps: winter rye, Caulis et Folium Ammopiptanthi Mongolici, sharkskin collagen protein is respectively washed, drain, 8-10:3-5:2-4 Homogeneous phase mixing in mass ratio, add the lactic acid moistening 3-8h that pH value is 3.8-4.5 of mixed material quality 0.1-1 times, pulverize immediately after-18--22 DEG C of freezing 1-2h, freezing thickness of feed layer 3-5cm, ground product particle diameter 0.5-3mm, it is subsequently added into the water of ground product quality 10-20 times, it is 3.5-5.5 with breast acid for adjusting pH value, at electric field intensity 25-35kV/cm under room temperature, burst length 300-500 μ s, high voltage pulse electric field processing 20-30min is carried out when pulse frequency 200-300Hz, then carry out microwave irradiation and extraction 15-20min when power 150-300W in room temperature, simultaneously at power 200-300W, when frequency 30-40KHz, carry out ultrasonic assistant extraction, add the compound enzyme of extracting solution quality 1-2%, in 45-55 DEG C of enzymolysis 30-50min, enzymolysis solution filters, filtrate concentrates, low-temperature grinding to particle diameter is that namely 0.1-0.3mm obtains protective agent,

Described compound enzyme is cellulase, protease, amylase, pectase, tannase 2-4:1-3:1-3:1-2:1-2 Homogeneous phase mixing in mass ratio.

8. the Fructus Vitis viniferae composite powder as described in as arbitrary in claim 1-3, it is characterized in that, described ferment powder preparation method is as follows: adopting the fermentation liquid that pickle fermentation produces discharge in latter stage is raw material, being filtered to remove the big grain materials such as the dish leaf in fermentation liquid and obtain fermented liquid, fermented liquid adopts lyophilization to obtain ferment powder; Cryodesiccated condition is :-30--40 degree pre-freeze 6 hours; Then lyophilizing is until water content is less than 5%.

9. the preparation method of Fructus Vitis viniferae composite powder as described in as arbitrary in claim 1-8, it is characterized in that, comprise the steps: according to formula proportion, weigh each raw material, successively ferment powder, Lactobacillus plantarum powder, grape fruit powder, Fructus Citri Limoniae powder, Wheat Sprout Powder, papaya powder are added V-type blending tank Homogeneous phase mixing 25-35min, speed of agitator 20-40r/min, be subsequently adding modified dietary fiber uniformly mixed 12-18min, speed of agitator 40-60r/min, namely sterile filling, sealing, packaging obtain Fructus Vitis viniferae composite powder.

10. the preparation method of Fructus Vitis viniferae composite powder as claimed in claim 9, it is characterized in that, the preparation method of described modified dietary fiber, comprise the following steps: by inulin, Semen Tritici aestivi fiber, Herba avenae fatuae fiber 5-7:2-4:1-3 Homogeneous phase mixing in mass ratio, add its water of quality 3-7 times, room temperature 100-300W, 35-40KHz condition supersound extraction 10-15min, then at electric field intensity 20-40kV/cm, burst length 300-500 μ s, carries out high voltage pulse electric field processing 10-15min when pulse frequency 200-400Hz; It is 4.5-6.5 with breast acid for adjusting pH value, adds the enzyme of mixture quality 0.1-0.3%, in 45-55 DEG C of enzymolysis 20-48min;Enzymolysis solution filters, and filtrate reduced in volume, lyophilization to moisture are that namely 5-8% obtains modified dietary fiber;