CN105349573A - Sorghum variety breeding and cultivating method - Google Patents
Sorghum variety breeding and cultivating method Download PDFInfo
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- CN105349573A CN105349573A CN201510906040.7A CN201510906040A CN105349573A CN 105349573 A CN105349573 A CN 105349573A CN 201510906040 A CN201510906040 A CN 201510906040A CN 105349573 A CN105349573 A CN 105349573A
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Abstract
The invention discloses a sorghum variety breeding and cultivating method. The method includes the following steps of introducing a non-toxic broad-spectrum anti-disease gene PYH157 and an insect-resistant gene CpTI into sorghum through a pollen tube channel method, selecting a positive plant, obtaining seeds of the plant, sun-drying, soaking and stirring the seeds, sowing the seeds, applying farmyard manure and base fertilizer, conducting early thinning and uniformizing to keep strong seedlings, conducting dispersing to keep the seedlings not dense but loose, pulling away sick, weak and hybrid seedlings, finally singling 2-3 seedlings in each pot according to the soil fertility, fertilizer applying level and sowing density when 5-6 leaves grow on each seedling, conducting hole-applied fertilizer dressing after the final singling, conducting the first time of intertillage soil loosening while weeding, conducting deep elongation and booting fertilizer dressing before elongation, and conducting the third time of intertillage ridging. The disease resistance of sorghum crops is improved, the growth cycle of sorghum is shortened, the maturity speed is higher, the disease resistance of the plant is high, yield is increased, quality is better, pods are plump, and plant diseases and insect pets are remarkably reduced.
Description
Technical field
The present invention relates to Chinese sorghum cultivation field, concrete a kind of sorghum variety seed selection and cultivating method thereof.
Background technology
Sorghum annual herb plant, stalk is more sturdy, uprightly, the upper tool buttress root of base portion joint; Property happiness warm, drought resisting, waterlogging; Grain sorghum, class such as sugar Chinese sorghum, broom sorghum etc. can be divided into by proterties and purposes.Grain sorghum grain is edible, wine brewing; Sugar can be refined sugar with the stalk of Chinese sorghum and be starched or eat raw; The fringe of broom sorghum can whisk broom processed or pot-scouring brush; Tender leaf dries in the shade ensiling, or dries rear capable of using as feed; Caryopsis can be used as medicine, energy removing dampness and eliminating phlegm, tranquillizing by calming the heart.Belong to cash crop.Particularly comparatively extensive at Yunnan-Guizhou Plateau mountain planting at south China, be one of this area's main food product, solve the Food Security of this area to a great extent.For improving Output of Sorghum, the implantation methods of Chinese sorghum is also multiple.The cultivation of live Chinese sorghum is wherein a kind of implantation methods, and the method is comparatively general, this method plantation Chinese sorghum that current more place is also, all general planting mostly, do not have reasonable arrangement to plant opportunity and reasonable management, cause the Output of Sorghum of planting not improve, result is unsatisfactory.
Head smut of sorghum is that Chinese sorghum endangers one of the most serious fungal disease, and time serious, sickness rate is up to more than 90%, greatly affects sorghum production.Therefore many excellent sterile systems and restorer utilize because of not anti-head smut of sorghum and to be restricted or grain is eliminated.Do not have a kind of medicament can prevention and corntrol head smut of sorghum effectively at present.Pollen tube passage method is a kind of simple foreign gene introductory technique, application of having succeeded in the genetic transformation of the crops such as cotton, paddy rice, Chinese sorghum.
Summary of the invention
For solving the problem, the invention provides a kind of sorghum variety seed selection and cultivating method thereof, by pollen tube passage method, nontoxic broad spectrum antidisease gene PYH157 and anti insect gene CpTl being imported in Chinese sorghum, coordinate rational seed treatment simultaneously, seeding method, improves the resistance against diseases of Chinese sorghum crop, and the growth cycle of Chinese sorghum is shortened, ripe speed is faster, the resistance against diseases of plant is strong, simultaneously output increased, and quality is better, pod is full, and disease and pest obviously reduces.
For achieving the above object, the technical scheme that the present invention takes is:
A kind of sorghum variety seed selection and cultivating method thereof, comprise the steps:
S1, select suitable local plantation, anti-fall, the high-quality weak, fringe is large, setting percentage is high of tillering, the sorghum variety of high yield;
S2, Agrobacterium toxalbumin VirD1, VirD2, VirE2 concentration after expression and purification is adjusted to 90-100 μ g/ μ l respectively;
After S3, use digestion with restriction enzyme nontoxic broad spectrum antidisease gene PYH157 and anti insect gene CpTl, be connected respectively on pBin438 plasmid and build two kinds of exogenous plasmid dnas;
S4, be resuspended in respectively in plasmid DNA protective material by gained two kinds of exogenous plasmid dnas, the concentration obtaining exogenous plasmid dna is the mixed solution of 1-3 μ g/ μ L;
S5, one or more the totally 2 μ l got in step S2 gained solution, add the mixed solution of the step S4 gained containing 8 μ l exogenous plasmid dnas, 6 μ l liposomes successively, after mixing, add in the damping fluid of 30 μ l, place after 2 hours for 4 DEG C, take out, by pollen tube passage method, goal gene is imported in the sorghum variety respectively selected by steps for importing S1;
S5, selection positive plant, and obtain its seed, bask seeds before broadcasting 2-3 days, by the potassium dihydrogen phosphate of the seed 0.1-0.2% of gained or with the wide withered peaceful aqua of 3-5%, 25 DEG C-40 DEG C are soaked 20-30 minute, pick up and be filtered dry rear seed dressing and carry out Dressing after Seed imbibition;
S6, choose leeward on the sunny side, ground, fertile loose vegetable garden, by Mian Ti railway carriage or compartment, 4.5-5 chi wide railway carriage or compartment, railway carriage or compartment ditch 0.5-0.8 chi, length is by the 3 jin of planting seeds in one mu of land for growing field crops, blinding thickness 1-3 centimetre, per hectare applies the farm manure 30 to 40 cubic metres becoming thoroughly decomposed, ferment, base fertilizer 160-210 kilogram, and base fertilizer is made up of the component of following weight part:
Peat composed of rotten mosses 32-38 part, methane slag 32-38 part, ammonium humate 13-22 part, diatomite 3-9 part, amino acid chelated magnesium 3-4 part, Zinc Sulphate Monohydrate 1-5 part, chelating amino acids manganese 3-5 part, chelating amino acids boron 3-4 part, poly-aspartic-acid 2-3 part, subtilis 2-3 part, thick spore Verticillium 1-3 part, print Dong element 0.15-2.5 part, actinomycin 0.1-0.3 part, bacillusmusilaginosiengineering 0.1-0.3 part, azospirillum 0.1-0.3 part.
S7, the early thinning even seedling when 3-4 leaf retains strong sprout, density stays rare, dial except sick, weak, assorted seedling, by soil fertility, Fertilization Level and thickness of sowing every nest final singling 2-3 strain during 5-6 leaf, only do every mu and ensure that 8200-13000 strain or interplanting every mu ensure 8300-9600 strain;
S8, fertilizing management: after final singling, every mu of clear excrement 20-30 load of use adds urea 2-3 kilogram, imposes and carries seed manure, carry out intertillage loosen the soil first time in conjunction with weeding, imposes group joint booting fertile before dialling joint with dark jin, and in conjunction with cultivation and banking third time.
Wherein, described damping fluid comprises 7-13mmol/L Tutofusin tris-hydrochloric acid, 0.3-0.7mmol/L diaminoethanes tetraacethyl, 7-13mmol/L sodium-chlor, 0.015 μ g μ l
-1-0.055 μ g μ l
-1ethylmethane sulfonate.
Wherein, plasmid DNA protective material is by aluminum hydroxide sol protective material, calcium phosphate protective material and Tween20 protective material 2: 3: 1 mixing gained by volume.
Wherein, restriction enzyme is restriction enzyme HindIII.+EcoRI enzyme.
Wherein, described seed dressing comprises the medium trace element of 35-65% nucleosides hydrochlorate and 35-65%, 5 '-adenylic acid (AMP) the dipotassium obtained after described nucleosides hydrochlorate comprises Yeast Nucleic Acid basic hydrolysis or sodium, 5 '-bird adenylic acid (AMP) dipotassium or sodium, 5 '-cytidylic acid dipotassium or sodium and 5 '-uridylic acid dipotassium, described medium trace element comprises Mn, B, Fe, Zn, Cu and Mo, and the weight ratio of described Mn: B: Fe: Zn: Cu: Mo is 3: 1: 2: 1: 2: 2.
The present invention has following beneficial effect:
By pollen tube passage method, nontoxic broad spectrum antidisease gene PYH157 and anti insect gene CpTl is imported in Chinese sorghum, coordinate rational seed treatment simultaneously, seeding method, improves the resistance against diseases of Chinese sorghum crop, and the growth cycle of Chinese sorghum is shortened, ripe speed is faster, the resistance against diseases of plant is strong, simultaneously output increased, and quality is better, pod is full, and disease and pest obviously reduces.
Embodiment
In order to make objects and advantages of the present invention clearly understand, below in conjunction with embodiment, the present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
In following examples, the plasmid DNA protective material used is by aluminum hydroxide sol protective material, calcium phosphate protective material and Tween20 protective material be 2: 3: 1 mixing gained by volume, the restriction enzyme used is restriction enzyme HindIII.+EcoRI enzyme, the seed dressing used comprises the medium trace element of 35-65% nucleosides hydrochlorate and 35-65%, 5 '-adenylic acid (AMP) the dipotassium obtained after described nucleosides hydrochlorate comprises Yeast Nucleic Acid basic hydrolysis or sodium, 5 '-bird adenylic acid (AMP) dipotassium or sodium, 5 '-cytidylic acid dipotassium or sodium and 5 '-uridylic acid dipotassium, described medium trace element comprises Mn, B, Fe, Zn, Cu and Mo, the weight ratio of described Mn: B: Fe: Zn: Cu: Mo is 3: 1: 2: 1: 2: 2, the damping fluid used comprises 7-13mmol/L Tutofusin tris-hydrochloric acid, 0.3-0.7mmol/L diaminoethanes tetraacethyl, 7-13mmol/L sodium-chlor, 0.015 μ g μ l
-1-0.055 μ g μ l
-1ethylmethane sulfonate.
Embodiment 1
S1, select suitable local plantation, anti-fall, the high-quality weak, fringe is large, setting percentage is high of tillering, the sorghum variety of high yield;
S2, Agrobacterium toxalbumin VirD1, VirD2, VirE2 concentration after expression and purification is adjusted to 95 μ g/ μ l respectively;
After S3, use digestion with restriction enzyme nontoxic broad spectrum antidisease gene PYH157 and anti insect gene CpTl, be connected respectively on pBin438 plasmid and build two kinds of exogenous plasmid dnas;
S4, be resuspended in respectively in plasmid DNA protective material by gained two kinds of exogenous plasmid dnas, the concentration obtaining exogenous plasmid dna is the mixed solution of 1-3 μ g/ μ L;
S5, one or more the totally 2 μ l got in step S2 gained solution, add the mixed solution of the step S4 gained containing 8 μ l exogenous plasmid dnas, 6 μ l liposomes successively, after mixing, add in the damping fluid of 30 μ l, place after 2 hours for 4 DEG C, take out, by pollen tube passage method, goal gene is imported in the sorghum variety respectively selected by steps for importing S1;
S5, select positive plant, and obtain its seed, bask seeds before broadcasting 2.5 days, by the seed of gained with 0.15% potassium dihydrogen phosphate or with the wide withered peaceful aqua of 3-5%, 32.5 DEG C of immersions 25 minutes, pick up after Seed imbibition and are filtered dry rear seed dressing and carry out Dressing;
S6, choose leeward on the sunny side, ground, fertile loose vegetable garden, by 4.75 Mian Ti railway carriage or compartments, chi wide railway carriage or compartment, railway carriage or compartment ditch 0.65 chi, length is by the 3 jin of planting seeds in one mu of land for growing field crops, blinding thickness 2 centimetres, per hectare applies the farm manure 30 to 40 cubic metres becoming thoroughly decomposed, ferment, 185 kilograms, base fertilizer, and base fertilizer is made up of the component of following weight part:
The peat composed of rotten mosses 35 parts, methane slag 35 parts, ammonium humate 17.5 parts, 6 parts, diatomite, amino acid chelated magnesium 3.5 parts, Zinc Sulphate Monohydrate 3 parts, 4 parts, chelating amino acids manganese, chelating amino acids boron 3.5 parts, poly-aspartic-acid 2.5 parts, subtilis 2.5 parts, thick spore Verticillium 2 parts, print Dong element 1.325 parts, actinomycin 0.2 part, bacillusmusilaginosiengineering 0.2 part, azospirillum 0.2 part.
S7, the early thinning even seedling when 3 leaf retains strong sprout, and density stays rare, dials except sick, weak, assorted seedling, by soil fertility, Fertilization Level and thickness of sowing every nest final singling 2-3 strain during 5 leaf, only does every mu and ensures that 10600 strains or interplanting every mu ensure 8950 strains;
S8, fertilizing management: after final singling, every mu of clear excrement 25 load of use adds 2.5 kilograms, urea, imposes and carries seed manure, carry out intertillage loosen the soil first time in conjunction with weeding, imposes group joint booting fertile before dialling joint with dark jin, and in conjunction with cultivation and banking third time.
Embodiment 2
S1, select suitable local plantation, anti-fall, the high-quality weak, fringe is large, setting percentage is high of tillering, the sorghum variety of high yield;
S2, Agrobacterium toxalbumin VirD1, VirD2, VirE2 concentration after expression and purification is adjusted to 90 μ g/ μ l respectively;
After S3, use digestion with restriction enzyme nontoxic broad spectrum antidisease gene PYH157 and anti insect gene CpTl, be connected respectively on pBin438 plasmid and build two kinds of exogenous plasmid dnas;
S4, be resuspended in respectively in plasmid DNA protective material by gained two kinds of exogenous plasmid dnas, the concentration obtaining exogenous plasmid dna is the mixed solution of 1 μ g/ μ L;
S5, one or more the totally 2 μ l got in step S2 gained solution, add the mixed solution of the step S4 gained containing 8 μ l exogenous plasmid dnas, 6 μ l liposomes successively, after mixing, add in the damping fluid of 30 μ l, place after 2 hours for 4 DEG C, take out, by pollen tube passage method, goal gene is imported in the sorghum variety respectively selected by steps for importing S1;
S5, select positive plant, and obtain its seed, bask seeds before broadcasting 2 days, by the seed of gained with 0.2% potassium dihydrogen phosphate or with 5% wide withered peaceful aqua, 25 DEG C of immersions 30 minutes, pick up after Seed imbibition and are filtered dry rear seed dressing and carry out Dressing;
S6, choose leeward on the sunny side, ground, fertile loose vegetable garden, by 4.5 Mian Ti railway carriage or compartments, chi wide railway carriage or compartment, railway carriage or compartment ditch 0.5 chi, length is by the 3 jin of planting seeds in one mu of land for growing field crops, blinding thickness 1 centimetre, per hectare applies the farm manure 30 to 40 cubic metres becoming thoroughly decomposed, ferment, 160 kilograms, base fertilizer, and base fertilizer is made up of the component of following weight part:
The peat composed of rotten mosses 32 parts, methane slag 32 parts, ammonium humate 13 parts, 3 parts, diatomite, amino acid chelated magnesium 3 parts, Zinc Sulphate Monohydrate 1 part, 3 parts, chelating amino acids manganese, chelating amino acids boron 3 parts, poly-aspartic-acid 2 parts, subtilis 2 parts, thick spore Verticillium 1 part, print Dong element 0.15 part, actinomycin 0.1 part, bacillusmusilaginosiengineering 0.1 part, azospirillum 0.1 part.
S7, the early thinning even seedling when 4 leaf retains strong sprout, and density stays rare, dials except sick, weak, assorted seedling, by soil fertility, Fertilization Level and thickness of sowing every nest final singling 2 strain during 5 leaf, only does every mu and ensures that 8200 strains or interplanting every mu ensure 8300 strains;
S8, fertilizing management: after final singling, every mu of clear excrement 20-load of use adds 2 kilograms, urea, imposes and carries seed manure, carry out intertillage loosen the soil first time in conjunction with weeding, imposes group joint booting fertile before dialling joint with dark jin, and in conjunction with cultivation and banking third time.
Embodiment 3
S1, select suitable local plantation, anti-fall, the high-quality weak, fringe is large, setting percentage is high of tillering, the sorghum variety of high yield;
S2, Agrobacterium toxalbumin VirD1, VirD2, VirE2 concentration after expression and purification is adjusted to 90-100 μ g/ μ l respectively;
After S3, use digestion with restriction enzyme nontoxic broad spectrum antidisease gene PYH157 and anti insect gene CpTl, be connected respectively on pBin438 plasmid and build two kinds of exogenous plasmid dnas;
S4, be resuspended in respectively in plasmid DNA protective material by gained two kinds of exogenous plasmid dnas, the concentration obtaining exogenous plasmid dna is the mixed solution of 3 μ g/ μ L;
S5, one or more the totally 2 μ l got in step S2 gained solution, add the mixed solution of the step S4 gained containing 8 μ l exogenous plasmid dnas, 6 μ l liposomes successively, after mixing, add in the damping fluid of 30 μ l, place after 2 hours for 4 DEG C, take out, by pollen tube passage method, goal gene is imported in the sorghum variety respectively selected by steps for importing S1;
S5, select positive plant, and obtain its seed, bask seeds before broadcasting 3 days, by the seed of gained with 0.2% potassium dihydrogen phosphate or with 5% wide withered peaceful aqua, 40 DEG C of immersions 30 minutes, pick up after Seed imbibition and are filtered dry rear seed dressing and carry out Dressing;
S6, choose leeward on the sunny side, ground, fertile loose vegetable garden, by 5 Mian Ti railway carriage or compartments, chi wide railway carriage or compartment, railway carriage or compartment ditch 0.8 chi, length is by the 3 jin of planting seeds in one mu of land for growing field crops, blinding thickness 1-3 centimetre, per hectare applies the farm manure 30 to 40 cubic metres becoming thoroughly decomposed, ferment, 210 kilograms, base fertilizer, and base fertilizer is made up of the component of following weight part:
The peat composed of rotten mosses 38 parts, methane slag 38 parts, ammonium humate 22 parts, 9 parts, diatomite, amino acid chelated magnesium 4 parts, Zinc Sulphate Monohydrate 5 parts, 5 parts, chelating amino acids manganese, chelating amino acids boron 4 parts, poly-aspartic-acid 3 parts, subtilis 3 parts, thick spore Verticillium 3 parts, print Dong element 2.5 parts, actinomycin 0.3 part, bacillusmusilaginosiengineering 0.3 part, azospirillum 0.3 part.
S7, the early thinning even seedling when 4 leaf retains strong sprout, and density stays rare, dials except sick, weak, assorted seedling, by soil fertility, Fertilization Level and thickness of sowing every nest final singling 2-3 strain during 6 leaf, only does every mu and ensures that 13000 strains or interplanting every mu ensure 9600 strains;
S8, fertilizing management: after final singling, every mu of clear excrement 30 load of use adds 3 kilograms, urea, imposes and carries seed manure, carry out intertillage loosen the soil first time in conjunction with weeding, imposes group joint booting fertile before dialling joint with dark jin, and in conjunction with cultivation and banking third time.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (5)
1. sorghum variety seed selection and a cultivating method thereof, is characterized in that, comprises the steps:
S1, select suitable local plantation, anti-fall, the high-quality weak, fringe is large, setting percentage is high of tillering, the sorghum variety of high yield;
S2, Agrobacterium toxalbumin VirD1, VirD2, VirE2 concentration after expression and purification is adjusted to 90-100 μ g/ μ l respectively;
After S3, use digestion with restriction enzyme nontoxic broad spectrum antidisease gene PYH157 and anti insect gene CpTI, be connected respectively on pBin438 plasmid and build two kinds of exogenous plasmid dnas;
S4, be resuspended in respectively in plasmid DNA protective material by gained two kinds of exogenous plasmid dnas, the concentration obtaining exogenous plasmid dna is the mixed solution of 1-3 μ g/ μ L;
S5, one or more the totally 2 μ l got in step S2 gained solution, add the mixed solution of the step S4 gained containing 8 μ l exogenous plasmid dnas, 6 μ l liposomes successively, after mixing, add in the damping fluid of 30 μ l, place after 2 hours for 4 DEG C, take out, by pollen tube passage method, goal gene is imported in the sorghum variety respectively selected by steps for importing S1;
S5, selection positive plant, and obtain its seed, bask seeds before broadcasting 2-3 days, by the potassium dihydrogen phosphate of the seed 0.1-0.2% of gained or with the wide withered peaceful aqua of 3-5%, 25 DEG C-40 DEG C are soaked 20-30 minute, pick up and be filtered dry rear seed dressing and carry out Dressing after Seed imbibition;
S6, choose leeward on the sunny side, ground, fertile loose vegetable garden, by Mian Ti railway carriage or compartment, 4.5-5 chi wide railway carriage or compartment, railway carriage or compartment ditch 0.5-0.8 chi, length is by the 3 jin of planting seeds in one mu of land for growing field crops, blinding thickness 1-3 centimetre, per hectare applies the farm manure 30 to 40 cubic metres becoming thoroughly decomposed, ferment, base fertilizer 160-210 kilogram, and base fertilizer is made up of the component of following weight part:
Peat composed of rotten mosses 32-38 part, methane slag 32-38 part, ammonium humate 13-22 part, diatomite 3-9 part, amino acid chelated magnesium 3-4 part, Zinc Sulphate Monohydrate 1-5 part, chelating amino acids manganese 3-5 part, chelating amino acids boron 3-4 part, poly-aspartic-acid 2-3 part, subtilis 2-3 part, thick spore Verticillium 1-3 part, print Dong element 0.15-2.5 part, actinomycin 0.1-0.3 part, bacillusmusilaginosiengineering 0.1-0.3 part, azospirillum 0.1-0.3 part.
S7, the early thinning even seedling when 3-4 leaf retains strong sprout, density stays rare, dial except sick, weak, assorted seedling, by soil fertility, Fertilization Level and thickness of sowing every nest final singling 2-3 strain during 5-6 leaf, only do every mu and ensure that 8200-13000 strain or interplanting every mu ensure 8300-9600 strain;
S8, fertilizing management: after final singling, every mu of clear excrement 20-30 load of use adds urea 2-3 kilogram, imposes and carries seed manure, carry out intertillage loosen the soil first time in conjunction with weeding, imposes group joint booting fertile before dialling joint with dark jin, and in conjunction with cultivation and banking third time.
2. a kind of sorghum variety seed selection according to claim 1 and cultivating method thereof, it is characterized in that, described damping fluid comprises 7-13mmol/L Tutofusin tris-hydrochloric acid, 0.3-0.7mmol/L diaminoethanes tetraacethyl, 7-13mmol/L sodium-chlor, 0.015 μ g μ l
-1-0.055 μ g μ l
-1ethylmethane sulfonate.
3. a kind of sorghum variety seed selection according to claim 1 and cultivating method thereof, is characterized in that, plasmid DNA protective material is by aluminum hydroxide sol protective material, calcium phosphate protective material and Tween20 protective material 2: 3: 1 mixing gained by volume.
4. a kind of sorghum variety seed selection according to claim 1 and cultivating method thereof, is characterized in that, restriction enzyme is restriction enzyme HindIII.+EcoRI enzyme.
5. a kind of sorghum variety seed selection according to claim 1 and cultivating method thereof, it is characterized in that, described seed dressing comprises the medium trace element of 35-65% nucleosides hydrochlorate and 35-65%, 5 '-adenylic acid (AMP) the dipotassium obtained after described nucleosides hydrochlorate comprises Yeast Nucleic Acid basic hydrolysis or sodium, 5 '-bird adenylic acid (AMP) dipotassium or sodium, 5 '-cytidylic acid dipotassium or sodium and 5 '-uridylic acid dipotassium, described medium trace element comprises Mn, B, Fe, Zn, Cu and Mo, and the weight ratio of described Mn: B: Fe: Zn: Cu: Mo is 3: 1: 2: 1: 2: 2.
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| CN106577261A (en) * | 2016-12-08 | 2017-04-26 | 山西省农业科学院高粱研究所 | Method for selecting and breeding photoperiod-sensitive sterile sweet sorghum hybrid variety by means of photoperiod-sensitive sterility |
| CN107046993A (en) * | 2017-03-28 | 2017-08-18 | 湖南中际农业科技发展有限公司 | A kind of breeding method of sorghum |
| CN107114398A (en) * | 2017-06-29 | 2017-09-01 | 山东大农药业有限公司 | A kind of dimehypo and phoxim compound Pesticidal combination |
| CN112458114A (en) * | 2020-12-25 | 2021-03-09 | 苏州农业职业技术学院 | Method for rapidly identifying functions of genes related to fruit color development of pepper fruits |
| CN113728912A (en) * | 2021-08-03 | 2021-12-03 | 贵州省旱粮研究所 | High-yield and stable-yield cultivation method for double-cropping glutinous sorghum |
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| CN106577261A (en) * | 2016-12-08 | 2017-04-26 | 山西省农业科学院高粱研究所 | Method for selecting and breeding photoperiod-sensitive sterile sweet sorghum hybrid variety by means of photoperiod-sensitive sterility |
| CN107046993A (en) * | 2017-03-28 | 2017-08-18 | 湖南中际农业科技发展有限公司 | A kind of breeding method of sorghum |
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