CN105194231A - Chinese herb feed additive for preventing bacterial gill-rot disease of fancy carps and preparation method thereof - Google Patents
Chinese herb feed additive for preventing bacterial gill-rot disease of fancy carps and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a Chinese herb feed additive for preventing a bacterial gill-rot disease of fancy carps and a preparation method thereof. The Chinese herb feed additive for preventing the bacterial gill-rot disease of the fancy carps is prepared from, by mass, 23-34 parts of onion juice, 20-28 parts of dandelion, 18-25 parts of wahlenbergia marginata, 18-25 parts of calamus, 17-25 parts of Chinese pulsatilla roots, 16-22 parts of folium artemisiae argyi, 16-22 parts of cortex mori, 12-19 parts of torilis japonica, 6-13 parts of Pu-erh ripe tea, 15-24 parts of serrate rabdosia herbs, 9-14 parts of semen oroxyli, 6-13 parts of herba violae, 8-13 parts of calyx seu fructus physalis, 6-11 parts of mint oil, 18-29 parts of dried earthworm powder, 6-12 parts of fish oil and the like. The feed additive has the good preventing effect on the bacterial gill-rot disease of the fancy carps.
Description
Technical field
The invention belongs to technical field of aquaculture, particularly relate to a kind of Chinese herbal feed additive preventing Cyprinus carpio L. bacterial gill rot disease; The invention still further relates to a kind of preparation method of Chinese herbal feed additive of above-mentioned prevention Cyprinus carpio L. bacterial gill rot disease.
Background technology
Cyprinus carpio L. (Cryprinuscarpiod) is biologically belonging to Cyprinidae (Cyprinidae), Cyprinus carpio L. is a kind of colored Cyprinus carpio, because its fish surface is bright in luster, pattern is like brocade, therefore obtain its name, Cyprinus carpio L. is the mutation of red Cyprinus carpio, it is very universal in the Ming Dynasty as pet fish that China raises red Cyprinus carpio, after red Cyprinus carpio imports Japan into, Japanese finds that in feeding process this Cyprinus carpio complexion changed can occur, according to the feature that red Cyprinus carpio easily makes a variation, through seed selection, artificial modified is red Cyprinus carpio, cultivate many new varieties, first title " color Cyprinus carpio ", " flower Cyprinus carpio ", after rename as " Cyprinus carpio L. ", Cyprinus carpio L. trip appearance is graceful, build is graceful, beautiful in colour, speckle is magnificent, be called as " in water funny fellow's stone ", enjoy the edema due to wind pathogen fish of favor and view and admire house pet.
Bacterial gill rot disease is modal a kind of disease, no matter the size of rearing conditions or fish has the possibility of generation.Its symptom is: the gill filament is pink or pale, and disorganization mucus increases then, and with mud, time serious, the endepidermis of nucleus is congested, and the epidermis of mid portion also corrodes into a slightly rounded bright zone, and be commonly called as blank, cartilage exposes; Cause disease fish dyspnea because gill filament tissue is destroyed, the nearly water meter of normal trip is in raising the nose above water to breathe shape, and the sick fish be in a bad way still has floating head phenomenon after changing clear water.Bacterial gill rot disease is popular in high temperature season usually, can cause large quantities of death of Cyprinus carpio L..Bacterial gill rot disease is caused by Cytophaga Columnaris, and carry disease germs fish and the water that carries disease germs are the sources of infection of this disease, and in this sick epidemic season, sick fish constantly scatters pathogen in water, and fish body contacts with pathogen, and especially gill portion is impaired especially easily causes infection afterwards.Easily this disease is there is in the environment that, water quality excessive at stocking density is poor.Bacterial gill rot disease is generally that spring Mo is the time of popularity to autumn in water temperature about 20 DEG C beginning.When water temperature drop reduces gradually to 15 DEG C of seasonal disease fishes.Between period of disease, the higher lethal time of raising water temperature is shorter.But treatment Cyprinus carpio L. Cyprinus carpio L. bacterial gill rot disease conventional means is cooling or sterilizes with the conventional chemical such as furazolidone, erythromycin medicine or antibiotic medicine at present.Be to solve root problem by the mode shortcoming for the treatment of of cooling, and cooling extent is too fast easily causes the generation of Cyprinus carpio L. Other diseases even to break out, in addition, summer, water temperature was difficult to remain on 15 DEG C or following.With the shortcoming of the conventional chemical such as furazolidone, erythromycin medicine or antibiotic medicine sterilization be that specific aim is poor, toxicity too greatly, Cyprinus carpio L. speedy recovery to health can not be made, easily cause water pollution and environmental disruption.Therefore, develop a kind of feedstuff of Cyprinus carpio L. bacterial gill rot disease or feed additive of effectively preventing and have important economic implications and market prospect.
Summary of the invention
An object of the present invention is to provide a kind of Chinese herbal feed additive preventing Cyprinus carpio L. bacterial gill rot disease, its raw material components is green pure natural material, good bacteriostasis and sterilization effect is had to the pathogen of Cyprinus carpio L. bacterial gill rot disease, effectively can reduce the sickness rate of Cyprinus carpio L. bacterial gill rot disease, improve immunity and the survival rate of Cyprinus carpio L..
In order to solve the problems of the technologies described above, the present invention by the following technical solutions, a kind of Chinese herbal feed additive preventing Cyprinus carpio L. bacterial gill rot disease, be made up of each component of following masses number: Bulbus Allii Cepae juice 23-34 part, Herba Taraxaci 20-28 part, Herba Wahlenbergiae 18-25 part, Rhizoma Acori Graminei 18-25 part, Radix Pulsatillae 17-25 part, Folium Artemisiae Argyi 16-22 part, Cortex Mori 16-22 part, little surreptitiously careless 12-19 part, Folium camelliae assamicae (processed) 6-13 part, Herba Rabdosiae Lophanthoidis 15-24 part, Herba Capsellae 15-21 part, Folium Isatidis 13-20 part, Herba Portulacae 11-18 part, Galla Chinensis 12-18 part, Herba Kalimeridis 11-16 part, Herba Cirsii 8-18 part, Herba Oxalidis Corniculatae 9-17 part, Herba Andrographis 8-15 part, Radix Scutellariae 7-15 part, Semen Oroxyli 9-14 part, Herba Violae 6-13 part, Calyx Seu Fructus Physalis 8-13 part, Oleum menthae 6-11 part, dry Lumbricus powder 18-29 part, fish oil 6-12 part.
Preferably, the Chinese herbal feed additive of described prevention Cyprinus carpio L. bacterial gill rot disease, be made up of each component of following masses number: Bulbus Allii Cepae juice 27-31 part, Herba Taraxaci 22-26 part, Herba Wahlenbergiae 21-23 part, Rhizoma Acori Graminei 21-23 part, Radix Pulsatillae 19-22 part, Folium Artemisiae Argyi 19-20 part, Cortex Mori 18-20 part, little surreptitiously careless 14-16 part, Folium camelliae assamicae (processed) 9-11 part, Herba Rabdosiae Lophanthoidis 18-21 part, Herba Capsellae 18-20 part, Folium Isatidis 15-17 part, Herba Portulacae 14-16 part, Galla Chinensis 14-16 part, Herba Kalimeridis 13-14 part, Herba Cirsii 12-15 part, Herba Oxalidis Corniculatae 12-14 part, Herba Andrographis 11-13 part, Radix Scutellariae 10-12 part, Semen Oroxyli 11-12 part, Herba Violae 8-10 part, Calyx Seu Fructus Physalis 9-11 part, Oleum menthae 8-9 part, dry Lumbricus powder 24-25 part, fish oil 8-9 part.
Preferably, the Chinese herbal feed additive of described prevention Cyprinus carpio L. bacterial gill rot disease, be made up of each component of following masses number: Bulbus Allii Cepae juice 29 parts, Herba Taraxaci 24 parts, Herba Wahlenbergiae 22 parts, Rhizoma Acori Graminei 22 parts, the Radix Pulsatillae 20 parts, Folium Artemisiae Argyi 20 parts, Cortex Mori 19 parts, little surreptitiously grass 15 parts, Folium camelliae assamicae (processed) 10 parts, Herba Rabdosiae Lophanthoidis 19 parts, 19 parts, Herba Capsellae, Folium Isatidis 16 parts, Herba Portulacae 15 parts, Galla Chinensis 15 parts, Herba Kalimeridis 14 parts, Herba Cirsii 13 parts, Herba Oxalidis Corniculatae 13 parts, Herba Andrographis 12 parts, Radix Scutellariae 11 parts, Semen Oroxyli 12 parts, Herba Violae 9 parts, Calyx Seu Fructus Physalis 10 parts, Oleum menthae 9 parts, dry Lumbricus powder 24 parts, 8 parts, fish oil.
Another object of the present invention is to provide a kind of preparation method of Chinese herbal feed additive of above-mentioned prevention Cyprinus carpio L. bacterial gill rot disease, and the preparation method of the Chinese herbal feed additive of this prevention Cyprinus carpio L. bacterial gill rot disease comprises the following steps:
(1) by Bulbus Allii Cepae grinding or smash or put into juice extractor to pieces and to squeeze the juice to obtain Bulbus Allii Cepae juice, and the Bulbus Allii Cepae juice of gained is taken by said ratio;
(2) Herba Taraxaci, Herba Wahlenbergiae, Rhizoma Acori Graminei, the Radix Pulsatillae, Folium Artemisiae Argyi, Cortex Mori, little surreptitiously grass and Folium camelliae assamicae (processed) is taken by proportioning, Herba Taraxaci, Herba Wahlenbergiae, Rhizoma Acori Graminei, the Radix Pulsatillae, Folium Artemisiae Argyi, Cortex Mori, little gross weight of stealing grass and Folium camelliae assamicae (processed) are denoted as 1 part, take the purified water of 20 parts, at 70-80 DEG C, 2-3h is soaked in water-bath, obtains soak;
(3) boil 2h after the soak that step (2) obtains being heated to boiling, be separated and take out fried liquid, continue the purified water adding 6 parts in medical material, continue to boil 1.5h after being heated to boiling, merge twice fried liquid and be extracting solution;
(4) dry Herba Rabdosiae Lophanthoidis, Herba Capsellae, Folium Isatidis, Herba Portulacae, Galla Chinensis, Herba Kalimeridis, Herba Cirsii, Herba Oxalidis Corniculatae, Herba Andrographis, Radix Scutellariae, Semen Oroxyli, Herba Violae and Calyx Seu Fructus Physalis is taken respectively by said ratio, load ball mill grinding 2h, cross 80 mesh sieves, obtain grounds travel;
(5) grounds travel that the extracting solution that obtains of step (3) and step (4) obtain is positioned in the extraction kettle in supercritical extraction instrument, carbon dioxide is utilized to extract as medium, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure 12Mpa, separator temperature is 70 DEG C, extracts 2 hours, is extracted liquid;
(6) dry Lumbricus powder is taken according to proportioning;
(7) take Oleum menthae and fish oil according to proportioning, mix homogeneously with the extract of step (1) load weighted Bulbus Allii Cepae juice, step (5) gained, the Lumbricus powder of step (6) load weighted drying, obtain object.
The preparation method of the Lumbricus powder of described drying is: cleaned by Lumbricus fresh water, then uses distilled water flushing three times, grinds to form powdery after finish-drying, crosses 80 mesh sieves.Further, by the method for Lumbricus finish-drying be: Lumbricus is dried or vacuum lyophilization, be preferably vacuum lyophilization.
Using method of the present invention is: throw something and feed after being mixed homogeneously with the weight ratio of 1:40-1:10 with commercially available normal feedstuff by the Chinese herbal feed additive of prevention Cyprinus carpio L. bacterial gill rot disease of the present invention, preferably, the Chinese herbal feed additive of prevention Cyprinus carpio L. bacterial gill rot disease of the present invention and the weight ratio of commercially available normal feedstuff are 1:20.
Each component effect of the present invention is resolved as follows:
Bulbus Allii Cepae is a kind of very common cheap home cooking, and the nutritional labeling in Bulbus Allii Cepae is very abundant, is not only rich in potassium, vitamin C, folic acid, zinc, selenium, and the nutrient such as cellulosic, more has the nutrient substance that two kinds special: Quercetin and prostaglandin A; Phytocide is contained as garlicin etc. in Bulbus Allii Cepae, there is very strong sterilizing ability, can effectively resist influenza virus, preventing cold, when the bacteriocidin of Bulbus Allii Cepae is discharged via respiratory tract, urinary tract, sweat gland, the cell duct wall of these positions can be stimulated to secrete, thus eliminate the phlegm again, diuresis, diaphoresis and the antibacterial effect such as anticorrosion.Herba Taraxaci energy bactericidal antiphlogistic, heat-clearing and toxic substances removing, diuresis eliminating stagnation.Control acute mastitis, lymphadenitis, scrofula, furuncle carbuncle toxin, acute conjunctivitis, cold, fever, acute tonsillitis, acute bronchitis, gastritis, hepatitis, cholecystitis, urinary tract infection.Herba Wahlenbergiae tonifying the spleen and stomach, lung benefiting kidney, eliminates the phlegm, parasite killing, hemostasis, and heat clearing away of inducing sweat is controlled wind heat and breathed heavily and cough, hemoptysis, epistaxis, infantile convulsion, traumatic injury.Rhizoma Acori Graminei can be dispelled epidemic disease Fructus Alpiniae Oxyphyllae, damp eliminating removing toxic substances, building body, combustion Rhizoma Acori Graminei, Folium Artemisiae Argyi, anophelifuge deinsectization, after 500 grams, Rhizoma Acori Graminei rhizome is mashed, add water 1-1.5 kilogram of infusion two hours, the stock solution of gained, is watered 3-6 kilogram after filtration, can effectively prevent and treat the insect pests such as planthopper, rice leafhopper, rice green caterpillar, aphid, red spider.The Radix Pulsatillae can treat the bleedings such as hemorrhoid.Control toxic-heat and blood stasis, pyrexial malaria cold and heat, epistaxis, internal hemorrhoid with bleeding, the research such as Lan Jiyu finds that Radix Pulsatillae decoct is better to the effect killing Entamoeba histolytica; Cao Jinghua etc. record Radix Pulsatillae Aqueous extracts with K-B disk diffusion method and have obvious inhibitory action to staphylococcus aureus, bacillus pyocyaneus, anthrax bacillus, Bacillus typhi, alpha streptococcus, group B streptococcus.Folium Artemisiae Argyi can exorcise evil spirits heal, expelling insect and relieving itching.Little grass of stealing has promoting blood circulation and detumescence, the effect of convergence parasite killing.The extract of the fat-soluble solvent such as petroleum ether, ether, ethyl acetate of Cortex Mori, to the quiet note of mice, can reduce the spontaneous activity of mice, and have anticonvulsant effect; The Cortex Mori severe edema due to hypofunction of the spleen or the above intraperitoneal injection of mice of n-butanol extract 50mg/kg, can make animal spontaneous activity reduce, and sense of touch and pain sensation reaction reduce, platycoria, and the generation that slightly can suppress mice galvanic shock; Cortex Mori water extract has inhibitory action to the swollen feet caused by Oral Administration in Rats or injection on Carrageenan and glucosan; Cortex Mori decoct has stronger inhibitory action to staphylococcus aureus; Also slight inhibitory action is had to Bacillus typhi and shigella flexneri; Cortex Mori furan A all has stronger bacteriostatic activity to staphylococcus aureus, mycobacterium; Cyclomorusin also has antifungic action.Little grass of stealing has promoting blood circulation and detumescence, the effect of convergence parasite killing.Folium camelliae assamicae (processed) includes abundant tea polyphenols, and Folium camelliae assamicae (processed) has anticancer, inhibiting bacteria and diminishing inflammation, alleviates tobacco poisoning, alleviates the effects such as heavy metal poison, can treat bacillary dysentery.Herba Rabdosiae Lophanthoidis has effect of clearing away heat-damp and promoting diuresis, jaundice eliminating damp eliminating, cool the blood dissipate blood stasis, may be used for treating acute icterohepatitis, acute cholecystitis, dysentery, enteritis, the difficulty in urination, the diseases such as traumatic injury stasis of blood pain.Herba Capsellae has the effect with spleen, diuretic, hemostasis, improving eyesight, is usually used in the diseases such as treatment postpartum hemorrhage, dysentery, edema, enteritis, gastric ulcer, cold, fever, the swollen pain of conjunctival congestion.Folium Isatidis can bactericidal antiphlogistic, cure mainly pyretic toxicity and send out the diseases such as speckle, erysipelas, laryngopharynx swelling and pain, aphtha of the mouth and tongue, sore and toxic, can be used for again expectorant heat stagnation lung, cough up phlegm yellow thick; Especially popular second encephalitis is usually used in.Herba Portulacae has clearing away heat-damp and promoting diuresis, removing toxic substances and promoting subsidence of swelling, antiinflammatory, quenches the thirst, diuresis.Galla Chinensis is cold in nature, sour in the mouth, puckery, has the effects such as pathogenic fire reducing of astringing the lung, relieving diarrhea with astringents, arresting sweating hemostasis, removing dampness sore, cures mainly the diseases such as chronic cough of deficiency lung, chronic diarrhea chronic dysentery, hemorrhoidal bleeding of having blood in stool, carbuncle sore tumefacting virus, Skin wet and rotten.Herba Kalimeridis is dry herb or the root of feverfew Herba Kalimeridis, and summer, autumn gather, using fresh herb or dry; Its acrid in the mouth, cool in nature, there is cooling blood for hemostasis, clearing away heat-damp and promoting diuresis, effect of removing toxic substances and promoting subsidence of swelling, can be used for spitting blood, epistaxis, dysentery, metrorrhagia, wound hemorrhage, jaundice, edema, stranguria with turbid discharge, flu, cough, pharyngalgia sore throat, hemorrhoid, carbuncle, erysipelas, infantile malnutrition.Little Artichoke leaf is sweet, cool in nature.Energy cooling blood for hemostasis, removing toxicity for eliminating carbuncles, clearing heat and relieving fidgetness, Herba Cirsii Herb is containing compositions such as choline, Catecholamine matter, Saponin, alkaloids, herb, containing compositions such as volatile oil, inulin, alkaloid, flavonoid, coumarin derivatives, all has inhibitory action to Diplococcus pneumoniae, Hemolytic streptococcus, diphtheria corynebacterium, typhoid fever village bacterium, bacillus pyocyaneus, dysentery bacterium, staphylococcus aureus etc.Herba Oxalidis Corniculatae all herbal medicine, energy bactericidal antiphlogistic, antipyretic diuresis, eliminating stagnated blood to disperse swelling, traumatic injury.Herba Andrographis has another name called Chun Lianqiuliu, Herba Andrographitis, Lan Helian, Radix Gentianae, golden Rhizoma et radix valerianae, Tremella aurantialba Bandoni et Zang hook, India's grass, Herba vallisneriae Spiralis etc.Annual herb plant, long 4-8 centimetre, wide 1-2.5 centimetre.Medicinal plants, has heat-clearing and toxic substances removing, antiinflammatory, reducing swelling and alleviating pain effect, and andrographolide has the effect obviously suppressing carbon powder Cl in venous blood.Creat formulation one Xin get Ping (including ester and ketone) can improve mice serum Lysozyme Levels.The root of Radix Scutellariae is used as medicine, bitter in the mouth, cold in nature, there are heat clearing and damp drying, eliminating fire and detoxication, hemostasis, the effect such as antiabortive, cure mainly the diseases such as epidemic febrile disease, upper respiratory tract infection, cough due to lung-heat, damp and hot jaundice, pneumonia, dysentery, hemoptysis, conjunctival congestion, frequent fetal movement, hypertension, carbuncle furuncle, the clinical antibiotic property of Radix Scutellariae is better than Rhizoma Coptidis, and does not develop immunity to drugs.Seed, the peel of stem of Semen Oroxyli contain baicaligenin, have antiinflammatory, antiallergic action, diuresis, function of gallbladder promoting, the effect of cholesterol reducing, contain chrysin, have cytotoxic activity to human nasal pharyngeal cancer (KB) cell in seed and peel of stem.Herba Violae taste bitter, acrid, cold, GUIXIN, lung meridian, have heat-clearing and toxic substances removing, and removing heat from blood is subsided a swelling, and the effect of clearing away heat-damp and promoting diuresis, cures mainly furuncle, carbuncle, scrofula, jaundice, dysentery, diarrhoea, conjunctival congestion, sore throat, venom.Calyx Seu Fructus Physalis is cold in nature, bitter in the mouth, cures mainly lung-heat laryngalgia, pneumonopathy, parotitis, hot hernia, stranguria with blood; External treatment skin sore swells, pemphigus, and gingival swelling and pain, has heat-clearing and toxic substances removing, and sore-throat relieving reduces phlegm, effect of diuresis; Seedling, leaf, stem, root all can be used as medicine, bitter in the mouth, cold, nontoxic; Seedling, leaf, stem are containing physagulin A0.02%, B0.002%, C (deoxidation physagulin A); Sour in the mouth, hardship, cold in nature, there are heat-clearing and toxic substances removing, diuresis function.Cough, jaundice, malaria, edema, furuncle, erysipelas etc. can be controlled; Root bitter in the mouth, cold in nature, there are heat clearing away, diuretic function, can treating malaria disease, jaundice, hernia.Oleum menthae light weight a surname is loose, has dispelling wind and heat pathogens, declares the merit of malicious rash: can be used for controlling wind heat bundle table, measles without adequate eruption; Also can treat rubella pruritus, get the effect that its rash of dispeling the wind is antipruritic.Containing the multiple medicinal ingredient such as Pheretima element, Pheretima toxolysin, xanthine, antihistaminic and vitamin B in Lumbricus body, and to Cyprinus carpio L., there is food calling effect.Fish oil can provide energy for Cyprinus carpio L., and has food calling effect.
In preparation method, crude drug purified water soaking a period of time, medical material deliquescing, cell expansion, effective ingredient is easily fried, and medical material contains starch, protein, after soaking, prevent when decocting, the starch gelatinization on medical material surface, protein coagulating, the capillary channel on blocking medical material surface, make the moisture when decocting enter not go, effective ingredient is not easily fried, affects curative effect of medication; Decoction can change the irritation effect of medicine, relatively mildly acts on Cyprinus carpio L., promotes that Cyprinus carpio L. is to the absorption of medicine; Grinding makes the starch in medical material, protein is separated out more fully at subsequent step; Supercritical extraction, immersion, concentration step retain the effective ingredient in Chinese medicine of the present invention to greatest extent.
The Chinese herbal feed additive of prevention Cyprinus carpio L. bacterial gill rot disease of the present invention, the mechanism of action according to Cyprinus carpio L. bacterial gill rot disease, the suitable Chinese herbal medicine of selection, and organically combined, there is significant cooperative synergism effect, play the function of each component to greatest extent.The component of the Chinese herbal feed additive of prevention Cyprinus carpio L. bacterial gill rot disease of the present invention is environmental protection raw material, environmental sound, has significant bactericidal and bacteriostatic effect to the pathogen Cytophaga Columnaris of bacterial gill rot disease.The Lumbricus powder contained in Chinese herbal feed additive due to prevention Cyprinus carpio L. bacterial gill rot disease of the present invention and fish oil have food calling effect, feedstuff mouthfeel can be improved to a certain extent, stimulate the taste bud of Cyprinus carpio L., Cyprinus carpio L. likes feedstuff of ingesting, therefore the composition that can strengthen Cyprinus carpio L. immunity and resistance, the pathogen Cytophaga Columnaris of bacterial gill rot disease be had to remarkable bactericidal and bacteriostatic effect in additive of the present invention can enter in Cyprinus carpio L. body, and plays it and act on accordingly.
Compared with prior art, the invention has the beneficial effects as follows:
(1) Bulbus Allii Cepae is a kind of very common cheap home cooking, and the nutritional labeling in Bulbus Allii Cepae is very abundant, is not only rich in potassium, vitamin C, folic acid, zinc, selenium, and the nutrient such as cellulosic, more has the nutrient substance that two kinds special: Quercetin and prostaglandin A; Phytocide is contained as garlicin etc. in Bulbus Allii Cepae, there is very strong sterilizing ability, can effectively resist influenza virus, preventing cold, when the bacteriocidin of Bulbus Allii Cepae is discharged via respiratory tract, urinary tract, sweat gland, the cell duct wall of these positions can be stimulated to secrete, thus eliminate the phlegm again, diuresis, diaphoresis and the antibacterial effect such as anticorrosion.Herba Taraxaci energy bactericidal antiphlogistic, heat-clearing and toxic substances removing, diuresis eliminating stagnation.Control acute mastitis, lymphadenitis, scrofula, furuncle carbuncle toxin, acute conjunctivitis, cold, fever, acute tonsillitis, acute bronchitis, gastritis, hepatitis, cholecystitis, urinary tract infection.Herba Wahlenbergiae tonifying the spleen and stomach, lung benefiting kidney, eliminates the phlegm, parasite killing, hemostasis, and heat clearing away of inducing sweat is controlled wind heat and breathed heavily and cough, hemoptysis, epistaxis, infantile convulsion, traumatic injury.Rhizoma Acori Graminei can be dispelled epidemic disease Fructus Alpiniae Oxyphyllae, damp eliminating removing toxic substances, building body, combustion Rhizoma Acori Graminei, Folium Artemisiae Argyi, anophelifuge deinsectization, after 500 grams, Rhizoma Acori Graminei rhizome is mashed, add water 1-1.5 kilogram of infusion two hours, the stock solution of gained, is watered 3-6 kilogram after filtration, can effectively prevent and treat the insect pests such as planthopper, rice leafhopper, rice green caterpillar, aphid, red spider.The Radix Pulsatillae can treat the bleedings such as hemorrhoid.Control toxic-heat and blood stasis, pyrexial malaria cold and heat, epistaxis, internal hemorrhoid with bleeding, the research such as Lan Jiyu finds that Radix Pulsatillae decoct is better to the effect killing Entamoeba histolytica; Cao Jinghua etc. record Radix Pulsatillae Aqueous extracts with K-B disk diffusion method and have obvious inhibitory action to staphylococcus aureus, bacillus pyocyaneus, anthrax bacillus, Bacillus typhi, alpha streptococcus, group B streptococcus.Folium Artemisiae Argyi can exorcise evil spirits heal, expelling insect and relieving itching.Little grass of stealing has promoting blood circulation and detumescence, the effect of convergence parasite killing.The extract of the fat-soluble solvent such as petroleum ether, ether, ethyl acetate of Cortex Mori, to the quiet note of mice, can reduce the spontaneous activity of mice, and have anticonvulsant effect; The Cortex Mori severe edema due to hypofunction of the spleen or the above intraperitoneal injection of mice of n-butanol extract 50mg/kg, can make animal spontaneous activity reduce, and sense of touch and pain sensation reaction reduce, platycoria, and the generation that slightly can suppress mice galvanic shock; Cortex Mori water extract has inhibitory action to the swollen feet caused by Oral Administration in Rats or injection on Carrageenan and glucosan; Cortex Mori decoct has stronger inhibitory action to staphylococcus aureus; Also slight inhibitory action is had to Bacillus typhi and shigella flexneri; Cortex Mori furan A all has stronger bacteriostatic activity to staphylococcus aureus, mycobacterium; Cyclomorusin also has antifungic action.Little grass of stealing has promoting blood circulation and detumescence, the effect of convergence parasite killing.Folium camelliae assamicae (processed) includes abundant tea polyphenols, and Folium camelliae assamicae (processed) has anticancer, inhibiting bacteria and diminishing inflammation, alleviates tobacco poisoning, alleviates the effects such as heavy metal poison, can treat bacillary dysentery.Herba Rabdosiae Lophanthoidis has effect of clearing away heat-damp and promoting diuresis, jaundice eliminating damp eliminating, cool the blood dissipate blood stasis, may be used for treating acute icterohepatitis, acute cholecystitis, dysentery, enteritis, the difficulty in urination, the diseases such as traumatic injury stasis of blood pain.Herba Capsellae has the effect with spleen, diuretic, hemostasis, improving eyesight, is usually used in the diseases such as treatment postpartum hemorrhage, dysentery, edema, enteritis, gastric ulcer, cold, fever, the swollen pain of conjunctival congestion.Folium Isatidis can bactericidal antiphlogistic, cure mainly pyretic toxicity and send out the diseases such as speckle, erysipelas, laryngopharynx swelling and pain, aphtha of the mouth and tongue, sore and toxic, can be used for again expectorant heat stagnation lung, cough up phlegm yellow thick; Especially popular second encephalitis is usually used in.Herba Portulacae has clearing away heat-damp and promoting diuresis, removing toxic substances and promoting subsidence of swelling, antiinflammatory, quenches the thirst, diuresis.Galla Chinensis is cold in nature, sour in the mouth, puckery, has the effects such as pathogenic fire reducing of astringing the lung, relieving diarrhea with astringents, arresting sweating hemostasis, removing dampness sore, cures mainly the diseases such as chronic cough of deficiency lung, chronic diarrhea chronic dysentery, hemorrhoidal bleeding of having blood in stool, carbuncle sore tumefacting virus, Skin wet and rotten.Herba Kalimeridis is dry herb or the root of feverfew Herba Kalimeridis, and summer, autumn gather, using fresh herb or dry; Its acrid in the mouth, cool in nature, there is cooling blood for hemostasis, clearing away heat-damp and promoting diuresis, effect of removing toxic substances and promoting subsidence of swelling, can be used for spitting blood, epistaxis, dysentery, metrorrhagia, wound hemorrhage, jaundice, edema, stranguria with turbid discharge, flu, cough, pharyngalgia sore throat, hemorrhoid, carbuncle, erysipelas, infantile malnutrition.Little Artichoke leaf is sweet, cool in nature.Energy cooling blood for hemostasis, removing toxicity for eliminating carbuncles, clearing heat and relieving fidgetness, Herba Cirsii Herb is containing compositions such as choline, Catecholamine matter, Saponin, alkaloids, herb, containing compositions such as volatile oil, inulin, alkaloid, flavonoid, coumarin derivatives, all has inhibitory action to Diplococcus pneumoniae, Hemolytic streptococcus, diphtheria corynebacterium, typhoid fever village bacterium, bacillus pyocyaneus, dysentery bacterium, staphylococcus aureus etc.Herba Oxalidis Corniculatae all herbal medicine, energy bactericidal antiphlogistic, antipyretic diuresis, eliminating stagnated blood to disperse swelling, traumatic injury.Herba Andrographis has another name called Chun Lianqiuliu, Herba Andrographitis, Lan Helian, Radix Gentianae, golden Rhizoma et radix valerianae, Tremella aurantialba Bandoni et Zang hook, India's grass, Herba vallisneriae Spiralis etc.Annual herb plant, long 4-8 centimetre, wide 1-2.5 centimetre.Medicinal plants, has heat-clearing and toxic substances removing, antiinflammatory, reducing swelling and alleviating pain effect, and andrographolide has the effect obviously suppressing carbon powder Cl in venous blood.Creat formulation one Xin get Ping (including ester and ketone) can improve mice serum Lysozyme Levels.The root of Radix Scutellariae is used as medicine, bitter in the mouth, cold in nature, there are heat clearing and damp drying, eliminating fire and detoxication, hemostasis, the effect such as antiabortive, cure mainly the diseases such as epidemic febrile disease, upper respiratory tract infection, cough due to lung-heat, damp and hot jaundice, pneumonia, dysentery, hemoptysis, conjunctival congestion, frequent fetal movement, hypertension, carbuncle furuncle, the clinical antibiotic property of Radix Scutellariae is better than Rhizoma Coptidis, and does not develop immunity to drugs.Seed, the peel of stem of Semen Oroxyli contain baicaligenin, have antiinflammatory, antiallergic action, diuresis, function of gallbladder promoting, the effect of cholesterol reducing, contain chrysin, have cytotoxic activity to human nasal pharyngeal cancer (KB) cell in seed and peel of stem.Herba Violae taste bitter, acrid, cold, GUIXIN, lung meridian, have heat-clearing and toxic substances removing, and removing heat from blood is subsided a swelling, and the effect of clearing away heat-damp and promoting diuresis, cures mainly furuncle, carbuncle, scrofula, jaundice, dysentery, diarrhoea, conjunctival congestion, sore throat, venom.Calyx Seu Fructus Physalis is cold in nature, bitter in the mouth, cures mainly lung-heat laryngalgia, pneumonopathy, parotitis, hot hernia, stranguria with blood; External treatment skin sore swells, pemphigus, and gingival swelling and pain, has heat-clearing and toxic substances removing, and sore-throat relieving reduces phlegm, effect of diuresis; Seedling, leaf, stem, root all can be used as medicine, bitter in the mouth, cold, nontoxic; Seedling, leaf, stem are containing physagulin A0.02%, B0.002%, C (deoxidation physagulin A); Sour in the mouth, hardship, cold in nature, there are heat-clearing and toxic substances removing, diuresis function.Cough, jaundice, malaria, edema, furuncle, erysipelas etc. can be controlled; Root bitter in the mouth, cold in nature, there are heat clearing away, diuretic function, can treating malaria disease, jaundice, hernia.Oleum menthae light weight a surname is loose, has dispelling wind and heat pathogens, declares the merit of malicious rash: can be used for controlling wind heat bundle table, measles without adequate eruption; Also can treat rubella pruritus, get the effect that its rash of dispeling the wind is antipruritic.Containing the multiple medicinal ingredient such as Pheretima element, Pheretima toxolysin, xanthine, antihistaminic and vitamin B in Lumbricus body, and to Cyprinus carpio L., there is food calling effect.Fish oil can provide energy for Cyprinus carpio L., and has food calling effect.
(2) Chinese herbal feed additive of prevention Cyprinus carpio L. bacterial gill rot disease of the present invention, the mechanism of action according to Cyprinus carpio L. bacterial gill rot disease, the suitable Chinese herbal medicine of selection, and organically combined, there is significant cooperative synergism effect, play the function of each component to greatest extent.The component of the Chinese herbal feed additive of prevention Cyprinus carpio L. bacterial gill rot disease of the present invention is environmental protection raw material, environmental sound, has significant bactericidal and bacteriostatic effect to the pathogen Cytophaga Columnaris of bacterial gill rot disease.The Lumbricus powder contained in Chinese herbal feed additive due to prevention Cyprinus carpio L. bacterial gill rot disease of the present invention and fish oil have food calling effect, feedstuff mouthfeel can be improved to a certain extent, stimulate the taste bud of Cyprinus carpio L., Cyprinus carpio L. likes feedstuff of ingesting, and the composition that can strengthen Cyprinus carpio L. immunity and resistance, the pathogen Cytophaga Columnaris of bacterial gill rot disease be had to remarkable bactericidal and bacteriostatic effect therefore in additive of the present invention to enter in Cyprinus carpio L. body and to play it and act on accordingly.
(3) in preparation method, crude drug purified water soaking a period of time, medical material deliquescing, cell expansion, effective ingredient is easily fried, and medical material contains starch, protein, after soaking, prevent when decocting, the starch gelatinization on medical material surface, protein coagulating, the capillary channel on blocking medical material surface, make the moisture when decocting enter not go, effective ingredient is not easily fried, affects curative effect of medication; Decoction can change the irritation effect of medicine, relatively mildly acts on Cyprinus carpio L., promotes that Cyprinus carpio L. is to the absorption of medicine; Grinding makes the starch in medical material, protein is separated out more fully at subsequent step; Supercritical extraction, immersion, concentration step retain the effective ingredient in Chinese medicine of the present invention to greatest extent.
Detailed description of the invention
Following embodiment is convenient to understand the present invention better, but does not limit the present invention.Experimental technique in following embodiment, if no special instructions, is conventional method.
Embodiment 1
In the present invention's first detailed description of the invention, feed ingredient and preparation method thereof is as follows:
A kind of Chinese herbal feed additive preventing Cyprinus carpio L. bacterial gill rot disease, be made up of each component of following masses number: Bulbus Allii Cepae juice 23 parts, Herba Taraxaci 20 parts, Herba Wahlenbergiae 19 parts, Rhizoma Acori Graminei 18 parts, the Radix Pulsatillae 18 parts, Folium Artemisiae Argyi 16 parts, Cortex Mori 16 parts, little surreptitiously grass 19 parts, Folium camelliae assamicae (processed) 13 parts, Herba Rabdosiae Lophanthoidis 15 parts, 21 parts, Herba Capsellae, Folium Isatidis 13 parts, Herba Portulacae 16 parts, Galla Chinensis 12 parts, Herba Kalimeridis 12 parts, Herba Cirsii 8 parts, Herba Oxalidis Corniculatae 9 parts, Herba Andrographis 10 parts, Radix Scutellariae 7 parts, Semen Oroxyli 9 parts, Herba Violae 8 parts, Calyx Seu Fructus Physalis 9 parts, Oleum menthae 6 parts, dry Lumbricus powder 18 parts, 7 parts, fish oil.
The preparation method of the Chinese herbal feed additive of above-mentioned prevention Cyprinus carpio L. bacterial gill rot disease comprises the following steps:
(3) by Bulbus Allii Cepae grinding or smash or put into juice extractor to pieces and to squeeze the juice to obtain Bulbus Allii Cepae juice, and the Bulbus Allii Cepae juice of gained is taken by said ratio;
(4) Herba Taraxaci, Herba Wahlenbergiae, Rhizoma Acori Graminei, the Radix Pulsatillae, Folium Artemisiae Argyi, Cortex Mori, little surreptitiously grass and Folium camelliae assamicae (processed) is taken by proportioning, Herba Taraxaci, Herba Wahlenbergiae, Rhizoma Acori Graminei, the Radix Pulsatillae, Folium Artemisiae Argyi, Cortex Mori, little gross weight of stealing grass and Folium camelliae assamicae (processed) are denoted as 1 part, take the purified water of 20 parts, at 70-80 DEG C, 2-3h is soaked in water-bath, obtains soak;
(3) boil 2h after the soak that step (2) obtains being heated to boiling, be separated and take out fried liquid, continue the purified water adding 6 parts in medical material, continue to boil 1.5h after being heated to boiling, merge twice fried liquid and be extracting solution;
(4) dry Herba Rabdosiae Lophanthoidis, Herba Capsellae, Folium Isatidis, Herba Portulacae, Galla Chinensis, Herba Kalimeridis, Herba Cirsii, Herba Oxalidis Corniculatae, Herba Andrographis, Radix Scutellariae, Semen Oroxyli, Herba Violae and Calyx Seu Fructus Physalis is taken respectively by said ratio, load ball mill grinding 2h, cross 80 mesh sieves, obtain grounds travel;
(5) grounds travel that the extracting solution that obtains of step (3) and step (4) obtain is positioned in the extraction kettle in supercritical extraction instrument, carbon dioxide is utilized to extract as medium, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure 12Mpa, separator temperature is 70 DEG C, extracts 2 hours, is extracted liquid;
(6) dry Lumbricus powder is taken according to proportioning;
(7) take Oleum menthae and fish oil according to proportioning, mix homogeneously with the extract of step (1) load weighted Bulbus Allii Cepae juice, step (5) gained, the Lumbricus powder of step (6) load weighted drying, obtain object.
The preparation method of the Lumbricus powder of described drying is: cleaned by Lumbricus fresh water, then uses distilled water flushing three times, grinds to form powdery after vacuum lyophilization to finish-drying, crosses 80 mesh sieves.
Embodiment 2
In the present invention's second detailed description of the invention, feed ingredient and preparation method thereof is as follows:
A kind of Chinese herbal feed additive preventing Cyprinus carpio L. bacterial gill rot disease, be made up of each component of following masses number: Bulbus Allii Cepae juice 31 parts, Herba Taraxaci 28 parts, Herba Wahlenbergiae 25 parts, Rhizoma Acori Graminei 23 parts, the Radix Pulsatillae 22 parts, Folium Artemisiae Argyi 22 parts, Cortex Mori 20 parts, little surreptitiously grass 16 parts, Folium camelliae assamicae (processed) 11 parts, Herba Rabdosiae Lophanthoidis 21 parts, 20 parts, Herba Capsellae, Folium Isatidis 20 parts, Herba Portulacae 18 parts, Galla Chinensis 18 parts, Herba Kalimeridis 16 parts, Herba Cirsii 15 parts, Herba Oxalidis Corniculatae 17 parts, Herba Andrographis 15 parts, Radix Scutellariae 15 parts, Semen Oroxyli 14 parts, Herba Violae 13 parts, Calyx Seu Fructus Physalis 11 parts, Oleum menthae 11 parts, dry Lumbricus powder 26 parts, 6 parts, fish oil.
The preparation method of the Chinese herbal feed additive of the prevention Cyprinus carpio L. bacterial gill rot disease of the present embodiment is with embodiment 1.
Embodiment 3
In the present invention's the 3rd detailed description of the invention, feed ingredient and preparation method thereof is as follows:
A kind of Chinese herbal feed additive preventing Cyprinus carpio L. bacterial gill rot disease, be made up of each component of following masses number: Bulbus Allii Cepae juice 34 parts, Herba Taraxaci 26 parts, Herba Wahlenbergiae 23 parts, Rhizoma Acori Graminei 25 parts, the Radix Pulsatillae 25 parts, Folium Artemisiae Argyi 20 parts, Cortex Mori 22 parts, little surreptitiously grass 14 parts, Folium camelliae assamicae (processed) 9 parts, Herba Rabdosiae Lophanthoidis 18 parts, 18 parts, Herba Capsellae, Folium Isatidis 17 parts, Herba Portulacae 14 parts, Galla Chinensis 16 parts, Herba Kalimeridis 14 parts, Herba Cirsii 18 parts, Herba Oxalidis Corniculatae 14 parts, Herba Andrographis 13 parts, Radix Scutellariae 12 parts, Semen Oroxyli 12 parts, Herba Violae 10 parts, Calyx Seu Fructus Physalis 13 parts, Oleum menthae 9 parts, dry Lumbricus powder 28 parts, 8 parts, fish oil.
The preparation method of the Chinese herbal feed additive of the prevention Cyprinus carpio L. bacterial gill rot disease of the present embodiment is with embodiment 1.
Embodiment 4
In the present invention's the 4th detailed description of the invention, feed ingredient and preparation method thereof is as follows:
A kind of Chinese herbal feed additive preventing Cyprinus carpio L. bacterial gill rot disease, be made up of each component of following masses number: Bulbus Allii Cepae juice 27 parts, Herba Taraxaci 22 parts, Herba Wahlenbergiae 21 parts, Rhizoma Acori Graminei 21 parts, the Radix Pulsatillae 17 parts, Folium Artemisiae Argyi 19 parts, Cortex Mori 18 parts, little surreptitiously grass 12 parts, Folium camelliae assamicae (processed) 6 parts, Herba Rabdosiae Lophanthoidis 24 parts, 15 parts, Herba Capsellae, Folium Isatidis 15 parts, Herba Portulacae 11 parts, Galla Chinensis 14 parts, Herba Kalimeridis 13 parts, Herba Cirsii 12 parts, Herba Oxalidis Corniculatae 12 parts, Herba Andrographis 8 parts, Radix Scutellariae 10 parts, Semen Oroxyli 11 parts, Herba Violae 6 parts, Calyx Seu Fructus Physalis 8 parts, Oleum menthae 8 parts, dry Lumbricus powder 29 parts, 12 parts, fish oil.
The preparation method of the Chinese herbal feed additive of the prevention Cyprinus carpio L. bacterial gill rot disease of the present embodiment is with embodiment 1.
Embodiment 5
In the present invention's the 5th detailed description of the invention, feed ingredient and preparation method thereof is as follows:
A kind of Chinese herbal feed additive preventing Cyprinus carpio L. bacterial gill rot disease, be made up of each component of following masses number: Bulbus Allii Cepae juice 28 parts, Herba Taraxaci 22 parts, Herba Wahlenbergiae 18 parts, Rhizoma Acori Graminei 21 parts, the Radix Pulsatillae 19 parts, Folium Artemisiae Argyi 16 parts, Cortex Mori 20 parts, little surreptitiously grass 16 parts, Folium camelliae assamicae (processed) 11 parts, Herba Rabdosiae Lophanthoidis 18 parts, 15 parts, Herba Capsellae, Folium Isatidis 13 parts, Herba Portulacae 11 parts, Galla Chinensis 15 parts, Herba Kalimeridis 11 parts, Herba Cirsii 16 parts, Herba Oxalidis Corniculatae 13 parts, Herba Andrographis 11 parts, Radix Scutellariae 7 parts, Semen Oroxyli 11 parts, Herba Violae 10 parts, Calyx Seu Fructus Physalis 11 parts, Oleum menthae 9 parts, dry Lumbricus powder 25 parts, 9 parts, fish oil.
The preparation method of the Chinese herbal feed additive of the prevention Cyprinus carpio L. bacterial gill rot disease of the present embodiment is with embodiment 1.
Embodiment 6
In the present invention's the 6th detailed description of the invention, feed ingredient and preparation method thereof is as follows:
A kind of Chinese herbal feed additive preventing Cyprinus carpio L. bacterial gill rot disease, be made up of each component of following masses number: Bulbus Allii Cepae juice 29 parts, Herba Taraxaci 24 parts, Herba Wahlenbergiae 22 parts, Rhizoma Acori Graminei 22 parts, the Radix Pulsatillae 20 parts, Folium Artemisiae Argyi 20 parts, Cortex Mori 19 parts, little surreptitiously grass 15 parts, Folium camelliae assamicae (processed) 10 parts, Herba Rabdosiae Lophanthoidis 19 parts, 19 parts, Herba Capsellae, Folium Isatidis 16 parts, Herba Portulacae 15 parts, Galla Chinensis 15 parts, Herba Kalimeridis 14 parts, Herba Cirsii 13 parts, Herba Oxalidis Corniculatae 13 parts, Herba Andrographis 12 parts, Radix Scutellariae 11 parts, Semen Oroxyli 12 parts, Herba Violae 9 parts, Calyx Seu Fructus Physalis 10 parts, Oleum menthae 9 parts, dry Lumbricus powder 24 parts, 8 parts, fish oil.
The preparation method of the Chinese herbal feed additive of the prevention Cyprinus carpio L. bacterial gill rot disease of the present embodiment is with embodiment 1.
Experimental result
Culture experiment
Select normal feedstuff (for normal commercial diet) be matched group, to add the feedstuff group of the embodiment of the present invention 6 in normal feedstuff for experimental group, often group establish 3 parallel, in experimental group, the additive of embodiment 6 and the weight ratio of normal feedstuff are 1:20.Choose 6 aquariums, healthy Cyprinus carpio L. 300, healthy Cyprinus carpio L. is all assigned to 6 aquariums at random, each aquarium 50 Cyprinus carpio L., add the Cyprinus carpio L. that 5 are suffered from bacterial gill rot disease in each aquarium.Observe the growth of Cyprinus carpio L. and dead, infection conditions in feeding process, observe 21 days, experimental result is in table 1:
The healthy rate of table 1 experimental group Cyprinus carpio L. and matched group Cyprinus carpio L., mortality rate and infection rate
| Group | Healthy rate | Mortality rate | Infection rate |
| Matched group | 54％ | 30％ | 46％ |
| Experimental group | 82％ | 4％ | 18％ |
From table 1, the Chinese herbal feed additive of prevention Cyprinus carpio L. bacterial gill rot disease of the present invention can effectively reduce infection rate and mortality rate, and the Chinese herbal feed additive of prevention Cyprinus carpio L. bacterial gill rot disease of the present invention has good preventive effect.
Bacteriostatic test checks
Adopt furazolidone to do matched group, the Chinese herbal feed additive of the prevention Cyprinus carpio L. bacterial gill rot disease of embodiment 6 is that experimental group carries out antibacterial inspection.
The preparation of culture medium: take in the water-soluble 800mL of beef extract 5g, adds peptone 10g and dissolves, and adds casein 34g and dissolves, then adds NaCl5g dissolving, is adjusted to pH7.6 ~ 7.8, is diluted to 1000mL.Add agar powder 18g, autoclaving 15min, when being cooled to 40 DEG C ~ 50 DEG C, a kind of rhyme scheme in Chinese operas serving as the prelude to a complete score for voices, solidifies, and the 4 DEG C of refrigerators placing no bacteria pollution are for subsequent use.
Strain is: Cytophaga Columnaris.
Matched group antibacterial liquid is prepared: be dissolved in respectively in 10ml sterile deionized water by 1 part of furazolidone, diluted respectively by liquor strength, with stock solution than being 1:2,1:4,1:8,1:16,1:32.
Experimental group antibacterial liquid is prepared: be dissolved in 10ml sterile deionized water by the additive of embodiments of the invention 6, diluted respectively by liquor strength, with stock solution than being 1:2,1:4,1:8,1:16,1:32.Cytophaga Columnaris result fungistatic effect is in table 2:
The bactericidal and bacteriostatic effect of the Chinese herbal feed additive of table 2 prevention Cyprinus carpio L. of the present invention bacterial gill rot disease
From table 2, the Chinese herbal feed additive of prevention Cyprinus carpio L. bacterial gill rot disease of the present invention has good bactericidal and bacteriostatic effect.
The above is only the preferred embodiment of the present invention, is noted that for those skilled in the art, and under the premise without departing from the principles of the invention, can also make some improvement, these improvement should be considered as protection scope of the present invention.
Claims (8)
1. one kind is prevented the Chinese herbal feed additive of Cyprinus carpio L. bacterial gill rot disease, it is characterized in that: be made up of each component of following masses number: Bulbus Allii Cepae juice 23-34 part, Herba Taraxaci 20-28 part, Herba Wahlenbergiae 18-25 part, Rhizoma Acori Graminei 18-25 part, Radix Pulsatillae 17-25 part, Folium Artemisiae Argyi 16-22 part, Cortex Mori 16-22 part, little surreptitiously careless 12-19 part, Folium camelliae assamicae (processed) 6-13 part, Herba Rabdosiae Lophanthoidis 15-24 part, Herba Capsellae 15-21 part, Folium Isatidis 13-20 part, Herba Portulacae 11-18 part, Galla Chinensis 12-18 part, Herba Kalimeridis 11-16 part, Herba Cirsii 8-18 part, Herba Oxalidis Corniculatae 9-17 part, Herba Andrographis 8-15 part, Radix Scutellariae 7-15 part, Semen Oroxyli 9-14 part, Herba Violae 6-13 part, Calyx Seu Fructus Physalis 8-13 part, Oleum menthae 6-11 part, dry Lumbricus powder 18-29 part, fish oil 6-12 part.
2. the Chinese herbal feed additive of prevention Cyprinus carpio L. bacterial gill rot disease according to claim 1, it is characterized in that: the Chinese herbal feed additive of described prevention Cyprinus carpio L. bacterial gill rot disease, be made up of each component of following masses number: Bulbus Allii Cepae juice 27-31 part, Herba Taraxaci 22-26 part, Herba Wahlenbergiae 21-23 part, Rhizoma Acori Graminei 21-23 part, Radix Pulsatillae 19-22 part, Folium Artemisiae Argyi 19-20 part, Cortex Mori 18-20 part, little surreptitiously careless 14-16 part, Folium camelliae assamicae (processed) 9-11 part, Herba Rabdosiae Lophanthoidis 18-21 part, Herba Capsellae 18-20 part, Folium Isatidis 15-17 part, Herba Portulacae 14-16 part, Galla Chinensis 14-16 part, Herba Kalimeridis 13-14 part, Herba Cirsii 12-15 part, Herba Oxalidis Corniculatae 12-14 part, Herba Andrographis 11-13 part, Radix Scutellariae 10-12 part, Semen Oroxyli 11-12 part, Herba Violae 8-10 part, Calyx Seu Fructus Physalis 9-11 part, Oleum menthae 8-9 part, dry Lumbricus powder 24-25 part, fish oil 8-9 part.
3. the Chinese herbal feed additive of prevention Cyprinus carpio L. bacterial gill rot disease according to claim 1, it is characterized in that: the Chinese herbal feed additive of described prevention Cyprinus carpio L. bacterial gill rot disease, be made up of each component of following masses number: Bulbus Allii Cepae juice 29 parts, Herba Taraxaci 24 parts, Herba Wahlenbergiae 22 parts, Rhizoma Acori Graminei 22 parts, the Radix Pulsatillae 20 parts, Folium Artemisiae Argyi 20 parts, Cortex Mori 19 parts, little surreptitiously grass 15 parts, Folium camelliae assamicae (processed) 10 parts, Herba Rabdosiae Lophanthoidis 19 parts, 19 parts, Herba Capsellae, Folium Isatidis 16 parts, Herba Portulacae 15 parts, Galla Chinensis 15 parts, Herba Kalimeridis 14 parts, Herba Cirsii 13 parts, Herba Oxalidis Corniculatae 13 parts, Herba Andrographis 12 parts, Radix Scutellariae 11 parts, Semen Oroxyli 12 parts, Herba Violae 9 parts, Calyx Seu Fructus Physalis 10 parts, Oleum menthae 9 parts, dry Lumbricus powder 24 parts, 8 parts, fish oil.
4. a preparation method for the Chinese herbal feed additive of the prevention Cyprinus carpio L. bacterial gill rot disease according to any one of claim 1-3, is characterized in that: comprise the following steps:
(1) by Bulbus Allii Cepae grinding or smash or put into juice extractor to pieces and to squeeze the juice to obtain Bulbus Allii Cepae juice, and the Bulbus Allii Cepae juice of gained is taken by said ratio;
(2) Herba Taraxaci, Herba Wahlenbergiae, Rhizoma Acori Graminei, the Radix Pulsatillae, Folium Artemisiae Argyi, Cortex Mori, little surreptitiously grass and Folium camelliae assamicae (processed) is taken by proportioning, Herba Taraxaci, Herba Wahlenbergiae, Rhizoma Acori Graminei, the Radix Pulsatillae, Folium Artemisiae Argyi, Cortex Mori, little gross weight of stealing grass and Folium camelliae assamicae (processed) are denoted as 1 part, take the purified water of 20 parts, at 70-80 DEG C, 2-3h is soaked in water-bath, obtains soak;
(3) boil 2h after the soak that step (2) obtains being heated to boiling, be separated and take out fried liquid, continue the purified water adding 6 parts in medical material, continue to boil 1.5h after being heated to boiling, merge twice fried liquid and be extracting solution;
(4) dry Herba Rabdosiae Lophanthoidis, Herba Capsellae, Folium Isatidis, Herba Portulacae, Galla Chinensis, Herba Kalimeridis, Herba Cirsii, Herba Oxalidis Corniculatae, Herba Andrographis, Radix Scutellariae, Semen Oroxyli, Herba Violae and Calyx Seu Fructus Physalis is taken respectively by said ratio, load ball mill grinding 2h, cross 80 mesh sieves, obtain grounds travel;
(5) grounds travel that the extracting solution that obtains of step (3) and step (4) obtain is positioned in the extraction kettle in supercritical extraction instrument, carbon dioxide is utilized to extract as medium, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure 12Mpa, separator temperature is 70 DEG C, extracts 2 hours, is extracted liquid;
(6) dry Lumbricus powder is taken according to proportioning;
(7) take Oleum menthae and fish oil according to proportioning, mix homogeneously with the extract of step (1) load weighted Bulbus Allii Cepae juice, step (5) gained, the Lumbricus powder of step (6) load weighted drying, obtain object.
5. the preparation method of the Chinese herbal feed additive of prevention Cyprinus carpio L. bacterial gill rot disease according to claim 4, it is characterized in that: the preparation method of the Lumbricus powder of described drying is: cleaned by Lumbricus fresh water, use distilled water flushing again three times, dry and grind to form powdery to finish-drying, cross 80 mesh sieves.
6. the preparation method of the Chinese herbal feed additive of prevention Cyprinus carpio L. bacterial gill rot disease according to claim 4, it is characterized in that: the preparation method of the Lumbricus powder of described drying is: cleaned by Lumbricus fresh water, use distilled water flushing again three times, grind to form powdery after vacuum lyophilization to finish-drying, cross 80 mesh sieves.
7. a using method for the Chinese herbal feed additive of the prevention Cyprinus carpio L. bacterial gill rot disease according to any one of claim 1-3, is characterized in that: throw something and feed after being mixed homogeneously with the weight ratio of 1:40-1:10 with commercially available normal feedstuff by the Chinese herbal feed additive of prevention Cyprinus carpio L. bacterial gill rot disease of the present invention.
8. the using method of the Chinese herbal feed additive of prevention Cyprinus carpio L. bacterial gill rot disease according to claim 7, is characterized in that: the Chinese herbal feed additive of prevention Cyprinus carpio L. bacterial gill rot disease of the present invention and the weight ratio of commercially available normal feedstuff are 1:20.
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| CN201510584940.4A Pending CN105194231A (en) | 2015-09-15 | 2015-09-15 | Chinese herb feed additive for preventing bacterial gill-rot disease of fancy carps and preparation method thereof |
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| CN (1) | CN105194231A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106616028A (en) * | 2016-12-08 | 2017-05-10 | 西南大学 | Viral disease preventing and curing feed additive for cyprinus carpio and preparation method thereof |
| CN107048094A (en) * | 2017-06-02 | 2017-08-18 | 贵州江口绿源水产发展有限公司 | A kind of bait and its processing method for preventing and treating carp fin rot |
| CN109125573A (en) * | 2018-11-20 | 2019-01-04 | 黄山市鼎新生态农业发展有限公司 | A kind of Chinese medicine composition and preparation method thereof for treating light lip fish bacterial gill |
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| JP2010095529A (en) * | 2009-12-22 | 2010-04-30 | Maruzen Pharmaceut Co Ltd | Composition for improving lipid metabolism |
| CN104013740A (en) * | 2014-06-19 | 2014-09-03 | 湖州天健兽药有限公司 | Traditional Chinese medicine for treating viral hemorrhagic disease of grass carp |
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| JP2010095529A (en) * | 2009-12-22 | 2010-04-30 | Maruzen Pharmaceut Co Ltd | Composition for improving lipid metabolism |
| CN104013740A (en) * | 2014-06-19 | 2014-09-03 | 湖州天健兽药有限公司 | Traditional Chinese medicine for treating viral hemorrhagic disease of grass carp |
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| 刘松岩等: "常见中草药的药理作用及其在鱼病防治中的应用", 《信阳农业高等专科学校学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106616028A (en) * | 2016-12-08 | 2017-05-10 | 西南大学 | Viral disease preventing and curing feed additive for cyprinus carpio and preparation method thereof |
| CN107048094A (en) * | 2017-06-02 | 2017-08-18 | 贵州江口绿源水产发展有限公司 | A kind of bait and its processing method for preventing and treating carp fin rot |
| CN109125573A (en) * | 2018-11-20 | 2019-01-04 | 黄山市鼎新生态农业发展有限公司 | A kind of Chinese medicine composition and preparation method thereof for treating light lip fish bacterial gill |
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