CN105327318A - Feed additive for preventing and treating bacterial gill-rot disease of ictalurus punctatus and preparation method of feed additive - Google Patents
Feed additive for preventing and treating bacterial gill-rot disease of ictalurus punctatus and preparation method of feed additive Download PDFInfo
- Publication number
- CN105327318A CN105327318A CN201510845850.6A CN201510845850A CN105327318A CN 105327318 A CN105327318 A CN 105327318A CN 201510845850 A CN201510845850 A CN 201510845850A CN 105327318 A CN105327318 A CN 105327318A
- Authority
- CN
- China
- Prior art keywords
- parts
- juice
- feed additive
- rot disease
- powder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention belongs to the technical field of aquaculture, and relates to a feed additive for preventing and treating bacterial gill-rot disease of ictalurus punctatus and a preparation method of the feed additive for preventing and treating the bacterial gill-rot disease of the ictalurus punctatus. The feed additive for preventing and treating the bacterial gill-rot disease of the ictalurus punctatus is prepared from the following components in parts by mass: 35 to 45 parts of ginger juice, 26 to 32 parts of lalang grass rhizome, 26 to 32 parts of cactus juice, 25 to 33 parts of acorus calamus, 25 to 32 parts of onion juice, 22 to 30 parts of portulaca oleracea, 22 to 28 parts of dark plum, 22 to 28 parts of dried orange peel, 20 to 28 parts of Chinese gallnut, 18 to 28 parts of almond powder, 16 to 26 parts of euphorbia helioscopia, 16 to 26 parts of acanthaceae sericocalyx chinensis, 10 to 18 parts of fish oil and the like. According to the feed additive and the preparation method disclosed by the invention, the occurrence of the bacterial gill-rot disease of the ictalurus punctatus can be effectively prevented; in addition, the bacterial gill-rot disease of the ictalurus punctatus can be effectively treated.
Description
Technical field
The invention belongs to technical field of aquaculture, particularly relate to a kind of feed additive preventing and treating Ietalurus Punetaus bacterial gill rot disease; The invention still further relates to a kind of preparation method of feed additive of above-mentioned control Ietalurus Punetaus bacterial gill rot disease.
Background technology
(IctalurusPunctatus (Rafinesque), also known as ditch Nian (ChannelCatfish), belongs to Silurformes (Siluriformes), Channel-catfish section (Ictaluridae) Fish to Ietalurus Punetaus.Ietalurus Punetaus natural distribution area in cen.am. basin, southern Canada and the Atlantic coast Electric Field At Ground In Partial Region, enter the Atlantic coast widely later, entire United States and northern Mexico have distribution, its place of production is water nonpollution, sandy or chad substrate, flow velocity big-and-middle river faster, also can enter the life of salt-fresh water waters.It is now one of main freshwater aquiculture kind of the U.S..Ietalurus Punetaus is the Channel-catfish section Fish that Hubei Aquatic Scienctific Research Inst. introduced in 1984 with cloud speckle Channel-catfish simultaneously, through research in a few years and popularization cultivation, confirms that this kind of fish is applicable to the most regional cultivation of China.
The pathogen of Ietalurus Punetaus bacterial gill rot disease is flavobacterium columnare, and Ietalurus Punetaus is more responsive to bacterial gill rot disease.The principal pathogenetic symptom of this disease is: canescence or slightly congested region of festering appear in the sick fish head portion of their early stage, trunk or fin, and when aggravation, lesions position skin rots completely, exposes muscular tissue, and sick fish can be dead because of septicemia; In addition, the fin ray of infected fish body also may be impaired, and pathogen, through being diffused into whole body herein, causes aggravation; Gill tissue is common infection position, and first usual gill filament end occurs that brown is downright bad, after progressively extend to base portion, the gill filament is rotten to the corn, occurs mud; The normal cluster of sick fish slowly moves about at the water surface, and body colour is turned black, and finally dead, usual fungus can invade disease fish diseased region as Secondary cases cause of disease, thus causes the state of an illness to be aggravated.This disease comes into vogue water temperature more than 20 DEG C, and the water temperature of cause of disease optimum breeding is 28 ~ 35 DEG C.Bacterial gill rot disease is comparatively large to the harm of Ietalurus Punetaus, and ill Ietalurus Punetaus mortality rate is higher.At present, the method that treatment Ietalurus Punetaus bacterial gill rot disease adopts usually adopts mass percentage concentration to be that the saline solution of 1%-3% embathes fish body, or adopt intramuscular injection gentamycin.But it is limited to adopt Sal to soak disease fish therapeutic effect, injection gentamicin treatment Ietalurus Punetaus not only complex operation, and often use gentamycin to cause abuse of antibiotics, be unfavorable for the sustainable health development of aquaculture industry.
Summary of the invention
An object of the present invention is to provide a kind of feed additive preventing and treating Ietalurus Punetaus bacterial gill rot disease, and its raw material components is green pure natural material, and easy to use, can the generation of effective prevention and therapy Ietalurus Punetaus bacterial gill rot disease.
In order to solve the problems of the technologies described above, the present invention is by the following technical solutions: a kind of feed additive preventing and treating Ietalurus Punetaus bacterial gill rot disease, be made up of each component of following masses number: Rhizoma Zingiberis Recens juice 35-45 part, Rhizoma Imperatae 26-32 part, Radix et Caulis Opuntiae Dillenii juice 26-32 part, Rhizoma Acori Graminei 25-33 part, Bulbus Allii Cepae juice 25-32 part, Herba Portulacae 22-30 part, Fructus Mume 22-28 part, Pericarpium Citri Reticulatae 22-28 part, Galla Chinensis 20-28 part, Semen Armeniacae Amarum powder 18-28 part, Herba Euphorbiae Helioscopiae 16-26 part, attached column flower 16-26 part, Herba Taraxaci 16-24 part, Rhizoma Belamcandae 14-24 part, Periostracum Cicadae 14-22 part, Cortex Hibisci 14-22 part, morning glory 12-20 part, Herba Aletridis Khasianae 12-18 part, Herba Saginae Japonicae 12-18 part, Radix Stephaniae Cepharanthae 13-17 part, Spica Prunellae 11-17 part, Bulbus Allii 9-17 part, Oleum menthae 8-16 part, Radix Alangii 7-13 part, Lumbricus powder 16-22 part, artemia powder 14-22 part, euphausia superba powder 14-22 part, Pulvis concha Mactrae 12-20 part, fish oil 10-18 part.
Preferably, the feed additive of described control Ietalurus Punetaus bacterial gill rot disease, be made up of each component of following masses number: Rhizoma Zingiberis Recens juice 40 parts, Rhizoma Imperatae 29 parts, Radix et Caulis Opuntiae Dillenii juice 29 parts, Rhizoma Acori Graminei 28 parts, Bulbus Allii Cepae juice 28 parts, Herba Portulacae 26 parts, Fructus Mume 26 parts, Pericarpium Citri Reticulatae 25 parts, Galla Chinensis 25 parts, Semen Armeniacae Amarum powder 23 parts, Herba Euphorbiae Helioscopiae 21 parts, attached column spends 21 parts, Herba Taraxaci 20 parts, Rhizoma Belamcandae 18 parts, Periostracum Cicadae 18 parts, Cortex Hibisci 18 parts, 16 parts, morning glory, Herba Aletridis Khasianae 15 parts, Herba Saginae Japonicae 15 parts, Radix Stephaniae Cepharanthae 15 parts, Spica Prunellae 14 parts, 14 parts, Bulbus Allii, Oleum menthae 12 parts, Radix Alangii 9 parts, Lumbricus powder 18 parts, artemia powder 19 parts, euphausia superba powder 20 parts, Pulvis concha Mactrae 17 parts, 14 parts, fish oil.
Preferably, the feed additive of described control Ietalurus Punetaus bacterial gill rot disease, be made up of each component of following masses number: Rhizoma Zingiberis Recens juice 40 parts, Rhizoma Imperatae 30 parts, Radix et Caulis Opuntiae Dillenii juice 30 parts, Rhizoma Acori Graminei 28 parts, Bulbus Allii Cepae juice 28 parts, Herba Portulacae 25 parts, Fructus Mume 25 parts, Pericarpium Citri Reticulatae 25 parts, Galla Chinensis 25 parts, Semen Armeniacae Amarum powder 22 parts, Herba Euphorbiae Helioscopiae 21 parts, attached column spends 21 parts, Herba Taraxaci 20 parts, Rhizoma Belamcandae 20 parts, Periostracum Cicadae 18 parts, Cortex Hibisci 18 parts, 15 parts, morning glory, Herba Aletridis Khasianae 14 parts, Herba Saginae Japonicae 14 parts, Radix Stephaniae Cepharanthae 14 parts, Spica Prunellae 14 parts, 12 parts, Bulbus Allii, Oleum menthae 12 parts, Radix Alangii 10 parts, Lumbricus powder 20 parts, artemia powder 20 parts, euphausia superba powder 18 parts, Pulvis concha Mactrae 15 parts, 14 parts, fish oil.
Each component effect of the present invention is resolved as follows:
Rhizoma Zingiberis Recens has bactericidal effect, and can strengthen digestion power.Rhizoma Imperatae is the rhizome of grass Herba Imperatae, can cooling blood for hemostasis, heat-clearing and toxic substances removing, can be used for spitting blood, hematuria, pyretic stranguria, edema, jaundice, dysuria, calentura excessive thirst, and gastric heat is vomitted the sound of vomiting etc.Radix et Caulis Opuntiae Dillenii sweet in the mouth is put down, can bactericidal antiphlogistic, can spleen reinforcing stomach invigorating, beneficial strength of one's legs, except chronic diarrhea, can also heat-clearing and toxic substances removing, and scattered silt is subsided a swelling, benefiting stomach and stopping pain, and antitussive is interior with controlling taste-blindness rate, acute dysentery, cough; Mumps is controlled in external, mastitis, carbuncle furuncle and phyma poison, venom, burn and scald.Rhizoma Acori Graminei can be dispelled epidemic disease Fructus Alpiniae Oxyphyllae, damp eliminating removing toxic substances, building body, the deinsectization of combustion Rhizoma Acori Graminei energy anophelifuge.Bulbus Allii Cepae nutritional labeling is very abundant, is not only rich in potassium, vitamin C, folic acid, zinc, selenium, and the nutrient such as cellulosic, more has the nutrient substance that two kinds special: Quercetin and prostaglandin A; Phytocide is contained as garlicin etc. in Bulbus Allii Cepae, there is very strong sterilizing ability, can effectively resist influenza virus, preventing cold, when the bacteriocidin of Bulbus Allii Cepae is discharged via respiratory tract, urinary tract, sweat gland, the cell duct wall of these positions can be stimulated to secrete, thus eliminate the phlegm again, diuresis, diaphoresis and the antibacterial effect such as anticorrosion.Herba Portulacae is Portulacaceae annual herb plant, herb hyoscine, has clearing away heat-damp and promoting diuresis, removing toxic substances and promoting subsidence of swelling, antiinflammatory, quenches the thirst, diuresis.Can enterokinesia be promoted containing boheic acid in Fructus Mume, in containing multiple organic acid, be improved the effect of liver function, therefore hepatopath should eat it, in addition, the prunus mume (sieb.) sieb.et zucc. acid in Fructus Mume can vessel softening, postpones sclerosis of blood vessels, has anti-senility effect.The circulation of qi promoting of Pericarpium Citri Reticulatae energy and in, stomach-QI being unable to descend normally, nausea and vomiting can be controlled, and can bactericidal.Galla Chinensis is cold in nature, sour in the mouth, puckery, has the effects such as pathogenic fire reducing of astringing the lung, relieving diarrhea with astringents, arresting sweating hemostasis, removing dampness sore, cures mainly the diseases such as chronic cough of deficiency lung, chronic diarrhea chronic dysentery, hemorrhoidal bleeding of having blood in stool, carbuncle sore tumefacting virus, Skin wet and rotten.Semen Armeniacae Amarum powder energy loosening bowel to relieve constipation, can treat pneumonopathy, can promote skin microcirculation, also has antitumor action.Herba Euphorbiae Helioscopiae acrid in the mouth, hardship, cold nature, line water detumescence, preventing phlegm from forming and stopping coughing, removing toxic substances parasite killing, cures mainly edematous asthma, and phlegm retention is breathed with cough malaria, bacillary dysentery, scrofula, Tuberculous fistula, osteomyelitis.Attached column flower mildly bitter flavor, pungent, cool in nature, can heat-clearing and toxic substances removing; Dampness removing is antipruritic; Reducing swelling and alleviating pain, cures mainly dysentery, skin infection, eczema, skin pruritus, caterpillar is stabbed, traumatic injury.Herba Taraxaci energy bactericidal antiphlogistic, heat-clearing and toxic substances removing, diuresis eliminating stagnation, controls acute mastitis, lymphadenitis, scrofula, furuncle carbuncle toxin, acute conjunctivitis, cold, fever, acute tonsillitis, acute bronchitis, gastritis, hepatitis, cholecystitis, urinary tract infection.Rhizoma Belamcandae is perennial herb, bitter in the mouth, cold in nature, micro-poison, and energy heat-clearing and toxic substances removing, eliminating stagnation antiinflammatory, reducing swelling and alleviating pain, relieving cough and resolving phlegm, be used for the treatment of tonsillitis and lumbago.Periostracum Cicadae energy dispelling wind-heat, rash sore-throat relieving, removing nebula to improve visual acuity, to expelling wind and relieving convulsion.Cortex Hibisci energy clearing away heat-damp and promoting diuresis, killing parasites for relieving itching, can be used for treating dysentery, leucorrhea; Eczema scrotum tinea corporis is controlled in external, tinea pedis.Morning glory has removing water retention by purgation diuresis, effect by expectorant, detumescence, parasite killing anthelmintic, cures mainly the diseases such as limds edema, oedema due to nephritis, cirrhotic ascites, constipation, abdominal pain due to worm stagnation.Herba Aletridis Khasianae is perennial herb, height about 30 centimetres, its acrid in the mouth; Micro-hardship; Warm in nature, can tonify deficiency arresting sweating; Hemostasis, the empty hyperhidrosis of main body; Night sweat; Neurasthenia; Spit blood; Have blood in stool.Herba Saginae Japonicae can be used for treating dermatitis rhus, tinea alba, carbuncle, scrofula, dental caries, children's's infantile dyspepsia, internal traumatic injury.Radix Stephaniae Cepharanthae is Menispermaceae stephania plant, is used as medicine with tuber, can heat-clearing and toxic substances removing, cooling blood for hemostasis, and dissipating blood stasis for subsidence of swelling, can be used for acute hepatitis, bacillary dysentery, acute appendicitis, stomachache, internal hemorrhage, traumatic injury, venom; Mumps is controlled in external, lymphadenitis, neurodermatitis.The decoct of Spica Prunellae has obvious antiinflammatory action; The decoct of Spica Prunellae all has certain inhibitory action to dysentery bacterium, Bacillus typhi, vibrio cholera, escherichia coli, Bacillus proteus, staphylococcus and Bacillus tuberculosis in vitro.Bulbus Allii removing toxic substances parasite killing, reducing swelling and alleviating pain, antidiarrheal dysentery relieving, controls lung, anthelmintic.Oleum menthae has the merit declaring malicious rash, can be used for controlling wind heat bundle table, measles without adequate eruption; Also can treat rubella pruritus, get the effect that its rash of dispeling the wind is antipruritic.Radix Alangii acrid in the mouth, hardship, warm in nature, poisonous, can expelling wind and removing dampness, relaxing muscles and tendons and activating QI and blood in the collateral, eliminating stasis to stop pain.Lumbricus is nutritious, and has food calling effect to Ietalurus Punetaus.Artemia contains rich in protein, and unsaturated fatty acid content is high, is the living body bait of Fish the best.Euphausia superba powder is not only nutritious; and the astaxanthin contained in euphausia superba powder serves fundamental role at the active oxygen species apoplexy due to endogenous wind of protection Fish; the beta-carotene contained in euphausia superba powder and astaxanthin play an important role in the immune system regulating Fish, can improve the immunity of Ietalurus Punetaus.Carnis Mactrae delicious meat, containing Multiple components such as protein, fat, carbohydrate, ferrum, calcium, phosphorus, iodine, vitamin, aminoacid and taurines, joins in the feedstuff of Ietalurus Punetaus and can also improve feedstuff mouthfeel.Fish oil can not only provide energy for Ietalurus Punetaus, and has the effect of food calling.
Another object of the present invention is to provide a kind of preparation method of feed additive of above-mentioned control Ietalurus Punetaus bacterial gill rot disease, and the preparation method of the feed additive of this control Ietalurus Punetaus bacterial gill rot disease comprises the following steps:
(1) take Rhizoma Imperatae and Rhizoma Acori Graminei according to proportioning, Rhizoma Imperatae and Rhizoma Acori Graminei are cleaned, drain the water, add the purified water of 4 times that parts by weight are Rhizoma Imperatae and Rhizoma Acori Graminei parts by weight, 70-80 DEG C is soaked 4-6h, filters to obtain infusion;
(2) take Herba Portulacae, Fructus Mume, Pericarpium Citri Reticulatae, Galla Chinensis, Herba Euphorbiae Helioscopiae, attached column flower, Herba Taraxaci, Cortex Hibisci, morning glory and Bulbus Allii according to proportioning, gross weight is designated as 1 part, adds 6 parts of purified water, and 20-30 DEG C is soaked 6-9h, obtain soak;
(3) boil 2h after the soak that step (2) obtains being heated to boiling, be separated and take out fried liquid, continue the purified water adding 3 parts in medical material, continue to boil 1h after being heated to boiling, merge twice fried liquid and be extracting solution;
(4) take Semen Armeniacae Amarum powder, Rhizoma Belamcandae, Periostracum Cicadae, Herba Aletridis Khasianae, Herba Saginae Japonicae, Radix Stephaniae Cepharanthae, Spica Prunellae and Radix Alangii respectively according to proportioning, load ball mill grinding 2h, cross 80 mesh sieves, obtain grounds travel;
(5) grounds travel that extracting solution step (3) obtained and step (4) obtain is positioned in the extraction kettle in supercritical extraction instrument, carbon dioxide is utilized to extract as medium, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure 12Mpa, separator temperature is 70 DEG C, extracts 2 hours, is extracted liquid;
(6) Lumbricus powder, artemia powder, euphausia superba powder and the Pulvis concha Mactrae that crush are crossed 50 mesh sieves respectively;
(7) take Rhizoma Zingiberis Recens juice, Radix et Caulis Opuntiae Dillenii juice, Bulbus Allii Cepae juice, Oleum menthae, Lumbricus powder, artemia powder, euphausia superba powder, Pulvis concha Mactrae and fish oil respectively according to proportioning, mix homogeneously with the extract of step (5) gained, obtain object.
The preparation method of described Rhizoma Zingiberis Recens juice is: Rhizoma Zingiberis Recens is put into juice extractor and squeezes the juice, and obtains Rhizoma Zingiberis Recens juice.
The preparation method of described Radix et Caulis Opuntiae Dillenii juice is: Radix et Caulis Opuntiae Dillenii is put into juice extractor and squeezes the juice, and obtains Radix et Caulis Opuntiae Dillenii juice.
The preparation method of described Bulbus Allii Cepae juice is: Bulbus Allii Cepae is put into juice extractor and squeezes the juice, and obtains Bulbus Allii Cepae juice.
The preparation method of described Lumbricus powder is: cleaned up by Lumbricus, pulverizes after putting into freezer dryer finish-drying, crosses 50 mesh sieves, obtains Lumbricus powder.Fully to retain the active component in Lumbricus body.
Usage and dosage: throw something and feed after the feed additive of control Ietalurus Punetaus bacterial gill rot disease of the present invention is mixed homogeneously with the weight ratio of 1:30-1:10 with commercially available normal feedstuff, preferably, the feed additive of control Ietalurus Punetaus bacterial gill rot disease of the present invention and the weight ratio of commercially available normal feedstuff are 1:20.
In preparation method, be that the effective ingredient of Rhizoma Imperatae and Rhizoma Acori Graminei is fully separated out by Rhizoma Imperatae and Rhizoma Acori Graminei in the object that 70-80 DEG C is soaked 4-6h, by Herba Portulacae, Fructus Mume, Pericarpium Citri Reticulatae, Galla Chinensis, Herba Euphorbiae Helioscopiae, attached column flower, Herba Taraxaci, Cortex Hibisci, morning glory and Bulbus Allii purified water soaking a period of time, medical material deliquescing, cell expansion, effective ingredient is easily fried, and medical material contains starch, protein, after soaking, prevent when decocting, the starch gelatinization on medical material surface, protein coagulating, the capillary channel on blocking medical material surface, the moisture when decocting is made to enter not go, effective ingredient is not easily fried, affect curative effect of medication, decoction can change the irritation effect of medicine, relatively mildly act on Ietalurus Punetaus, promote that Ietalurus Punetaus is to the absorption of medicine, by Semen Armeniacae Amarum powder, Rhizoma Belamcandae, Periostracum Cicadae, Herba Aletridis Khasianae, Herba Saginae Japonicae, Radix Stephaniae Cepharanthae, Spica Prunellae with Radix Alangii grinding can make the starch in medical material, protein separates out more fully at subsequent step, supercritical extraction, immersion, concentration step retain the effective ingredient of each component in the present invention to greatest extent, Rhizoma Zingiberis Recens, Radix et Caulis Opuntiae Dillenii, Bulbus Allii Cepae are put into juice extractor and squeeze the juice, the advantage of getting gained juice is that preparation method is simple, saves medicinal raw material, improves the utilization rate to Rhizoma Zingiberis Recens, Radix et Caulis Opuntiae Dillenii, Bulbus Allii Cepae, and take full advantage of the effective ingredient of Rhizoma Zingiberis Recens, Radix et Caulis Opuntiae Dillenii, Bulbus Allii Cepae, the better effects if of bactericidal.
Because the fish oil that contains in feed additive of the present invention and Lumbricus powder have food calling effect, Lumbricus powder, artemia powder, euphausia superba powder and Pulvis concha Mactrae nutritious, delicious flavour, artemia powder, euphausia superba powder and Pulvis concha Mactrae can also improve the overall mouthfeel of feedstuff, euphausia superba powder can also improve the immunity of Ietalurus Punetaus, therefore feed additive of the present invention can improve the mouthfeel of normal feedstuff, stimulate the taste bud of Ietalurus Punetaus, induction Ietalurus Punetaus is ingested and is added the normal feedstuff of feed additive of the present invention, therefore Ietalurus Punetaus immunity and resistance can be strengthened in feed additive of the present invention, the composition pathogen of bacterial gill rot disease to remarkable bactericidal and bacteriostatic effect can enter in Ietalurus Punetaus body, and play it and act on accordingly.
The feed additive of control Ietalurus Punetaus bacterial gill rot disease of the present invention is the etiopathogenesis according to bacterial gill rot disease, the suitable component of selection, and is organically combined, and has significant cooperative synergism effect, plays the function of each component to greatest extent.The component of the feed additive of control Ietalurus Punetaus bacterial gill rot disease of the present invention is environmental protection raw material, nontoxic to environment, not containing chemical drugs or antibiotic, is conducive to the sustainable health development of culture fishery; Can not affect the healthy of people after edible Ietalurus Punetaus of having cured, and feed additive of the present invention is good to the curative effect of Ietalurus Punetaus bacterial gill rot disease, cure rate is high, makes the survival rate of ill Ietalurus Punetaus to more than 90%.In addition; the present invention uses safer; have no side effect to Ietalurus Punetaus, after using the feed additive for the treatment of Ietalurus Punetaus bacterial gill rot disease of the present invention, Ietalurus Punetaus Health restoration is fast; after 3-8 days; Ietalurus Punetaus can recover normal activity, and idle fund is strong and vigorous, and wound progressively heals; the gill filament recovers normal, ingests completely normal.In addition, the feed additive of control Ietalurus Punetaus bacterial gill rot disease of the present invention effectively can also prevent the generation of Ietalurus Punetaus bacterial gill rot disease.
Compared with prior art, the invention has the beneficial effects as follows:
(1), in each component of the present invention, Rhizoma Zingiberis Recens has bactericidal effect, and can strengthen digestion power.Rhizoma Imperatae is the rhizome of grass Herba Imperatae, can cooling blood for hemostasis, heat-clearing and toxic substances removing, can be used for spitting blood, hematuria, pyretic stranguria, edema, jaundice, dysuria, calentura excessive thirst, and gastric heat is vomitted the sound of vomiting etc.Radix et Caulis Opuntiae Dillenii sweet in the mouth is put down, can bactericidal antiphlogistic, can spleen reinforcing stomach invigorating, beneficial strength of one's legs, except chronic diarrhea, can also heat-clearing and toxic substances removing, and scattered silt is subsided a swelling, benefiting stomach and stopping pain, and antitussive is interior with controlling taste-blindness rate, acute dysentery, cough; Mumps is controlled in external, mastitis, carbuncle furuncle and phyma poison, venom, burn and scald.Rhizoma Acori Graminei can be dispelled epidemic disease Fructus Alpiniae Oxyphyllae, damp eliminating removing toxic substances, building body, the deinsectization of combustion Rhizoma Acori Graminei energy anophelifuge.Bulbus Allii Cepae nutritional labeling is very abundant, is not only rich in potassium, vitamin C, folic acid, zinc, selenium, and the nutrient such as cellulosic, more has the nutrient substance that two kinds special: Quercetin and prostaglandin A; Phytocide is contained as garlicin etc. in Bulbus Allii Cepae, there is very strong sterilizing ability, can effectively resist influenza virus, preventing cold, when the bacteriocidin of Bulbus Allii Cepae is discharged via respiratory tract, urinary tract, sweat gland, the cell duct wall of these positions can be stimulated to secrete, thus eliminate the phlegm again, diuresis, diaphoresis and the antibacterial effect such as anticorrosion.Herba Portulacae is Portulacaceae annual herb plant, herb hyoscine, has clearing away heat-damp and promoting diuresis, removing toxic substances and promoting subsidence of swelling, antiinflammatory, quenches the thirst, diuresis.Can enterokinesia be promoted containing boheic acid in Fructus Mume, in containing multiple organic acid, be improved the effect of liver function, therefore hepatopath should eat it, in addition, the prunus mume (sieb.) sieb.et zucc. acid in Fructus Mume can vessel softening, postpones sclerosis of blood vessels, has anti-senility effect.The circulation of qi promoting of Pericarpium Citri Reticulatae energy and in, stomach-QI being unable to descend normally, nausea and vomiting can be controlled, and can bactericidal.Galla Chinensis is cold in nature, sour in the mouth, puckery, has the effects such as pathogenic fire reducing of astringing the lung, relieving diarrhea with astringents, arresting sweating hemostasis, removing dampness sore, cures mainly the diseases such as chronic cough of deficiency lung, chronic diarrhea chronic dysentery, hemorrhoidal bleeding of having blood in stool, carbuncle sore tumefacting virus, Skin wet and rotten.Semen Armeniacae Amarum powder energy loosening bowel to relieve constipation, can treat pneumonopathy, can promote skin microcirculation, also has antitumor action.Herba Euphorbiae Helioscopiae acrid in the mouth, hardship, cold nature, line water detumescence, preventing phlegm from forming and stopping coughing, removing toxic substances parasite killing, cures mainly edematous asthma, and phlegm retention is breathed with cough malaria, bacillary dysentery, scrofula, Tuberculous fistula, osteomyelitis.Attached column flower mildly bitter flavor, pungent, cool in nature, can heat-clearing and toxic substances removing; Dampness removing is antipruritic; Reducing swelling and alleviating pain, cures mainly dysentery, skin infection, eczema, skin pruritus, caterpillar is stabbed, traumatic injury.Herba Taraxaci energy bactericidal antiphlogistic, heat-clearing and toxic substances removing, diuresis eliminating stagnation, controls acute mastitis, lymphadenitis, scrofula, furuncle carbuncle toxin, acute conjunctivitis, cold, fever, acute tonsillitis, acute bronchitis, gastritis, hepatitis, cholecystitis, urinary tract infection.Rhizoma Belamcandae is perennial herb, bitter in the mouth, cold in nature, micro-poison, and energy heat-clearing and toxic substances removing, eliminating stagnation antiinflammatory, reducing swelling and alleviating pain, relieving cough and resolving phlegm, be used for the treatment of tonsillitis and lumbago.Periostracum Cicadae energy dispelling wind-heat, rash sore-throat relieving, removing nebula to improve visual acuity, to expelling wind and relieving convulsion.Cortex Hibisci energy clearing away heat-damp and promoting diuresis, killing parasites for relieving itching, can be used for treating dysentery, leucorrhea; Eczema scrotum tinea corporis is controlled in external, tinea pedis.Morning glory has removing water retention by purgation diuresis, effect by expectorant, detumescence, parasite killing anthelmintic, cures mainly the diseases such as limds edema, oedema due to nephritis, cirrhotic ascites, constipation, abdominal pain due to worm stagnation.Herba Aletridis Khasianae is perennial herb, height about 30 centimetres, its acrid in the mouth; Micro-hardship; Warm in nature, can tonify deficiency arresting sweating; Hemostasis, the empty hyperhidrosis of main body; Night sweat; Neurasthenia; Spit blood; Have blood in stool.Herba Saginae Japonicae can be used for treating dermatitis rhus, tinea alba, carbuncle, scrofula, dental caries, children's's infantile dyspepsia, internal traumatic injury.Radix Stephaniae Cepharanthae is Menispermaceae stephania plant, is used as medicine with tuber, can heat-clearing and toxic substances removing, cooling blood for hemostasis, and dissipating blood stasis for subsidence of swelling, can be used for acute hepatitis, bacillary dysentery, acute appendicitis, stomachache, internal hemorrhage, traumatic injury, venom; Mumps is controlled in external, lymphadenitis, neurodermatitis.The decoct of Spica Prunellae has obvious antiinflammatory action; The decoct of Spica Prunellae all has certain inhibitory action to dysentery bacterium, Bacillus typhi, vibrio cholera, escherichia coli, Bacillus proteus, staphylococcus and Bacillus tuberculosis in vitro.Bulbus Allii removing toxic substances parasite killing, reducing swelling and alleviating pain, antidiarrheal dysentery relieving, controls lung, anthelmintic.Oleum menthae has the merit declaring malicious rash, can be used for controlling wind heat bundle table, measles without adequate eruption; Also can treat rubella pruritus, get the effect that its rash of dispeling the wind is antipruritic.Radix Alangii acrid in the mouth, hardship, warm in nature, poisonous, can expelling wind and removing dampness, relaxing muscles and tendons and activating QI and blood in the collateral, eliminating stasis to stop pain.Lumbricus is nutritious, and has food calling effect to Ietalurus Punetaus.Artemia contains rich in protein, and unsaturated fatty acid content is high, is the living body bait of Fish the best.Euphausia superba powder is not only nutritious; and the astaxanthin contained in euphausia superba powder serves fundamental role at the active oxygen species apoplexy due to endogenous wind of protection Fish; the beta-carotene contained in euphausia superba powder and astaxanthin play an important role in the immune system regulating Fish, can improve the immunity of Ietalurus Punetaus.Carnis Mactrae delicious meat, containing Multiple components such as protein, fat, carbohydrate, ferrum, calcium, phosphorus, iodine, vitamin, aminoacid and taurines, joins in the feedstuff of Ietalurus Punetaus and can also improve feedstuff mouthfeel.Fish oil can not only provide energy for Ietalurus Punetaus, and has the effect of food calling.
(2) because the fish oil that contains in feed additive of the present invention and Lumbricus powder have food calling effect, Lumbricus powder, artemia powder, euphausia superba powder and Pulvis concha Mactrae nutritious, delicious flavour, artemia powder, euphausia superba powder and Pulvis concha Mactrae can also improve the overall mouthfeel of feedstuff, euphausia superba powder can also improve the immunity of Ietalurus Punetaus, therefore feed additive of the present invention can improve the mouthfeel of normal feedstuff, stimulate the taste bud of Ietalurus Punetaus, induction Ietalurus Punetaus is ingested and is added the normal feedstuff of feed additive of the present invention, therefore Ietalurus Punetaus immunity and resistance can be strengthened in feed additive of the present invention, the composition pathogen of bacterial gill rot disease to remarkable bactericidal and bacteriostatic effect can enter in Ietalurus Punetaus body, and play it and act on accordingly.
(3) feed additive of control Ietalurus Punetaus bacterial gill rot disease of the present invention, it is the Chinese herbal medicine selected according to the etiopathogenesis of bacterial gill rot disease, and organically combined, there is significant cooperative synergism effect, played the function of each component to greatest extent.
(4) pathogen of feed additive to Ietalurus Punetaus bacterial gill rot disease of control Ietalurus Punetaus bacterial gill rot disease of the present invention has good inhibition.
(5) component of the feed additive of control Ietalurus Punetaus bacterial gill rot disease of the present invention is environmental protection raw material, nontoxic to environment, without chemical drugs or antibiotic, is conducive to the sustainable health development of culture fishery; The healthy of people can not be affected after edible Ietalurus Punetaus of having cured, and the feed additive of control Ietalurus Punetaus bacterial gill rot disease of the present invention is very little to Ietalurus Punetaus damage, after using the feed additive of control Ietalurus Punetaus bacterial gill rot disease of the present invention, Ietalurus Punetaus Health restoration is fast.In addition, the feed additive of control Ietalurus Punetaus bacterial gill rot disease of the present invention is easy to use, will not pull rear medicine-feeding out by sick fish, decrease the injury to sick fish in operating process.
(6), in preparation method, be that the effective ingredient of Rhizoma Imperatae and Rhizoma Acori Graminei is fully separated out by Rhizoma Imperatae and Rhizoma Acori Graminei in the object that 70-80 DEG C is soaked 4-6h, by Herba Portulacae, Fructus Mume, Pericarpium Citri Reticulatae, Galla Chinensis, Herba Euphorbiae Helioscopiae, attached column flower, Herba Taraxaci, Cortex Hibisci, morning glory and Bulbus Allii purified water soaking a period of time, medical material deliquescing, cell expansion, effective ingredient is easily fried, and medical material contains starch, protein, after soaking, prevent when decocting, the starch gelatinization on medical material surface, protein coagulating, the capillary channel on blocking medical material surface, the moisture when decocting is made to enter not go, effective ingredient is not easily fried, affect curative effect of medication, decoction can change the irritation effect of medicine, relatively mildly act on Ietalurus Punetaus, promote that Ietalurus Punetaus is to the absorption of medicine.
(7) in preparation method, by Semen Armeniacae Amarum powder, Rhizoma Belamcandae, Periostracum Cicadae, Herba Aletridis Khasianae, Herba Saginae Japonicae, Radix Stephaniae Cepharanthae, Spica Prunellae with Radix Alangii grinding can make the starch in medical material, protein separates out more fully at subsequent step; Supercritical extraction, immersion, concentration step retain the effective ingredient of each component in the present invention to greatest extent.
(8) in preparation method, Rhizoma Zingiberis Recens, Radix et Caulis Opuntiae Dillenii, Bulbus Allii Cepae are put into juice extractor and squeeze the juice, the advantage of getting gained juice is that preparation method is simple, save medicinal raw material, improve the utilization rate to Rhizoma Zingiberis Recens, Radix et Caulis Opuntiae Dillenii, Bulbus Allii Cepae, and take full advantage of the effective ingredient of Rhizoma Zingiberis Recens, Radix et Caulis Opuntiae Dillenii, Bulbus Allii Cepae, the better effects if of bactericidal.
Detailed description of the invention
Following embodiment is convenient to understand the present invention better, but does not limit the present invention.Experimental technique in following embodiment, if no special instructions, is conventional method.
Embodiment 1
A kind of feed additive preventing and treating Ietalurus Punetaus bacterial gill rot disease, be made up of each component of following masses number: Rhizoma Zingiberis Recens juice 35 parts, Rhizoma Imperatae 26 parts, Radix et Caulis Opuntiae Dillenii juice 26 parts, Rhizoma Acori Graminei 25 parts, Bulbus Allii Cepae juice 25 parts, Herba Portulacae 24 parts, Fructus Mume 24 parts, Pericarpium Citri Reticulatae 22 parts, Galla Chinensis 20 parts, Semen Armeniacae Amarum powder 20 parts, Herba Euphorbiae Helioscopiae 18 parts, attached column spends 18 parts, Herba Taraxaci 18 parts, Rhizoma Belamcandae 16 parts, Periostracum Cicadae 16 parts, Cortex Hibisci 14 parts, 14 parts, morning glory, Herba Aletridis Khasianae 12 parts, Herba Saginae Japonicae 12 parts, Radix Stephaniae Cepharanthae 13 parts, Spica Prunellae 12 parts, 10 parts, Bulbus Allii, Oleum menthae 10 parts, Radix Alangii 9 parts, Lumbricus powder 16 parts, artemia powder 16 parts, euphausia superba powder 14 parts, Pulvis concha Mactrae 14 parts, 12 parts, fish oil.
The preparation method of the feed additive of above-mentioned control Ietalurus Punetaus bacterial gill rot disease comprises the following steps:
(1) take Rhizoma Imperatae and Rhizoma Acori Graminei according to proportioning, Rhizoma Imperatae and Rhizoma Acori Graminei are cleaned, drain the water, add the purified water of 4 times that parts by weight are Rhizoma Imperatae and Rhizoma Acori Graminei parts by weight, 70-80 DEG C is soaked 4-6h, filters to obtain infusion;
(2) take Herba Portulacae, Fructus Mume, Pericarpium Citri Reticulatae, Galla Chinensis, Herba Euphorbiae Helioscopiae, attached column flower, Herba Taraxaci, Cortex Hibisci, morning glory and Bulbus Allii according to proportioning, gross weight is designated as 1 part, adds 6 parts of purified water, and 20-30 DEG C is soaked 6-9h, obtain soak;
(3) boil 2h after the soak that step (2) obtains being heated to boiling, be separated and take out fried liquid, continue the purified water adding 3 parts in medical material, continue to boil 1h after being heated to boiling, merge twice fried liquid and be extracting solution;
(4) take Semen Armeniacae Amarum powder, Rhizoma Belamcandae, Periostracum Cicadae, Herba Aletridis Khasianae, Herba Saginae Japonicae, Radix Stephaniae Cepharanthae, Spica Prunellae and Radix Alangii respectively according to proportioning, load ball mill grinding 2h, cross 80 mesh sieves, obtain grounds travel;
(5) grounds travel that extracting solution step (3) obtained and step (4) obtain is positioned in the extraction kettle in supercritical extraction instrument, carbon dioxide is utilized to extract as medium, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure 12Mpa, separator temperature is 70 DEG C, extracts 2 hours, is extracted liquid;
(6) Lumbricus powder, artemia powder, euphausia superba powder and the Pulvis concha Mactrae that crush are crossed 50 mesh sieves respectively;
(7) take Rhizoma Zingiberis Recens juice, Radix et Caulis Opuntiae Dillenii juice, Bulbus Allii Cepae juice, Oleum menthae, Lumbricus powder, artemia powder, euphausia superba powder, Pulvis concha Mactrae and fish oil respectively according to proportioning, mix homogeneously with the extract of step (5) gained, obtain object.
The preparation method of described Rhizoma Zingiberis Recens juice is: Rhizoma Zingiberis Recens is put into juice extractor and squeezes the juice, and obtains Rhizoma Zingiberis Recens juice.
The preparation method of described Radix et Caulis Opuntiae Dillenii juice is: Radix et Caulis Opuntiae Dillenii is put into juice extractor and squeezes the juice, and obtains Radix et Caulis Opuntiae Dillenii juice.
The preparation method of described Bulbus Allii Cepae juice is: Bulbus Allii Cepae is put into juice extractor and squeezes the juice, and obtains Bulbus Allii Cepae juice.
The preparation method of described Lumbricus powder is: cleaned up by Lumbricus, pulverizes after putting into freezer dryer finish-drying, crosses 50 mesh sieves, obtains Lumbricus powder.
Embodiment 2
A kind of feed additive preventing and treating Ietalurus Punetaus bacterial gill rot disease, be made up of each component of following masses number: Rhizoma Zingiberis Recens juice 38 parts, Rhizoma Imperatae 28 parts, Radix et Caulis Opuntiae Dillenii juice 28 parts, Rhizoma Acori Graminei 25 parts, Bulbus Allii Cepae juice 25 parts, Herba Portulacae 22 parts, Fructus Mume 22 parts, Pericarpium Citri Reticulatae 24 parts, Galla Chinensis 22 parts, Semen Armeniacae Amarum powder 18 parts, Herba Euphorbiae Helioscopiae 16 parts, attached column spends 16 parts, Herba Taraxaci 16 parts, Rhizoma Belamcandae 14 parts, Periostracum Cicadae 14 parts, Cortex Hibisci 16 parts, 12 parts, morning glory, Herba Aletridis Khasianae 14 parts, Herba Saginae Japonicae 12 parts, Radix Stephaniae Cepharanthae 14 parts, Spica Prunellae 11 parts, 9 parts, Bulbus Allii, Oleum menthae 8 parts, Radix Alangii 7 parts, Lumbricus powder 18 parts, artemia powder 14 parts, euphausia superba powder 16 parts, Pulvis concha Mactrae 12 parts, 14 parts, fish oil.
The preparation method of the feed additive of the control Ietalurus Punetaus bacterial gill rot disease of the present embodiment is with embodiment 1.
Embodiment 3
A kind of feed additive preventing and treating Ietalurus Punetaus bacterial gill rot disease, be made up of each component of following masses number: Rhizoma Zingiberis Recens juice 45 parts, Rhizoma Imperatae 30 parts, Radix et Caulis Opuntiae Dillenii juice 32 parts, Rhizoma Acori Graminei 33 parts, Bulbus Allii Cepae juice 32 parts, Herba Portulacae 28 parts, Fructus Mume 28 parts, Pericarpium Citri Reticulatae 28 parts, Galla Chinensis 28 parts, Semen Armeniacae Amarum powder 26 parts, Herba Euphorbiae Helioscopiae 26 parts, attached column spends 26 parts, Herba Taraxaci 24 parts, Rhizoma Belamcandae 24 parts, Periostracum Cicadae 22 parts, Cortex Hibisci 22 parts, 20 parts, morning glory, Herba Aletridis Khasianae 18 parts, Herba Saginae Japonicae 18 parts, Radix Stephaniae Cepharanthae 16 parts, Spica Prunellae 16 parts, 17 parts, Bulbus Allii, Oleum menthae 16 parts, Radix Alangii 12 parts, Lumbricus powder 22 parts, artemia powder 22 parts, euphausia superba powder 22 parts, Pulvis concha Mactrae 20 parts, 10 parts, fish oil.
The preparation method of the feed additive of the control Ietalurus Punetaus bacterial gill rot disease of the present embodiment is with embodiment 1.
Embodiment 4
A kind of feed additive preventing and treating Ietalurus Punetaus bacterial gill rot disease, be made up of each component of following masses number: Rhizoma Zingiberis Recens juice 42 parts, Rhizoma Imperatae 32 parts, Radix et Caulis Opuntiae Dillenii juice 30 parts, Rhizoma Acori Graminei 31 parts, Bulbus Allii Cepae juice 30 parts, Herba Portulacae 30 parts, Fructus Mume 26 parts, Pericarpium Citri Reticulatae 28 parts, Galla Chinensis 26 parts, Semen Armeniacae Amarum powder 28 parts, Herba Euphorbiae Helioscopiae 24 parts, attached column spends 24 parts, Herba Taraxaci 24 parts, Rhizoma Belamcandae 22 parts, Periostracum Cicadae 20 parts, Cortex Hibisci 20 parts, 18 parts, morning glory, Herba Aletridis Khasianae 17 parts, Herba Saginae Japonicae 16 parts, Radix Stephaniae Cepharanthae 17 parts, Spica Prunellae 17 parts, 15 parts, Bulbus Allii, Oleum menthae 16 parts, Radix Alangii 13 parts, Lumbricus powder 22 parts, artemia powder 20 parts, euphausia superba powder 20 parts, Pulvis concha Mactrae 18 parts, 18 parts, fish oil.
The preparation method of the feed additive of the control Ietalurus Punetaus bacterial gill rot disease of the present embodiment is with embodiment 1.
Embodiment 5
A kind of feed additive preventing and treating Ietalurus Punetaus bacterial gill rot disease, be made up of each component of following masses number: Rhizoma Zingiberis Recens juice 40 parts, Rhizoma Imperatae 29 parts, Radix et Caulis Opuntiae Dillenii juice 29 parts, Rhizoma Acori Graminei 28 parts, Bulbus Allii Cepae juice 28 parts, Herba Portulacae 26 parts, Fructus Mume 26 parts, Pericarpium Citri Reticulatae 25 parts, Galla Chinensis 25 parts, Semen Armeniacae Amarum powder 23 parts, Herba Euphorbiae Helioscopiae 21 parts, attached column spends 21 parts, Herba Taraxaci 20 parts, Rhizoma Belamcandae 18 parts, Periostracum Cicadae 18 parts, Cortex Hibisci 18 parts, 16 parts, morning glory, Herba Aletridis Khasianae 15 parts, Herba Saginae Japonicae 15 parts, Radix Stephaniae Cepharanthae 15 parts, Spica Prunellae 14 parts, 14 parts, Bulbus Allii, Oleum menthae 12 parts, Radix Alangii 9 parts, Lumbricus powder 18 parts, artemia powder 19 parts, euphausia superba powder 20 parts, Pulvis concha Mactrae 17 parts, 14 parts, fish oil.
The preparation method of the feed additive of the control Ietalurus Punetaus bacterial gill rot disease of the present embodiment is with embodiment 1.
Embodiment 6
A kind of feed additive preventing and treating Ietalurus Punetaus bacterial gill rot disease, be made up of each component of following masses number: Rhizoma Zingiberis Recens juice 40 parts, Rhizoma Imperatae 30 parts, Radix et Caulis Opuntiae Dillenii juice 30 parts, Rhizoma Acori Graminei 28 parts, Bulbus Allii Cepae juice 28 parts, Herba Portulacae 25 parts, Fructus Mume 25 parts, Pericarpium Citri Reticulatae 25 parts, Galla Chinensis 25 parts, Semen Armeniacae Amarum powder 22 parts, Herba Euphorbiae Helioscopiae 21 parts, attached column spends 21 parts, Herba Taraxaci 20 parts, Rhizoma Belamcandae 20 parts, Periostracum Cicadae 18 parts, Cortex Hibisci 18 parts, 15 parts, morning glory, Herba Aletridis Khasianae 14 parts, Herba Saginae Japonicae 14 parts, Radix Stephaniae Cepharanthae 14 parts, Spica Prunellae 14 parts, 12 parts, Bulbus Allii, Oleum menthae 12 parts, Radix Alangii 10 parts, Lumbricus powder 20 parts, artemia powder 20 parts, euphausia superba powder 18 parts, Pulvis concha Mactrae 15 parts, 14 parts, fish oil.
The preparation method of the feed additive of the control Ietalurus Punetaus bacterial gill rot disease of the present embodiment is with embodiment 1.
Experimental result
Contrast experiment 1
Test site is selected in fishing ground, Qingdao, in order to verify therapeutic effect of the present invention, is testing the Ietalurus Punetaus suffering from bacterial gill rot disease:
The process of matched group is as follows: every day mass percentage concentration be 2% saline solution embathe ill fish body 15 minutes ~ 20 minutes, until sick fish recovery from illness.
The process of experiment A group is as follows: throw something and feed after being mixed homogeneously with the weight ratio of 1:20 with commercially available normal feedstuff by the feed additive of the control Ietalurus Punetaus bacterial gill rot disease of the embodiment of the present invention 1, until the recovery from illness of sick fish.
The process of experiment B group is as follows: throw something and feed after being mixed homogeneously with the weight ratio of 1:20 with commercially available normal feedstuff by the feed additive of the control Ietalurus Punetaus bacterial gill rot disease of the embodiment of the present invention 2, until the recovery from illness of sick fish.
The process of experiment C group is as follows: throw something and feed after being mixed homogeneously with the weight ratio of 1:20 with commercially available normal feedstuff by the feed additive of the control Ietalurus Punetaus bacterial gill rot disease of the embodiment of the present invention 3, until the recovery from illness of sick fish.
The process of experiment D group is as follows: throw something and feed after being mixed homogeneously with the weight ratio of 1:20 with commercially available normal feedstuff by the feed additive of the control Ietalurus Punetaus bacterial gill rot disease of the embodiment of the present invention 4, until the recovery from illness of sick fish.
The process of experiment E group is as follows: throw something and feed after being mixed homogeneously with the weight ratio of 1:20 with commercially available normal feedstuff by the feed additive of the control Ietalurus Punetaus bacterial gill rot disease of the embodiment of the present invention 5, until the recovery from illness of sick fish.
The process of experiment F group is as follows: throw something and feed after being mixed homogeneously with the weight ratio of 1:20 with commercially available normal feedstuff by the feed additive of the control Ietalurus Punetaus bacterial gill rot disease of the embodiment of the present invention 6, until the recovery from illness of sick fish.
Experimental result is in table 1:
The feed additive therapeutic effect of table 1 control Ietalurus Punetaus of the present invention bacterial gill rot disease
Experimental result shows: feed additive of the present invention is good to the curative effect of Ietalurus Punetaus bacterial gill rot disease; cure rate is high; make the survival rate of ill Ietalurus Punetaus to more than 90%; the present invention uses safer; Ietalurus Punetaus is had no side effect; after using the feed additive for the treatment of Ietalurus Punetaus bacterial gill rot disease of the present invention; Ietalurus Punetaus Health restoration is fast; medication 3-8 days; Ietalurus Punetaus can recover normal activity, and idle fund is strong and vigorous, and wound progressively heals; the gill filament recovers normal, ingests completely normal.
Contrast experiment 2
Normally to raise group for blank group.Experimental group process is as follows:
Experiment a group: throw something and feed after the feed additive of the control Ietalurus Punetaus bacterial gill rot disease of the embodiment of the present invention 1 is mixed homogeneously with the weight ratio of 1:30 with commercially available normal feedstuff.
Experiment b group: throw something and feed after the feed additive of the control Ietalurus Punetaus bacterial gill rot disease of the embodiment of the present invention 2 is mixed homogeneously with the weight ratio of 1:30 with commercially available normal feedstuff.
Experiment c group: throw something and feed after the feed additive of the control Ietalurus Punetaus bacterial gill rot disease of the embodiment of the present invention 3 is mixed homogeneously with the weight ratio of 1:30 with commercially available normal feedstuff.
Experiment d group: throw something and feed after the feed additive of the control Ietalurus Punetaus bacterial gill rot disease of the embodiment of the present invention 4 is mixed homogeneously with the weight ratio of 1:30 with commercially available normal feedstuff.
Experiment e group: throw something and feed after the feed additive of the control Ietalurus Punetaus bacterial gill rot disease of the embodiment of the present invention 5 is mixed homogeneously with the weight ratio of 1:30 with commercially available normal feedstuff.
Experiment f group: throw something and feed after the feed additive of the control Ietalurus Punetaus bacterial gill rot disease of the embodiment of the present invention 6 is mixed homogeneously with the weight ratio of 1:30 with commercially available normal feedstuff.
When experiment starts, each processed group puts into healthy Ietalurus Punetaus 10, after raising 20 days, adds the Ietalurus Punetaus that 1 is suffered from bacterial gill rot disease in each processed group by above-mentioned process, observes and records infection state after one week.The results are shown in Table 2:
The effect of the feed additive prevention bacterial gill rot disease of table 2 control Ietalurus Punetaus of the present invention bacterial gill rot disease
From table 2, the feed additive of control Ietalurus Punetaus bacterial gill rot disease of the present invention effectively can reduce the incidence rate of Ietalurus Punetaus bacterial gill rot disease, the immunity of Ietalurus Punetaus can be improved, to bacterial gill rot disease, there is good preventive effect.
Bacteriostatic test checks
With Sal as matched group, the feed additive of the control Ietalurus Punetaus bacterial gill rot disease of embodiment of the present invention 1-embodiment 6 is that experimental group carries out antibacterial inspection contrast.
Culture medium: 0.5% tryptone culture medium.
Strain is: flavobacterium columnare.
Matched group antibacterial liquid is prepared: being dissolved in by Sal in sterile deionized water and being diluted to mass percentage concentration is 2%.
Experimental group antibacterial liquid is prepared: be dissolved in respectively in sterile deionized water by the feed additive of the control Ietalurus Punetaus bacterial gill rot disease of embodiment of the present invention 1-embodiment 6, liquor strength being diluted to mass percentage concentration is 2%.
Observe and record experimental result:
Represent with "-" without inhibition zone;
Antibacterial circle diameter < 10mm is slightly antibacterial, represents with "+";
Antibacterial circle diameter 10 ~ 14mm is that moderate is antibacterial, represents with " ++ ";
Antibacterial circle diameter > 14mm is highly antibacterial, represents with " +++ "; To the fungistatic effect of flavobacterium columnare in table 3:
The bactericidal and bacteriostatic effect of the feed additive of table 3 control Ietalurus Punetaus of the present invention bacterial gill rot disease
From table 3, the pathogen flavobacterium columnare of feed additive to bacterial gill rot disease of control Ietalurus Punetaus bacterial gill rot disease of the present invention has good bactericidal and bacteriostatic effect.
The above is only the preferred embodiment of the present invention, is noted that for those skilled in the art, and under the premise without departing from the principles of the invention, can also make some improvement, these improvement should be considered as protection scope of the present invention.
Claims (10)
1. prevent and treat the feed additive of Ietalurus Punetaus bacterial gill rot disease for one kind, it is characterized in that: be made up of each component of following masses number: Rhizoma Zingiberis Recens juice 35-45 part, Rhizoma Imperatae 26-32 part, Radix et Caulis Opuntiae Dillenii juice 26-32 part, Rhizoma Acori Graminei 25-33 part, Bulbus Allii Cepae juice 25-32 part, Herba Portulacae 22-30 part, Fructus Mume 22-28 part, Pericarpium Citri Reticulatae 22-28 part, Galla Chinensis 20-28 part, Semen Armeniacae Amarum powder 18-28 part, Herba Euphorbiae Helioscopiae 16-26 part, attached column flower 16-26 part, Herba Taraxaci 16-24 part, Rhizoma Belamcandae 14-24 part, Periostracum Cicadae 14-22 part, Cortex Hibisci 14-22 part, morning glory 12-20 part, Herba Aletridis Khasianae 12-18 part, Herba Saginae Japonicae 12-18 part, Radix Stephaniae Cepharanthae 13-17 part, Spica Prunellae 11-17 part, Bulbus Allii 9-17 part, Oleum menthae 8-16 part, Radix Alangii 7-13 part, Lumbricus powder 16-22 part, artemia powder 14-22 part, euphausia superba powder 14-22 part, Pulvis concha Mactrae 12-20 part, fish oil 10-18 part.
2. the feed additive of control Ietalurus Punetaus bacterial gill rot disease according to claim 1, it is characterized in that: the feed additive of described control Ietalurus Punetaus bacterial gill rot disease, be made up of each component of following masses number: Rhizoma Zingiberis Recens juice 40 parts, Rhizoma Imperatae 29 parts, Radix et Caulis Opuntiae Dillenii juice 29 parts, Rhizoma Acori Graminei 28 parts, Bulbus Allii Cepae juice 28 parts, Herba Portulacae 26 parts, Fructus Mume 26 parts, Pericarpium Citri Reticulatae 25 parts, Galla Chinensis 25 parts, Semen Armeniacae Amarum powder 23 parts, Herba Euphorbiae Helioscopiae 21 parts, attached column spends 21 parts, Herba Taraxaci 20 parts, Rhizoma Belamcandae 18 parts, Periostracum Cicadae 18 parts, Cortex Hibisci 18 parts, 16 parts, morning glory, Herba Aletridis Khasianae 15 parts, Herba Saginae Japonicae 15 parts, Radix Stephaniae Cepharanthae 15 parts, Spica Prunellae 14 parts, 14 parts, Bulbus Allii, Oleum menthae 12 parts, Radix Alangii 9 parts, Lumbricus powder 18 parts, artemia powder 19 parts, euphausia superba powder 20 parts, Pulvis concha Mactrae 17 parts, 14 parts, fish oil.
3. the feed additive of control Ietalurus Punetaus bacterial gill rot disease according to claim 1, it is characterized in that: the feed additive of described control Ietalurus Punetaus bacterial gill rot disease, be made up of each component of following masses number: Rhizoma Zingiberis Recens juice 40 parts, Rhizoma Imperatae 30 parts, Radix et Caulis Opuntiae Dillenii juice 30 parts, Rhizoma Acori Graminei 28 parts, Bulbus Allii Cepae juice 28 parts, Herba Portulacae 25 parts, Fructus Mume 25 parts, Pericarpium Citri Reticulatae 25 parts, Galla Chinensis 25 parts, Semen Armeniacae Amarum powder 22 parts, Herba Euphorbiae Helioscopiae 21 parts, attached column spends 21 parts, Herba Taraxaci 20 parts, Rhizoma Belamcandae 20 parts, Periostracum Cicadae 18 parts, Cortex Hibisci 18 parts, 15 parts, morning glory, Herba Aletridis Khasianae 14 parts, Herba Saginae Japonicae 14 parts, Radix Stephaniae Cepharanthae 14 parts, Spica Prunellae 14 parts, 12 parts, Bulbus Allii, Oleum menthae 12 parts, Radix Alangii 10 parts, Lumbricus powder 20 parts, artemia powder 20 parts, euphausia superba powder 18 parts, Pulvis concha Mactrae 15 parts, 14 parts, fish oil.
4. a preparation method for the feed additive of the control Ietalurus Punetaus bacterial gill rot disease according to any one of claim 1-3, is characterized in that: comprise the following steps:
(1) take Rhizoma Imperatae and Rhizoma Acori Graminei according to proportioning, Rhizoma Imperatae and Rhizoma Acori Graminei are cleaned, drain the water, add the purified water of 4 times that parts by weight are Rhizoma Imperatae and Rhizoma Acori Graminei parts by weight, 70-80 DEG C is soaked 4-6h, filters to obtain infusion;
(2) take Herba Portulacae, Fructus Mume, Pericarpium Citri Reticulatae, Galla Chinensis, Herba Euphorbiae Helioscopiae, attached column flower, Herba Taraxaci, Cortex Hibisci, morning glory and Bulbus Allii according to proportioning, gross weight is designated as 1 part, adds 6 parts of purified water, and 20-30 DEG C is soaked 6-9h, obtain soak;
(3) boil 2h after the soak that step (2) obtains being heated to boiling, be separated and take out fried liquid, continue the purified water adding 3 parts in medical material, continue to boil 1h after being heated to boiling, merge twice fried liquid and be extracting solution;
(4) take Semen Armeniacae Amarum powder, Rhizoma Belamcandae, Periostracum Cicadae, Herba Aletridis Khasianae, Herba Saginae Japonicae, Radix Stephaniae Cepharanthae, Spica Prunellae and Radix Alangii respectively according to proportioning, load ball mill grinding 2h, cross 80 mesh sieves, obtain grounds travel;
(5) grounds travel that extracting solution step (3) obtained and step (4) obtain is positioned in the extraction kettle in supercritical extraction instrument, carbon dioxide is utilized to extract as medium, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure 12Mpa, separator temperature is 70 DEG C, extracts 2 hours, is extracted liquid;
(6) Lumbricus powder, artemia powder, euphausia superba powder and the Pulvis concha Mactrae that crush are crossed 50 mesh sieves respectively;
(7) take Rhizoma Zingiberis Recens juice, Radix et Caulis Opuntiae Dillenii juice, Bulbus Allii Cepae juice, Oleum menthae, Lumbricus powder, artemia powder, euphausia superba powder, Pulvis concha Mactrae and fish oil respectively according to proportioning, mix homogeneously with the extract of step (5) gained, obtain object.
5. the preparation method of the feed additive of control Ietalurus Punetaus bacterial gill rot disease according to claim 4, is characterized in that: the preparation method of described Rhizoma Zingiberis Recens juice is: Rhizoma Zingiberis Recens is put into juice extractor and squeezes the juice, and obtains Rhizoma Zingiberis Recens juice.
6. the preparation method of the feed additive of control Ietalurus Punetaus bacterial gill rot disease according to claim 4, is characterized in that: the preparation method of described Radix et Caulis Opuntiae Dillenii juice is: Radix et Caulis Opuntiae Dillenii is put into juice extractor and squeezes the juice, and obtains Radix et Caulis Opuntiae Dillenii juice.
7. the preparation method of the feed additive of control Ietalurus Punetaus bacterial gill rot disease according to claim 4, is characterized in that: the preparation method of described Bulbus Allii Cepae juice is: Bulbus Allii Cepae is put into juice extractor and squeezes the juice, and obtains Bulbus Allii Cepae juice.
8. the preparation method of the feed additive of control Ietalurus Punetaus bacterial gill rot disease according to claim 4, it is characterized in that: the preparation method of described Lumbricus powder is: cleaned up by Lumbricus, pulverize after putting into freezer dryer finish-drying, cross 50 mesh sieves, obtain Lumbricus powder.
9. a using method for the feed additive of the control Ietalurus Punetaus bacterial gill rot disease according to any one of claim 1-3, is characterized in that: throw something and feed after being mixed homogeneously with the weight ratio of 1:30-1:10 with commercially available normal feedstuff by the feed additive of control Ietalurus Punetaus bacterial gill rot disease of the present invention.
10. the using method of the feed additive of control Ietalurus Punetaus bacterial gill rot disease according to claim 9, is characterized in that: the feed additive of control Ietalurus Punetaus bacterial gill rot disease of the present invention and the weight ratio of commercially available normal feedstuff are 1:20.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510845850.6A CN105327318A (en) | 2015-11-27 | 2015-11-27 | Feed additive for preventing and treating bacterial gill-rot disease of ictalurus punctatus and preparation method of feed additive |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510845850.6A CN105327318A (en) | 2015-11-27 | 2015-11-27 | Feed additive for preventing and treating bacterial gill-rot disease of ictalurus punctatus and preparation method of feed additive |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105327318A true CN105327318A (en) | 2016-02-17 |
Family
ID=55278224
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510845850.6A Pending CN105327318A (en) | 2015-11-27 | 2015-11-27 | Feed additive for preventing and treating bacterial gill-rot disease of ictalurus punctatus and preparation method of feed additive |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105327318A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105815605A (en) * | 2016-03-30 | 2016-08-03 | 青岛海之星生物科技有限公司 | Feed for preventing silurus lanzhouensis gill rot and preparation method thereof |
| CN106386582A (en) * | 2016-08-31 | 2017-02-15 | 南通华鎏水产有限公司 | Breeding method of Ietalurus punetaus |
| CN110393238A (en) * | 2019-07-24 | 2019-11-01 | 肇庆学院 | A kind of anti-fresh-water fishes flavobacterium columnare feed and preparation method thereof |
| CN114711345A (en) * | 2022-05-06 | 2022-07-08 | 通威股份有限公司 | Chinese herbal medicine additive for preventing and treating bacterial rot disease of alepidote fish and application thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101214362A (en) * | 2007-12-27 | 2008-07-09 | 李勇军 | Preparations for treating bacterial infection disease in fishes and preparation |
| CN103229908A (en) * | 2013-04-19 | 2013-08-07 | 中国水产科学研究院东海水产研究所 | Acipenser gueldenstaedtii feed prepared from euphausia supetba dana meal |
| CN103656401A (en) * | 2012-09-24 | 2014-03-26 | 天津瑞贝特科技发展有限公司 | Traditional Chinese medicine composition for preventing and treating bacterial gill-rot disease of freshwater fish and preparation method thereof |
| CN104758555A (en) * | 2015-04-24 | 2015-07-08 | 河北科技师范学院 | Chinese herbal medicine for curing marine fish bacterial diseases |
| CN105076834A (en) * | 2015-08-31 | 2015-11-25 | 青岛海之源智能技术有限公司 | Migration type adult Salmo salar mixed feed and preparation method thereof |
-
2015
- 2015-11-27 CN CN201510845850.6A patent/CN105327318A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101214362A (en) * | 2007-12-27 | 2008-07-09 | 李勇军 | Preparations for treating bacterial infection disease in fishes and preparation |
| CN103656401A (en) * | 2012-09-24 | 2014-03-26 | 天津瑞贝特科技发展有限公司 | Traditional Chinese medicine composition for preventing and treating bacterial gill-rot disease of freshwater fish and preparation method thereof |
| CN103229908A (en) * | 2013-04-19 | 2013-08-07 | 中国水产科学研究院东海水产研究所 | Acipenser gueldenstaedtii feed prepared from euphausia supetba dana meal |
| CN104758555A (en) * | 2015-04-24 | 2015-07-08 | 河北科技师范学院 | Chinese herbal medicine for curing marine fish bacterial diseases |
| CN105076834A (en) * | 2015-08-31 | 2015-11-25 | 青岛海之源智能技术有限公司 | Migration type adult Salmo salar mixed feed and preparation method thereof |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105815605A (en) * | 2016-03-30 | 2016-08-03 | 青岛海之星生物科技有限公司 | Feed for preventing silurus lanzhouensis gill rot and preparation method thereof |
| CN106386582A (en) * | 2016-08-31 | 2017-02-15 | 南通华鎏水产有限公司 | Breeding method of Ietalurus punetaus |
| CN110393238A (en) * | 2019-07-24 | 2019-11-01 | 肇庆学院 | A kind of anti-fresh-water fishes flavobacterium columnare feed and preparation method thereof |
| CN114711345A (en) * | 2022-05-06 | 2022-07-08 | 通威股份有限公司 | Chinese herbal medicine additive for preventing and treating bacterial rot disease of alepidote fish and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105166544A (en) | Pig mixed feed and preparation method thereof | |
| CN105327318A (en) | Feed additive for preventing and treating bacterial gill-rot disease of ictalurus punctatus and preparation method of feed additive | |
| CN105104757A (en) | Chinese herbal medicine feed additive for preventing ichthyophthiriasis of dragonfish and preparation method of Chinese herbal medicine feed additive | |
| CN103239651B (en) | Traditional Chinese medicine for treating subclinical mastitis for dairy cow | |
| CN104256166A (en) | Functional feed for treating bovine bluetongue and preparation method thereof | |
| CN105412670A (en) | Immunopotentiator for preventing and treating tilapia branchiomycosis and preparation method thereof | |
| CN105285523A (en) | Feed additive for preventing haemorrhagic septicaemia of ictalurus punctatus and preparation method of feed additive | |
| CN105194231A (en) | Chinese herb feed additive for preventing bacterial gill-rot disease of fancy carps and preparation method thereof | |
| CN105918703A (en) | Feed additive treating megalobrama amblycephala red skin diseases and preparation method thereof | |
| CN105106725A (en) | Swordtail bacillary enteritis preventing feed additive and preparation method thereof | |
| CN105169318A (en) | Traditional Chinese medicinal herbal preparation for treating ichthyophthiriasis of osteoglossum bicirrhosum | |
| CN105396110A (en) | Powder used for preventing branchiomycosis of tilapia and preparation method thereof | |
| CN103845535A (en) | Pharmaceutical composition for debriding and eliminating inflammation | |
| CN105394440A (en) | Feed additive for preventing gill-rot disease of lateolabrax japonicus and preparation method thereof | |
| CN105396097A (en) | Compound preparation capable of preventing and treating channel catfish bacterial gill-rot disease and preparation method of compound preparation | |
| CN105341534A (en) | Feed additive for preventing white-spot disease of parrot fishes and preparation method thereof | |
| CN104707008B (en) | A kind of composite drug and preparation method for preventing chickens respiratory disease | |
| CN105749224A (en) | Immunopotentiator for preventing and treating channel catfish ichthyophthiriasis and preparation method thereof | |
| CN105288302A (en) | Feed additive for preventing and treating fester disease of Epinephelus akaara and preparation method of feed additive | |
| CN105327319A (en) | Tincture for treating bacterial gill-rot disease of channel catfish and preparation method thereof | |
| CN107789525A (en) | It is a kind of to treat the medicine of ulcer on the shank ulcer for many years not more and preparation method thereof | |
| CN105410481A (en) | Feed additive for preventing and treating turbot fin rot and preparation method thereof | |
| CN108029878A (en) | A kind of feed addictive and its production method for preventing hog cholera | |
| CN107960535A (en) | A kind of feed addictive and its production method for preventing beef cattle gastroenteritis | |
| CN107296917A (en) | One kind preventing and treating wood frog red leg disease Chinese medicine composition |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160217 |
|
| RJ01 | Rejection of invention patent application after publication |