CN105325768A - Feed additive preventing scale protrusion disease of pterophyllum and preparation method thereof - Google Patents
Feed additive preventing scale protrusion disease of pterophyllum and preparation method thereof Download PDFInfo
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- CN105325768A CN105325768A CN201510724131.9A CN201510724131A CN105325768A CN 105325768 A CN105325768 A CN 105325768A CN 201510724131 A CN201510724131 A CN 201510724131A CN 105325768 A CN105325768 A CN 105325768A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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Abstract
The invention relates to a feed additive preventing the scale protrusion disease of pterophyllum and a preparation method thereof. The feed additive preventing the scale protrusion disease of pterophyllum is prepared from, by mass, 28-35 parts of cactus juice, 19-25 parts of oenanthe stolonifera, 19-23 parts of aqueous extracts of garden burnet, 10-18 parts of cooked puerh tea, 18-23 parts of morning-glory, 17-23 parts of Japonese pearlwort herbs, 16-22 parts of mazus japonicus, 15-21 parts of common cephalanoplos herbs, 14-20 parts of cocklebur fruits, 14-18 parts of selfheal, 12-19 parts of black nightshade, 12-18 parts of polygonum multiflorum, 12-18 parts of roots of bidentate achyranthes, 9-16 parts of rubus pluribracteatus roots, 9-16 parts of aletris lanuginosa bureau et Franch, 7-15 parts of nettlesand the like. The feed additive preventing the scale protrusion disease of pterophyllum can effectively prevent the scale protrusion disease of pterophyllum and improve the immunity of pterophyllum.
Description
Technical field
The invention belongs to technical field of aquaculture, particularly relate to a kind of feed addictive preventing angle fish scales upright; The invention still further relates to a kind of preparation method of feed addictive of above-mentioned prevention angle fish scales upright.
Background technology
Along with the growth in the living standard of people, material life is enriched greatly, and the requirement that people live to amusement and recreation is more and more obvious, and staying at home or supporting pet fish beautiful in colour a bit in garden is best selection, takes up room hardly because raise pet fish; Economic cost can be determined according to people; Condition is relatively simple, has water.Cultivation of ornamental fish not only can be cultivated the moral character and nourished the nature, and cultivates one's taste, and the anion discharged in water and steam also can filter and wet air, build good living environment to people.
Angle fish, has another name called Spanish mackerel, angel, fairyhood fish, finlet sail fish etc., and beautiful flying fish belongs to, and originates in Guyana of South America, Brazil.Long 12 ~ 15 centimetres of angle fish, Gao Keda 15 ~ 20 centimetres, a little and sharp side is flat, assumes diamond in shape.Dorsal fin and anal fin are grown up very much, tall and straight as jib, therefore have the title of finlet sail fish.Angle fish moves about from the side, and as swallow fly, therefore angle fish is also known as Spanish mackerel.
Scales upright is named again that squama is vertical sick, the pinecone is sick, this disease be fish body injured after be infected by bacterial, cause a kind of disease that scale follice inner accumulated liquid causes scale to be erect.Its pathogen is small-sized point-like pole hair bacillus.The symptom of this disease is: sick fish body surface is coarse, and body surface swelling causes scale to be holded up.It is many because frequently changing caused by water that angle fish suffers from this disease.Time ill, angle fish majority outwards opens as the pinecone at the part scale of afterbody, and the scale follice oedema of scale base portion, long-pending have translucent or containing the diffusate of blood on a small quantity, congested with epidermis, exophthalmos '.If pressure a little on scale, namely visible flakes base portion has a small amount of diffusate to spray, and then scale comes off.Time serious, scale is just as the hair of hedgehog, and blade is holded up, withered loose, abdominal distention, and the base portion of fin is congested, and fin has rotten phenomenon.Sick fish swimming is blunt, expiratory dyspnea, and health hangs upside down, and upwards, 2-3 days namely dead afterwards for belly.The morbidity scope of scales upright is wide, from fingerling to juvenile fish, all have generation.Angle fish scales upright is popular at the beginning of last month of spring in winter, is low temperature disease.The scales upright of current treatment angle fish there is no effective medicine, the means of usual employing are with the sick fish of isolation, then the treatment in 5-10 minute of fish body is embathed with 5ppm copper sulphate, 2ppm ferrous sulfate and 10ppm bleaching powder mixed liquor, its result for the treatment of is effective, by chemical substance, fish body sterilizing is easily easily polluted environment, and while treatment scales upright, larger damage be also result in angle fish.Therefore, that prevents scales upright has important production meaning, and the feed that develop a kind of environmental protection, angle fish scales upright effectively can be prevented to occur or feed addictive, have important economic implications and market prospects.
Summary of the invention
An object of the present invention is to provide a kind of feed addictive preventing angle fish scales upright, its raw material components is green pure natural material, there is good restraining and sterilizing bacteria effect to the pathogen of angle fish scales upright small-sized point-like pole hair bacillus, effectively can reduce the incidence of disease of angle fish scales upright, the immunity improving angle fish and survival rate.
In order to solve the problems of the technologies described above, the present invention by the following technical solutions, a kind of feed addictive preventing angle fish scales upright, be made up of each component of following masses number: cactus juice 28-35 part, oenanthe stolonifera 19-25 part, infusion 19-23 part of garden burnet, Pu'er cooked tea 10-18 part, morning glory 18-23 part, sealwort 17-23 part, Fructus Sophorae 17-22 part, Mazus japonicus 16-22 part, field thistle 15-21 part, achene of Siberian cocklebur 14-20 part, selfheal 14-18 part, black nightshade 12-19 part, fleece-flower root 12-18 part, root of bidentate achyranthes 12-18 part, rhizoma atractylodis 10-18 part, dandelion 10-16 part, szechwan raspberry root or leaf 9-16 part, khas aletris herb 9-16 part, ginger 8-14 part, nettle 7-15 part, bamboo salt 7-15 part, euphausia superba powder 13-19 part, dry earthworm powder 11-17 part, dry clam worm powder 11-17 part, fish oil 12-18 part, honey 12-18 part, soybean oil 8-14 part, the described fleece-flower root is identical with the mass fraction of the root of bidentate achyranthes, described szechwan raspberry root or leaf is identical with the mass fraction of khas aletris herb.
Preferably, the feed addictive of described prevention angle fish scales upright, be made up of each component of following masses number: cactus juice 30-33 part, oenanthe stolonifera 21-23 part, infusion 20-21 part of garden burnet, Pu'er cooked tea 13-15 part, morning glory 20-22 part, sealwort 19-21 part, Fructus Sophorae 18-20 part, Mazus japonicus 18-20 part, field thistle 17-19 part, achene of Siberian cocklebur 16-18 part, selfheal 15-16 part, black nightshade 14-16 part, fleece-flower root 14-16 part, root of bidentate achyranthes 14-16 part, rhizoma atractylodis 13-15 part, dandelion 12-14 part, szechwan raspberry root or leaf 12-14 part, khas aletris herb 12-14 part, ginger 10-12 part, nettle 9-12 part, bamboo salt 9-12 part, euphausia superba powder 15-17 part, dry earthworm powder 13-15 part, dry clam worm powder 13-15 part, fish oil 14-16 part, honey 14-16 part, soybean oil 10-12 part, the described fleece-flower root is identical with the mass fraction of the root of bidentate achyranthes, described szechwan raspberry root or leaf is identical with the mass fraction of khas aletris herb.
Preferably, the feed addictive of described prevention angle fish scales upright, be made up of each component of following masses number: cactus juice 32 parts, oenanthe stolonifera 22 parts, the infusion of garden burnet 21 parts, Pu'er cooked tea 14 parts, 21 parts, morning glory, sealwort 20 parts, the Fructus Sophorae 19 parts, Mazus japonicus 19 parts, field thistle 18 parts, the achene of Siberian cocklebur 17 parts, selfheal 15 parts, black nightshade 15 parts, the fleece-flower root 15 parts, the root of bidentate achyranthes 15 parts, rhizoma atractylodis 14 parts, dandelion 13 parts, szechwan raspberry root or leaf 13 parts, khas aletris herb 13 parts, 11 parts, ginger, nettle 10 parts, bamboo salt 10 parts, euphausia superba powder 16 parts, dry earthworm powder 14 parts, 14 parts dry, clam worm powder, 15 parts, fish oil, honey 15 parts, soybean oil 11 parts.
Another object of the present invention is to provide a kind of preparation method of feed addictive of above-mentioned prevention angle fish scales upright, and the preparation method of the feed addictive of this prevention angle fish scales upright comprises the following steps:
(1) take oenanthe stolonifera, Pu'er cooked tea and morning glory by proportioning, the gross weight of oenanthe stolonifera, Pu'er cooked tea and morning glory is denoted as 1 part, takes the purified water of 10 parts, at 70-80 DEG C, 2-3h is soaked in water-bath, obtains soak;
(2) boil 2h after the soak that step (1) obtains being heated to boiling, be separated and take out fried liquid, continue the purified water adding 5 parts in medicinal material, continue to boil 1.5h after being heated to boiling, merge twice fried liquid and be extract;
(3) take sealwort, the Fructus Sophorae, Mazus japonicus, field thistle, the achene of Siberian cocklebur, selfheal, black nightshade, the fleece-flower root, the root of bidentate achyranthes, rhizoma atractylodis, dandelion, ginger and nettle respectively by said ratio, load ball mill grinding 2h, cross 80 mesh sieves, obtain grounds travel;
(4) grounds travel that the extract that obtains of step (2) and step (3) obtain is positioned in the extraction kettle in supercritical extract instrument, carbon dioxide is utilized to extract as medium, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure 12Mpa, separator temperature is 70 DEG C, extracts 2 hours, is extracted liquid;
(5) take szechwan raspberry root or leaf and khas aletris herb according to proportioning, smash to pieces together, make popular with wine, the szechwan raspberry root or leaf that must make popular and khas aletris herb;
(6) cactus juice, the infusion of garden burnet, bamboo salt, euphausia superba powder, dry earthworm powder, dry clam worm powder, fish oil, honey and soybean oil is taken according to proportioning, mix with the extract of step (4) gained, the szechwan raspberry root or leaf made popular of step (5) gained and khas aletris herb, obtain object.
The preparation method of described cactus juice is: smashed to pieces by cactus or grind or squeeze the juice with juice extractor, and gained juice is cactus juice.
The preparation method of the infusion of described garden burnet is: cleaned by garden burnet, drain the water, and adds the purified water that parts by weight are 4 times of garden burnet parts by weight, soak at room temperature 24-48h, filters to obtain the infusion of garden burnet.
The preparation method of the earthworm powder of described drying is: cleaned by earthworm fresh water, then uses distilled water flushing three times, and vacuum freeze drying, to finish-drying, is crossed 80 mesh sieves after pulverizing, obtain dry earthworm powder with pulverizer.The advantage of vacuum freeze drying is thoroughly dry, can not make the active material inactivation in earthworm body in dry process.
The preparation method of the clam worm powder of described drying is: cleaned by clam worm fresh water, then uses distilled water flushing three times, and vacuum freeze drying, to finish-drying, is crossed 80 mesh sieves after pulverizing, obtain dry clam worm powder with pulverizer.The advantage of vacuum freeze drying is thoroughly dry, can not make the active material inactivation in clam worm body in dry process.
The using method of the feed addictive of prevention angle fish scales upright of the present invention is: throw something and feed after being mixed with the weight ratio of 1:30-1:10 with commercially available basal feed by the feed addictive of prevention angle fish scales upright of the present invention, preferably, the feed addictive of prevention angle fish scales upright of the present invention and the weight ratio of commercially available basal feed are 1:20.
Each component effect of the present invention is resolved as follows:
The sweet property of cactus taste is put down, tonifying spleen stomach invigorating, beneficial strength of one's legs, except rushing down for a long time, can also be clearing heat and detoxicating, and scattered silt is subsided a swelling, benefiting stomach and stopping pain, and antibechic is interior with controlling taste-blindness rate, acute dysentery, cough; Mumps is controlled in external application, mastitis, carbuncle furuncle poison, snake bite, burn and scald.Oenanthe stolonifera belongs to Umbelliferae, Chinese celery Lepidium, for aquatic persistent root herbs for many years, oenanthe stolonifera taste sweet pungent, cool in nature, enter lung, stomach warp, there are clearing heat and detoxicating, foster lean gas, blood clean, reduce blood pressure, declare the effects such as lung dampness removing, also can control the illnesss such as urine leaching pain, fecal blood, jaundice, acute toothache, mumps.Garden burnet another name " yellow pawl is fragrant ", " beautiful letter ", " beautiful fermented soya beans, salted or other wise " or " acid is reddish brown " etc., cold in nature, bitter acid, nontoxic; Return liver, lung, kidney and large intestine channel, have cooling blood and hemostasis, clearing heat and detoxicating, train clear yin-nourishing, the effects such as detumescence sore.Pu'er cooked tea includes abundant Tea Polyphenols, and Pu'er cooked tea has anticancer, inhibiting bacteria and diminishing inflammation, alleviates cigarette poison, alleviates the effects such as heavy metal poison, can treat bacillary dysentery.Morning glory has water pouring diuresis, effect by phlegm, detumescence, desinsection expelling parasite, cures mainly the diseases such as edema of limbs and trunks, nephritic dropsy, cirrhotic ascites, constipation, abdominal pain due to enterositosis.Sealwort can be used for treating dermatitis rhus, the favus of the scalp, and carbuncle swells, scrofula, carious tooth, and children's's breast is long-pending, internal traumatic injury.The Fructus Sophorae is dry flower and alabastrum, the ripening fruits of legume Chinese scholartree, energy clearing heat-fire, cooling blood and hemostasis, and can be used for intestines heat and have blood in stool, hemorrhoid swell hemorrhage, the headache of liver heat, dizzy hot eyes etc.Mazus japonicus is annual herb, energy pain relieving, stomach invigorating, and removing toxic substances, can be used for antimigraine, indigestion; Furunculosis is controlled in external application, impetigo, scalds.Field thistle leaf is sweet, cool in nature, energy cooling blood and hemostasis, removing toxicity for eliminating carbuncles, clearing heat and relieving fidgetness, field thistle complete stool is containing compositions such as choline, Catecholamine matter, saponin, alkaloids, and herb, containing compositions such as volatile oil, synanthrin, alkaloid, flavonoids, coumarin derivatives, all has inhibitory action to Diplococcus pneumopniae, hemolytic streptococcus, corynebacterium diphtheriae, typhoid fever village bacterium, Pseudomonas aeruginosa, shigella dysenteriae, staphylococcus aureus etc.Achene of Siberian cocklebur expelling cold, eliminating, clearing the nasal passage, wines used as antirheumatic, pain relieving.Selfheal another name wheat head selfheal, iron wire selfheal, heat-clearing can go irascibility, again can mass dissipating and swelling eliminating, can control acute mastitis and swell and ache.Black nightshade is annual herb plant, and complete stool is used as medicine, can eliminating stasis to subdue swelling, clearing heat and detoxicating.The fleece-flower root has another name called spends more knotweed, purple black rattan, Polygonum multiflower knotweed etc., and its block root is used as medicine, and can calm the nerves, nourish blood, active, and removing toxic substances (preventing malaria), disappear carbuncle; RADIX POLYGONI MULTIFLORI PREPARATA can enrich and benefit essence and blood, black beard and hair, strengthening the bones and muscles, filling liver kidney.Root of bidentate achyranthes another name ox knocks knee, Amaranthaceae, Achyranthes perennial herb, and root is used as medicine, activating blood to promote menstruation; Postpartum abdominal pain can be controlled, irregular menstruation, amenorrhoea, nosebleed epistaxis, toothache caused by fire of deficiency type, beriberi oedema; Energy filling liver kidney, strong waist knee; Control soreness of waist and knee joint, caused by liver and kidney deficiency, fall and beat stasis of blood pain, animal doctor, as controlling ox weak foot disease, gets injured by a fall knochenbruch etc.Rhizoma atractylodis cure mainly drying damp and strengthening spleen; Wines used as antirheumatic; Improving eyesight, main the spleen and stomach being stranded by dampness; Burnout is addicted to sleeping; Born of the same parents' ruffian abdominal distension; Poor appetite; Tell and have loose bowels; Phlegm and retained fluid; Wet swollen; Illness that has not attacked the vital organs of the human body folder is wet; The heavy pain of head body; Bi Zheng warm nature; Podomere is ached and is weighed; Impotence folds in a garment; Yctalopia.Dandelion energy bactericidal antiphlogistic, clearing heat and detoxicating, diuresis dissipating bind, controls acute mastitis, adenolymphitis, scrofula, malignant boil carbuncle toxin, acute conjunctivitis, cold, fever, acute tonsillitis, acute bronchitis, gastritis, hepatitis, cholecystitis, urinary tract infections.Szechwan raspberry root or leaf can treat over-strained hemoptysis, hemoptysis, irregular menstruation, dysentery, scrofula, fracture.Khas aletris herb is perennial herb, height about 30 centimetres, and its taste is pungent; Micro-hardship; Warm in nature, can qi-restoratives arrest sweating; Hemostasis, the empty hidrosis of main body; Night sweat; Neurasthenia; Spit blood; Have blood in stool.Ginger has bactericidal effect, and can strengthen digestion power.Nettle is a kind of herbaceos perennial, arduous, cold, poisonous, can treating rheumatic arthritis, postpartum exhausting, child convulsion, nettle rash, venomous snake bite.Bamboo salt is evaporated brine day to load in triennial blue or green bamboo, and two ends are sealed with natural loess, take pine tree as fuel, the material extract after 1000 DEG C ~ 1500 DEG C high-temperature calcinations, has antibacterial, anti-inflammatory, toxopexic effect.Euphausia superba powder is the nutrient source of good protein; average protein content accounts for about 60%; and the astaxanthin contained in euphausia superba powder serves fundamental role in the reactive oxygen species of protection fish, the beta carotene contained in euphausia superba powder and astaxanthin play an important role in the immune system regulating fish.Containing the multiple medicinal ingredient such as earthworm element, earthworm toxolysin, xanthine, antihistaminicum and Cobastab in earthworm body, and to angle fish, there is food calling effect.Clam worm delicious flavour, nutritious, it is rich in the multiple nutritional components such as protein and calcium, phosphorus, iron, iodine, and has higher medicinal and food therapy value, and property is salty-cold, has nourishing Yin and falling fire, the function of qi-restoratives clearing lung-heat have hypotensive, control wet, protective effect on cancer risk.It is cold in nature, and taste is sweet, salty, has effect that is nourishing Yin and falling fire, clearing lung-heat qi-restoratives.Fish oil can provide energy for angle fish, and has food calling effect.Honey can relax bowel, can make up strength rapidly, eliminates tired out, builds up one's resistance to disease, can antibacterial sterilizing, and can improve feed mouthfeel.Soybean oil can provide energy for angle fish.
In preparation method, bulk drug purified water soaking a period of time, medicinal material deliquescing, cell expansion, active ingredient is easily fried, and medicinal material contains starch, protein, after soaking, prevent when decocting, the starch gelatinization on medicinal material surface, protein coagulating, the capillary channel on blocking medicinal material surface, make the moisture when decocting enter not go, active ingredient is not easily fried, affects curative effect of medication; Decoction can change the irritation effect of medicine, relatively mildly acts on angle fish, promotes that angle fish is to the absorption of medicine; Grinding makes the starch in medicinal material, protein is separated out more fully at subsequent step; Supercritical extract, immersion, concentration step retain the active ingredient in each raw material of the present invention to greatest extent.
The feed addictive of prevention angle fish scales upright of the present invention, the mechanism of action according to scales upright, the suitable component selected, and organically combined, there is significant cooperative synergism effect, play the function of each component to greatest extent, and the component of the feed addictive of prevention angle fish scales upright of the present invention is environmental protection raw material, environmental sound.Because the fish oil in feed addictive of the present invention has food calling effect, euphausia superba powder, dry earthworm powder and dry clam worm delicious flavour, honey can be in harmonious proportion and improve feed mouthfeel, soybean oil can provide energy for angle fish, therefore feed addictive of the present invention can stimulate the taste bud of angle fish, induction angle fish ingests and with the addition of the basal feed of feed addictive of the present invention, therefore angle fish immunity and resistance can be strengthened in feed addictive of the present invention, the composition pathogen of angle fish scales upright to remarkable bactericidal and bacteriostatic effect can enter in angle fish body, and play it and act on accordingly.
Compared with prior art, the invention has the beneficial effects as follows:
(1) the sweet property of cactus taste is put down, tonifying spleen stomach invigorating, beneficial strength of one's legs, except rushing down for a long time, can also be clearing heat and detoxicating, and scattered silt is subsided a swelling, benefiting stomach and stopping pain, and antibechic is interior with controlling taste-blindness rate, acute dysentery, cough; Mumps is controlled in external application, mastitis, carbuncle furuncle poison, snake bite, burn and scald.Oenanthe stolonifera belongs to Umbelliferae, Chinese celery Lepidium, for aquatic persistent root herbs for many years, oenanthe stolonifera taste sweet pungent, cool in nature, enter lung, stomach warp, there are clearing heat and detoxicating, foster lean gas, blood clean, reduce blood pressure, declare the effects such as lung dampness removing, also can control the illnesss such as urine leaching pain, fecal blood, jaundice, acute toothache, mumps.Garden burnet another name " yellow pawl is fragrant ", " beautiful letter ", " beautiful fermented soya beans, salted or other wise " or " acid is reddish brown " etc., cold in nature, bitter acid, nontoxic; Return liver, lung, kidney and large intestine channel, have cooling blood and hemostasis, clearing heat and detoxicating, train clear yin-nourishing, the effects such as detumescence sore.Pu'er cooked tea includes abundant Tea Polyphenols, and Pu'er cooked tea has anticancer, inhibiting bacteria and diminishing inflammation, alleviates cigarette poison, alleviates the effects such as heavy metal poison, can treat bacillary dysentery.Morning glory has water pouring diuresis, effect by phlegm, detumescence, desinsection expelling parasite, cures mainly the diseases such as edema of limbs and trunks, nephritic dropsy, cirrhotic ascites, constipation, abdominal pain due to enterositosis.Sealwort can be used for treating dermatitis rhus, the favus of the scalp, and carbuncle swells, scrofula, carious tooth, and children's's breast is long-pending, internal traumatic injury.The Fructus Sophorae is dry flower and alabastrum, the ripening fruits of legume Chinese scholartree, energy clearing heat-fire, cooling blood and hemostasis, and can be used for intestines heat and have blood in stool, hemorrhoid swell hemorrhage, the headache of liver heat, dizzy hot eyes etc.Mazus japonicus is annual herb, energy pain relieving, stomach invigorating, and removing toxic substances, can be used for antimigraine, indigestion; Furunculosis is controlled in external application, impetigo, scalds.Field thistle leaf is sweet, cool in nature, energy cooling blood and hemostasis, removing toxicity for eliminating carbuncles, clearing heat and relieving fidgetness, field thistle complete stool is containing compositions such as choline, Catecholamine matter, saponin, alkaloids, and herb, containing compositions such as volatile oil, synanthrin, alkaloid, flavonoids, coumarin derivatives, all has inhibitory action to Diplococcus pneumopniae, hemolytic streptococcus, corynebacterium diphtheriae, typhoid fever village bacterium, Pseudomonas aeruginosa, shigella dysenteriae, staphylococcus aureus etc.Achene of Siberian cocklebur expelling cold, eliminating, clearing the nasal passage, wines used as antirheumatic, pain relieving.Selfheal another name wheat head selfheal, iron wire selfheal, heat-clearing can go irascibility, again can mass dissipating and swelling eliminating, can control acute mastitis and swell and ache.Black nightshade is annual herb plant, and complete stool is used as medicine, can eliminating stasis to subdue swelling, clearing heat and detoxicating.The fleece-flower root has another name called spends more knotweed, purple black rattan, Polygonum multiflower knotweed etc., and its block root is used as medicine, and can calm the nerves, nourish blood, active, and removing toxic substances (preventing malaria), disappear carbuncle; RADIX POLYGONI MULTIFLORI PREPARATA can enrich and benefit essence and blood, black beard and hair, strengthening the bones and muscles, filling liver kidney.Root of bidentate achyranthes another name ox knocks knee, Amaranthaceae, Achyranthes perennial herb, and root is used as medicine, activating blood to promote menstruation; Postpartum abdominal pain can be controlled, irregular menstruation, amenorrhoea, nosebleed epistaxis, toothache caused by fire of deficiency type, beriberi oedema; Energy filling liver kidney, strong waist knee; Control soreness of waist and knee joint, caused by liver and kidney deficiency, fall and beat stasis of blood pain, animal doctor, as controlling ox weak foot disease, gets injured by a fall knochenbruch etc.Rhizoma atractylodis cure mainly drying damp and strengthening spleen; Wines used as antirheumatic; Improving eyesight, main the spleen and stomach being stranded by dampness; Burnout is addicted to sleeping; Born of the same parents' ruffian abdominal distension; Poor appetite; Tell and have loose bowels; Phlegm and retained fluid; Wet swollen; Illness that has not attacked the vital organs of the human body folder is wet; The heavy pain of head body; Bi Zheng warm nature; Podomere is ached and is weighed; Impotence folds in a garment; Yctalopia.Dandelion energy bactericidal antiphlogistic, clearing heat and detoxicating, diuresis dissipating bind, controls acute mastitis, adenolymphitis, scrofula, malignant boil carbuncle toxin, acute conjunctivitis, cold, fever, acute tonsillitis, acute bronchitis, gastritis, hepatitis, cholecystitis, urinary tract infections.Szechwan raspberry root or leaf can treat over-strained hemoptysis, hemoptysis, irregular menstruation, dysentery, scrofula, fracture.Khas aletris herb is perennial herb, height about 30 centimetres, and its taste is pungent; Micro-hardship; Warm in nature, can qi-restoratives arrest sweating; Hemostasis, the empty hidrosis of main body; Night sweat; Neurasthenia; Spit blood; Have blood in stool.Ginger has bactericidal effect, and can strengthen digestion power.Nettle is a kind of herbaceos perennial, arduous, cold, poisonous, can treating rheumatic arthritis, postpartum exhausting, child convulsion, nettle rash, venomous snake bite.Bamboo salt is evaporated brine day to load in triennial blue or green bamboo, and two ends are sealed with natural loess, take pine tree as fuel, the material extract after 1000 DEG C ~ 1500 DEG C high-temperature calcinations, has antibacterial, anti-inflammatory, toxopexic effect.Euphausia superba powder is the nutrient source of good protein; average protein content accounts for about 60%; and the astaxanthin contained in euphausia superba powder serves fundamental role in the reactive oxygen species of protection fish, the beta carotene contained in euphausia superba powder and astaxanthin play an important role in the immune system regulating fish.Containing the multiple medicinal ingredient such as earthworm element, earthworm toxolysin, xanthine, antihistaminicum and Cobastab in earthworm body, and to angle fish, there is food calling effect.Clam worm delicious flavour, nutritious, it is rich in the multiple nutritional components such as protein and calcium, phosphorus, iron, iodine, and has higher medicinal and food therapy value, and property is salty-cold, has nourishing Yin and falling fire, the function of qi-restoratives clearing lung-heat have hypotensive, control wet, protective effect on cancer risk.It is cold in nature, and taste is sweet, salty, has effect that is nourishing Yin and falling fire, clearing lung-heat qi-restoratives.Fish oil can provide energy for angle fish, and has food calling effect.Honey can relax bowel, can make up strength rapidly, eliminates tired out, builds up one's resistance to disease, can antibacterial sterilizing, and can improve feed mouthfeel.Soybean oil can provide energy for angle fish.
(2) feed addictive of prevention angle fish scales upright of the present invention, the mechanism of action according to scales upright, the suitable component selected, and organically combined, there is significant cooperative synergism effect, play the function of each component to greatest extent, and the component of the feed addictive of prevention angle fish scales upright of the present invention is environmental protection raw material, environmental sound.Because the fish oil in feed addictive of the present invention has food calling effect, euphausia superba powder, dry earthworm powder and dry clam worm delicious flavour, honey can be in harmonious proportion and improve feed mouthfeel, soybean oil can provide energy for angle fish, therefore feed addictive of the present invention can stimulate the taste bud of angle fish, induction angle fish ingests and with the addition of the basal feed of feed addictive of the present invention, therefore angle fish immunity and resistance can be strengthened in feed addictive of the present invention, the composition pathogen of angle fish scales upright to remarkable bactericidal and bacteriostatic effect can enter in angle fish body, and play it and act on accordingly.
(3) in preparation method, bulk drug purified water soaking a period of time, medicinal material deliquescing, cell expansion, active ingredient is easily fried, and medicinal material contains starch, protein, after soaking, prevent when decocting, the starch gelatinization on medicinal material surface, protein coagulating, the capillary channel on blocking medicinal material surface, make the moisture when decocting enter not go, active ingredient is not easily fried, affects curative effect of medication; Decoction can change the irritation effect of medicine, relatively mildly acts on angle fish, promotes that angle fish is to the absorption of medicine; Grinding makes the starch in medicinal material, protein is separated out more fully at subsequent step; Supercritical extract, immersion, concentration step retain the active ingredient in each component of the present invention to greatest extent.
Detailed description of the invention
Following embodiment is convenient to understand the present invention better, but does not limit the present invention.Experimental technique in following embodiment, if no special instructions, is conventional method.
Embodiment 1
In the present invention's first detailed description of the invention, feed ingredient and preparation method thereof is as follows:
A kind of feed addictive preventing angle fish scales upright, be made up of each component of following masses number: cactus juice 35 parts, oenanthe stolonifera 25 parts, the infusion of garden burnet 23 parts, Pu'er cooked tea 18 parts, 23 parts, morning glory, sealwort 23 parts, the Fructus Sophorae 22 parts, Mazus japonicus 22 parts, field thistle 21 parts, the achene of Siberian cocklebur 20 parts, selfheal 18 parts, black nightshade 19 parts, the fleece-flower root 18 parts, the root of bidentate achyranthes 18 parts, rhizoma atractylodis 18 parts, dandelion 16 parts, szechwan raspberry root or leaf 16 parts, khas aletris herb 16 parts, 14 parts, ginger, nettle 15 parts, bamboo salt 15 parts, euphausia superba powder 19 parts, dry earthworm powder 17 parts, 17 parts dry, clam worm powder, 18 parts, fish oil, honey 18 parts, soybean oil 14 parts.
The preparation method of the feed addictive of above-mentioned prevention angle fish scales upright comprises the following steps:
(1) take oenanthe stolonifera, Pu'er cooked tea and morning glory by proportioning, the gross weight of oenanthe stolonifera, Pu'er cooked tea and morning glory is denoted as 1 part, takes the purified water of 10 parts, at 70-80 DEG C, 2-3h is soaked in water-bath, obtains soak;
(2) boil 2h after the soak that step (1) obtains being heated to boiling, be separated and take out fried liquid, continue the purified water adding 5 parts in medicinal material, continue to boil 1.5h after being heated to boiling, merge twice fried liquid and be extract;
(3) take sealwort, the Fructus Sophorae, Mazus japonicus, field thistle, the achene of Siberian cocklebur, selfheal, black nightshade, the fleece-flower root, the root of bidentate achyranthes, rhizoma atractylodis, dandelion, ginger and nettle respectively by said ratio, load ball mill grinding 2h, cross 80 mesh sieves, obtain grounds travel;
(4) grounds travel that the extract that obtains of step (2) and step (3) obtain is positioned in the extraction kettle in supercritical extract instrument, carbon dioxide is utilized to extract as medium, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure 12Mpa, separator temperature is 70 DEG C, extracts 2 hours, is extracted liquid;
(5) take szechwan raspberry root or leaf and khas aletris herb according to proportioning, smash to pieces together, make popular with wine, the szechwan raspberry root or leaf that must make popular and khas aletris herb;
(6) cactus juice, the infusion of garden burnet, bamboo salt, euphausia superba powder, dry earthworm powder, dry clam worm powder, fish oil, honey and soybean oil is taken according to proportioning, mix with the extract of step (4) gained, the szechwan raspberry root or leaf made popular of step (5) gained and khas aletris herb, obtain object.
The preparation method of described cactus juice is: smashed to pieces by cactus or grind or squeeze the juice with juice extractor, and gained juice is cactus juice.
The preparation method of the infusion of described garden burnet is: cleaned by garden burnet, drain the water, and adds the purified water that parts by weight are 4 times of garden burnet parts by weight, soak at room temperature 24-48h, filters to obtain the infusion of garden burnet.
The preparation method of the earthworm powder of described drying is: cleaned by earthworm fresh water, then uses distilled water flushing three times, and vacuum freeze drying, to finish-drying, is crossed 80 mesh sieves after pulverizing, obtain dry earthworm powder with pulverizer.
The preparation method of the clam worm powder of described drying is: cleaned by clam worm fresh water, then uses distilled water flushing three times, and vacuum freeze drying, to finish-drying, is crossed 80 mesh sieves after pulverizing, obtain dry clam worm powder with pulverizer.
Embodiment 2
In the present invention's second detailed description of the invention, feed ingredient and preparation method thereof is as follows:
A kind of feed addictive preventing angle fish scales upright, be made up of each component of following masses number: cactus juice 28 parts, oenanthe stolonifera 20 parts, the infusion of garden burnet 19 parts, Pu'er cooked tea 10 parts, 18 parts, morning glory, sealwort 17 parts, the Fructus Sophorae 17 parts, Mazus japonicus 16 parts, field thistle 15 parts, the achene of Siberian cocklebur 14 parts, selfheal 14 parts, black nightshade 12 parts, the fleece-flower root 12 parts, the root of bidentate achyranthes 12 parts, rhizoma atractylodis 10 parts, dandelion 10 parts, szechwan raspberry root or leaf 9 parts, khas aletris herb 9 parts, 8 parts, ginger, nettle 7 parts, bamboo salt 7 parts, euphausia superba powder 13 parts, dry earthworm powder 11 parts, 11 parts dry, clam worm powder, 12 parts, fish oil, honey 12 parts, soybean oil 8 parts.
The preparation method of the feed addictive of the prevention angle fish scales upright of the present embodiment is with embodiment 1.
Embodiment 3
In the present invention's the 3rd detailed description of the invention, feed ingredient and preparation method thereof is as follows:
A kind of feed addictive preventing angle fish scales upright, be made up of each component of following masses number: cactus juice 33 parts, oenanthe stolonifera 23 parts, the infusion of garden burnet 21 parts, Pu'er cooked tea 15 parts, 22 parts, morning glory, sealwort 21 parts, the Fructus Sophorae 20 parts, Mazus japonicus 20 parts, field thistle 19 parts, the achene of Siberian cocklebur 18 parts, selfheal 16 parts, black nightshade 16 parts, the fleece-flower root 16 parts, the root of bidentate achyranthes 16 parts, rhizoma atractylodis 13 parts, dandelion 12 parts, szechwan raspberry root or leaf 14 parts, khas aletris herb 14 parts, 12 parts, ginger, nettle 12 parts, bamboo salt 12 parts, euphausia superba powder 17 parts, dry earthworm powder 15 parts, 15 parts dry, clam worm powder, 16 parts, fish oil, honey 16 parts, soybean oil 12 parts.
The preparation method of the feed addictive of the prevention angle fish scales upright of the present embodiment is with embodiment 1.
Embodiment 4
In the present invention's the 4th detailed description of the invention, feed ingredient and preparation method thereof is as follows:
A kind of feed addictive preventing angle fish scales upright, be made up of each component of following masses number: cactus juice 30 parts, oenanthe stolonifera 19 parts, the infusion of garden burnet 20 parts, Pu'er cooked tea 13 parts, 20 parts, morning glory, sealwort 19 parts, the Fructus Sophorae 18 parts, Mazus japonicus 18 parts, field thistle 17 parts, the achene of Siberian cocklebur 16 parts, selfheal 15 parts, black nightshade 14 parts, the fleece-flower root 14 parts, the root of bidentate achyranthes 14 parts, rhizoma atractylodis 15 parts, dandelion 14 parts, szechwan raspberry root or leaf 12 parts, khas aletris herb 12 parts, 10 parts, ginger, nettle 9 parts, bamboo salt 10 parts, euphausia superba powder 15 parts, dry earthworm powder 13 parts, 13 parts dry, clam worm powder, 14 parts, fish oil, honey 14 parts, soybean oil 10 parts.
The preparation method of the feed addictive of the prevention angle fish scales upright of the present embodiment is with embodiment 1.
Embodiment 5
In the present invention's the 5th detailed description of the invention, feed ingredient and preparation method thereof is as follows:
A kind of feed addictive preventing angle fish scales upright, be made up of each component of following masses number: cactus juice 31 parts, oenanthe stolonifera 21 parts, the infusion of garden burnet 19 parts, Pu'er cooked tea 13 parts, 20 parts, morning glory, sealwort 18 parts, the Fructus Sophorae 19 parts, Mazus japonicus 18 parts, field thistle 19 parts, the achene of Siberian cocklebur 14 parts, selfheal 14 parts, black nightshade 12 parts, the fleece-flower root 18 parts, the root of bidentate achyranthes 18 parts, rhizoma atractylodis 10 parts, dandelion 14 parts, szechwan raspberry root or leaf 9 parts, khas aletris herb 9 parts, 10 parts, ginger, nettle 12 parts, bamboo salt 9 parts, euphausia superba powder 16 parts, dry earthworm powder 15 parts, 13 parts dry, clam worm powder, 14 parts, fish oil, honey 14 parts, soybean oil 10 parts.
The preparation method of the feed addictive of the prevention angle fish scales upright of the present embodiment is with embodiment 1.
Embodiment 6
In the present invention's the 6th detailed description of the invention, feed ingredient and preparation method thereof is as follows:
A kind of feed addictive preventing angle fish scales upright, be made up of each component of following masses number: cactus juice 32 parts, oenanthe stolonifera 22 parts, the infusion of garden burnet 21 parts, Pu'er cooked tea 14 parts, 21 parts, morning glory, sealwort 20 parts, the Fructus Sophorae 19 parts, Mazus japonicus 19 parts, field thistle 18 parts, the achene of Siberian cocklebur 17 parts, selfheal 15 parts, black nightshade 15 parts, the fleece-flower root 15 parts, the root of bidentate achyranthes 15 parts, rhizoma atractylodis 14 parts, dandelion 13 parts, szechwan raspberry root or leaf 13 parts, khas aletris herb 13 parts, 11 parts, ginger, nettle 10 parts, bamboo salt 10 parts, euphausia superba powder 16 parts, dry earthworm powder 14 parts, 14 parts dry, clam worm powder, 15 parts, fish oil, honey 15 parts, soybean oil 11 parts.
The preparation method of the feed addictive of the prevention angle fish scales upright of the present embodiment is with embodiment 1.
Experimental result
Preventive effect is tested
With basal feed group of throwing something and feeding (for normal commercial diet) for control group, to add the feed group of the embodiment of the present invention 6 in basal feed for experimental group, often group establish 3 parallel, in experimental group, the additive of embodiment 6 and the weight ratio of basal feed are 1:20.Choose 6 aquariums, healthy angle fish 60, healthy angle fish is all assigned to 6 aquariums at random, each aquarium 10 angle fish, add the angle fish that 1 is suffered from scales upright in each aquarium.The results are shown in Table 1:
The effect situation of table 1 feed addictive prevention of the present invention scales upright
From table 1, feed addictive of the present invention effectively can reduce the incidence rate of angle fish scales upright, improves the immunity of angle fish, has good prevention effect to scales upright.
Bacteriostatic test checks
With the mixed liquor of 5ppm copper sulphate, 2ppm ferrous sulfate and 10ppm bleaching powder as control group, the feed addictive of the prevention angle fish scales upright of the embodiment of the present invention 6 is that experimental group carries out antibacterial inspection.
The preparation of culture medium: take in the water-soluble 800mL of beef extract 5g, adds peptone 10g and dissolves, and adds casein 34g and dissolves, then adds NaCl5g dissolving, is adjusted to pH7.6 ~ 7.8, is diluted to 1000mL.Add agar powder 18g, autoclaving 15min, when being cooled to 40 DEG C ~ 50 DEG C, a kind of rhyme scheme in Chinese operas serving as the prelude to a complete score for voices, solidifies, and the 4 DEG C of refrigerators placing no bacteria pollution are for subsequent use.
Bacterial classification is: small-sized point-like pole hair bacillus.
Control group antibacterial liquid is prepared: 1 part of 5ppm copper sulphate, 2ppm ferrous sulfate and 10ppm bleaching powder mixed liquor are dissolved in 10ml aseptic deionized water, are diluted respectively by liquor strength, with stoste than being 1:2,1:4,1:8,1:16,1:32.
Experimental group antibacterial liquid is prepared: be dissolved in 10ml aseptic deionized water by the feed addictive of the prevention angle fish scales upright of the embodiment of the present invention 6, diluted respectively by liquor strength, with stoste than being 1:2,1:4,1:8,1:16,1:32.To the fungistatic effect of small-sized point-like pole hair bacillus in table 3:
The bactericidal and bacteriostatic effect of the feed addictive of table 2 prevention of the present invention angle fish scales upright
From table 2, the pathogen of feed addictive to the scales upright small-sized point-like pole hair bacillus of the prevention angle fish scales upright of embodiments of the invention 1 has good bactericidal and bacteriostatic effect.
The above is only the preferred embodiment of the present invention, is noted that for those skilled in the art, and under the premise without departing from the principles of the invention, can also make some improvement, these improvement should be considered as protection scope of the present invention.
Claims (10)
1. one kind is prevented the feed addictive of angle fish scales upright, it is characterized in that: be made up of each component of following masses number: cactus juice 28-35 part, oenanthe stolonifera 19-25 part, infusion 19-23 part of garden burnet, Pu'er cooked tea 10-18 part, morning glory 18-23 part, sealwort 17-23 part, Fructus Sophorae 17-22 part, Mazus japonicus 16-22 part, field thistle 15-21 part, achene of Siberian cocklebur 14-20 part, selfheal 14-18 part, black nightshade 12-19 part, fleece-flower root 12-18 part, root of bidentate achyranthes 12-18 part, rhizoma atractylodis 10-18 part, dandelion 10-16 part, szechwan raspberry root or leaf 9-16 part, khas aletris herb 9-16 part, ginger 8-14 part, nettle 7-15 part, bamboo salt 7-15 part, euphausia superba powder 13-19 part, dry earthworm powder 11-17 part, dry clam worm powder 11-17 part, fish oil 12-18 part, honey 12-18 part, soybean oil 8-14 part, the described fleece-flower root is identical with the mass fraction of the root of bidentate achyranthes, described szechwan raspberry root or leaf is identical with the mass fraction of khas aletris herb.
2. the feed addictive of prevention angle fish scales upright according to claim 1, is characterized in that: the feed addictive of described prevention angle fish scales upright, is made up of each component of following masses number: cactus juice 30-33 part, oenanthe stolonifera 21-23 part, infusion 20-21 part of garden burnet, Pu'er cooked tea 13-15 part, morning glory 20-22 part, sealwort 19-21 part, Fructus Sophorae 18-20 part, Mazus japonicus 18-20 part, field thistle 17-19 part, achene of Siberian cocklebur 16-18 part, selfheal 15-16 part, black nightshade 14-16 part, fleece-flower root 14-16 part, root of bidentate achyranthes 14-16 part, rhizoma atractylodis 13-15 part, dandelion 12-14 part, szechwan raspberry root or leaf 12-14 part, khas aletris herb 12-14 part, ginger 10-12 part, nettle 9-12 part, bamboo salt 9-12 part, euphausia superba powder 15-17 part, dry earthworm powder 13-15 part, dry clam worm powder 13-15 part, fish oil 14-16 part, honey 14-16 part, soybean oil 10-12 part, the described fleece-flower root is identical with the mass fraction of the root of bidentate achyranthes, and described szechwan raspberry root or leaf is identical with the mass fraction of khas aletris herb.
3. the feed addictive of prevention angle fish scales upright according to claim 1, it is characterized in that: the feed addictive of described prevention angle fish scales upright, be made up of each component of following masses number: cactus juice 32 parts, oenanthe stolonifera 22 parts, the infusion of garden burnet 21 parts, Pu'er cooked tea 14 parts, 21 parts, morning glory, sealwort 20 parts, the Fructus Sophorae 19 parts, Mazus japonicus 19 parts, field thistle 18 parts, the achene of Siberian cocklebur 17 parts, selfheal 15 parts, black nightshade 15 parts, the fleece-flower root 15 parts, the root of bidentate achyranthes 15 parts, rhizoma atractylodis 14 parts, dandelion 13 parts, szechwan raspberry root or leaf 13 parts, khas aletris herb 13 parts, 11 parts, ginger, nettle 10 parts, bamboo salt 10 parts, euphausia superba powder 16 parts, dry earthworm powder 14 parts, 14 parts dry, clam worm powder, 15 parts, fish oil, honey 15 parts, soybean oil 11 parts.
4. a preparation method for the feed addictive of the prevention angle fish scales upright according to any one of claim 1-3, is characterized in that: comprise the following steps:
(1) take oenanthe stolonifera, Pu'er cooked tea and morning glory by proportioning, the gross weight of oenanthe stolonifera, Pu'er cooked tea and morning glory is denoted as 1 part, takes the purified water of 10 parts, at 70-80 DEG C, 2-3h is soaked in water-bath, obtains soak;
(2) boil 2h after the soak that step (1) obtains being heated to boiling, be separated and take out fried liquid, continue the purified water adding 5 parts in medicinal material, continue to boil 1.5h after being heated to boiling, merge twice fried liquid and be extract;
(3) take sealwort, the Fructus Sophorae, Mazus japonicus, field thistle, the achene of Siberian cocklebur, selfheal, black nightshade, the fleece-flower root, the root of bidentate achyranthes, rhizoma atractylodis, dandelion, ginger and nettle respectively by said ratio, load ball mill grinding 2h, cross 80 mesh sieves, obtain grounds travel;
(4) grounds travel that the extract that obtains of step (2) and step (3) obtain is positioned in the extraction kettle in supercritical extract instrument, carbon dioxide is utilized to extract as medium, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure 12Mpa, separator temperature is 70 DEG C, extracts 2 hours, is extracted liquid;
(5) take szechwan raspberry root or leaf and khas aletris herb according to proportioning, smash to pieces together, make popular with wine, the szechwan raspberry root or leaf that must make popular and khas aletris herb;
(6) cactus juice, the infusion of garden burnet, bamboo salt, euphausia superba powder, dry earthworm powder, dry clam worm powder, fish oil, honey and soybean oil is taken according to proportioning, mix with the extract of step (4) gained, the szechwan raspberry root or leaf made popular of step (5) gained and khas aletris herb, obtain object.
5. the preparation method of the feed addictive of prevention angle fish scales upright according to claim 4, is characterized in that: the preparation method of described cactus juice is: smashed to pieces by cactus or grind or squeeze the juice with juice extractor, and gained juice is cactus juice.
6. the preparation method of the feed addictive of prevention angle fish scales upright according to claim 4, it is characterized in that: the preparation method of the infusion of described garden burnet is: cleaned by garden burnet, drain the water, add the purified water that parts by weight are 4 times of garden burnet parts by weight, soak at room temperature 24-48h, filters to obtain the infusion of garden burnet.
7. the preparation method of the feed addictive of prevention angle fish scales upright according to claim 4, it is characterized in that: the preparation method of the earthworm powder of described drying is: cleaned by earthworm fresh water, use distilled water flushing again three times, vacuum freeze drying, to finish-drying, crosses 80 mesh sieves after pulverizing with pulverizer.
8. the preparation method of the feed addictive of prevention angle fish scales upright according to claim 4, it is characterized in that: the preparation method of the clam worm powder of described drying is: cleaned by clam worm fresh water, use distilled water flushing again three times, vacuum freeze drying, to finish-drying, crosses 80 mesh sieves after pulverizing with pulverizer.
9. a using method for the feed addictive of the prevention angle fish scales upright according to any one of claim 1-3, is characterized in that: throw something and feed after being mixed with the weight ratio of 1:30-1:10 with commercially available basal feed by the feed addictive of prevention angle fish scales upright of the present invention.
10. a using method for the feed addictive of prevention angle fish scales upright according to claim 9, is characterized in that: the feed addictive of prevention angle fish scales upright of the present invention and the weight ratio of commercially available basal feed are 1:20.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110574826A (en) * | 2019-08-13 | 2019-12-17 | 河南科技大学 | Chinese herbal medicine feed additive for preventing koi scale protrusion disease and preparation method thereof |
| CN113367242A (en) * | 2021-06-10 | 2021-09-10 | 中国农业科学院草原研究所 | Method for extracting alkaloid from nettle and application of alkaloid as growth promoting additive |
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