CN105106893A - Chinese herbal medicine feed additive for preventing white head-mouth disease of xiphophorus helleri and preparation method of Chinese herbal medicine feed additive - Google Patents
Chinese herbal medicine feed additive for preventing white head-mouth disease of xiphophorus helleri and preparation method of Chinese herbal medicine feed additive Download PDFInfo
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Abstract
The invention relates to a Chinese herbal medicine feed additive for preventing the white head-mouth disease of xiphophorus helleri. The invention further relates to a preparation method of the Chinese herbal medicine feed additive for preventing the white head-mouth disease of xiphophorus helleri. The Chinese herbal medicine feed additive for preventing the white head-mouth disease of xiphophorus helleri is prepared from, by mass, 25-33 parts of aqueous extract of garden burnet, 22-28 parts of field thistle juice, 7-14 parts of pu-erh ripe tea, 20-26 parts of bitter herbs, 19-26 parts of courled mallow, 15-23 parts of morning glory, 19-25 parts of selfheal, 21-28 parts of Chinese honeylocust fruits, 17-25 parts of atractylodes lancea, 17-23 parts of astragalus sinicus, 6-10 parts of bamboo salt, 16-25 parts of dry red worm powder, 14-20 parts of dry earthworm powder, 14-19 parts of fish oil and the like. The Chinese herbal medicine feed additive for preventing the white head-mouth disease of xiphophorus helleri can effectively prevent the occurrence of the white head-mouth disease of xiphophorus helleri.
Description
Technical field
The invention belongs to field of Chinese herbal medicine technology, particularly relate to a kind of Chinese herbal feed additive preventing red swordfish white head and mouth fish disease; The invention still further relates to the preparation method of the Chinese herbal feed additive of the red swordfish white head and mouth fish disease of a kind of above-mentioned prevention.
Background technology
Along with the growth in the living standard of people, material life is enriched greatly, and the requirement that people live to amusement and recreation is more and more obvious, and staying at home or supporting pet fish beautiful in colour a bit in garden is best selection, takes up room hardly because raise pet fish; Economic cost can be determined according to people; Condition is relatively simple, has water.Cultivation of ornamental fish not only can cultivate the body and the mind, and cultivate one's taste, the anion discharged in water and steam also can filter and wet air, build good living environment to people.
Red swordfish body subfusiform.The original species body colour of occurring in nature is pale bluish green.Milter tail fin lower edge extends a needle-like fin ray, is commonly called as sword tail, and raun is without sword tail, and Xiphophorus helleri is indispensable kind in aquarium.By biological engineering, its body colour is abundanter, has red, white, blue etc.It represents fish red sword, blue or green sword, Bai Jian, the red sword of black fin, the white sword of black fin, Aicis galericulata sword etc.Its body is strong, easily raises, and no matter is that slightly acidic water or week-base water can adapt to.Omnivorousness, Lumbricus, blood worm, man-made feeds all can be fed.Cold hardiness is stronger.Well-grown in the water temperature of 20 degree, is suitable for raising water temperature 24 ~ 26 degree, and its frightened rear happiness is jumped, and spring is very strong, for safety, preferably adds a cover plate on aquarium top.
White head and mouth fish disease is the one of myxobacter, and the pathogen form of gill rot is closely similar.During morbidity, the necrocytosis around the forehead of sick fish and mouth, pigment disappears and performance white, and diseased region festers, and sometimes with canescence floss hair, thus presents " the white mouth of hoary hair " symptom.The sick fish that the water surface is travelling, symptom is particularly evident.After sick fish is dried up, symptom is just not remarkable.Serious sick fish, lesions position festers, and congested phenomenon appears in indivedual sick fish head portion, sometimes also shows the pathological changes reactions such as Rhizoma Euonymus, white smear, rotten tail, gill rot or the many mucus of whole body.The general body of sick fish is thin, blackout, accelerated breathing, inappetence, and swimming slowly, constantly emerges, namely dead soon.This disease is a kind of eruptive epidemic disease, and morbidity is exceedingly fast, and infects rapidly, can be all dead between one day.This sick epidemic season is obvious, generally in late May to early July, June is onset peak.Therefore, develop a kind of feedstuff of red swordfish white head and mouth fish disease or feed additive of effectively preventing and have important economic implications and market prospect.
Summary of the invention
An object of the present invention is to provide a kind of Chinese herbal feed additive preventing red swordfish white head and mouth fish disease, its raw material components is green pure natural material, good bacteriostasis and sterilization effect is had to the pathogen of red swordfish white head and mouth fish disease, effectively can reduce the sickness rate of red swordfish white head and mouth fish disease, improve immunity and the survival rate of red swordfish.
In order to solve the problems of the technologies described above, the present invention by the following technical solutions, a kind of Chinese herbal feed additive preventing red swordfish white head and mouth fish disease, be made up of each component of following masses number: infusion 25-33 part of Radix Sanguisorbae, Herba Cirsii juice 22-28 part, Folium camelliae assamicae (processed) 7-14 part, Herba Sonchi Oleracei 20-26 part, certain herbaceous plants with big flowers dish 19-26 part, morning glory 15-23 part, Spica Prunellae 19-25 part, Fructus Gleditsia 21-28 part, Rhizoma Atractylodis 17-25 part, Herba Astragali Melilotoidis (Herba Astragali Sinici) 17-23 part, Herba Houttuyniae 13-22 part, Flos Lonicerae 16-24 part, Fructus Cnidii 16-24 part, Galla Chinensis 14-22 part, Fructus Sophorae 15-24 part, Radix Scutellariae 15-22 part, Bulbus Allii 11-19 part, Herba Taraxaci 10-16 part, Rhizoma Zingiberis Recens 9-16 part, Herba Sedi 8-15 part, Rhizoma Kaempferiae 9-14 part, Fructus Seu Herba Pubescentis 7-13 part, Fructus Xanthii 6-11 part, bamboo salt 6-10 part, Herba Kalimeridis juice 5-11 part, winter honey 5-11 part, dry red line worm powder 16-25 part, dry Lumbricus powder 14-20 part, fish oil 14-19 part, described Herba Kalimeridis juice is identical with the mass fraction of winter honey.
Preferably, the Chinese herbal feed additive of the red swordfish white head and mouth fish disease of described prevention, be made up of each component of following masses number: infusion 29-30 part of Radix Sanguisorbae, Herba Cirsii juice 24-26 part, Folium camelliae assamicae (processed) 9-11 part, Herba Sonchi Oleracei 22-24 part, certain herbaceous plants with big flowers dish 22-24 part, morning glory 18-21 part, Spica Prunellae 22-23 part, Fructus Gleditsia 24-26 part, Rhizoma Atractylodis 19-22 part, Herba Astragali Melilotoidis (Herba Astragali Sinici) 19-21 part, Herba Houttuyniae 17-19 part, Flos Lonicerae 19-21 part, Fructus Cnidii 20-21 part, Galla Chinensis 16-19 part, Fructus Sophorae 18-19 part, Radix Scutellariae 17-20 part, Bulbus Allii 14-16 part, Herba Taraxaci 13-14 part, Rhizoma Zingiberis Recens 12-14 part, Herba Sedi 11-13 part, Rhizoma Kaempferiae 11-12 part, Fructus Seu Herba Pubescentis 9-11 part, Fructus Xanthii 8-10 part, bamboo salt 7-9 part, Herba Kalimeridis juice 7-9 part, winter honey 7-9 part, dry red line worm powder 18-20 part, dry Lumbricus powder 16-18 part, fish oil 16-17 part, described Herba Kalimeridis juice is identical with the mass fraction of winter honey.
Preferably, the Chinese herbal feed additive of the red swordfish white head and mouth fish disease of described prevention, be made up of each component of following masses number: the infusion of Radix Sanguisorbae 30 parts, Herba Cirsii juice 25 parts, Folium camelliae assamicae (processed) 10 parts, Herba Sonchi Oleracei 23 parts, certain herbaceous plants with big flowers dish 23 parts, 19 parts, morning glory, Spica Prunellae 22 parts, 25 parts, Fructus Gleditsia, Rhizoma Atractylodis 20 parts, Herba Astragali Melilotoidis (Herba Astragali Sinici) 20 parts, Herba Houttuyniae 18 parts, Flos Lonicerae 20 parts, Fructus Cnidii 21 parts, Galla Chinensis 17 parts, the Fructus Sophorae 18 parts, Radix Scutellariae 19 parts, 15 parts, Bulbus Allii, Herba Taraxaci 14 parts, 13 parts, Rhizoma Zingiberis Recens, Herba Sedi 12 parts, Rhizoma Kaempferiae 12 parts, Fructus Seu Herba Pubescentis 10 parts, Fructus Xanthii 9 parts, bamboo salt 8 parts, Herba Kalimeridis juice 8 parts, winter honey 8 parts, 19 parts dry, red line worm powder, dry Lumbricus powder 17 parts, 17 parts, fish oil.
Another object of the present invention is to provide the preparation method of the Chinese herbal feed additive of the red swordfish white head and mouth fish disease of a kind of above-mentioned prevention, and the preparation method of the Chinese herbal feed additive of the red swordfish white head and mouth fish disease of this prevention comprises the following steps:
(1) take Radix Sanguisorbae by proportioning, Radix Sanguisorbae is cleaned, drain the water, add the purified water that parts by weight are 4 times of Radix Sanguisorbae parts by weight, soak at room temperature 24-48h, filter to obtain the infusion of Radix Sanguisorbae;
(2) ground by Herba Cirsii or squeeze the juice with juice extractor, gained juice is Herba Cirsii juice, takes Herba Cirsii juice according to proportioning;
(3) take Folium camelliae assamicae (processed), Herba Sonchi Oleracei, certain herbaceous plants with big flowers dish, morning glory and Spica Prunellae by proportioning, the gross weight of Folium camelliae assamicae (processed), Herba Sonchi Oleracei, certain herbaceous plants with big flowers dish, morning glory and Spica Prunellae is denoted as 1 part, takes the purified water of 15 parts, at 70-80 DEG C, 2-3h is soaked in water-bath, obtains soak;
(4) boil 2h after the soak that step (3) obtains being heated to boiling, be separated and take out fried liquid, continue the purified water adding 6 parts in medical material, continue to boil 1.5h after being heated to boiling, merge twice fried liquid and be extracting solution;
(5) Fructus Gleditsia, Rhizoma Atractylodis, Herba Astragali Melilotoidis (Herba Astragali Sinici), Herba Houttuyniae, Flos Lonicerae, Fructus Cnidii, Galla Chinensis, the Fructus Sophorae, Radix Scutellariae, Bulbus Allii, Herba Taraxaci, Rhizoma Zingiberis Recens, Herba Sedi, Rhizoma Kaempferiae, Fructus Seu Herba Pubescentis and Fructus Xanthii is taken respectively by said ratio, load ball mill grinding 2h, cross 80 mesh sieves, obtain grounds travel;
(6) grounds travel that the extracting solution that obtains of step (4) and step (5) obtain is positioned in the extraction kettle in supercritical extraction instrument, carbon dioxide is utilized to extract as medium, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure 12Mpa, separator temperature is 70 DEG C, extracts 2 hours, is extracted liquid;
(7) bamboo salt, Herba Kalimeridis juice, winter honey, dry red line worm powder, dry Lumbricus powder and fish oil is taken according to proportioning, mix homogeneously with the infusion of the Radix Sanguisorbae of step (1) gained, the Herba Cirsii juice of step (2) gained, the extract of step (6) gained, obtain object.
The preparation method of described Herba Kalimeridis juice is: ground by Herba Kalimeridis or squeeze the juice with juice extractor, gained juice is Herba Kalimeridis juice.
The preparation method of the red line worm powder of described drying is: cleaned by red line worm fresh water, then uses distilled water flushing three times, grinds to form powdery after vacuum lyophilization to finish-drying, crosses 80 mesh sieves.The advantage of vacuum lyophilization is thoroughly dry, can not make the active substance inactivation in red line polypide in dry process.
The preparation method of the Lumbricus powder of described drying is: cleaned by Lumbricus fresh water, then uses distilled water flushing three times, grinds to form powdery after vacuum lyophilization to finish-drying, crosses 80 mesh sieves.The advantage of vacuum lyophilization is thoroughly dry, can not make the active substance inactivation in Lumbricus body in dry process.
The using method of the Chinese herbal feed additive of prevention of the present invention red swordfish white head and mouth fish disease is: throw something and feed after being mixed homogeneously with the weight ratio of 1:30-1:10 with commercially available normal feedstuff by the Chinese herbal feed additive of red for prevention of the present invention swordfish white head and mouth fish disease, preferably, the Chinese herbal feed additive of prevention of the present invention red swordfish white head and mouth fish disease and the weight ratio of commercially available normal feedstuff are 1:20.
Each component effect of the present invention is resolved as follows:
Radix Sanguisorbae another name " yellow pawl is fragrant ", " beautiful letter ", " beautiful fermented soya beans, salted or other wise " or " acid is reddish brown " etc., cold in nature, bitter in the mouth acid, nontoxic; Return liver, lung, kidney and large intestine channel, have cooling blood for hemostasis, heat-clearing and toxic substances removing, train clear yin nourishing, the effects such as detumescence sore.Little Artichoke leaf is sweet, cool in nature, energy cooling blood for hemostasis, removing toxicity for eliminating carbuncles, clearing heat and relieving fidgetness, Herba Cirsii Herb is containing compositions such as choline, Catecholamine matter, Saponin, alkaloids, and herb, containing compositions such as volatile oil, inulin, alkaloid, flavonoid, coumarin derivatives, all has inhibitory action to Diplococcus pneumoniae, Hemolytic streptococcus, diphtheria corynebacterium, typhoid fever village bacterium, bacillus pyocyaneus, dysentery bacterium, staphylococcus aureus etc.Folium camelliae assamicae (processed) includes abundant tea polyphenols, and Folium camelliae assamicae (processed) has anticancer, inhibiting bacteria and diminishing inflammation, alleviates tobacco poisoning, alleviates the effects such as heavy metal poison, can treat bacillary dysentery.Slightly bitter during Herba Sonchi Oleracei sense of taste is sweet, there are the effects such as antibacterial, antipyretic, antiinflammatory, improving eyesight.Certain herbaceous plants with big flowers dish energy clearing away heat and expelling wind, it contains a large amount of vitamin, after being absorbed by the body, energy enhancing body resistance, diseases prevention infection, when influenza virus is popular, eating this dish has preventive effect; Suffer from vexed getting angry, during the red red disease of eyes, this dish can make the product of auxiliary treatment; Can anthelmintic detoxify, it contains a large amount of Plant fiber, can promote gastrointestinal peristalsis, and promote digestive function, the row being beneficial to stool is rushed down, and has wide intestinal defecating feces excretion, therefore can remove body accumulation toxin, discharges intestinal parasite.Morning glory has removing water retention by purgation diuresis, effect by expectorant, detumescence, parasite killing anthelmintic, cures mainly the diseases such as limds edema, oedema due to nephritis, cirrhotic ascites, constipation, abdominal pain due to worm stagnation.The decoct of Spica Prunellae, water-leach liquor, alcohol-water leachate and alcohol leaching liquid all can obviously reduce laboratory animal blood pressure, and stem, leaf, fringe and herb all have hypotensive effect, but the effect of fringe is more obvious; The water decoction-alcohol sedimentation liquid mouse peritoneal injection of Spica Prunellae, has obvious antiinflammatory action; The decoct of Spica Prunellae all has certain inhibitory action to dysentery bacterium, Bacillus typhi, vibrio cholera, escherichia coli, Bacillus proteus, staphylococcus and Bacillus tuberculosis in vitro.Fructus Gleditsia has the effect of parasite killing, removing toxic substances: saponin element contained in Fructus Gleditsia is triterpene class and oligosaccharide, has antiinflammatory, antiulcer, disease-resistant change effect, also has anticancer and improves the effects such as acquired immune deficiency syndrome (AIDS) immunity.Rhizoma Atractylodis cure mainly drying damp and strengthening spleen; Wind-damp dispelling; Improving eyesight, main the spleen and stomach being stranded by dampness; Asthenia drowsiness; Born of the same parents' painful abdominal mass abdominal distention; Inappetence; Tell and have loose bowels; Phlegm retention; Damp-edema; Exterior syndrome folder is wet; Head heavy sensation of the body pain; Arthromyodynia warm nature; Podomere is ached and is weighed; Flaccidity folds in a garment; Nyctalopia.Herba Astragali Melilotoidis (Herba Astragali Sinici) cures mainly removing blood stasis, hemostasia and promoting granulation, and profit can support the five internal organs, improve visual and auditory acuity, hot blast of dispelling, makes us spry and light, and food is not minded for a long time, mends people, can stop hot malaria, flat stomach of invigorating blood circulation.Herba Houttuyniae has heat-clearing and toxic substances removing, eliminating carbuncle evacuation of pus, the effect of inducing diuresis for treating stranguria syndrome.Flos Lonicerae contains the pharmacological component such as chlorogenic acid, luteolin glycosides, stronger restraint is had to the various pathogens such as Hemolytic streptococcus, staphylococcus aureus and upper respiratory tract infection Causative virus etc., in addition also can enhancing immunity, antiearly pregnancy, hepatoprotective, antitumor, antiinflammatory, antipyretic, hemostasis (blood coagulation), suppress intestinal absorption cholesterol etc., its clinical application widely, can be used for the treatment of respiratory tract infection, bacillary dysentery, acute urinary, hypertension etc. more than 40 with other medicines compatibility and plant disease.Fructus Cnidii is the dry mature fruit of samphire cnidium monnieri, has resisting pathogenic microbes effect, and Fructus Cnidii all has inhibitory action to acrothesium floccosum, Gypsum Fibrosum sample microspore bacterium, ulotrichy microspore bacterium; Also there is antifungic action, Antifungal activities, insecticidal action.Galla Chinensis is cold in nature, sour in the mouth, puckery, has the effects such as pathogenic fire reducing of astringing the lung, relieving diarrhea with astringents, arresting sweating hemostasis, removing dampness sore, cures mainly the diseases such as chronic cough of deficiency lung, chronic diarrhea chronic dysentery, hemorrhoidal bleeding of having blood in stool, carbuncle sore tumefacting virus, Skin wet and rotten.The Fructus Sophorae is dry flower and alabastrum, the mature fruit of leguminous plant Chinese scholartree, energy clearing away heat-fire, cooling blood for hemostasis, and can be used for intestinal heat and have blood in stool, hemorrhoid swell hemorrhage, and liver-heat is had a headache, dizzy conjunctival congestion etc.The root of Radix Scutellariae is used as medicine, bitter in the mouth, cold in nature, there are heat clearing and damp drying, eliminating fire and detoxication, hemostasis, the effect such as antiabortive, cure mainly the diseases such as epidemic febrile disease, upper respiratory tract infection, cough due to lung-heat, damp and hot jaundice, pneumonia, dysentery, hemoptysis, conjunctival congestion, frequent fetal movement, hypertension, carbuncle furuncle, the clinical antibiotic property of Radix Scutellariae is better than Rhizoma Coptidis, and does not develop immunity to drugs.Bulbus Allii removing toxic substances parasite killing, reducing swelling and alleviating pain, antidiarrheal dysentery relieving, controls lung, anthelmintic, and also have warming the spleen warming the stomach in addition, control swollen ulcer drug, white bald tinea skin ulcer, dysentery is had loose bowels, pulmonary tuberculosis pertussis, ascarid pinworm, eating accumulation, coldness and pain in the epigastrium, edema distension.Herba Taraxaci energy bactericidal antiphlogistic, heat-clearing and toxic substances removing, diuresis eliminating stagnation, controls acute mastitis, lymphadenitis, scrofula, furuncle carbuncle toxin, acute conjunctivitis, cold, fever, acute tonsillitis, acute bronchitis, gastritis, hepatitis, cholecystitis, urinary tract infection.Rhizoma Zingiberis Recens has bactericidal effect, and can strengthen digestion power.Herba Sedi is the conventional medicinal herbs that folklore is extremely wide simply, and all herbal medicine, has effect of heat-clearing and toxic substances removing, swelling diuretic, evacuation of pus granulation promoting etc.Rhizoma Kaempferiae is the dry rhizome of zingiberaceous plant Rhizoma Kaempferiae, is excavated winter, and clean, remove fibrous root, section is dried, warming middle-JIAO for eliminating dampness, promoting the circulation of QI to relieve pain; Cure mainly acute gastroenteritis, dyspepsia, cold syndrome of the stomach pain, toothache, rheumatic arthritis, traumatic injury.Fructus Seu Herba Pubescentis all herbal medicine, controls the diseases such as dysfunctional uterine hemorrhage, cholecystitis, jaundice type hepatitis, headache due to common cold, stomachache, infantile malnutrition, acute catarrhal conjunctivitis, traumatic injury, treatment skin ulcer, sores ulceration of skin, Serpentis and lyssodexis, tinea pedis, the treatment of rotten head and hemorrhoid.Fructus Xanthii dispersing wind-cold, clearing the nasal passage, wind-damp dispelling, pain relieving.Bamboo salt is evaporated brine day to load in triennial QINGZHU, and two ends are sealed with natural loess, take pinaster as fuel, the material extract after 1000 DEG C ~ 1500 DEG C high-temperature calcinations, has antibacterial, antiinflammatory, toxopexic effect.Herba Kalimeridis is dry herb or the root of feverfew Herba Kalimeridis, and summer, autumn gather, using fresh herb or dry; Its acrid in the mouth, cool in nature, there is cooling blood for hemostasis, clearing away heat-damp and promoting diuresis, effect of removing toxic substances and promoting subsidence of swelling, can be used for spitting blood, epistaxis, dysentery, metrorrhagia, wound hemorrhage, jaundice, edema, stranguria with turbid discharge, flu, cough, pharyngalgia sore throat, hemorrhoid, carbuncle, erysipelas, infantile malnutrition.Winter honey refers to the Mel gathered in the crops in the winter time, has the effect of removing toxic substances, doctor's skin ulcer, pain relieving; Mel is directly embrocated on skin or wound, has the effect of antiinflammatory, pain relieving, stop blooding, alleviate edema, promotion wound healing; Eat and can alleviate alimentary toxicosis, treatment gastroenteritic ulcer and tripe abdomen pain; In addition, winter honey can coordinating the actions of various ingredients in a prescription, improves the property of medicine.Red line worm can be used as the high-quality animal feed of aquatic animal, can reduce the production cost of feedstuff, improves culture benefit and forms quality.Containing the multiple medicinal ingredient such as Pheretima element, Pheretima toxolysin, xanthine, antihistaminic and vitamin B in Lumbricus body, and to red sword, there is food calling effect.Fish oil can provide energy for red sword, and has food calling effect.
In preparation method, crude drug purified water soaking a period of time, medical material deliquescing, cell expansion, effective ingredient is easily fried, and medical material contains starch, protein, after soaking, prevent when decocting, the starch gelatinization on medical material surface, protein coagulating, the capillary channel on blocking medical material surface, make the moisture when decocting enter not go, effective ingredient is not easily fried, affects curative effect of medication; Decoction can change the irritation effect of medicine, relatively mildly acts on red swordfish, promotes that red swordfish is to the absorption of medicine; Grinding makes the starch in medical material, protein is separated out more fully at subsequent step; Supercritical extraction, immersion, concentration step retain the effective ingredient in Chinese medicine of the present invention to greatest extent.
The Chinese herbal feed additive of the red swordfish white head and mouth fish disease of prevention of the present invention, the mechanism of action according to white head and mouth fish disease, the suitable Chinese herbal medicine selected, and organically combined, there is significant cooperative synergism effect, play the function of each component to greatest extent, and the component of the Chinese herbal feed additive of the red swordfish white head and mouth fish disease of prevention of the present invention is environmental protection raw material, environmental sound, has significant bactericidal and bacteriostatic effect to the pathogen of white head and mouth fish disease.Because the red line worm powder, Lumbricus powder and the fish oil that contain in Chinese herbal feed additive of the present invention have food calling effect, feedstuff mouthfeel can be improved to a certain extent, stimulate the taste bud of red swordfish, feedstuff of ingesting liked by red sword, therefore the composition that can strengthen red sword immunity and resistance, the pathogen of white head and mouth fish disease be had to remarkable bactericidal and bacteriostatic effect in additive of the present invention can enter in red sword body, and plays it and act on accordingly.
Compared with prior art, the invention has the beneficial effects as follows:
(1) Radix Sanguisorbae another name " yellow pawl is fragrant ", " beautiful letter ", " beautiful fermented soya beans, salted or other wise " or " acid is reddish brown " etc., cold in nature, and bitter in the mouth acid is nontoxic; Return liver, lung, kidney and large intestine channel, have cooling blood for hemostasis, heat-clearing and toxic substances removing, train clear yin nourishing, the effects such as detumescence sore.Little Artichoke leaf is sweet, cool in nature, energy cooling blood for hemostasis, removing toxicity for eliminating carbuncles, clearing heat and relieving fidgetness, Herba Cirsii Herb is containing compositions such as choline, Catecholamine matter, Saponin, alkaloids, and herb, containing compositions such as volatile oil, inulin, alkaloid, flavonoid, coumarin derivatives, all has inhibitory action to Diplococcus pneumoniae, Hemolytic streptococcus, diphtheria corynebacterium, typhoid fever village bacterium, bacillus pyocyaneus, dysentery bacterium, staphylococcus aureus etc.Folium camelliae assamicae (processed) includes abundant tea polyphenols, and Folium camelliae assamicae (processed) has anticancer, inhibiting bacteria and diminishing inflammation, alleviates tobacco poisoning, alleviates the effects such as heavy metal poison, can treat bacillary dysentery.Slightly bitter during Herba Sonchi Oleracei sense of taste is sweet, there are the effects such as antibacterial, antipyretic, antiinflammatory, improving eyesight.Certain herbaceous plants with big flowers dish energy clearing away heat and expelling wind, it contains a large amount of vitamin, after being absorbed by the body, energy enhancing body resistance, diseases prevention infection, when influenza virus is popular, eating this dish has preventive effect; Suffer from vexed getting angry, during the red red disease of eyes, this dish can make the product of auxiliary treatment; Can anthelmintic detoxify, it contains a large amount of Plant fiber, can promote gastrointestinal peristalsis, and promote digestive function, the row being beneficial to stool is rushed down, and has wide intestinal defecating feces excretion, therefore can remove body accumulation toxin, discharges intestinal parasite.Morning glory has removing water retention by purgation diuresis, effect by expectorant, detumescence, parasite killing anthelmintic, cures mainly the diseases such as limds edema, oedema due to nephritis, cirrhotic ascites, constipation, abdominal pain due to worm stagnation.The decoct of Spica Prunellae, water-leach liquor, alcohol-water leachate and alcohol leaching liquid all can obviously reduce laboratory animal blood pressure, and stem, leaf, fringe and herb all have hypotensive effect, but the effect of fringe is more obvious; The water decoction-alcohol sedimentation liquid mouse peritoneal injection of Spica Prunellae, has obvious antiinflammatory action; The decoct of Spica Prunellae all has certain inhibitory action to dysentery bacterium, Bacillus typhi, vibrio cholera, escherichia coli, Bacillus proteus, staphylococcus and Bacillus tuberculosis in vitro.Fructus Gleditsia has the effect of parasite killing, removing toxic substances: saponin element contained in Fructus Gleditsia is triterpene class and oligosaccharide, has antiinflammatory, antiulcer, disease-resistant change effect, also has anticancer and improves the effects such as acquired immune deficiency syndrome (AIDS) immunity.Rhizoma Atractylodis cure mainly drying damp and strengthening spleen; Wind-damp dispelling; Improving eyesight, main the spleen and stomach being stranded by dampness; Asthenia drowsiness; Born of the same parents' painful abdominal mass abdominal distention; Inappetence; Tell and have loose bowels; Phlegm retention; Damp-edema; Exterior syndrome folder is wet; Head heavy sensation of the body pain; Arthromyodynia warm nature; Podomere is ached and is weighed; Flaccidity folds in a garment; Nyctalopia.Herba Astragali Melilotoidis (Herba Astragali Sinici) cures mainly removing blood stasis, hemostasia and promoting granulation, and profit can support the five internal organs, improve visual and auditory acuity, hot blast of dispelling, makes us spry and light, and food is not minded for a long time, mends people, can stop hot malaria, flat stomach of invigorating blood circulation.Herba Houttuyniae has heat-clearing and toxic substances removing, eliminating carbuncle evacuation of pus, the effect of inducing diuresis for treating stranguria syndrome.Flos Lonicerae contains the pharmacological component such as chlorogenic acid, luteolin glycosides, stronger restraint is had to the various pathogens such as Hemolytic streptococcus, staphylococcus aureus and upper respiratory tract infection Causative virus etc., in addition also can enhancing immunity, antiearly pregnancy, hepatoprotective, antitumor, antiinflammatory, antipyretic, hemostasis (blood coagulation), suppress intestinal absorption cholesterol etc., its clinical application widely, can be used for the treatment of respiratory tract infection, bacillary dysentery, acute urinary, hypertension etc. more than 40 with other medicines compatibility and plant disease.Fructus Cnidii is the dry mature fruit of samphire cnidium monnieri, has resisting pathogenic microbes effect, and Fructus Cnidii all has inhibitory action to acrothesium floccosum, Gypsum Fibrosum sample microspore bacterium, ulotrichy microspore bacterium; Also there is antifungic action, Antifungal activities, insecticidal action.Galla Chinensis is cold in nature, sour in the mouth, puckery, has the effects such as pathogenic fire reducing of astringing the lung, relieving diarrhea with astringents, arresting sweating hemostasis, removing dampness sore, cures mainly the diseases such as chronic cough of deficiency lung, chronic diarrhea chronic dysentery, hemorrhoidal bleeding of having blood in stool, carbuncle sore tumefacting virus, Skin wet and rotten.The Fructus Sophorae is dry flower and alabastrum, the mature fruit of leguminous plant Chinese scholartree, energy clearing away heat-fire, cooling blood for hemostasis, and can be used for intestinal heat and have blood in stool, hemorrhoid swell hemorrhage, and liver-heat is had a headache, dizzy conjunctival congestion etc.The root of Radix Scutellariae is used as medicine, bitter in the mouth, cold in nature, there are heat clearing and damp drying, eliminating fire and detoxication, hemostasis, the effect such as antiabortive, cure mainly the diseases such as epidemic febrile disease, upper respiratory tract infection, cough due to lung-heat, damp and hot jaundice, pneumonia, dysentery, hemoptysis, conjunctival congestion, frequent fetal movement, hypertension, carbuncle furuncle, the clinical antibiotic property of Radix Scutellariae is better than Rhizoma Coptidis, and does not develop immunity to drugs.Bulbus Allii removing toxic substances parasite killing, reducing swelling and alleviating pain, antidiarrheal dysentery relieving, controls lung, anthelmintic, and also have warming the spleen warming the stomach in addition, control swollen ulcer drug, white bald tinea skin ulcer, dysentery is had loose bowels, pulmonary tuberculosis pertussis, ascarid pinworm, eating accumulation, coldness and pain in the epigastrium, edema distension.Herba Taraxaci energy bactericidal antiphlogistic, heat-clearing and toxic substances removing, diuresis eliminating stagnation, controls acute mastitis, lymphadenitis, scrofula, furuncle carbuncle toxin, acute conjunctivitis, cold, fever, acute tonsillitis, acute bronchitis, gastritis, hepatitis, cholecystitis, urinary tract infection.Rhizoma Zingiberis Recens has bactericidal effect, and can strengthen digestion power.Herba Sedi is the conventional medicinal herbs that folklore is extremely wide simply, and all herbal medicine, has effect of heat-clearing and toxic substances removing, swelling diuretic, evacuation of pus granulation promoting etc.Rhizoma Kaempferiae is the dry rhizome of zingiberaceous plant Rhizoma Kaempferiae, is excavated winter, and clean, remove fibrous root, section is dried, warming middle-JIAO for eliminating dampness, promoting the circulation of QI to relieve pain; Cure mainly acute gastroenteritis, dyspepsia, cold syndrome of the stomach pain, toothache, rheumatic arthritis, traumatic injury.Fructus Seu Herba Pubescentis all herbal medicine, controls the diseases such as dysfunctional uterine hemorrhage, cholecystitis, jaundice type hepatitis, headache due to common cold, stomachache, infantile malnutrition, acute catarrhal conjunctivitis, traumatic injury, treatment skin ulcer, sores ulceration of skin, Serpentis and lyssodexis, tinea pedis, the treatment of rotten head and hemorrhoid.Fructus Xanthii dispersing wind-cold, clearing the nasal passage, wind-damp dispelling, pain relieving.Bamboo salt is evaporated brine day to load in triennial QINGZHU, and two ends are sealed with natural loess, take pinaster as fuel, the material extract after 1000 DEG C ~ 1500 DEG C high-temperature calcinations, has antibacterial, antiinflammatory, toxopexic effect.Herba Kalimeridis is dry herb or the root of feverfew Herba Kalimeridis, and summer, autumn gather, using fresh herb or dry; Its acrid in the mouth, cool in nature, there is cooling blood for hemostasis, clearing away heat-damp and promoting diuresis, effect of removing toxic substances and promoting subsidence of swelling, can be used for spitting blood, epistaxis, dysentery, metrorrhagia, wound hemorrhage, jaundice, edema, stranguria with turbid discharge, flu, cough, pharyngalgia sore throat, hemorrhoid, carbuncle, erysipelas, infantile malnutrition.Winter honey refers to the Mel gathered in the crops in the winter time, has the effect of removing toxic substances, doctor's skin ulcer, pain relieving; Mel is directly embrocated on skin or wound, has the effect of antiinflammatory, pain relieving, stop blooding, alleviate edema, promotion wound healing; Eat and can alleviate alimentary toxicosis, treatment gastroenteritic ulcer and tripe abdomen pain; In addition, winter honey can coordinating the actions of various ingredients in a prescription, improves the property of medicine.Red line worm can be used as the high-quality animal feed of aquatic animal, can reduce the production cost of feedstuff, improves culture benefit and forms quality.Containing the multiple medicinal ingredient such as Pheretima element, Pheretima toxolysin, xanthine, antihistaminic and vitamin B in Lumbricus body, and to red sword, there is food calling effect.Fish oil can provide energy for red sword, and has food calling effect.
(2) Chinese herbal feed additive of the red swordfish white head and mouth fish disease of prevention of the present invention, it is the Chinese herbal medicine selected according to the mechanism of action of white head and mouth fish disease, and organically combined, there is significant cooperative synergism effect, play the function of each component to greatest extent, to the pathogen of red swordfish white head and mouth fish disease, there is good bactericidal and bacteriostatic effect.In addition, because the red line worm powder, Lumbricus powder and the fish oil that contain in Chinese herbal feed additive of the present invention have food calling effect, feedstuff mouthfeel can be improved to a certain extent, stimulate the taste bud of red swordfish, feedstuff of ingesting liked by red sword, therefore the composition that can strengthen red sword immunity and resistance, the pathogen of white head and mouth fish disease be had to remarkable bactericidal and bacteriostatic effect in additive of the present invention can enter in red sword body, and plays it and act on accordingly.
(3) in preparation method, crude drug purified water soaking a period of time, medical material deliquescing, cell expansion, effective ingredient is easily fried, and medical material contains starch, protein, after soaking, prevent when decocting, the starch gelatinization on medical material surface, protein coagulating, the capillary channel on blocking medical material surface, make the moisture when decocting enter not go, effective ingredient is not easily fried, affects curative effect of medication; Decoction can change the irritation effect of medicine, relatively mildly acts on red swordfish, promotes that red swordfish is to the absorption of medicine; Grinding makes the starch in medical material, protein is separated out more fully at subsequent step; Supercritical extraction, immersion, concentration step retain the effective ingredient in Chinese medicine of the present invention to greatest extent.
Detailed description of the invention
Following embodiment is convenient to understand the present invention better, but does not limit the present invention.Experimental technique in following embodiment, if no special instructions, is conventional method.
Embodiment 1
In the present invention's first detailed description of the invention, feed ingredient and preparation method thereof is as follows:
A kind of Chinese herbal feed additive preventing red swordfish white head and mouth fish disease, be made up of each component of following masses number: the infusion of Radix Sanguisorbae 30 parts, Herba Cirsii juice 25 parts, Folium camelliae assamicae (processed) 10 parts, Herba Sonchi Oleracei 23 parts, certain herbaceous plants with big flowers dish 23 parts, 19 parts, morning glory, Spica Prunellae 22 parts, 25 parts, Fructus Gleditsia, Rhizoma Atractylodis 20 parts, Herba Astragali Melilotoidis (Herba Astragali Sinici) 20 parts, Herba Houttuyniae 18 parts, Flos Lonicerae 20 parts, Fructus Cnidii 21 parts, Galla Chinensis 17 parts, the Fructus Sophorae 18 parts, Radix Scutellariae 19 parts, 15 parts, Bulbus Allii, Herba Taraxaci 14 parts, 13 parts, Rhizoma Zingiberis Recens, Herba Sedi 12 parts, Rhizoma Kaempferiae 12 parts, Fructus Seu Herba Pubescentis 10 parts, Fructus Xanthii 9 parts, bamboo salt 8 parts, Herba Kalimeridis juice 8 parts, winter honey 8 parts, 19 parts dry, red line worm powder, dry Lumbricus powder 17 parts, 17 parts, fish oil.
The preparation method of the Chinese herbal feed additive of above-mentioned prevention red swordfish white head and mouth fish disease is as follows:
(1) take Radix Sanguisorbae by proportioning, Radix Sanguisorbae is cleaned, drain the water, add the purified water that parts by weight are 4 times of Radix Sanguisorbae parts by weight, soak at room temperature 24-48h, filter to obtain the infusion of Radix Sanguisorbae;
(2) ground by Herba Cirsii or squeeze the juice with juice extractor, gained juice is Herba Cirsii juice, takes Herba Cirsii juice according to proportioning;
(3) take Folium camelliae assamicae (processed), Herba Sonchi Oleracei, certain herbaceous plants with big flowers dish, morning glory and Spica Prunellae by proportioning, the gross weight of Folium camelliae assamicae (processed), Herba Sonchi Oleracei, certain herbaceous plants with big flowers dish, morning glory and Spica Prunellae is denoted as 1 part, takes the purified water of 15 parts, at 70-80 DEG C, 2-3h is soaked in water-bath, obtains soak;
(4) boil 2h after the soak that step (3) obtains being heated to boiling, be separated and take out fried liquid, continue the purified water adding 6 parts in medical material, continue to boil 1.5h after being heated to boiling, merge twice fried liquid and be extracting solution;
(5) Fructus Gleditsia, Rhizoma Atractylodis, Herba Astragali Melilotoidis (Herba Astragali Sinici), Herba Houttuyniae, Flos Lonicerae, Fructus Cnidii, Galla Chinensis, the Fructus Sophorae, Radix Scutellariae, Bulbus Allii, Herba Taraxaci, Rhizoma Zingiberis Recens, Herba Sedi, Rhizoma Kaempferiae, Fructus Seu Herba Pubescentis and Fructus Xanthii is taken respectively by said ratio, load ball mill grinding 2h, cross 80 mesh sieves, obtain grounds travel;
(6) grounds travel that the extracting solution that obtains of step (4) and step (5) obtain is positioned in the extraction kettle in supercritical extraction instrument, carbon dioxide is utilized to extract as medium, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure 12Mpa, separator temperature is 70 DEG C, extracts 2 hours, is extracted liquid;
(7) bamboo salt, Herba Kalimeridis juice, winter honey, dry red line worm powder, dry Lumbricus powder and fish oil is taken according to proportioning, mix homogeneously with the infusion of the Radix Sanguisorbae of step (1) gained, the Herba Cirsii juice of step (2) gained, the extract of step (6) gained, obtain object.
The preparation method of described Herba Kalimeridis juice is: ground by Herba Kalimeridis or squeeze the juice with juice extractor, gained juice is Herba Kalimeridis juice.
The preparation method of the red line worm powder of described drying is: cleaned by red line worm fresh water, then uses distilled water flushing three times, grinds to form powdery after vacuum lyophilization to finish-drying, crosses 80 mesh sieves.
The preparation method of the Lumbricus powder of described drying is: cleaned by Lumbricus fresh water, then uses distilled water flushing three times, grinds to form powdery after vacuum lyophilization to finish-drying, crosses 80 mesh sieves.
Embodiment 2
In the present invention's second detailed description of the invention, feed ingredient and preparation method thereof is as follows:
A kind of Chinese herbal feed additive preventing red swordfish white head and mouth fish disease, be made up of each component of following masses number: the infusion of Radix Sanguisorbae 33 parts, Herba Cirsii juice 26 parts, Folium camelliae assamicae (processed) 14 parts, Herba Sonchi Oleracei 24 parts, certain herbaceous plants with big flowers dish 26 parts, 23 parts, morning glory, Spica Prunellae 25 parts, 26 parts, Fructus Gleditsia, Rhizoma Atractylodis 25 parts, Herba Astragali Melilotoidis (Herba Astragali Sinici) 23 parts, Herba Houttuyniae 22 parts, Flos Lonicerae 24 parts, Fructus Cnidii 23 parts, Galla Chinensis 22 parts, the Fructus Sophorae 24 parts, Radix Scutellariae 22 parts, 19 parts, Bulbus Allii, Herba Taraxaci 16 parts, 16 parts, Rhizoma Zingiberis Recens, Herba Sedi 15 parts, Rhizoma Kaempferiae 14 parts, Fructus Seu Herba Pubescentis 13 parts, Fructus Xanthii 11 parts, bamboo salt 10 parts, Herba Kalimeridis juice 11 parts, winter honey 11 parts, 25 parts dry, red line worm powder, dry Lumbricus powder 20 parts, 19 parts, fish oil.
The preparation method of the Chinese herbal feed additive of the red swordfish white head and mouth fish disease of prevention of the present embodiment is with embodiment 1.
Embodiment 3
In the present invention's the 3rd detailed description of the invention, feed ingredient and preparation method thereof is as follows:
A kind of Chinese herbal feed additive preventing red swordfish white head and mouth fish disease, be made up of each component of following masses number: the infusion of Radix Sanguisorbae 32 parts, Herba Cirsii juice 28 parts, Folium camelliae assamicae (processed) 13 parts, Herba Sonchi Oleracei 26 parts, certain herbaceous plants with big flowers dish 24 parts, 21 parts, morning glory, Spica Prunellae 23 parts, 28 parts, Fructus Gleditsia, Rhizoma Atractylodis 19 parts, Herba Astragali Melilotoidis (Herba Astragali Sinici) 21 parts, Herba Houttuyniae 19 parts, Flos Lonicerae 21 parts, Fructus Cnidii 24 parts, Galla Chinensis 19 parts, the Fructus Sophorae 20 parts, Radix Scutellariae 20 parts, 16 parts, Bulbus Allii, Herba Taraxaci 10 parts, 14 parts, Rhizoma Zingiberis Recens, Herba Sedi 13 parts, Rhizoma Kaempferiae 12 parts, Fructus Seu Herba Pubescentis 11 parts, Fructus Xanthii 10 parts, bamboo salt 9 parts, Herba Kalimeridis juice 9 parts, winter honey 9 parts, 18 parts dry, red line worm powder, dry Lumbricus powder 18 parts, 17 parts, fish oil.
The preparation method of the Chinese herbal feed additive of the red swordfish white head and mouth fish disease of prevention of the present embodiment is with embodiment 1.
Embodiment 4
In the present invention's the 4th detailed description of the invention, feed ingredient and preparation method thereof is as follows:
A kind of Chinese herbal feed additive preventing red swordfish white head and mouth fish disease, be made up of each component of following masses number: the infusion of Radix Sanguisorbae 30 parts, Herba Cirsii juice 24 parts, Folium camelliae assamicae (processed) 11 parts, Herba Sonchi Oleracei 22 parts, certain herbaceous plants with big flowers dish 22 parts, 18 parts, morning glory, Spica Prunellae 22 parts, 24 parts, Fructus Gleditsia, Rhizoma Atractylodis 22 parts, Herba Astragali Melilotoidis (Herba Astragali Sinici) 19 parts, Herba Houttuyniae 17 parts, Flos Lonicerae 19 parts, Fructus Cnidii 21 parts, Galla Chinensis 16 parts, the Fructus Sophorae 19 parts, Radix Scutellariae 17 parts, 14 parts, Bulbus Allii, Herba Taraxaci 14 parts, 12 parts, Rhizoma Zingiberis Recens, Herba Sedi 11 parts, Rhizoma Kaempferiae 11 parts, Fructus Seu Herba Pubescentis 9 parts, Fructus Xanthii 8 parts, bamboo salt 8 parts, Herba Kalimeridis juice 7 parts, winter honey 7 parts, 20 parts dry, red line worm powder, dry Lumbricus powder 16 parts, 17 parts, fish oil.
The preparation method of the Chinese herbal feed additive of the red swordfish white head and mouth fish disease of prevention of the present embodiment is with embodiment 1.
Embodiment 5
In the present invention's the 5th detailed description of the invention, feed ingredient and preparation method thereof is as follows:
A kind of Chinese herbal feed additive preventing red swordfish white head and mouth fish disease, be made up of each component of following masses number: the infusion of Radix Sanguisorbae 29 parts, Herba Cirsii juice 23 parts, Folium camelliae assamicae (processed) 9 parts, Herba Sonchi Oleracei 20 parts, certain herbaceous plants with big flowers dish 20 parts, 21 parts, morning glory, Spica Prunellae 19 parts, 21 parts, Fructus Gleditsia, Rhizoma Atractylodis 19 parts, Herba Astragali Melilotoidis (Herba Astragali Sinici) 18 parts, Herba Houttuyniae 16 parts, Flos Lonicerae 23 parts, Fructus Cnidii 20 parts, Galla Chinensis 18 parts, the Fructus Sophorae 18 parts, Radix Scutellariae 15 parts, 12 parts, Bulbus Allii, Herba Taraxaci 13 parts, 10 parts, Rhizoma Zingiberis Recens, Herba Sedi 15 parts, Rhizoma Kaempferiae 14 parts, Fructus Seu Herba Pubescentis 9 parts, Fructus Xanthii 11 parts, bamboo salt 7 parts, Herba Kalimeridis juice 10 parts, winter honey 10 parts, 20 parts dry, red line worm powder, dry Lumbricus powder 14 parts, 16 parts, fish oil.
The preparation method of the Chinese herbal feed additive of the red swordfish white head and mouth fish disease of prevention of the present embodiment is with embodiment 1.
Embodiment 6
In the present invention's the 6th detailed description of the invention, feed ingredient and preparation method thereof is as follows:
A kind of Chinese herbal feed additive preventing red swordfish white head and mouth fish disease, be made up of each component of following masses number: the infusion of Radix Sanguisorbae 25 parts, Herba Cirsii juice 22 parts, Folium camelliae assamicae (processed) 7 parts, Herba Sonchi Oleracei 20 parts, certain herbaceous plants with big flowers dish 19 parts, 15 parts, morning glory, Spica Prunellae 19 parts, 21 parts, Fructus Gleditsia, Rhizoma Atractylodis 17 parts, Herba Astragali Melilotoidis (Herba Astragali Sinici) 17 parts, Herba Houttuyniae 13 parts, Flos Lonicerae 16 parts, Fructus Cnidii 16 parts, Galla Chinensis 14 parts, the Fructus Sophorae 15 parts, Radix Scutellariae 15 parts, 11 parts, Bulbus Allii, Herba Taraxaci 12 parts, 9 parts, Rhizoma Zingiberis Recens, Herba Sedi 8 parts, Rhizoma Kaempferiae 9 parts, Fructus Seu Herba Pubescentis 7 parts, Fructus Xanthii 6 parts, bamboo salt 6 parts, Herba Kalimeridis juice 5 parts, winter honey 5 parts, 16 parts dry, red line worm powder, dry Lumbricus powder 18 parts, 14 parts, fish oil.
The preparation method of the Chinese herbal feed additive of the red swordfish white head and mouth fish disease of prevention of the present embodiment is with embodiment 1.
Experimental result
Preventive effect is tested
With normal feedstuff group of throwing something and feeding (for normal commercial diet) for matched group, to add the feedstuff group of the embodiment of the present invention 1 in normal feedstuff for experimental group, often group establish 3 parallel, in experimental group, the additive of embodiment 1 and the weight ratio of normal feedstuff are 1:20.Choose 6 aquariums, healthy 60, red sword, red for health sword is all assigned to 6 aquariums at random, each aquarium 10 red swordfishes, in each aquarium, add the red swordfish that 1 is suffered from white head and mouth fish disease.The results are shown in Table 1:
The effect situation of table 1 Chinese herbal feed additive prevention of the present invention white head and mouth fish disease
From table 1, the Chinese herbal feed additive of prevention of the present invention red swordfish white head and mouth fish disease effectively can reduce the incidence rate of red swordfish white head and mouth fish disease.
Bacteriostatic test checks
Adopt nitrofural to do matched group, the Chinese herbal feed additive of the prevention red swordfish white head and mouth fish disease of the embodiment of the present invention 1 is that experimental group carries out antibacterial inspection.
The preparation of culture medium: take in the water-soluble 800mL of beef extract 5g, adds peptone 10g and dissolves, and adds casein 34g and dissolves, then adds NaCl5g dissolving, is adjusted to pH7.6 ~ 7.8, is diluted to 1000mL.Add agar powder 18g, autoclaving 15min, when being cooled to 40 DEG C ~ 50 DEG C, a kind of rhyme scheme in Chinese operas serving as the prelude to a complete score for voices, solidifies, and the 4 DEG C of refrigerators placing no bacteria pollution are for subsequent use.
Strain is: myxobacter.
Matched group antibacterial liquid is prepared: be dissolved in respectively in 10ml sterile deionized water by 1 part of nitrofural, diluted respectively by liquor strength, with stock solution than being 1:2,1:4,1:8,1:16,1:32.
Experimental group antibacterial liquid is prepared: be dissolved in 10ml sterile deionized water by the Chinese herbal feed additive of red for the prevention of the embodiment of the present invention 1 swordfish white head and mouth fish disease, diluted respectively by liquor strength, with stock solution than being 1:2,1:4,1:8,1:16,1:32.To the fungistatic effect of myxobacter in table 2:
The bactericidal and bacteriostatic effect of table 2 Chinese herbal feed additive of the present invention
From table 2, the Chinese herbal feed additive of the prevention red swordfish white head and mouth fish disease of embodiments of the invention 1 has good bactericidal and bacteriostatic effect.
The above is only the preferred embodiment of the present invention, is noted that for those skilled in the art, and under the premise without departing from the principles of the invention, can also make some improvement, these improvement should be considered as protection scope of the present invention.
Claims (9)
1. the Chinese herbal feed additive of the red swordfish white head and mouth fish disease of prevention, it is characterized in that: be made up of each component of following masses number: infusion 25-33 part of Radix Sanguisorbae, Herba Cirsii juice 22-28 part, Folium camelliae assamicae (processed) 7-14 part, Herba Sonchi Oleracei 20-26 part, certain herbaceous plants with big flowers dish 19-26 part, morning glory 15-23 part, Spica Prunellae 19-25 part, Fructus Gleditsia 21-28 part, Rhizoma Atractylodis 17-25 part, Herba Astragali Melilotoidis (Herba Astragali Sinici) 17-23 part, Herba Houttuyniae 13-22 part, Flos Lonicerae 16-24 part, Fructus Cnidii 16-24 part, Galla Chinensis 14-22 part, Fructus Sophorae 15-24 part, Radix Scutellariae 15-22 part, Bulbus Allii 11-19 part, Herba Taraxaci 10-16 part, Rhizoma Zingiberis Recens 9-16 part, Herba Sedi 8-15 part, Rhizoma Kaempferiae 9-14 part, Fructus Seu Herba Pubescentis 7-13 part, Fructus Xanthii 6-11 part, bamboo salt 6-10 part, Herba Kalimeridis juice 5-11 part, winter honey 5-11 part, dry red line worm powder 16-25 part, dry Lumbricus powder 14-20 part, fish oil 14-19 part, described Herba Kalimeridis juice is identical with the mass fraction of winter honey.
2. the Chinese herbal feed additive of the red swordfish white head and mouth fish disease of prevention according to claim 1, it is characterized in that: the Chinese herbal feed additive of the red swordfish white head and mouth fish disease of described prevention, is made up of each component of following masses number: infusion 29-30 part of Radix Sanguisorbae, Herba Cirsii juice 24-26 part, Folium camelliae assamicae (processed) 9-11 part, Herba Sonchi Oleracei 22-24 part, certain herbaceous plants with big flowers dish 22-24 part, morning glory 18-21 part, Spica Prunellae 22-23 part, Fructus Gleditsia 24-26 part, Rhizoma Atractylodis 19-22 part, Herba Astragali Melilotoidis (Herba Astragali Sinici) 19-21 part, Herba Houttuyniae 17-19 part, Flos Lonicerae 19-21 part, Fructus Cnidii 20-21 part, Galla Chinensis 16-19 part, Fructus Sophorae 18-19 part, Radix Scutellariae 17-20 part, Bulbus Allii 14-16 part, Herba Taraxaci 13-14 part, Rhizoma Zingiberis Recens 12-14 part, Herba Sedi 11-13 part, Rhizoma Kaempferiae 11-12 part, Fructus Seu Herba Pubescentis 9-11 part, Fructus Xanthii 8-10 part, bamboo salt 7-9 part, Herba Kalimeridis juice 7-9 part, winter honey 7-9 part, dry red line worm powder 18-20 part, dry Lumbricus powder 16-18 part, fish oil 16-17 part, described Herba Kalimeridis juice is identical with the mass fraction of winter honey.
3. the Chinese herbal feed additive of the red swordfish white head and mouth fish disease of prevention according to claim 1, it is characterized in that: the Chinese herbal feed additive of the red swordfish white head and mouth fish disease of described prevention, be made up of each component of following masses number: the infusion of Radix Sanguisorbae 30 parts, Herba Cirsii juice 25 parts, Folium camelliae assamicae (processed) 10 parts, Herba Sonchi Oleracei 23 parts, certain herbaceous plants with big flowers dish 23 parts, 19 parts, morning glory, Spica Prunellae 22 parts, 25 parts, Fructus Gleditsia, Rhizoma Atractylodis 20 parts, Herba Astragali Melilotoidis (Herba Astragali Sinici) 20 parts, Herba Houttuyniae 18 parts, Flos Lonicerae 20 parts, Fructus Cnidii 21 parts, Galla Chinensis 17 parts, the Fructus Sophorae 18 parts, Radix Scutellariae 19 parts, 15 parts, Bulbus Allii, Herba Taraxaci 14 parts, 13 parts, Rhizoma Zingiberis Recens, Herba Sedi 12 parts, Rhizoma Kaempferiae 12 parts, Fructus Seu Herba Pubescentis 10 parts, Fructus Xanthii 9 parts, bamboo salt 8 parts, Herba Kalimeridis juice 8 parts, winter honey 8 parts, 19 parts dry, red line worm powder, dry Lumbricus powder 17 parts, 17 parts, fish oil.
4. a preparation method for the Chinese herbal feed additive of the red swordfish white head and mouth fish disease of the prevention according to any one of claim 1-3, is characterized in that: comprise the following steps:
(1) take Radix Sanguisorbae by proportioning, Radix Sanguisorbae is cleaned, drain the water, add the purified water that parts by weight are 4 times of Radix Sanguisorbae parts by weight, soak at room temperature 24-48h, filter to obtain the infusion of Radix Sanguisorbae;
(2) ground by Herba Cirsii or squeeze the juice with juice extractor, gained juice is Herba Cirsii juice, takes Herba Cirsii juice according to proportioning;
(3) take Folium camelliae assamicae (processed), Herba Sonchi Oleracei, certain herbaceous plants with big flowers dish, morning glory and Spica Prunellae by proportioning, the gross weight of Folium camelliae assamicae (processed), Herba Sonchi Oleracei, certain herbaceous plants with big flowers dish, morning glory and Spica Prunellae is denoted as 1 part, takes the purified water of 15 parts, at 70-80 DEG C, 2-3h is soaked in water-bath, obtains soak;
(4) boil 2h after the soak that step (3) obtains being heated to boiling, be separated and take out fried liquid, continue the purified water adding 6 parts in medical material, continue to boil 1.5h after being heated to boiling, merge twice fried liquid and be extracting solution;
(5) Fructus Gleditsia, Rhizoma Atractylodis, Herba Astragali Melilotoidis (Herba Astragali Sinici), Herba Houttuyniae, Flos Lonicerae, Fructus Cnidii, Galla Chinensis, the Fructus Sophorae, Radix Scutellariae, Bulbus Allii, Herba Taraxaci, Rhizoma Zingiberis Recens, Herba Sedi, Rhizoma Kaempferiae, Fructus Seu Herba Pubescentis and Fructus Xanthii is taken respectively by said ratio, load ball mill grinding 2h, cross 80 mesh sieves, obtain grounds travel;
(6) grounds travel that the extracting solution that obtains of step (4) and step (5) obtain is positioned in the extraction kettle in supercritical extraction instrument, carbon dioxide is utilized to extract as medium, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure 12Mpa, separator temperature is 70 DEG C, extracts 2 hours, is extracted liquid;
(7) bamboo salt, Herba Kalimeridis juice, winter honey, dry red line worm powder, dry Lumbricus powder and fish oil is taken according to proportioning, mix homogeneously with the infusion of the Radix Sanguisorbae of step (1) gained, the Herba Cirsii juice of step (2) gained, the extract of step (6) gained, obtain object.
5. the preparation method of the Chinese herbal feed additive of the red swordfish white head and mouth fish disease of prevention according to claim 4, is characterized in that: the preparation method of described Herba Kalimeridis juice is: ground by Herba Kalimeridis or squeeze the juice with juice extractor, gained juice is Herba Kalimeridis juice.
6. the preparation method of the Chinese herbal feed additive of the red swordfish white head and mouth fish disease of prevention according to claim 4, it is characterized in that: the preparation method of the red line worm powder of described drying is: cleaned by red line worm fresh water, use distilled water flushing again three times, grind to form powdery after vacuum lyophilization to finish-drying, cross 80 mesh sieves.
7. the preparation method of the Chinese herbal feed additive of the red swordfish white head and mouth fish disease of prevention according to claim 4, it is characterized in that: the preparation method of the Lumbricus powder of described drying is: cleaned by Lumbricus fresh water, use distilled water flushing again three times, grind to form powdery after vacuum lyophilization to finish-drying, cross 80 mesh sieves.
8. a using method for the Chinese herbal feed additive of the red swordfish white head and mouth fish disease of the prevention according to any one of claim 1-3, is characterized in that: throw something and feed after being mixed homogeneously with the weight ratio of 1:30-1:10 with commercially available normal feedstuff by the Chinese herbal feed additive of red for prevention of the present invention swordfish white head and mouth fish disease.
9. a using method for the Chinese herbal feed additive of the red swordfish white head and mouth fish disease of prevention according to claim 8, is characterized in that: the Chinese herbal feed additive of prevention of the present invention red swordfish white head and mouth fish disease and the weight ratio of commercially available normal feedstuff are 1:20.
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Application publication date: 20151202 |