CN104838762B - A kind of method for promoting tsaoko seed to sprout - Google Patents

A kind of method for promoting tsaoko seed to sprout Download PDF

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CN104838762B
CN104838762B CN201510261360.1A CN201510261360A CN104838762B CN 104838762 B CN104838762 B CN 104838762B CN 201510261360 A CN201510261360 A CN 201510261360A CN 104838762 B CN104838762 B CN 104838762B
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seed
tsaoko
perlite
weight
parts
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CN104838762A (en
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潘春柳
邓志军
黄燕芬
韦坤华
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Guangxi Botanical Garden of Medicinal Plants
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Abstract

The present invention relates to a kind of method for promoting tsaoko seed to sprout, it is to be sufficiently mixed tsaoko seed for 1: 3 by volume with wet perlite when carrying out Stratificated treatment, lamination 90 100 days at 10 15 DEG C are placed in after sealing, the wet perlite is well mixed and obtained by the perlite of 1 parts by weight and the water of 1.4 2 parts by weight, the tsaoko seed after Stratificated treatment is sown into again and sprouted in matrix, alternate culture 28 days under two conditions, the first condition is to handle 12h under 30 DEG C of illumination, second of condition is to handle 12h under 20 DEG C of dark, the matrix of sprouting is well mixed and is made by the perlite of 1 parts by weight and the water of 2.5 3 parts by weight.The method provided by the invention for promoting tsaoko seed to sprout, germination rate is high, and it is simple to operate, cost is low, is easy for workers to operate, there is very high application value to tsaoko seed seedling-raising, large-scale planting.

Description

A kind of method for promoting tsaoko seed to sprout
Technical field
The invention belongs to field of plant growing technology, and in particular to a kind of method for promoting tsaoko seed to sprout.
Background technology
Tsaoko (Amomum tsaoko Crevost et Lemarie) is under the jurisdiction of Zingiber (Zingiberaceae) Amomum (Amomum) provinces and regions such as Yunnan, Guangxi, Guizhou, are originated in.Be used as medicine with dry mature fruit, there is eliminating dampness middle benefit gas, preventing malaria eliminating phlegm it Effect, for internal retention of cold wetness, abdominal distention, feeling of fullness vomiting, malaria fever and chills and pestilence heating.In addition, tsaoko can also make flavoring, Complete stool can extract aromatic oil.In production, tsaoko is unable to dry storage based on seminal propagation after tsaoko seed collection, shines Easily lost vigor after dry, and the tsaoko seed of freshly harvested is in a dormant state, directly sowing germination is slow, and germination rate is low, goes out Seedling is irregular.Now there are some researches show GA3、H2SO4, NaOH or wet sand such as hide at the processing can promote the sprouting of tsaoko seed, but imitate Fruit is bad, highest germination rate only 50% or so.
The content of the invention
The purpose of the present invention is in view of the shortcomings of the prior art, there is provided a kind of method for promoting tsaoko seed to sprout.
A kind of method for promoting tsaoko seed to sprout provided by the present invention, comprises the following steps:
Tsaoko seed is sufficiently mixed for 1: 3 by volume when carrying out Stratificated treatment with wet perlite, is placed in after sealing Lamination 90-100 days at 10-15 DEG C, the wet perlite are well mixed by the perlite of 1 parts by weight and the water of 1.4-2 parts by weight Obtain.
Preferably, the described method for promoting the sprouting of tsaoko seed, sprouting is sown into by the tsaoko seed after Stratificated treatment In matrix, alternate culture 28 days, the first condition are that 12h is handled under 30 DEG C of illumination under two conditions, and second of condition is 20 Handle 12h under DEG C dark, it is described to sprout matrix by the perlite of 1 parts by weight and the water of 2.5-3 parts by weight is well mixed is made.
Preferably, the described method for promoting tsaoko seed to sprout, the perlite and water by 121 DEG C, 20min it is high Warm sterilization treatment.
Preferably, the described method for promoting the sprouting of tsaoko seed, carries out seed disinfection before the Stratificated treatment, Tsaoko seed is put into 0.1% liquor potassic permanganate and soaks 25-30min, is then rinsed well with clear water, it is disposed within logical At wind, tsaoko the surface of the seed moisture is dried.
Preferably, the described method for promoting the sprouting of tsaoko seed, carries out seed treatment before the seed disinfection, The tsaoko fruit of fresh mature no disease and pests harm is gathered, the tsaoko fruit is placed in into room temperature in ventilative Polywoven Bag stacks 2- 3 days, outside rind is divested, obtains seed group, the seed group is placed in the container of cleaning and stacks 12-18 hours, then will kind Son group is put into clear water and washed by rubbing with the hands, removes pulp and aril, rejects impurity and flat grain, obtains complete full tsaoko seed.
Preferably, the described method for promoting the sprouting of tsaoko seed, daily to plastics in tsaoko fruit stacking process Watered 3 times in bag.
Preferably, the described method for promoting tsaoko seed to sprout, it is saturating every 7 days depressurizations in the Stratificated treatment Gas 1 time simultaneously adds 0.05-0.5 parts by weight water.
The present invention comprises at least following beneficial effect:
Firstth, tsaoko seed is mixed into wet perlite and carries out Stratificated treatment, can because of the water conservation of perlite, good permeability Prevent seed from being rotted during lamination, released the dormancy of seed, the vigor of tsaoko seed has been effectively ensured, promote kind The sprouting of son.
Secondth, using the light dark alternating vernalization that relents, the vigor of enzyme in tsaoko seed can be effectively improved, promotes nutrition Material converts, and improves germination percentage.
3rd, after divesting tsaoko fruit outside rind, seed group is placed in the container of cleaning and stacks 12-18 hours, heap Due to the effect of enzyme, bacterium and temperature etc. during putting, seed group pulp softening, seed group is put into clear water again after stacking and rubbed with the hands Outer layer aril and pulp can be removed by washing, and be washed by rubbing with the hands with existing using sand and plant ash, and the seed impurity that this method obtains is few, Totally, it is easy to the later stage to choose seeds.
4th, tsaoko seed is carried out disinfection, perlite and water carry out high-temperature sterilization processing, can effectively reduce seed in layer Going mouldy in product or pregermination procedure.
5th, the method provided by the invention for promoting tsaoko seed to sprout, germination rate is and simple to operate more than 80%, Cost is low, is easy for workers to operate, and tsaoko seed seedling-raising, large-scale planting are of very high actual application value.
Other advantages, target and the feature of the present invention embodies part by following explanation, and part will also be by this The research and practice of invention and be understood by the person skilled in the art.
Embodiment
With reference to embodiment, the present invention is described in further detail, to make those skilled in the art with reference to specification Word can be implemented according to this.
Experimental method described in following embodiments, it is conventional method unless otherwise specified, the reagent and material, such as Without specified otherwise, commercially obtain.
Embodiment 1:
A kind of method for promoting tsaoko seed to sprout, comprises the following steps:
Tsaoko seed is sufficiently mixed for 1: 3 by volume when carrying out Stratificated treatment with wet perlite, is placed in after sealing Lamination 100 days at 10 DEG C, the wet perlite are well mixed and obtained by the perlite of 1 parts by weight and the water of 1.4 parts by weight.By grass Fruit seed, which is mixed into wet perlite, carries out Stratificated treatment, because of perlite water conservation, good permeability, can prevent seed during lamination Rot, released the dormancy of seed, the vigor of tsaoko seed has been effectively ensured, promote the sprouting of seed.
Described promotes the method that tsaoko seed is sprouted, and the tsaoko seed after Stratificated treatment is sown into and sprouted in matrix, Alternate culture 28 days under the conditions of two kinds, the first condition are that 12h is handled under 30 DEG C of illumination, and second of condition is to locate under 20 DEG C of dark 12h is managed, the matrix of sprouting is well mixed and is made by the perlite of 1 parts by weight and the water of 2.5 parts by weight.Using the illumination that relents Dark alternately vernalization, the vigor of enzyme in tsaoko seed can be effectively improved, promote nutriment conversion, improve germination percentage.
The described method for promoting tsaoko seed to sprout, the perlite and water by 121 DEG C, 20min high-temperature sterilizations at Reason.
Described promotes the method that tsaoko seed is sprouted, and seed disinfection is carried out before the Stratificated treatment, by tsaoko kind Son, which is put into 0.1% liquor potassic permanganate, soaks 25min, is then rinsed well with clear water, ventilation disposed within, dries grass Fruit the surface of the seed moisture.Tsaoko seed is carried out disinfection, can effectively reduce seed going mouldy in lamination or pregermination procedure.
Described promotes the method that tsaoko seed is sprouted, and seed treatment is carried out before the seed disinfection, and collection is fresh The tsaoko fruit of ripe no disease and pests harm, the tsaoko fruit is placed in ventilative Polywoven Bag room temperature and stacks 2 days, daily to Watered 3 times in polybag, divest outside rind, obtain seed group, the seed group is placed in the container of cleaning and stacked 12 hours, Then seed group is put into clear water to wash by rubbing with the hands, removes pulp and aril, reject impurity and flat grain, obtain complete full tsaoko kind Son.After divesting tsaoko fruit outside rind, seed group is placed in the container of cleaning and stacked, due to enzyme, bacterium and temperature etc. Effect, seed group pulp softening, outer layer aril and pulp can be removed by being put into clear water to wash by rubbing with the hands by seed group after stacking, with showing Some is washed by rubbing with the hands using sand and plant ash, and the seed impurity that this method obtains is few, totally, is easy to the later stage to choose seeds.
Described promotes the method that tsaoko seed is sprouted, and breathes freely 1 time and mends every 7 days depressurizations in the Stratificated treatment Add 0.05 parts by weight water.
Embodiment 2:
Tsaoko seed is sufficiently mixed for 1: 3 by volume when carrying out Stratificated treatment with wet perlite, is placed in after sealing Lamination 95 days at 12.5 DEG C, the wet perlite are well mixed and obtained by the perlite of 1 parts by weight and the water of 1.7 parts by weight.Will Tsaoko seed, which is mixed into wet perlite, carries out Stratificated treatment, because of perlite water conservation, good permeability, seed can be prevented in lamination process In rot, released the dormancy of seed, the vigor of tsaoko seed be effectively ensured, promote the sprouting of seed.
Described promotes the method that tsaoko seed is sprouted, and the tsaoko seed after Stratificated treatment is sown into and sprouted in matrix, Alternate culture 28 days under the conditions of two kinds, the first condition are that 12h is handled under 30 DEG C of illumination, and second of condition is to locate under 20 DEG C of dark 12h is managed, the matrix of sprouting is well mixed and is made by the perlite of 1 parts by weight and the water of 2.8 parts by weight.Using the illumination that relents Dark alternately vernalization, the vigor of enzyme in tsaoko seed can be effectively improved, promote nutriment conversion, improve germination percentage.
The described method for promoting tsaoko seed to sprout, the perlite and water by 121 DEG C, 20min high-temperature sterilizations at Reason.
Described promotes the method that tsaoko seed is sprouted, and seed disinfection is carried out before the Stratificated treatment, by tsaoko kind Son, which is put into 0.1% liquor potassic permanganate, soaks 28min, is then rinsed well with clear water, ventilation disposed within, dries grass Fruit the surface of the seed moisture.Tsaoko seed is carried out disinfection, can effectively reduce seed going mouldy in lamination or pregermination procedure.
Described promotes the method that tsaoko seed is sprouted, and seed treatment is carried out before the seed disinfection, and collection is fresh The tsaoko fruit of ripe no disease and pests harm, the tsaoko fruit is placed in ventilative Polywoven Bag room temperature and stacks 2 days, daily to Watered 3 times in polybag, divest outside rind, obtain seed group, the seed group is placed in the container of cleaning and stacked 15 hours, Then seed group is put into clear water to wash by rubbing with the hands, removes pulp and aril, reject impurity and flat grain, obtain complete full tsaoko kind Son.After divesting tsaoko fruit outside rind, seed group is placed in the container of cleaning and stacked, due to enzyme, bacterium and temperature etc. Effect, seed group pulp softening, outer layer aril and pulp can be removed by being put into clear water to wash by rubbing with the hands by seed group after stacking, with showing Some is washed by rubbing with the hands using sand and plant ash, and the seed impurity that this method obtains is few, totally, is easy to the later stage to choose seeds.
Described promotes the method that tsaoko seed is sprouted, and breathes freely 1 time and mends every 7 days depressurizations in the Stratificated treatment Add 0.1 parts by weight water.
Embodiment 3:
Tsaoko seed is sufficiently mixed for 1: 3 by volume when carrying out Stratificated treatment with wet perlite, is placed in after sealing Lamination 90 days at 15 DEG C, the wet perlite are well mixed and obtained by the perlite of 1 parts by weight and the water of 2 parts by weight.By tsaoko Seed, which is mixed into wet perlite, carries out Stratificated treatment, because of perlite water conservation, good permeability, can prevent that seed is rotten during lamination It is rotten, the dormancy of seed is released, the vigor of tsaoko seed has been effectively ensured, promotes the sprouting of seed.
Described promotes the method that tsaoko seed is sprouted, and the tsaoko seed after Stratificated treatment is sown into and sprouted in matrix, Alternate culture 28 days under the conditions of two kinds, the first condition are that 12h is handled under 30 DEG C of illumination, and second of condition is to locate under 20 DEG C of dark 12h is managed, the matrix of sprouting is well mixed and is made by the perlite of 1 parts by weight and the water of 3 parts by weight.It is black using the illumination that relents Dark alternately vernalization, the vigor of enzyme in tsaoko seed can be effectively improved, promote nutriment conversion, improve germination percentage.
The described method for promoting tsaoko seed to sprout, the perlite and water by 121 DEG C, 20min high-temperature sterilizations at Reason.
Described promotes the method that tsaoko seed is sprouted, and seed disinfection is carried out before the Stratificated treatment, by tsaoko kind Son, which is put into 0.1% liquor potassic permanganate, soaks 30min, is then rinsed well with clear water, ventilation disposed within, dries grass Fruit the surface of the seed moisture.Tsaoko seed is carried out disinfection, can effectively reduce seed going mouldy in lamination or pregermination procedure.
Described promotes the method that tsaoko seed is sprouted, and seed treatment is carried out before the seed disinfection, and collection is fresh The tsaoko fruit of ripe no disease and pests harm, the tsaoko fruit is placed in ventilative Polywoven Bag room temperature and stacks 3 days, daily to Watered 3 times in polybag, divest outside rind, obtain seed group, the seed group is placed in the container of cleaning and stacked 18 hours, Then seed group is put into clear water to wash by rubbing with the hands, removes pulp and aril, reject impurity and flat grain, obtain complete full tsaoko kind Son.After divesting tsaoko fruit outside rind, seed group is placed in the container of cleaning and stacked, due to enzyme, bacterium and temperature etc. Effect, seed group pulp softening, outer layer aril and pulp can be removed by being put into clear water to wash by rubbing with the hands by seed group after stacking, with showing Some is washed by rubbing with the hands using sand and plant ash, and the seed impurity that this method obtains is few, totally, is easy to the later stage to choose seeds.
Described promotes the method that tsaoko seed is sprouted, and breathes freely 1 time and mends every 7 days depressurizations in the Stratificated treatment Add 0.2 parts by weight water.
Embodiment 4:
Tsaoko seed is sufficiently mixed for 1: 3 by volume when carrying out Stratificated treatment with wet perlite, is placed in after sealing Lamination 100 days at 15 DEG C, the wet perlite are well mixed and obtained by the perlite of 1 parts by weight and the water of 2 parts by weight.By tsaoko Seed, which is mixed into wet perlite, carries out Stratificated treatment, because of perlite water conservation, good permeability, can prevent that seed is rotten during lamination It is rotten, the dormancy of seed is released, the vigor of tsaoko seed has been effectively ensured, promotes the sprouting of seed.
Described promotes the method that tsaoko seed is sprouted, and the tsaoko seed after Stratificated treatment is sown into and sprouted in matrix, Alternate culture 28 days under the conditions of two kinds, the first condition are that 12h is handled under 30 DEG C of illumination, and second of condition is to locate under 20 DEG C of dark 12h is managed, the matrix of sprouting is well mixed and is made by the perlite of 1 parts by weight and the water of 3 parts by weight.It is black using the illumination that relents Dark alternately vernalization, the vigor of enzyme in tsaoko seed can be effectively improved, promote nutriment conversion, improve germination percentage.
The described method for promoting tsaoko seed to sprout, the perlite and water by 121 DEG C, 20min high-temperature sterilizations at Reason.
Described promotes the method that tsaoko seed is sprouted, and seed disinfection is carried out before the Stratificated treatment, by tsaoko kind Son, which is put into 0.1% liquor potassic permanganate, soaks 30min, is then rinsed well with clear water, ventilation disposed within, dries grass Fruit the surface of the seed moisture.Tsaoko seed is carried out disinfection, can effectively reduce seed going mouldy in lamination or pregermination procedure.
Described promotes the method that tsaoko seed is sprouted, and seed treatment is carried out before the seed disinfection, and collection is fresh The tsaoko fruit of ripe no disease and pests harm, the tsaoko fruit is placed in ventilative Polywoven Bag room temperature and stacks 3 days, daily to Watered 3 times in polybag, divest outside rind, obtain seed group, the seed group is placed in the container of cleaning and stacked 18 hours, Then seed group is put into clear water to wash by rubbing with the hands, removes pulp and aril, reject impurity and flat grain, obtain complete full tsaoko kind Son.After divesting tsaoko fruit outside rind, seed group is placed in the container of cleaning and stacked, due to enzyme, bacterium and temperature etc. Effect, seed group pulp softening, outer layer aril and pulp can be removed by being put into clear water to wash by rubbing with the hands by seed group after stacking, with showing Some is washed by rubbing with the hands using sand and plant ash, and the seed impurity that this method obtains is few, totally, is easy to the later stage to choose seeds.
Described promotes the method that tsaoko seed is sprouted, and breathes freely 1 time and mends every 7 days depressurizations in the Stratificated treatment Add 0.5 parts by weight water.
Embodiment 5:
Tsaoko seed is sufficiently mixed for 1: 3 by volume when carrying out Stratificated treatment with wet perlite, is placed in after sealing Lamination 100 days at 15 DEG C, the wet perlite are well mixed and obtained by the perlite of 1 parts by weight and the water of 2 parts by weight.By tsaoko Seed, which is mixed into wet perlite, carries out Stratificated treatment, because of perlite water conservation, good permeability, can prevent that seed is rotten during lamination It is rotten, the dormancy of seed is released, the vigor of tsaoko seed has been effectively ensured, promotes the sprouting of seed.
Described promotes the method that tsaoko seed is sprouted, and the tsaoko seed after Stratificated treatment is sown into and sprouted in matrix, Alternate culture 28 days under the conditions of two kinds, the first condition are that 12h is handled under 30 DEG C of illumination, and second of condition is to locate under 20 DEG C of dark 12h is managed, the matrix of sprouting is well mixed and is made by the perlite of 1 parts by weight and the water of 3 parts by weight.It is black using the illumination that relents Dark alternately vernalization, the vigor of enzyme in tsaoko seed can be effectively improved, promote nutriment conversion, improve germination percentage.
Described promotes the method that tsaoko seed is sprouted, and seed treatment is carried out before the Stratificated treatment, and collection is fresh The tsaoko fruit of ripe no disease and pests harm, the tsaoko fruit is placed in ventilative Polywoven Bag room temperature and stacks 3 days, daily to Watered 3 times in polybag, divest outside rind, obtain seed group, the seed group is placed in the container of cleaning and stacked 18 hours, Then seed group is put into clear water to wash by rubbing with the hands, removes pulp and aril, reject impurity and flat grain, obtain complete full tsaoko kind Son.After divesting tsaoko fruit outside rind, seed group is placed in the container of cleaning and stacked, due to enzyme, bacterium and temperature etc. Effect, seed group pulp softening, outer layer aril and pulp can be removed by being put into clear water to wash by rubbing with the hands by seed group after stacking, with showing Some is washed by rubbing with the hands using sand and plant ash, and the seed impurity that this method obtains is few, totally, is easy to the later stage to choose seeds.
Described promotes the method that tsaoko seed is sprouted, and breathes freely 1 time and mends every 7 days depressurizations in the Stratificated treatment Add 0.5 parts by weight water.
Comparative example 1:
Beneficial effects of the present invention are further illustrated by comparative example.Wherein, common sprouting method is directly by new fresh grass Fruit seed, which is sown into wet sand, to be sprouted;It is that fresh tsaoko seed is sown into perlite to sprout that perlite, which sprouts method,;Wet sand bed area method It is on the basis of embodiment 5, uses Stratificated treatment instead common wet sand lamination.
Influence of the 1 different germination methods of table to tsaoko seed germination rate
As shown in Table 1, the method that tsaoko seed of the present invention is sprouted, compared with common sprouting method, germination rate improves 78-84%, 47-53% is improved compared with perlite sprouts method, compared with wet sand bed area method germination rate improves 39.5- 45.5%.
Although embodiment of the present invention is disclosed as above, it is not restricted in specification and embodiment listed With.It can be applied to various suitable the field of the invention completely., can be easily for those skilled in the art Realize other modification.Therefore it is of the invention and unlimited under the universal limited without departing substantially from claim and equivalency range In specific details and shown here as the example with description.

Claims (2)

  1. A kind of 1. method for promoting tsaoko seed to sprout, it is characterised in that be by tsaoko seed when carrying out Stratificated treatment with it is wet Perlite is 1 by volume:3 are sufficiently mixed, and lamination 90-100 days at 10-15 DEG C are placed in after sealing, the wet perlite is by 1 weight Measure the perlite of part and the water of 1.4-2 parts by weight is well mixed and obtained;
    Tsaoko seed after Stratificated treatment is sown into and sprouted in matrix, under two conditions alternate culture 28 days, the first condition To handle 12h under 30 DEG C of illumination, second of condition is that 12h is handled under 20 DEG C of dark, described to sprout pearl of the matrix by 1 parts by weight Rock and the water of 2.5-3 parts by weight are well mixed obtained;
    1 time and 0.05-0.5 parts by weight water is added every 7 days depressurizations are ventilative in the Stratificated treatment;
    Seed disinfection is carried out before the Stratificated treatment, tsaoko seed is put into 0.1% liquor potassic permanganate and soaks 25- 30min, then rinsed well with clear water, ventilation disposed within, dry tsaoko the surface of the seed moisture;
    Seed treatment is carried out before the seed disinfection, the tsaoko fruit of fresh mature no disease and pests harm is gathered, by the tsaoko Fruit is placed in room temperature in ventilative Polywoven Bag and stacked 2-3 days, divests outside rind, obtains seed group, the seed group is put 12-18 hours are stacked in the container of cleaning, seed group is then put into clear water and washed by rubbing with the hands, remove pulp and aril, are rejected miscellaneous Matter and flat grain, obtain complete full tsaoko seed;
    Watered 3 times into polybag daily in tsaoko fruit stacking process.
  2. 2. the method for promoting tsaoko seed to sprout as claimed in claim 1, it is characterised in that the perlite and water pass through 121 DEG C, the processing of 20min high-temperature sterilizations.
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CN105875166B (en) * 2016-05-16 2019-02-19 云南省林业科学院 A kind of bionical method for culturing seedlings of tsaoko
CN107926500B (en) * 2017-11-09 2019-10-08 红河学院 A kind of high-efficient method for cultivating of tsaoko seedling
CN108738460A (en) * 2018-04-23 2018-11-06 安徽咱家田生态农业有限公司 A method of promote Snakegourd Fruit seed to sprout
CN114258753A (en) * 2021-12-17 2022-04-01 广西壮族自治区中国科学院广西植物研究所 Method for promoting germination of elaeagnus pungens seeds
CN115119682A (en) * 2022-07-21 2022-09-30 江苏省农业科学院 Method for promoting pearl pear seed germination through hormone and temperature-changing treatment

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CN103262735A (en) * 2013-05-16 2013-08-28 黄振忠 Amomum tsao-ko seedling cultivating method
CN104025761B (en) * 2014-06-04 2015-10-21 广西壮族自治区药用植物园 A kind of method promoting pokeroot seed germination
CN104186061B (en) * 2014-08-11 2016-05-04 广西壮族自治区药用植物园 A kind of method that improves meadowrueleaf corydalis root seed germination

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Application publication date: 20150819

Assignee: Guangxi Jiansen Forestry Development Co.,Ltd.

Assignor: GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS

Contract record no.: X2023980045534

Denomination of invention: A Method for Promoting the Germination of Grass Fruit Seeds

Granted publication date: 20171117

License type: Common License

Record date: 20231106