CN104838762A - Method for promoting germination of Amomum tsaoko Crevost et Lemarie seed - Google Patents

Method for promoting germination of Amomum tsaoko Crevost et Lemarie seed Download PDF

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CN104838762A
CN104838762A CN201510261360.1A CN201510261360A CN104838762A CN 104838762 A CN104838762 A CN 104838762A CN 201510261360 A CN201510261360 A CN 201510261360A CN 104838762 A CN104838762 A CN 104838762A
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seed
tsaoko
perlite
germination
water
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CN104838762B (en
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潘春柳
邓志军
黄燕芬
韦坤华
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Guangxi Botanical Garden of Medicinal Plants
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Guangxi Botanical Garden of Medicinal Plants
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Abstract

The invention relates to a method for promoting germination of Amomum tsaoko Crevost et Lemarie seeds. The method comprises the steps of fully mixing the Amomum tsaoko Crevost et Lemarie seeds with wet perlite according to a volume ratio of 1 to 3 during stratification treatment, sealing the mixture and placing the mixture at 10 to 15 DEG C for stratification for 90 to 100 days, wherein the wet perlite is obtained by uniformly mixing 1 part of perlite by weight with 1.4 to 2 parts of water by weight; sowing the Amomum tsaoko Crevost et Lemarie seeds after stratification treatment into germination medium and performing alternate culture for 28 days under two conditions, including light treatment for 12h under a first condition of 30 DEG C and dark treatment for 12h under a second condition of 20 DEG C, wherein the germination medium is obtained by uniformly mixing 1 part of perlite by weight with 2.5 to 3 parts of water by weight. The method for promoting germination of Amomum tsaoko Crevost et Lemarie seeds provided by the invention has the advantages that the germination rate is high, the operation is simple, the cost is low, the manual operation is facilitated, and the application value to the seedling raising and large-scale cultivation of the Amomum tsaoko Crevost et Lemarie seeds is very great.

Description

A kind of method promoting tsaoko seed germination
Technical field
The invention belongs to field of plant growing technology, be specifically related to a kind of method promoting tsaoko seed germination.
Background technology
Tsaoko (Amomum tsaoko Crevost et Lemarie) is under the jurisdiction of Zingiber (Zingiberaceae) Amomum (Amomum), originates in the provinces and regions such as Yunnan, Guangxi, Guizhou.Be used as medicine with dry mature fruit, have in eliminating dampness temperature, the effect of preventing malaria eliminating phlegm, for internal retention of cold wetness, abdominal distention, ruffian is completely vomitted, malaria fever and chills and pestilence heating.In addition, tsaoko also can do flavoring, and complete stool can extract aromatic oil.In production, tsaoko, but can not dry storage after tsaoko seed collection based on seminal propagation, dries easily to lose vigor afterwards, and the tsaoko seed of freshly harvested is in resting state, and directly sowing is germinateed slow, and germination rate is low, emerges irregular.Now there are some researches show, GA 3, H 2sO 4, NaOH or wet husky the sprouting that process all can promote tsaoko seed such as to hide, but poor effect, the highest germination rate only about 50%.
Summary of the invention
The object of the invention is for the deficiencies in the prior art, a kind of method promoting tsaoko seed germination is provided.
A kind of method promoting tsaoko seed germination provided by the present invention, comprises the following steps:
Be fully to mix at 1: 3 by volume with wet perlite when carrying out Stratificated treatment by tsaoko seed, lamination 90-100 days at sealing is placed on 10-15 DEG C, described wet perlite is mixed by the perlite of 1 weight portion and the water of 1.4-2 weight portion and obtains.
Preferably, the method of described promotion tsaoko seed germination, tsaoko seed after Stratificated treatment is sown into and sprouts in matrix, alternate culture 28 days under two conditions, the first condition is process 12h under 30 DEG C of illumination, the second condition is process 12h under 20 DEG C of dark, and described sprouting matrix mixes obtained by the perlite of 1 weight portion and the water of 2.5-3 weight portion.
Preferably, the method for described promotion tsaoko seed germination, described perlite and water are through 121 DEG C, the process of 20min high-temperature sterilization.
Preferably, the method for described promotion tsaoko seed germination, carried out seed disinfection before described Stratificated treatment, the liquor potassic permanganate that tsaoko seed puts into 0.1% is soaked 25-30min, then rinse well with clear water, be placed in room ventilation place, dry tsaoko the surface of the seed moisture.
Preferably, the method of described promotion tsaoko seed germination, carried out seed treatment before described seed disinfection, gathered the tsaoko fruit of fresh mature without damage by disease and insect, described tsaoko fruit is placed in ventilative Polywoven Bag room temperature and stacks 2-3 days, divest outside rind, obtain seed group, described seed group is placed in clean container and stacks 12-18 hour, then seed group is put into clear water to wash by rubbing with the hands, remove pulp and arillus, reject impurity and flat grain, obtain complete full tsaoko seed.
Preferably, the method for described promotion tsaoko seed germination, in tsaoko fruit stacking process, every day waters 3 times in plastic sack.
Preferably, the method for described promotion tsaoko seed germination, breathes freely 1 time every 7 days depressurizations and adds 0.05-0.5 weight parts water in described Stratificated treatment.
The present invention at least comprises following beneficial effect:
The first, tsaoko seed is mixed in wet perlite carries out Stratificated treatment, because of perlitic water conservation, good permeability, can prevent seed from rotting in lamination process, the dormancy of seed be removed, effectively ensure that the vigor of tsaoko seed, promote the sprouting of seed.
The second, adopt the light dark that relents to replace vernalization, effectively can improve the vigor of tsaoko seed endoenzyme, promote that nutriment transforms, improve germination rate.
Three, after divesting tsaoko fruit outside rind, seed group is placed in clean container and stacks 12-18 hour, due to the effect of enzyme, bacterium and temperature etc. in stacking process, seed group's pulp softening, seed group is put into clear water after stacking to wash by rubbing with the hands and can remove outer arillus and pulp, wash by rubbing with the hands with existing employing sand and ash, the seed impurity that this method obtains is few again, totally, later stage seed selection is convenient to.
Four, carried out disinfection by tsaoko seed, perlite and water carry out high-temperature sterilization process, effectively can reduce seed going mouldy in lamination or pregermination procedure.
Five, the method for promotion tsaoko seed germination provided by the invention, germination rate is more than 80%, and simple to operate, and cost is low, is easy to manual operation, is of very high actual application value to tsaoko seed seedling-raising, large-scale planting.
Part is embodied by explanation below by other advantages of the present invention, target and feature, part also will by research and practice of the present invention by those skilled in the art is understood.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail, can implement according to this with reference to specification word to make those skilled in the art.
Experimental technique described in following embodiment, if no special instructions, is conventional method, described reagent and material, if no special instructions, all can obtain from commercial channels.
Embodiment 1:
Promote a method for tsaoko seed germination, comprise the following steps:
Be fully to mix at 1: 3 by volume with wet perlite when carrying out Stratificated treatment by tsaoko seed, lamination 100 days at sealing is placed on 10 DEG C, described wet perlite is mixed by the perlite of 1 weight portion and the water of 1.4 weight portions and obtains.Tsaoko seed is mixed in wet perlite and carries out Stratificated treatment, because of perlite water conservation, good permeability, can prevent seed from rotting in lamination process, the dormancy of seed be removed, effectively ensure that the vigor of tsaoko seed, promote the sprouting of seed.
The method of described promotion tsaoko seed germination, tsaoko seed after Stratificated treatment is sown into and sprouts in matrix, alternate culture 28 days under two conditions, the first condition is process 12h under 30 DEG C of illumination, the second condition is process 12h under 20 DEG C of dark, and described sprouting matrix mixes obtained by the perlite of 1 weight portion and the water of 2.5 weight portions.The employing light dark that relents replaces vernalization, effectively can improve the vigor of tsaoko seed endoenzyme, promotes that nutriment transforms, improves germination rate.
The method of described promotion tsaoko seed germination, described perlite and water are through 121 DEG C, the process of 20min high-temperature sterilization.
The method of described promotion tsaoko seed germination, before described Stratificated treatment, carry out seed disinfection, the liquor potassic permanganate that tsaoko seed puts into 0.1% is soaked 25min, then rinses well with clear water, be placed in room ventilation place, dry tsaoko the surface of the seed moisture.Tsaoko seed is carried out disinfection, effectively can reduce seed going mouldy in lamination or pregermination procedure.
The method of described promotion tsaoko seed germination, seed treatment was carried out before described seed disinfection, gather the tsaoko fruit of fresh mature without damage by disease and insect, described tsaoko fruit is placed in ventilative Polywoven Bag room temperature and stacks 2 days, every day waters 3 times in plastic sack, divest outside rind, obtain seed group, described seed group is placed in clean container to stack 12 hours, then seed group is put into clear water to wash by rubbing with the hands, remove pulp and arillus, reject impurity and flat grain, obtain complete full tsaoko seed.After divesting tsaoko fruit outside rind, seed group is placed in clean container to stack, due to the effect of enzyme, bacterium and temperature etc., seed group's pulp softening, after stacking, seed group is put into clear water to wash by rubbing with the hands and can remove outer arillus and pulp, wash by rubbing with the hands with existing employing sand and ash, the seed impurity that this method obtains is few, totally, later stage seed selection is convenient to.
The method of described promotion tsaoko seed germination, breathes freely 1 time every 7 days depressurizations and adds 0.05 weight parts water in described Stratificated treatment.
Embodiment 2:
Be fully to mix at 1: 3 by volume with wet perlite when carrying out Stratificated treatment by tsaoko seed, lamination 95 days at sealing is placed on 12.5 DEG C, described wet perlite is mixed by the perlite of 1 weight portion and the water of 1.7 weight portions and obtains.Tsaoko seed is mixed in wet perlite and carries out Stratificated treatment, because of perlite water conservation, good permeability, can prevent seed from rotting in lamination process, the dormancy of seed be removed, effectively ensure that the vigor of tsaoko seed, promote the sprouting of seed.
The method of described promotion tsaoko seed germination, tsaoko seed after Stratificated treatment is sown into and sprouts in matrix, alternate culture 28 days under two conditions, the first condition is process 12h under 30 DEG C of illumination, the second condition is process 12h under 20 DEG C of dark, and described sprouting matrix mixes obtained by the perlite of 1 weight portion and the water of 2.8 weight portions.The employing light dark that relents replaces vernalization, effectively can improve the vigor of tsaoko seed endoenzyme, promotes that nutriment transforms, improves germination rate.
The method of described promotion tsaoko seed germination, described perlite and water are through 121 DEG C, the process of 20min high-temperature sterilization.
The method of described promotion tsaoko seed germination, before described Stratificated treatment, carry out seed disinfection, the liquor potassic permanganate that tsaoko seed puts into 0.1% is soaked 28min, then rinses well with clear water, be placed in room ventilation place, dry tsaoko the surface of the seed moisture.Tsaoko seed is carried out disinfection, effectively can reduce seed going mouldy in lamination or pregermination procedure.
The method of described promotion tsaoko seed germination, seed treatment was carried out before described seed disinfection, gather the tsaoko fruit of fresh mature without damage by disease and insect, described tsaoko fruit is placed in ventilative Polywoven Bag room temperature and stacks 2 days, every day waters 3 times in plastic sack, divest outside rind, obtain seed group, described seed group is placed in clean container to stack 15 hours, then seed group is put into clear water to wash by rubbing with the hands, remove pulp and arillus, reject impurity and flat grain, obtain complete full tsaoko seed.After divesting tsaoko fruit outside rind, seed group is placed in clean container to stack, due to the effect of enzyme, bacterium and temperature etc., seed group's pulp softening, after stacking, seed group is put into clear water to wash by rubbing with the hands and can remove outer arillus and pulp, wash by rubbing with the hands with existing employing sand and ash, the seed impurity that this method obtains is few, totally, later stage seed selection is convenient to.
The method of described promotion tsaoko seed germination, breathes freely 1 time every 7 days depressurizations and adds 0.1 weight parts water in described Stratificated treatment.
Embodiment 3:
Be fully to mix at 1: 3 by volume with wet perlite when carrying out Stratificated treatment by tsaoko seed, lamination 90 days at sealing is placed on 15 DEG C, described wet perlite is mixed by the perlite of 1 weight portion and the water of 2 weight portions and obtains.Tsaoko seed is mixed in wet perlite and carries out Stratificated treatment, because of perlite water conservation, good permeability, can prevent seed from rotting in lamination process, the dormancy of seed be removed, effectively ensure that the vigor of tsaoko seed, promote the sprouting of seed.
The method of described promotion tsaoko seed germination, tsaoko seed after Stratificated treatment is sown into and sprouts in matrix, alternate culture 28 days under two conditions, the first condition is process 12h under 30 DEG C of illumination, the second condition is process 12h under 20 DEG C of dark, and described sprouting matrix mixes obtained by the perlite of 1 weight portion and the water of 3 weight portions.The employing light dark that relents replaces vernalization, effectively can improve the vigor of tsaoko seed endoenzyme, promotes that nutriment transforms, improves germination rate.
The method of described promotion tsaoko seed germination, described perlite and water are through 121 DEG C, the process of 20min high-temperature sterilization.
The method of described promotion tsaoko seed germination, before described Stratificated treatment, carry out seed disinfection, the liquor potassic permanganate that tsaoko seed puts into 0.1% is soaked 30min, then rinses well with clear water, be placed in room ventilation place, dry tsaoko the surface of the seed moisture.Tsaoko seed is carried out disinfection, effectively can reduce seed going mouldy in lamination or pregermination procedure.
The method of described promotion tsaoko seed germination, seed treatment was carried out before described seed disinfection, gather the tsaoko fruit of fresh mature without damage by disease and insect, described tsaoko fruit is placed in ventilative Polywoven Bag room temperature and stacks 3 days, every day waters 3 times in plastic sack, divest outside rind, obtain seed group, described seed group is placed in clean container to stack 18 hours, then seed group is put into clear water to wash by rubbing with the hands, remove pulp and arillus, reject impurity and flat grain, obtain complete full tsaoko seed.After divesting tsaoko fruit outside rind, seed group is placed in clean container to stack, due to the effect of enzyme, bacterium and temperature etc., seed group's pulp softening, after stacking, seed group is put into clear water to wash by rubbing with the hands and can remove outer arillus and pulp, wash by rubbing with the hands with existing employing sand and ash, the seed impurity that this method obtains is few, totally, later stage seed selection is convenient to.
The method of described promotion tsaoko seed germination, breathes freely 1 time every 7 days depressurizations and adds 0.2 weight parts water in described Stratificated treatment.
Embodiment 4:
Be fully to mix at 1: 3 by volume with wet perlite when carrying out Stratificated treatment by tsaoko seed, lamination 100 days at sealing is placed on 15 DEG C, described wet perlite is mixed by the perlite of 1 weight portion and the water of 2 weight portions and obtains.Tsaoko seed is mixed in wet perlite and carries out Stratificated treatment, because of perlite water conservation, good permeability, can prevent seed from rotting in lamination process, the dormancy of seed be removed, effectively ensure that the vigor of tsaoko seed, promote the sprouting of seed.
The method of described promotion tsaoko seed germination, tsaoko seed after Stratificated treatment is sown into and sprouts in matrix, alternate culture 28 days under two conditions, the first condition is process 12h under 30 DEG C of illumination, the second condition is process 12h under 20 DEG C of dark, and described sprouting matrix mixes obtained by the perlite of 1 weight portion and the water of 3 weight portions.The employing light dark that relents replaces vernalization, effectively can improve the vigor of tsaoko seed endoenzyme, promotes that nutriment transforms, improves germination rate.
The method of described promotion tsaoko seed germination, described perlite and water are through 121 DEG C, the process of 20min high-temperature sterilization.
The method of described promotion tsaoko seed germination, before described Stratificated treatment, carry out seed disinfection, the liquor potassic permanganate that tsaoko seed puts into 0.1% is soaked 30min, then rinses well with clear water, be placed in room ventilation place, dry tsaoko the surface of the seed moisture.Tsaoko seed is carried out disinfection, effectively can reduce seed going mouldy in lamination or pregermination procedure.
The method of described promotion tsaoko seed germination, seed treatment was carried out before described seed disinfection, gather the tsaoko fruit of fresh mature without damage by disease and insect, described tsaoko fruit is placed in ventilative Polywoven Bag room temperature and stacks 3 days, every day waters 3 times in plastic sack, divest outside rind, obtain seed group, described seed group is placed in clean container to stack 18 hours, then seed group is put into clear water to wash by rubbing with the hands, remove pulp and arillus, reject impurity and flat grain, obtain complete full tsaoko seed.After divesting tsaoko fruit outside rind, seed group is placed in clean container to stack, due to the effect of enzyme, bacterium and temperature etc., seed group's pulp softening, after stacking, seed group is put into clear water to wash by rubbing with the hands and can remove outer arillus and pulp, wash by rubbing with the hands with existing employing sand and ash, the seed impurity that this method obtains is few, totally, later stage seed selection is convenient to.
The method of described promotion tsaoko seed germination, breathes freely 1 time every 7 days depressurizations and adds 0.5 weight parts water in described Stratificated treatment.
Embodiment 5:
Be fully to mix at 1: 3 by volume with wet perlite when carrying out Stratificated treatment by tsaoko seed, lamination 100 days at sealing is placed on 15 DEG C, described wet perlite is mixed by the perlite of 1 weight portion and the water of 2 weight portions and obtains.Tsaoko seed is mixed in wet perlite and carries out Stratificated treatment, because of perlite water conservation, good permeability, can prevent seed from rotting in lamination process, the dormancy of seed be removed, effectively ensure that the vigor of tsaoko seed, promote the sprouting of seed.
The method of described promotion tsaoko seed germination, tsaoko seed after Stratificated treatment is sown into and sprouts in matrix, alternate culture 28 days under two conditions, the first condition is process 12h under 30 DEG C of illumination, the second condition is process 12h under 20 DEG C of dark, and described sprouting matrix mixes obtained by the perlite of 1 weight portion and the water of 3 weight portions.The employing light dark that relents replaces vernalization, effectively can improve the vigor of tsaoko seed endoenzyme, promotes that nutriment transforms, improves germination rate.
The method of described promotion tsaoko seed germination, seed treatment was carried out before described Stratificated treatment, gather the tsaoko fruit of fresh mature without damage by disease and insect, described tsaoko fruit is placed in ventilative Polywoven Bag room temperature and stacks 3 days, every day waters 3 times in plastic sack, divest outside rind, obtain seed group, described seed group is placed in clean container to stack 18 hours, then seed group is put into clear water to wash by rubbing with the hands, remove pulp and arillus, reject impurity and flat grain, obtain complete full tsaoko seed.After divesting tsaoko fruit outside rind, seed group is placed in clean container to stack, due to the effect of enzyme, bacterium and temperature etc., seed group's pulp softening, after stacking, seed group is put into clear water to wash by rubbing with the hands and can remove outer arillus and pulp, wash by rubbing with the hands with existing employing sand and ash, the seed impurity that this method obtains is few, totally, later stage seed selection is convenient to.
The method of described promotion tsaoko seed germination, breathes freely 1 time every 7 days depressurizations and adds 0.5 weight parts water in described Stratificated treatment.
Comparative example 1:
Beneficial effect of the present invention is further illustrated by comparative example.Wherein, common sprouting method is directly sown in wet sand by fresh tsaoko seed to sprout; It is be sown in perlite by fresh tsaoko seed to sprout that perlite sprouts method; Wet sand bed area method is on the basis of embodiment 5, Stratificated treatment is used instead common wet sand bed and amasss.
The different germination method of table 1 is on the impact of tsaoko seed germination rate
As shown in Table 1, the method for tsaoko seed germination of the present invention, compared with common sprouting method, germination rate improves 78-84%, improves 47-53% compared with sprouting method with perlite, and germination rate improves 39.5-45.5% compared with wet sand bed area method.
Although embodiment of the present invention are open as above, it is not restricted to listed in specification and embodiment utilization.It can be applied to various applicable the field of the invention completely.For those skilled in the art, can easily realize other amendment.Therefore do not deviating under the universal that claim and equivalency range limit, the present invention is not limited to specific details and illustrates here and the example described.

Claims (7)

1. one kind promotes the method for tsaoko seed germination, it is characterized in that, be fully to mix at 1: 3 by volume with wet perlite when carrying out Stratificated treatment by tsaoko seed, lamination 90-100 days at sealing is placed on 10-15 DEG C, described wet perlite is mixed by the perlite of 1 weight portion and the water of 1.4-2 weight portion and obtains.
2. the method promoting tsaoko seed germination as claimed in claim 1, it is characterized in that, tsaoko seed after Stratificated treatment is sown into and sprouts in matrix, alternate culture 28 days under two conditions, the first condition is process 12h under 30 DEG C of illumination, the second condition is process 12h under 20 DEG C of dark, and described sprouting matrix mixes obtained by the perlite of 1 weight portion and the water of 2.5-3 weight portion.
3. the method promoting tsaoko seed germination as claimed in claim 2, it is characterized in that, described perlite and water are through 121 DEG C, the process of 20min high-temperature sterilization.
4. the method promoting tsaoko seed germination as claimed in claim 3, it is characterized in that, seed disinfection was carried out before described Stratificated treatment, the liquor potassic permanganate that tsaoko seed puts into 0.1% is soaked 25-30min, then rinse well with clear water, be placed in room ventilation place, dry tsaoko the surface of the seed moisture.
5. the method promoting tsaoko seed germination as claimed in claim 4, it is characterized in that, seed treatment was carried out before described seed disinfection, gather the tsaoko fruit of fresh mature without damage by disease and insect, described tsaoko fruit is placed in ventilative Polywoven Bag room temperature and stacks 2-3 days, divest outside rind, obtain seed group, described seed group is placed in clean container and stacks 12-18 hour, then seed group is put into clear water to wash by rubbing with the hands, remove pulp and arillus, reject impurity and flat grain, obtain complete full tsaoko seed.
6. the method promoting tsaoko seed germination as claimed in claim 5, it is characterized in that, in tsaoko fruit stacking process, every day waters 3 times in plastic sack.
7. the method promoting tsaoko seed germination as claimed in claim 6, is characterized in that, breathes freely 1 time and adds 0.05-0.5 weight parts water in described Stratificated treatment every 7 days depressurizations.
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CN105875166A (en) * 2016-05-16 2016-08-24 云南省林业科学院 Amomum tsao-ko biomimetic seedling culturing method
CN107926500A (en) * 2017-11-09 2018-04-20 红河学院 A kind of high-efficient method for cultivating of tsaoko seedling
CN108738460A (en) * 2018-04-23 2018-11-06 安徽咱家田生态农业有限公司 A method of promote Snakegourd Fruit seed to sprout
CN114258753A (en) * 2021-12-17 2022-04-01 广西壮族自治区中国科学院广西植物研究所 Method for promoting germination of elaeagnus pungens seeds
CN115119682A (en) * 2022-07-21 2022-09-30 江苏省农业科学院 Method for promoting pearl pear seed germination through hormone and temperature-changing treatment

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105875166A (en) * 2016-05-16 2016-08-24 云南省林业科学院 Amomum tsao-ko biomimetic seedling culturing method
CN105875166B (en) * 2016-05-16 2019-02-19 云南省林业科学院 A kind of bionical method for culturing seedlings of tsaoko
CN107926500A (en) * 2017-11-09 2018-04-20 红河学院 A kind of high-efficient method for cultivating of tsaoko seedling
CN107926500B (en) * 2017-11-09 2019-10-08 红河学院 A kind of high-efficient method for cultivating of tsaoko seedling
CN108738460A (en) * 2018-04-23 2018-11-06 安徽咱家田生态农业有限公司 A method of promote Snakegourd Fruit seed to sprout
CN114258753A (en) * 2021-12-17 2022-04-01 广西壮族自治区中国科学院广西植物研究所 Method for promoting germination of elaeagnus pungens seeds
CN115119682A (en) * 2022-07-21 2022-09-30 江苏省农业科学院 Method for promoting pearl pear seed germination through hormone and temperature-changing treatment

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Application publication date: 20150819

Assignee: Guangxi Jiansen Forestry Development Co.,Ltd.

Assignor: GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS

Contract record no.: X2023980045534

Denomination of invention: A Method for Promoting the Germination of Grass Fruit Seeds

Granted publication date: 20171117

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Record date: 20231106