CN102577698A - Treatment method of white flower loropetalum chinense seeds - Google Patents

Abstract

The invention provides a treatment method of white flower loropetalum chinense seeds, comprising the following steps of: soaking white flower loropetalum chinense seeds by acid solution and washing by water, carrying out accelerating germination in a lamination way by mixed matrix prepared from sands and pearlite, laminating for 4 months in a warming way, and laminating for 2 months at low temperature. According to the method, the permeability of the flower loropetalum chinense seeds is improved, the rest period of the seeds is broken, and the germination is promoted. The experiment proves that the germinating rate of the white flower loropetalum chinense seeds is improved.

Description

White rubrum seed treatment

Technical field:

The invention relates to an artificial white rubrum seed treatment.

Background technology:

White rubrum (Loropetalum? Chinens), also known as white rubrum, is a rubrum is Hamamelidaceae (Hamamelidaceae) rubrum evergreen shrub of the genus, distributed in Jiangxi Xiangya tropical regions.

White rubrum of flowers, stems, leaves, branches have important medicinal value, and now for this precious plant breeding technology research rarely reported in the literature.

White rubrum seeds in the natural wild state hard sprouting, slow growth, although adaptable but the primary biological production is very low, mainly due to white rubrum seeds have 3 to 4 years of seed dormancy, coupled with population ecology environment has become more severe, causing wild wild white rubrum can survive a lot of breeding.So white rubrum breeding techniques, especially seed germination process are in dire need of technical methods.

Summary of the invention:

The object of the present invention is to provide a white rubrum seed treatment method, the germination process, the seed white rubrum facilitate planting and seedling.

White rubrum seed germination process is as follows:

In white rubrum before sowing the seeds for germination process the following procedure:

1) etch treatment: white rubrum seeds with 35% sulfuric acid solution soak 3h;

2) flushing: the seed behind the acid etching is washed 24h with the water that flows;

3) lamination is handled: after the seed after acid etching and the flushing was dried surface moisture, the mixed-matrix that is made into sand and perlite carried out lamination vernalization, and sand and perlitic amount ratio are 1: 3 (volume ratio);

Preferred lamination processing method is:

A, before lamination, said matrix is used in advance the disinfecting solution of potassium permanganate of 30ppm;

B, use water-wet matrix;

Its preferred humidity is: with holding tight matrix anhydrous oozing to can become bulk the time;

C, matrix are mixed with the kind sublayering, i.e. one deck seed one deck sand, or mix matrix and seed thoroughly the back lamination;

D, lamination height are not less than 35cm;

4) variable temperature treatment: 25 ℃ ± 1 ℃ warm stratification 4 months and 5 ℃ ± 1 ℃ cold stratification two months, the white rubrum the highest germination rate (48.7% -52.7%).

Simultaneously, in the earthing of seeds process, keep matrix moistening.

The inventions on white rubrum seed stratification variable temperature prior to moderate acid treatment and other methods to improve the permeability of the seed, to break seed dormancy and promote germination, and improve seed germination.

The method of the present invention provides a process for white rubrum seeds, as well as through the method of storage of seeds in the nursery in white rubrum were received very good results:

1, bud ratio is higher

Can germinate fast, take root, germination rate also high (48.7%-52.7%);

2, easy to raise, survival rate height

The grow seedlings survival rate of wood of this method can reach (95 ± 3) more than the %, is significantly improved than survival rate (63 ± 3) % of conventional breeding.

3, growth of seedling rapid speed

Other kinds of growth of seedling period ratio will shorten greatly, and actual measurement year high growth 30-50cm reaches as high as 50-70cm, and growth vigor is obvious.

4, planting conditions is wide, has tolerance to cold

Seedling all can fine growth under-8 ℃～32 ℃ temperature conditions.

Embodiment

White by the following examples of the treatment of seed rubrum Examples further detail.But the present invention is not limited to this.

Example 1 white rubrum seed treatment

1.1 seed collection and selection

White rubrum mature seeds easily play Yi, naturally low recovery, to improve the white rubrum seed harvesting efficiency, ensure seed quality, in its cones approaching maturity, with a paper bag pack fruit or fruit bud before cracking the use of certain methods, seed collection.

1.2 adopt post processing

A, air-dry: the fruit bud clothing of the seed of gathering is removed, and combined processing to take out seed, be placed on the ventilation then and dry in the shade.

B, cleaning: the seed to drying in the shade is further selected again, and removal of contamination reaches more than 95% purity.

C, seed storage: seed air-dry and that cleared up is placed on low temperature, drying, can preserves in the warehouse of lucifuge; Duration of storage should diligently be checked, prevents that the mold of seed is rotten.Storage period must not be above 1 year.

1.3 seed is selected

First-class seed.Thousand kernel weight is not less than 230 grams, and plumpness is not less than 96%, and vigor is not less than 99%, and cleanliness is not less than 95%, and water content is between 35%-14%.The color and luster of seed is bright pitchy, free from extraneous odour, no infected seed.

2 presproutings of seeds are handled

Prior to seeding, the presprouting of seeds that must make following program is handled:

A, acid etching: the pure seed that will gather then or preserve soaks 3h with 35% sulfuric acid solution, then through flowing water flushing 24h.

B, lamination are handled: the seed after acid etching and the flushing is dried surface moisture, use the mixed-matrix of husky and perlite (1: 3) to carry out lamination vernalization.Before the lamination; Matrix is used the disinfecting solution of potassium permanganate of 30ppm in advance, and during lamination, matrix is wetting to holding tightly agglomerating but till not having water droplet with running water; But matrix is mixed with seed layering (one deck seed one deck sand) or mix matrix and seed thoroughly the back storage, and the lamination height is not less than 35cm.

C, variable temperature treatment: 25 ℃ ± 1 ℃ warm stratification 4 months and 5 ℃ ± 1 ℃ cold stratification two months, the white rubrum the highest germination rate (48.7% -52.7%).Simultaneously, in the earthing of seeds process, keep matrix moistening.

Example 2 white rubrum seed storage

Reach the seed of vernalization index, can not sow then, do storage for the winter well.Duration of storage is earlier through physiology after-ripening (temperature 0-5 ℃), and is frozen then.

For avoiding to sow then the seed mutation of physiology after-ripening, should continue low temperature drying storage (5 ℃-9 ℃ of temperature).Can not directly be exposed under the normal temperature (10 ℃-25 ℃).

Example 3 white rubrum nursery

Sowing time

Sow in spring:, can grow seedlings after soil thaws at mid or late March.

Summer sowing: carry out in mid or late June.

Autumn sowing: before early October is extremely freeze-up, can grow seedlings.

Type of seeding

Program request: cultivate one year seedling, adopt 3cm * 5cm or 4cm * 4cm program request; Cultivate 2-3 and give birth to seedling employing 4cm * 5cm or 5cm * 5cm centimetre of program request.

Drilling: line-spacing 0.8 * 0.8m, broadcast into 200-300 grain seed for every square metre.Thickness of earth covering: 3-7 centimetre, every square metre with kind of a 75-100g.

Broadcast sowing: loosen the soil to 4cm dark about, seed evenly is sprinkled into, on cover fine earth and fill and lead up, every square metre with kind of a 150-250g.Every mu 800～1050 strain.

The attention problem

The seedbed will meet standard, select for use enrich full, the seed that embryonic development is sound, the seed that radicle has stretched out kind of skin should not use.After planting, it is consistent that thickness of earth-fill cover is wanted, and thickness will suit.During spring sowing, soil drought will be poured water in advance, and for the smooth sprouting of seed provides sufficient moisture, volt seedbed face will cover, and wants winter protection after the autumn sowing.

Seedling management

Dig earth 1-2 time of A, the dark root of soil disinfection utilizes the nature solar heat to be exposed to the sun 4-5 day, effectively kills various pathogeny bacterium and insect.

B, the weeding of keeping a full stand of seedings, seed germination are after 20 days, and about every 7-10 days once.

C, the suitable control water that shades satisfy the demand of seedling to moisture, to the needs of plant nutrition.

D, reasonably use the agricultural chemicals of low-residual, prevent sprout term disease, insect pest.

This method of bringing out the white rubrum seedlings easy to grow, high survival rate.

What under the operation sequence of regulation, can germinate relatively fast takes root, and compares the survival rate (63 ± 3%) of conventional breeding, and the survival rate of this method can reach more than 95 ± 3%.And the growth of seedling rapid speed, growth cycle will shorten than other kinds greatly, and actual measurement year high growth 30-50cm reaches as high as 50-70cm, and growth vigor is obvious.Seedling planting conditions after improvement is wide simultaneously, can heat-resistingly resist cold (the seedling early growth environment is-8 ℃～32 ℃).

Claims (4)

1 A white rubrum seed processing method, program as follows:

1) etch treatment: white rubrum seeds with 35% sulfuric acid solution soak 3h;

2) flushing: the seed behind the acid etching is washed 24h with the water that flows;

3) after seed lamination processing: with step 2) dried surface moisture, the mixed-matrix that is made into sand and perlite carried out lamination vernalization, and sand and perlitic amount ratio are 1: 3 (volume ratio);

4) alternating temperature is handled: warm layer is long-pending 4 months under 25 ℃ ± 1 ℃ environment, then low temperature lamination 2 months under 5 ℃ ± 1 ℃ environment

2. the described processing method of claim 1, in the said lamination processing method:

1) before lamination, said matrix is used in advance the disinfecting solution of potassium permanganate of 30ppm;

2) use water-wet matrix, and in the earthing of seeds process, keep matrix moistening;

3) matrix is mixed with the kind sublayering, and mixed method is one deck seed one deck sand, or mixes matrix and seed thoroughly the back lamination;

3. the described processing method of claim 2, the humidity that said use water-wet matrix reaches is: with holding tight matrix anhydrous oozing to can become bulk the time.

4. the described processing method of claim 1, said lamination height is not less than 35cm.