CN104642267A - Industrial cultivation method for aphidius gifuensis ashmead - Google Patents
Industrial cultivation method for aphidius gifuensis ashmead Download PDFInfo
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- CN104642267A CN104642267A CN201510065048.5A CN201510065048A CN104642267A CN 104642267 A CN104642267 A CN 104642267A CN 201510065048 A CN201510065048 A CN 201510065048A CN 104642267 A CN104642267 A CN 104642267A
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- 241001305034 Aphidius gifuensis Species 0.000 title claims abstract description 171
- 238000012364 cultivation method Methods 0.000 title abstract 2
- 241001124076 Aphididae Species 0.000 claims abstract description 467
- 238000009395 breeding Methods 0.000 claims abstract description 62
- 238000000034 method Methods 0.000 claims abstract description 38
- 230000001488 breeding effect Effects 0.000 claims abstract description 34
- 238000012360 testing method Methods 0.000 claims abstract description 31
- 230000001954 sterilising effect Effects 0.000 claims abstract description 12
- 241000721621 Myzus persicae Species 0.000 claims abstract description 9
- 238000003860 storage Methods 0.000 claims abstract description 8
- 241000196324 Embryophyta Species 0.000 claims description 134
- 235000019504 cigarettes Nutrition 0.000 claims description 108
- 241000256844 Apis mellifera Species 0.000 claims description 53
- 230000003071 parasitic effect Effects 0.000 claims description 37
- 238000012856 packing Methods 0.000 claims description 29
- 241000208125 Nicotiana Species 0.000 claims description 28
- 238000002955 isolation Methods 0.000 claims description 26
- 230000024241 parasitism Effects 0.000 claims description 20
- 238000005286 illumination Methods 0.000 claims description 13
- 238000004659 sterilization and disinfection Methods 0.000 claims description 13
- 239000002054 inoculum Substances 0.000 claims description 11
- 230000008569 process Effects 0.000 claims description 11
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 10
- 201000010099 disease Diseases 0.000 claims description 9
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 9
- 238000010899 nucleation Methods 0.000 claims description 8
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- 238000004806 packaging method and process Methods 0.000 abstract 3
- 238000007605 air drying Methods 0.000 abstract 1
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- 241000220259 Raphanus Species 0.000 description 16
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 description 16
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- 238000011835 investigation Methods 0.000 description 15
- 238000005070 sampling Methods 0.000 description 15
- 241001600407 Aphis <genus> Species 0.000 description 12
- 235000013311 vegetables Nutrition 0.000 description 11
- 235000013601 eggs Nutrition 0.000 description 10
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- 235000019004 Ipomoea aquatica Nutrition 0.000 description 9
- 238000004519 manufacturing process Methods 0.000 description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- 239000003337 fertilizer Substances 0.000 description 7
- 230000027272 reproductive process Effects 0.000 description 7
- 241000238631 Hexapoda Species 0.000 description 5
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- FFSJPOPLSWBGQY-UHFFFAOYSA-N triazol-4-one Chemical compound O=C1C=NN=N1 FFSJPOPLSWBGQY-UHFFFAOYSA-N 0.000 description 4
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- 238000005516 engineering process Methods 0.000 description 2
- 244000037666 field crops Species 0.000 description 2
- 235000021393 food security Nutrition 0.000 description 2
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 2
- 230000010354 integration Effects 0.000 description 2
- 231100000225 lethality Toxicity 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- 241001425390 Aphis fabae Species 0.000 description 1
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- 102000002322 Egg Proteins Human genes 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
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- Biodiversity & Conservation Biology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses an industrial cultivation method for aphidius gifuensis ashmead. The method comprises the following steps: cultivating host plants for myzus persicae sulzer, and breeding myzus persicae sulzer on the host plants; breeding mummified aphids of aphidius gifuensis ashmead on the myzus persicae sulzer by taking the bred myzus persicae sulzer as hosts for breeding aphidius gifuensis ashmead; separating the bred mummified aphids from leaves of the host plants, and collecting the mummified aphids; sterilizing the collected mummified aphids, performing air-drying treatment, and performing irradiation treatment with ultraviolet light; rejecting the mummified aphids which are diseased, damaged and smaller, and transferring the mummified aphids into sub-packaging test tubes or uniformly spreading the mummified aphids on paper cards to form mummified aphid cards; storing the sub-packaging test tubes containing the mummified aphids or the mummified aphid cards, and performing packaging according to requirements. According to the method, the cultivation cycle of aphidius gifuensis ashmead is shortened, the collection, the storage and the transportation of aphidius gifuensis ashmead are integrated, the utilization rate of aphidius gifuensis ashmead is increased, and aphidius gifuensis ashmead tends to be developed towards commercial and industrial production.
Description
Technical field
The present invention relates to the technical field of biological control of agriculture and forestry insect, in particular to a kind of industrialization breeding method of aphidius gifuensis.
Background technology
Aphid is a kind of worldwide insect, agriculture and forestry plant mostly has aphid damage, aphid can cause plant nutrition to worsen, mainly on blade back and tender stem, suck crop juice leaf with adult and nymph, cause plant growth slowly or stagnate even deformity, affect the normal growth of plant, Plant death will be caused time serious, aphid also makes virus propagate carrier simultaneously, can propagate multiple virus disease and other disease, cause the popular of plant disease.For a long time mainly chemical pesticide is relied on to the control of aphid, chemical pesticide causes bio-diversity while elimination aphid to be reduced, and destroy ecotope, cause pollution of agricultural products, have a strong impact on food security, a large amount of pesticide resistance using chemical pesticide to enhance aphid simultaneously, and then make greatly to increase the difficulty of prevention and cure of aphid, cause vicious circle.
Along with the Harmless of agricultural production, best quality and Modern Trend become clear day by day, biological control and the comprehensive regulation will be realize significantly reducing the important channel that chemical pesticide uses.Aphidius gifuensis is the advantage parasite of cigarette aphid, take food juice and kill by ovum being originated in aphid body or control aphid, it all has control efficiency to little English grain aphid, greenbug, bean aphid, Chinese rose Macrosiphus spp etc., and it has very large potentiality in anti-eliminating aphis.At present, though breed aphidius gifuensis tentatively ripe in prior art, there is cycle length, low, the stiff aphid of stiff aphid density collects the problems such as difficulty, therefore, be badly in need of a kind of method aphidius gifuensis can being carried out merchandized handling at present.
Summary of the invention
There is cycle length in aphidius gifuensis reproductive process for solving in prior art, poor, the stiff aphid of stiff aphid density low, stiff aphid formation regularity collects difficulty, stores the problems such as transport difficult, Field information difficulty, not easily commercialization suitability for industrialized production, the object of the present invention is to provide a kind of industrialization breeding method of aphidius gifuensis.
For achieving the above object, provide a kind of industrialization breeding method of aphidius gifuensis in the embodiment of the present invention, the method comprises the following steps:
The host plant of S101, cultivation cigarette aphid, and on host plant, breed cigarette aphid;
S102, the host bred as aphidius gifuensis by the cigarette aphid bred, cigarette aphid breeds the stiff aphid of aphidius gifuensis;
S103, breed after stiff aphid be separated with host plant blade and collect stiff aphid;
S104, by after the stiff aphid sterilization after collecting, carry out air-dry process, finally use UV-irradiation process;
S105, the inspection of stiff aphid and packing: after rejecting the stiff aphid that damaged and individuality is less in spite of illness, move into stiff aphid in packing test tube or be evenly laid in and paper card makes stiff aphid block;
S106, will the packing test tube of stiff aphid be accommodated or stiff aphid card stores, and pack according to demand.
Breeding method provided by the invention is simple, what improve aphidius gifuensis breeds efficiency, overcome that to there is the cycle in aphidius gifuensis reproductive process in prior art long, stiff aphid density is low, it is poor that stiff aphid forms regularity, stiff aphid collects difficulty, store transport difficult, Field information difficulty, the not easily problem such as commercialization factorial praluction, shorten the cultivation cycle of aphidius gifuensis, in aphidius gifuensis production process, to the temperature of respectively breeding the stage, humidity, the weather conditions such as illumination and ventilation carry out standardization control, set up the biological property of aphidius gifuensis Cheng Feng and stiff aphid, realize stiff aphid to be separated with blade simultaneously, and then achieve the collection of aphidius gifuensis, the integration storing and transport, form aphidius gifuensis to produce and product quality inspection and control criterion, aphidius gifuensis is made to become a kind of commodity being convenient to circulate, achieve the commercialization of aphidius gifuensis, aphidius gifuensis applying on tobacco is made to achieve remarkable effect, be convenient to accumulate honeybee kind in a large number simultaneously, improve the availability of aphidius gifuensis, aphidius gifuensis the is possessed development trend of suitability for industrialized production.
Further, in step S101, the step of cultivating the host plant of cigarette aphid is:
1. leafy disease-resistant host plant seeding and seedling raising is selected;
2. transplant after seedling strain seedling to temperature be 20-30 DEG C, humidity is 30-70%, possess fly net isolation breed indoor, cultivate under the condition of natural lighting.
In the technical program, the cultivation of the host plant of cigarette aphid is further described and is limited, select leafy disease-resistant host plant, prevent in reproductive process, host plant occurs easily occurring illness, need in addition to spray triazolone liquid in host plant reproductive process, there is powdery mildew in control host plant, host plant can select tobacco, green vegetables, blue and white, radish or water spinach, when planting cultivation, tobacco, green vegetables, blue and white, the planting density of radish can be controlled in every square metre of 3-5 strain, the planting density of water spinach can be controlled in every square metre of 8-12 strain, and to the water and fertilizer management that host plant is suitable for, and rationally cultivate plants according to aphidius gifuensis merchandized handling in batches, improve cultivation efficiency.
Further, in step S101, the step of breeding cigarette aphid is:
1. aphid is connect: gather tobacco aphid kind and evenly inoculate with on the host plant cultivated, inoculum concentration is 5-10 head/plant;
2. cigarette aphid breeds: by connecing, the host plant after aphid moves into that temperature is 20-30 DEG C, humidity is 30-70%, to possess numerous aphid of fly net isolation indoor, freely breeds 10-15 days, namely obtain the cigarette aphid after breeding under the condition of natural lighting.
In the technical program, the breeding of cigarette aphid is further described, cigarette aphid is as the host of aphidius gifuensis, so must ensure that the cigarette aphid after breeding is that reproduction speed is fast, the aphid that vitality is strong, ensure that in later stage aphidius gifuensis production process, for aphidius gifuensis provides sufficient provenance, for ensureing the quality that cigarette aphid breeds, so need to breed environment for cigarette aphid provides good in reproductive process, shading screen should be added sun-proof during cigarette aphid breeds, gravity-flow ventilation, prevent aphidius gifuensis from entering and suppress cigarette aphid to breed, prevent cigarette aphid from fleeing, need to ensure growing up healthy and sound of host plant simultaneously, so should strict monitoring temperature in reproductive process, humidity, time etc.
Further, in step S102, the step that cigarette aphid breeds the stiff aphid of aphidius gifuensis is:
1. connecing honeybee: collection aphidius gifuensis worm kind is inoculated in numerous aphid indoor section and breeds on the host plant of cigarette aphid, connecing honeybee amount according to honeybee aphid than being 1:50-100;
2. aphidius gifuensis worm kind is bred: by connecing, the immigration of the host plant after honeybee temperature is 20-30 DEG C, humidity is 30-70%, it is indoor to possess numerous honeybee of fly net isolation, freely breeds 15 days, namely obtain the aphidius gifuensis worm kind after breeding under the condition of natural lighting;
3. the stiff aphid of aphidius gifuensis breeds: collect aphidius gifuensis worm kind breed after aphidius gifuensis, and being inoculated in numerous aphid indoor than 1:0.5-1.0 breeding on the host plant of cigarette aphid according to hero be female, connecing honeybee amount according to honeybee aphid than being 1:25-50;
4. expand numerous: by connecing, the host plant after honeybee moves into that temperature is 20-30 DEG C, humidity is 30-70%, to possess the expansion honeybee of fly net isolation indoor, 10 days are bred under the condition of natural lighting, when on host plant blade 80% aphid parasitic, and 50% start to form stiff aphid in by the aphid of parasitism, namely complete stiff aphid and breed.
In the technical program, careful description and restriction are done to the reproductive process of stiff aphid, first breeding of aphidius gifuensis be select build comparatively large, health status best as honeybee kind, then honeybee kind is bred in enormous quantities, after honeybee kind is bred, honeybee kind is seeded on cigarette aphid, breeds stiff aphid in enormous quantities, not only shorten the time that stiff aphid breeds, and improve the quality of stiff aphid.
Further, in step S103, stiff aphid with the step that host plant tobacco leaf was separated and collected stiff aphid is: be placed in stiff aphid separator by being with the host plant blade of stiff aphid, host plant blade is washed away with the supercharging shower nozzle that hydraulic pressure is 0.35MPa-0.50MPa, stiff aphid is separated with host plant blade, collects stiff aphid simultaneously.
In the technical program, be mainly used in describing stiff aphid to be separated with blade, stiff aphid is separated with blade to belong to and makes aphidius gifuensis enter one of key step of commercialization process, the workload be separated with blade due to stiff aphid is larger, so the present invention washes away blade by using water, thus blade being separated with stiff aphid, improve the efficiency of separation, also be convenient to collect stiff aphid simultaneously, practical.
Further, carry out stiff aphid Hui Fan while carrying out step S103, the steps include:
1. cut breeding with part by the aphid of parasitism and in aphid the host plant blade becoming stiff aphid with scissors, and be positioned in separator;
2. separator is placed on that temperature is 20-30 DEG C, humidity is 8-12 hour in 30-70% environment, aphid climbs in a large number on separator inwall, again separator is placed on that temperature is 28-30 DEG C, humidity is 2-4 hour in 30-70% environment, finally by the aphid in separator, parasitic aphid are inoculated in breed inoculation aphidius gifuensis host plant on continue to breed stiff aphid.
In the technical program, be mainly used in describing aphidius gifuensis host plant numerous stiff aphid last time, improve the availability of aphidius gifuensis and host aphid, improve the efficiency of breeding stiff aphid, improve the quantity that stiff aphid breeds simultaneously.
Further, in step S104, by after collecting stiff aphid carries out disinfection, step that is air-dry and UV-irradiation is:
1. sterilize: the stiff aphid after collection is placed in hydrogen peroxide and disinfects 1-2 minute;
2. air-dry: the stiff aphid uniform monolayers after sterilization to be covered in sub-sieve, and sub-sieve to be put in air dryer air-dry;
3. ultra violet lamp sub-sieve 3-5 minute is used.
In the technical program, the post-processed mainly for stiff aphid is further described, and the sterilization of stiff aphid, air-dry and Ultraviolet radiation are mainly used in preventing stiff aphid to be infected by bacteria, and promote the eclosion rate of aphidius gifuensis, ensure that product quality.
Further, in step S106, the band packing test tube of stiff aphid or stiff aphid blocks the step carrying out storing is: first the packing test tube of the stiff aphid of band or stiff aphid are stuck in that temperature is 5 DEG C, periodicity of illumination is after storing 15 days under the condition of L: D=9: 15, then temperature be 5-7 DEG C, humidity is 40-60%, periodicity of illumination be the condition of L: D=8-9: 15-16 under storage 25 days.
In the technical program, storage due to aphidius gifuensis is that aphidius gifuensis enters the difficult problem commercially produced, so the present invention is drawn by test of many times, packing test tube with stiff aphid or stiff aphid card first temperature be 5 DEG C, periodicity of illumination stores after 15 days under being the condition of L: D=9: 15, again temperature be 5-7 DEG C, humidity is 40-60%, periodicity of illumination carries out dormancy process, dormancy place after storing 25 days under being the condition of L: D=8-9: 15-16
reason is mainbe used for slowing down aphidius gifuensis to grow, improve aphidius gifuensis product memory time, ensure that the quality of product, meet aphidius gifuensis merchandized handling.
Beneficial effect of the present invention is: breeding method provided by the invention, shorten aphidius gifuensis breeding cycle, and aphidius gifuensis long term storage can be formed commercialization, for subsequent use at any time, convenient transport and sale, without the need to long-term cultivation, save human resources, reduce Financial cost, and can produce in enormous quantities, overcome that to there is the cycle in aphidius gifuensis reproductive process in prior art long, stiff aphid density is low, it is poor that stiff aphid forms regularity, stiff aphid collects difficulty, store transport difficult, the problems such as Field information difficulty, achieve the collection of aphidius gifuensis, store and transport formation integration, effective control aphid mycoses, for the production in enormous quantities of aphidius gifuensis, transport and sale create good condition, aphidius gifuensis is made to become a kind of commodity being convenient to circulate, be convenient to accumulate honeybee kind in a large number simultaneously, improve the availability of aphidius gifuensis, aphidius gifuensis the is possessed development trend of suitability for industrialized production.
Embodiment
Below by specific embodiment, the present invention is described in further detail.
Embodiment one: the industrialization breeding method of a kind of aphidius gifuensis described in the embodiment of the present invention, the method comprises the following steps:
The host plant of S101, cultivation cigarette aphid, and on host plant, breed cigarette aphid.
Further, the step of cultivating the host plant of cigarette aphid is:
1. leafy disease-resistant host plant seeding and seedling raising is selected, select tobacco, blue and white, radish divides 3 batches of seeding and seedling raisings, often criticize interval 15, first tobacco 50 strain, blue and white 60 strains, radish 50 strain, second batch tobacco 300 strain, blue and white 360 strains, radish 300 strain, the 3rd batch of tobacco 600 strain, blue and white 700 strains, radish 600 strain;
2. after first seedling strain seedling, select to transplant respectively strong sprout to temperature be 20 DEG C, humidity is 30%, possess 3 of fly net isolation breeds indoor, tobacco 3 Zhu/㎡, blue and white 5 Zhu/㎡, radish 4 Zhu/㎡; After second batch seedling strain seedling, select to transplant respectively strong sprout to temperature be 20 DEG C, humidity is 30%, breed indoor, tobacco 3 Zhu/㎡, blue and white 5 Zhu/㎡, radish 4 Zhu/㎡ with 18 of fly net isolation; After 3rd batch of seedling strain seedling, select to transplant respectively strong sprout to temperature be 20 DEG C, humidity is 30%, breed indoor, tobacco 3 Zhu/㎡, blue and white 5 Zhu/㎡, radish 4 Zhu/㎡ with 36 of fly net isolation; Then keep host plant water and fertilizer condition sufficient, cultivate under the condition of natural lighting, cultivate spray in a 20 days triazolone liquid, prevent and treat powdery mildew, finally obtain the host plant after cultivating.
Further, the step of host plant breeding cigarette aphid is:
1. aphid is connect: gather tobacco aphid kind and be evenly inoculated on first host plant cultivated, inoculum concentration is 5 heads/plant;
2. cigarette aphid breeds: by connecing, the host plant after aphid moves into that temperature is 20 DEG C, humidity is 30%, to possess numerous aphid of fly net isolation indoor, freely breeds 10 days, namely obtain the cigarette aphid after breeding under the condition of natural lighting.
S102, the host bred as aphidius gifuensis by the cigarette aphid bred, cigarette aphid breeds the stiff aphid of aphidius gifuensis.
Further, the step of cigarette aphid breeding stiff aphid is:
1. connecing honeybee: collection aphidius gifuensis worm kind is inoculated in numerous aphid indoor and breeds on first host plant of cigarette aphid, connecing honeybee amount according to honeybee aphid than being 1:50;
2. aphidius gifuensis worm kind is bred: by connecing, the immigration of the host plant after honeybee temperature is 20 DEG C, humidity is 30%, it is indoor to possess numerous honeybee of fly net isolation, freely breeds 15 days, namely obtain the aphidius gifuensis worm kind after breeding under the condition of natural lighting;
3. the stiff aphid of aphidius gifuensis breeds: gather first cigarette aphid bred and be inoculated in and breed on indoor second batch host plant, inoculum concentration 30 heads/plant.Keep host plant water and fertilizer condition sufficient, breed indoor temperature 20-30 DEG C, in humidity 30-70% natural lighting situation, breed 10 days, on plant, aphid density reaches more than 2/square centimeter aphid major parts when being in 2-3 age, collect aphidius gifuensis worm kind breed after aphidius gifuensis, and be inoculated in numerous aphid indoor than 1:0.5 breed on the second batch host plant of cigarette aphid according to hero is female, connect honeybee amount according to honeybee aphid than for 1:25;
4. expand numerous: be inoculated in connecing the second batch host plant after honeybee and breed on indoor 3rd batch of host plant, inoculum concentration 10 heads/plant.And the 3rd batch of host plant is moved into temperature is 20 DEG C, humidity is 30%, to possess the expansion honeybee of fly net isolation indoor, 10 days are bred under the condition of natural lighting, when on host plant blade 80% aphid parasitic, and 50% start to form stiff aphid in by the aphid of parasitism, namely complete stiff aphid and breed.
Further, carry out the stiff aphid Hui Fan of aphidius gifuensis while the stiff aphid bred is separated with host plant tobacco leaf, the steps include:
1. cut breeding with part by the aphid of parasitism and in aphid the second batch host plant blade becoming stiff aphid with scissors, and be neatly uprightly positioned in separator;
2. separator is placed on that temperature is 20 DEG C, humidity is in 30% environment 8 hours, aphid climbs in a large number on separator inwall, again separator is placed on that temperature is 28 DEG C, humidity is in 30% environment 2 hours, finally by the aphid in separator, parasitic aphid are inoculated in breed inoculation aphidius gifuensis host plant on continue to breed stiff aphid.
S103, breed after stiff aphid be separated with host plant blade and collect stiff aphid.Stiff aphid was separated and collected stiff aphid step with blade is: be with the host plant blade of stiff aphid to be placed in stiff aphid separator in the host plant blade of stiff for second batch band aphid and the 3rd batch respectively, host plant blade is washed away with the supercharging shower nozzle that hydraulic pressure is 0.35MPa, stiff aphid is separated with host plant blade, collects stiff aphid simultaneously.
S104, by after the stiff aphid sterilization after collecting, carry out air-dry process, use UV-irradiation process.
To the step of the stiff aphid process after collection be:
1. sterilize: second batch collect after stiff aphid and second batch collect after the stiff aphid hydrogen peroxide that is all placed in 1% disinfect 1 minute;
2. air-dry: the stiff aphid uniform monolayers after sterilization to be covered in sub-sieve, and sub-sieve to be put in air dryer air-dry;
3. ultra violet lamp sub-sieve is used 3 minutes.
S105, the inspection of stiff aphid and packing: to reject in first and second batch in spite of illness after damaged and stiff aphid that individuality is less, stiff aphid is moved in packing test tube or be evenly laid on paper card and make stiff aphid card.
S106, will the packing test tube of stiff aphid be accommodated or stiff aphid card stores, and pack according to demand.
The packing test tube or stiff aphid that accommodate stiff aphid are blocked the step carrying out storing is: first the band packing test tube of stiff aphid or stiff aphid are stuck in that temperature is 5 DEG C, periodicity of illumination is after storing 15 days under the condition of L: D=9: 15, then temperature be 5 DEG C, humidity is 40%, periodicity of illumination stores 25 days under being the condition of L: D=8: 15.
Finally, second batch and the 3rd batch are all carried out the packing of product, and the concrete title of labeling, controlling object, control area, using method, the use age, application notice, use ice bag Refrigerated Transport according to weather conditions, haulage time afterwards.
In the present embodiment, the cigarette aphid on first host plant is mainly used in cultivating aphidius gifuensis, and second batch and the 3rd batch of cigarette aphid finally breed stiff aphid.
The convenient transport of the present invention, shorten aphidius gifuensis breeding cycle, and aphidius gifuensis long term storage can be formed commercialization, for subsequent use at any time, convenient transport and sale, without the need to long-term cultivation, save human resources, reduce Financial cost, and can produce in enormous quantities.
Experiment one, use above-mentioned breeding method to produce the aphidius gifuensis obtained, eliminate aphis for anti-in vega, at the aphidius gifuensis using this breeding method to obtain over time, the quantity of aphid in test experience vega, detection obtain following data as
following table instituteshow:
Note: between vega, aphis population quantity adopts 5 sampling survey methods, often some investigation five strains.
Stiff aphid amount adopts 5 sampling survey methods, often some investigation five strain (stiff aphid refer to cigarette aphid laid eggs by aphidius gifuensis parasitism after aphid polypide dead, stiff, aphid polypide is opaque, polypide is inner aphidius gifuensis larva); Cigarette aphid parasitic rate adopts 5 sampling survey methods, often some investigation five strain (parasitic rate=(parasitic aphid+stiff aphid)/(aphid+parasitic aphid+stiff aphid body) × 100, parasitic aphid refer to cigarette aphid laid eggs by aphidius gifuensis parasitism after the cigarette aphid of body colour morphologic change).
Drawn by above-mentioned data: the aphidius gifuensis produced by the present invention eliminates aphis for anti-in vega, and it is better to the control effect of cigarette aphid, has higher lethality rate.
Experiment two, the aphidius gifuensis that uses above-mentioned breeding method to obtain, eliminate aphis for anti-in vega, at the aphidius gifuensis using this breeding method to obtain over time, the quantity of aphid in test experience vega, detect obtain data as
following table instituteshow:
Note: between vega, aphis population quantity adopts 5 sampling survey methods, often some investigation five strains.
Stiff aphid amount adopts 5 sampling survey methods, often some investigation five strain (stiff aphid refer to cigarette aphid laid eggs by aphidius gifuensis parasitism after aphid polypide dead, stiff, aphid polypide is opaque, polypide is inner aphidius gifuensis larva); Cigarette aphid parasitic rate adopts 5 sampling survey methods, often some investigation five strain (parasitic rate=(parasitic aphid+stiff aphid)/(aphid+parasitic aphid+stiff aphid body) × 100, parasitic aphid refer to cigarette aphid laid eggs by aphidius gifuensis parasitism after the cigarette aphid of body colour morphologic change).
Drawn by above-mentioned data: the stiff aphid burst size of different aphidius gifuensis is different, and they are also different to the control effect of cigarette aphid, and the aphidius gifuensis that the present invention produces has the effect of higher control cigarette aphid, effectively prevents and treats aphid mycoses.
Embodiment two: the industrialization breeding method of a kind of aphidius gifuensis described in the embodiment of the present invention, the method comprises the following steps:
The host plant of S101, cultivation cigarette aphid, and on host plant, breed cigarette aphid.
Further, the step of cultivating the host plant of cigarette aphid is:
1. select leafy disease-resistant host plant seeding and seedling raising, selection green vegetables, water spinach divide 3 batches of seeding and seedling raisings, often criticize interval 15, first green vegetables 90 strain, water spinach 160 strain; Second batch green vegetables 500 strain, water spinach 400 strain; 3rd batch of green vegetables 1000 strain, water spinach 800 strain.
2. after first seedling strain seedling, select to transplant respectively strong sprout to temperature be 25 DEG C, humidity is 50%, possess 2 of fly net isolation breeds indoor, green vegetables 4 Zhu/㎡, water spinach 10 Zhu/㎡; After second batch seedling strain seedling, select to transplant respectively strong sprout to temperature be 25 DEG C, humidity is 50%, breed indoor, green vegetables 4 Zhu/㎡, water spinach 10 Zhu/㎡ with 18 of fly net isolation; After 3rd batch of seedling strain seedling, select to transplant respectively strong sprout to temperature be 25 DEG C, humidity is 50%, breed indoor, green vegetables 4 Zhu/㎡, water spinach 10 Zhu/㎡ with 36 of fly net isolation; Then keep host plant water and fertilizer condition sufficient, cultivate under the condition of natural lighting, cultivate spray in a 20 days triazolone liquid, prevent and treat powdery mildew, finally obtain the host plant after cultivating.
Further, the step of host plant breeding cigarette aphid is:
1. aphid is connect: gather tobacco aphid kind and be evenly inoculated on first host plant cultivated, inoculum concentration is 8 heads/plant;
2. cigarette aphid breeds: by connecing, the host plant after aphid moves into that temperature is 25 DEG C, humidity is 50%, to possess numerous aphid of fly net isolation indoor, freely breeds 10 days, namely obtain the cigarette aphid after breeding under the condition of natural lighting.
S102, the host bred as aphidius gifuensis by the cigarette aphid bred, cigarette aphid breeds the stiff aphid of aphidius gifuensis.
Further, the step of cigarette aphid breeding stiff aphid is:
1. connecing honeybee: collection aphidius gifuensis worm kind is inoculated in numerous aphid indoor and breeds on first host plant of cigarette aphid, connecing honeybee amount according to honeybee aphid than being 1:80;
2. aphidius gifuensis worm kind is bred: by connecing, the immigration of the host plant after honeybee temperature is 25 DEG C, humidity is 50%, it is indoor to possess numerous honeybee of fly net isolation, freely breeds 15 days, namely obtain the aphidius gifuensis worm kind after breeding under the condition of natural lighting;
3. the stiff aphid of aphidius gifuensis breeds: gather first cigarette aphid bred and be inoculated in and breed on indoor second batch host plant, inoculum concentration 30 heads/plant.Keep host plant water and fertilizer condition sufficient, under breeding indoor temperature 25 DEG C, humidity 50% natural lighting situation, breed 10 days, on plant, aphid density reaches more than 2/square centimeter aphid major parts when being in for 3 age, collect aphidius gifuensis worm kind breed after aphidius gifuensis, and be inoculated in numerous aphid indoor than 1:0.8 breed on the second batch host plant of cigarette aphid according to hero is female, connect honeybee amount according to honeybee aphid than for 1:40;
4. expand numerous: be inoculated in connecing the second batch host plant after honeybee and breed on indoor 3rd batch of host plant, inoculum concentration 10 heads/plant.And the 3rd batch of host plant is moved into temperature is 25 DEG C, humidity is 50%, to possess the expansion honeybee of fly net isolation indoor, 10 days are bred under the condition of natural lighting, when on host plant blade 80% aphid parasitic, and 50% start to form stiff aphid in by the aphid of parasitism, namely complete stiff aphid and breed.
Further, carry out the stiff aphid Hui Fan of aphidius gifuensis while the stiff aphid bred is separated with host plant tobacco leaf, the steps include:
1. cut breeding with part by the aphid of parasitism and in aphid the second batch host plant blade becoming stiff aphid with scissors, and be neatly uprightly positioned in separator;
2. separator is placed on that temperature is 25 DEG C, humidity is in 50% environment 10 hours, aphid climbs in a large number on separator inwall, again separator is placed on that temperature is 29 DEG C, humidity is in 50% environment 3 hours, finally by the aphid in separator, parasitic aphid are inoculated in breed inoculation aphidius gifuensis host plant on continue to breed stiff aphid.
S103, breed after stiff aphid be separated with host plant blade, and collect stiff aphid.Stiff aphid was separated and collected stiff aphid step with blade is: be with the host plant blade of stiff aphid to be placed in stiff aphid separator in the host plant blade of stiff for second batch band aphid and the 3rd batch respectively, host plant blade is washed away with the supercharging shower nozzle that hydraulic pressure is 0.42MPa, stiff aphid is separated with host plant blade, collects stiff aphid simultaneously.
S104, by after the stiff aphid sterilization after collecting, carry out air-dry process, use UV-irradiation process.
To the step of the stiff aphid process after collection be:
1. sterilize: second batch collect after stiff aphid and second batch collect after the stiff aphid hydrogen peroxide that is all placed in 2% disinfect 1.5 minutes;
2. air-dry: the stiff aphid uniform monolayers after sterilization to be covered in sub-sieve, and sub-sieve to be put in air dryer air-dry;
3. ultra violet lamp sub-sieve is used 4 minutes.
S105, the inspection of stiff aphid and packing: to reject in first and second batch in spite of illness after damaged and stiff aphid that individuality is less, stiff aphid is moved in packing test tube or be evenly laid on paper card and make stiff aphid card.
S106, will the packing test tube of stiff aphid be accommodated or stiff aphid card stores, and pack according to demand.
The packing test tube or stiff aphid that accommodate stiff aphid are blocked the step carrying out storing is: first the band packing test tube of stiff aphid or stiff aphid are stuck in that temperature is 5 DEG C, periodicity of illumination is after storing 15 days under the condition of L: D=9: 15, then temperature be 6 DEG C, humidity is 50%, periodicity of illumination stores 15 days under being the condition of L: D=8.5: 15.5.
Finally, second batch and the 3rd batch are all carried out the packing of product, and the concrete title of labeling, controlling object, control area, using method, the use age, application notice, use ice bag Refrigerated Transport according to weather conditions, haulage time afterwards.
In the present embodiment, the cigarette aphid on first host plant is mainly used in cultivating aphidius gifuensis, and second batch and the 3rd batch of cigarette aphid finally breed stiff aphid.
The present invention can produce in batches, improves aphidius gifuensis production efficiency, makes aphidius gifuensis become a kind of commodity being convenient to circulate, is convenient to accumulate honeybee kind in a large number simultaneously, improves the availability of aphidius gifuensis.
Experiment three, use above-mentioned breeding method to produce the aphidius gifuensis obtained, for plant Chinese cabbage in booth field in anti-ly to eliminate aphis, at the aphidius gifuensis using this breeding method to obtain over time, the quantity of aphid in test experience field, detection obtain data as
following table instituteshow:
Note: between vega, aphis population quantity adopts 5 sampling survey methods, often some investigation five strains.
Stiff aphid amount adopts 5 sampling survey methods, often some investigation five strain (stiff aphid refer to cigarette aphid laid eggs by aphidius gifuensis parasitism after aphid polypide dead, stiff, aphid polypide is opaque, polypide is inner aphidius gifuensis larva); Cigarette aphid parasitic rate adopts 5 sampling survey methods, often some investigation five strain (parasitic rate=(parasitic aphid+stiff aphid)/(aphid+parasitic aphid+stiff aphid body) × 100, parasitic aphid refer to cigarette aphid laid eggs by aphidius gifuensis parasitism after the cigarette aphid of body colour morphologic change).
Drawn by above-mentioned data: it is better to the control effect of aphid in booth that method provided by the invention cultivates the aphidius gifuensis produced, and make the aphid in booth have higher lethality rate, and control effect is better.
Experiment four, the aphidius gifuensis that uses above-mentioned breeding method to obtain, for plant Chinese cabbage in booth field in anti-ly to eliminate aphis, at the aphidius gifuensis using this breeding method to obtain over time, the quantity of aphid in test experience field, detect and obtain following data:
Note: between vega, aphis population quantity adopts 5 sampling survey methods, often some investigation five strains.
Stiff aphid amount adopts 5 sampling survey methods, often some investigation five strain (stiff aphid refer to cigarette aphid laid eggs by aphidius gifuensis parasitism after aphid polypide dead, stiff, aphid polypide is opaque, polypide is inner aphidius gifuensis larva); Cigarette aphid parasitic rate adopts 5 sampling survey methods, often some investigation five strain (parasitic rate=(parasitic aphid+stiff aphid)/(aphid+parasitic aphid+stiff aphid body) × 100, parasitic aphid refer to cigarette aphid laid eggs by aphidius gifuensis parasitism after the cigarette aphid of body colour morphologic change).
Drawn by above-mentioned data: the burst size of the aphidius gifuensis that the present invention produces improves further to the control effect of aphid in booth, result shows that the aphidius gifuensis that the present invention produces has good control effect to the aphid in booth, the aphidius gifuensis that the present invention produces can have control effect for the aphid occurred in booth vegetable process, therefore the present invention can be widely applied to booth production base, for prevention and control aphid, be convenient to promote and transport.
Embodiment three: the industrialization breeding method of a kind of aphidius gifuensis described in the embodiment of the present invention, the method comprises the following steps:
The host plant of S101, cultivation cigarette aphid, and on host plant, breed cigarette aphid.
Further, the step of cultivating the host plant of cigarette aphid is:
1. select leafy disease-resistant host plant seeding and seedling raising, selection tobacco, radish divide 3 batches of seeding and seedling raisings, often criticize interval 15, first tobacco 80 strain, radish 100 strain; Second batch tobacco 400 strain, radish 300 strain; 3rd batch of tobacco 800 strain, radish 600 strain.
2. after first seedling strain seedling, select to transplant respectively strong sprout to temperature be 30 DEG C, humidity is 70%, possess 2 of fly net isolation breeds indoor, tobacco 4 Zhu/㎡, radish 10 Zhu/㎡; After second batch seedling strain seedling, select to transplant respectively strong sprout to temperature be 30 DEG C, humidity is 70%, breed indoor, tobacco 4 Zhu/㎡, radish 10 Zhu/㎡ with 18 of fly net isolation; After 3rd batch of seedling strain seedling, select to transplant respectively strong sprout to temperature be 30 DEG C, humidity is 70%, breed indoor, tobacco 4 Zhu/㎡, radish 10 Zhu/㎡ with 36 of fly net isolation; Then keep host plant water and fertilizer condition sufficient, cultivate under the condition of natural lighting, cultivate spray in a 20 days triazolone liquid, prevent and treat powdery mildew, finally obtain the host plant after cultivating.
Further, the step of host plant breeding cigarette aphid is:
1. aphid is connect: gather tobacco aphid kind and be evenly inoculated on first host plant cultivated, inoculum concentration is 10 heads/plant;
2. cigarette aphid breeds: by connecing, the host plant after aphid moves into that temperature is 30 DEG C, humidity is 70%, to possess numerous aphid of fly net isolation indoor, freely breeds 15 days, namely obtain the cigarette aphid after breeding under the condition of natural lighting.
S102, the host bred as aphidius gifuensis by the cigarette aphid bred, cigarette aphid breeds the stiff aphid of aphidius gifuensis.
Further, the step of cigarette aphid breeding stiff aphid is:
1. connecing honeybee: collection aphidius gifuensis worm kind is inoculated in numerous aphid indoor and breeds on first host plant of cigarette aphid, connecing honeybee amount according to honeybee aphid than being 1:100;
2. aphidius gifuensis worm kind is bred: by connecing, the immigration of the host plant after honeybee temperature is 30 DEG C, humidity is 70%, it is indoor to possess numerous honeybee of fly net isolation, freely breeds 15 days, namely obtain the aphidius gifuensis worm kind after breeding under the condition of natural lighting;
3. the stiff aphid of aphidius gifuensis breeds: gather first cigarette aphid bred and be inoculated in and breed on indoor second batch host plant, inoculum concentration 30 heads/plant.Keep host plant water and fertilizer condition sufficient, under breeding indoor temperature 30 DEG C, humidity 70% natural lighting situation, breed 10 days, on plant, aphid density reaches more than 2/square centimeter aphid major parts when being in for 3 age, collect aphidius gifuensis worm kind breed after aphidius gifuensis, and be inoculated in numerous aphid indoor than 1:1.0 breed on the second batch host plant of cigarette aphid according to hero is female, connect honeybee amount according to honeybee aphid than for 1:50;
4. expand numerous: be inoculated in connecing the second batch host plant after honeybee and breed on indoor 3rd batch of host plant, inoculum concentration 10 heads/plant.And the 3rd batch of host plant is moved into temperature is 30 DEG C, humidity is 70%, to possess the expansion honeybee of fly net isolation indoor, 10 days are bred under the condition of natural lighting, when on host plant blade 80% aphid parasitic, and 50% start to form stiff aphid in by the aphid of parasitism, namely complete stiff aphid and breed.
Further, carry out the stiff aphid Hui Fan of aphidius gifuensis while the stiff aphid bred is separated with host plant tobacco leaf, the steps include:
1. cut breeding with part by the aphid of parasitism and in aphid the second batch host plant blade becoming stiff aphid with scissors, and be neatly uprightly positioned in separator;
2. separator is placed on that temperature is 30 DEG C, humidity is in 70% environment 12 hours, aphid climbs in a large number on separator inwall, again separator is placed on that temperature is 30 DEG C, humidity is in 70% environment 4 hours, finally by the aphid in separator, parasitic aphid are inoculated in breed inoculation aphidius gifuensis host plant on continue to breed stiff aphid.
S103, breed after stiff aphid be separated with host plant blade, and collect stiff aphid.Stiff aphid was separated and collected stiff aphid step with blade is: be with the host plant blade of stiff aphid to be placed in stiff aphid separator in the host plant blade of stiff for second batch band aphid and the 3rd batch respectively, host plant blade is washed away with the supercharging shower nozzle that hydraulic pressure is 0.5MPa, stiff aphid is separated with host plant blade, collects stiff aphid simultaneously.
S104, by after the stiff aphid sterilization after collecting, carry out air-dry process, use UV-irradiation process.
To the step of the stiff aphid process after collection be:
1. sterilize: second batch collect after stiff aphid and second batch collect after the stiff aphid hydrogen peroxide that is all placed in 2% disinfect 2 minutes;
2. air-dry: the stiff aphid uniform monolayers after sterilization to be covered in sub-sieve, and sub-sieve to be put in air dryer air-dry;
3. ultra violet lamp sub-sieve is used 5 minutes.
S105, the inspection of stiff aphid and packing: to reject in first and second batch in spite of illness after damaged and stiff aphid that individuality is less, stiff aphid is moved in packing test tube or be evenly laid on paper card and make stiff aphid card.
S106, will the packing test tube of stiff aphid be accommodated or stiff aphid card stores, and pack according to demand.
The packing test tube or stiff aphid that accommodate stiff aphid are blocked the step carrying out storing is: first the band packing test tube of stiff aphid or stiff aphid are stuck in that temperature is 5 DEG C, periodicity of illumination is after storing 15 days under the condition of L: D=9: 15, then temperature be 7 DEG C, humidity is 60%, periodicity of illumination stores 20 days under being the condition of L: D=9: 16.
Finally, second batch and the 3rd batch are all carried out the packing of product, and the concrete title of labeling, controlling object, control area, using method, the use age, application notice, use ice bag Refrigerated Transport according to weather conditions, haulage time afterwards.
In the present embodiment, the cigarette aphid on first host plant is mainly used in cultivating aphidius gifuensis, and second batch and the 3rd batch of cigarette aphid finally breed stiff aphid.
Test five, use above-mentioned breeding method to produce the aphidius gifuensis obtained, for plant Chinese cabbage in booth field in anti-ly to eliminate aphis, at the aphidius gifuensis using this breeding method to obtain over time, the quantity of aphid in test experience field, detects and obtains following data:
Note: between vega, aphis population quantity adopts 5 sampling survey methods, often some investigation five strains.
Stiff aphid amount adopts 5 sampling survey methods, often some investigation five strain (stiff aphid refer to cigarette aphid laid eggs by aphidius gifuensis parasitism after aphid polypide dead, stiff, aphid polypide is opaque, polypide is inner aphidius gifuensis larva); Cigarette aphid parasitic rate adopts 5 sampling survey methods, often some investigation five strain (parasitic rate=(parasitic aphid+stiff aphid)/(aphid+parasitic aphid+stiff aphid body) × 100, parasitic aphid refer to cigarette aphid laid eggs by aphidius gifuensis parasitism after the cigarette aphid of body colour morphologic change).
Drawn by above-mentioned data: the aphidius gifuensis that this experimental verification the present invention produces is in the control efficiency in different application period, and result shows that application aphidius gifuensis early carries out prevention and control to aphid, control effect is better.
In sum, from above-described embodiment and test, aphidius gifuensis is to the direct control effects of cigarette aphid: can reduce cigarette aphid population quantity in time; After the parasitic cigarette aphid of aphidius gifuensis, average farrowing rate reduces more than 95%, and 13.6d is shortened in mean lifetime, and land for growing field crops bulk storage aphidius gifuensis, the quantity that effectively can control cigarette aphid population increases.
Aphidius gifuensis can play lasting control effects to cigarette aphid: the control effects time of aphidius gifuensis to cigarette aphid is longer; Carry out Field release, aphidius gifuensis has been grown putting honeybee region surely, and maintains higher population quantity, and occurring in nature aphidius gifuensis population quantity increases considerably for years.Aphidius gifuensis is worldwide insect, also extensively distributes in China., there is not many ecological problems such as foreign term, environmental pollution, residue of pesticide in artificial release aphidius gifuensis control cigarette aphid.Long-term release aphidius gifuensis, the Natural Enemies kind of cigarette aphid and quantity increase to some extent, are not only conducive to bio-diversity conservation, also can improve the Natural control action of field ecosystem.Application aphidius gifuensis control cigarette aphid technology, every mu is reduced cost accounting more than 20 yuan.By implementing the biological prevention of aphidius gifuensis control cigarette aphid for a long time, decreasing Pesticide use amount and residue of pesticide, and then quality and the market competitiveness of flue-cured tobacco can be improved, therefore there is very large potential economic benefit.
Aphidius gifuensis not only has good control effects to the aphid on flue-cured tobacco, simultaneously has good control effects in the region of continuous release aphidius gifuensis equally to the aphid above rape, fruit tree, vegetables, flowers etc., accept by numerous tobacco growers.This measure decreases the Productive statistics of tobacco grower; and add income, reduce the residual usage amount of agricultural chemicals, to protecting the health of Ecological Environment and tobacco grower and consumer, promoting that the development of society and economy is significant; there is very large social benefit, improve tobacco positive energy.
The industrialization breeding method of aphidius gifuensis provided by the invention, shorten the time that aphidius gifuensis is bred, the transport of aphidius gifuensis and storage is made to become possibility, pass through the present invention, user can utilize aphidius gifuensis to prevent and treat greenhouse at any time, the aphid in orchard and land for growing field crops, this controlling mode will advance the industrialized development process discharging the biological prevention that natural enemy insect is main body, not only can reduce the usage amount of chemical pesticide, alleviate pest resistance to insecticide development, reduce residue of pesticide, ensure food security and consumer's interests, simultaneously can biometric safeguard diversity and making full use of the natural resources, the merchandized handling of natural enemy insect is as an emerging scientific and technological industry, have broad application prospects, larger contribution will be made for the safety in production of the agricultural product of China.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for a person skilled in the art, the present invention can have various modifications and variations.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (8)
1. an industrialization breeding method for aphidius gifuensis, it is characterized in that, the method comprises the following steps:
The host plant of S101, cultivation cigarette aphid, and on host plant, breed cigarette aphid;
S102, the host bred as aphidius gifuensis by the cigarette aphid bred, cigarette aphid breeds the stiff aphid of aphidius gifuensis;
S103, breed after stiff aphid be separated with host plant blade and collect stiff aphid;
S104, by after the stiff aphid sterilization after collecting, carry out air-dry process, finally use UV-irradiation process;
S105, the inspection of stiff aphid and packing: after rejecting the stiff aphid that damaged and individuality is less in spite of illness, move into stiff aphid in packing test tube or be evenly laid in and paper card makes stiff aphid block;
S106, will the packing test tube of stiff aphid be accommodated or stiff aphid card stores, and pack according to demand.
2. the industrialization breeding method of aphidius gifuensis according to claim 1, is characterized in that, in step S101, the step of cultivating the host plant of cigarette aphid is:
1. leafy disease-resistant host plant seeding and seedling raising is selected;
2. transplant after seedling strain seedling to temperature be 20-30 DEG C, humidity is 30-70%, possess fly net isolation breed indoor, cultivate under the condition of natural lighting.
3. the industrialization breeding method of aphidius gifuensis according to claim 2, is characterized in that, the step of breeding cigarette aphid in step S101 is:
1. aphid is connect: gather tobacco aphid kind and evenly inoculate with on the host plant cultivated, inoculum concentration is 5-10 head/plant;
2. cigarette aphid breeds: by connecing, the host plant after aphid moves into that temperature is 20-30 DEG C, humidity is 30-70%, to possess numerous aphid of fly net isolation indoor, freely breeds 10-15 days, namely obtain the cigarette aphid after breeding under the condition of natural lighting.
4. the industrialization breeding method of aphidius gifuensis according to claim 1, is characterized in that, in step S102, the step that cigarette aphid breeds stiff aphid is:
1. connecing honeybee: collection aphidius gifuensis worm kind is inoculated in numerous aphid indoor section and breeds on the host plant of cigarette aphid, connecing honeybee amount according to honeybee aphid than being 1:50-100;
2. aphidius gifuensis worm kind is bred: by connecing, the immigration of the host plant after honeybee temperature is 20-30 DEG C, humidity is 30-70%, it is indoor to possess numerous honeybee of fly net isolation, freely breeds 15 days, namely obtain the aphidius gifuensis worm kind after breeding under the condition of natural lighting;
3. the stiff aphid of aphidius gifuensis breeds: collect aphidius gifuensis worm kind breed after aphidius gifuensis, and being inoculated in numerous aphid indoor than 1:0.5-1.0 breeding on the host plant of cigarette aphid according to hero be female, connecing honeybee amount according to honeybee aphid than being 1:25-50;
4. expand numerous: by connecing, the host plant after honeybee moves into that temperature is 20-30 DEG C, humidity is 30-70%, to possess the expansion honeybee of fly net isolation indoor, 10 days are bred under the condition of natural lighting, when on host plant blade 80% aphid parasitic, and 50% start to form stiff aphid in by the aphid of parasitism, namely complete stiff aphid and breed.
5. the industrialization breeding method of aphidius gifuensis according to claim 1, it is characterized in that, in step S103, stiff aphid with the step that host plant tobacco leaf was separated and collected stiff aphid is: be placed in stiff aphid separator by being with the host plant blade of stiff aphid, host plant blade is washed away with the supercharging shower nozzle that hydraulic pressure is 0.35MPa-0.50MPa, stiff aphid is separated with host plant blade, collects stiff aphid simultaneously.
6. the industrialization breeding method of aphidius gifuensis according to claim 1, is characterized in that, carries out stiff aphid Hui Fan, the steps include: while carrying out step S103
1. cut breeding with part by the aphid of parasitism and in aphid the host plant blade becoming stiff aphid with scissors, and be positioned in separator;
2. separator is placed on that temperature is 20-30 DEG C, humidity is 8-12 hour in 30-70% environment, aphid, parasitic aphid climb in a large number on separator inwall, again separator is placed on that temperature is 28-30 DEG C, humidity is 2-4 hour in 30-70% environment, finally by the aphid in separator, parasitic aphid are inoculated in breed inoculation aphidius gifuensis host plant on continue to breed stiff aphid.
7. the industrialization breeding method of aphidius gifuensis according to claim 1, is characterized in that, in step S104, by after collecting stiff aphid carries out disinfection, step that is air-dry and UV-irradiation is:
1. sterilize: the stiff aphid after collection is placed in hydrogen peroxide and disinfects 1-2 minute;
2. air-dry: the stiff aphid uniform monolayers after sterilization to be covered in sub-sieve, and sub-sieve to be put in air dryer air-dry;
3. ultra violet lamp sub-sieve 3-5 minute is used.
8. the industrialization breeding method of aphidius gifuensis according to claim 1, it is characterized in that, in step S106, the band packing test tube of stiff aphid or stiff aphid blocks the step carrying out storing is: first the packing test tube of the stiff aphid of band or stiff aphid are stuck in that temperature is 5 DEG C, periodicity of illumination is after storing 15 days under the condition of L: D=9: 15, then temperature be 5-7 DEG C, humidity is 40-60%, periodicity of illumination be the condition of L: D=8-9: 15-16 under storage 25 days.
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| CN108935337B (en) * | 2018-06-26 | 2021-04-02 | 江苏省林业科学研究院 | Technology for raising breeding success rate and breeding number of scleroderma guani |
| CN109197782A (en) * | 2018-08-08 | 2019-01-15 | 云南省烟草公司玉溪市公司 | A kind of the selecting quantitative separating and storage method for releasing of deadlock aphid |
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| CN109221022B (en) * | 2018-10-29 | 2023-09-26 | 云南省烟草公司昆明市公司 | Automatic aphidiidae product production device |
| CN110122429A (en) * | 2019-06-18 | 2019-08-16 | 河南省农业科学院烟草研究所 | A kind of aphid parasite deadlock aphid preservation test method |
| CN110122429B (en) * | 2019-06-18 | 2021-07-02 | 河南省农业科学院烟草研究所 | Stiff aphid preservation test method for aphidiidae |
| CN113040095A (en) * | 2019-12-27 | 2021-06-29 | 云南格瑞生物科技有限公司 | Method for separating and purifying stiff aphids of aphidiidae |
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| CN112931423A (en) * | 2021-03-19 | 2021-06-11 | 湖南省烟草公司衡阳市公司常宁市分公司 | Method for collecting stiff aphids of aphidius gifuensis in experimental research |
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