CN107624720A - A kind of aphid method for breeding and its application suitable for aphidius gifuensis breeding - Google Patents
A kind of aphid method for breeding and its application suitable for aphidius gifuensis breeding Download PDFInfo
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- CN107624720A CN107624720A CN201711079987.0A CN201711079987A CN107624720A CN 107624720 A CN107624720 A CN 107624720A CN 201711079987 A CN201711079987 A CN 201711079987A CN 107624720 A CN107624720 A CN 107624720A
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Abstract
The invention belongs to insect artificial propagation techniques field, and in particular to a kind of aphid method for breeding suitable for aphidius gifuensis breeding, and the anniversary artificial fecundation method using this method progress aphidius gifuensis.Cigarette aphid method for breeding of the present invention suitable for aphidius gifuensis breeding, by controlling the parameters in aphid growth course, control photoperiod L10 16：D8 14, the 5000lx of intensity of illumination 3000 so that larvae aphid survival rate height, adult aphid life-span and the breeding period length bred, its average reproductive capacity are higher.
Description
Technical field
The invention belongs to insect artificial propagation techniques field, and in particular to a kind of aphid raising suitable for aphidius gifuensis breeding
Method, and the anniversary artificial fecundation method using this method progress aphidius gifuensis.
Background technology
It is reported that endangering thousands of kinds of the disease pest species of tobacco in world wide, have because chronic administration chemical pesticide shows oneself
More than 600 kinds of disease pest is developed immunity to drugs, and causes chemical pesticide dosage increasing, and a large amount of natural enemy insects are killed, and not only ecology is flat
Weighing apparatus is destroyed, and Minor diseases rise to main diseases and insect pests, and the disease pest extent of injury is on the rise.Wherein, cigarette aphid is to endanger tobacco
One of primary pest, in 21 generations, occurred for annual, without obvious diapause phenomenon of surviving the winter, the normal growth and development of cigarette strain is produced compared with
Big negative effect, and " dark mildew " easily is induced, directly affect the yield and quality of tobacco leaf；Meanwhile cigarette aphid or a variety of diseases
The biography poison medium of viral disease, cause a variety of tobacco virus and worm to pass the prevalence of tobacco diseases, cause yield of tobacco Quality Down, it is right
Cured tobacco production causes very big loss.
Aphidius gifuensis Aphidiusgifuensis (Ashmead), it is a kind of internal excited state of special parasitic aphid, to posting
The natural contral power of main aphid is stronger, main parasitic cigarette aphid (black peach aphid) Myzuspersicae, radish aphid Lipaphis erysimi,
Tobacco and the vegetables such as small grain aphid Macrosiphum avenae, cotten aphid Aphisgossypii, soybean aphid Aphisglycines
Dish crop aphid, is the Important Natural Enemy of cigarette aphid, and the biological prevention and control for cigarette aphid have the function that important.
Aphidius gifuensis, it is Hymenoptera, Aphidiidae, is distributed mainly on China, Korea, Japan, the U.S., Canada etc., its
Host insect mainly has green bugs, grain aphid, cotten aphid, soybean aphid, black peach aphid etc., main harm wheat, cotton, soybean, oil
Dish, wild cabbage, Chinese cabbage etc. crops.
Use using cigarette aphid MyzusPesricae (Sulzer) is host, using tobacco as vector plant more in the prior art
Mode expands numerous aphidius gifuensis.However, on the one hand because the aphid growth for breeding aphidius gifuensis is limited and aphid by external environmental condition
The parasitic effects of chalcid fly, and aphid is more sensitive to light application ratio, and illumination not only influences the spatial and temporal distributions, Sex Differentiation, wing of aphid
Type differentiation and body-color diversity etc., and Development and reproduction to aphid etc. can have an impact so that in actual production
The scale annual for being difficult to cigarette aphid is bred, and causes that the annual of aphidius gifuensis is bred and yield is also greatly affected.
The content of the invention
Therefore, the technical problems to be solved by the invention are to provide a kind of aphid raising side suitable for aphidius gifuensis breeding
Method, to solve the problems, such as that the scale annual amount of breeding of cigarette aphid in the prior art is relatively low.
In order to solve the above technical problems, a kind of aphid method for breeding suitable for aphidius gifuensis breeding of the present invention, bag
Include following steps：
(1) tobacco seedlings are planted：Tobacco seedlings are conventionally planted in glasshouse, after going out true leaf to tobacco seedling growth, by institute
State tobacco seedlings and move into climatic chamber, it is standby；
(2) aphid is connect：Tobacco leaf with cigarette aphid is put into the tobacco seedlings of transplanting, completes aphid inoculation；
(3) 24-26 DEG C, humidity 60%-70%, photoperiod L10-16 of weather indoor temperature is controlled：D8-14, intensity of illumination
3000-5000lx, carry out breeding for aphid.
In the step (1), the tobacco seedlings are to grow at least 6 true leaves.
In the step (2), after the aphid inoculation step, the step of old blade is removed after being also included in one day.
In the step (3), in addition to after aphid grow 8-12 days after be inoculated with aphid again the step of.
In the step (3), it is additionally included in climatic chamber and places the step of growth promotes liquid.
In the step (3), it is the maltitol solution that mass concentration is 5-10wt% that the growth, which promotes liquid,.
The growth promotes liquid also to include the glutamine that mass concentration is 1-2wt%.
The invention also discloses the described aphid method for breeding for being suitable to aphidius gifuensis breeding is artificial in the aphidius gifuensis anniversary
The application of breeding field.
Enter the invention also discloses a kind of anniversary artificial fecundation method of aphidius gifuensis, including according to described method for breeding
The step of row cigarette aphid breeds, and the step of inoculation aphidius gifuensis.
In the inoculation step, the benefit evil of aphidius gifuensis is than being 1：50-60.
Cigarette aphid method for breeding of the present invention suitable for aphidius gifuensis breeding, by controlling the items in aphid growth course
Parameter, control photoperiod L10-16：D8-14, intensity of illumination 3000-5000lx so that the larvae aphid survival rate bred is high, the adult aphid longevity
Life and breeding period length, its average reproductive capacity are higher；The present invention it is more excellent breed in workshop set by maltitol and glutamy
The growth of amine composition promotes liquid, and to promote the growth of tobacco aphid, the culture and anniversary for being more beneficial for aphid are bred.Beneficial to the present invention
The maize aphidses method for breeding, which can be bred, obtains substantial amounts of High Quality Tobacco aphid, for aphidius gifuensis breed in anniversary provide it is favourable
Condition.
Embodiment
Embodiment 1
The aphid method for breeding for being suitable to aphidius gifuensis breeding described in the present embodiment, comprises the following steps：
(1) tobacco seedlings are planted：Tobacco seedlings are conventionally planted in glasshouse, to tobacco seedling growth to 6 true leaves after, will
The tobacco seedlings move into climatic chamber, standby；
(2) aphid is connect：Tobacco leaf with cigarette aphid is put into the tobacco seedlings of transplanting, completes aphid inoculation；And in connecing
Kind removed old blade after one day；
(3) 24-26 DEG C, humidity 60%-70%, photoperiod L10 of weather indoor temperature is controlled：D14, intensity of illumination
3000lx, breeding for aphid is carried out, while aphid larva is inoculated with again after aphid grows 10 days or so, treat that aphid density reaches
During per 20, blade, available for aphidius gifuensis inoculation and breed.
Embodiment 2
The aphid method for breeding for being suitable to aphidius gifuensis breeding described in the present embodiment, comprises the following steps：
(1) tobacco seedlings are planted：Tobacco seedlings are conventionally planted in glasshouse, to tobacco seedling growth to 6 true leaves after, will
The tobacco seedlings move into climatic chamber, standby；
(2) aphid is connect：Tobacco leaf with cigarette aphid is put into the tobacco seedlings of transplanting, completes aphid inoculation；And in connecing
Kind removed old blade after one day；
(3) 24-26 DEG C, humidity 60%-70%, photoperiod L13 of weather indoor temperature is controlled：D11, intensity of illumination
5000lx, breeding for aphid is carried out, while aphid larva is inoculated with again after aphid grows 10 days or so, treat that aphid density reaches
During per 20, blade, available for aphidius gifuensis inoculation and breed.
Embodiment 3
The aphid method for breeding for being suitable to aphidius gifuensis breeding described in the present embodiment, comprises the following steps：
(1) tobacco seedlings are planted：Tobacco seedlings are conventionally planted in glasshouse, to tobacco seedling growth to 6 true leaves after, will
The tobacco seedlings move into climatic chamber, standby；
(2) aphid is connect：Tobacco leaf with cigarette aphid is put into the tobacco seedlings of transplanting, completes aphid inoculation；And in connecing
Kind removed old blade after one day；
(3) 24-26 DEG C, humidity 60%-70%, photoperiod L16 of weather indoor temperature is controlled：D8, intensity of illumination 5000lx,
Breeding for aphid is carried out, while aphid larva is inoculated with again after aphid grows 10 days or so, treats that aphid density reaches every blade
At 20, available for aphidius gifuensis inoculation and breed.
Embodiment 4
With embodiment 3, it is differed only in the tobacco aphid method for breeding for being suitable to aphidius gifuensis breeding described in the present embodiment,
In the step (3), it is additionally included in climatic chamber and places the step of growth promotes liquid, it is that mass concentration is that the growth, which promotes liquid,
5wt% maltitol solution.
Embodiment 5
With embodiment 3, it is differed only in the tobacco aphid method for breeding for being suitable to aphidius gifuensis breeding described in the present embodiment,
In the step (3), it is additionally included in climatic chamber and places the step of growth promotes liquid, it is that mass concentration is that the growth, which promotes liquid,
10wt% maltitol solution.
Embodiment 6
With embodiment 3, it is differed only in the tobacco aphid method for breeding for being suitable to aphidius gifuensis breeding described in the present embodiment,
The growth promotes liquid also to include the glutamine solution that mass concentration is 1wt%.
Embodiment 7
With embodiment 6, it is differed only in the tobacco aphid method for breeding for being suitable to aphidius gifuensis breeding described in the present embodiment,
The growth promotes liquid also to include the glutamine solution that mass concentration is 2wt%.
Comparative example 1
With embodiment 4, it is differed only in the tobacco aphid method for breeding for being suitable to aphidius gifuensis breeding described in this comparative example,
In the step (3), the photoperiod is L7：D17.
Comparative example 2
With embodiment 4, it is differed only in the tobacco aphid method for breeding for being suitable to aphidius gifuensis breeding described in this comparative example,
In the step (3), it is 5500-7000lx to control intensity of illumination.
Comparative example 3
With embodiment 4, it is differed only in the tobacco aphid method for breeding for being suitable to aphidius gifuensis breeding described in this comparative example,
In the step (3), it is 1200-1800lx to control intensity of illumination.
Experimental example
In a diameter of 9 ㎝ culture dish, one layer of upper berth filter paper, fresh tobacco leaves take 3-4cm2Size is laid in filter paper
On, vacuum side of blade is upward, is changed 1 time per 3d, and injection proper amount of clear water ensures that blade is fresh in ware.1 primiparity larvae aphid is connect per ware,
Observed 1 time per 24h, survival condition is recorded, until larvae aphid grows to adult aphid.During adult aphid, record adult aphid production aphid quantity and survival
Situation, while the larvae aphid produced is removed.Each processing examination aphid quantity is 70.To above-described embodiment 1-8 and comparative example
Larvae aphid survival rate, adult aphid life-span and fecundity the results are shown in Table 1 in 1-3.
Influence of the photoperiod of table 1 to corn leaf aphids adult aphid life-span and fecundity
Above-mentioned result of the test shows, in the method for the invention, photoperiod L10-16 is controlled in aphid growth course：D8-
When 14, intensity of illumination 3000-5000lx, larvae aphid survival rate is high, adult aphid life-span and breeding period length, average reproductive capacity are high；Especially,
Promotion liquid is grown to promote the growth of aphid breeding to set in workshop, and the anniversary for being advantageous to aphid breeds.
Application examples
Method according to above-described embodiment 1-7 carries out cigarette aphid and bred, and takes after inoculation growth 4-5 days and breeding has a large amount of aphids
The tobacco seedlings of worm are moved into inoculation workshop, are put 800 aphidius gifuensis and are maintained at 20-25 DEG C into honeybee, control workshop temperature, relative humidity
It is maintained at 50-80%；Collection is advance in vitro from the larger individual aphidius gifuensis deadlock aphid of aphidius gifuensis honeybee kind preserving chamber
Sprout wings, mass mating 24 hours is carried out after rejecting hyperparasite honeybee；The aphidius gifuensis of mass mating is let fly away and connect in honeybee room, is appointed
It finds cigarette aphid parasitism naturally；After connecing honeybee 2-3 days, aphidius gifuensis to the parasitic rate of cigarette aphid up to more than 95%, in 20-25 DEG C of bar
A large amount of stiff aphids can be formed by 8-10 days under part, stiff aphid 5 days or so develops into aphidius gifuensis into honeybee.
Obviously, above-described embodiment is only intended to clearly illustrate example, and is not the restriction to embodiment.It is right
For those of ordinary skill in the art, can also make on the basis of the above description it is other it is various forms of change or
Change.There is no necessity and possibility to exhaust all the enbodiments.And the obvious change thus extended out or
Among changing still in the protection domain of the invention.
Claims (10)
1. a kind of aphid method for breeding suitable for aphidius gifuensis breeding, it is characterised in that comprise the following steps：
(1) tobacco seedlings are planted：Tobacco seedlings are conventionally planted in glasshouse, after going out true leaf to tobacco seedling growth, by the cigarette
Seedling moves into climatic chamber, standby；
(2) aphid is connect：Tobacco leaf with cigarette aphid is put into the tobacco seedlings of transplanting, completes aphid inoculation；
(3) 24-26 DEG C, humidity 60%-70%, photoperiod L10-16 of weather indoor temperature is controlled：D8-14, intensity of illumination 3000-
5000lx, carry out breeding for aphid.
2. the aphid method for breeding according to claim 1 suitable for aphidius gifuensis breeding, it is characterised in that the step
(1) in, the tobacco seedlings are to grow at least 6 true leaves.
3. the aphid method for breeding according to claim 1 or 2 suitable for aphidius gifuensis breeding, it is characterised in that the step
Suddenly in (2), after the aphid inoculation step, the step of old blade is removed after being also included in one day.
4. the aphid method for breeding for being suitable to aphidius gifuensis breeding according to claim any one of 1-3, it is characterised in that institute
The step of stating in step (3), in addition to aphid be inoculated with again after aphid grows 8-12 days.
5. the aphid method for breeding for being suitable to aphidius gifuensis breeding according to claim any one of 1-4, it is characterised in that institute
State in step (3), be additionally included in climatic chamber and place the step of growth promotes liquid.
6. the aphid method for breeding according to claim 5 suitable for aphidius gifuensis breeding, it is characterised in that the step
(3) in, it is the maltitol solution that mass concentration is 5-10wt% that the growth, which promotes liquid,.
7. the aphid method for breeding according to claim 6 suitable for aphidius gifuensis breeding, it is characterised in that the growth promotees
Feed liquor also includes the glutamine that mass concentration is 1-2wt%.
8. the aphid method for breeding for being suitable to aphidius gifuensis breeding described in claim any one of 1-7 is artificial in the aphidius gifuensis anniversary
The application of breeding field.
9. the anniversary artificial fecundation method of a kind of aphidius gifuensis, it is characterised in that including according to described in claim any one of 1-7
Method for breeding carry out cigarette aphid the step of breeding, and the step of inoculation aphidius gifuensis.
10. the anniversary artificial fecundation method of aphidius gifuensis according to claim 9, it is characterised in that the inoculation step
In, the benefit evil of aphidius gifuensis is than being 1：50-60.
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| CN104642267A (en) * | 2015-02-09 | 2015-05-27 | 云南绿叶生防科技有限公司 | Industrial cultivation method for aphidius gifuensis ashmead |
| JP2016146792A (en) * | 2015-02-13 | 2016-08-18 | 株式会社アグリ総研 | Manufacturing method of bunker type biological pesticide |
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|---|---|---|---|---|
| CN1327738A (en) * | 2001-06-22 | 2001-12-26 | 陈太林 | Composite plant growth promoter able to prevent and eliminate diseases and pests |
| KR20140018480A (en) * | 2012-07-31 | 2014-02-13 | 농업회사법인주식회사 오상킨섹트 | System for observating growth of parasitic insect |
| CN103478083A (en) * | 2013-09-29 | 2014-01-01 | 任伟 | Large-scale breeding method for breeding mummified aphids and adult bees of aphidiusgifuensis by yellow-green myzuspersicae |
| CN104642267A (en) * | 2015-02-09 | 2015-05-27 | 云南绿叶生防科技有限公司 | Industrial cultivation method for aphidius gifuensis ashmead |
| JP2016146792A (en) * | 2015-02-13 | 2016-08-18 | 株式会社アグリ総研 | Manufacturing method of bunker type biological pesticide |
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