CN103385220A - Scale breeding method for encarsia formosa - Google Patents
Scale breeding method for encarsia formosa Download PDFInfo
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- CN103385220A CN103385220A CN2013103135792A CN201310313579A CN103385220A CN 103385220 A CN103385220 A CN 103385220A CN 2013103135792 A CN2013103135792 A CN 2013103135792A CN 201310313579 A CN201310313579 A CN 201310313579A CN 103385220 A CN103385220 A CN 103385220A
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- aleyrodid
- host plant
- honeybee
- encarsia formosa
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- 241001454772 Encarsia formosa Species 0.000 title claims abstract description 107
- 238000009395 breeding Methods 0.000 title claims abstract description 45
- 241000382353 Pupa Species 0.000 claims abstract description 64
- 230000001488 breeding effect Effects 0.000 claims abstract description 39
- 241000819999 Nymphes Species 0.000 claims abstract description 34
- 241000258937 Hemiptera Species 0.000 claims abstract description 22
- 238000000034 method Methods 0.000 claims abstract description 19
- 241000254127 Bemisia tabaci Species 0.000 claims abstract description 9
- 241000018137 Trialeurodes vaporariorum Species 0.000 claims abstract description 5
- 241000256844 Apis mellifera Species 0.000 claims description 132
- 241000196324 Embryophyta Species 0.000 claims description 129
- 102000002322 Egg Proteins Human genes 0.000 claims description 24
- 108010000912 Egg Proteins Proteins 0.000 claims description 24
- 210000004681 ovum Anatomy 0.000 claims description 24
- 238000011081 inoculation Methods 0.000 claims description 12
- 241000238631 Hexapoda Species 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 9
- 235000013601 eggs Nutrition 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 4
- 208000017667 Chronic Disease Diseases 0.000 claims description 4
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims description 4
- 239000006013 carbendazim Substances 0.000 claims description 4
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 4
- 201000010099 disease Diseases 0.000 claims description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 4
- 210000003608 fece Anatomy 0.000 claims description 4
- 239000003337 fertilizer Substances 0.000 claims description 4
- 239000010871 livestock manure Substances 0.000 claims description 4
- 230000013011 mating Effects 0.000 claims description 4
- 230000024241 parasitism Effects 0.000 claims description 4
- 238000004321 preservation Methods 0.000 claims description 4
- 230000002787 reinforcement Effects 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 claims description 4
- 239000013589 supplement Substances 0.000 claims description 4
- 244000017020 Ipomoea batatas Species 0.000 claims description 3
- 235000002678 Ipomoea batatas Nutrition 0.000 claims description 3
- 238000012856 packing Methods 0.000 claims description 3
- 208000035240 Disease Resistance Diseases 0.000 claims description 2
- 244000061458 Solanum melongena Species 0.000 claims description 2
- 235000002597 Solanum melongena Nutrition 0.000 claims description 2
- 230000009286 beneficial effect Effects 0.000 claims description 2
- 230000000903 blocking effect Effects 0.000 claims description 2
- 244000061176 Nicotiana tabacum Species 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 238000005265 energy consumption Methods 0.000 abstract 1
- 241000894007 species Species 0.000 abstract 1
- 238000007726 management method Methods 0.000 description 18
- 238000005516 engineering process Methods 0.000 description 4
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- 206010059866 Drug resistance Diseases 0.000 description 1
- 206010017533 Fungal infection Diseases 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
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- 235000009508 confectionery Nutrition 0.000 description 1
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Abstract
The invention discloses a scale breeding method for encarsia formosa. The scale breeding method is finished in four greenhouses, namely a host plant cultivating room, a worm species room, an encarsia formosa species room and a breeding room, by steps. The scale breeding method comprises a host plant cultivating and managing step, a whitefly species managing step, a whitefly inoculating step, a whitefly nymph breeding step, an encarsia formosa species managing step, an encarsia formosa inoculating step, an encarsia formosa pupa breeding step, a pupa collecting step, a worm card or encarsia formosa box manufacturing and storing step and a whitefly controlling step. The scale breeding method has the advantages that process steps are continuously circulated and the encarsia formosa can be annually produced and bred. The scale breeding method is suitable for industrial scale production and reproduction of the encarsia formosa and is short in reproduction period, high in efficiency, low in energy consumption and low in cost. The obtained adult encarsia formosa, encarsia formosa pupa card or encarsia formosa box products are good in quality. The problem of insufficient supply for control over bemisia tabaci and trialeurodes vaporariorum in agriculture and forestry is solved.
Description
Technical field
The invention belongs to the biological prevention field of agriculture and forestry insect, be specifically related to the scale propagation method of the Encarsia formosa of using in the process of biological control agriculture and forestry injurious insect.
Background technology
aleyrodid is a kind of important pests of farm-forestry crop, all there is aleyrodid endangering to some extent the prodution and development of farm-forestry crop all over the world, aleyrodid is rasping-sucking mouthparts, adult and nymph suck water, the a large amount of sweet liquid of secretion in the harm process, bring out and propagate multiple mycosis, host range is extensive, the harm flowers, fruit tree, medicinal material, 600 various plants of more than 100 section such as tobacco, the aleyrodid population quantity is huge, fertility is strong, reproduction speed is fast, clustering is caused harm, killed plant leaf chlorisis, flavescence, wilt, blade and fruit are subject to polluting the rear quality that affects, cause plant withered when serious.
For a long time, people mainly prevent and treat whitefly hazard by the method that sprays chemical pesticide, make the drug resistance of aleyrodid and pesticide resistance in continuous enhancing, the usage amount of chemical pesticide increases year by year, contaminated environment, threaten to the quality safety of agriculture and forestry product, affects the healthy of people.
Encarsia formosa is the natural enemy of aleyrodid, and Encarsia formosa produces its worm's ovum in the worm's ovum of aleyrodid, the nutrition that its larva sucks the aleyrodid worm's ovum while growing up, thus aleyrodid is eliminated.It is an important subject of at present the aleyrodid insect being carried out biological control that the application Encarsia formosa is eliminated aleyrodid.At present, the level of this research only develops into laboratory stage, and Encarsia formosa survival rate under the artificial propagation condition is low, and the breeding cycle is long, can't realize large batch of annual supply, can not meet the green actual needs of producing of Modern Agriculture forestry.
Summary of the invention
The present invention is directed in prior art, Encarsia formosa survival rate under the artificial propagation condition is low, breeding cycle is long, can not realize large batch of annual supply, can not meet the problem of the green need of production of Modern Agriculture forestry, a kind of scale propagation method of Encarsia formosa is provided, and the method is in host plant raising chamber, worm kind chamber, honeybee kind chamber and breed in the booth of four of chambers substep and complete, and concrete steps are as follows:
(1) host plant is cultivated management
The host plant kind of select is grown seedlings, and in host plant raising chamber carries out potted plantly by batch being transplanted to the seedling that is bred as, cultivates 30-40 days, and is standby after host plant grows 9～12 true leaves; A collection of every transplanting in 15 days when transplanting, guarantee all to have throughout the year the host plant of 9-12 sheet true leaf standby;
A little less than the kind of described host plant required the strong resistance to insects of disease resistance, blade was many, large, and blade face is smooth, was beneficial to aleyrodid breeding and worm blocking work, can be a kind of in flue-cured tobacco, eggplant, sweet potato;
(2) management of aleyrodid worm kind
Collection is put into worm kind chamber without sick body large greenhouse whitefly or Bemisia tabaci adults, put into the standby host plant of (1) step and raise aleyrodid in worm kind chamber, the quantity of putting in a suitable place to breed of aleyrodid is controlled at the potted plant host plant of every strain and puts 30～50 of adult whiteflies in a suitable place to breed, controlling worm kind indoor conditions is 17～27 ℃ of temperature, RH.40～70%, raise after 20 days, the density of worm kind indoor greenhouse aleyrodid or Bemisia tabaci reaches every strain host plant 300～500, obtains aleyrodid worm kind;
Every 15-25 days, host plant in worm kind chamber is carried out the old and new replaces, old host plant with 20%～50% shifts out worm kind chamber, then fetch the new host plant that 9-12 sheet true leaf is arranged from the cultivation canopy and put into worm kind chamber, guarantee the annual host plant raising aleyrodid worm kind that 9-12 sheet true leaf is all arranged in worm kind chamber;
(3) aleyrodid inoculation
Be placed in worm kind chamber with the host plant of 9-12 sheet true leaf, aleyrodid lays eggs at the blade back of this host plant, putting 4-5 days in worm kind chamber after, host plant completes the aleyrodid inoculation, after host plant inoculation aleyrodid worm's ovum, the host plant that has inoculated the aleyrodid worm's ovum is put into to breed in chamber breeds the aleyrodid nymph;
Perhaps utilize portable cocoon honeybee receipts to catch device in worm kind chamber and collect the adult of aleyrodid, then the adult of aleyrodid is discharged into and breeds in chamber, adult whitefly discharges in breeding chamber density is every strain host plant 40-70 head, and adult whitefly is bred the aleyrodid nymph breeding on indoor host plant to lay eggs;
(4) the aleyrodid nymph breeds
Keeping breeding indoor temperature is 17-27 ℃, RH.40-70%, reinforcement is carried out suitable water and fertilizer management to host plant, breed and sprayed 1000 times of liquid sterilizing disease-preventings of carbendazim of one time 25% in 5-8 days to host plant, continue to breed 10-15 days, the quantity of the vacuum side of blade aleyrodid worm's ovum of breeding indoor host plant is bred reach 10～40 every square centimeter, and have the aleyrodid worm's ovum more than 50% to bud into aleyrodid 2-3 nymph in age;
(5) Encarsia formosa honeybee kind management
Collection is without the black pupa of the large Encarsia formosa of sick body, be placed in the cultured insect bottle, it is sprouted wings in the cultivation bottle after, mating is 12 hours, the Encarsia formosa of emergence post-coitum is discharged into the honeybee kind indoor, contain the host plant of 5～20 2-3 aleyrodid in age nymphs on every square centimeter of blade cultivating in honeybee kind indoor placement (4) step, keep 17-27 ℃ of honeybee kind indoor temperature, RH.40-70%, after 14 days in honeybee kind chamber the density of the black pupa of Encarsia formosa reach every strain host plant more than 2000, obtain the black pupa of honeybee kind of Encarsia formosa;
In Encarsia formosa honeybee kind cultivating process, shift out aperiodically chronic illness or gone out the host plant of honeybee, then supplement the host plant that contains 5～20 2-3 aleyrodid in age nymphs of cultivating in (4) step of equal number, guarantee all to have throughout the year the honeybee kind of Encarsia formosa;
(6) Encarsia formosa connects honeybee
In honeybee kind chamber, the black pupa of the honeybee kind of gather to be about to sprouting wings, and the aleyrodid worm's ovum that is discharged into the vacuum side of blade of breeding host plant in chamber has more than 50% on the host plant blade that becomes aleyrodid nymph in 2～3 age;
Perhaps utilize portable cocoon honeybee to receive in honeybee kind chamber and catch the one-tenth honeybee of device collection Encarsia formosa, the one-tenth honeybee that receipts are caught the Encarsia formosa that obtains is discharged into step (4) breeding in chamber after finishing, Encarsia formosa becomes the burst size of honeybee and the quantity ratio of aleyrodid nymph in 2～3 age to be controlled at 1:20～40, and Encarsia formosa is completed and connect honeybee in breeding chamber;
(7) the Encarsia formosa bee pupa is bred
Encarsia formosa connects honeybee and keeps breeding temperature in chamber at 17～27 ℃, RH.40～70% later, the suitable management of host plant liquid manure, cultivated 14～18 days, Encarsia formosa is bred in a large number, aleyrodid nymph 70%～85% on host plant becomes the black pupa of Encarsia formosa, guarantees to breed in large quantity throughout the year the black pupa that obtains Encarsia formosa or becomes honeybee;
(8) bee pupa Collection and preservation
After breeding aleyrodid nymph in chamber and becoming the black pupa of Encarsia formosa, the blade that the aleyrodid nymph has been become the host plant of black pupa more than 85% collects, remove aleyrodid remaining on blade, obtain the bee pupa blade, the bee pupa blade was placed on Indoor Natural air-dry 2～3 days, remove not by the aleyrodid worm's ovum of Encarsia formosa parasitism, air-dry bee pupa blade is directly made the adult card;
Perhaps the bee pupa on air-dry bee pupa blade is separated, be made into the honeybee box in the ventilating box of packing into;
Worm card or honeybee box are stored in 8～10 ℃ of temperature, in the refrigerating chamber of humidity 40～50%;
(9) aleyrodid control
Worm card or honeybee box are taken out from refrigerating chamber, render to the agricultural area control aleyrodid of whitefly hazard before bee pupa is sprouted wings;
Perhaps utilize portable cocoon honeybee receipts to catch device and collect the one-tenth honeybee of Encarsia formosa from breed chamber, will become honeybee to be discharged into the agricultural area control aleyrodid of whitefly hazard.
In processing step of the present invention, in the controlled canopy chamber of environmental condition, adopt host plant to cultivate management and the Encarsia formosa honeybee kind management method of management, aleyrodid worm kind, guarantee all to have in 1 year season host plant to raise the provenance of Encarsia formosa, guarantee that 1 year season all had the honeybee kind to provide; In the present invention, the inoculation of the inoculation of aleyrodid and raising technology, Encarsia formosa and raising technology, can guarantee the whole year of Encarsia formosa, the in enormous quantities supply, and Encarsia formosa can be produced and breed to these technology successively, circularly, the large-scale industrialized Reproduction that adapts to Encarsia formosa, its breeding cycle is short, aquaculture cost is low, the Encarsia formosa that obtains becomes honeybee and bee pupa worm card or honeybee box product quality superior, having solved the Encarsia formosa kind honeybee that prevents and treats Bemisia tabaci and greenhouse whitefly in the agricultural production can't be in batches, continuous supplying to problem.Realized this biological prevention is applied from the laboratory to the land for growing field crops industrialized development target.
Embodiment
Embodiment 1
The scale propagation method of Encarsia formosa, the method is in host plant raising chamber, worm kind chamber, honeybee kind chamber and breed in the booth of four of chambers substep and complete, and concrete steps are as follows:
(1) host plant is cultivated management
Select flue-cured tobacco and grow seedlings as the host plant kind, in host plant raising chamber carry out potted plantly by batch being transplanted to the seedling that is bred as, cultivated 30 days, standby after host plant grows 9～12 true leaves; A collection of every transplanting in 15 days when transplanting, guarantee all to have throughout the year the host plant of 9-12 sheet true leaf standby;
(2) management of aleyrodid worm kind
Collection is put into worm kind chamber without the large Bemisia tabaci adults of sick body, put into the standby host plant of (1) step and raise aleyrodid in worm kind chamber, the quantity of putting in a suitable place to breed of aleyrodid is controlled at the potted plant host plant of every strain and puts 30 of adult whiteflies in a suitable place to breed, controlling worm kind indoor conditions is 17 ℃ of temperature, RH.40%, raise after 20 days, the density of worm kind indoor greenhouse aleyrodid or Bemisia tabaci reaches every strain host plant 300～500, obtains aleyrodid worm kind;
Every 15 days, host plant in worm kind chamber is carried out the old and new replaces, old host plant with 20% shifts out worm kind chamber, then fetch the new host plant that 9-12 sheet true leaf is arranged from the cultivation canopy and put into worm kind chamber, guarantee the annual host plant raising aleyrodid worm kind that 9-12 sheet true leaf is all arranged in worm kind chamber;
(3) aleyrodid inoculation
Be placed in worm kind chamber with the host plant of 9-12 sheet true leaf, aleyrodid lays eggs at the blade back of this host plant, putting 4 days in worm kind chamber after, host plant completes the aleyrodid inoculation, after host plant inoculation aleyrodid worm's ovum, the host plant that has inoculated the aleyrodid worm's ovum is put into to breed in chamber breeds the aleyrodid nymph;
(4) the aleyrodid nymph breeds
Keeping breeding indoor temperature is 17 ℃, RH.40%, reinforcement is carried out suitable water and fertilizer management to host plant, breed and sprayed 1000 times of liquid sterilizing disease-preventings of carbendazim of one time 25% in 5 days to host plant, continued to breed 10 days, the quantity of the vacuum side of blade aleyrodid worm's ovum of breeding indoor host plant is bred reach 10 every square centimeter, and have the aleyrodid worm's ovum more than 50% to bud into aleyrodid 2-3 nymph in age;
(5) Encarsia formosa honeybee kind management
Collection is without the black pupa of the large Encarsia formosa of sick body, be placed in the cultured insect bottle, it is sprouted wings in the cultivation bottle after, mating is 12 hours, the Encarsia formosa of emergence post-coitum is discharged into the honeybee kind indoor, contain the host plant of 5 2-3 aleyrodid in age nymphs on every square centimeter of blade cultivating in honeybee kind indoor placement (4) step, keep 17 ℃ of honeybee kind indoor temperatures, RH.40%, after 14 days in honeybee kind chamber the density of the black pupa of Encarsia formosa reach every strain host plant more than 2000, obtain the black pupa of honeybee kind of Encarsia formosa;
In Encarsia formosa honeybee kind cultivating process, shift out aperiodically chronic illness or gone out the host plant of honeybee, then supplement the host plant that contains 5 2-3 aleyrodid in age nymphs of cultivating in (4) step of equal number, guarantee all to have throughout the year the honeybee kind of Encarsia formosa;
(6) Encarsia formosa connects honeybee
In honeybee kind chamber, the black pupa of the honeybee kind of gather to be about to sprouting wings, and the aleyrodid worm's ovum that is discharged into the vacuum side of blade of breeding host plant in chamber has more than 50% on the host plant blade that becomes aleyrodid nymph in 2～3 age;
(7) the Encarsia formosa bee pupa is bred
Encarsia formosa connects honeybee and keeps breeding temperature in chamber at 17 ℃, RH.40% later, the suitable management of host plant liquid manure, cultivated 18 days, Encarsia formosa is bred in a large number, aleyrodid nymph 70% on host plant becomes the black pupa of Encarsia formosa, guarantees to breed in large quantity throughout the year the black pupa that obtains Encarsia formosa or becomes honeybee;
(8) bee pupa Collection and preservation
After breeding aleyrodid nymph in chamber and becoming the black pupa of Encarsia formosa, the blade that the aleyrodid nymph has been become the host plant of black pupa more than 85% collects, remove aleyrodid remaining on blade, obtain the bee pupa blade, the bee pupa blade was placed on Indoor Natural air-dry 2 days, remove not by the aleyrodid worm's ovum of Encarsia formosa parasitism, air-dry bee pupa blade is directly made the adult card;
The worm card is stored in 8 ℃ of temperature, in the refrigerating chamber of humidity 40%;
(9) aleyrodid control
The worm card is taken out from refrigerating chamber, render to the agricultural area control aleyrodid of whitefly hazard before bee pupa is sprouted wings.
Embodiment 2
The scale propagation method of Encarsia formosa, the method is in host plant raising chamber, worm kind chamber, honeybee kind chamber and breed in the booth of four of chambers substep and complete, and concrete steps are as follows:
(1) host plant is cultivated management
Select sweet potato and grow seedlings as the host plant kind, in cultivation canopy carry out potted plantly by batch being transplanted to the seedling that is bred as, cultivated 40 days, standby after host plant grows 9～12 true leaves; A collection of every transplanting in 15 days when transplanting, guarantee all to have throughout the year the host plant of 9-12 sheet true leaf standby;
(2) management of aleyrodid worm kind
Collection is put into worm kind chamber without the large greenhouse whitefly adult of sick body, put into the standby host plant of (1) step and raise aleyrodid in worm kind chamber, the quantity of putting in a suitable place to breed of aleyrodid is controlled at the potted plant host plant of every strain and puts 50 of adult whiteflies in a suitable place to breed, controlling worm kind indoor conditions is 27 ℃ of temperature, RH. 70%, raise after 20 days, the density of worm kind indoor greenhouse aleyrodid or Bemisia tabaci reaches every strain host plant 500, obtains aleyrodid worm kind;
Every 25 days, host plant in worm kind chamber is carried out the old and new replaces, old host plant with 50% shifts out worm kind chamber, then fetch the new host plant that 9-12 sheet true leaf is arranged from the cultivation canopy and put into worm kind chamber, guarantee the annual host plant raising aleyrodid worm kind that 9-12 sheet true leaf is all arranged in worm kind chamber;
(3) aleyrodid inoculation
Utilize portable cocoon honeybee receipts to catch device in worm kind chamber and collect the adult of aleyrodid, then the adult of aleyrodid is discharged into and breeds in chamber, adult whitefly discharges in breeding chamber density is 40 of every strain host plants, and adult whitefly is bred the aleyrodid nymph breeding on indoor host plant to lay eggs;
(4) the aleyrodid nymph breeds
Keeping breeding indoor temperature is 27 ℃, RH.70%, reinforcement is carried out suitable water and fertilizer management to host plant, breed and sprayed 1000 times of liquid sterilizing disease-preventings of carbendazim of one time 25% in 8 days to host plant, continued to breed 15 days, the quantity of the vacuum side of blade aleyrodid worm's ovum of breeding indoor host plant is bred reach 40 every square centimeter, and have the aleyrodid worm's ovum more than 50% to bud into aleyrodid 2-3 nymph in age;
(5) Encarsia formosa honeybee kind management
Collection is without the black pupa of the large Encarsia formosa of sick body, be placed in the cultured insect bottle, it is sprouted wings in the cultivation bottle after, mating is 12 hours, the Encarsia formosa of emergence post-coitum is discharged into the honeybee kind indoor, contain the host plant of 20 2-3 aleyrodid in age nymphs on every square centimeter of blade cultivating in honeybee kind indoor placement (4) step, keep 27 ℃ of honeybee kind indoor temperatures, RH.70%, after 14 days in honeybee kind chamber the density of the black pupa of Encarsia formosa reach every strain host plant more than 2000, obtain the black pupa of honeybee kind of Encarsia formosa;
In Encarsia formosa honeybee kind cultivating process, shift out aperiodically chronic illness or gone out the host plant of honeybee, then supplement the host plant that contains 20 2-3 aleyrodid in age nymphs of cultivating in (4) step of equal number, guarantee all to have throughout the year the honeybee kind of Encarsia formosa;
(6) Encarsia formosa connects honeybee
Utilize portable cocoon honeybee to receive in honeybee kind chamber and catch the one-tenth honeybee of device collection Encarsia formosa, the one-tenth honeybee that receipts are caught the Encarsia formosa that obtains is discharged into step (4) breeding in chamber after finishing, Encarsia formosa becomes the burst size of honeybee and the quantity ratio of aleyrodid nymph in 2～3 age to be controlled at 1:20, and Encarsia formosa is completed and connect honeybee in breeding chamber;
(7) the Encarsia formosa bee pupa is bred
Encarsia formosa connects honeybee and keeps breeding temperature in chamber at 27 ℃, RH. 70% later, the suitable management of host plant liquid manure, cultivated 14 days, Encarsia formosa is bred in a large number, aleyrodid nymph 85% on host plant becomes the black pupa of Encarsia formosa, guarantees to breed in large quantity throughout the year the black pupa that obtains Encarsia formosa or becomes honeybee;
(8) bee pupa Collection and preservation
After breeding aleyrodid nymph in chamber and becoming the black pupa of Encarsia formosa, the blade that the aleyrodid nymph has been become the host plant of black pupa more than 85% collects, remove aleyrodid remaining on blade, obtain the bee pupa blade, the bee pupa blade was placed on Indoor Natural air-dry 3 days, remove not by the aleyrodid worm's ovum of Encarsia formosa parasitism, the bee pupa on air-dry bee pupa blade is separated, be made into the honeybee box in the ventilating box of packing into;
The honeybee box is stored in 10 ℃ of temperature, in the refrigerating chamber of humidity 50%;
(9) aleyrodid control
The honeybee box is taken out from refrigerating chamber, render to the agricultural area control aleyrodid of whitefly hazard before bee pupa is sprouted wings;
Utilize portable cocoon honeybee receipts to catch device and collect the one-tenth honeybee of Encarsia formosa from breed chamber, will become honeybee to be discharged into the agricultural area control aleyrodid of whitefly hazard.
Claims (2)
1. the scale propagation method of Encarsia formosa is characterized in that: the method is in host plant raising chamber, worm kind chamber, honeybee kind chamber and breed substep in the booth of four of chambers and complete, and concrete steps are as follows:
(1) host plant is cultivated management
The host plant kind of select is grown seedlings, and in host plant raising chamber carries out potted plantly by batch being transplanted to the seedling that is bred as, cultivates 30-40 days, and is standby after host plant grows 9～12 true leaves; A collection of every transplanting in 15 days when transplanting, guarantee all to have throughout the year the host plant of 9-12 sheet true leaf standby;
(2) management of aleyrodid worm kind
Collection is put into worm kind chamber without sick body large greenhouse whitefly or Bemisia tabaci adults, put into the standby host plant of (1) step and raise aleyrodid in worm kind chamber, the quantity of putting in a suitable place to breed of aleyrodid is controlled at the potted plant host plant of every strain and puts 30～50 of adult whiteflies in a suitable place to breed, controlling worm kind indoor conditions is 17～27 ℃ of temperature, RH.40～70%, raise after 20 days, the density of worm kind indoor greenhouse aleyrodid or Bemisia tabaci reaches every strain host plant 300～500, obtains aleyrodid worm kind;
Every 15-25 days, host plant in worm kind chamber is carried out the old and new replaces, old host plant with 20%～50% shifts out worm kind chamber, then fetch the new host plant that 9-12 sheet true leaf is arranged from the cultivation canopy and put into worm kind chamber, guarantee the annual host plant raising aleyrodid worm kind that 9-12 sheet true leaf is all arranged in worm kind chamber;
(3) aleyrodid inoculation
Be placed in worm kind chamber with the host plant of 9-12 sheet true leaf, aleyrodid lays eggs at the blade back of this host plant, putting 4-5 days in worm kind chamber after, host plant completes the aleyrodid inoculation, after host plant inoculation aleyrodid worm's ovum, the host plant that has inoculated the aleyrodid worm's ovum is put into to breed in chamber breeds the aleyrodid nymph;
Perhaps utilize portable cocoon honeybee receipts to catch device in worm kind chamber and collect the adult of aleyrodid, then the adult of aleyrodid is discharged into and breeds in chamber, adult whitefly discharges in breeding chamber density is every strain host plant 40-70 head, and adult whitefly is bred the aleyrodid nymph breeding on indoor host plant to lay eggs;
(4) the aleyrodid nymph breeds
Keeping breeding indoor temperature is 17-27 ℃, RH.40-70%, reinforcement is carried out suitable water and fertilizer management to host plant, breed and sprayed 1000 times of liquid sterilizing disease-preventings of carbendazim of one time 25% in 5-8 days to host plant, continue to breed 10-15 days, the quantity of the vacuum side of blade aleyrodid worm's ovum of breeding indoor host plant is bred reach 10～40 every square centimeter, and have the aleyrodid worm's ovum more than 50% to bud into aleyrodid 2-3 nymph in age;
(5) Encarsia formosa honeybee kind management
Collection is without the black pupa of the large Encarsia formosa of sick body, be placed in the cultured insect bottle, it is sprouted wings in the cultivation bottle after, mating is 12 hours, the Encarsia formosa of emergence post-coitum is discharged into the honeybee kind indoor, contain the host plant of 5～20 2-3 aleyrodid in age nymphs on every square centimeter of blade cultivating in honeybee kind indoor placement (4) step, keep 17-27 ℃ of honeybee kind indoor temperature, RH.40-70%, after 14 days in honeybee kind chamber the density of the black pupa of Encarsia formosa reach every strain host plant more than 2000, obtain the black pupa of honeybee kind of Encarsia formosa;
In Encarsia formosa honeybee kind cultivating process, shift out aperiodically chronic illness or gone out the host plant of honeybee, then supplement the host plant that contains 5～20 2-3 aleyrodid in age nymphs of cultivating in (4) step of equal number, guarantee all to have throughout the year the honeybee kind of Encarsia formosa;
(6) Encarsia formosa connects honeybee
In honeybee kind chamber, the black pupa of the honeybee kind of gather to be about to sprouting wings, and the aleyrodid worm's ovum that is discharged into the vacuum side of blade of breeding host plant in chamber has more than 50% on the host plant blade that becomes aleyrodid nymph in 2～3 age;
Perhaps utilize portable cocoon honeybee to receive in honeybee kind chamber and catch the one-tenth honeybee of device collection Encarsia formosa, the one-tenth honeybee that receipts are caught the Encarsia formosa that obtains is discharged into step (4) breeding in chamber after finishing, Encarsia formosa becomes the burst size of honeybee and the quantity ratio of aleyrodid nymph in 2～3 age to be controlled at 1:20～40, and Encarsia formosa is completed and connect honeybee in breeding chamber;
(7) the Encarsia formosa bee pupa is bred
Encarsia formosa connects honeybee and keeps breeding temperature in chamber at 17～27 ℃, RH.40～70% later, the suitable management of host plant liquid manure, cultivated 14～18 days, Encarsia formosa is bred in a large number, aleyrodid nymph 70%～85% on host plant becomes the black pupa of Encarsia formosa, guarantees to breed in large quantity throughout the year the black pupa that obtains Encarsia formosa or becomes honeybee;
(8) bee pupa Collection and preservation
After breeding aleyrodid nymph in chamber and becoming the black pupa of Encarsia formosa, the blade that the aleyrodid nymph has been become the host plant of black pupa more than 85% collects, remove aleyrodid remaining on blade, obtain the bee pupa blade, the bee pupa blade was placed on Indoor Natural air-dry 2～3 days, remove not by the aleyrodid worm's ovum of Encarsia formosa parasitism, air-dry bee pupa blade is directly made the adult card;
Perhaps the bee pupa on air-dry bee pupa blade is separated, be made into the honeybee box in the ventilating box of packing into;
Worm card or honeybee box are stored in 8～10 ℃ of temperature, in the refrigerating chamber of humidity 40～50%;
(9) aleyrodid control
Worm card or honeybee box are taken out from refrigerating chamber, render to the agricultural area control aleyrodid of whitefly hazard before bee pupa is sprouted wings;
Perhaps utilize portable cocoon honeybee receipts to catch device and collect the one-tenth honeybee of Encarsia formosa from breed chamber, will become honeybee to be discharged into the agricultural area control aleyrodid of whitefly hazard.
2. the scale propagation method of Encarsia formosa as claimed in claim 1, it is characterized in that: a little less than the kind of described host plant required the strong resistance to insects of disease resistance, blade was many, large, and blade face is smooth, being beneficial to aleyrodid breeding and worm blocking work, can be a kind of in flue-cured tobacco, eggplant, sweet potato.
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Effective date of registration: 20170407 Address after: 653100 Yunnan City, Yuxi province Hongta District Dongfeng Road, No. 3, building 24, 21 Patentee after: Yunnan greenery biological and ecological methods to prevent plant disease, pests, and erosion Science and Technology Ltd. Address before: Yuxi City, Yunnan Province, 653100 scientific research base Patentee before: Ren Wei |