CN104438293A - Microbial detoxification method of cottonseed cakes - Google Patents
Microbial detoxification method of cottonseed cakes Download PDFInfo
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- CN104438293A CN104438293A CN201410708305.8A CN201410708305A CN104438293A CN 104438293 A CN104438293 A CN 104438293A CN 201410708305 A CN201410708305 A CN 201410708305A CN 104438293 A CN104438293 A CN 104438293A
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- cake dregs
- cotton cake
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- fermentation
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Abstract
The invention discloses a microbial detoxification method of cottonseed cakes. A cottonseed cake solid fermentation medium is inoculated with an Aspergillus niger and saccharomyces cerevisiae mixed bacterium liquid for solid fermentation, the fermentation temperature is 30 DEG C, the inoculum size V/W is 15%, the initial moisture content is 48% in percentage by weight, the initial pH value is 6, and the fermentation time is 60 h. The detoxification rate of free gossypol in fermented cottonseed cakes is 95.51%, the true protein content is increased from 32.99% to 42.40%, lysine, methionine and threonine in essential amino acids are increased by 31.03%, 25.00% and 44.23% respectively compared with those before fermentation, and the NSI (nitrogen soluble index) is increased by 1.49 times, so that the quality of the cottonseed cakes is improved.
Description
Technical field
The present invention relates to field of microbial fermentation, relate to a kind of method of microbiological treatment cottonseed cake.
Background technology
China is one of Cotton Production big country in the world, and have about 1,000 ten thousand tons cottonseeds every year, stock number is the first in the world.Cotton cake dregs is one of vegetable protein resource very always, and be the byproduct after extracting cottonseed oil, China produces cotton cake dregs about 6,000,000 tons per year.Can obtain amino acid in 17 after China's cotton cake dregs hydrolysis, wherein the necessary amino acid of Palestine and China and soybean protein are relatively.
Although the nutrition of cotton cake dregs is enriched, the 0.7^%-4.8% accounting for common cottonseed, the gossypol toxin of 0.2-2.8% accounting for cotton cake dregs constrain it and utilize space.
Gossypol is again cotton toxin, and sterling is yellow crystal, can be dissolved in most of organic solvent, and as acetone, ether, chloroform, methyl alcohol etc., easy glycerine hard to tolerate, cyclohexane and benzene, be insoluble to low boiling benzinum and water.Existing poison-removing method cost is high, complex process, is not suitable for enterprise's production application on a small scale.
Summary of the invention
Given this, the object of the invention is to provide that a kind of cost is low, the simple de-poisoning method for cottonseed cake of technique.
For solving above technical problem, technical scheme provided by the invention is, a kind of cotton cake dregs microbiological detoxifying method is provided, use aspergillus niger and saccharomyces cerevisiae mixed bacteria liquid to be inoculated into cotton cake dregs solid-state fermentation culture medium and carry out solid state fermentation: fermentation temperature 30 DEG C, inoculum concentration V/W=15%, original water content is 48% by weight percentage, and initial pH value is 6, fermentation time 60h.
Preferably, in described aspergillus niger and saccharomyces cerevisiae Mixed Microbes, aspergillus niger and saccharomyces cerevisiae bacterium colony number are than being 1:1.
Preferably, described aspergillus niger activates on slant medium, and then accesses seed culture medium and cultivate, as solid state fermentation seed liquor after 48h as 30 DEG C of constant-temperature tables; Described saccharomyces cerevisiae activates on slant medium, and then accesses seed culture medium and cultivate, as solid state fermentation seed liquor after 48h as 30 DEG C of constant-temperature tables.
Preferably, described slant medium components based on weight percentage is respectively: potato 16.1%, glucose 1.6%, agar 1.6% and water 80.7%.
Preferably, described seed culture medium is respectively by weight percentage: potato 16.4%, glucose 1.6% and water 82%.
Preferably, described cotton cake dregs solid-state fermentation culture medium is respectively by weight percentage: Cottonseed Meal 68.38%, corn flour 19.54%, wheat bran 9.77%, glucose 0.98%, (NH
4)
2s0
40.98%, MgS0
4.7H
200.1%, K
2hP0
40.2%, MnS0
40.05%.
Compared with prior art, a technical scheme tool in technique scheme has the following advantages:
After fermentation, cotton cake dregs Free Gossypol virus elimination rate is 95.51%, true protein content brings up to 42.40% from 32.99%, in essential amino acid, lysine, methionine and threonine are compared before comparatively fermenting respectively and are added 31.03%, 25.00% and 44.23%, nitrogen soluble index (NSI) adds 1.49 times, thus improves the quality of cotton dregs grouts.
Detailed description of the invention
Aspergillus niger used herein and saccharomyces cerevisiae are commercial.
Aspergillus niger activates on slant medium, and then accesses seed culture medium and cultivate, as solid state fermentation seed liquor after 48h as 30 DEG C of constant-temperature tables.Saccharomyces cerevisiae activates on slant medium, and then accesses seed culture medium and cultivate, as solid state fermentation seed liquor after 48h as 30 DEG C of constant-temperature tables.Slant medium component is: potato 40g, glucose 4g, agar 4g and water 200g; PH nature, 0.1MPa, 121 DEG C of sterilizing 30min.Seed culture medium is respectively by weight percentage: potato 40g, glucose 4g and water 200g; PH nature, 0.1MPa, 121 DEG C of sterilizing 30min.
Use aspergillus niger and saccharomyces cerevisiae mixed bacteria liquid to be inoculated into cotton cake dregs solid-state fermentation culture medium by 1:1 and carry out solid state fermentation.Fermentation temperature 30 DEG C, inoculum concentration V/W=15%, original water content is 48% by weight percentage, and initial pH value is 6, fermentation time 60h.
Cotton cake dregs solid-state fermentation culture medium is respectively by weight percentage: Cottonseed Meal 56g, corn flour 16g, wheat bran 8g, glucose 0.8g, (NH
4)
2s0
40.8g, MgS0
4.7H
200.4g, K
2hP0
40.04g, MnS0
40.02g.
Before and after the fermentation of seedcake dregs of rice culture medium, leading indicator is compared as follows shown in table
Claims (6)
1. a cotton cake dregs microbiological detoxifying method, it is characterized in that, use aspergillus niger and saccharomyces cerevisiae mixed bacteria liquid to be inoculated into cotton cake dregs solid-state fermentation culture medium and carry out solid state fermentation: fermentation temperature 30 DEG C, inoculum concentration V/W=15%, original water content is 48% by weight percentage, initial pH value is 6, fermentation time 60h.
2. cotton cake dregs microbiological detoxifying method according to claim 1, is characterized in that, in described aspergillus niger and saccharomyces cerevisiae Mixed Microbes, aspergillus niger and saccharomyces cerevisiae bacterium colony number are than being 1:1.
3. cotton cake dregs microbiological detoxifying method according to claim 1 and 2, is characterized in that, described aspergillus niger activates on slant medium, and then accesses seed culture medium and cultivate, as solid state fermentation seed liquor after 48h as 30 DEG C of constant-temperature tables; Described saccharomyces cerevisiae activates on slant medium, and then accesses seed culture medium and cultivate, as solid state fermentation seed liquor after 48h as 30 DEG C of constant-temperature tables.
4. cotton cake dregs microbiological detoxifying method according to claim 3, is characterized in that, described slant medium components based on weight percentage is respectively: potato 16.1%, glucose 1.6%, agar 1.6% and water 80.7%.
5. cotton cake dregs microbiological detoxifying method according to claim 3, is characterized in that, described seed culture medium is respectively by weight percentage: potato 16.4%, glucose 1.6% and water 82%.
6. cotton cake dregs microbiological detoxifying method according to claim 3, is characterized in that, described cotton cake dregs solid-state fermentation culture medium is respectively by weight percentage: Cottonseed Meal 68.38%, corn flour 19.54%, wheat bran 9.77%, glucose 0.98%, (NH
4)
2s0
40.98%, MgS0
4.7H
200.1%, K
2hP0
40.2%, MnS0
40.05%.
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|---|---|---|---|
| CN201410708305.8A CN104438293A (en) | 2014-11-27 | 2014-11-27 | Microbial detoxification method of cottonseed cakes |
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|---|---|---|---|
| CN201410708305.8A CN104438293A (en) | 2014-11-27 | 2014-11-27 | Microbial detoxification method of cottonseed cakes |
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| CN104438293A true CN104438293A (en) | 2015-03-25 |
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| KR20090070631A (en) * | 2007-12-27 | 2009-07-01 | 윤순복 | Fermented feed additives for replacing antibiotics and method of manufacturing thereof |
| CN101773203A (en) * | 2010-03-05 | 2010-07-14 | 中国农业科学院油料作物研究所 | Method for biologically improving oilseed residue |
| CN102318735A (en) * | 2011-09-23 | 2012-01-18 | 济宁市天瑞通科技开发有限公司 | Antibiotic-free mixed feedstuff and preparation method thereof |
| CN102669483A (en) * | 2012-06-09 | 2012-09-19 | 武汉施瑞福生物技术有限公司 | Mixed plant protein fish feed as well as preparation method and application thereof |
| CN102823726A (en) * | 2012-09-07 | 2012-12-19 | 北京大北农科技集团股份有限公司 | Biological fermentation method capable of improving protein content of cottonseed meal and removing toxicity |
| CN103355472A (en) * | 2013-01-21 | 2013-10-23 | 上海创博生态工程有限公司 | Microbial fermentation agent for cottonseed meal detoxification and preparation method and application thereof |
-
2014
- 2014-11-27 CN CN201410708305.8A patent/CN104438293A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20090070631A (en) * | 2007-12-27 | 2009-07-01 | 윤순복 | Fermented feed additives for replacing antibiotics and method of manufacturing thereof |
| CN101773203A (en) * | 2010-03-05 | 2010-07-14 | 中国农业科学院油料作物研究所 | Method for biologically improving oilseed residue |
| CN102318735A (en) * | 2011-09-23 | 2012-01-18 | 济宁市天瑞通科技开发有限公司 | Antibiotic-free mixed feedstuff and preparation method thereof |
| CN102669483A (en) * | 2012-06-09 | 2012-09-19 | 武汉施瑞福生物技术有限公司 | Mixed plant protein fish feed as well as preparation method and application thereof |
| CN102823726A (en) * | 2012-09-07 | 2012-12-19 | 北京大北农科技集团股份有限公司 | Biological fermentation method capable of improving protein content of cottonseed meal and removing toxicity |
| CN103355472A (en) * | 2013-01-21 | 2013-10-23 | 上海创博生态工程有限公司 | Microbial fermentation agent for cottonseed meal detoxification and preparation method and application thereof |
Non-Patent Citations (1)
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|---|
| 吴伟伟: "复合微生物固态发酵棉籽饼粕的研究", 《中国优秀硕士学位论文全文数据库（电子期刊） 农业科技特辑》 * |
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Application publication date: 20150325 |