CN103461654A - Method for producing vinasse protein feed from corn steep liquor - Google Patents
Method for producing vinasse protein feed from corn steep liquor Download PDFInfo
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- CN103461654A CN103461654A CN2013104742415A CN201310474241A CN103461654A CN 103461654 A CN103461654 A CN 103461654A CN 2013104742415 A CN2013104742415 A CN 2013104742415A CN 201310474241 A CN201310474241 A CN 201310474241A CN 103461654 A CN103461654 A CN 103461654A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention discloses a method for producing a vinasse protein feed from corn steep liquor. The method comprises the steps of preparing a culture medium, activating by yeast strains, preparing a fermenting culture medium, fermenting inoculating, inoculating lactic acid bacteria for fermenting again, adsorbing for concentrating, drying, and packaging so as to obtain the finished product. By adoption of the method, a current process of producing ethanol from corns is changed, the vinasse protein feed with the equal effect can be obtained by mainly using corn starch production side products such as corn steep liquor and corn bran through microbial fermentation treatment and together with fully-automatic fermentation culture control, the function of the vinasse protein feed is increased, the waste recycling is realized, and corns are saved. The vinasse protein feed has the advantage of not occupying grains needed by humans, environment pollution is alleviated, the production cost is reduced, and excellent economic benefits are achieved.
Description
technical field
The present invention relates to the renovation technique field of traditional Alcohol Production industry, relate in particular to a kind of method that corn steep liquor is produced the vinasse protein feed.
Background technology
21st century is the epoch of bioscience, according to scholarly forecast, this century agricultural and medical product will have more than 70% and derive from biotechnology, the agricultural biotechnologies research of China still is in the starting stage, but the application agriculturally of a small amount of scientific and technological achievement is arranged, shown good effect and wide market prospects.
Continuous increase along with corn is measured in Alcohol Production, also produced a large amount of accessory substances-maize alcohol stillage protein feed (DDGS) simultaneously.Due to the protein content of DDGS, more than 26%, oneself becomes a kind of novel protein feed raw material of domestic and international Feedstuff Enterprises extensive use, is widely used in the cultivation of livestock and poultry and aquatic products.
This problem enlarges and cultivates on the basis of research selecting yeast bacterium and the many fungus strain fermentations of lactic acid bacteria, the component inoculation, a large amount of accessory substance corn steep liquor, the maize peels that produce in the cornstarch industrial production of take are primary raw material, add the compositions such as accessory substance molasses that appropriate soybean protein produces and compound sugar, fermentation under given conditions, make multi-functional improvement DDGS micro-ecological feed through post processing absorption, concentrated, drying.Three-waste free discharge in production process, have good economic and social benefit.
Summary of the invention
The present invention has changed the technique of in the past using Maize Production alcohol; main production of corn starch accessory substance corn steep liquor, the maize peel of adopting; processing undergoes microbial fermentation; and coordinate full-automatic cultivation of fermentation to control; can obtain the DDGS feed of equivalence with it, and increase the function of DDGS, realize refuse reclamation; save corn, there is the advantage of with the people, not striving grain; Reduced environmental pollution; Reduce production cost, there is good economic benefit.
The present invention adopts following technical scheme:
The method that corn steep liquor of the present invention is produced the vinasse protein feed comprises the following steps:
(1) culture medium preparation, join the raw material of following percentage by weight in culture tank corn steep liquor 25-35%, molasses 25-35%, water 30-50%; and stir; then be warming up to 100 ℃, insulation 30min carries out disinfection, and is cooled to room temperature after having sterilized standby;
(2) barms activation, put into warm water by barms, activated 20min;
(3) by cooling good culture medium in the barms access step (1) activate in step (2), regulate temperature at 28-30 ℃, cultivation 60-72h, reach 1.0 hundred million when above until yeast quantity, stops cultivation, makes seed culture fluid standby;
(4) fermentation medium preparation, join the raw material of following percentage by weight in fermentation tank corn steep liquor 50-60%, molasses 25-30%, water 10-20%; and stir; then be warming up to 100 ℃, insulation 30min carries out disinfection, and is cooled to room temperature after having sterilized standby;
(5) fermentation inoculation, the seed culture fluid that step (3) is made is squeezed in fermentation tank and is cultivated with pump, regulates temperature at 30-35 ℃, cultivates 40-60h;
(6) access lactic acid thalline ferments again, and until fermentation tank in, during sugar consumption 40-50%, the access lactic acid bacteria, control temperature 30-35 ℃, and sugar≤1.5% continues fermentation 50-60h, until sugared, has consumed;
(7) after fermentation ends, put tank, with maize peel, carry out Adsorption Concentration, pack to obtain finished product after drying.
In step (1), the raw material percentage of culture medium consists of: corn steep liquor 30%, molasses 30%, water 40%.
In step (4), the raw material percentage of fermentation medium consists of: corn steep liquor 55%, molasses 30%, water 15%.
In step (5) and step (6), in sweat, pH is strict controlled between 4.5-5.5, will open and stir or pass into air stirring when adjusting pH, to guarantee the uniformity of pH.
In step (7), the moisture of dry rear finished product≤12%.
In step (7), the granularity >=0.5mm of finished product.
One, project implementation scheme, processing technology routine:
It is raw material that corn steep liquor, maize peel are take in the present invention, adds carbon source, on the basis of seed selection excellent species, enlarge cultivate, inoculation in batches, biofermentation under given conditions, Adsorption Concentration, drying are produced finished product.
1, the suitable bacterial classification of screening in the bacterial classifications such as saccharomyces cerevisiae, lactic acid bacteria, selection and improvement, enlarge and cultivate.
2, explore zymotechnique, the improvement feed taste, be lowered into wood, reduces and pollute.
The technique of traditional DDGS is to take corn as primary raw material, and through liquefaction, saccharification, fermentation alcohol, isolate DDGS.The primary raw material of starch industry is also corn.In addition, in producing starch and alcohol process, produce a large amount of waste water and accessory substance.Especially soak water of maize, most producers directly are discharged into sewer.Pollute environment, wasted again resource.
The present invention, according to alcoholic fermentation process, with corn's by-products such as corn steep liquor, maize peels, in conjunction with materials such as carbohydrates, by fermentation, when producing a certain amount of alcohol, stops fermentation.Extract fermentation culture medium, produce DDGS; Can reach equally the DDGS product higher than national standard;
Main feature of the present invention:
One, whole production process does not re-use corn, can save corn.
Two, can solve the emission problem of soak water of maize.
Three, the DDGS produced like this, can also meet on the nutrition foundation of DDGS by changing fermented bacterium, adopts saccharomycete and lactic acid bacteria combined ferment, gives the DDGS feed micro-ecological functions.
Four, fermentation ends, adopt plant cellulose, hemicellulose Adsorption Concentration, there is no discharge of wastewater, reduced the pollution of discharge to environment.
the specific embodiment
The following examples are to describe in further detail of the present invention.
Embodiment 1
(1) culture medium preparation, join the raw material of following percentage by weight in culture tank, corn steep liquor 25%, molasses 25%, water 50%, and stir, then being warming up to 100 ℃, insulation 30min carries out disinfection, and is cooled to room temperature after having sterilized standby;
(2) barms activation, put into warm water by barms, activated 20min;
(3) by cooling good culture medium in the barms access step (1) activate in step (2), regulate temperature at 28 ℃, cultivation 60-72h, reach 1.0 hundred million when above until yeast quantity, stops cultivation, makes seed culture fluid standby;
(4) fermentation medium preparation, join the raw material of following percentage by weight in fermentation tank, corn steep liquor 50%, molasses 25%, water 25%, and stir, then being warming up to 100 ℃, insulation 30min carries out disinfection, and is cooled to room temperature after having sterilized standby;
(5) fermentation inoculation, the seed culture fluid that step (3) is made is squeezed in fermentation tank and is cultivated with pump, regulates temperature at 30 ℃, cultivates 40-60h;
(6) access lactic acid thalline is in fermentation, and until fermentation tank in, during sugar consumption 40%, the access lactic acid bacteria, control 30 ℃ of temperature, and sugar≤1.5% continues fermentation 50-60h, until sugared, has consumed;
(7) after fermentation ends, put tank, with maize peel, carry out Adsorption Concentration, obtain finished product after drying.
In step (5) and step (6), in sweat, pH is strict controlled in 4.5, will open and stir or pass into air stirring when adjusting pH, to guarantee the uniformity of pH.
In step (7), the moisture of dry rear finished product≤12%.
In step (7), the granularity >=0.5mm of finished product.
Embodiment 2
(1) culture medium preparation, join the raw material of following percentage by weight in culture tank, corn steep liquor 35%, molasses 35%, water 30%, and stir, then being warming up to 100 ℃, insulation 30min carries out disinfection, and is cooled to room temperature after having sterilized standby;
(2) barms activation, put into warm water by barms, activated 20min;
(3) by cooling good culture medium in the barms access step (1) activate in step (2), regulate temperature at 30 ℃, cultivation 60-72h, reach 1.0 hundred million when above until yeast quantity, stops cultivation, makes seed culture fluid standby;
(4) fermentation medium preparation, join the raw material of following percentage by weight in fermentation tank, corn steep liquor 60%, molasses 30%, water 10%, and stir, then being warming up to 100 ℃, insulation 30min carries out disinfection, and is cooled to room temperature after having sterilized standby;
(5) fermentation inoculation, the seed culture fluid that step (3) is made is squeezed in fermentation tank and is cultivated with pump, regulates temperature at 35 ℃, cultivates 40-60h;
(6) access lactic acid thalline is in fermentation, and until fermentation tank in, during sugar consumption 50%, the access lactic acid bacteria, control 35 ℃ of temperature, and sugar≤1.5% continues fermentation 50-60h, until sugared, has consumed;
(7) after fermentation ends, put tank, with maize peel, carry out Adsorption Concentration, obtain finished product after drying.
In step (5) and step (6), in sweat, pH is strict controlled in 5.5, will open and stir or pass into air stirring when adjusting pH, to guarantee the uniformity of pH.
In step (7), the moisture of dry rear finished product≤12%.
In step (7), the granularity >=0.5mm of finished product.
Embodiment 3
(1) culture medium preparation, join the raw material of following percentage by weight in culture tank, corn steep liquor 30%, molasses 30%, water 40%, and stir, then being warming up to 100 ℃, insulation 30min carries out disinfection, and is cooled to room temperature after having sterilized standby;
(2) barms activation, put into warm water by barms, activated 20min;
(3) by cooling good culture medium in the barms access step (1) activated in step (2), regulate temperature at 29 ℃, cultivate 60-72h, until yeast quantity, reach at 1.0 hundred million o'clock, stop cultivating, make seed culture fluid standby;
(4) fermentation medium preparation, join the raw material of following percentage by weight in fermentation tank, corn steep liquor 55%, molasses 30%, water 15%, and stir, then being warming up to 100 ℃, insulation 30min carries out disinfection, and is cooled to room temperature after having sterilized standby;
(5) fermentation inoculation, the seed culture fluid that step (3) is made is squeezed in fermentation tank and is cultivated with pump, regulates temperature at 33 ℃, cultivates 40-60h;
(6) access lactic acid thalline is in fermentation, and until fermentation tank in, during sugar consumption 45%, the access lactic acid bacteria, control 33 ℃ of temperature, and sugar≤1.5% continues fermentation 50-60h, until sugared, has consumed;
(7) after fermentation ends, put tank, with maize peel, carry out Adsorption Concentration, obtain finished product after drying.
In step (5) and step (6), in sweat, pH is strict controlled in 5, will open and stir or pass into air stirring when adjusting pH, to guarantee the uniformity of pH.
In step (7), the moisture of dry rear finished product≤12%.
In step (7), the granularity >=0.5mm of finished product.
This day can be used 21600 tons of corn steep liquors every year after implementing, and amounts to skilful 1200 cubes of soak water of maize, can save 8640 tons of corns: solved starch factory soak water of maize emission problem simultaneously, also can make the raiser increase income, this production process produces without the three wastes, has good economic and social benefits.
Although illustrated and described embodiments of the invention, for the ordinary skill in the art, be appreciated that without departing from the principles and spirit of the present invention and can carry out multiple variation, modification, replacement and modification to these embodiment, scope of the present invention is limited by claims and equivalent thereof.
Claims (6)
1. a corn steep liquor is produced the method for vinasse protein feed, and it is characterized in that: the method comprises the following steps:
(1) culture medium preparation, join the raw material of following percentage by weight in culture tank corn steep liquor 25-35%, molasses 25-35%, water 30-50%; and stir; then be warming up to 100 ℃, insulation 30min carries out disinfection, and is cooled to room temperature after having sterilized standby;
(2) barms activation, put into warm water by barms, activated 20min;
(3) by cooling good culture medium in the barms access step (1) activate in step (2), regulate temperature at 28-30 ℃, cultivation 60-72h, reach 1.0 hundred million when above until yeast quantity, stops cultivation, makes seed culture fluid standby;
(4) fermentation medium preparation, join the raw material of following percentage by weight in fermentation tank corn steep liquor 50-60%, molasses 25-30%, water 10-20%; and stir; then be warming up to 100 ℃, insulation 30min carries out disinfection, and is cooled to room temperature after having sterilized standby;
(5) fermentation inoculation, the seed culture fluid that step (3) is made is squeezed in fermentation tank and is cultivated with pump, regulates temperature at 30-35 ℃, cultivates 40-60h;
(6) access lactic acid thalline ferments again, and until fermentation tank in, during sugar consumption 40-50%, the access lactic acid bacteria, control temperature 30-35 ℃, and sugar≤1.5% continues fermentation 50-60h, until sugared, has consumed;
(7) after fermentation ends, put tank, with maize peel, carry out Adsorption Concentration, pack to obtain finished product after drying.
2. the method for claim 1, it is characterized in that: in step (1), the raw material percentage of culture medium consists of: corn steep liquor 30%, molasses 30%, water 40%.
3. the method for claim 1, it is characterized in that: in step (4), the raw material percentage of fermentation medium consists of: corn steep liquor 55%, molasses 30%, water 15%.
4. the method for claim 1, it is characterized in that: in step (5) and step (6), in sweat, pH is strict controlled between 4.5-5.5, will open and stir or pass into air stirring when adjusting pH, to guarantee the uniformity of pH.
5. the method for claim 1 is characterized in that: in step (7), dry after the moisture≤12% of finished product.
6. the method for claim 1 is characterized in that: in step (7), and the granularity >=0.5mm of finished product.
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| CN2013104742415A CN103461654A (en) | 2013-10-12 | 2013-10-12 | Method for producing vinasse protein feed from corn steep liquor |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104719627A (en) * | 2015-04-13 | 2015-06-24 | 山东省鲁洲食品集团有限公司 | Method for processing corn steep liquor/dry powder capable of being directly fed to livestock |
| CN105104730A (en) * | 2015-08-28 | 2015-12-02 | 安徽丰原马鞍山生物化学有限公司 | Corn stillage DDGS feed |
| CN107484916A (en) * | 2017-09-29 | 2017-12-19 | 李春华 | A kind of processing method of fattening period chicken feed |
| CN108850548A (en) * | 2018-06-22 | 2018-11-23 | 河南飞天农业开发股份有限公司 | A kind of production technology for maize peel of whitewashing |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104719627A (en) * | 2015-04-13 | 2015-06-24 | 山东省鲁洲食品集团有限公司 | Method for processing corn steep liquor/dry powder capable of being directly fed to livestock |
| CN104719627B (en) * | 2015-04-13 | 2018-01-19 | 山东省鲁洲食品集团有限公司 | It is a kind of can Direct-fed livestock and poultry corn steep liquor/dry powder processing method |
| CN105104730A (en) * | 2015-08-28 | 2015-12-02 | 安徽丰原马鞍山生物化学有限公司 | Corn stillage DDGS feed |
| CN107484916A (en) * | 2017-09-29 | 2017-12-19 | 李春华 | A kind of processing method of fattening period chicken feed |
| CN108850548A (en) * | 2018-06-22 | 2018-11-23 | 河南飞天农业开发股份有限公司 | A kind of production technology for maize peel of whitewashing |
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Application publication date: 20131225 |