CN104431648A - Method for preparing sugarcane tail silage by aspergillus niger, candida tropicalis, bacillus subtilis and lactobacillus plantarum - Google Patents
Method for preparing sugarcane tail silage by aspergillus niger, candida tropicalis, bacillus subtilis and lactobacillus plantarum Download PDFInfo
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- CN104431648A CN104431648A CN201410710798.9A CN201410710798A CN104431648A CN 104431648 A CN104431648 A CN 104431648A CN 201410710798 A CN201410710798 A CN 201410710798A CN 104431648 A CN104431648 A CN 104431648A
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Abstract
The invention discloses a method for preparing sugarcane tail silage by aspergillus niger, candida tropicalis, bacillus subtilis and lactobacillus plantarum. The method comprises the following steps: shortly cutting mowed sugarcane tails to achieve the lengths being 1cm-4cm; uniformly spraying aspergillus niger, or aspergillus niger, candida tropicalis and bacillus subtilis, or a mixed preparation of aspergillus niger, candida tropicalis, bacillus subtilis and lactobacillus plantarum on fresh sugarcane tail raw materials so as to enable the contents of viable counts of aspergillus niger, candida tropicalis, bacillus subtilis and lactobacillus plantarum in the fresh sugarcane tails to reach 5.75*10<5>cfu/g-2.3*10<6>cfu/g, 3*10<5>cfu/g-4*10<5>cfu/g, 1.05*10<7>cfu/g-1.4*10<7>cfu/g and 3*10<6>cfu/g respectively; uniformly mixing; filling, compacting and sealing, wherein the density is 0.4kg/L; and preserving the product at room temperature for 40 days to prepare the sugarcane tail silage. According to the method disclosed by the invention, the problem that the palatability is poor when sugarcanes are directly used for feeding ruminants is solved, the content of lactic acid in the sugarcane tail silage is improved, and the content of acetic acid in the sugarcane tail silage is not improved.
Description
Technical field
The invention belongs to ensilage preparing technical field, specifically prepare the method for sugarcane tail ensiling with aspergillus niger, candida tropicalis, bacillus subtilis and Lactobacillus plantarum.
Background technology
The results of sugarcane have seasonality, it is reported, only Guangxi 2012-2013 squeezing season output is just more than 7,500 ten thousand tons, and sugarcane tail output accounts for sugarcane yield 1/4, is 1,875 ten thousand tons.The problem that sugarcane tail runs into as ruminant forage grass, one to be that sugarcane tail directly applies palatability poor, and two is seasons of sugarcane tail results, at a time when winter, not easily make dry forage grass, so, can effectively process these sugarcane tails and preserve animal husbandry development significant for a long time.It is the important means solving sugarcane tail long storage periods that ensiling makes, and not only can improve roughage quality, promotes the digestion of animal body, can also preserve for a long time and solve the problem that winter, ruminant feed lacked.
But so far, the document of application aspergillus niger, candida tropicalis, bacillus subtilis and Lactobacillus plantarum ensiling fresh cane tail aspect there is not yet report.
Summary of the invention
The object of this invention is to provide the method preparing the ensiling of sugarcane tail with aspergillus niger, candida tropicalis, bacillus subtilis and Lactobacillus plantarum.
The technical scheme that the present invention solves the problems of the technologies described above is as follows.
The microorganism fungus kind source of the present invention's application is as follows: aspergillus niger (Aspergillus niger, AN), candida tropicalis (Candida tropicalis.CT), bacillus subtilis (Bacillus subtilis, BS), Lactobacillus plantarum (Lactobacillus plantarum, LP) is all bought from Chinese industrial Microbiological Culture Collection administrative center.
Prepare the method for sugarcane tail ensiling with aspergillus niger, candida tropicalis, bacillus subtilis and Lactobacillus plantarum, operating procedure is as follows:
1. the collection of ensiling raw material:
Fresh cane tail after collection harvesting sugarcane is as raw material.
(2) shred:
Fresh cane tail is cut into 1cm ~ 4cm length respectively.
(3) by aspergillus niger, or aspergillus niger+candida tropicalis+bacillus subtilis, or aspergillus niger+candida tropicalis+bacillus subtilis+Lactobacillus plantarum mix preparation is evenly sprayed onto on the fresh cane tail raw material that shredded through step (2), make to reach 5.75 × 10 containing aspergillus niger viable count content in fresh cane tail
5cfu/g to 2.3 × 10
6cfu/g, candida tropicalis aspergillus viable count content reach 3 × 10
5cfu/g to 4 × 10
5cfu/g, bacillus subtilis aspergillus viable count content reach 1.05 × 10
7cfu/g to 1.4 × 10
7cfu/g, Lactobacillus plantarum viable count content reach 3 × 10
6cfu/g.
(4) filling, compacting, sealing:
Step (3) be with the addition of aspergillus niger, or aspergillus niger+candida tropicalis+bacillus subtilis, or the fresh cane tail successively compacting of aspergillus niger+candida tropicalis+bacillus subtilis+Lactobacillus plantarum mix preparation, be filled in closed container, density reaches more than 0.4kg/L, cover tightly lid, then carry out sealing preservation with sealed membrane.
(5) silage fermentation:
By step (4) filling, compacting, fresh cane tail silage fermentation under temperature 15 ~ 25 DEG C of conditions of sealing, fermentation time is more than 40 days.
(6) Product checking:
Fermented rear lactic acid content at more than 164mmol/kg, and acetic acid content is at 190mmol/kg) below.
(7) detect qualified after, airtight preservation is stand-by.
Beneficial effect of the present invention:
1. preparation method of the present invention is simple, and operating condition is easy, and raw material is easy to get.
2. the present invention by adding aspergillus niger in fresh cane tail ensilage, or aspergillus niger+candida tropicalis+bacillus subtilis, or aspergillus niger+candida tropicalis+bacillus subtilis+Lactobacillus plantarum effectively can improve sugarcane tail Silage Quality.
Detailed description of the invention
Below in conjunction with embodiment, the invention will be further described.
Following examples are only the schematic detailed description of the invention of the present invention, and are not used to limit scope of the present invention.Any those skilled in the art, must descend done equivalent variations, amendment and combine, all should belong to scope before not departing from design of the present invention and principle.
Embodiment 1
Prepare the method for sugarcane tail ensiling with aspergillus niger, operating procedure is as follows:
1. the collection of ensiling raw material:
Fresh cane tail after collection harvesting sugarcane is as raw material.
2. shred:
Fresh cane tail is cut into 1cm ~ 4cm length respectively.
3. black-koji mould liquid is evenly sprayed onto on the fresh cane tail raw material shredded through step (2), makes to reach 2.3 × 10 containing aspergillus niger viable count content in fresh cane tail
6cfu/g.
4. filling, compacting, sealing:
Step (3) be with the addition of the fresh cane tail successively compacting of black-koji mould liquid, be filled in closed container, density reaches more than 0.4kg/L, covers tightly lid, then carries out sealing preservation with sealed membrane.
5. silage fermentation:
By step (4) filling, compacting, fresh cane tail silage fermentation under temperature 15 ~ 25 DEG C of conditions of sealing, fermentation time is more than 40 days.
6. Product checking:
The rear lactic acid content that fermented is 244mmol/kg, and acetic acid content is 190mmol/kg;
7. detect qualified after, airtight preservation is stand-by.
The lactic acid content in the ensiling of kit measurement sugarcane tail is built up in application Nanjing; Acetic acid content in the ensiling of application gas chromatography determination sugarcane tail.
Embodiment 2
Prepare the method for sugarcane tail ensiling with aspergillus niger+candida tropicalis+bacillus subtilis, operating procedure is as follows:
1. the collection of ensiling raw material:
Fresh cane tail after collection harvesting sugarcane is as raw material.
2. shred:
Fresh cane tail is cut into 1cm ~ 4cm length respectively.
3. aspergillus niger+candida tropicalis+bacillus subtilis mix preparation is evenly sprayed onto on the fresh cane tail raw material shredded through step (2), makes to reach 7.7 × 10 containing aspergillus niger viable count content in fresh cane tail
5cfu/g, candida tropicalis viable count content reach 4 × 10
5cfu/g, bacillus subtilis viable count content reach 1.4 × 10
7cfu/g.
4. filling, compacting, sealing:
Step (3) be with the addition of the fresh cane tail successively compacting of aspergillus niger+candida tropicalis+bacillus subtilis mix preparation, be filled in closed container, density reaches more than 0.4kg/L, covers tightly lid, then carries out sealing preservation with sealed membrane.
5. silage fermentation:
By step (4) filling, compacting, fresh cane tail silage fermentation under temperature 15 ~ 25 DEG C of conditions of sealing, fermentation time is more than 40 days;
6. Product checking:
The rear lactic acid content that fermented is 164mmol/kg, and acetic acid content is 160mmol/kg;
7. detect qualified after, airtight preservation is stand-by.
Embodiment 3
Prepare the method for sugarcane tail ensiling with aspergillus niger+candida tropicalis+bacillus subtilis+Lactobacillus plantarum, operating procedure is as follows:
1. the collection of ensiling raw material:
Fresh cane tail after collection harvesting sugarcane is as raw material.
2. shred:
Fresh cane tail is cut into 1cm ~ 4cm length respectively.
3. aspergillus niger+candida tropicalis+bacillus subtilis+Lactobacillus plantarum mix preparation is evenly sprayed onto on the fresh cane tail raw material shredded through step (2), makes to reach 5.75 × 10 containing aspergillus niger viable count content in fresh cane tail
5cfu/g, candida tropicalis viable count content reach 3 × 10
5cfu/g, bacillus subtilis viable count content reach 1.05 × 10
7cfu/g and Lactobacillus plantarum viable count content reach 3 × 10
6cfu/g.
4. filling, compacting, sealing:
Step (3) be with the addition of the fresh cane tail successively compacting of aspergillus niger+candida tropicalis+bacillus subtilis+Lactobacillus plantarum mix preparation, be filled in closed container, density reaches more than 0.4kg/L, covers tightly lid, then carries out sealing preservation with sealed membrane.
5. silage fermentation:
By step (4) filling, compacting, fresh cane tail silage fermentation under temperature 15 ~ 25 DEG C of conditions of sealing, fermentation time is more than 40 days.
6. Product checking:
The rear lactic acid content that fermented is 211mmol/kg, and acetic acid content is 170mmol/kg;
7. detect qualified after, airtight preservation is stand-by.
Different additive on the impact of sugarcane tail silage fermentation quality in table 1.
Table 1 different additive is on the impact of sugarcane tail silage fermentation quality
Claims (1)
1. prepare the method for sugarcane tail ensiling with aspergillus niger, candida tropicalis, bacillus subtilis and Lactobacillus plantarum, it is characterized in that, operating procedure is as follows:
(1) collection of ensiling raw material:
Fresh cane tail after collection harvesting sugarcane is as raw material;
(2) shred:
Fresh cane tail is cut into 1cm ~ 4cm length respectively;
(3) by aspergillus niger, or aspergillus niger+candida tropicalis+bacillus subtilis, or aspergillus niger+candida tropicalis+bacillus subtilis+Lactobacillus plantarum mix preparation is evenly sprayed onto on the fresh cane tail raw material that shredded through step (2), make to reach 5.75 × 10 containing aspergillus niger viable count content in fresh cane tail
5cfu/g to 2.3 × 10
6cfu/g, candida tropicalis aspergillus viable count content reach 3 × 10
5cfu/g to 4 × 10
5cfu/g, bacillus subtilis aspergillus viable count content reach 1.05 × 10
7cfu/g to 1.4 × 10
7cfu/g, Lactobacillus plantarum viable count content reach 3 × 10
6cfu/g;
(4) filling, compacting, sealing:
Step (3) be with the addition of aspergillus niger, or aspergillus niger+candida tropicalis+bacillus subtilis, or the fresh cane tail successively compacting of aspergillus niger+candida tropicalis+bacillus subtilis+Lactobacillus plantarum mix preparation, be filled in closed container, density reaches more than 0.4kg/L, cover tightly lid, then carry out sealing preservation with sealed membrane;
(5) silage fermentation:
By step (4) filling, compacting, fresh cane tail silage fermentation under temperature 15 ~ 25 DEG C of conditions of sealing, fermentation time is more than 40 days;
(6) Product checking:
Fermented rear lactic acid content at more than 164mmol/kg, and acetic acid content is at 190mmol/kg) below;
(7) detect qualified after, airtight preservation is stand-by.
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| CN201410710798.9A CN104431648A (en) | 2014-11-28 | 2014-11-28 | Method for preparing sugarcane tail silage by aspergillus niger, candida tropicalis, bacillus subtilis and lactobacillus plantarum |
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| CN201410710798.9A CN104431648A (en) | 2014-11-28 | 2014-11-28 | Method for preparing sugarcane tail silage by aspergillus niger, candida tropicalis, bacillus subtilis and lactobacillus plantarum |
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Cited By (6)
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|---|---|---|---|---|
| CN106906145A (en) * | 2017-03-22 | 2017-06-30 | 上林县山水牛畜牧业有限公司 | A kind of probiotics and production method for the ensilage that ferments |
| CN108998387A (en) * | 2018-07-17 | 2018-12-14 | 广西大学 | A kind of lactic acid bacteria combination preparation and its application for sugarcane caudal lobe ensiling |
| CN109536416A (en) * | 2018-12-27 | 2019-03-29 | 南昌大学 | It is a kind of degrade feed with paper-mulberry leaf tannin silage inoculants bacteria preparation and its application method |
| CN109601760A (en) * | 2019-02-20 | 2019-04-12 | 常熟理工学院 | A kind of feed addictive, pomace feed and its preparation method and application |
| CN110100963A (en) * | 2019-05-05 | 2019-08-09 | 广西壮族自治区畜牧研究所 | A kind of Silaging method of sugarcane caudal lobe |
| CN114947009A (en) * | 2022-03-28 | 2022-08-30 | 河南科技学院 | Silage bacterial agent of triarrhena sacchariflora silage |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106906145A (en) * | 2017-03-22 | 2017-06-30 | 上林县山水牛畜牧业有限公司 | A kind of probiotics and production method for the ensilage that ferments |
| CN108998387A (en) * | 2018-07-17 | 2018-12-14 | 广西大学 | A kind of lactic acid bacteria combination preparation and its application for sugarcane caudal lobe ensiling |
| CN108998387B (en) * | 2018-07-17 | 2021-11-26 | 广西大学 | Lactobacillus combined preparation for ensiling sugarcane tail leaves and application thereof |
| CN109536416A (en) * | 2018-12-27 | 2019-03-29 | 南昌大学 | It is a kind of degrade feed with paper-mulberry leaf tannin silage inoculants bacteria preparation and its application method |
| CN109601760A (en) * | 2019-02-20 | 2019-04-12 | 常熟理工学院 | A kind of feed addictive, pomace feed and its preparation method and application |
| CN110100963A (en) * | 2019-05-05 | 2019-08-09 | 广西壮族自治区畜牧研究所 | A kind of Silaging method of sugarcane caudal lobe |
| CN110100963B (en) * | 2019-05-05 | 2022-08-30 | 广西壮族自治区畜牧研究所 | Silage method of sugarcane tail leaves |
| CN114947009A (en) * | 2022-03-28 | 2022-08-30 | 河南科技学院 | Silage bacterial agent of triarrhena sacchariflora silage |
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