CN104351588A - Method for preparing sugarcane tail silage by lactobacillus buchneri and lactobacillus plantarum - Google Patents
Method for preparing sugarcane tail silage by lactobacillus buchneri and lactobacillus plantarum Download PDFInfo
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- CN104351588A CN104351588A CN201410632953.XA CN201410632953A CN104351588A CN 104351588 A CN104351588 A CN 104351588A CN 201410632953 A CN201410632953 A CN 201410632953A CN 104351588 A CN104351588 A CN 104351588A
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Abstract
The invention discloses a method for preparing sugarcane tail silage by lactobacillus buchneri and lactobacillus plantarum. The method comprises the steps of cutting mowed sugarcane tails into short pieces with the lengths of 1-4cm; evenly spraying the mixed preparation of the lactobacillus plantarum and the lactobacillus buchneri onto the fresh sugarcane tail raw material to enable the viable count of the lactobacillus plantarum and the lactobacillus buchneri in the fresh sugarcane tails to reach 1*106cfu/g-2*106cfu/g, evenly mixing, filling, compacting and sealing, wherein the density of the mixture is 0.4kg/L; storing the product for 40 days at the normal temperature to obtain the sugarcane tail silage. After the method is adopted, the problem of poor palatability caused when a ruminant is directly fed with the sugarcane tails can be solved; the lactobacillus buchneri is combined with the lactobacillus plantarum for application, so that the content of lactic acid is improved, the content of acetic acid is reduced, and the utilization efficiency of water soluble carbohydrate (WSC) of the silage is improved.
Description
Technical field
The invention belongs to ensilage preparing technical field, specifically prepare the method for sugarcane tail ensilage with Bu Shi lactobacillus and Lactobacillus plantarum.
Background technology
The results of sugarcane have seasonality, it is reported, only Guangxi 2012-2013 squeezing season output is just more than 7,500 ten thousand tons, and sugarcane tail output accounts for sugarcane yield 1/4, is 1,875 ten thousand tons.The problem that sugarcane tail runs into as ruminant forage grass, one to be that sugarcane tail directly applies palatability poor, and two is seasons of sugarcane tail results, at a time when winter, not easily make dry forage grass, so, can effectively process these sugarcane tails and preserve animal husbandry development significant for a long time.It is the important means solving sugarcane tail long storage periods that ensiling makes, and not only can improve roughage quality, promotes the digestion of animal body, can also preserve for a long time and solve the problem that winter, ruminant feed lacked.
But so far, the document of use in conjunction Bu Shi lactobacillus and Lactobacillus plantarum ensiling fresh cane tail aspect there is not yet report.
Summary of the invention
The object of this invention is to provide the method preparing sugarcane tail ensilage with Bu Shi lactobacillus and Lactobacillus plantarum.
The technical scheme that the present invention solves the problems of the technologies described above is as follows.
The originating in lactic acid bacterium of the present invention's application is as follows: Bu Shi lactobacillus (Lactobasillus buchneri) and Lactobacillus plantarum (Lactobacillus plantarum) are all bought from Chinese industrial Microbiological Culture Collection administrative center.
Prepare the method for sugarcane tail ensilage with Bu Shi lactobacillus and Lactobacillus plantarum, operating procedure is as follows:
1. the collection of ensiling raw material:
Fresh cane tail after collection harvesting sugarcane is as raw material.
2. shred:
Fresh cane tail is cut into 1cm ~ 4cm length respectively.
3. add Bu Shi lactobacillus and Lactobacillus plantarum mix preparation:
Lactobacillus plantarum and Bu Shi lactobacillus mix preparation are evenly sprayed onto on fresh cane tail raw material, make all to reach 1 × 10 respectively containing Lactobacillus plantarum and Bu Shi living preparation of lactobacillus number content in fresh cane tail
6cfu/g to 2 × 10
6cfu/g.
4. filling, compacting, sealing:
Step 3 be with the addition of the fresh cane tail successively compacting of Bu Shi lactobacillus and Lactobacillus plantarum mix preparation, be filled in closed container, density reaches more than 0.4kg/L, covers tightly lid, then carries out sealing preservation with sealed membrane.
5. silage fermentation:
Step 4 is filled, compacting, fresh cane tail silage fermentation under temperature 15 ~ 25 DEG C of conditions of sealing, fermentation time is more than 40 days.
6. Product checking:
Fermented rear pH value below 4.32, and water-soluble carbohydrate content is at more than 9.85g/kg DM.
7. detect qualified after, airtight preservation is stand-by.
Above-mentioned lactic acid bacteria and Lactobacillus plantarum are originated as follows: Bu Shi lactobacillus (Lactobasillusbuchneri) and Lactobacillus plantarum (Lactobacillus plantarum) are all bought from Chinese industrial Microbiological Culture Collection administrative center.
Beneficial effect of the present invention:
1. preparation method of the present invention is simple, and operating condition is easy, and raw material is easy to get.
2. the present invention by adding Bu Shi lactobacillus and Lactobacillus plantarum effectively can improve sugarcane tail Silage Quality in fresh cane tail ensilage.
Detailed description of the invention
Below in conjunction with embodiment, the invention will be further described.
Following examples are only the schematic detailed description of the invention of the present invention, and are not used to limit scope of the present invention.Any those skilled in the art, must descend done equivalent variations, amendment and combine, all should belong to scope before not departing from design of the present invention and principle.
The originating in lactic acid bacterium of the present invention's application is as follows: Bu Shi lactobacillus (Lactobasillus buchneri) and Lactobacillus plantarum (Lactobacillus plantarum) are all bought from Chinese industrial Microbiological Culture Collection administrative center.
Embodiment 1
Prepare the method for sugarcane tail ensiling with Bu Shi lactobacillus and Lactobacillus plantarum, operating procedure is as follows:
1. the collection of ensiling raw material: the fresh cane tail after collection harvesting sugarcane is as raw material.
2. shred: fresh cane tail is cut into 1cm ~ 4cm length respectively.
3. add Bu Shi lactobacillus and Lactobacillus plantarum mix preparation: Lactobacillus plantarum and Bu Shi lactobacillus mix preparation are evenly sprayed onto on fresh cane tail raw material, make all to reach 1 × 10 respectively containing Lactobacillus plantarum and Bu Shi living preparation of lactobacillus number content in fresh cane tail
6cfu/g.
4. filling, compacting, sealing: the fresh cane tail successively compacting that step 3 be with the addition of Bu Shi lactobacillus and Lactobacillus plantarum mix preparation, be filled in closed container, density reaches more than 0.4kg/L, covers tightly lid, then carries out sealing preservation with sealed membrane.
5. silage fermentation: step 4 to be filled, compacting, fresh cane tail silage fermentation under temperature 15 ~ 25 DEG C of conditions of sealing, fermentation time is more than 40 days.
6. Product checking: rear pH value of having fermented is 4.26 ± 0.05, water-soluble carbohydrate content is 12.27 ± 1.01g/kgDM.
7. detect qualified after, airtight preservation is stand-by.
The lactic acid content in the ensiling of kit measurement sugarcane tail is built up in application Nanjing:
Soluble-carbohydrate (WSC) measures---anthrone colorimetry
Anthrone sulfuric acid solution: take 0.4g anthrone (analyzing pure) and be dissolved in 100ml 88% sulfuric acid (concentrated sulfuric acid of 84 parts of volumes mixes with the water of 16 parts of volumes).
Glucose solution: take 200mg glucose (analyzing pure) and be dissolved in 1000ml volumetric flask, be mixed with the standard liquid of 200ug/ml.In test tube, add 0 respectively, 0.1,0.2,0.3,0.4, the above-mentioned standard liquid of 0.5ml, in corresponding test tube, add 1.0 respectively, 0.9,0.8,0.7,0.6,0.5ml distilled water, corresponding concentration of glucose is respectively 0,20,40,60,80,100ug/ml.
Application spectrophotometer carries out colorimetric:
Determination step: the sample 0.2g taking pulverizing is placed in test tube, adds 10ml distilled water, boiling water bath 30min filters in 25ml, 50ml or 100ml volumetric flask, obtains sugared extract; Get the glucose standard of 1ml variable concentrations or sugared extract, add in test tube, add anthrone reagent 5ml, in boiling water bath, boil 10min, take out cooling, then under 620nm wavelength, measure OD value, according to the sugared content in Standard for Sugars curve calculation sample.
Embodiment 2
Prepare the method for sugarcane tail ensiling with Bu Shi lactobacillus and Lactobacillus plantarum, operating procedure is as follows:
1. the collection of ensiling raw material: the fresh cane tail after collection harvesting sugarcane is as raw material.
2. shred: fresh cane tail is cut into 1cm ~ 4cm length respectively.
3. add Bu Shi lactobacillus and Lactobacillus plantarum mix preparation: Lactobacillus plantarum and Bu Shi lactobacillus mix preparation are evenly sprayed onto on fresh cane tail raw material, make all to reach 2 × 10 respectively containing Lactobacillus plantarum and Bu Shi living preparation of lactobacillus number content in fresh cane tail
6.
4. filling, compacting, sealing: the fresh cane tail successively compacting that step 3 be with the addition of Bu Shi lactobacillus and Lactobacillus plantarum mix preparation, be filled in closed container, density reaches more than 0.4kg/L, covers tightly lid, then carries out sealing preservation with sealed membrane.
5. silage fermentation: step 4 to be filled, compacting, fresh cane tail silage fermentation under temperature 15 ~ 25 DEG C of conditions of sealing, fermentation time is more than 40 days;
6. Product checking: rear pH value of having fermented is 4.32 ± 0.20, water-soluble carbohydrate content is 11.86 ± 2.98g/kgDM.
7. detect qualified after, airtight preservation is stand-by.Detection method is identical with embodiment 1.
Different additive on the impact of sugarcane tail silage fermentation quality in table 1
Table 1 different additive is on the impact of sugarcane tail silage fermentation quality
Different additive on the impact of soluble-carbohydrate after the ensiling of sugarcane tail in table 2
Table 2 different additive is on the impact of soluble-carbohydrate after the ensiling of sugarcane tail
Claims (1)
1. prepare the method for sugarcane tail ensilage with Bu Shi lactobacillus and Lactobacillus plantarum, it is characterized in that, operating procedure is as follows:
(1) collection of ensiling raw material:
Fresh cane tail after collection harvesting sugarcane is as raw material;
(2) shred:
Fresh cane tail is cut into 1cm ~ 4cm length respectively;
(3) Bu Shi lactobacillus and Lactobacillus plantarum mix preparation is added:
Lactobacillus plantarum and Bu Shi lactobacillus mix preparation are evenly sprayed onto on fresh cane tail raw material, make all to reach 1 × 10 respectively containing Lactobacillus plantarum and Bu Shi living preparation of lactobacillus number content in fresh cane tail
6cfu/g to 2 × 10
6cfu/g;
(4) filling, compacting, sealing:
Step (3) be with the addition of the fresh cane tail successively compacting of Bu Shi lactobacillus and Lactobacillus plantarum mix preparation, be filled in closed container, density reaches more than 0.4kg/L, covers tightly lid, then carries out sealing preservation with sealed membrane;
(5) silage fermentation:
By step (4) filling, compacting, fresh cane tail silage fermentation under temperature 15 ~ 25 DEG C of conditions of sealing, fermentation time is more than 40 days;
(6) Product checking:
Fermented rear pH value below 4.32, and water-soluble carbohydrate content is at more than 9.85g/kg DM;
(7) detect qualified after, airtight preservation is stand-by.
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Cited By (10)
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| CN105146230A (en) * | 2015-11-02 | 2015-12-16 | 云南双胞胎饲料有限公司 | Pig feed additive for improving intestinal health and boosting immunity |
| CN105494918A (en) * | 2015-12-18 | 2016-04-20 | 广西壮族自治区水牛研究所 | Method for solid-state fermentation of cassava residuals achieved through combination of lactobacillus buchneri, aspergillus niger and lactobacillus plantarum |
| CN105505994A (en) * | 2015-12-16 | 2016-04-20 | 清华大学 | Comprehensive utilizing method of banana waste |
| CN105925618A (en) * | 2016-05-11 | 2016-09-07 | 杨新兰 | Applications of bacillus subtilis, lactobacillus buchneri and lactobacillus plantarum in co-fermentation of sugarcane leaves |
| CN106148249A (en) * | 2016-09-12 | 2016-11-23 | 江苏省农业科学院 | A kind of lactic acid bacteria microbial inoculum being applicable to grass silage and application thereof |
| CN107259127A (en) * | 2017-07-12 | 2017-10-20 | 华南农业大学 | Wooden ensilage of a kind of a variety of millet and preparation method and application |
| CN108330080A (en) * | 2017-11-14 | 2018-07-27 | 北京好实沃生物技术有限公司 | A kind of feed ensiling agent and its preparation method and application containing lactobacillus buchneri |
| CN108998387A (en) * | 2018-07-17 | 2018-12-14 | 广西大学 | A kind of lactic acid bacteria combination preparation and its application for sugarcane caudal lobe ensiling |
| CN110074260A (en) * | 2019-05-23 | 2019-08-02 | 广西龙州县亮剑生态牧业专业合作社 | A kind of method of sugarcane end pin production biological feedstuff |
| CN110100963A (en) * | 2019-05-05 | 2019-08-09 | 广西壮族自治区畜牧研究所 | A kind of Silaging method of sugarcane caudal lobe |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105146230A (en) * | 2015-11-02 | 2015-12-16 | 云南双胞胎饲料有限公司 | Pig feed additive for improving intestinal health and boosting immunity |
| CN105505994A (en) * | 2015-12-16 | 2016-04-20 | 清华大学 | Comprehensive utilizing method of banana waste |
| CN105494918A (en) * | 2015-12-18 | 2016-04-20 | 广西壮族自治区水牛研究所 | Method for solid-state fermentation of cassava residuals achieved through combination of lactobacillus buchneri, aspergillus niger and lactobacillus plantarum |
| CN105925618A (en) * | 2016-05-11 | 2016-09-07 | 杨新兰 | Applications of bacillus subtilis, lactobacillus buchneri and lactobacillus plantarum in co-fermentation of sugarcane leaves |
| CN106148249B (en) * | 2016-09-12 | 2019-09-06 | 江苏省农业科学院 | It is a kind of suitable for the lactic acid bacteria agent of grass silage and its application |
| CN106148249A (en) * | 2016-09-12 | 2016-11-23 | 江苏省农业科学院 | A kind of lactic acid bacteria microbial inoculum being applicable to grass silage and application thereof |
| CN107259127A (en) * | 2017-07-12 | 2017-10-20 | 华南农业大学 | Wooden ensilage of a kind of a variety of millet and preparation method and application |
| CN108330080A (en) * | 2017-11-14 | 2018-07-27 | 北京好实沃生物技术有限公司 | A kind of feed ensiling agent and its preparation method and application containing lactobacillus buchneri |
| CN108998387A (en) * | 2018-07-17 | 2018-12-14 | 广西大学 | A kind of lactic acid bacteria combination preparation and its application for sugarcane caudal lobe ensiling |
| CN108998387B (en) * | 2018-07-17 | 2021-11-26 | 广西大学 | Lactobacillus combined preparation for ensiling sugarcane tail leaves and application thereof |
| CN110100963A (en) * | 2019-05-05 | 2019-08-09 | 广西壮族自治区畜牧研究所 | A kind of Silaging method of sugarcane caudal lobe |
| CN110100963B (en) * | 2019-05-05 | 2022-08-30 | 广西壮族自治区畜牧研究所 | Silage method of sugarcane tail leaves |
| CN110074260A (en) * | 2019-05-23 | 2019-08-02 | 广西龙州县亮剑生态牧业专业合作社 | A kind of method of sugarcane end pin production biological feedstuff |
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Application publication date: 20150218 |