CN105925618A - Applications of bacillus subtilis, lactobacillus buchneri and lactobacillus plantarum in co-fermentation of sugarcane leaves - Google Patents
Applications of bacillus subtilis, lactobacillus buchneri and lactobacillus plantarum in co-fermentation of sugarcane leaves Download PDFInfo
- Publication number
- CN105925618A CN105925618A CN201610310679.3A CN201610310679A CN105925618A CN 105925618 A CN105925618 A CN 105925618A CN 201610310679 A CN201610310679 A CN 201610310679A CN 105925618 A CN105925618 A CN 105925618A
- Authority
- CN
- China
- Prior art keywords
- leaf
- sacchari sinensis
- caulis sacchari
- cfu
- lactobacillus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/225—Lactobacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/225—Lactobacillus
- C12R2001/25—Lactobacillus plantarum
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses applications of bacillus subtilis, lactobacillus buchneri and lactobacillus plantarum in co-fermentation of sugarcane leaves. In the applications, 1g of the dried sugarcane leaves has the living bacterium contents of bacillus subtilis, lactobacillus buchneri and lactobacillus plantarum as follows: 2.0x10<4> cfu/g, 1.0x10<5> cfu/g, and 3.5x10<6> cfu/g. The applications have the advantages that the steps are simple, and the popularization is easy; low-cost sugarcane leaves are taken as the main raw material, therefore, the income of sugarcane growers is increased, and meanwhile, the problem that the sugarcane growers burn away the sugarcane leaves which pollutes the environment is solved; the testing proves that the obtained fermented sample is good in sensory property, excellent in palatability, and high in nutritive value.
Description
Technical field
The present invention relates to leaf of Caulis Sacchari sinensis fermentation technical field, be specifically related to bacillus subtilis, Lactobacillus buchneri and Lactobacillus plantarum connection
Close the application of fermentation leaf of Caulis Sacchari sinensis.
Background technology
Leaf of Caulis Sacchari sinensis is the leaf of grass family saccharum plant Caulis Sacchari sinensis.Caulis Sacchari sinensis is extensively planted in the torrid zone and 100 Duo Ge states of subtropical zone
Family, grown worldwide area reaches more than 1,900 ten thousand hectares.China is cane planting big country, and the whole nation can produce Caulis Sacchari sinensis 15,000,000 every year
Ton, Guangxi is the main producing region of China Caulis Sacchari sinensis, and yield accounts for the 60% of the whole nation.But leaf of Caulis Sacchari sinensis produces as during cane planting
Agricultural waste material, although aboundresources, account for the 15% of Caulis Sacchari sinensis, but research and development be the fewest, raise except small part is used as animal
Material, major part is all incinerated and abandons, and causes the great wasting of resources and serious environmental pollution.
Leaf of Caulis Sacchari sinensis is directly used as animal feed and has that moisture height can not be stored for a long time, protein content is low and palatability is poor etc.
Defect.And make microorganism formulation after being fermented by leaf of Caulis Sacchari sinensis and can help raising dietary protein level, reduce crude fiber content.
At present, the fermentation of leaf of Caulis Sacchari sinensis mainly uses single bacterium to complete, and its fermentation quality does not obtain ultimate attainment performance.
Summary of the invention
The technical problem to be solved in the present invention is for above-mentioned the deficiencies in the prior art, it is provided that a kind of bacillus subtilis, Bu Shi breast
Bacillus and the application of Lactobacillus plantarum combined ferment leaf of Caulis Sacchari sinensis, leaf of Caulis Sacchari sinensis moisture height can not be stored for a long time, protein contains in solution
Measure the problems such as low and palatability is poor, significantly improve the fermentation quality of leaf of Caulis Sacchari sinensis.
The present invention solves above-mentioned technical problem and is adopted the technical scheme that: bacillus subtilis, Lactobacillus buchneri and plant breast bar
The application of bacterium combined ferment leaf of Caulis Sacchari sinensis, in described application, every gram dry bacillus subtilis in leaf of Caulis Sacchari sinensis, Lactobacillus buchneri and
The viable bacteria content of Lactobacillus plantarum is respectively 2.0 × 104cfu/g、1.0×105Cfu/g and 3.5 × 106cfu/g。
In described application, also adding carbamide and Testa oryzae in drying leaf of Caulis Sacchari sinensis, the addition of carbamide is for drying leaf of Caulis Sacchari sinensis gross mass
0.5-0.8%, the addition of Testa oryzae be dry leaf of Caulis Sacchari sinensis gross mass 3-5%.
In described application, fermentation condition is vacuum, normal temperature fermentation, and fermentation time is 10-15 days.
Step of the present invention is simple, easily promotes;With the leaf of Caulis Sacchari sinensis of low cost as main material, income can not only be increased for sugarcane grower, also
Solve sugarcane grower and burn the problem that leaf of Caulis Sacchari sinensis pollutes environment;After measured, the fermented sample of gained of the present invention, organoleptic properties is good, suitable
Mouth property is good, is of high nutritive value.
Detailed description of the invention
Below in conjunction with embodiment, technical scheme is described further.
Unless otherwise indicated, percentage ratio of the present invention is mass percent, and each constituent content percent sum is 100%.
One sample preparation
Embodiment 1
A kind of method utilizing mixed microorganism fermentation leaf of Caulis Sacchari sinensis, comprises the following steps:
(1) by fresh cane leaf segment, dry at temperature 55 ± 5 DEG C;
(2) adding carbamide and Testa oryzae in leaf of Caulis Sacchari sinensis after the drying, the addition of carbamide is dry leaf of Caulis Sacchari sinensis gross mass 0.5%,
The addition of Testa oryzae is dry leaf of Caulis Sacchari sinensis gross mass 5%;
(3) bacterium solution of bacillus subtilis, Lactobacillus buchneri and Lactobacillus plantarum is mixed, is seeded on the leaf of Caulis Sacchari sinensis of drying,
The viable bacteria content of bacillus subtilis, Lactobacillus buchneri and Lactobacillus plantarum in every gram of dry leaf of Caulis Sacchari sinensis is respectively
2.0×104cfu/g、1.0×105Cfu/g and 3.5 × 106cfu/g;
(4) step (3) being vaccinated with the leaf of Caulis Sacchari sinensis normal saline of mixed bacteria liquid and modulates water content is 40-50%, mixing
Uniformly, vacuum seals storage;Solid fermentation 10 days at normal temperatures, obtain leaf of Caulis Sacchari sinensis fermentation product samples 1#.
Embodiment 2
A kind of method utilizing mixed microorganism fermentation leaf of Caulis Sacchari sinensis, comprises the following steps:
(1) by fresh cane leaf segment, dry at temperature 55 ± 5 DEG C;
(2) adding carbamide and Testa oryzae in leaf of Caulis Sacchari sinensis after the drying, the addition of carbamide is dry leaf of Caulis Sacchari sinensis gross mass 0.8%,
The addition of Testa oryzae is dry leaf of Caulis Sacchari sinensis gross mass 3%;
(3) bacterium solution of bacillus subtilis, Lactobacillus buchneri and Lactobacillus plantarum is mixed, is seeded on the leaf of Caulis Sacchari sinensis of drying,
The viable bacteria content of bacillus subtilis, Lactobacillus buchneri and Lactobacillus plantarum in every gram of dry leaf of Caulis Sacchari sinensis is respectively
2.0×104cfu/g、1.0×105Cfu/g and 3.5 × 106cfu/g;
(4) step (3) being vaccinated with the leaf of Caulis Sacchari sinensis normal saline of mixed bacteria liquid and modulates water content is 40-50%, mixing
Uniformly, vacuum seals storage;Solid fermentation 10 days at normal temperatures, obtain leaf of Caulis Sacchari sinensis fermentation product samples 2#.
Embodiment 3
A kind of method utilizing mixed microorganism fermentation leaf of Caulis Sacchari sinensis, comprises the following steps:
(1) by fresh cane leaf segment, dry at temperature 55 ± 5 DEG C;
(2) adding carbamide and Testa oryzae in leaf of Caulis Sacchari sinensis after the drying, the addition of carbamide is dry leaf of Caulis Sacchari sinensis gross mass 0.6%,
The addition of Testa oryzae is dry leaf of Caulis Sacchari sinensis gross mass 4%;
(3) bacterium solution of bacillus subtilis, Lactobacillus buchneri and Lactobacillus plantarum is mixed, is seeded on the leaf of Caulis Sacchari sinensis of drying,
The viable bacteria content of bacillus subtilis, Lactobacillus buchneri and Lactobacillus plantarum in every gram of dry leaf of Caulis Sacchari sinensis is respectively
2.0×104cfu/g、1.0×105Cfu/g and 3.5 × 106cfu/g;
(4) step (3) being vaccinated with the leaf of Caulis Sacchari sinensis normal saline of mixed bacteria liquid and modulates water content is 40-50%, mixing
Uniformly, vacuum seals storage;Solid fermentation 15 days at normal temperatures, obtain leaf of Caulis Sacchari sinensis fermentation product samples 3#.
Embodiment 4
Without Testa oryzae, other steps are designated as 4# with embodiment 3, gained sample.
Embodiment 5
Without mixed bacteria liquid, other steps are designated as 5# with embodiment 3, gained sample.
Embodiment 6
The viable bacteria content adding the bacillus subtilis in every gram of dry leaf of Caulis Sacchari sinensis, Lactobacillus buchneri and Lactobacillus plantarum to is respectively
5.0×104cfu/g、1.0×106Cfu/g and 3.5 × 104Cfu/g, other steps are designated as 6# with embodiment 3, gained sample.
Embodiment 7
Without bacillus subtilis and Lactobacillus buchneri, other steps are designated as 7# with embodiment 3, gained sample.
Embodiment 8
Without Lactobacillus buchneri and Lactobacillus plantarum, other steps are designated as 8# with embodiment 3, gained sample.
Embodiment 9
Without Lactobacillus buchneri and bacillus subtilis, other steps are designated as 9# with embodiment 3, gained sample.
Embodiment 10
Reference examples: eliminate step (2) and (3), other steps are designated as 10# with embodiment 3, gained sample.
Two sample evaluations
Take 10 samples of above-mentioned gained the most respectively, first carry out the subjective appreciations such as its color and luster, abnormal smells from the patient and quality.Evaluation result is as follows
Shown in table:
2. take 10 samples of above-mentioned gained, according to the method in " forage analysis determination of feeds quality technology " literary composition, analyze and survey
Dry, crude protein, crude fat and crude fibre in random sample product.Result is as shown in the table:
Sample number into spectrum | Dry % | Crude protein % | Crude fat % | Crude fibre % |
1# | 95.91 | 11.28 | 2.88 | 25.25 |
2# | 96.77 | 10.60 | 2.92 | 26.87 |
3# | 96.56 | 10.35 | 2.92 | 25.62 |
4# | 93.40 | 8.14 | 2.14 | 33.54 |
5# | 95.04 | 7.40 | 2.06 | 35.60 |
6# | 94.75 | 7.70 | 2.34 | 33.19 |
7# | 93.50 | 6.56 | 2.08 | 31.41 |
8# | 92.82 | 6.41 | 2.06 | 32.54 |
9# | 93.73 | 6.83 | 2.06 | 33.78 |
10# | 94.29 | 6.84 | 2.12 | 33.75 |
Knowable to the data shown in above-mentioned two table, the fermented sample of gained of the present invention is utilized not only to there is advantage in terms of sense organ,
And achieve the most progressive in terms of nutritional labeling equally.
Claims (3)
1. bacillus subtilis, Lactobacillus buchneri and the application of Lactobacillus plantarum combined ferment leaf of Caulis Sacchari sinensis, it is characterised in that described
Application in, every gram of viable bacteria content drying bacillus subtilis in leaf of Caulis Sacchari sinensis, Lactobacillus buchneri and Lactobacillus plantarum is respectively
2.0×104cfu/g、1.0×105Cfu/g and 3.5 × 106cfu/g。
Application the most according to claim 1, it is characterised in that be also added with carbamide and Testa oryzae, carbamide in drying leaf of Caulis Sacchari sinensis
Addition be dry leaf of Caulis Sacchari sinensis gross mass 0.5-0.8%, the addition of Testa oryzae be dry leaf of Caulis Sacchari sinensis gross mass 3-5%.
Application the most according to claim 1 and 2, it is characterised in that fermentation condition is vacuum, normal temperature fermentation, during fermentation
Between be 10-15 days.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610310679.3A CN105925618A (en) | 2016-05-11 | 2016-05-11 | Applications of bacillus subtilis, lactobacillus buchneri and lactobacillus plantarum in co-fermentation of sugarcane leaves |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610310679.3A CN105925618A (en) | 2016-05-11 | 2016-05-11 | Applications of bacillus subtilis, lactobacillus buchneri and lactobacillus plantarum in co-fermentation of sugarcane leaves |
Publications (1)
Publication Number | Publication Date |
---|---|
CN105925618A true CN105925618A (en) | 2016-09-07 |
Family
ID=56835575
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201610310679.3A Pending CN105925618A (en) | 2016-05-11 | 2016-05-11 | Applications of bacillus subtilis, lactobacillus buchneri and lactobacillus plantarum in co-fermentation of sugarcane leaves |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN105925618A (en) |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104351588A (en) * | 2014-11-11 | 2015-02-18 | 广西壮族自治区水牛研究所 | Method for preparing sugarcane tail silage by lactobacillus buchneri and lactobacillus plantarum |
CN104431647A (en) * | 2014-11-18 | 2015-03-25 | 广西壮族自治区水牛研究所 | Method for preparing sugarcane sheath silage by using lactobacillus buchneri, candida tropicalis and bacillus subtilis |
-
2016
- 2016-05-11 CN CN201610310679.3A patent/CN105925618A/en active Pending
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104351588A (en) * | 2014-11-11 | 2015-02-18 | 广西壮族自治区水牛研究所 | Method for preparing sugarcane tail silage by lactobacillus buchneri and lactobacillus plantarum |
CN104431647A (en) * | 2014-11-18 | 2015-03-25 | 广西壮族自治区水牛研究所 | Method for preparing sugarcane sheath silage by using lactobacillus buchneri, candida tropicalis and bacillus subtilis |
Non-Patent Citations (4)
Title |
---|
唐振华等: "热带假丝酵母、枯草芽孢杆菌与乳酸菌组合对甘蔗尾青贮品质和有氧稳定性的影响", 《中国畜牧兽医》 * |
朱欣 等: "6种不同添加物对甘蔗叶梢青贮发酵品质的影响", 《草地学报》 * |
李梦楚等: "热带农业废弃物的饲料化利用研究进展", 《热带农业科学》 * |
穆胜龙等: "甘蔗尾饲料化研究进展", 《粮食与饲料工业》 * |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Lateef et al. | Improving the quality of agro-wastes by solid-state fermentation: enhanced antioxidant activities and nutritional qualities | |
Chen et al. | Effect of a low temperature tolerant lactic acid bacteria inoculant on the fermentation quality and bacterial community of oat round bale silage | |
CN106119171A (en) | The anti-microbial formulation of a kind of crop material feed fermentation and fermentation technology thereof | |
CN104397347B (en) | A kind of rapeseed meal fermented feed and preparation method thereof | |
CN104782892B (en) | A kind of sugarcane residue fermented feed and preparation method thereof | |
Peng et al. | Isolation, identification and utilization of lactic acid bacteria from silage in a warm and humid climate area | |
Du et al. | Use of Napier grass and rice straw hay as exogenous additive improves microbial community and fermentation quality of paper mulberry silage | |
CN105475637A (en) | Composite Chinese medicine residue microecological preparation and preparation method thereof | |
CN105851501A (en) | Mixed ensilage and preparation method thereof | |
CN105543131A (en) | Compound bacteria/cottonseed meal fermented feed and preparation method thereof | |
CN114107403B (en) | Method for co-producing ellagic acid and biological feed by fermenting pericarpium Granati with microbial community | |
CN112544787A (en) | Method for fermenting paper mulberry compound feed by bacteria and enzyme in synergy mode and compound feed thereof | |
CN108060103B (en) | A kind of Pediococcus pentosaceus and its application, screening calibration method | |
CN108060102B (en) | A kind of cibarium Wei Si Salmonella and its application, screening calibration method | |
de Araújo et al. | Fermentation dynamics, nutritional quality, and heating capacity of mixed silages of elephant grass (Pennisetum purpureum Schum) and Leucaena (Leucaena leucocephala) | |
CN105901283A (en) | Method for rapid fermentation preparation of silage | |
CN105925618A (en) | Applications of bacillus subtilis, lactobacillus buchneri and lactobacillus plantarum in co-fermentation of sugarcane leaves | |
CN109619270A (en) | A kind of preparation method of dry rice straw fermented feed | |
Dai et al. | Assessment of organic acid salts on fermentation quality, aerobic stability, and in vitro rumen digestibility of total mixed ration silage | |
CN109020754A (en) | A kind of organic compound fertilizer and preparation method thereof | |
CN107927353A (en) | It is a kind of suitable for lactic acid bacteria agent of green watermifoil ensiling and its preparation method and application | |
CN105851500A (en) | Dactylis glomerata-containing silage and preparation method thereof | |
CN103931887B (en) | A kind of broccoli stem-leaf fermented feed and preparation method thereof | |
Reis et al. | Fermentative profile and nutritional composition of sugarcane silages associated with different levels of moringa hay | |
CN105994980A (en) | Method for fermenting sugarcane leaves by utilizing mixed microbes |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
WD01 | Invention patent application deemed withdrawn after publication | ||
WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160907 |