CN105925618A - Applications of bacillus subtilis, lactobacillus buchneri and lactobacillus plantarum in co-fermentation of sugarcane leaves - Google Patents

Applications of bacillus subtilis, lactobacillus buchneri and lactobacillus plantarum in co-fermentation of sugarcane leaves Download PDF

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CN105925618A
CN105925618A CN201610310679.3A CN201610310679A CN105925618A CN 105925618 A CN105925618 A CN 105925618A CN 201610310679 A CN201610310679 A CN 201610310679A CN 105925618 A CN105925618 A CN 105925618A
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leaf
sacchari sinensis
caulis sacchari
cfu
lactobacillus
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杨新兰
韦玺
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/225—Lactobacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/225—Lactobacillus
    • C12R2001/25—Lactobacillus plantarum
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

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Abstract

The invention discloses applications of bacillus subtilis, lactobacillus buchneri and lactobacillus plantarum in co-fermentation of sugarcane leaves. In the applications, 1g of the dried sugarcane leaves has the living bacterium contents of bacillus subtilis, lactobacillus buchneri and lactobacillus plantarum as follows: 2.0x10<4> cfu/g, 1.0x10<5> cfu/g, and 3.5x10<6> cfu/g. The applications have the advantages that the steps are simple, and the popularization is easy; low-cost sugarcane leaves are taken as the main raw material, therefore, the income of sugarcane growers is increased, and meanwhile, the problem that the sugarcane growers burn away the sugarcane leaves which pollutes the environment is solved; the testing proves that the obtained fermented sample is good in sensory property, excellent in palatability, and high in nutritive value.

Description

Bacillus subtilis, Lactobacillus buchneri and the application of Lactobacillus plantarum combined ferment leaf of Caulis Sacchari sinensis
Technical field
The present invention relates to leaf of Caulis Sacchari sinensis fermentation technical field, be specifically related to bacillus subtilis, Lactobacillus buchneri and Lactobacillus plantarum connection Close the application of fermentation leaf of Caulis Sacchari sinensis.
Background technology
Leaf of Caulis Sacchari sinensis is the leaf of grass family saccharum plant Caulis Sacchari sinensis.Caulis Sacchari sinensis is extensively planted in the torrid zone and 100 Duo Ge states of subtropical zone Family, grown worldwide area reaches more than 1,900 ten thousand hectares.China is cane planting big country, and the whole nation can produce Caulis Sacchari sinensis 15,000,000 every year Ton, Guangxi is the main producing region of China Caulis Sacchari sinensis, and yield accounts for the 60% of the whole nation.But leaf of Caulis Sacchari sinensis produces as during cane planting Agricultural waste material, although aboundresources, account for the 15% of Caulis Sacchari sinensis, but research and development be the fewest, raise except small part is used as animal Material, major part is all incinerated and abandons, and causes the great wasting of resources and serious environmental pollution.
Leaf of Caulis Sacchari sinensis is directly used as animal feed and has that moisture height can not be stored for a long time, protein content is low and palatability is poor etc. Defect.And make microorganism formulation after being fermented by leaf of Caulis Sacchari sinensis and can help raising dietary protein level, reduce crude fiber content. At present, the fermentation of leaf of Caulis Sacchari sinensis mainly uses single bacterium to complete, and its fermentation quality does not obtain ultimate attainment performance.
Summary of the invention
The technical problem to be solved in the present invention is for above-mentioned the deficiencies in the prior art, it is provided that a kind of bacillus subtilis, Bu Shi breast Bacillus and the application of Lactobacillus plantarum combined ferment leaf of Caulis Sacchari sinensis, leaf of Caulis Sacchari sinensis moisture height can not be stored for a long time, protein contains in solution Measure the problems such as low and palatability is poor, significantly improve the fermentation quality of leaf of Caulis Sacchari sinensis.
The present invention solves above-mentioned technical problem and is adopted the technical scheme that: bacillus subtilis, Lactobacillus buchneri and plant breast bar The application of bacterium combined ferment leaf of Caulis Sacchari sinensis, in described application, every gram dry bacillus subtilis in leaf of Caulis Sacchari sinensis, Lactobacillus buchneri and The viable bacteria content of Lactobacillus plantarum is respectively 2.0 × 104cfu/g、1.0×105Cfu/g and 3.5 × 106cfu/g。
In described application, also adding carbamide and Testa oryzae in drying leaf of Caulis Sacchari sinensis, the addition of carbamide is for drying leaf of Caulis Sacchari sinensis gross mass 0.5-0.8%, the addition of Testa oryzae be dry leaf of Caulis Sacchari sinensis gross mass 3-5%.
In described application, fermentation condition is vacuum, normal temperature fermentation, and fermentation time is 10-15 days.
Step of the present invention is simple, easily promotes；With the leaf of Caulis Sacchari sinensis of low cost as main material, income can not only be increased for sugarcane grower, also Solve sugarcane grower and burn the problem that leaf of Caulis Sacchari sinensis pollutes environment；After measured, the fermented sample of gained of the present invention, organoleptic properties is good, suitable Mouth property is good, is of high nutritive value.
Detailed description of the invention
Below in conjunction with embodiment, technical scheme is described further.
Unless otherwise indicated, percentage ratio of the present invention is mass percent, and each constituent content percent sum is 100%.
One sample preparation
Embodiment 1
A kind of method utilizing mixed microorganism fermentation leaf of Caulis Sacchari sinensis, comprises the following steps:
(1) by fresh cane leaf segment, dry at temperature 55 ± 5 DEG C；
(2) adding carbamide and Testa oryzae in leaf of Caulis Sacchari sinensis after the drying, the addition of carbamide is dry leaf of Caulis Sacchari sinensis gross mass 0.5%, The addition of Testa oryzae is dry leaf of Caulis Sacchari sinensis gross mass 5%；
(3) bacterium solution of bacillus subtilis, Lactobacillus buchneri and Lactobacillus plantarum is mixed, is seeded on the leaf of Caulis Sacchari sinensis of drying, The viable bacteria content of bacillus subtilis, Lactobacillus buchneri and Lactobacillus plantarum in every gram of dry leaf of Caulis Sacchari sinensis is respectively 2.0×104cfu/g、1.0×105Cfu/g and 3.5 × 106cfu/g；
(4) step (3) being vaccinated with the leaf of Caulis Sacchari sinensis normal saline of mixed bacteria liquid and modulates water content is 40-50%, mixing Uniformly, vacuum seals storage；Solid fermentation 10 days at normal temperatures, obtain leaf of Caulis Sacchari sinensis fermentation product samples 1#.
Embodiment 2
A kind of method utilizing mixed microorganism fermentation leaf of Caulis Sacchari sinensis, comprises the following steps:
(1) by fresh cane leaf segment, dry at temperature 55 ± 5 DEG C；
(2) adding carbamide and Testa oryzae in leaf of Caulis Sacchari sinensis after the drying, the addition of carbamide is dry leaf of Caulis Sacchari sinensis gross mass 0.8%, The addition of Testa oryzae is dry leaf of Caulis Sacchari sinensis gross mass 3%；
(3) bacterium solution of bacillus subtilis, Lactobacillus buchneri and Lactobacillus plantarum is mixed, is seeded on the leaf of Caulis Sacchari sinensis of drying, The viable bacteria content of bacillus subtilis, Lactobacillus buchneri and Lactobacillus plantarum in every gram of dry leaf of Caulis Sacchari sinensis is respectively 2.0×104cfu/g、1.0×105Cfu/g and 3.5 × 106cfu/g；
(4) step (3) being vaccinated with the leaf of Caulis Sacchari sinensis normal saline of mixed bacteria liquid and modulates water content is 40-50%, mixing Uniformly, vacuum seals storage；Solid fermentation 10 days at normal temperatures, obtain leaf of Caulis Sacchari sinensis fermentation product samples 2#.
Embodiment 3
A kind of method utilizing mixed microorganism fermentation leaf of Caulis Sacchari sinensis, comprises the following steps:
(1) by fresh cane leaf segment, dry at temperature 55 ± 5 DEG C；
(2) adding carbamide and Testa oryzae in leaf of Caulis Sacchari sinensis after the drying, the addition of carbamide is dry leaf of Caulis Sacchari sinensis gross mass 0.6%, The addition of Testa oryzae is dry leaf of Caulis Sacchari sinensis gross mass 4%；
(3) bacterium solution of bacillus subtilis, Lactobacillus buchneri and Lactobacillus plantarum is mixed, is seeded on the leaf of Caulis Sacchari sinensis of drying, The viable bacteria content of bacillus subtilis, Lactobacillus buchneri and Lactobacillus plantarum in every gram of dry leaf of Caulis Sacchari sinensis is respectively 2.0×104cfu/g、1.0×105Cfu/g and 3.5 × 106cfu/g；
(4) step (3) being vaccinated with the leaf of Caulis Sacchari sinensis normal saline of mixed bacteria liquid and modulates water content is 40-50%, mixing Uniformly, vacuum seals storage；Solid fermentation 15 days at normal temperatures, obtain leaf of Caulis Sacchari sinensis fermentation product samples 3#.
Embodiment 4
Without Testa oryzae, other steps are designated as 4# with embodiment 3, gained sample.
Embodiment 5
Without mixed bacteria liquid, other steps are designated as 5# with embodiment 3, gained sample.
Embodiment 6
The viable bacteria content adding the bacillus subtilis in every gram of dry leaf of Caulis Sacchari sinensis, Lactobacillus buchneri and Lactobacillus plantarum to is respectively 5.0×104cfu/g、1.0×106Cfu/g and 3.5 × 104Cfu/g, other steps are designated as 6# with embodiment 3, gained sample.
Embodiment 7
Without bacillus subtilis and Lactobacillus buchneri, other steps are designated as 7# with embodiment 3, gained sample.
Embodiment 8
Without Lactobacillus buchneri and Lactobacillus plantarum, other steps are designated as 8# with embodiment 3, gained sample.
Embodiment 9
Without Lactobacillus buchneri and bacillus subtilis, other steps are designated as 9# with embodiment 3, gained sample.
Embodiment 10
Reference examples: eliminate step (2) and (3), other steps are designated as 10# with embodiment 3, gained sample.
Two sample evaluations
Take 10 samples of above-mentioned gained the most respectively, first carry out the subjective appreciations such as its color and luster, abnormal smells from the patient and quality.Evaluation result is as follows Shown in table:
2. take 10 samples of above-mentioned gained, according to the method in " forage analysis determination of feeds quality technology " literary composition, analyze and survey Dry, crude protein, crude fat and crude fibre in random sample product.Result is as shown in the table:
Sample number into spectrum Dry % Crude protein % Crude fat % Crude fibre %
1# 95.91 11.28 2.88 25.25
2# 96.77 10.60 2.92 26.87
3# 96.56 10.35 2.92 25.62
4# 93.40 8.14 2.14 33.54
5# 95.04 7.40 2.06 35.60
6# 94.75 7.70 2.34 33.19
7# 93.50 6.56 2.08 31.41
8# 92.82 6.41 2.06 32.54
9# 93.73 6.83 2.06 33.78
10# 94.29 6.84 2.12 33.75
Knowable to the data shown in above-mentioned two table, the fermented sample of gained of the present invention is utilized not only to there is advantage in terms of sense organ, And achieve the most progressive in terms of nutritional labeling equally.

Claims (3)

1. bacillus subtilis, Lactobacillus buchneri and the application of Lactobacillus plantarum combined ferment leaf of Caulis Sacchari sinensis, it is characterised in that described Application in, every gram of viable bacteria content drying bacillus subtilis in leaf of Caulis Sacchari sinensis, Lactobacillus buchneri and Lactobacillus plantarum is respectively 2.0×104cfu/g、1.0×105Cfu/g and 3.5 × 106cfu/g。
Application the most according to claim 1, it is characterised in that be also added with carbamide and Testa oryzae, carbamide in drying leaf of Caulis Sacchari sinensis Addition be dry leaf of Caulis Sacchari sinensis gross mass 0.5-0.8%, the addition of Testa oryzae be dry leaf of Caulis Sacchari sinensis gross mass 3-5%.
Application the most according to claim 1 and 2, it is characterised in that fermentation condition is vacuum, normal temperature fermentation, during fermentation Between be 10-15 days.
CN201610310679.3A 2016-05-11 2016-05-11 Applications of bacillus subtilis, lactobacillus buchneri and lactobacillus plantarum in co-fermentation of sugarcane leaves Pending CN105925618A (en)

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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104351588A (en) * 2014-11-11 2015-02-18 广西壮族自治区水牛研究所 Method for preparing sugarcane tail silage by lactobacillus buchneri and lactobacillus plantarum
CN104431647A (en) * 2014-11-18 2015-03-25 广西壮族自治区水牛研究所 Method for preparing sugarcane sheath silage by using lactobacillus buchneri, candida tropicalis and bacillus subtilis

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104351588A (en) * 2014-11-11 2015-02-18 广西壮族自治区水牛研究所 Method for preparing sugarcane tail silage by lactobacillus buchneri and lactobacillus plantarum
CN104431647A (en) * 2014-11-18 2015-03-25 广西壮族自治区水牛研究所 Method for preparing sugarcane sheath silage by using lactobacillus buchneri, candida tropicalis and bacillus subtilis

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
唐振华等: "热带假丝酵母、枯草芽孢杆菌与乳酸菌组合对甘蔗尾青贮品质和有氧稳定性的影响", 《中国畜牧兽医》 *
朱欣 等: "6种不同添加物对甘蔗叶梢青贮发酵品质的影响", 《草地学报》 *
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Application publication date: 20160907