CN106417898B - A kind of preparation method and applications for the microorganism formulation improving cotton stalk and cotton seed hulls efficiency of feed utilization - Google Patents
A kind of preparation method and applications for the microorganism formulation improving cotton stalk and cotton seed hulls efficiency of feed utilization Download PDFInfo
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Abstract
The invention belongs to Animal Science feed processing research fields, and more particularly to a kind of microorganism formulation for improving cotton stalk and cotton seed hulls utilization rate, which is made of micro organism powder A and additive B；The method applied it on processing cotton stalk and cotton seed hulls includes the following steps: that (1) takes 30-50 parts of the additive B cotton stalks that 500-2000 parts of crushing are added and/or cotton seed hulls to mix, clean water is sprayed by 60% ~ 70% water content, it is uniformly mixed, pile small pile type, shading net is covered, is stacked 7 days naturally；(2) 10-50 parts of micro organism powder A are added, mixes, mixture is paved into high 10 centimetres, wide 30 centimetres of strip structure, covers shading net, stack 30 days naturally, during which spray clean water daily, keep the moisture content in mixture between 60% ~ 70%；It can be obtained the feed for mainly containing cotton stalk and/or cotton seed hulls.With this product treated cotton stalk and the feeding of/cotton seed hulls after sheep, the significant utilization rate for improving cotton stalk and/cotton seed hulls.
Description
Technical field
The invention belongs to Animal Science feed processing research fields, more particularly to one kind for improving cotton stalk and cottonseed
Shell is as the preparation method of the microorganism formulation of the utilization rate of feed and its for improving cotton stalk and cotton seed hulls feed conversion
The application of rate.
Background technique
Cotton stalk and cotton seed hulls contain the energy of more than half that photosynthesis of plant is accumulated, and are rich in raw sugar, thick egg
Ingredient needed for white matter etc. is conducive to domestic animals and fowls is especially used to feed the ruminant domestic animals such as ox, sheep, still for feeding livestock and poultry
Can so become good feed resource, but because its wood fibre cellulose content is high, containing the limitation such as free gossypol and palatability difference because
Element, is directly used as that efficiency of feed utilization is low, effect is poor, hinders the comprehensive utilization of cotton stalk and cotton seed hulls scale.Microorganism
Facture can be acted on by microbial fermentation, can be by cellulose, hemicellulose and the lignin in cotton stalk and cotton seed hulls point
Solution is further oxidized to CO at monosaccharide, disaccharides2, feeding can be improved after domestic animal cotton stalk and cotton seed hulls digestibility or
DM in the rumen degradation rate, to improve the utilization rate of cotton stalk and cotton seed hulls.
Summary of the invention
It is an object of the invention to: it is a kind of for handling the microorganism formulation of cotton stalk and cotton seed hulls by researching and developing, it uses
After the feed for mainly containing cotton stalk and cotton seed hulls of this microorganism formulation preparation is delivered to the domestic animals such as sheep, can significantly it mention
Utilization rate of the domestic animals such as high sheep to cotton stalk and cotton seed hulls.
Technical solution of the present invention: 1, the microorganism formulation of cotton stalk and cotton seed hulls utilization rate, the microorganism system are improved
Agent is made of micro organism powder A and additive B, wherein micro organism powder A's the preparation method comprises the following steps: by whiterot fungi, black
Aspergillus, gibberella, rod method mould and Penicillium notatum at least two or two or more 0.5-1 parts of combinations are inoculated in and have sterilized
In 50-100 parts of PD culture solutions, seed liquor is made in the shaken cultivation 5 days under conditions of 25 DEG C, 150r/min；Seed liquor is used
Sterile water is serially diluted to 10-3After CFU/mL, 5-10 parts of dilutions are taken to be inoculated in 100-300 parts of PD culture solutions, 25
DEG C, under conditions of 150r/min by strain culturing to logarithmic phase, draw 40-80 part culture solutions and 20-60 parts of wheat bran, 10-40 parts
Dregs of beans, 10-40 portion corn flour, 5-10 portions of defatted rice brans, 5-10 parts of corn protein powders mix, and the microbial mixture of mixing is cold
Dry rear obtained micro organism powder A is lyophilized, measuring its viable count is 1.9 × 108CFU/g is fitted into tinfoil paper paper bag, is sealed after vacuumizing
Mouthful, it is placed in 4 DEG C of preservations；The additive B is made of 10-25 parts of quick lime, 10-15 parts of sucrose.
2, a kind of that above-mentioned microorganism formulation is applied in cotton stalk and cotton seed hulls feed, under which includes
It states step: (1) taking 30-50 parts of the additive B cotton stalks that 500-2000 parts of crushing are added and/or cotton seed hulls to mix, by 60%
~70% water content sprays clean water, is uniformly mixed, piles small pile type, cover shading net, stacks 7 days naturally；(2) to step
(1) 10-50 parts of micro organism powder A are added in, mixes, mixture is paved into high 10 centimetres, wide 30 centimetres of strip structure, is covered
Shading net is stacked 30 days naturally, during which sprays clean water on strip mixture daily, keeps the moisture content in mixture
Between 60%~70%；It can be obtained the feed for mainly containing cotton stalk and/or cotton seed hulls.
Among the above, whiterot fungi, black-koji mould, gibberella, rod method mould, Penicillium notatum, wheat bran, dregs of beans, corn flour, degreasing
Rice bran, corn protein powder, quick lime, sucrose, cotton stalk and cotton seed hulls are commercially available.
Beneficial effects of the present invention:, as experimental animal, to feed microbiological treatment under same trophic level in sheep
Cotton stalk and/cotton seed hulls afterwards, carry out Digestion And Metabolism In Sheep test, weigh surplus material, the urine for collecting sheep, excrement, measurement
Fiber content in sheep fine fodder, cotton stalk and/cotton seed hulls show that feeding this product can be mentioned obviously by analyzing the data obtained
High cotton stalk and cotton seed hulls utilization rate.The mechanism of action: cotton stalk and cellulose, hemicellulose and wooden in/cotton seed hulls
Element in microorganism under the synergistic effect of beta-glucosidase, exoglucanase, endoglucanase, can be converted into monosaccharide,
Disaccharides is further oxidized to CO2, with this microorganism formulation treated cotton stalk and the feeding of/cotton seed hulls after sheep,
Experiment shows that the utilization rate of cotton stalk and/cotton seed hulls can be significantly improved.
Specific embodiment
The present invention is further described in conjunction with the embodiments.
Practical proof: embodiment 1 prepares feed S1, and one, prepare micro organism powder A: respectively by whiterot fungi, black-koji mould
Each 0.8 part is inoculated in the 80 parts of PD culture solutions to have sterilized, the shaken cultivation 5 days under conditions of 25 DEG C, 150r/min, is made
Seed liquor, it is spare；Seed liquor is serially diluted using sterile water to 10-3After CFU/mL, 7 parts of dilutions is taken to be inoculated in 200
In part PD culture solution, by strain culturing to logarithmic phase under conditions of 25 DEG C, 150r/min, 50 parts of culture solutions and 25 portions of wheats are drawn
Bran, 15 parts of dregs of beans, 15 portions of corn flour, 10 portions of defatted rice brans, 10 parts of corn protein powders mix 30-40min, by the microorganism of mixing
Micro organism powder A is made after mixture freeze-drying, measuring its viable count is 1.9 × 108CFU/g is fitted into tinfoil paper paper bag, is taken out
It is sealed after vacuum, is placed in 4 DEG C of preservations；Two, (1) takes 30 parts of the additive B cotton stalks and/or cottonseed that 500 parts of crushing are added
Shell mixes, and sprays clean water by 60%~70% water content, is uniformly mixed, piles small pile type, cover shading net, natural heap
It puts 7 days；(2) to 30 parts of micro organism powder A are added in step (1), mix, by mixture be paved into high 10 centimetres, it is 30 centimetres wide
Strip structure covers shading net, stacks 30 days naturally, during which sprays clean water on strip mixture daily, keeps mixing
Feed S1 is made between 60%~70% in moisture content in object.
Embodiment 2 prepares feed S2, and one, prepare micro organism powder A: respectively by whiterot fungi, each 0.8 part of inoculation of gibberella
In the 80 parts of PD culture solutions to have sterilized, seed liquor is made in the shaken cultivation 5 days under conditions of 25 DEG C, 150r/min, standby
With；Seed liquor is serially diluted using sterile water to 10-3After CFU/mL, 7 parts of dilutions is taken to be inoculated in 200 parts of PD culture solutions
In, by strain culturing to logarithmic phase under conditions of 25 DEG C, 150r/min, draw 50 parts of culture solutions and 25 parts of wheat bran, 15 parts of beans
The dregs of rice, 15 portions of corn flour, 10 portions of defatted rice brans, 10 parts of corn protein powders mix 30-40min, and the microbial mixture of mixing is cold
Dry rear obtained micro organism powder A is lyophilized, measuring its viable count is 1.9 × 108CFU/g is fitted into tinfoil paper paper bag, is sealed after vacuumizing
Mouthful, it is placed in 4 DEG C of preservations；Two, (1) takes 30 parts of the additive B cotton stalks and/or cotton seed hulls that 1000 parts of crushing are added, and mixes,
Clean water is sprayed by 60%~70% water content, is uniformly mixed, piles small pile type, cover shading net, stack 7 days naturally；(2)
To 30 parts of micro organism powder A are added in step (1), mix, mixture is paved into high 10 centimetres, wide 30 centimetres of strip structure,
Shading net is covered, is stacked 30 days naturally, during which sprays clean water on strip mixture daily, keeps the moisture in mixture
Feed S2 is made between 60%~70% in content.
Embodiment 3 prepares feed S3: one, prepares micro organism powder A: respectively by each 0.8 part of whiterot fungi, rod method enzyme bacterium
It is inoculated in the 80 parts of PD culture solutions to have sterilized, the shaken cultivation 5 days under conditions of 25 DEG C, 150r/min, seed liquor is made,
It is spare；Seed liquor is serially diluted using sterile water to 10-3After CFU/mL, 7 parts of dilutions is taken to be inoculated in 200 parts of PD cultures
In liquid, by strain culturing to logarithmic phase under conditions of 25 DEG C, 150r/min, 50 parts of culture solutions and 25 parts of wheat bran, 15 parts are drawn
Dregs of beans, 15 portions of corn flour, 10 portions of defatted rice brans, 10 parts of corn protein powders mix 30-40min, and the microorganism of mixing is mixed
Micro organism powder A is made after closing object freeze-drying, measuring its viable count is 1.9 × 108CFU/g is fitted into tinfoil paper paper bag, is taken out true
It is sealed after sky, is placed in 4 DEG C of preservations；Two, (1) takes 30 parts of the additive B cotton stalks and/or cotton seed hulls that 2000 parts of crushing are added,
It mixes, sprays clean water by 60%~70% water content, be uniformly mixed, pile small pile type, cover shading net, stack 7 naturally
It；(2) 30 parts of micro organism powder A are added into step (1), mixes, mixture is paved into high 10 centimetres, wide 30 centimetres of strip
Structure covers shading net, stacks 30 days naturally, during which sprays clean water on strip mixture daily, keeps in mixture
Moisture content between 60%~70%, be made feed S3.
Embodiment 4 prepares feed S4: one, prepares micro organism powder A: respectively by whiterot fungi, each 0.8 part of inoculation of Penicillium notatum
In the 80 parts of PD culture solutions to have sterilized, seed liquor is made in the shaken cultivation 5 days under conditions of 25 DEG C, 150r/min, standby
With.Seed liquor is serially diluted using sterile water to 10-3After CFU/mL, 7 parts of dilutions is taken to be inoculated in 200 parts of PD culture solutions
In, by strain culturing to logarithmic phase under conditions of 25 DEG C, 150r/min, draw 50 parts of culture solutions and 25 parts of wheat bran, 15 parts of beans
The dregs of rice, 15 portions of corn flour, 10 portions of defatted rice brans, 10 parts of corn protein powders mix 30-40min, and the microorganism of mixing is mixed
Micro organism powder is made after object freeze-drying, measuring its viable count is 1.9 × 108CFU/g is fitted into tinfoil paper paper bag, vacuumizes
After seal, be placed in 4 DEG C of preservations；Two, (1) takes 30 parts of the additive B cotton stalks and/or cotton seed hulls that 1500 parts of crushing are added, and mixes
It is even, clean water is sprayed by 60%~70% water content, is uniformly mixed, piles small pile type, cover shading net, stack 7 days naturally；
(2) 30 parts of micro organism powder A are added into step (1), mixes, mixture is paved into high 10 centimetres, wide 30 centimetres of strip knot
Structure covers shading net, stacks 30 days naturally, during which sprays clean water on strip mixture daily, keeps in mixture
Feed S4 is made between 60%~70% in moisture content.
Implement component design and compliance test result for feed S1, S2, S3 and S4:
1 materials and methods
1.1 test periods and place
This test is carried out in May, 2016 in August, 2016 in Xinjiang Huaxing breeding stock Breeding Center.
1.2 experimental animals and experimental design
Test chooses similar in age, weight Tao Saite ewe 50, is randomly divided into 5 groups, respectively control group, test I
IV group of group, II group of test, III group of test and test.Control group and test group pottery Saite ewe feed same trophic level and same
The fine fodder of dosage.Every ewe 07:00 every morning and 17:00 in afternoon feed 250g fine fodder respectively, after searching for food to fine fodder,
Control group, I group of test, II group of test, III group of test and IV group of test are common respectively at daily 07:00 and 17:00 in afternoon feeding
Cotton stalk, each 1kg of feed S1, S2, S3 and S4, while guaranteeing sufficient drinking-water.20 days by a definite date digest supersession tests are carried out,
Wherein preliminary trial period 13 days, the positive examination phase 7 days.
The acquisition and measurement of 2 samples
Ewe whole day is set to keep standing state in the positive examination phase, using self-control urine collection apparatus, the every 2h of whole day collects ewe urine
It is primary with excrement sample, whole day urine is sufficiently shaken up, total volume is measured with graduated cylinder and takes 10%, be stored in after 5% concentrated sulfuric acid is added
Plastic bottle, Seal and preservation simultaneously make a record, and will test the urine sample that ewe 7 days collect and mix, take 1L to save to be measured；By the complete of collection
Its excrement sample mixes completely, random to grab the 10% of excrement sample gross weight, is put into numbered sample sack, weighs after natural air drying.It will
The excrement samples of the continuous 7 days natural air dryings of ewe mixes, take 1kg perform record seal up for safekeeping it is to be measured；It is collected simultaneously fine fodder, common cotton chopped straw
Stalk, feed S1, S2, S3 and S4.
Dry matter, organic matter, calcium, phosphorus contain in fine fodder, common cotton stalk, feed S1, S2, S3 and S4, excrement sample and urine sample
Amount is all made of conventional feed analysis method and is measured；Neutral detergent fiber and acid detergent fiber content use U.S. ANKOM
Fibre analyser is measured；Gross protein value is surveyed using the quick azotometer of Germany Elementar Analysen Systeme
It is fixed.
The analysis of 3 data
Test result is indicated with average (Mean) ± standard deviation (SD).Test data is all made of the progress of SPSS18.0 software
Variance analysis.Test result carries out Multiple range test using Duncan.
4 results and analysis
The influence of feeding feed stuff S1, S2, S3 and S4 to Tao Saite ewe nutritive digestibility is shown in Table 1.
Data shoulder mark difference lowercase letter indication difference of going together is significant (P < 0.05), and shoulder mark difference capitalization indicates poor
Heteropolar significant (P < 0.01), shoulder mark same letter or not significant (P > 0.05) without letter expression difference.
As shown in Table 1, each test group dry matter, organic matter digestibility are above control group, reach wherein testing III group
Extremely significant level (P < 0.01)；Each group thick protein digestibility difference is not significant (P > 0.05), but each test group is above pair
According to group；Each test group cellulose digestibility is above control group, wherein test II group it is extremely significant be higher than control group (P < 0.01),
Test I group, test II group, test III group, test IV group of cellulose digestibility be higher by 8.17% (P > than control group respectively
0.05), 11.22% (P < 0.01), 6.75% (P > 0.05), 8.01% (P > 0.05)；Test I group of hemicellulose digestibility
It is significantly higher than control group (P < 0.05), IV group of I group of test, II group of test, III group of test, test hemicellulose digestibility difference
13.20% (P < 0.05), 11.71% (P < 0.05), 5.73% (P > 0.05), 7.96% (P > are higher by than control group
0.05)；Each group calcium, phosphorus digestibility difference is not significant (P > 0.05), but each test group calcium, phosphorus digestibility are above control group.
Claims (1)
1. a kind of application method that microorganism formulation is applied in cotton stalk and cotton seed hulls feed, which is characterized in that this is answered
Include the following steps: one with method, prepare micro organism powder A: whiterot fungi, each 0.8 part of gibberella being inoculated in sterilized respectively
In 80 parts of good PD culture solutions, seed liquor is made in the shaken cultivation 5 days under conditions of 25 DEG C, 150 r/min, spare；It will kind
Sub- liquid is serially diluted using sterile water to 10-3After CFU/mL, 7 parts of dilutions are taken to be inoculated in 200 parts of PD culture solutions, In
25 DEG C, under conditions of 150r/min by strain culturing to logarithmic phase, draw 50 parts of culture solutions and 25 parts of wheat bran, 15 parts of dregs of beans, 15
Part corn flour, 10 portions of defatted rice brans, 10 parts of corn protein powders mix 30-40 min, the microbial mixture of mixing are freezed dry
Obtained micro organism powder A after dry, measuring its viable count is 1.9 × 108 CFU/g is fitted into tinfoil paper paper bag, is sealed after vacuumizing,
It is placed in 4 DEG C of preservations；Two, (1) takes 30 parts of the additive B cotton stalks and/or cotton seed hulls that 1000 parts of crushing are added, and mixes, presses
60% ~ 70% water content sprays clean water, is uniformly mixed, piles small pile type, cover shading net, stacks 7 days naturally；(2) to step
Suddenly 30 parts of micro organism powder A are added in (1), mixes, mixture is paved into high 10 centimetres, wide 30 centimetres of strip structure, is covered
Shading net is stacked 30 days naturally, during which sprays clean water on strip mixture daily, keeps the moisture content in mixture
Between 60% ~ 70%, feed is made.
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| CN110313542A (en) * | 2019-08-06 | 2019-10-11 | 塔里木大学 | A method of fermented feed is prepared using cotton byproduct |
| CN110637919A (en) * | 2019-09-18 | 2020-01-03 | 新和县园邦城农业发展有限公司 | Novel straw composite feed of yellow mealworm sand and ghost |
| CN110833115A (en) * | 2019-11-21 | 2020-02-25 | 南京胜昔实业有限公司 | Comprehensive utilization method of cotton production by-products by using biochemical fulvic acid as main product |
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