CN104306463B - 一种用苦荞米下脚料制备苦荞总黄酮的方法及其应用 - Google Patents
一种用苦荞米下脚料制备苦荞总黄酮的方法及其应用 Download PDFInfo
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- CN104306463B CN104306463B CN201410616474.9A CN201410616474A CN104306463B CN 104306463 B CN104306463 B CN 104306463B CN 201410616474 A CN201410616474 A CN 201410616474A CN 104306463 B CN104306463 B CN 104306463B
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Abstract
本发明公开一种用苦荞米下脚料制备苦荞总黄酮的方法及其应用,将苦荞麸皮经三次提取,再将提取液分别趁热过滤,所得滤液分别经常压回收乙醇,再分别浓缩后放置晶析,滤出晶体即得苦荞总黄酮粗品,在苦荞总黄酮粗品中加入水或无水乙醇,再加热至沸腾,然后趁热滤过,再将滤液放置晶析,直至析出总黄酮,然后洗涤、干燥,即得到苦荞总黄酮。所得苦荞总黄酮用于包括***片在内的保健品。本发明提供的方法效率高、成本低、生产期短,所得苦荞总黄酮中芦丁含量80~90%,其它黄酮如檞皮素等占10~15%,制备的***片具有多方面的保健功能。
Description
技术领域
本发明属于食药技术领域,具体涉及一种用苦荞米下脚料制备苦荞总黄酮的方法及其应用。
背景技术
苦荞是谷类作物中唯一集七大营养素于一身的作物,其七大营养素包括:1)生物类黄酮、2)微量元素和矿物质、3)淀粉、4)维生素、5)纤维素、6)脂肪、7)蛋白质。其中,生物类黄酮的主要成份是芦丁,又名维生素P。其主要功效是:软化血管,改善微循环,清热解毒、活血化瘀、拔毒生肌、有降血糖、尿糖、血脂、益气提神、加强胰岛素外周作用。而芦丁在其它谷物中几乎没有。另外,苦荞还含有其它粮食作物不具备的叶绿素。
苦荞食品具有明显的降低血脂、血糖、血压的三降作用,还能防治糖尿病、冠心病。
现有提取苦荞麦黄酮类成分的方法已有多种,诸如乙醇回流提取法、热水回流法、煎煮法、饱和石灰水(饱和氢氧化钙水液)提取酚沉淀法、乙醇超声波提取法,碱水超声波提取法等。上述方法各有优点缺点,现简单分析如下:
1、60~90%乙醇回流提取方法:提取效果较好,然而此法最大的问题是乙醇消耗量偏大,在整个提取和回收乙醇的过程中根据设备的性能不同,含有20~40%的乙醇损失,并且还产生了相应能源的多余消耗,生产成本较高。
2、热水提取法:此法不消耗乙醇等,但是提取效率偏低一些,同时受荞麦中淀粉等多糖糊化的影响,固液分离较困难,这也使总黄酮的提取效率下降,而且生产工期延长。
3、饱和氢氧化钙水溶液(或者氢氧化钠)调节pH=8~9的水溶液加热提取法,此法不消耗乙醇,但是也有多糖糊化等问题,而且试验发现,有少量黄酮苷(或芦丁)发生水解,由此降低了总黄酮成分的收得率,是其弊端。
4、乙醇超声波提取法,此法消耗的乙醇更多,由于受提取温度限制,对于芦丁来说,成本高使得此法不适用于大规模生产。
5、碱性水(氢氧化钙或氢氧化钠水溶液)的超生提取法,此法成本虽不高,但受提取罐(或槽)容积的限制较难施展,同时与上述第三种方法一样是在碱性条件下,有少量黄酮苷(或芦丁)被水解,有损失,且芦丁苷之檞皮素本身不容于水或碱水,故残留于原料中,造成损失。另外,碱水提取过程若加了硼砂及亚硫酸钠来防止水解反应及氧化反应的发生,则提取过的“渣”即麸皮的再利用价值就大为降低或无价值了。
因此,有必要研发一种高效率、低成本提取苦荞麦黄酮类成分的方法。
发明内容
为解决现有技术成本高、效率低等问题,本发明提供一种用苦荞米下脚料制备苦荞总黄酮的方法及其应用,以苦荞麦加工苦荞全营养米后得到的下脚料(麸皮)为原料,提取芦丁占总黄酮90%的苦荞麦黄铜。
本发明通过下列技术方案实现:一种用苦荞米下脚料制备苦荞总黄酮的方法,经过下列各步骤:
(1)将苦荞麸皮加入5~8体积倍的浓度为45~55v/v%的乙醇进行加热回流提取2~4小时,滤出提取液Ⅰ;再在剩余物中加入4~6体积倍的浓度为30~35v/v%的乙醇进行加热回流提取2~4小时,滤出提取液Ⅱ;再在剩余物中加入5~7质量倍的水进行加热回流提取0.5~1.5小时,滤出提取液Ⅲ;合并提取液Ⅰ和提取液Ⅱ得提取液Ⅳ;
(2)将步骤(1)所得提取液Ⅳ和提取液Ⅲ分别趁热过滤,所得滤液分别经常压回收乙醇,再分别浓缩后放置晶析,滤出晶体即得苦荞总黄酮粗品;
(3)在步骤(2)所得苦荞总黄酮粗品中加入水或体积浓度为80%的乙醇,再加热至沸腾,保持微沸1~3分钟,然后趁热滤过,再将滤液放置晶析,直至析出总黄酮,然后用去离子水或纯水洗涤总黄酮,至洗出液颜色为无色或接近无色时,再经抽滤或脱水,然后在60℃以下进行干燥,即得到苦荞总黄酮。所得苦荞总黄酮的得率为80~90%,其中芦丁的含量占黄酮总量的85~90%。
所述步骤(2)和(3)中放置晶析的母液及步骤(1)滤出提取液Ⅲ后剩余的苦荞麸皮用于制备常规饮料及膳食纤维食品。
本发明的提取工艺中,第一次提取后,经测定苦荞总黄酮的提取率为原料麸皮中含量的50~55%,此次提取之所以用体积浓度为45~55%的乙醇是经反复试验及测定,优选出的保证了提取效率的合适浓度,如加大乙醇浓度,提取效率会再增加,但乙醇的耗量及回收乙醇时产生的损失就明显增加,且拖长了回收乙醇的时间,从成本和效率的比来说是体积浓度为45~55%、用量5~8倍时为最高,即效率/成本在此范围是一较平滑的拐点。第二次提取所得苦荞黄铜占原料麸皮原来含量的25~30%。第三次提取所得苦荞黄铜占原料麸皮原来含量的8~12%,因提取得率不高,且水提液中有粘液质等多糖成分,不与第1、2次合并,以减少干扰。在滤液回收乙醇及浓缩时,提取液Ⅲ因其中仍含有近10%的乙醇,故避免损失仍先回收乙醇至无醇味后再单独浓缩至放置析晶。
本发明的另一目的在于提供所得苦荞总黄酮用于制备***片的方法,经过下列各步骤:
A、制备总黄酮β-环糊精包结物:将苦荞总黄酮粉碎至60~80目,再按苦荞总黄酮与β-环糊精的质量比为1:(5~6),在苦荞总黄酮中加入β-环糊精,然后加入β-环糊精8~10倍质量的水并加热至45~50℃,再经超声处理或者搅拌25~30分钟后冷至室温,静置4小时后过滤,将沉淀物在80℃以下干燥,得总黄酮β-环糊精包结物;
或者,按苦荞总黄酮与β-环糊精的质量比为1:(5~6),在苦荞总黄酮中加入β-环糊精,再球磨1小时,得总黄酮β-环糊精包结物;
B、取总黄酮β-环糊精包结物质量1.5~2%的阿斯巴甜和50~60%的微晶纤维素,将阿斯巴甜按等量递加法混匀在微晶纤维素中,得到混合物Ⅰ,将混合物Ⅰ与步骤(1)所得总黄酮β-环糊精包结物按等量递加法混匀,得到混合物Ⅱ;然后在混合物Ⅱ中加入总黄酮β-环糊精包结物1~1.5倍质量的糊精进行混匀,得到混合物Ⅲ;
C、在步骤B所得的混合物Ⅲ中加入总黄酮β-环糊精包结物3~4倍质量的木糖醇,再加入总黄酮β-环糊精包结物质量50~60%的羟丙基甲基纤维素,并混合均匀得到混合物Ⅳ;
D、取总黄酮β-环糊精包结物质量5~8%的羧甲基纤维素钠和0~0.1%的食用色素,将羧甲基纤维素钠与食用色素混合,再按固液比g/mL计为(3~5):100加水制成色胶浆;
E、在步骤C所得混合物Ⅳ中加入步骤D所得色胶浆以常规湿法制成颗粒,再在60~70℃干燥至含水量为15~25%,再加入干燥后颗粒质量1%的硬脂酸镁混合均匀,然后压成片状,即得到***片,每片含苦荞总黄酮10~20mg。
所述步骤C的木糖醇粉碎至80目以上。
按常规制成***片的片重为0.8±0.05g,每片含苦荞总黄酮10~20mg。
本发明具备的优点和效果:本发明提供的方法效率高、成本低、生产期短,所得苦荞总黄酮中芦丁含量近80~90%,其它黄酮如檞皮素等占10~15%,提取后的剩余麸皮仍含有蛋白质、碳水化合物、膳食纤维等营养成分,仍可再经下一步合理有效利用,以实现价值最大化。本发明提供的***片先将苦荞总黄酮制备为β-环糊精包结物以改善口感,并且提高本品保健治疗作用的生物利用度;再以阿斯巴甜加上木糖醇作为甜味剂以适应不能或不宜吃糖的人食用。由于富含芦丁及其苷之檞皮素,具有多方面的保健功能,特别对糖尿病及心血管疾病具有降血脂、降血糖的辅助治疗作用。
具体实施方式
下面通过实施例对本发明做进一步说明。
实施例1
(1)将苦荞麸皮加入5体积倍的浓度为50v/v%的乙醇进行加热回流提取2小时,滤出提取液Ⅰ;再在剩余物中加入5体积倍的浓度为35v/v%的乙醇进行加热回流提取4小时,滤出提取液Ⅱ;再在剩余物中加入6质量倍的水进行加热回流提取1小时,滤出提取液Ⅲ;合并提取液Ⅰ和提取液Ⅱ得提取液Ⅳ;
(2)将步骤(1)所得提取液Ⅳ和提取液Ⅲ分别趁热过滤,所得滤液分别经常压回收乙醇,再分别浓缩后放置晶析,滤出晶体即得苦荞总黄酮粗品;
(3)在步骤(2)所得苦荞总黄酮粗品中加入水或体积浓度为80%的乙醇,再加热至沸腾,保持微沸分钟,然后趁热滤过,再将滤液放置晶析,直至析出总黄酮,然后用去离子水洗涤总黄酮,至洗出液颜色为无色或接近无色时,再经抽滤,然后在60℃以下进行干燥,即得到苦荞总黄酮。所得苦荞总黄酮的得率为88%,其中芦丁的含量占黄酮总量的90%。
步骤(2)和(3)中放置晶析的母液及步骤(1)滤出提取液Ⅲ后剩余的苦荞麸皮用于制备常规饮料及膳食纤维食品。
所得苦荞总黄酮用于制备***片,经过下列各步骤:
A、制备总黄酮β-环糊精包结物:将苦荞总黄酮粉碎至80目,再按苦荞总黄酮与β-环糊精的质量比为1:5,在苦荞总黄酮中加入β-环糊精,然后加入β-环糊精9倍质量的水并加热至55℃,再经超声处理或者搅拌25分钟后冷至室温,静置4小时后过滤,将沉淀物在80℃以下干燥,得总黄酮β-环糊精包结物;
B、取总黄酮β-环糊精包结物质量2%的阿斯巴甜和55%的微晶纤维素,将阿斯巴甜按等量递加法混匀在微晶纤维素中,得到混合物Ⅰ,将混合物Ⅰ与步骤(1)所得总黄酮β-环糊精包结物按等量递加法混匀,得到混合物Ⅱ;然后在混合物Ⅱ中加入总黄酮β-环糊精包结物1.5倍质量的糊精进行混匀,得到混合物Ⅲ;
C、在步骤B所得的混合物Ⅲ中加入总黄酮β-环糊精包结物3倍质量的80目以上木糖醇,再加入总黄酮β-环糊精包结物质量60%的羟丙基甲基纤维素,并混合均匀得到混合物Ⅳ;
D、取总黄酮β-环糊精包结物质量6%的羧甲基纤维素钠,再按固液比g/mL计为5:100加水制成色胶浆;
E、在步骤C所得混合物Ⅳ中加入步骤D所得色胶浆以常规湿法制成颗粒,再在60℃干燥至含水量为20%,再加入干燥后颗粒质量1%的硬脂酸镁混合均匀,然后压成片状,即得到***片,每片含苦荞总黄酮10~20mg。
实施例2
(1)将苦荞麸皮加入8体积倍的浓度为55v/v%的乙醇进行加热回流提取3小时,滤出提取液Ⅰ;再在剩余物中加入6体积倍的浓度为32v/v%的乙醇进行加热回流提取3小时,滤出提取液Ⅱ;再在剩余物中加入7质量倍的水进行加热回流提取1.5小时,滤出提取液Ⅲ;合并提取液Ⅰ和提取液Ⅱ得提取液Ⅳ;
(2)将步骤(1)所得提取液Ⅳ和提取液Ⅲ分别趁热过滤,所得滤液分别经常压回收乙醇,再分别浓缩后放置晶析,滤出晶体即得苦荞总黄酮粗品;
(3)在步骤(2)所得苦荞总黄酮粗品中加入水或体积浓度为80%的乙醇,再加热至沸腾,保持微沸1分钟,然后趁热滤过,再将滤液放置晶析,直至析出总黄酮,然后用纯水洗涤总黄酮,至洗出液颜色为无色或接近无色时,再经脱水,然后在50℃进行干燥,即得到苦荞总黄酮。所得苦荞总黄酮的得率为90%,其中芦丁的含量占黄酮总量的89%。
步骤(2)和(3)中放置晶析的母液及步骤(1)滤出提取液Ⅲ后剩余的苦荞麸皮用于制备常规饮料及膳食纤维食品。
所得苦荞总黄酮用于制备***片,经过下列各步骤:
A、制备总黄酮β-环糊精包结物:将苦荞总黄酮粉碎至70目,再按苦荞总黄酮与β-环糊精的质量比为1:6,在苦荞总黄酮中加入β-环糊精,然后加入β-环糊精10倍质量的水并加热至50℃,再经超声处理或者搅拌28分钟后冷至室温,静置4小时后过滤,将沉淀物在60℃干燥,得总黄酮β-环糊精包结物;
B、取总黄酮β-环糊精包结物质量1.5%的阿斯巴甜和60%的微晶纤维素,将阿斯巴甜按等量递加法混匀在微晶纤维素中,得到混合物Ⅰ,将混合物Ⅰ与步骤(1)所得总黄酮β-环糊精包结物按等量递加法混匀,得到混合物Ⅱ;然后在混合物Ⅱ中加入总黄酮β-环糊精包结物1.2倍质量的糊精进行混匀,得到混合物Ⅲ;
C、在步骤B所得的混合物Ⅲ中加入总黄酮β-环糊精包结物4倍质量的100目木糖醇,再加入总黄酮β-环糊精包结物质量55%的羟丙基甲基纤维素,并混合均匀得到混合物Ⅳ;
D、取总黄酮β-环糊精包结物质量8%的羧甲基纤维素钠和0.1%的食用色素,将羧甲基纤维素钠与食用色素混合,再按固液比g/mL计为4:100加水制成色胶浆;
E、在步骤C所得混合物Ⅳ中加入步骤D所得色胶浆以常规湿法制成颗粒,再在65℃干燥至含水量为25%,再加入干燥后颗粒质量1%的硬脂酸镁混合均匀,然后压成片状,即得到***片,每片含苦荞总黄酮10~20mg。
实施例3
(1)将苦荞麸皮加入6体积倍的浓度为45v/v%的乙醇进行加热回流提取4小时,滤出提取液Ⅰ;再在剩余物中加入4体积倍的浓度为30v/v%的乙醇进行加热回流提取2小时,滤出提取液Ⅱ;再在剩余物中加入5质量倍的水进行加热回流提取0.5小时,滤出提取液Ⅲ;合并提取液Ⅰ和提取液Ⅱ得提取液Ⅳ;
(2)将步骤(1)所得提取液Ⅳ和提取液Ⅲ分别趁热过滤,所得滤液分别经常压回收乙醇,再分别浓缩后放置晶析,滤出晶体即得苦荞总黄酮粗品;
(3)在步骤(2)所得苦荞总黄酮粗品中加入水或体积浓度为80%的乙醇,再加热至沸腾,保持微沸2分钟,然后趁热滤过,再将滤液放置晶析,直至析出总黄酮,然后用去离子水或纯水洗涤总黄酮,至洗出液颜色为无色或接近无色时,再经抽滤或脱水,然后在45℃下进行干燥,即得到苦荞总黄酮。所得苦荞总黄酮的得率为87%,其中芦丁的含量占黄酮总量的90%。
步骤(2)和(3)中放置晶析的母液及步骤(1)滤出提取液Ⅲ后剩余的苦荞麸皮用于制备常规饮料及膳食纤维食品。
所得苦荞总黄酮用于制备***片,经过下列各步骤:
A、制备总黄酮β-环糊精包结物:将苦荞总黄酮粉碎至60目,再按苦荞总黄酮与β-环糊精的质量比为1:5,在苦荞总黄酮中加入β-环糊精,然后加入β-环糊精8倍质量的水并加热至45℃,再经超声处理或者搅拌30分钟后冷至室温,静置4小时后过滤,将沉淀物在60℃下干燥,得总黄酮β-环糊精包结物;
B、取总黄酮β-环糊精包结物质量1.8%的阿斯巴甜和50%的微晶纤维素,将阿斯巴甜按等量递加法混匀在微晶纤维素中,得到混合物Ⅰ,将混合物Ⅰ与步骤(1)所得总黄酮β-环糊精包结物按等量递加法混匀,得到混合物Ⅱ;然后在混合物Ⅱ中加入总黄酮β-环糊精包结物1倍质量的糊精进行混匀,得到混合物Ⅲ;
C、在步骤B所得的混合物Ⅲ中加入总黄酮β-环糊精包结物4倍质量的90目以上木糖醇,再加入总黄酮β-环糊精包结物质量50%的羟丙基甲基纤维素,并混合均匀得到混合物Ⅳ;
D、取总黄酮β-环糊精包结物质量5%的羧甲基纤维素钠和0.05%的食用色素,将羧甲基纤维素钠与食用色素混合,再按固液比g/mL计为3:100加水制成色胶浆;
E、在步骤C所得混合物Ⅳ中加入步骤D所得色胶浆以常规湿法制成颗粒,再在70℃干燥至含水量为15%,再加入干燥后颗粒质量1%的硬脂酸镁混合均匀,然后压成片状,即得到***片,每片含苦荞总黄酮10~20mg。
实施例4
(1)将苦荞麸皮加入8体积倍的浓度为55v/v%的乙醇进行加热回流提取3小时,滤出提取液Ⅰ;再在剩余物中加入6体积倍的浓度为32v/v%的乙醇进行加热回流提取3小时,滤出提取液Ⅱ;再在剩余物中加入7质量倍的水进行加热回流提取1.5小时,滤出提取液Ⅲ;合并提取液Ⅰ和提取液Ⅱ得提取液Ⅳ;
(2)将步骤(1)所得提取液Ⅳ和提取液Ⅲ分别趁热过滤,所得滤液分别经常压回收乙醇,再分别浓缩后放置晶析,滤出晶体即得苦荞总黄酮粗品;
(3)在步骤(2)所得苦荞总黄酮粗品中加入水或体积浓度为80%的乙醇,再加热至沸腾,保持微沸1分钟,然后趁热滤过,再将滤液放置晶析,直至析出总黄酮,然后用纯水洗涤总黄酮,至洗出液颜色为无色或接近无色时,再经脱水,然后在50℃进行干燥,即得到苦荞总黄酮。所得苦荞总黄酮的得率为90%,其中芦丁的含量占黄酮总量的89%。
步骤(2)和(3)中放置晶析的母液及步骤(1)滤出提取液Ⅲ后剩余的苦荞麸皮用于制备常规饮料及膳食纤维食品。
所得苦荞总黄酮用于制备***片,经过下列各步骤:
A、制备总黄酮β-环糊精包结物:按苦荞总黄酮与β-环糊精的质量比为1:5,在苦荞总黄酮中加入β-环糊精,再球磨1小时,得总黄酮β-环糊精包结物;
B、取总黄酮β-环糊精包结物质量1.5%的阿斯巴甜和60%的微晶纤维素,将阿斯巴甜按等量递加法混匀在微晶纤维素中,得到混合物Ⅰ,将混合物Ⅰ与步骤(1)所得总黄酮β-环糊精包结物按等量递加法混匀,得到混合物Ⅱ;然后在混合物Ⅱ中加入总黄酮β-环糊精包结物1.2倍质量的糊精进行混匀,得到混合物Ⅲ;
C、在步骤B所得的混合物Ⅲ中加入总黄酮β-环糊精包结物4倍质量的100目木糖醇,再加入总黄酮β-环糊精包结物质量55%的羟丙基甲基纤维素,并混合均匀得到混合物Ⅳ;
D、取总黄酮β-环糊精包结物质量8%的羧甲基纤维素钠和0.1%的食用色素,将羧甲基纤维素钠与食用色素混合,再按固液比g/mL计为4:100加水制成色胶浆;
E、在步骤C所得混合物Ⅳ中加入步骤D所得色胶浆以常规湿法制成颗粒,再在65℃干燥至含水量为25%,再加入干燥后颗粒质量1%的硬脂酸镁混合均匀,然后压成片状,即得到***片,每片含苦荞总黄酮10~20mg。
取上述实施例所得***片,用于下列保健治疗:
一、治疗对象
糖尿病50例;高血压50例;高血脂50例;抵抗力低50例;冠心病50例。
将上述各类病例随机平分为保健组和对照组各125例。
二、用法
保健组按常规治疗方法服药,并服用上述实施例所得***片,一次1~2g,一日3次,连服60日进行观察。
对照组按常规治疗方法服药,不服用保健品。
三、结果
本发明提供的***片临床保健效果:显效率达100%,对糖尿病有效率达85%;对高血压有效率达90%;对高血脂有效率达88%;对抵抗力低有效率达92%;对冠心病有效率达86%。
而对照组对糖尿病有效率达65%;对高血压有效率达63%;对高血脂有效率达37%;对抵抗力低有效率达70%;对冠心病有效率达59%。
四、结论
本发明提供的***片富含芦丁及其苷之檞皮素,对糖尿病及心血管疾病具有降血脂、降血糖的辅助治疗作用,其辅助作用优于普通治疗的对照组。
该***片完全利用植物提取纯化,原料易获得,有保健作用佳、应用安全、毒副作用小等优点,所制得的产品质量稳定,使用方便,无任何副作用。
Claims (3)
1.一种苦荞总黄酮制备***片的方法,其特征在于经过下列各步骤:
A、制备总黄酮β-环糊精包结物:将苦荞总黄酮粉碎至60~80目,再按苦荞总黄酮与β-环糊精的质量比为1:(5~6),在苦荞总黄酮中加入β-环糊精,然后加入β-环糊精8~10倍质量的水并加热至45~50℃,再经超声处理或者搅拌25~30分钟后冷至室温,静置4小时后过滤,将沉淀物在80℃以下干燥,得总黄酮β-环糊精包结物;
或者,按苦荞总黄酮与β-环糊精的质量比为1:(5~6),在苦荞总黄酮中加入β-环糊精,再球磨1小时,得总黄酮β-环糊精包结物;
其中,苦荞总黄酮用苦荞米下脚料制备,具体经过下列各步骤:
(1)将苦荞麸皮加入5~8体积倍的浓度为45~55v/v%的乙醇进行加热回流提取2~4小时,滤出提取液Ⅰ;再在剩余物中加入4~6体积倍的浓度为30~35v/v%的乙醇进行加热回流提取2~4小时,滤出提取液Ⅱ;再在剩余物中加入5~7质量倍的水进行加热回流提取0.5~1.5小时,滤出提取液Ⅲ;合并提取液Ⅰ和提取液Ⅱ得提取液Ⅳ;
(2)将步骤(1)所得提取液Ⅳ和提取液Ⅲ分别趁热过滤,所得滤液分别经常压回收乙醇,再分别浓缩后放置晶析,滤出晶体即得苦荞总黄酮粗品;
(3)在步骤(2)所得苦荞总黄酮粗品中加入水或体积浓度为80%的乙醇,再加热至沸腾,保持微沸1~3分钟,然后趁热滤过,再将滤液放置晶析,直至析出总黄酮,然后用去离子水或纯水洗涤总黄酮,至洗出液颜色为无色或接近无色时,再经抽滤或脱水,然后在60℃以下进行干燥,即得到苦荞总黄酮;
B、取总黄酮β-环糊精包结物质量1.5~2%的阿斯巴甜和50~60%的微晶纤维素,将阿斯巴甜按等量递加法混匀在微晶纤维素中,得到混合物Ⅰ,将混合物Ⅰ与步骤(1)所得总黄酮β-环糊精包结物按等量递加法混匀,得到混合物Ⅱ;然后在混合物Ⅱ中加入总黄酮β-环糊精包结物1~1.5倍质量的糊精进行混匀,得到混合物Ⅲ;
C、在步骤B所得的混合物Ⅲ中加入总黄酮β-环糊精包结物3~4倍质量的木糖醇,再加入总黄酮β-环糊精包结物质量50~60%的羟丙基甲基纤维素,并混合均匀得到混合物Ⅳ;
D、取总黄酮β-环糊精包结物质量5~8%的羧甲基纤维素钠和0~0.1%的食用色素,将羧甲基纤维素钠与食用色素混合,再按固液比g/mL计为(3~5):100加水制成色胶浆;
E、在步骤C所得混合物Ⅳ中加入步骤D所得色胶浆以常规湿法制成颗粒,再在60~70℃干燥至含水量为15~25%,再加入干燥后颗粒质量1%的硬脂酸镁混合均匀,然后压成片状,即得到***片。
2.根据权利要求1所述苦荞总黄酮制备***片的方法,其特征在于:所述步骤(2)和(3)中放置晶析的母液及步骤(1)滤出提取液Ⅲ后剩余的苦荞麸皮用于制备常规饮料及膳食纤维食品。
3.根据权利要求1所述苦荞总黄酮制备***片的方法,其特征在于:所述步骤C的木糖醇粉碎至80目以上。
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