CN104186432A - Cultivating method of nicotine-resisting black soldier fly strain - Google Patents
Cultivating method of nicotine-resisting black soldier fly strain Download PDFInfo
- Publication number
- CN104186432A CN104186432A CN201410421012.1A CN201410421012A CN104186432A CN 104186432 A CN104186432 A CN 104186432A CN 201410421012 A CN201410421012 A CN 201410421012A CN 104186432 A CN104186432 A CN 104186432A
- Authority
- CN
- China
- Prior art keywords
- nicotine
- black soldier
- soldier flies
- formulation material
- percent concentration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims abstract description 19
- 241000709785 Hermetia illucens Species 0.000 title abstract 8
- SNICXCGAKADSCV-JTQLQIEISA-N (-)-Nicotine Chemical compound CN1CCC[C@H]1C1=CC=CN=C1 SNICXCGAKADSCV-JTQLQIEISA-N 0.000 claims abstract description 172
- 229960002715 nicotine Drugs 0.000 claims abstract description 172
- SNICXCGAKADSCV-UHFFFAOYSA-N nicotine Natural products CN1CCCC1C1=CC=CN=C1 SNICXCGAKADSCV-UHFFFAOYSA-N 0.000 claims abstract description 172
- 239000000463 material Substances 0.000 claims abstract description 130
- 235000019733 Fish meal Nutrition 0.000 claims abstract description 30
- 239000004467 fishmeal Substances 0.000 claims abstract description 30
- 241001481656 Stratiomyidae Species 0.000 claims description 136
- 239000000203 mixture Substances 0.000 claims description 90
- 238000009472 formulation Methods 0.000 claims description 89
- 239000007864 aqueous solution Substances 0.000 claims description 59
- 102000002322 Egg Proteins Human genes 0.000 claims description 49
- 108010000912 Egg Proteins Proteins 0.000 claims description 49
- 210000004681 ovum Anatomy 0.000 claims description 49
- 239000002361 compost Substances 0.000 claims description 43
- 230000032683 aging Effects 0.000 claims description 29
- 235000015099 wheat brans Nutrition 0.000 claims description 29
- 238000009395 breeding Methods 0.000 claims description 18
- 238000011534 incubation Methods 0.000 claims description 10
- 238000012216 screening Methods 0.000 claims description 10
- 239000013011 aqueous formulation Substances 0.000 claims description 9
- 235000013601 eggs Nutrition 0.000 claims description 9
- 238000002360 preparation method Methods 0.000 claims description 8
- 238000005728 strengthening Methods 0.000 claims description 7
- 241000382353 Pupa Species 0.000 claims description 5
- 239000000470 constituent Substances 0.000 claims description 5
- 235000013305 food Nutrition 0.000 claims description 5
- 230000000877 morphologic effect Effects 0.000 claims description 5
- 241000287828 Gallus gallus Species 0.000 claims description 3
- 150000001875 compounds Chemical class 0.000 claims description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 17
- 241000238631 Hexapoda Species 0.000 abstract description 9
- 230000000694 effects Effects 0.000 abstract description 8
- 235000019658 bitter taste Nutrition 0.000 abstract description 2
- 235000015097 nutrients Nutrition 0.000 abstract description 2
- FFYVQVZXWDGRAY-UHFFFAOYSA-N 3-(1-methylpyrrolidin-2-yl)pyridine;hydrate Chemical compound O.CN1CCCC1C1=CC=CN=C1 FFYVQVZXWDGRAY-UHFFFAOYSA-N 0.000 abstract 2
- 230000017448 oviposition Effects 0.000 abstract 1
- 241000208125 Nicotiana Species 0.000 description 31
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 31
- 241000257159 Musca domestica Species 0.000 description 9
- 238000012360 testing method Methods 0.000 description 6
- 235000019504 cigarettes Nutrition 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 238000009825 accumulation Methods 0.000 description 4
- 230000006399 behavior Effects 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 4
- 230000007423 decrease Effects 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 3
- 239000010813 municipal solid waste Substances 0.000 description 3
- 231100000252 nontoxic Toxicity 0.000 description 3
- 230000003000 nontoxic effect Effects 0.000 description 3
- 230000000474 nursing effect Effects 0.000 description 3
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 239000004202 carbamide Substances 0.000 description 2
- 238000005520 cutting process Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 210000003608 fece Anatomy 0.000 description 2
- 230000004720 fertilization Effects 0.000 description 2
- 239000003337 fertilizer Substances 0.000 description 2
- 239000004459 forage Substances 0.000 description 2
- 239000010871 livestock manure Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000010815 organic waste Substances 0.000 description 2
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 239000002349 well water Substances 0.000 description 2
- 235000020681 well water Nutrition 0.000 description 2
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 206010042566 Superinfection Diseases 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000009418 agronomic effect Effects 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 235000013405 beer Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000013065 commercial product Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000005058 diapause Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000010794 food waste Substances 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000009571 larval growth Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 239000000618 nitrogen fertilizer Substances 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 235000010333 potassium nitrate Nutrition 0.000 description 1
- 239000004323 potassium nitrate Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000002516 radical scavenger Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000002893 slag Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 235000008939 whole milk Nutrition 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to the technical field of insect cultivating, in particular to a cultivating method of a nicotine-resisting black soldier fly strain. According to the cultivating method, black soldier fly ova of the nicotine-resisting black soldier fly strain are inoculated into first formula materials containing nicotine, the ova are cultivated until larvae are ripe and pupate, after black soldier fly pupae are subjected to emergence, a nicotine water solution is directly used for feeding adults, after the adults carry out oviposition, a next-generation domestication cultivating is carried out, and by continuous domestication cultivating of 20-30 generations, the stable nicotine-resisting black soldier fly strain can be obtained. Second formula materials are a water solution with the nicotine weight percent concentration of 0.2%-0.3%. According to the method, the nicotine water solution with the proper concentration is directly used for being combined with bran and imported fish meal in a scientific mode, the obtained first formula materials successfully cover the unpleasant smell and bitter taste of nicotine, nutrient components are proper, smooth growing of the black soldier fly larvae is guaranteed, due to the proper and certain nicotine content of the first formula materials and the second formula materials, the fact that the obtained black soldier fly ripe larva rates of the formula materials with the same weight combination in different batches under the same cultivation environment are basically the same is guaranteed, and a stable cultivating effect is obtained.
Description
Technical field
The present invention relates to insect and cultivate technical field, be specifically related to a kind of breeding method of the black soldier flies of resistance to nicotine strain.
Background technology
Inapplicable fresh tobacco leaf refers to not be suitable for the new fresh tobacco leaf (water content 80～90%) of cured tobacco production, different from the waste/hypo-tobacco leaf producing in the during production process of flue-cured tobacco of mentioning in a large number in document or discarded tobacco leaf (water content approximately 15%).Inapplicable tobacco leaf typically refers to cigarette strain and normally binds and remove after 2～3 milk pin leaves, cigarette industry is inapplicable, need remove in field bottom leaf and the upper leaf of processing, mainly comprise cigarette strain bottom because of illumination deficiency, depauperation, inclusion is not enough, estimate roasting after inferior quality, do not possess 2 bottom leaves that baking is worth, and hack at cigarette strain top, and bad, curtailment, structure are stiff, 2 tops of roasting rear inferior quality.
If these inapplicable tobacco leaves arbitrarily abandon field, very easily cause the superinfection of environmental pollution and tobacco virus, if adopt the concentrated destruction of prior art or the processing mode of landfill to need to spend a large amount of expenses, the disposal cost of industry inapplicable tobacco leaf per ton is on average up to 200 yuan at present, each tobacco leaf producing region often need to be under the organization leadership of government of local party committee, carry out policy propaganda, tabulation registration, build treatment facility, unifiedly remove, record file, publicity, cash the measures such as subsidy and supervise the processing of carrying out inapplicable tobacco leaf, the expense that wastes time and energy cost.
Adopting the insect of resistance to nicotine to carry out a biological disposal upon to inapplicable tobacco leaf is a kind of good alternate process method, environmental protection, and economic benefit is high.In order to utilize insect to realize the inapplicable fresh tobacco leaf of biological treatment, first need to filter out the insect species of resistance to nicotine.
The applicant, based on to the studying for a long period of time of housefly and inapplicable tobacco leaf treatment technology, sums up and utilizes inapplicable tobacco leaf housefly to be carried out to screening, strengthening and the breeding method of resistance to nicotine strain.Described cultivation is that the ovum of resistance to nicotine housefly is inoculated in to the first formulation material, housefly pupa sprout wings after with the second formulation material nursing adult, through the continuous domestication in 20～30 generations, obtain resistance to nicotine housefly kind.The key of its method is that the composition of the first formulation material and the second formulation material is to guarantee the smooth growth of resistance to nicotine housefly.The first formulation material consists of inapplicable fresh tobacco leaf 75～80%, wheat bran 10～15%, imported fish meal 5～10%; The second formulation material consists of whole milk powder 70%, brown sugar 29.8～29.9%, pure nicotine 0.1～0.2%.
This technical scheme can successfully be cultivated the housefly kind of resistance to nicotine, but the quantity of the aging black soldier flies larva that different batches cultivation obtains differs larger, and resistance to nicotine ability is difference to some extent also, has the uncertainty of effect.Main cause is the application aspect of inapplicable tobacco leaf, although sum up through creationary research and great many of experiments the inapplicable tobacco leaf that adds zone of reasonableness in formulation material, can guarantee the control to common house-fly grub toxic action, but the factor that affects nicotine content of tobacco leaves is not very various, complicated mechanism, more difficult realization is accurately controlled.The factor that affects nicotine content in tobacco leaf mainly comprises: (1) different growing stages, in tobacco leaf, contained nicotine content is different: in tobacco seed, nicotine content is 0, in seedling raising process, rise and start to form at root, after developing along with root system, synthetic and the accumulation ability of nicotine strengthens gradually, so different growing stages, in tobacco leaf, contained nicotine content is different; (2) cigarette strain different parts, nicotine content is significantly different, and nicotine content is also subject to leaf position and the larger impact of the number of sheets; (3) the contained nicotine amount of different cultivars tobacco leaf is different; (4) weather conditions of soil dry-wet etc. all have the impacts of different savings on nicotine content; (5) difference of agronomic measures significantly affects nicotine content of tobacco leaves, comprise fertilization type, planting density, pinch and smear wooden fork fertilising, girdling and root fertilizer, picking methods etc. the factor of cutting all significantly affects the nicotine content in tobacco leaf, take fertilization type as example, in the nitrogenous fertilizer of three kinds of forms, after urea is used, nicotine accumulation is the highest, and ammonium nitrate takes second place, the nicotine accumulation that potassium nitrate is processed is minimum, lower by 27% than using urea; Take the period of pinching as example, and Zhang Dan etc. study discovery, with postponing nicotine accumulation intensity peak the period of pinching, decline successively, and nicotine content also lowers successively, and every postponement is pinched for one day, and nicotine content just declines 0.0504%; Take again and cut root fertilizer as example, cut root 1/8 and compare with not cutting root, 1.9 percentage points of ranges of decrease 31.33% of upper leaf nicotine decline, middle leaf nicotine declines 2.13 percentage points, the range of decrease 51.4%.How avoiding the uncertainty that adopts different batches composts or fertilisers of cultivating to cultivate the aging black soldier flies larva effect obtaining is this area technical barrier urgently to be resolved hurrily.And adopt stablize feasible technical scheme cultivate the resistance to nicotine insect strain of more kinds and effectively continuous domestication make it stable, be that this area needs the important technological problems solving simultaneously.
Summary of the invention
The technical problem to be solved in the present invention is the defect that lacks the black soldier flies of resistance to nicotine kind in prior art in order to overcome, a kind of breeding method of the black soldier flies of resistance to nicotine strain is provided, the inventive method is not affected by inapplicable tobacco leaf various factors, can obtain stable cultivation effect.
Object of the present invention is achieved by the following technical programs:
Black soldier flies is a kind of insect that is different from housefly, and it is a kind of important economic insects and scavenger insect, can utilize black soldier flies to process various corrupt organic wastes, as beer slag, bean dregs, chicken manure, pig manure and changing food waste etc.Existing black soldier flies kind all has good biological treatment ability to nontoxic organic waste, but not good to the treatment effect of inapplicable fresh tobacco leaf.Main cause is because contain the toxicants such as a certain amount of nicotine in inapplicable fresh tobacco leaf, the effect that black soldier flies larva (black soldier flies mature larva) is had certain murder by poisoning and suppresses to grow.In order to utilize the black soldier flies inapplicable fresh tobacco leaf of carrying out a biological disposal upon, first need from wild black soldier flies kind, filter out the black soldier flies kind of resistance to nicotine, improve the tolerance of black soldier flies to fresh tobacco leaf.
The breeding method that the invention provides a kind of black soldier flies of resistance to nicotine strain, comprises the steps:
The ovum of the black soldier flies kind of the nicotine aqueous formulation material that is S1. 3 ‰ by tolerance mass percent concentration under indoor conditions is seeded in the first formulation material, by worm's ovum cultivate aging to larva, pupate;
S2. black soldier flies pupa utilizes the second formulation material to feed its adult after sprouting wings, and after it is laid eggs, enters next acclimatization culture from generation to generation;
S3. by above-mentioned steps, repeat, through the continuous domestication of 20～30 generations, cultivate, obtain the stable black soldier flies of resistance to nicotine strain;
Wherein, described the first formulation material is comprised of each component of following percetage by weight: wheat bran 70～90%, imported fish meal 10～30%, and with the nicotine aqueous solution of mass percent concentration 3 ‰, above-mentioned formulation material being adjusted to humidity is 70%;
The second formulation material is the aqueous solution of nicotine mass percent concentration 2 ‰～3 ‰.
Preferably, described the first formulation material is comprised of each component of following percetage by weight: wheat bran 80%, imported fish meal 20%, with the nicotine aqueous solution of mass percent concentration 3 ‰, above-mentioned formulation material being adjusted to humidity is 70%.
Preferably, the second formulation material is the aqueous solution of nicotine mass percent concentration 2.5 ‰.
Described nicotine can adopt the commercial product of purity >=99.5%.
The compound method of described the first formulation material, for after each component of the first formulation material is mixed, adds the nicotine aqueous solution of extra fine quality percent concentration that the humidity of formulation material is adjusted to 70%.
Among described every 1 kg the first formulation material, access black soldier flies ovum 0.5～1.0 g.Preferably in described every 1 kg the first formulation material, access black soldier flies ovum 0.8 g.
First the black soldier flies of the resistance to nicotine kind of the nicotine aqueous formulation material that described tolerance mass percent concentration is 3 ‰ refers to and filter out the black soldier flies of resistance to nicotine kind from wild black soldier flies kind, and then the black soldier flies of the resistance to nicotine kind screening is carried out to hot housing, make the nicotine aqueous formulation material that its tolerance mass percent concentration is 3 ‰.
The described concrete grammar that filters out the black soldier flies of resistance to nicotine kind from wild black soldier flies kind is:
S01. configuration lures collection composts or fertilisers of cultivating, will lure collection composts or fertilisers of cultivating to pack in open containers;
Described collection composts or fertilisers of cultivating each component that contains following percetage by weight that lures: wheat bran 70～90%, imported fish meal 10～30%, with the nicotine aqueous solution of mass percent concentration 1 ‰～2 ‰, above-mentioned formulation material being adjusted to humidity is 70%;
When S02. weather is fine, the morning, 8:00～9:00 lured the container of collection composts or fertilisers of cultivating to be put in respectively house refuse heap, chicken house and pig farm to lure the wild worm's ovum of collection by being equipped with, and 17:00～18:00 regained and was put in container everywhere afternoon;
S03. each container reclaiming is placed on to indoor cultivation, wait lure collection to wild worm's ovum cultivate when aging to larva, utilize the black soldier flies larva is separated with formulation material from food phase behavior of black soldier flies mature larva;
S04. by the Morphological Identification of black soldier flies larva, choose black soldier flies mature larva;
S05. filter out 20～25 days aging, every black soldier flies larvas and weigh 0.10～0.16 g, figure is plump, color is pitch-black black soldier flies mature larva, at ambient temperature, can obtain the black soldier flies kind of resistance to nicotine after allowing black soldier flies mature larva naturally pupate, to sprout wings.
Described the black soldier flies of the resistance to nicotine kind screening is carried out to hot housing, makes concrete grammar of the nicotine aqueous formulation material that its tolerance mass percent concentration is 3 ‰ be:
S11. starting stage: the ovum of the black soldier flies of resistance to nicotine kind is seeded in initial incubation material, described initial incubation material is comprised of each component of following percetage by weight: wheat bran 70～90%, imported fish meal 10～30%, and with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 70%; Under indoor conditions, by above-mentioned worm's ovum cultivate aging to larva, pupate, sprout wings after, feed its adult;
S12. the 1st strain: after the Adult worms producting eggs of S11, adopt the 1st hot housing material to cultivate cultivation on the ovum of its generation; Each component that described the 1st hot housing material contains following percetage by weight: wheat bran 70～90%, imported fish meal 10～30%, with the nicotine aqueous solution of mass percent concentration (1+S) ‰, above-mentioned formulation material being adjusted to humidity is that the value of 70%, S is selected from 0.1～0.5; Under indoor conditions, by above-mentioned worm's ovum cultivate aging to larva, pupate, sprout wings after, feed its adult;
S13. the N+1 time strain: the ovum that the adult of the N time strain gained is produced carries out hot housing again, and the N+1 time the strain contained component of composts or fertilisers of cultivating used meets following ratio: the mass percent concentration of the nicotine aqueous solution=(cultivating the content+S of nicotine in composts or fertilisers of cultivating the N time) ‰, all the other constituent contents are constant, N is more than or equal to 1 natural number, repeat strengthening, until described in last breeding phase in composts or fertilisers of cultivating the mass percent concentration of the nicotine aqueous solution reach 3 ‰.
Its adult of described nursing is all to utilize clear water to feed.Preferably, described clear water can be selected pure water, running water, well water or nontoxic river etc.; The mode that water is fed is to utilize sponge full of water, for adult, sucks.
Preferably, the indoor conditions of described cultivation black soldier flies is 25～30 ℃ of temperature, humidity 30～70%.
The invention has the beneficial effects as follows:
The present invention creatively directly adopts the nicotine aqueous solution and wheat bran and the imported fish meal science compatibility of suitable concentration, the first formulation material obtaining is not only successfully covered the unpleasant and bitter taste of nicotine, nutrient component is suitable, guarantee that black soldier flies larval growth is smooth, and because appropriateness and the certainty of the nicotine content of the first formulation material and the second formulation material, and first reasonability arranging before and after formulation material and the second formulation material nutriment and nicotine content, , guarantee in same nurturing an environment, the black soldier flies mature larva rate that the formulation material that the same weight of different batches forms obtains is basic identical, obtain stable cultivation effect.
The black soldier flies of the resistance to nicotine strain of cultivating acquisition through the present invention is owing to having passed through the long-term acclimatization culture in 20～30 generations, not only adapted to culture conditions, and formed more stable genetic character, the stability of the resistance to nicotine ability of its offspring is fine, and without the black soldier flies of the resistance to nicotine strain black soldier flies of screening and hot housing (only through) offspring that the present invention cultivates acquisition, the tolerance of nicotine is easily degenerated, stability is bad.
Embodiment
Below in conjunction with specific embodiment, further describe the present invention.Unless stated otherwise, reagent, the equipment that the present invention adopts is the conventional reagent of the art and equipment.
Nicotine is purchased from conventional chemical reagent shop, and purity is >=99.5%.
embodiment 1from wild black soldier flies kind, filter out the black soldier flies of resistance to nicotine kind
When S01. weather is fine, preparation lures collection composts or fertilisers of cultivating, described in lure collection composts or fertilisers of cultivating to be formed by each component of following percetage by weight: wheat bran 90%, imported fish meal 10%, with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 70%.
S02. by the above-mentioned composts or fertilisers of cultivating modulating, every 1 kg packs in a square plastic feed basin (the wide * of long * high=45cm*30cm*12cm) into.By the morning on the same day, 8:00 was put in the plastic basin that composts or fertilisers of cultivating is housed one life rubbish heap, afternoon, 17:00 regained plastic basin everywhere, took back indoor cultivation.
S03. (25 ℃ of temperature under indoor conditions, humidity 30%), by lure collection to wild worm's ovum cultivate to larva aging, utilize black soldier flies mature larva from food phase behavior by black soldier flies larva separated with formulation material (be that black soldier flies mature larva can be climbed from composts or fertilisers of cultivating when aging voluntarily, find dry place and pupate).
S04. under anatomical lens by the Morphological Identification of larva, with tweezers, choose separately black soldier flies mature larva, get rid of other fly kinds.
S05. filter out 20～25 days aging, every black soldier flies larvas and weigh 0.10～0.16 g, figure is plump, color is pitch-black black soldier flies mature larva, at ambient temperature, can obtain the black soldier flies kind of resistance to nicotine after allowing these black soldier flies mature larvas naturally pupate, to sprout wings.
embodiment 2from wild black soldier flies kind, filter out the black soldier flies of resistance to nicotine kind
When S01. weather is fine, preparation lures collection composts or fertilisers of cultivating, described in lure collection composts or fertilisers of cultivating to be formed by each component of following percetage by weight: wheat bran 80%, imported fish meal 20%, with the nicotine aqueous solution of mass percent concentration 1.5 ‰, above-mentioned formulation material being adjusted to humidity is 70%.
S02. by the above-mentioned composts or fertilisers of cultivating modulating, every 1kg packs in a square plastic feed basin (the wide * of long * high=45cm*30cm*12cm) into.By the morning on the same day, 8:00 was put in the plastic basin that composts or fertilisers of cultivating is housed one life rubbish heap, afternoon, 17:00 regained plastic basin everywhere, took back indoor cultivation.
S03. (25 ℃ of temperature under indoor conditions, humidity 30%), by lure collection to wild worm's ovum cultivate to larva aging, utilize black soldier flies mature larva from food phase behavior by black soldier flies larva separated with formulation material (be that black soldier flies mature larva can be climbed from composts or fertilisers of cultivating when aging voluntarily, find dry place and pupate).
S04. under anatomical lens by the Morphological Identification of larva, with tweezers, choose separately black soldier flies mature larva, get rid of other fly kinds.
S05. filter out 20～25 days aging, every black soldier flies larvas and weigh 0.10～0.16 g, figure is plump, color is pitch-black black soldier flies mature larva, at ambient temperature, can obtain the black soldier flies kind of resistance to nicotine after allowing these black soldier flies mature larvas naturally pupate, to sprout wings.
embodiment 3from wild black soldier flies kind, filter out the black soldier flies of resistance to nicotine kind
When S01. weather is fine, preparation lures collection composts or fertilisers of cultivating, described in lure collection composts or fertilisers of cultivating to be formed by each component of following percetage by weight: wheat bran 70%, imported fish meal 30%, with the nicotine aqueous solution of mass percent concentration 2 ‰, above-mentioned formulation material being adjusted to humidity is 70%.
S02. by the above-mentioned composts or fertilisers of cultivating modulating, every 1 kg packs in a square plastic feed basin (the wide * of long * high=45cm*30cm*12cm) into.By the morning on the same day, 8:00 was put in the plastic basin that composts or fertilisers of cultivating is housed one life rubbish heap, afternoon, 17:00 regained plastic basin everywhere, took back indoor cultivation.
S03. (25 ℃ of temperature under indoor conditions, humidity 30%), by lure collection to wild worm's ovum cultivate to larva aging, utilize black soldier flies mature larva from food phase behavior by black soldier flies larva separated with formulation material (be that black soldier flies mature larva can be climbed from composts or fertilisers of cultivating when aging voluntarily, find dry place and pupate).
S04. under anatomical lens by the Morphological Identification of larva, with tweezers, choose separately black soldier flies mature larva, get rid of other fly kinds.
S5. filter out 20～25 days aging, every black soldier flies larvas and weigh 0.10～0.16 g, figure is plump, color is pitch-black black soldier flies mature larva, at ambient temperature, can obtain the black soldier flies kind of resistance to nicotine after allowing these black soldier flies mature larvas naturally pupate, to sprout wings.
embodiment 4the black soldier flies of the resistance to nicotine kind screening is carried out to the formulation material that hot housing makes the nicotine aqueous solution that its tolerance mass percent concentration is 3 ‰
The black soldier flies of the resistance to nicotine kind that embodiment 1～3 any embodiment is screened is cultivated, and makes the formulation material of the nicotine aqueous solution that its tolerance mass percent concentration is 3 ‰, and concrete grammar is as follows:
S11. starting stage:
Preparation initial incubation material, described initial incubation material is comprised of each component of following percetage by weight: wheat bran 90%, imported fish meal 10%, with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 70%.By the above-mentioned composts or fertilisers of cultivating modulating, every 1 kg packs in a square plastic feed basin (the wide * of long * high=45cm*30cm*12cm), every basin access black soldier flies ovum 0.8 g into; Under indoor conditions (25～30 ℃ of temperature, humidity 30～70%), by above-mentioned worm's ovum cultivate aging to larva, pupate, sprout wings after, feed its adult.
Strain:
S12. the 1st strain: after the Adult worms producting eggs of starting stage, adopt the 1st hot housing material to carry out hot housing on the ovum of its generation; Each component that described the 1st hot housing material contains following percetage by weight: wheat bran 80%, imported fish meal 20%, with the nicotine aqueous solution of mass percent concentration (1+S) ‰, above-mentioned formulation material being adjusted to humidity is that 70%, S is selected from 0.1～0.5; Under indoor conditions (25～30 ℃ of temperature, humidity 30～70%), by above-mentioned worm's ovum cultivate aging to larva, pupate, sprout wings after, feed its adult.
S13. the N+1 time strain: the ovum that the adult of the N time strain gained is produced carries out hot housing again, and the N+1 time the strain contained component of composts or fertilisers of cultivating used meets following ratio: the mass percent concentration of the nicotine aqueous solution=(cultivating the content+S of nicotine in composts or fertilisers of cultivating the N time) ‰, all the other constituent contents are constant, N is more than or equal to 1 natural number, repeat strengthening, until described in last strain in composts or fertilisers of cultivating the mass percent concentration of the nicotine aqueous solution reach 3 ‰.
Preferably, in this enforcement, in whole breeding phase, the mass percent concentration of the nicotine aqueous solution is followed successively by 1.1 ‰, 1.2 ‰, 1.3 ‰, 1.4 ‰, 1.5 ‰, 1.6 ‰, 1.7 ‰, 1.8 ‰, 1.9 ‰, 2.0 ‰, 2.1 ‰, 2.2 ‰, 2.3 ‰, 2.4 ‰, 2.5 ‰, 2.6 ‰, 2.7 ‰, 2.8 ‰, 2.9 ‰, 3.0 ‰; Also be that black soldier flies ovum experiences 20 hot housing altogether.The black soldier flies kind that this stage turns out has higher tolerance to nicotine.
embodiment 5the black soldier flies of the resistance to nicotine kind screening is carried out to the formulation material that hot housing makes the nicotine aqueous solution that its tolerance mass percent concentration is 3 ‰
The black soldier flies of the resistance to nicotine kind that embodiment 1～3 any embodiment is screened is cultivated, and makes the formulation material of the nicotine aqueous solution that its tolerance mass percent concentration is 3 ‰, and concrete grammar is as follows:
S11. starting stage:
Preparation initial incubation material, described initial incubation material is comprised of each component of following percetage by weight: wheat bran 80%, imported fish meal 20%, with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 70%.By the above-mentioned composts or fertilisers of cultivating modulating, every 1 kg packs in a square plastic feed basin (the wide * of long * high=45cm*30cm*12cm), every basin access black soldier flies ovum 0.8 g into; Under indoor conditions (25～30 ℃ of temperature, humidity 30～70%), by above-mentioned worm's ovum cultivate aging to larva, pupate, sprout wings after, feed its adult.
Strain:
S12. the 1st strain: after the Adult worms producting eggs of starting stage, adopt the 1st hot housing material to carry out hot housing on the ovum of its generation; Each component that described the 1st hot housing material contains following percetage by weight: wheat bran 80%, imported fish meal 20%, with the nicotine aqueous solution of mass percent concentration (1+S) ‰, above-mentioned formulation material being adjusted to humidity is that 70%, S is selected from 0.1～0.5; Under indoor conditions (25～30 ℃ of temperature, humidity 30～70%), by above-mentioned worm's ovum cultivate aging to larva, pupate, sprout wings after, feed its adult;
S13. the N+1 time strain: the ovum that the adult of the N time strain gained is produced carries out hot housing again, and the N+1 time the strain contained component of composts or fertilisers of cultivating used meets following ratio: the mass percent concentration of the nicotine aqueous solution=(cultivating the content+S of nicotine in composts or fertilisers of cultivating the N time) ‰, all the other constituent contents are constant, N is more than or equal to 1 natural number, repeat strengthening, until described in last strain in composts or fertilisers of cultivating the mass percent concentration of the nicotine aqueous solution reach 3 ‰.
Preferably, in this enforcement, in whole breeding phase, the mass percent concentration of the nicotine aqueous solution is followed successively by 1.2 ‰, 1.4 ‰, 1.6 ‰, 1.8 ‰, 2.0 ‰, 2.2 ‰, 2.4 ‰, 2.6 ‰, 2.8 ‰, 3.0 ‰; Also be that black soldier flies ovum experiences 10 hot housing altogether.The black soldier flies kind that this stage turns out has higher tolerance to nicotine.
embodiment 6the black soldier flies of the resistance to nicotine kind screening is carried out to the formulation material that hot housing makes the nicotine aqueous solution that its tolerance mass percent concentration is 3 ‰
The black soldier flies of the resistance to nicotine kind that embodiment 1～3 any embodiment is screened is cultivated, and makes the formulation material of the nicotine aqueous solution that its tolerance mass percent concentration is 3 ‰, and concrete grammar is as follows:
S11. starting stage:
Preparation initial incubation material, described initial incubation material is comprised of each component of following percetage by weight: wheat bran 70%, imported fish meal 30%, with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 70%.By the above-mentioned composts or fertilisers of cultivating modulating, every 1 kg packs in a square plastic feed basin (the wide * of long * high=45cm*30cm*12cm), every basin access black soldier flies ovum 0.8 g into; Under indoor conditions (25～30 ℃ of temperature, humidity 30～70%), by above-mentioned worm's ovum cultivate aging to larva, pupate, sprout wings after, feed its adult.Its adult of described nursing is all to utilize clear water to feed.Described clear water can be selected pure water, running water, well water or nontoxic river etc.; The mode that water is fed is to utilize sponge full of water, for adult, sucks.Lower same.
Strain:
S12. the 1st strain: after the Adult worms producting eggs of starting stage, adopt the 1st hot housing material to carry out hot housing on the ovum of its generation; Each component that described the 1st hot housing material contains following percetage by weight: wheat bran 80%, imported fish meal 20%, with the nicotine aqueous solution of mass percent concentration (1+S) ‰, above-mentioned formulation material being adjusted to humidity is that 70%, S is selected from 0.1～0.5; Under indoor conditions (25～30 ℃ of temperature, humidity 30～70%), by above-mentioned worm's ovum cultivate aging to larva, pupate, sprout wings after, feed its adult;
S13. the N+1 time strain: the ovum that the adult of the N time strain gained is produced carries out hot housing again, and the N+1 time the strain contained component of composts or fertilisers of cultivating used meets following ratio: the mass percent concentration of the nicotine aqueous solution=(cultivating the content+S of nicotine in composts or fertilisers of cultivating the N time) ‰, all the other constituent contents are constant, N is more than or equal to 1 natural number, repeat strengthening, until described in last strain in composts or fertilisers of cultivating the mass percent concentration of the nicotine aqueous solution reach 3 ‰.
Preferably, in this enforcement, in whole breeding phase, the mass percent concentration of the nicotine aqueous solution is followed successively by 1.5 ‰, 2.0 ‰, 2.5 ‰, 3.0 ‰; Also be that black soldier flies ovum experiences 4 hot housing altogether.The black soldier flies kind that this stage turns out has higher tolerance to nicotine.
embodiment 7the continuous domestication of a plurality of generations is cultivated and is obtained the stable black soldier flies of resistance to nicotine strain
The black soldier flies kind of the nicotine aqueous formulation material that the tolerance mass percent concentration that embodiment 4～6 any embodiment are cultivated is 3 ‰ is cultivated through the continuous domestication of a plurality of generations again, forms the stable black soldier flies of resistance to nicotine strain.Concrete grammar is:
S1. under indoor conditions, the ovum of the black soldier flies kind of the nicotine aqueous formulation material that is 3 ‰ by tolerance mass percent concentration is seeded in the first formulation material, by worm's ovum cultivate aging to larva, pupate;
S2. black soldier flies pupa utilizes the second formulation material to feed its adult after sprouting wings, and after it is laid eggs, enters next acclimatization culture from generation to generation;
S3. by above-mentioned steps, repeat, through the continuous domestication of 30 generations, cultivate, obtain the stable black soldier flies of resistance to nicotine strain;
The preparation of the first formulation material: described the first formulation material is comprised of each component of following percetage by weight: wheat bran 90%, imported fish meal 10%, with the nicotine aqueous solution of mass percent concentration 3 ‰, above-mentioned formulation material being adjusted to humidity is 70%.
By the above-mentioned formulation material modulating, every 1 kg packs in a square plastic feed basin (the wide * of long * high=45cm*30cm*12cm), every basin access black soldier flies ovum 0.8 g into;
Under indoor conditions (25～30 ℃ of temperature, humidity 30～70%), by above-mentioned worm's ovum cultivate aging to larva, pupate;
Black soldier flies pupa utilizes the second formulation material to feed its adult after sprouting wings, and the second formulation material is the aqueous solution of nicotine mass percent concentration 2 ‰, after it is laid eggs, enters next acclimatization culture from generation to generation;
By above-mentioned steps, repeat, through the continuous domestication of 30 generations, cultivate, obtain the stable black soldier flies of resistance to nicotine strain.
embodiment 8
Other operations are with embodiment 7, and different is described the first formulation material is comprised of each component of following percetage by weight: wheat bran 80%, imported fish meal 20%, with the nicotine aqueous solution of mass percent concentration 3 ‰, above-mentioned formulation material being adjusted to humidity is 70%.Described the second formulation material is the aqueous solution of nicotine mass percent concentration 2.5 ‰.Continuous domestication through 25 generations is cultivated, and obtains the stable black soldier flies of resistance to nicotine strain.
embodiment 9
Other operations are with embodiment 7, and different is described the first formulation material is comprised of each component of following percetage by weight: wheat bran 70%, imported fish meal 30%, with the nicotine aqueous solution of mass percent concentration 3 ‰, above-mentioned formulation material being adjusted to humidity is 70%.Described the second formulation material is the aqueous solution of nicotine mass percent concentration 3 ‰.Continuous domestication through 20 generations is cultivated, and obtains the stable black soldier flies of resistance to nicotine strain.
comparative example 1
Operation is with embodiment 1, lures collection composts or fertilisers of cultivating to be comprised of each component of following percetage by weight: wheat bran 80% and imported fish meal 20% described in different is.Experiment find to utilize as above lure collection composts or fertilisers of cultivating lure collection to ovum be not the kind of resistance to nicotine.
Embodiment 10
The black soldier flies kind that the embodiment of the present invention 1 to 3 is screened, embodiment 4 to 6 carry out resistance to nicotine aptitude tests through the black soldier flies kind of cultivating after black soldier flies kind, embodiment 7 to 9 black soldier flies kind, each comparative example through too much cultivating for continuous domestication of the nicotine formulation material of tolerance 3 ‰ processed, and test process is:
Get respectively described embodiment and cultivate the ovum 0.5g that the black soldier flies adult after processing produces, access respectively formulation material (wheat bran 80%, imported fish meal 20% containing the better quality percent concentration nicotine aqueous solution that 1kg is heavy, with the nicotine aqueous solution of mass percent concentration 3.0 ‰, above-mentioned formulation material being adjusted to humidity is 70%) in, test result is shown in Table 1:
Table 1
Test result explanation, comparative example 1 can not tolerate the nicotine aqueous solution of mass percent concentration 3.0 ‰.
There is diapause phenomenon in 1 to 3 black soldier flies kind through screening of embodiment, larva body weight is lighter, and has relatively large death in the formulation material of the nicotine aqueous solution modulation of mass percent concentration 3.0 ‰.The resistance to nicotine ability of the black soldier flies kind after embodiment 4 to 9 hot housing are processed improves greatly, can tolerate the formulation material of the nicotine aqueous solution modulation of mass percent concentration 3.0 ‰.
Embodiment 11 stability experiments
The black soldier flies of the resistance to nicotine kind obtaining through method described in embodiment 4 to 6 is carried out to routine cultivation, and (wherein black soldier flies larva culture material formula is wheat bran 80%+ fish meal 20%, with clear water, above-mentioned formulation material being adjusted to humidity is 70%, and adult forage is clear water) standby after 25 generations.Simultaneously, the black soldier flies of the resistance to nicotine strain utilization obtaining through method described in embodiment 7 to 9 is cultivated to material, and (wherein black soldier flies larva culture material formula is wheat bran 80%, imported fish meal 20%, with the nicotine aqueous solution of mass percent concentration 3.0 ‰, above-mentioned formulation material being adjusted to humidity is 70%, and adult forage is the nicotine aqueous solution of mass percent concentration 2.5 ‰) continue again to cultivate after 25 generations standby.The black soldier flies kind of above-mentioned two strains is carried out to resistance to nicotine aptitude tests, detecting is wheat bran 80%, imported fish meal 20% with culture material formula, by the humidity that the nicotine aqueous solution of mass percent concentration 3 ‰ is adjusted to formulation material by above-mentioned formulation material, be 70%, whether the resistance to nicotine ability that detects its offspring declines.Test result is shown in Table 2:
The stability experiment result of the black soldier flies strain of table 2 after 25 generations
| Process | Larvae development go through the phase (my god) | Larva number/g(bar) | Survival rate of larvae (%) |
| The conventional strain of embodiment 4 | 7 | 48 | 63 |
| The conventional strain of embodiment 5 | 7 | 47 | 66 |
| The conventional strain of embodiment 6 | 7 | 48 | 64 |
| Embodiment 7 cultivates strain | 5 | 28 | 96 |
| Embodiment 8 cultivates strain | 5 | 28 | 96 |
| Embodiment 9 cultivates strain | 5 | 29 | 95 |
Result show through strengthening after the black soldier flies of resistance to nicotine kind after cultivating for 25 generations with cellar culture material, there is serious degradation phenomenon in resistance to nicotine ability, and the black soldier flies of the resistance to nicotine strain offspring who cultivates acquisition through the present invention is fine to the stability of nicotine tolerance, and in formulation material compositing range of the present invention, the black soldier flies that the formulation material that the same weight of different batches forms obtains has stable cultivation effect.
Claims (9)
1. a breeding method for the black soldier flies of resistance to nicotine strain, is characterized in that, comprises the steps:
S1. under indoor conditions, the ovum of the black soldier flies kind of the nicotine aqueous formulation material that is 3 ‰ by tolerance mass percent concentration is seeded in the first formulation material, by worm's ovum cultivate aging to larva, pupate;
S2. black soldier flies pupa utilizes the second formulation material to feed its adult after sprouting wings, and after it is laid eggs, enters next acclimatization culture from generation to generation;
S3. by above-mentioned steps, repeat, through the continuous domestication of 20～30 generations, cultivate, obtain the stable black soldier flies of resistance to nicotine strain;
Wherein, described the first formulation material is comprised of each component of following percetage by weight: wheat bran 70～90%, imported fish meal 10～30%, and with the nicotine aqueous solution of mass percent concentration 3 ‰, above-mentioned formulation material being adjusted to humidity is 70%;
The second formulation material is the aqueous solution of nicotine mass percent concentration 2 ‰～3 ‰.
2. breeding method according to claim 1, is characterized in that, indoor conditions described in S1 is 25～30 ℃ of temperature, humidity 30～70%.
3. breeding method according to claim 1, it is characterized in that, described the first formulation material is comprised of each component of following percetage by weight: wheat bran 80%, imported fish meal 20%, with the nicotine aqueous solution of mass percent concentration 3 ‰, above-mentioned formulation material being adjusted to humidity is 70%.
4. breeding method according to claim 1, is characterized in that, described the second formulation material is the aqueous solution of nicotine mass percent concentration 2.5 ‰.
5. breeding method according to claim 1, is characterized in that, the compound method of described the first formulation material is for after mixing each component of the first formulation material, and with the nicotine aqueous solution of mass percent concentration 3 ‰, above-mentioned formulation material being adjusted to humidity is 70%.
6. breeding method according to claim 1, is characterized in that, among described every 1 kg the first formulation material, accesses black soldier flies ovum 0.5～1.0g.
7. breeding method according to claim 1, it is characterized in that, the preparation method of the black soldier flies of the resistance to nicotine kind of the nicotine aqueous formulation material that described tolerance mass percent concentration is 3 ‰ is: first from wild black soldier flies kind, filter out the black soldier flies of resistance to nicotine kind, and then the black soldier flies of the resistance to nicotine kind screening is carried out to hot housing, make the nicotine aqueous formulation material that its tolerance mass percent concentration is 3 ‰.
8. breeding method according to claim 7, is characterized in that, the method for the screening of the described black soldier flies of resistance to nicotine kind is:
S1. configuration lures collection composts or fertilisers of cultivating, will lure collection composts or fertilisers of cultivating to pack in open containers;
Described collection composts or fertilisers of cultivating each component that contains following percetage by weight that lures: wheat bran 70～90%, imported fish meal 10～30%, with the nicotine aqueous solution of mass percent concentration 1 ‰～2 ‰, above-mentioned formulation material being adjusted to humidity is 70%;
When S2. weather is fine, lure the container of collection composts or fertilisers of cultivating to be put in respectively house refuse heap, chicken house and pig farm to lure the wild black soldier flies worm's ovum of collection, regain afternoon and be put in container everywhere the morning by being equipped with;
S3. each container reclaiming is placed on to indoor cultivation, wait lure collection to wild worm's ovum cultivate when aging to larva, utilize the black soldier flies larva is separated with formulation material from food phase behavior of black soldier flies mature larva;
S4. by the Morphological Identification of black soldier flies larva, choose black soldier flies mature larva;
S5. filter out 20～25 days aging, every black soldier flies larvas and weigh 0.10～0.16g, figure is plump, color is pitch-black black soldier flies mature larva, at ambient temperature, can obtain the black soldier flies kind of resistance to nicotine after allowing black soldier flies mature larva naturally pupate, to sprout wings.
9. breeding method according to claim 7, is characterized in that, the method for the hot housing of the described black soldier flies of resistance to nicotine kind is:
Starting stage: the ovum of the black soldier flies of resistance to nicotine kind is seeded in initial incubation material, described initial incubation material is comprised of each component of following percetage by weight: wheat bran 70～90%, imported fish meal 10～30%, and with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 70%; Under indoor conditions, by above-mentioned worm's ovum cultivate aging to larva, pupate, sprout wings after, feed adult;
The 1st strain: after the Adult worms producting eggs of starting stage, adopt the 1st hot housing material to carry out hot housing on the ovum of its generation; Described the 1st hot housing material is comprised of each component of following percetage by weight: wheat bran 70～90%, imported fish meal 10～30%, with the nicotine aqueous solution of mass percent concentration (1+S) ‰, above-mentioned formulation material being adjusted to humidity is that 70%, S is selected from 0.1～0.5; Under indoor conditions, by above-mentioned worm's ovum cultivate aging to larva, pupate, sprout wings after, feed adult;
The N+1 time strain: the ovum that the adult of the N time strain gained is produced carries out hot housing again, and the N+1 time the strain contained component of composts or fertilisers of cultivating used meets following ratio: the mass percent concentration of the nicotine aqueous solution=(content+S of nicotine in the N time hot housing material) ‰, all the other constituent contents are constant, N is more than or equal to 1 natural number, repeat strengthening, until described in last strain in composts or fertilisers of cultivating the mass percent concentration of the nicotine aqueous solution reach 3 ‰.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410421012.1A CN104186432B (en) | 2014-08-25 | 2014-08-25 | Cultivating method of nicotine-resisting black soldier fly strain |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410421012.1A CN104186432B (en) | 2014-08-25 | 2014-08-25 | Cultivating method of nicotine-resisting black soldier fly strain |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104186432A true CN104186432A (en) | 2014-12-10 |
| CN104186432B CN104186432B (en) | 2017-05-17 |
Family
ID=52071952
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410421012.1A Expired - Fee Related CN104186432B (en) | 2014-08-25 | 2014-08-25 | Cultivating method of nicotine-resisting black soldier fly strain |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104186432B (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101889629A (en) * | 2010-06-13 | 2010-11-24 | 中山大学 | Method for processing food waste by using black soldier fly larvae and material formula |
| CN103283681A (en) * | 2012-03-05 | 2013-09-11 | 朱大恒 | Biological utilization method of waste tobacco stems |
| CN103563858A (en) * | 2013-11-07 | 2014-02-12 | 南雄市中大赛尔生物技术有限公司 | Reinforcing method for nicotine-resistant housefly species |
| CN103563857A (en) * | 2013-11-07 | 2014-02-12 | 中国烟草总公司广东省公司 | Screening method for nicotine-resistant housefly species |
| CN103651263A (en) * | 2013-11-07 | 2014-03-26 | 中山大学 | Cultivation method of nicotine-resistant housefly strain |
| CN103975902A (en) * | 2014-05-30 | 2014-08-13 | 中山大学 | Method for attracting wild hermetia illucens |
-
2014
- 2014-08-25 CN CN201410421012.1A patent/CN104186432B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101889629A (en) * | 2010-06-13 | 2010-11-24 | 中山大学 | Method for processing food waste by using black soldier fly larvae and material formula |
| CN103283681A (en) * | 2012-03-05 | 2013-09-11 | 朱大恒 | Biological utilization method of waste tobacco stems |
| CN103563858A (en) * | 2013-11-07 | 2014-02-12 | 南雄市中大赛尔生物技术有限公司 | Reinforcing method for nicotine-resistant housefly species |
| CN103563857A (en) * | 2013-11-07 | 2014-02-12 | 中国烟草总公司广东省公司 | Screening method for nicotine-resistant housefly species |
| CN103651263A (en) * | 2013-11-07 | 2014-03-26 | 中山大学 | Cultivation method of nicotine-resistant housefly strain |
| CN103975902A (en) * | 2014-05-30 | 2014-08-13 | 中山大学 | Method for attracting wild hermetia illucens |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104186432B (en) | 2017-05-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103651263B (en) | A kind of breeding method of resistance to nicotine housefly strain | |
| CN103563857B (en) | A kind of screening technique of resistance to nicotine housefly kind | |
| CN104798734B (en) | Method for feeding predatory coenosia larvae indoors | |
| CN103563858A (en) | Reinforcing method for nicotine-resistant housefly species | |
| CN104187110A (en) | Formula material for trapping wild black soldier flies to lay eggs and screening method of nicotine-resistant black soldier fly varieties | |
| Ashrafi et al. | Reuse of spent mushroom substrate as casing material for the production of milky white mushroom. | |
| Maurya et al. | Impact of different substrates for spawn production and production of milky mushroom (Calocybe indica) | |
| Das et al. | Use of wild grasses as substrate for cultivation of oyster mushroom in South West Bengal | |
| CN104186434A (en) | Cultivating method of nicotine-resisting chrysomyia megacephala strain | |
| CN104285894B (en) | Strengthening method of nicotine-resistant boettcherisca peregrina variety | |
| CN104285892B (en) | A kind of breeding method of resistance to nicotine Sarcophga fuscicauda strain | |
| CN104285891B (en) | A kind of breeding method of the lucilia sericata of resistance to nicotine strain | |
| CN104186433B (en) | A kind of breeding method of the black of resistance to nicotine phalangeal process stratiomyiid strain | |
| Alam | Study on the Cultural Suitability of Amblypharyngodon mola with Barbodes gonionotus and Cyprinus carpio in a Farmer's Rice Fields MJ Alam, S. Dewan, MR Rahman, M. Kunda," MA Khaleque and" MA Kader | |
| CN104186432A (en) | Cultivating method of nicotine-resisting black soldier fly strain | |
| CN104285897B (en) | A kind of intensifying method of the black soldier flies of resistance to nicotine kind | |
| CN104285895B (en) | A kind of intensifying method of the lucilia sericata of resistance to nicotine kind | |
| CN104187107A (en) | Formula material for trapping wild boettcherisca peregrina to lay eggs and screening method of nicotine-resistant boettcherisca peregrina varieties | |
| CN104187109A (en) | Formula material for trapping wild chrysomyia megacephala to lay eggs and screening method of nicotine-resistant chrysomyia megacephala varieties | |
| CN104187108A (en) | Formula material for trapping wild ptecticus tenebrifer to lay eggs and screening method of nicotine-resistant ptecticus tenebrifer varieties | |
| CN104285896B (en) | Method for breeding nicotine-resisting ptecticus aurifer strains | |
| CN104285898B (en) | A kind of black of resistance to nicotine refers to the intensifying method of gushing water horsefly kind | |
| CN104285899B (en) | Method for strengthening nicotine-resisting chrysomyia megacephala breeds | |
| CN104285893B (en) | A kind of intensifying method of resistance to nicotine golden yellow phalangeal process stratiomyiid kind | |
| CN104304693B (en) | Trap the formulation material of wild lucilia sericata spawning and the screening technique of the lucilia sericata of resistance to nicotine kind |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170517 Termination date: 20190825 |