CN104285896B - Method for breeding nicotine-resisting ptecticus aurifer strains - Google Patents
Method for breeding nicotine-resisting ptecticus aurifer strains Download PDFInfo
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- CN104285896B CN104285896B CN201410421786.4A CN201410421786A CN104285896B CN 104285896 B CN104285896 B CN 104285896B CN 201410421786 A CN201410421786 A CN 201410421786A CN 104285896 B CN104285896 B CN 104285896B
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- nicotine
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- water horsefly
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- 238000000034 method Methods 0.000 title claims abstract description 24
- 238000009395 breeding Methods 0.000 title claims abstract description 20
- 230000001488 breeding effect Effects 0.000 title abstract description 8
- 241001189097 Ptecticus aurifer Species 0.000 title abstract 6
- SNICXCGAKADSCV-JTQLQIEISA-N (-)-Nicotine Chemical compound CN1CCC[C@H]1C1=CC=CN=C1 SNICXCGAKADSCV-JTQLQIEISA-N 0.000 claims abstract description 169
- 229960002715 nicotine Drugs 0.000 claims abstract description 169
- SNICXCGAKADSCV-UHFFFAOYSA-N nicotine Natural products CN1CCCC1C1=CC=CN=C1 SNICXCGAKADSCV-UHFFFAOYSA-N 0.000 claims abstract description 169
- 239000000463 material Substances 0.000 claims abstract description 118
- 239000007864 aqueous solution Substances 0.000 claims abstract description 62
- 230000032683 aging Effects 0.000 claims abstract description 33
- 235000019733 Fish meal Nutrition 0.000 claims abstract description 22
- 239000004467 fishmeal Substances 0.000 claims abstract description 22
- 241000382353 Pupa Species 0.000 claims abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 142
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 claims description 131
- 241000255632 Tabanus atratus Species 0.000 claims description 126
- 239000000203 mixture Substances 0.000 claims description 86
- 238000009472 formulation Methods 0.000 claims description 81
- 102000002322 Egg Proteins Human genes 0.000 claims description 49
- 108010000912 Egg Proteins Proteins 0.000 claims description 49
- 210000004681 ovum Anatomy 0.000 claims description 49
- 239000002361 compost Substances 0.000 claims description 46
- 235000015099 wheat brans Nutrition 0.000 claims description 28
- 238000011534 incubation Methods 0.000 claims description 11
- 238000012216 screening Methods 0.000 claims description 9
- 239000013011 aqueous formulation Substances 0.000 claims description 7
- 238000005728 strengthening Methods 0.000 claims description 6
- 239000000470 constituent Substances 0.000 claims description 5
- 235000013601 eggs Nutrition 0.000 claims description 5
- 235000013305 food Nutrition 0.000 claims description 5
- 230000000877 morphologic effect Effects 0.000 claims description 5
- 230000003252 repetitive effect Effects 0.000 claims description 4
- 238000012549 training Methods 0.000 claims description 4
- 241000287828 Gallus gallus Species 0.000 claims description 3
- 150000001875 compounds Chemical class 0.000 claims description 2
- 238000002360 preparation method Methods 0.000 claims description 2
- FFYVQVZXWDGRAY-UHFFFAOYSA-N 3-(1-methylpyrrolidin-2-yl)pyridine;hydrate Chemical compound O.CN1CCCC1C1=CC=CN=C1 FFYVQVZXWDGRAY-UHFFFAOYSA-N 0.000 claims 1
- 241000255626 Tabanus <genus> Species 0.000 claims 1
- 239000000243 solution Substances 0.000 claims 1
- 235000019658 bitter taste Nutrition 0.000 abstract description 2
- 230000012010 growth Effects 0.000 abstract description 2
- 235000016709 nutrition Nutrition 0.000 abstract description 2
- 239000004615 ingredient Substances 0.000 abstract 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 31
- 241000208125 Nicotiana Species 0.000 description 16
- 244000061176 Nicotiana tabacum Species 0.000 description 15
- 230000000694 effects Effects 0.000 description 8
- 241000257159 Musca domestica Species 0.000 description 7
- 230000008569 process Effects 0.000 description 7
- 235000019504 cigarettes Nutrition 0.000 description 5
- 239000002917 insecticide Substances 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 4
- 238000009825 accumulation Methods 0.000 description 4
- 230000006399 behavior Effects 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 235000013360 fish flour Nutrition 0.000 description 4
- 239000000843 powder Substances 0.000 description 4
- 241000251468 Actinopterygii Species 0.000 description 3
- 241000238631 Hexapoda Species 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 3
- 230000007423 decrease Effects 0.000 description 3
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 3
- 239000010931 gold Substances 0.000 description 3
- 229910052737 gold Inorganic materials 0.000 description 3
- 230000008676 import Effects 0.000 description 3
- 239000010813 municipal solid waste Substances 0.000 description 3
- 231100000252 nontoxic Toxicity 0.000 description 3
- 230000003000 nontoxic effect Effects 0.000 description 3
- 235000013877 carbamide Nutrition 0.000 description 2
- 239000004202 carbamide Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 210000003608 fece Anatomy 0.000 description 2
- 230000004720 fertilization Effects 0.000 description 2
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- 230000000474 nursing effect Effects 0.000 description 2
- 239000010815 organic waste Substances 0.000 description 2
- 230000000704 physical effect Effects 0.000 description 2
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
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- 239000002699 waste material Substances 0.000 description 2
- 239000002349 well water Substances 0.000 description 2
- 235000020681 well water Nutrition 0.000 description 2
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 description 1
- 206010058314 Dysplasia Diseases 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000009418 agronomic effect Effects 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
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- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
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- 238000005520 cutting process Methods 0.000 description 1
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- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000010794 food waste Substances 0.000 description 1
- 239000004459 forage Substances 0.000 description 1
- WXSRWSCQVXYHSN-UHFFFAOYSA-N gold;3-(1-methylpyrrolidin-2-yl)pyridine Chemical compound [Au].CN1CCCC1C1=CC=CN=C1 WXSRWSCQVXYHSN-UHFFFAOYSA-N 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 230000001418 larval effect Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000618 nitrogen fertilizer Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 235000010333 potassium nitrate Nutrition 0.000 description 1
- 239000004323 potassium nitrate Substances 0.000 description 1
- 239000002516 radical scavenger Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 235000008939 whole milk Nutrition 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Life Sciences & Earth Sciences (AREA)
- Polymers & Plastics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Food Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Environmental Sciences (AREA)
- Biotechnology (AREA)
- Mycology (AREA)
- Botany (AREA)
- Animal Behavior & Ethology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Insects & Arthropods (AREA)
- Birds (AREA)
- Biomedical Technology (AREA)
- Fodder In General (AREA)
- Mushroom Cultivation (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a method for breeding nicotine-resisting ptecticus aurifer strains. The breeding method comprises the steps that nicotine-resisting ptecticus aurifer spawns are inoculated in first formula materials with nicotine, the spawns are bred to larvae to be aged and pupated, adults are fed directly through the nicotine aqueous solution after pupa emergence, after the adults spawn, the next generation is domesticated and bred, and the stable nicotine-resisting ptecticus aurifer strains can be obtained after continuous domestication and breeding of 20-30 generations. The aqueous solution with the nicotine mass percent concentration of two permillage to three permillage is adopted as second formula materials. The nicotine aqueous solution with the proper concentration is directly and scientifically combined with bran and imported fish meal, the obtained formula materials successfully cover the bad smell and bitter taste of the nicotine, nutritional ingredients are proper, smooth growth of the ptecticus aurifer larvae is guaranteed, the aging larva rates of the ptecticus aurifer obtained through the formula materials composed of the same weight of different batches are basically identical, and the stable breeding effect is obtained.
Description
Technical field
The present invention relates to insecticide Cultivating techniques field and in particular to a kind of resistance to nicotine golden yellow refer to gushing water horsefly strain cultivation side
Method.
Background technology
Inapplicable fresh tobacco leaf refers to not be suitable for the fresh tobacco leaves (water content 80～90%) of cured tobacco production, big with document
Measure the waste/hypo-tobacco leaf producing in the production process of flue-cured tobacco referring to or discarded tobacco leaf (water content about 15%) is different.Inapplicable Nicotiana tabacum L. leads to
Refer to after cigarette strain normally binds and remove 2～3 milk foot leaves, cigarette industry is inapplicable, need to be under field is removed and processed
Portion's leaf and upper leaf, main include cigarette strain bottom because illumination deficiency, dysplasia, inclusions are not enough, estimated roasting rear inferior quality,
Do not possess 2 inferior leads that baking is worth, and the hacking of cigarette strain top bad, curtailment, structure are stiff, roasting after inferior quality
2 tops.
If these inapplicable Nicotiana tabacum Lves arbitrarily abandon field, easily cause environmental pollution and tobacco viruses repeat feel
Dye, the processing mode destroyed or fill of concentrating according to prior art then needs to spend substantial amounts of expense, and current industry is per ton not
It is suitable for averagely up to 200 yuan of the processing cost of Nicotiana tabacum L., each Nicotiana tabacum L. producing region generally requires under the organization leadership of government of local party committee,
Carry out policy propaganda, tabulation registration, build treatment facility, unified remove, record archive, publicity, cash the measures such as subsidy and supervise
Carry out the process of inapplicable Nicotiana tabacum L., waste time and energy costly.
Using the insecticide of resistance to nicotine, inapplicable Nicotiana tabacum L. being carried out with biological treatment is a kind of preferable alternate process method, ring
Protect, high financial profit.In order to realize, using insecticide, inapplicable fresh tobacco leaf of carrying out a biological disposal upon, need to filter out the insecticide product of resistance to nicotine first
Kind.
The applicant studies for a long period of time based on to housefly and inapplicable Nicotiana tabacum L. treatment technology, sums up using inapplicable Nicotiana tabacum L.
Housefly is carried out with screening, strengthening and the breeding method of resistance to nicotine strain.Described cultivation is that the ovum of resistance to nicotine housefly is inoculated in
One formulation material, family's fly pupa feeds adult with the second formulation material after sprouting wings, and the continuous domestication through 20～30 generations obtains resistance to nicotine man
Fly kind.The smooth life forming to ensure resistance to nicotine housefly that it is critical only that the first formulation material and the second formulation material of its method
Long.First formulation material consists of inapplicable fresh tobacco leaf 75～80%, wheat bran 10～15%, imported fish meal 5～10%；Second formulation material
Consist of whole milk powder 70%, brown sugar 29.8～29.9%, pure nicotine 0.1～0.2%.
This technical scheme can go out the housefly kind of resistance to nicotine with successful incubation, but different batches cultivate the aging gold obtaining
The quantity difference that Huang refers to gushing water horsefly larva is larger, and resistance to nicotine ability is also otherwise varied, there is the uncertainty of effect.Main cause
It is the application aspect of inapplicable Nicotiana tabacum L. and add rationally although summing up in formulation material through creative research and many experiments
The inapplicable Nicotiana tabacum L. of scope can ensure the control to Musca domestica larva toxic action, but affects the factor of not nicotine content of tobacco leaves very
Various, complicated mechanism is it is more difficult to realize accurately controlling.The factor of impact nicotine content in tobacco leaf specifically includes that (1) different growth
Phase, in Nicotiana tabacum L., contained nicotine content is different: in tobacco seed, nicotine content is 0, plays beginning and formed in root in seedling raising process, after
Developing with root system, the synthesis of nicotine and accumulation ability gradually strengthen, so different growing stages, contained nicotine in Nicotiana tabacum L.
Content is different；(2) cigarette strain different parts, nicotine content is dramatically different, and nicotine content is also subject to leaf position and number of sheets large effect；
(3) nicotine amount contained by different cultivars Nicotiana tabacum L. is different；(4) weather conditions of soil dry-wet etc. all have different savings to nicotine content
Impact；(5) the different appreciable impact nicotine content of tobacco leaves of agronomic measures, including fertilization type, planting density, pinch and smear wooden fork and apply
Fertile, girdling and cut nicotine content in root fertilizer, picking methods etc. factor all appreciable impact Nicotiana tabacum Lves, taking fertilization type as a example,
In the nitrogenous fertilizer of three kinds of forms, after carbamide is applied, nicotine accumulation highest, ammonium nitrate takes second place, and the nicotine accumulation that potassium nitrate is processed is minimum, than
Apply carbamide low by 27%；Taking Physical property as a example, Zhang Dan etc. research find, with Physical property postpone nicotine accumulation intensity peak according to
Secondary decline, nicotine content also lowers successively, often postpones and pinching for one day, nicotine content just declines 0.0504%；To cut root fertilizer it is again
Example, cuts root 1/8 compared with not cutting root, and upper leaf nicotine declines 1.9 percentage points of ranges of decrease 31.33%, and middle leaf nicotine declines 2.13
Percentage point, the range of decrease 51.4%.How to avoid cultivating the aging golden yellow finger gushing water horsefly larva effect obtaining using different batches compost
The uncertainty of fruit is this area technical barrier urgently to be resolved hurrily.And cultivate more multi items using stablizing feasible technical scheme
The insect strain of resistance to nicotine and effectively continuous domestication stabilize it, be this area simultaneously need to the important technological problems that solve.
Content of the invention
The technical problem to be solved in the present invention is to lack resistance to nicotine golden yellow finger gushing water horsefly kind in prior art to overcome
Defect, provide a kind of resistance to nicotine golden yellow refer to gushing water horsefly strain breeding method, the inventive method be not subject to inapplicable Nicotiana tabacum L. various because
Element impact, can obtain stable cultivate effect.
The purpose of the present invention is achieved by the following technical programs:
Golden yellow finger gushing water horsefly is a kind of insecticide different from housefly, and it is a kind of important economic insects and scavenger insect,
Golden yellow finger gushing water horsefly is can be utilized to process various corruption organic wastes, such as beer residue, bean dregs, chicken manure, pig manure and changing food waste etc..
Existing golden yellow finger gushing water horsefly kind all has preferable biological treatment capabilities to nontoxic organic waste, but to inapplicable fresh tobacco leaf
Treatment effect is not good.Main cause is because containing the toxicants such as a certain amount of nicotine in inapplicable fresh tobacco leaf, to golden yellow finger
Gushing water horsefly larva (golden yellow finger gushing water horsefly mature larva) has certain murder by poisoning and the effect of Developing restraint.In order to using golden yellow finger
The gushing water horsefly inapplicable fresh tobacco leaf of biological treatment, need to dash forward from the wild golden yellow golden yellow finger referring to filter out resistance to nicotine gushing water horsefly kind first
Stratiomyiid kind, improves the golden yellow finger tolerance to fresh tobacco leaf for the gushing water horsefly.
The present invention provides a kind of resistance to nicotine the golden yellow breeding method referring to gushing water horsefly strain, comprises the steps:
The golden yellow finger gushing water of the nicotine aqueous formulation material being s1. 3 ‰ by tolerance mass percent concentration under indoor conditionss
The ovum of horsefly kind is seeded in the first formulation material, by worm's ovum cultivate to larva aging, pupate；
S2. golden yellow finger gushing water horsefly pupa feeds its adult using the second formulation material after sprouting wings, after its spawning, under entrance all one's life
The acclimatization culture in generation；
S3. press above-mentioned steps repetitive operation, the continuous domestication through 20～30 generations is cultivated, obtain stable resistance to nicotine gold
Huang refers to gushing water horsefly strain；
Wherein, described first formulation material is grouped into by each group of following percetage by weight: wheat bran 70～90%, imported fish meal
10～30%, with the nicotine aqueous solution of mass percent concentration 3 ‰, above-mentioned formulation material being adjusted to humidity is 70%；
Second formulation material is the aqueous solution of nicotine mass percent concentration 2 ‰～3 ‰.
Preferably, described first formulation material is grouped into by each group of following percetage by weight: wheat bran 80%, imported fish meal
20%, with the nicotine aqueous solution of mass percent concentration 3 ‰, above-mentioned formulation material being adjusted to humidity is 70%.
Preferably, the second formulation material is the aqueous solution of nicotine mass percent concentration 2.5 ‰.
Described nicotine can adopt the commercial products of purity >=99.5%.
The compound method of described first formulation material is by after each component mixing of the first formulation material, adds extra fine quality percentage
The humidity of formulation material is adjusted to 70% by the nicotine aqueous solution of specific concentration.
Golden yellow finger gushing water horsefly ovum 0.5～1.0 g is accessed among described every 1 kg the first formulation material.Preferably described every 1 kg
Golden yellow finger gushing water horsefly ovum 0.8 g is accessed in one formulation material.
Described tolerance mass percent concentration is the resistance to nicotine golden yellow finger gushing water horsefly kind of 3 ‰ nicotine aqueous formulation material
Refer to filter out resistance to nicotine golden yellow finger gushing water horsefly kind from wild golden yellow finger gushing water horsefly kind first, then will screen again
Resistance to nicotine golden yellow refer to gushing water horsefly kind carry out hot housing so as to tolerance mass percent concentration be 3 ‰ nicotine aqueous solution
Formulation material.
Described golden yellow refer to filter out that resistance to nicotine is golden yellow to refer to gushing water horsefly kind gushing water horsefly kind from wild method particularly includes:
S01. configuration traps compost, will trap compost and load in open containers；
Described trap each component that compost contains following percetage by weight: wheat bran 70～90%, imported fish meal 10～30%,
With the nicotine aqueous solution of mass percent concentration 1 ‰～2 ‰, above-mentioned formulation material being adjusted to humidity is 70%；
S02., when weather is fine, the container that morning 8:00～9:00 will be equipped with trapping compost is put in house refuse respectively
Trapping wild worm's ovum, afternoon, 17:00～18:00 withdrew the container being put in everywhere for heap, chicken house and pig farm；
S03. by reclaim each container be placed on indoor cultivation, wait the wild worm's ovum trapping cultivate to larva aging when, profit
Refer to golden yellow finger gushing water horsefly larva being separated with formulation material from food phase behavior of gushing water horsefly mature larva with golden yellow；
S04. pass through the golden yellow Morphological Identification referring to gushing water horsefly larva, choose golden yellow finger gushing water horsefly mature larva；
S05. filter out 20～25 days aging, every golden yellow refer to gushing water horsefly larva and weigh that 0.10～0.16 g, figure be plump, face
The pitch-black golden yellow finger gushing water horsefly mature larva of color, at ambient temperature, allows golden yellow finger gushing water horsefly mature larva naturally to pupate, to sprout wings
The golden yellow finger gushing water horsefly kind of resistance to nicotine can be obtained afterwards.
Described by golden yellow for the resistance to nicotine screening finger gushing water horsefly kind carry out hot housing so as to tolerance mass percent
Concentration is 3 ‰ nicotine aqueous formulation material method particularly includes:
S11. the starting stage: golden yellow for the resistance to nicotine ovum referring to gushing water horsefly kind is seeded in initial incubation material, described initial
Compost is grouped into by each group of following percetage by weight: wheat bran 70～90%, imported fish meal 10～30%, dense with mass percent
Above-mentioned formulation material is adjusted to humidity for 70% by the nicotine aqueous solution spending 1 ‰；In room conditions, above-mentioned worm's ovum is cultivated to larva
Aging, pupate, sprout wings after, feed its adult；
S12. the 1st strain: after the Adult worms producting eggs of s11, the ovum being produced is entered using the 1st hot housing material
Row cultivates culture；Described 1st hot housing material contains each component of following percetage by weight: wheat bran 70～90%, imported fish meal
10～30%, with the nicotine aqueous solution of mass percent concentration (1+s) ‰, above-mentioned formulation material is adjusted to the value that humidity is 70%, s
Selected from 0.1～0.5；In room conditions, by above-mentioned worm's ovum cultivate to larva aging, pupate, sprout wings after, feed its adult；
S13. (n+1)th strain: again hot housing is carried out to the ovum of the adult product of n-th strain gained,
And the component contained by compost used by (n+1)th strain meets following ratio: the mass percent of nicotine aqueous solution is dense
Degree=(n-th cultivates the content+s of nicotine in compost) ‰, remaining constituent content is constant, and n is the natural number more than or equal to 1, weight
Multiple strengthen, till the mass percent concentration of nicotine aqueous solution reaches 3 ‰ in the compost described in last breeding phase.
Described its adult of nursing is all to be fed using clear water.Preferably, described clear water may be selected pure water, tap water, well
Water or nontoxic river etc.；The mode that water is fed is to be saturated with water using sponge, sucks for adult.
Preferably, the described indoor conditionss cultivating golden yellow finger gushing water horsefly are 25～30 DEG C of temperature, humidity 30～70%.
The invention has the beneficial effects as follows:
The present invention creatively directly adopts nicotine aqueous solution and wheat bran and the imported fish meal scientific compatibility of suitable concentration, obtains
The first formulation material obtaining not only successfully covers the unpleasant of nicotine and bitterness, and nutritional labeling is suitably it is ensured that golden yellow refer to gushing water horsefly larva
Growth is smooth, and because the appropriateness of the first formulation material and the second formulation material nicotine content and definitiveness, and the first formula
The reasonability that arranges before and after material and the second formulation material nutrient substance and nicotine content is it is ensured that in same nurturing an environment, different
The golden yellow finger gushing water horsefly mature larva rate that the formulation material of the same weight composition of batch obtains is essentially identical, obtains stable cultivation
Effect.
Cultivate the golden yellow gushing water horsefly strain that refers to of the resistance to nicotine obtaining through the present invention due to have passed through taming and dociling for a long time of 20～30 generations
Change cultivate, not only adapted to culture conditions, and defined more stable hereditary character, its offspring resistance to nicotine ability steady
Qualitative fine, and cultivate the golden yellow gushing water horsefly strain that refers to of the resistance to nicotine obtaining without the present invention and (only pass through screening and hot housing
Golden yellow finger gushing water horsefly) offspring easily degenerates to the toleration of nicotine, stability is bad.
Specific embodiment
Further describe the present invention with reference to specific embodiment.Unless stated otherwise, the present invention adopt reagent,
Equipment is the art conventional reagent and equipment.
Nicotine is purchased from conventional chemical reagent shop, and purity is >=99.5%.
Embodiment 1 filters out resistance to nicotine golden yellow finger gushing water horsefly kind from wild golden yellow finger gushing water horsefly kind
S01. when weather is fine, prepares and trap compost, described trap each component by following percetage by weight for the compost
Composition: wheat bran 90%, imported fish meal 10%, with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material is adjusted to humidity
For 70%.
S02. by the above-mentioned compost modulating, every 1 kg loads (long * width * height=45cm*30cm* in a square plastic feed basin
12cm).It is put in by a life rubbish heap in the plastic tub that the morning on same day 8:00 will be equipped with compost, afternoon, 17:00 withdrew everywhere
Plastic tub, takes back indoor cultivation.
S03. in room conditions (25 DEG C of temperature, humidity 30%), the wild worm's ovum trapping is cultivated aging to larva,
Using golden yellow refer to golden yellow finger gushing water horsefly larva being separated with formulation material from food phase behavior of gushing water horsefly mature larva (i.e. golden yellow refer to prominent
Can voluntarily climb when stratiomyiid mature larva is aging from compost, find dry place and pupate).
S04. by the Morphological Identification of larva under anatomical lens, individually choose the golden yellow finger aging children of gushing water horsefly with tweezers
Worm, excludes other fly kinds.
S05. filter out 20～25 days aging, every golden yellow refer to gushing water horsefly larva and weigh that 0.10～0.16 g, figure be plump, face
The pitch-black golden yellow finger gushing water horsefly mature larva of color, at ambient temperature, allow these golden yellow finger gushing water horsefly mature larvas naturally to pupate,
The golden yellow finger gushing water horsefly kind of resistance to nicotine can be obtained after emergence.
Embodiment 2 filters out resistance to nicotine golden yellow finger gushing water horsefly kind from wild golden yellow finger gushing water horsefly kind
S01. when weather is fine, prepares and trap compost, described trap each component by following percetage by weight for the compost
Composition: wheat bran 80%, imported fish meal 20%, with the nicotine aqueous solution of mass percent concentration 1.5 ‰, above-mentioned formulation material is adjusted to wet
Spend for 70%.
S02. by the above-mentioned compost modulating, every 1kg loads (long * width * height=45cm*30cm* in a square plastic feed basin
12cm).It is put in by a life rubbish heap in the plastic tub that the morning on same day 8:00 will be equipped with compost, afternoon, 17:00 withdrew everywhere
Plastic tub, takes back indoor cultivation.
S03. in room conditions (25 DEG C of temperature, humidity 30%), the wild worm's ovum trapping is cultivated aging to larva,
Using golden yellow refer to golden yellow finger gushing water horsefly larva being separated with formulation material from food phase behavior of gushing water horsefly mature larva (i.e. golden yellow refer to prominent
Can voluntarily climb when stratiomyiid mature larva is aging from compost, find dry place and pupate).
S04. by the Morphological Identification of larva under anatomical lens, individually choose the golden yellow finger aging children of gushing water horsefly with tweezers
Worm, excludes other fly kinds.
S05. filter out 20～25 days aging, every golden yellow refer to gushing water horsefly larva and weigh that 0.10～0.16 g, figure be plump, face
The pitch-black golden yellow finger gushing water horsefly mature larva of color, at ambient temperature, allow these golden yellow finger gushing water horsefly mature larvas naturally to pupate,
The golden yellow finger gushing water horsefly kind of resistance to nicotine can be obtained after emergence.
Embodiment 3 filters out resistance to nicotine golden yellow finger gushing water horsefly kind from wild golden yellow finger gushing water horsefly kind
S01. when weather is fine, prepares and trap compost, described trap each component by following percetage by weight for the compost
Composition: wheat bran 70%, imported fish meal 30%, with the nicotine aqueous solution of mass percent concentration 2 ‰, above-mentioned formulation material is adjusted to humidity
For 70%.
S02. by the above-mentioned compost modulating, every 1 kg loads (long * width * height=45cm*30cm* in a square plastic feed basin
12cm).It is put in by a life rubbish heap in the plastic tub that the morning on same day 8:00 will be equipped with compost, afternoon, 17:00 withdrew everywhere
Plastic tub, takes back indoor cultivation.
S03. in room conditions (25 DEG C of temperature, humidity 30%), the wild worm's ovum trapping is cultivated aging to larva,
Using golden yellow refer to golden yellow finger gushing water horsefly larva being separated with formulation material from food phase behavior of gushing water horsefly mature larva (i.e. golden yellow refer to prominent
Can voluntarily climb when stratiomyiid mature larva is aging from compost, find dry place and pupate).
S04. by the Morphological Identification of larva under anatomical lens, individually choose the golden yellow finger aging children of gushing water horsefly with tweezers
Worm, excludes other fly kinds.
S5. filter out 20～25 days aging, every golden yellow refer to gushing water horsefly larva and weigh that 0.10～0.16 g, figure be plump, face
The pitch-black golden yellow finger gushing water horsefly mature larva of color, at ambient temperature, allow these golden yellow finger gushing water horsefly mature larvas naturally to pupate,
The golden yellow finger gushing water horsefly kind of resistance to nicotine can be obtained after emergence.
Embodiment 4 finger golden yellow to the resistance to nicotine screening gushing water horsefly kind carries out hot housing makes it tolerate quality hundred
Divide the formulation material of the nicotine aqueous solution that specific concentration is 3 ‰
Resistance to nicotine that embodiment 1～3 any embodiment is screened is golden yellow to be referred to gushing water horsefly kind and is cultivated so as to resistance to
The formulation material of the nicotine aqueous solution being 3 ‰ by mass percent concentration, concrete grammar is as follows:
S11. the starting stage:
Prepare initial incubation material, described initial incubation material is grouped into by each group of following percetage by weight: wheat bran 90%, enter
Mouth fish flour 10%, with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 70%.By above-mentioned modulation
Good compost, every 1 kg loads in a square plastic feed basin (long * width * height=45cm*30cm*12cm), and it is prominent that every basin accesses golden yellow finger
Stratiomyiid ovum 0.8 g；In room conditions (25～30 DEG C of temperature, humidity 30～70%), by above-mentioned worm's ovum cultivate to larva aging,
After pupating, sprouting wings, feed its adult.
Strain:
S12. the 1st strain: after the Adult worms producting eggs of starting stage, the ovum being produced adopts the 1st strengthening training
Nutriment carries out hot housing；Described 1st hot housing material contains each component of following percetage by weight: wheat bran 80%, import fish
Powder 20%, with the nicotine aqueous solution of mass percent concentration (1+s) ‰, above-mentioned formulation material being adjusted to humidity is that 70%, s is selected from 0.1
～0.5；In room conditions (25～30 DEG C of temperature, humidity 30～70%), by above-mentioned worm's ovum cultivate to larva aging, pupate, plumage
After change, feed its adult.
S13. (n+1)th strain: again hot housing is carried out to the ovum of the adult product of n-th strain gained,
And the component contained by compost used by (n+1)th strain meets following ratio: the mass percent of nicotine aqueous solution is dense
Degree=(n-th cultivates the content+s of nicotine in compost) ‰, remaining constituent content is constant, and n is the natural number more than or equal to 1, weight
Multiple strengthen, till the mass percent concentration of nicotine aqueous solution reaches 3 ‰ in the compost described in last strain.
Preferably, in this enforcement, in whole breeding phase, the mass percent concentration of nicotine aqueous solution is followed successively by
1.1‰、1.2‰、1.3‰、1.4‰、1.5‰、1.6‰、1.7‰、1.8‰、1.9‰、2.0‰、2.1‰、2.2‰、
2.3‰、2.4‰、2.5‰、2.6‰、2.7‰、2.8‰、2.9‰、3.0‰；Namely golden yellow finger gushing water horsefly ovum experience 20 times altogether
Hot housing.The golden yellow finger gushing water horsefly kind that this stage turns out has higher tolerance to nicotine.
Embodiment 5 finger golden yellow to the resistance to nicotine screening gushing water horsefly kind carries out hot housing makes it tolerate quality hundred
Divide the formulation material of the nicotine aqueous solution that specific concentration is 3 ‰
Resistance to nicotine that embodiment 1～3 any embodiment is screened is golden yellow to be referred to gushing water horsefly kind and is cultivated so as to resistance to
The formulation material of the nicotine aqueous solution being 3 ‰ by mass percent concentration, concrete grammar is as follows:
S11. the starting stage:
Prepare initial incubation material, described initial incubation material is grouped into by each group of following percetage by weight: wheat bran 80%, enter
Mouth fish flour 20%, with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 70%.By above-mentioned modulation
Good compost, every 1 kg loads in a square plastic feed basin (long * width * height=45cm*30cm*12cm), and it is prominent that every basin accesses golden yellow finger
Stratiomyiid ovum 0.8 g；In room conditions (25～30 DEG C of temperature, humidity 30～70%), by above-mentioned worm's ovum cultivate to larva aging,
After pupating, sprouting wings, feed its adult.
Strain:
S12. the 1st strain: after the Adult worms producting eggs of starting stage, the ovum being produced adopts the 1st strengthening training
Nutriment carries out hot housing；Described 1st hot housing material contains each component of following percetage by weight: wheat bran 80%, import fish
Powder 20%, with the nicotine aqueous solution of mass percent concentration (1+s) ‰ by above-mentioned formulation material be adjusted to humidity be 70%, s be selected from 0.1～
0.5；In room conditions (25～30 DEG C of temperature, humidity 30～70%), by above-mentioned worm's ovum cultivate to larva aging, pupate, sprout wings
Afterwards, feed its adult；
S13. (n+1)th strain: again hot housing is carried out to the ovum of the adult product of n-th strain gained,
And the component contained by compost used by (n+1)th strain meets following ratio: the mass percent of nicotine aqueous solution is dense
Degree=(n-th cultivates the content+s of nicotine in compost) ‰, remaining constituent content is constant, and n is the natural number more than or equal to 1, weight
Multiple strengthen, till the mass percent concentration of nicotine aqueous solution reaches 3 ‰ in the compost described in last strain.
Preferably, in this enforcement, in whole breeding phase, the mass percent concentration of nicotine aqueous solution is followed successively by
1.2‰、1.4‰、1.6‰、1.8‰、2.0‰、2.2‰、2.4‰、2.6‰、2.8‰、3.0‰；Namely golden yellow finger gushing water horsefly
Ovum experiences 10 hot housing altogether.The golden yellow finger gushing water horsefly kind that this stage turns out has higher tolerance to nicotine.
Embodiment 6 finger golden yellow to the resistance to nicotine screening gushing water horsefly kind carries out hot housing makes it tolerate quality hundred
Divide the formulation material of the nicotine aqueous solution that specific concentration is 3 ‰
Resistance to nicotine that embodiment 1～3 any embodiment is screened is golden yellow to be referred to gushing water horsefly kind and is cultivated so as to resistance to
The formulation material of the nicotine aqueous solution being 3 ‰ by mass percent concentration, concrete grammar is as follows:
S11. the starting stage:
Prepare initial incubation material, described initial incubation material is grouped into by each group of following percetage by weight: wheat bran 70%, enter
Mouth fish flour 30%, with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 70%.By above-mentioned modulation
Good compost, every 1 kg loads in a square plastic feed basin (long * width * height=45cm*30cm*12cm), and it is prominent that every basin accesses golden yellow finger
Stratiomyiid ovum 0.8 g；In room conditions (25～30 DEG C of temperature, humidity 30～70%), by above-mentioned worm's ovum cultivate to larva aging,
After pupating, sprouting wings, feed its adult.Described its adult of nursing is all to be fed using clear water.Described clear water may be selected pure water, from
Water, well water or nontoxic river etc.；The mode that water is fed is to be saturated with water using sponge, sucks for adult.Similarly hereinafter.
Strain:
S12. the 1st strain: after the Adult worms producting eggs of starting stage, the ovum being produced adopts the 1st strengthening training
Nutriment carries out hot housing；Described 1st hot housing material contains each component of following percetage by weight: wheat bran 80%, import fish
Powder 20%, with the nicotine aqueous solution of mass percent concentration (1+s) ‰, above-mentioned formulation material being adjusted to humidity is that 70%, s is selected from 0.1
～0.5；In room conditions (25～30 DEG C of temperature, humidity 30～70%), by above-mentioned worm's ovum cultivate to larva aging, pupate, plumage
After change, feed its adult；
S13. (n+1)th strain: again hot housing is carried out to the ovum of the adult product of n-th strain gained,
And the component contained by compost used by (n+1)th strain meets following ratio: the mass percent of nicotine aqueous solution is dense
Degree=(n-th cultivates the content+s of nicotine in compost) ‰, remaining constituent content is constant, and n is the natural number more than or equal to 1, weight
Multiple strengthen, till the mass percent concentration of nicotine aqueous solution reaches 3 ‰ in the compost described in last strain.
Preferably, in this enforcement, in whole breeding phase, the mass percent concentration of nicotine aqueous solution is followed successively by
1.5‰、2.0‰、2.5‰、3.0‰；Namely golden yellow finger gushing water horsefly ovum experience 4 hot housing altogether.The gold that this stage turns out
Huang refers to gushing water horsefly kind and has higher tolerance to nicotine.
The continuous domestication of more than 7 generation of embodiment is cultivated and is obtained stable resistance to nicotine golden yellow finger gushing water horsefly strain
The nicotine aqueous solution that mass percent concentration is 3 ‰ that tolerates that embodiment 4～6 any embodiment is cultivated is joined
Cultivate again by the continuous domestication through multiple generations for the golden yellow finger gushing water horsefly kind of square stock, forms stable resistance to nicotine golden yellow finger gushing water
Horsefly strain.Method particularly includes:
S1. under indoor conditionss, the golden yellow finger gushing water of the nicotine aqueous formulation material that tolerance mass percent concentration is 3 ‰
The ovum of horsefly kind is seeded in the first formulation material, by worm's ovum cultivate to larva aging, pupate；
S2. golden yellow finger gushing water horsefly pupa feeds its adult using the second formulation material after sprouting wings, after its spawning, under entrance all one's life
The acclimatization culture in generation；
S3. press above-mentioned steps repetitive operation, the continuous domestication through 30 generations is cultivated, and obtains the stable golden yellow finger of resistance to nicotine
Gushing water horsefly strain；
The preparation of the first formulation material: described first formulation material is grouped into by each group of following percetage by weight: wheat bran 90%,
Imported fish meal 10%, with the nicotine aqueous solution of mass percent concentration 3 ‰, above-mentioned formulation material being adjusted to humidity is 70%.
By the above-mentioned formulation material modulating, every 1 kg loads (long * width * height=45cm*30cm* in a square plastic feed basin
12cm), every basin accesses golden yellow finger gushing water horsefly ovum 0.8 g；
In room conditions (25～30 DEG C of temperature, humidity 30～70%), by above-mentioned worm's ovum cultivate to larva aging, pupate；
Golden yellow finger gushing water horsefly pupa feeds its adult using the second formulation material after sprouting wings, and the second formulation material is nicotine percent mass
The aqueous solution of specific concentration 2 ‰, after its spawning, enters the acclimatization culture of next generation；
By above-mentioned steps repetitive operation, the continuous domestication through 30 generations is cultivated, and obtains the golden yellow finger of stable resistance to nicotine prominent
Stratiomyiid strain.
Embodiment 8
Other operations are grouped into by each group of following percetage by weight with embodiment 7, except for the difference that described first formulation material:
Wheat bran 80%, imported fish meal 20%, with the nicotine aqueous solution of mass percent concentration 3 ‰, above-mentioned formulation material being adjusted to humidity is 70%.
Described second formulation material is the aqueous solution of nicotine mass percent concentration 2.5 ‰.Continuous domestication through 25 generations is cultivated, and obtains
Stable resistance to nicotine is golden yellow to refer to gushing water horsefly strain.
Embodiment 9
Other operations are grouped into by each group of following percetage by weight with embodiment 7, except for the difference that described first formulation material:
Wheat bran 70%, imported fish meal 30%, with the nicotine aqueous solution of mass percent concentration 3 ‰, above-mentioned formulation material being adjusted to humidity is 70%.
Described second formulation material is the aqueous solution of nicotine mass percent concentration 3 ‰.Continuous domestication through 20 generations is cultivated, and obtains steady
Fixed resistance to nicotine is golden yellow to refer to gushing water horsefly strain.
Comparative example 1
Operation is grouped into by each group of following percetage by weight with embodiment 1, the except for the difference that described compost that traps: wheat bran
80% and imported fish meal 20%.Experiment finds using as above trapping the kind that the ovum that compost traps is not resistance to nicotine.
Embodiment 10
The golden yellow finger gushing water horsefly kind that the embodiment of the present invention 1 to 3 is screened, embodiment 4 to 6 tolerate through culture
The golden yellow finger gushing water horsefly kind of 3 ‰ nicotine formulation material, embodiment 7 to 9 are through the excessively golden yellow finger gushing water for continuous domestication culture
Golden yellow finger gushing water horsefly kind after horsefly kind, each comparative example are processed carries out resistance to nicotine aptitude tests, and test process is:
The ovum 0.5g that golden yellow finger gushing water horsefly adult after taking described embodiment culture to process respectively is produced, is respectively connected to 1kg
Weight the nicotine aqueous solution of percent concentration containing better quality formulation material (wheat bran 80%, imported fish meal 20%, dense with mass percent
Above-mentioned formulation material is adjusted to humidity for 70% by the nicotine aqueous solution spending 3.0 ‰) in, test result is shown in Table 1:
Table 1
Test result illustrates, comparative example 1 is not resistant to the nicotine aqueous solution of mass percent concentration 3.0 ‰.
1 to 3 golden yellow finger gushing water horsefly kind through screening of embodiment is in the nicotine aqueous solution of mass percent concentration 3.0 ‰
Diapause phenomenon is there occurs, larval weight is lighter, and has relatively large death in the formulation material of modulation.At embodiment 4 to 9 hot housing
The resistance to nicotine ability of the golden yellow finger gushing water horsefly kind after reason greatly improves, and can tolerate the nicotine of mass percent concentration 3.0 ‰
The formulation material of aqueous solution modulation.
Embodiment 11 stability experiment
Golden yellow for the resistance to nicotine obtaining through embodiment 4 to 6 methods described finger gushing water horsefly kind is carried out conventional cultivation (wherein gold
It is wheat bran 80%+ fish flour 20% that Huang refers to gushing water horsefly larva culture material formula, and with clear water, above-mentioned formulation material being adjusted to humidity is 70%, becomes
Worm feedstuff is clear water) standby after 25 generations.Meanwhile, by golden yellow for the resistance to nicotine obtaining through embodiment 7 to 9 methods described finger gushing water horsefly product
Using cultivating material, (wherein golden yellow finger gushing water horsefly larva culture material formula is wheat bran 80%, imported fish meal 20%, uses mass percent for system
It is 70% that above-mentioned formulation material is adjusted to humidity by the nicotine aqueous solution of concentration 3.0 ‰, and adult forage is mass percent concentration 2.5 ‰
Nicotine aqueous solution) persistently cultivate again standby after 25 generations.The golden yellow finger gushing water horsefly kind of above-mentioned two strain is carried out the energy of resistance to nicotine
Power is tested, and detection culture material formula is wheat bran 80%, imported fish meal 20%, with the nicotine aqueous solution of mass percent concentration 3 ‰
The humidity that above-mentioned formulation material is adjusted to formulation material is 70%, detects whether the resistance to nicotine ability of its offspring declines.Test result is shown in Table
Shown in 2:
The stability experiment result of golden yellow finger gushing water horsefly strain after 25 generations for the table 2
| Process | Larvae development go through the phase (my god) | Larva bar number/g(bar) | Survival rate of larvae (%) |
| The conventional strain of embodiment 4 | 7 | 48 | 63 |
| The conventional strain of embodiment 5 | 7 | 47 | 66 |
| The conventional strain of embodiment 6 | 7 | 48 | 64 |
| Embodiment 7 cultivates strain | 5 | 28 | 96 |
| Embodiment 8 cultivates strain | 5 | 28 | 96 |
| Embodiment 9 cultivates strain | 5 | 29 | 95 |
Result shows the golden yellow gushing water horsefly kind that refers to of the resistance to nicotine after strengthening after cultivating for 25 generations with cellar culture material, resistance to
Nicotine ability there occurs serious degradation phenomenon, and cultivates the resistance to nicotine obtaining golden yellow finger gushing water horsefly lines progenies pair through the present invention
The stability of nicotine tolerance is fine, and in inventive formulation material compositing range, the formula of the same weight composition of different batches
The golden yellow finger gushing water horsefly that material obtains has stable cultivate effect.
Claims (8)
1. a kind of golden yellow breeding method referring to gushing water horsefly strain of resistance to nicotine is it is characterised in that comprise the steps:
S1. under indoor conditionss, the golden yellow finger gushing water horsefly product of the nicotine aqueous formulation material that tolerance mass percent concentration is 3 ‰
Kind ovum be seeded in the first formulation material, by worm's ovum cultivate to larva aging, pupate；
S2. fly pupa feeds its adult using the second formulation material after sprouting wings, and after its spawning, enters the acclimatization culture of next generation；
S3. press above-mentioned steps repetitive operation, the continuous domestication through 20～30 generations is cultivated, and obtains the stable golden yellow finger of resistance to nicotine
Gushing water horsefly strain；
Wherein, described first formulation material is grouped into by each group of following percetage by weight: wheat bran 80%, imported fish meal 20%, uses
It is 70% that above-mentioned first formulation material is adjusted to humidity by the nicotine aqueous solution of mass percent concentration 3 ‰；
Second formulation material is the nicotine aqueous solution of mass percent concentration 2 ‰～3 ‰.
2. breeding method is it is characterised in that indoor conditionss described in s1 are 25～30 DEG C of temperature, wet according to claim 1
Degree 30～70%.
3. according to claim 1 breeding method it is characterised in that the second formulation material is mass percent concentration 2.5 ‰
Nicotine aqueous solution.
4. according to claim 1 breeding method it is characterised in that the compound method of described first formulation material is to join first
After each component of square stock mixes, with the nicotine aqueous solution of mass percent concentration 3 ‰, above-mentioned first formulation material being adjusted to humidity is
70%.
5. according to claim 1 breeding method it is characterised in that accessing golden yellow finger among the first formulation material described in every 1kg
Gushing water horsefly ovum 0.5～1.0g.
6. according to claim 1 breeding method it is characterised in that described tolerance mass percent concentration is 3 ‰ nicotine
The preparation method of the golden yellow finger gushing water horsefly kind of aqueous formulation material is: filters out from wild golden yellow finger gushing water horsefly kind first
Resistance to nicotine is golden yellow to refer to gushing water horsefly kind, then more golden yellow for the resistance to nicotine screening finger gushing water horsefly kind is carried out hot housing,
Make it tolerate nicotine aqueous formulation material that mass percent concentration is 3 ‰.
7. according to claim 6 breeding method it is characterised in that the golden yellow screening referring to gushing water horsefly kind of described resistance to nicotine
Method is:
S1. configuration traps compost, will trap compost and load in open containers；
Described trap each component that compost contains following percetage by weight: wheat bran 80%, imported fish meal 20%, use percent mass
The nicotine aqueous solution of specific concentration 1 ‰～2 ‰ trap compost to be adjusted to humidity be 70% by above-mentioned；
S2., when weather is fine, the container that will be equipped with trapping compost in the morning is put in house refuse heap, chicken house and pig farm to lure respectively
Collect wild golden yellow finger gushing water Tabanus ovum, withdraw the container being put in everywhere afternoon；
S3. by reclaim each container be placed on indoor cultivation, wait the wild worm's ovum trapping cultivate to larva aging when, using golden yellow
Refer to golden yellow finger gushing water horsefly larva being separated from food phase behavior of gushing water horsefly maggot with trapping compost；
S4. pass through the golden yellow Morphological Identification referring to gushing water horsefly larva, choose golden yellow finger gushing water horsefly maggot；
S5. filter out 20～25 days aging, every golden yellow refer to gushing water horsefly larva and weigh that 0.10～0.16g, figure be plump, color is pitch-black
Golden yellow finger gushing water horsefly maggot, at ambient temperature, allow golden yellow finger gushing water horsefly maggot naturally pupate, sprout wings after can obtain resistance to nicotine
Golden yellow finger gushing water horsefly kind.
8. according to claim 6 breeding method it is characterised in that described resistance to nicotine golden yellow refer to gushing water horsefly kind strengthening training
Foster method is:
Starting stage: by golden yellow for resistance to nicotine refer to gushing water horsefly kind ovum be seeded in initial incubation material, described initial incubation material by
The each group of following percetage by weight is grouped into: wheat bran 80%, imported fish meal 20%, with the nicotine water of mass percent concentration 1 ‰
It is 70% that above-mentioned initial incubation material is adjusted to humidity by solution；In room conditions, by above-mentioned ovum cultivate to larva aging, pupate,
After emergence, feed adult；
1st strain: after the Adult worms producting eggs of starting stage, the ovum being produced is carried out using the 1st hot housing material
Hot housing；Described 1st hot housing material is grouped into by each group of following percetage by weight: wheat bran 80%, imported fish meal
20%, with the nicotine aqueous solution of mass percent concentration (1+s) ‰, above-mentioned 1st hot housing material being adjusted to humidity is 70%, s
Selected from 0.1～0.5；In room conditions, by above-mentioned ovum cultivate to larva aging, pupate, sprout wings after, feed adult；
(n+1)th strain: again hot housing is carried out to the ovum of the adult product of n-th strain gained, and (n+1)th
Component contained by compost used by secondary strain meets following ratio: the mass percent concentration of nicotine aqueous solution=(n-th
Content+the s of nicotine in hot housing material) ‰, remaining constituent content is constant, and n is the natural number more than or equal to 1, repeats to strengthen, directly
Reaching 3 ‰ to the mass percent concentration of nicotine aqueous solution in compost described in last strain.
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| CN103651263B (en) * | 2013-11-07 | 2015-12-09 | 中山大学 | A kind of breeding method of resistance to nicotine housefly strain |
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