CN104285898B - A kind of black of resistance to nicotine refers to the intensifying method of gushing water horsefly kind - Google Patents
A kind of black of resistance to nicotine refers to the intensifying method of gushing water horsefly kind Download PDFInfo
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- CN104285898B CN104285898B CN201410421796.8A CN201410421796A CN104285898B CN 104285898 B CN104285898 B CN 104285898B CN 201410421796 A CN201410421796 A CN 201410421796A CN 104285898 B CN104285898 B CN 104285898B
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- nicotine
- black
- gushing water
- ovum
- aqueous solution
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- SNICXCGAKADSCV-JTQLQIEISA-N (-)-Nicotine Chemical compound CN1CCC[C@H]1C1=CC=CN=C1 SNICXCGAKADSCV-JTQLQIEISA-N 0.000 title claims abstract description 128
- 229960002715 nicotine Drugs 0.000 title claims abstract description 128
- SNICXCGAKADSCV-UHFFFAOYSA-N nicotine Natural products CN1CCCC1C1=CC=CN=C1 SNICXCGAKADSCV-UHFFFAOYSA-N 0.000 title claims abstract description 128
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 111
- 241000255632 Tabanus atratus Species 0.000 title claims abstract description 94
- 238000000034 method Methods 0.000 title claims abstract description 28
- 239000000463 material Substances 0.000 claims abstract description 69
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 48
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 48
- 239000007864 aqueous solution Substances 0.000 claims abstract description 48
- 210000004681 ovum Anatomy 0.000 claims abstract description 48
- 238000011534 incubation Methods 0.000 claims abstract description 21
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 20
- 235000019733 Fish meal Nutrition 0.000 claims abstract description 17
- 239000004467 fishmeal Substances 0.000 claims abstract description 17
- 238000012216 screening Methods 0.000 claims abstract description 7
- 239000002361 compost Substances 0.000 claims description 50
- 239000000203 mixture Substances 0.000 claims description 36
- 230000032683 aging Effects 0.000 claims description 26
- 230000008859 change Effects 0.000 claims description 5
- 239000000470 constituent Substances 0.000 claims description 5
- 235000013601 eggs Nutrition 0.000 claims description 5
- 235000013305 food Nutrition 0.000 claims description 5
- 230000000877 morphologic effect Effects 0.000 claims description 5
- 238000012549 training Methods 0.000 claims description 4
- 241000287828 Gallus gallus Species 0.000 claims description 3
- 231100000252 nontoxic Toxicity 0.000 claims description 3
- 230000003000 nontoxic effect Effects 0.000 claims description 3
- 229920006395 saturated elastomer Polymers 0.000 claims description 3
- 230000000474 nursing effect Effects 0.000 claims description 2
- 235000020681 well water Nutrition 0.000 claims description 2
- 239000002349 well water Substances 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 8
- 241000208125 Nicotiana Species 0.000 description 35
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 35
- 238000009472 formulation Methods 0.000 description 31
- 230000003014 reinforcing effect Effects 0.000 description 12
- 241000238631 Hexapoda Species 0.000 description 6
- 241000257159 Musca domestica Species 0.000 description 6
- 235000019504 cigarettes Nutrition 0.000 description 6
- 239000000843 powder Substances 0.000 description 5
- 238000009825 accumulation Methods 0.000 description 4
- 230000006399 behavior Effects 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 241000251468 Actinopterygii Species 0.000 description 3
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 230000008676 import Effects 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 244000046052 Phaseolus vulgaris Species 0.000 description 2
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 239000004202 carbamide Substances 0.000 description 2
- 230000005058 diapause Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 210000003608 fece Anatomy 0.000 description 2
- 230000004720 fertilization Effects 0.000 description 2
- 239000003337 fertilizer Substances 0.000 description 2
- 239000010871 livestock manure Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000010815 organic waste Substances 0.000 description 2
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 235000008939 whole milk Nutrition 0.000 description 2
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 description 1
- FFYVQVZXWDGRAY-UHFFFAOYSA-N 3-(1-methylpyrrolidin-2-yl)pyridine;hydrate Chemical compound O.CN1CCCC1C1=CC=CN=C1 FFYVQVZXWDGRAY-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000382353 Pupa Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000009418 agronomic effect Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 235000013405 beer Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 235000019658 bitter taste Nutrition 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000009571 larval growth Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 239000000618 nitrogen fertilizer Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 235000010333 potassium nitrate Nutrition 0.000 description 1
- 239000004323 potassium nitrate Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses the intensifying method that a kind of black of resistance to nicotine refers to gushing water horsefly kind.The wild black of resistance to nicotine for screening is referred to gushing water horsefly ovum culture to adult by the present invention using initial incubation material, the ovum of adult output is carried out into n times hot housing to adult using hot housing material, the mass percent concentration of the nicotine aqueous solution improves 0.1~0.5 point on the basis of upper once hot housing material in each hot housing circulation, until the mass percent concentration of the nicotine aqueous solution reaches 3 ‰.Directly using the nicotine aqueous solution and wheat bran and imported fish meal scientific compatibility of suitable concentration, success hot housing black refers to gushing water horsefly to the present invention, can by easy stages strengthen wild black and refer to tolerance of the gushing water horsefly kind to nicotine, obtains more stable cultivate effect.
Description
Technical field
The present invention relates to animal varieties triage techniques field, and in particular to a kind of black of resistance to nicotine refers to the strong of gushing water horsefly kind
Change method.
Background technology
Inapplicable fresh tobacco leaf refers to the fresh tobacco leaves for not being suitable for cured tobacco production（Water content 80~90%）, it is big with document
The waste/hypo-tobacco leaf or discarded tobacco leaf produced in the production process of flue-cured tobacco that amount is referred to（Water content about 15%）It is different.Inapplicable tobacco leaf leads to
After referring to that cigarette strain is normal and binding and remove 2~3 milk pin leaves, cigarette industry it is inapplicable, need to be removed under treatment in field
Portion's leaf and upper leaf, mainly include cigarette strain bottom because illumination deficiency, depauperation, inclusion is not enough, estimated roasting rear inferior quality,
Do not possess bad hacking at the top of 2 inferior leads of baking value, and cigarette strain, curtailment, structure is stiff, bake rear inferior quality
2 tops.
If these inapplicable tobacco leaves arbitrarily abandon field, environmental pollution and the repetition sense of tobacco virus are easily caused
Dye, destroys according to the concentration of prior art or the processing mode of landfill then needs to spend substantial amounts of expense, and current industry is per ton not
The processing cost mean height of tobacco leaf is applicable up to 200 yuan, each tobacco leaf producing region is generally required under the organization leadership of local government of party committee,
Policy propaganda, tabulation registration are carried out, treatment facility is built, unified removed, record archives, publicity, cashes and the measure such as subsidize and supervise
The treatment of inapplicable tobacco leaf is carried out, is wasted time and energy costly.
It is a kind of preferable alternate process method, ring to carry out biological treatment to inapplicable tobacco leaf using the insect of resistance to nicotine
Protect, high financial profit.In order to realize that biological treatment does not apply to fresh tobacco leaf using insect, need to filter out the insect product of resistance to nicotine first
Kind.
The applicant is based on studying housefly and inapplicable fresh tobacco leaf treatment technology for a long period of time, sums up using inapplicable fresh
The method that tobacco leaf carries out the screening, reinforcing and cultivation of resistance to nicotine strain to housefly.The reinforcing is the resistance to nicotine man that will be filtered out
The ovum of fly is inoculated in inapplicable fresh tobacco leaf compost culture to adult, then by the ovum of adult output using inapplicable fresh tobacco leaf reinforcing
Compost carries out n times hot housing to adult, and the ratio that inapplicable fresh tobacco leaf accounts for compost in each hot housing circulation all can
5~15 points are improved on the basis of upper once hot housing material, until inapplicable ratio of the fresh tobacco leaf in hot housing material
85% is reached, the housefly kind that this stage turns out has tolerance higher to nicotine.The inapplicable fresh tobacco leaf compost contains
There is each component of following percetage by weight：Inapplicable fresh tobacco leaf 30%, wheat bran 60%, imported fish meal 5%, dregs of beans 5%.
The technical scheme can go out the housefly kind of resistance to nicotine with successful incubation, but different batches cultivate the aging pre- of acquisition
The resistance to nicotine ability of pupa is also otherwise varied, there is the uncertainty of effect.Main reason is that the application aspect of inapplicable tobacco leaf,
Although cigarette can be reduced by the inapplicable tobacco leaf that creative research and many experiments sum up addition zone of reasonableness in formulation material
Toxic action of the alkali to common house-fly grub, but the factor of the inapplicable fresh tobacco leaf nicotine content of influence is very various, complicated mechanism, it is more difficult to
Realize accurate control.Influenceing the factor of nicotine content in tobacco leaf mainly includes：（1）Different growing stages, contained nicotine contains in tobacco leaf
Amount is different：In tobacco seed nicotine content be 0, risen in seedling raising process beginning formed in root, after developing with root system,
The synthesis of nicotine and accumulation ability gradually strengthen, so different growing stages, contained nicotine content is different in tobacco leaf；（2）Cigarette strain is not
Same position, nicotine content is dramatically different, and nicotine content is also by leaf position and number of sheets large effect；（3）Contained by different cultivars tobacco leaf
Nicotine amount is different；（4）Weather conditions of soil dry-wet etc. have the influence of different savings to nicotine content；（5）Agronomic measures
Difference significantly affects nicotine content of tobacco leaves, including fertilization type, planting density, pinches and smear wooden fork fertilising, girdling and cut root fertilizer, adopt
Debit's formula etc. factor all significantly affects the nicotine content in tobacco leaf, by taking fertilization type as an example, in three kinds of nitrogenous fertilizer of form, applies
After urea, nicotine accumulation highest, ammonium nitrate takes second place, and the nicotine accumulation of potassium nitrate treatment is minimum, lower by 27% than applying urea；To pinch
As a example by period, the research such as pellet finds, postpone nicotine accumulation intensity peak with Physical property declines successively, and nicotine content is also successively
Lower, often postpone and pinching for one day, nicotine content just declines 0.0504%；Again as a example by cutting root fertilizer, cut root 1/8 and do not cut root phase
Than upper leaf nicotine declines 1.9 percentage points of ranges of decrease 31.33%, and middle leaf nicotine declines 2.13 percentage points, the range of decrease 51.4%.
How to avoid using different batches compost cultivate obtain aging prepupa effect uncertainty be this area urgently
Technical barrier to be solved.And cultivate the more insect strain of resistance to nicotine of multi items and carry out using feasible technical scheme is stablized
Effectively reinforced cultivating, is this area important technological problems that needs are solved simultaneously.
The content of the invention
The technical problem to be solved in the present invention is to lack strong to the tolerance of nicotine resistance in the prior art to overcome
Nicotine black refers to the defect of gushing water horsefly kind, there is provided a kind of black of resistance to nicotine refers to the intensifying method of gushing water horsefly kind.
The purpose of the present invention is achieved by the following technical programs：
Black refers to that gushing water horsefly is another insect for being different from housefly, and it is a kind of important economic insects and environmentally friendly elder brother
Worm, refers to that gushing water horsefly larva processes various corrupt organic wastes, such as beer residue, bean dregs, chicken manure, pig manure and kitchen using black
Rubbish etc..Existing black refers to that gushing water horsefly kind has preferable biological treatment capabilities to nontoxic organic waste, but to inapplicable
The treatment effect of fresh tobacco leaf is not good.Main cause because containing toxicants such as a certain amount of nicotine in inapplicable fresh tobacco leaf,
Refer to black gushing water horsefly larva（Black refers to gushing water horsefly prepupa）Effect with certain murder by poisoning and Developing restraint.In order to using black
Color refers to that the biological treatment of gushing water horsefly does not apply to fresh tobacco leaf, need to refer to the black that resistance to nicotine is filtered out in gushing water horsefly kind from wild black first
Refer to gushing water horsefly kind, improve black and refer to tolerance of the gushing water horsefly to fresh tobacco leaf.
The present invention provides the intensifying method that a kind of black of resistance to nicotine refers to gushing water horsefly kind, comprises the following steps：
Starting stage：The ovum that the black of resistance to nicotine refers to gushing water horsefly kind is seeded in initial incubation material, the initial incubation
Each component of the material containing following percetage by weight：Wheat bran 70~90%, imported fish meal 10~30%, with mass percent concentration 1 ‰
The nicotine aqueous solution above-mentioned formulation material is adjusted to humidity for 70%；In room conditions, by above-mentioned worm's ovum cultivate to larva it is aging,
After pupating, sprouting wings, its adult is fed using water；
1st strain：The 1st hot housing material is used after the ovum for after the Adult worms producting eggs of starting stage, being produced
Carry out hot housing；The 1st hot housing material contains each component of following percetage by weight：Wheat bran 80%, imported fish meal
20%, with the nicotine aqueous solution of mass percent concentration (1+S) ‰ by above-mentioned formulation material be adjusted to humidity be selected from 0.1 for 70%, S~
0.5；In room conditions, by above-mentioned worm's ovum cultivate to larva it is aging, pupate, sprout wings after, feed its adult；
The N+1 times strain：Hot housing is carried out again to the ovum that the adult obtained by n-th strain produces, and
Component contained by compost used by the N+1 times strain meets following ratio：The mass percent concentration of the nicotine aqueous solution=
（Content+the S of nicotine in n-th hot housing material）‰, remaining constituent content is constant, and N is the natural number more than or equal to 1, repeats
Reinforcing, until the nicotine aqueous solution in compost described in last time strain mass percent concentration up to untill 3 ‰.This rank
The black that section is turned out refers to that gushing water horsefly kind has tolerance higher to nicotine.
Preferably, in whole strain, the mass percent concentration of the nicotine aqueous solution is followed successively by 1.1 ‰, 1.2 ‰,
1.3‰、1.4‰、1.5‰、1.6‰、1.7‰、1.8‰、1.9‰、2.0‰、2.1‰、2.2‰、2.3‰、2.4‰、
2.5‰、2.6‰、2.7‰、2.8‰、2.9‰、3.0‰；Namely black refers to that gushing water horsefly ovum experiences 20 hot housing altogether.
Preferably, in whole strain, the mass percent concentration of the nicotine aqueous solution is followed successively by 1.2 ‰, 1.4 ‰,
1.6‰、1.8‰、2.0‰、2.2‰、2.4‰、2.6‰、2.8‰、3.0‰；Namely black refers to that gushing water horsefly ovum experiences 10 times altogether
Hot housing.
Preferably, in whole strain, the mass percent concentration of the nicotine aqueous solution is followed successively by 1.5 ‰, 2.0 ‰,
2.5‰、3.0‰；Namely black refers to that gushing water horsefly ovum experiences 4 hot housing altogether.
The black of resistance to nicotine refers to that gushing water horsefly kind refers to the resistance to nicotine for referring to from wild black and being filtered out in gushing water horsefly kind
Black refers to gushing water horsefly kind.
It is described to refer to that the black of resistance to nicotine is filtered out in gushing water horsefly kind refers to that the specific method of gushing water horsefly kind is from wild black：
S1. configuration traps compost, will trap compost and is fitted into open containers；
It is described to trap each component that compost contains following percetage by weight：Wheat bran 70~90%, imported fish meal 10~30%,
It is 70% that above-mentioned formulation material is adjusted into humidity with the nicotine aqueous solution of mass percent concentration 1 ‰~2 ‰；
S2. when weather is fine, the morning（It is preferred that 8:00）Will be equipped with trapping compost container be put in respectively house refuse heap,
Chicken house and pig farm trap wild worm's ovum, afternoon（It is preferred that 17:00）Withdrawal is put in container everywhere；
S3. by reclaim each container be placed on indoor culture, wait the wild worm's ovum for trapping cultivate to larva it is aging when, utilize
Black refers to gushing water horsefly prepupa it is separated with formulation material from food phase behavior（Can voluntarily be climbed when i.e. black refers to that gushing water horsefly prepupa is aging
From compost, find dry place and pupate）；
S4. refer to the Morphological Identification of gushing water horsefly larva by black, choose black and refer to gushing water horsefly prepupa；
S5. filter out that 20~25 days aging, every prepupa weighs 0.10~0.16 g, the black that figure is plump, color is pitch-black
Refer to gushing water horsefly prepupa, at ambient temperature, allow black refer to gushing water horsefly prepupa pupate naturally, sprouts wings after by the resistance to nicotine of acquisition it is black
Color refers to gushing water horsefly kind.
Preferably, described indoor conditions is 25~30 DEG C of temperature, humidity 30~70%.
The collocation method of the initial incubation material is after each component of initial incubation material is mixed, to use mass percent concentration
It is 70% that above-mentioned formulation material is adjusted to humidity by the 1 ‰ nicotine aqueous solution.
Preferably, black is accessed in every 1 kg initial incubation material and refers to the g of gushing water horsefly ovum 0.5~1.0；Every 1 kg hot housing
Black is accessed in material and refers to the g of gushing water horsefly ovum 0.5~1.0.
Described its adult of nursing is fed using water.Preferably, the clear water may be selected pure water, running water, well water
Or nontoxic river etc.；The mode that water is fed is to be saturated with water using sponge, is sucked for adult.
The beneficial effects of the invention are as follows：
Proportion compatibility is determined by science, the present invention is creatively directly using the nicotine aqueous solution and wheat bran of suitable concentration
With imported fish meal scientific compatibility, the first formulation material of acquisition not only successfully covers the unpleasant and bitter taste of nicotine, and nutritional ingredient is appropriate,
And the reasonability configured before and after initial incubation material and nutriment contained by hot housing material and nicotine equal size, it is ensured that black refers to
Gushing water horsefly larval growth is smooth, and because the appropriateness and certainty of nicotine content, and initial incubation material and hot housing
The reasonability of configuration before and after the contained nutriment of material and nicotine equal size, it is ensured that in same nurturing an environment, different batches
The formulation material of same weight composition obtains the cultivate effect of stabilization.
By hot housing method of the invention, black both can be effectively stepped up and refer to tolerance of the gushing water horsefly kind to nicotine
Ability, is unlikely to refer to black gushing water horsefly kind causes serious diapause again, can by easy stages strengthen wild black and refer to gushing water
Horsefly kind to the tolerance of nicotine, until the nicotine aqueous solution in compost mass percent concentration up to untill 3 ‰.
Specific embodiment
The present invention is further described with reference to specific embodiment.Unless stated otherwise, the present invention use reagent,
Equipment is the art conventional reagent and equipment.
Nicotine is purchased from conventional chemical reagent shop, purity >=99.5%.
Embodiment 1 refers to that the black of resistance to nicotine is filtered out in gushing water horsefly kind refers to gushing water horsefly kind from wild black
S01. when weather is fine, preparation traps compost, each component for trapping compost by following percetage by weight
Composition：Wheat bran 90%, imported fish meal 10%, humidity is adjusted to the nicotine aqueous solution of mass percent concentration 1 ‰ by above-mentioned formulation material
It is 70%.
S02. by the above-mentioned compost for modulating, every 1 kg is fitted into a square plastic feed basin（* * wide long are high=45cm*30cm*
12cm）.In morning 8 on the same day:00 plastic tub that will be equipped with compost is put in by a life rubbish heap, afternoon 17:00 withdraws everywhere
Plastic tub, takes back indoor culture.
S03. in room conditions（25 DEG C of temperature, humidity 30%）, the wild worm's ovum that will be trapped cultivates aging to larva,
Refer to gushing water horsefly prepupa prepupa is separated with formulation material from food phase behavior using black（Meeting when i.e. black refers to that gushing water horsefly prepupa is aging
Voluntarily climb from compost, find dry place and pupate）.
S04. by the Morphological Identification of larva under anatomical lens, black is individually chosen with tweezers and refers to gushing water horsefly prepupa, arranged
Except other fly kinds.
S05. filter out that 20~25 days aging, every prepupa weighs 0.10~0.16 g, the black that figure is plump, color is pitch-black
Refer to gushing water horsefly prepupa, at ambient temperature, allow these black refer to gushing water horsefly prepupa pupate naturally, sprouts wings after by the resistance to nicotine of acquisition
Black refer to gushing water horsefly kind.
Embodiment 2 refers to that the black of resistance to nicotine is filtered out in gushing water horsefly kind refers to gushing water horsefly kind from wild black
S01. when weather is fine, preparation traps compost, each component for trapping compost by following percetage by weight
Composition：, be adjusted to for above-mentioned formulation material with the nicotine aqueous solution of mass percent concentration 1.5 ‰ wet by wheat bran 80%, imported fish meal 20%
Spend is 70%.
S02. by the above-mentioned compost for modulating, it is fitted into a square plastic feed basin per 1kg（* * wide long are high=45cm*30cm*
12cm）.In morning 8 on the same day:00 plastic tub that will be equipped with compost is put in by a life rubbish heap, afternoon 17:00 withdraws everywhere
Plastic tub, takes back indoor culture.
S03. in room conditions（25 DEG C of temperature, humidity 30%）, the wild worm's ovum that will be trapped cultivates aging to larva,
Refer to gushing water horsefly prepupa prepupa is separated with formulation material from food phase behavior using black（Meeting when i.e. black refers to that gushing water horsefly prepupa is aging
Voluntarily climb from compost, find dry place and pupate）.
S04. by the Morphological Identification of larva under anatomical lens, black is individually chosen with tweezers and refers to gushing water horsefly prepupa, arranged
Except other fly kinds.
S05. filter out that 20~25 days aging, every prepupa weighs 0.10~0.16 g, the black that figure is plump, color is pitch-black
Refer to gushing water horsefly prepupa, at ambient temperature, allow these black refer to gushing water horsefly prepupa pupate naturally, sprouts wings after by the resistance to nicotine of acquisition
Black refer to gushing water horsefly kind.
Embodiment 3 refers to that the black of resistance to nicotine is filtered out in gushing water horsefly kind refers to gushing water horsefly kind from wild black
S01. when weather is fine, preparation traps compost, each component for trapping compost by following percetage by weight
Composition：Wheat bran 70%, imported fish meal 30%, humidity is adjusted to the nicotine aqueous solution of mass percent concentration 2 ‰ by above-mentioned formulation material
It is 70%.
S02. by the above-mentioned compost for modulating, every 1 kg is fitted into a square plastic feed basin（* * wide long are high=45cm*30cm*
12cm）.In morning 8 on the same day:00 plastic tub that will be equipped with compost is put in by a life rubbish heap, afternoon 17:00 withdraws everywhere
Plastic tub, takes back indoor culture.
S03. in room conditions（25 DEG C of temperature, humidity 30%）, the wild worm's ovum that will be trapped cultivates aging to larva,
Refer to gushing water horsefly prepupa prepupa is separated with formulation material from food phase behavior using black（Meeting when i.e. black refers to that gushing water horsefly prepupa is aging
Voluntarily climb from compost, find dry place and pupate）.
S04. by the Morphological Identification of larva under anatomical lens, black is individually chosen with tweezers and refers to gushing water horsefly prepupa, arranged
Except other fly kinds.
S5. filter out that 20~25 days aging, every prepupa weighs 0.10~0.16 g, the black that figure is plump, color is pitch-black
Refer to gushing water horsefly prepupa, at ambient temperature, allow these black refer to gushing water horsefly prepupa pupate naturally, sprouts wings after by the resistance to nicotine of acquisition
Black refer to gushing water horsefly kind.
4 pairs of black of resistance to nicotine for screening of embodiment refer to that gushing water horsefly kind carries out hot housing and makes its tolerance quality hundred
Divide the formulation material of the nicotine aqueous solution that specific concentration is 3 ‰
The black of resistance to nicotine that any embodiment of embodiment 1~3 is screened is referred to that gushing water horsefly kind is cultivated, makes its resistance to
By the formulation material of the nicotine aqueous solution that mass percent concentration is 3 ‰, specific method is as follows：
S11. starting stage：
Initial incubation material is prepared, the initial incubation material is grouped into by each group of following percetage by weight：Wheat bran 90%, enter
Mouth fish meal 10%, it is 70% that above-mentioned formulation material is adjusted into humidity with the nicotine aqueous solution of mass percent concentration 1 ‰.By above-mentioned modulation
Good compost, every 1 kg is fitted into a square plastic feed basin（* * wide long are high=45cm*30cm*12cm）, per basin, access black refers to prominent
The g of stratiomyiid ovum 0.8；In room conditions（25~30 DEG C of temperature, humidity 30~70%）, by above-mentioned worm's ovum cultivate to larva it is aging,
After pupating, sprouting wings, its adult is fed（Feeding method：According to percent by weight, the whole milk powder, the usage ratio of brown sugar
It is whole milk powder 70%, brown sugar 30%；The mode that water is fed is to be saturated with water using sponge, is sucked for adult.Similarly hereinafter）.
Strain：
S12. the 1st strain：After the ovum for after the Adult worms producting eggs of starting stage, being produced using the 1st reinforcing training
Nutriment carries out hot housing；The 1st hot housing material contains each component of following percetage by weight：Wheat bran 80%, import fish
Powder 20%, uses mass percent concentration（1+S）Above-mentioned formulation material is adjusted to humidity for 70%, S is selected from 0.1 by the ‰ nicotine aqueous solution
~0.5；In room conditions（25~30 DEG C of temperature, humidity 30~70%）, by above-mentioned worm's ovum cultivate to larva it is aging, pupate, plumage
After change, its adult is fed.
S13. the N+1 times strain：Hot housing is carried out again to the ovum that the adult obtained by n-th strain produces,
And component contained by the compost used by the N+1 times strain meets following ratio：The mass percent of the nicotine aqueous solution is dense
Degree=（N-th cultivates the content+S of nicotine in compost）‰, remaining constituent content is constant, and N is the natural number more than or equal to 1, weight
Multiple reinforcing, until the nicotine aqueous solution in compost described in last time strain mass percent concentration up to untill 3 ‰.
Preferably, in this implementation, in whole breeding phase, the mass percent concentration of the nicotine aqueous solution is followed successively by
1.1‰、1.2‰、1.3‰、1.4‰、1.5‰、1.6‰、1.7‰、1.8‰、1.9‰、2.0‰、2.1‰、2.2‰、
2.3‰、2.4‰、2.5‰、2.6‰、2.7‰、2.8‰、2.9‰、3.0‰；Namely black refers to that gushing water horsefly ovum experiences 20 times altogether
Hot housing.The black that this stage turns out refers to that gushing water horsefly kind has tolerance higher to nicotine.
5 pairs of black of resistance to nicotine for screening of embodiment refer to that gushing water horsefly kind carries out hot housing and makes its tolerance quality hundred
Divide the formulation material of the nicotine aqueous solution that specific concentration is 3 ‰
The black of resistance to nicotine that any embodiment of embodiment 1~3 is screened is referred to that gushing water horsefly kind is cultivated, makes its resistance to
By the formulation material of the nicotine aqueous solution that mass percent concentration is 3 ‰, specific method is as follows：
S11. starting stage：
Initial incubation material is prepared, the initial incubation material is grouped into by each group of following percetage by weight：Wheat bran 80%, enter
Mouth fish meal 20%, it is 70% that above-mentioned formulation material is adjusted into humidity with the nicotine aqueous solution of mass percent concentration 1 ‰.By above-mentioned modulation
Good compost, every 1 kg is fitted into a square plastic feed basin（* * wide long are high=45cm*30cm*12cm）, per basin, access black refers to prominent
The g of stratiomyiid ovum 0.8；In room conditions（25~30 DEG C of temperature, humidity 30~70%）, by above-mentioned worm's ovum cultivate to larva it is aging,
After pupating, sprouting wings, its adult is fed.
Strain：
S12. the 1st strain：After the ovum for after the Adult worms producting eggs of starting stage, being produced using the 1st reinforcing training
Nutriment carries out hot housing；The 1st hot housing material contains each component of following percetage by weight：Wheat bran 80%, import fish
Powder 20%, uses mass percent concentration（1+S）Above-mentioned formulation material is adjusted to humidity for 70%, S is selected from 0.1 by the ‰ nicotine aqueous solution
~0.5；In room conditions（25~30 DEG C of temperature, humidity 30~70%）, by above-mentioned worm's ovum cultivate to larva it is aging, pupate, plumage
After change, its adult is fed；
S13. the N+1 times strain：Hot housing is carried out again to the ovum that the adult obtained by n-th strain produces,
And component contained by the compost used by the N+1 times strain meets following ratio：The mass percent of the nicotine aqueous solution is dense
Degree=（N-th cultivates the content+S of nicotine in compost）‰, remaining constituent content is constant, and N is the natural number more than or equal to 1, weight
Multiple reinforcing, until the nicotine aqueous solution in compost described in last time strain mass percent concentration up to untill 3 ‰.
Preferably, in this implementation, in whole breeding phase, the mass percent concentration of the nicotine aqueous solution is followed successively by
1.2‰、1.4‰、1.6‰、1.8‰、2.0‰、2.2‰、2.4‰、2.6‰、2.8‰、3.0‰；Namely black refers to gushing water horsefly
Ovum experiences 10 hot housing altogether.The black that this stage turns out refers to that gushing water horsefly kind has tolerance higher to nicotine.
6 pairs of black of resistance to nicotine for screening of embodiment refer to that gushing water horsefly kind carries out hot housing and makes its tolerance quality hundred
Divide the formulation material of the nicotine aqueous solution that specific concentration is 3 ‰
The black of resistance to nicotine that any embodiment of embodiment 1~3 is screened is referred to that gushing water horsefly kind is cultivated, makes its resistance to
By the formulation material of the nicotine aqueous solution that mass percent concentration is 3 ‰, specific method is as follows：
S11. starting stage：
Initial incubation material is prepared, the initial incubation material is grouped into by each group of following percetage by weight：Wheat bran 70%, enter
Mouth fish meal 30%, it is 70% that above-mentioned formulation material is adjusted into humidity with the nicotine aqueous solution of mass percent concentration 1 ‰.By above-mentioned modulation
Good compost, every 1 kg is fitted into a square plastic feed basin（* * wide long are high=45cm*30cm*12cm）, per basin, access black refers to prominent
The g of stratiomyiid ovum 0.8；In room conditions（25~30 DEG C of temperature, humidity 30~70%）, by above-mentioned worm's ovum cultivate to larva it is aging,
After pupating, sprouting wings, its adult is fed.
Strain：
S12. the 1st strain：After the ovum for after the Adult worms producting eggs of starting stage, being produced using the 1st reinforcing training
Nutriment carries out hot housing；The 1st hot housing material contains each component of following percetage by weight：Wheat bran 80%, import fish
Powder 20%, uses mass percent concentration（1+S）Above-mentioned formulation material is adjusted to humidity for 70%, S is selected from 0.1 by the ‰ nicotine aqueous solution
~0.5；In room conditions（25~30 DEG C of temperature, humidity 30~70%）, by above-mentioned worm's ovum cultivate to larva it is aging, pupate, plumage
After change, its adult is fed；
S13. the N+1 times strain：Hot housing is carried out again to the ovum that the adult obtained by n-th strain produces,
And component contained by the compost used by the N+1 times strain meets following ratio：The mass percent of the nicotine aqueous solution is dense
Degree=（N-th cultivates the content+S of nicotine in compost）‰, remaining constituent content is constant, and N is the natural number more than or equal to 1, weight
Multiple reinforcing, until the nicotine aqueous solution in compost described in last time strain mass percent concentration up to untill 3 ‰.
Preferably, in this implementation, in whole breeding phase, the mass percent concentration of the nicotine aqueous solution is followed successively by
1.5‰、2.0‰、2.5‰、3.0‰；Namely black refers to that gushing water horsefly ovum experiences 4 hot housing altogether.It is black that this stage turns out
Color refers to that gushing water horsefly kind has tolerance higher to nicotine.
Comparative example 1
Operation with embodiment 1, unlike described trap compost and be grouped into by each group of following percetage by weight：Wheat bran
80% and imported fish meal 20%.Experiment is found using as above trapping the kind that the ovum that compost traps is not resistance to nicotine.
Embodiment 7
The black that the embodiment of the present invention 1 to 3 is screened is referred to that gushing water horsefly kind, embodiment 4 to 6 are tolerated by culture
The black of 3 ‰ nicotine formulation material refers to that the black after gushing water horsefly kind, the treatment of comparative example 1 refers to that gushing water horsefly kind carries out the energy of resistance to nicotine
Power is tested, and test process is：
The black after the embodiment culture treatment is taken respectively and refers to the ovum 0.5g that gushing water horsefly adult is produced, be respectively connected to 1kg
The formulation material of the nicotine aqueous solution of percent concentration containing better quality of weight（Wheat bran 80%, imported fish meal 20% are dense with mass percent
It is 70% spend the 3.0 ‰ nicotine aqueous solution above-mentioned formulation material to be adjusted into humidity）In, test result is shown in Table 1：
Table 1
| Treatment | Larvae development goes through the phase（My god） | Larva bar number/g（Bar） | Survival rate of larvae（%） |
| Embodiment 1 is initially planted | 28 | 14 | 64 |
| Embodiment 2 is initially planted | 28 | 12 | 68 |
| Embodiment 3 is initially planted | 28 | 13 | 65 |
| Comparative example 1 | 35 | 22 | 14 |
| Embodiment semi-finals kind | 21 | 8 | 96 |
| The reinforcing kind of embodiment 5 | 23 | 9 | 95 |
| The reinforcing kind of embodiment 6 | 24 | 10 | 94 |
Test result illustrates that comparative example 1 is not resistant to the nicotine aqueous solution of mass percent concentration 3.0 ‰, and black refers to prominent
Stratiomyiid larva can not enter prepupa state.
1 to 3 black by screening step of embodiment refers to nicotine water of the gushing water horsefly kind in mass percent concentration 3.0 ‰
Diapause phenomenon is there occurs in the formulation material of solution modulation, pre- pupal weight is lighter, and has relatively large dead larvae.The embodiment of the present invention
4 to 6 refer to that the resistance to nicotine ability of gushing water horsefly kind is greatly improved through the black after hot housing treatment, can well tolerate quality
The formulation material of the nicotine aqueous solution modulation of percent concentration 3.0 ‰ simultaneously obtains stable effect.
Claims (6)
1. a kind of black of resistance to nicotine refers to the intensifying method of gushing water horsefly kind, it is characterised in that comprise the following steps：
Starting stage：The ovum that the black of resistance to nicotine refers to gushing water horsefly kind is seeded in initial incubation material, the initial incubation material by
The component composition of following weight fraction：Wheat bran 70~90%, imported fish meal 10~30%, with mass percent concentration 1 ‰
It is 70% that the initial incubation material is adjusted to humidity by the nicotine aqueous solution；In room conditions, by the ovum cultivate to larva it is aging,
After pupating, sprouting wings, its adult is fed；
1st strain：Carried out by force using the 1st hot housing material after the ovum for after the Adult worms producting eggs of starting stage, being produced
Change culture；The 1st hot housing material is grouped into by each group of following weight fraction：Wheat bran 70~90%, imported fish meal
10~30%, the 1st hot housing material is adjusted into humidity with the nicotine aqueous solution of mass percent concentration (1+S) ‰ is
70%, S are selected from 0.1~0.5；In room conditions, by the ovum cultivate to larva it is aging, pupate, sprout wings after, feed its into
Worm；
The N+1 times strain：Hot housing, and N+1 are carried out again to the ovum that the adult obtained by n-th strain produces
Component contained by compost used by secondary strain meets following ratio：The mass percent concentration of the nicotine aqueous solution=(n-th
Content+the S of nicotine in hot housing material) ‰, remaining constituent content is constant, and N is the natural number more than or equal to 1, repeats to strengthen, directly
To the mass percent concentration of the nicotine aqueous solution in the compost used by last time strain up to 3 ‰；
The black of resistance to nicotine refers to that the screening technique of gushing water horsefly kind is：
S1. prepare and trap compost, compost will be trapped and be fitted into open containers；
The compost that traps is grouped into by each group of following weight fraction：Wheat bran 70~90%, imported fish meal 10~30%,
With the nicotine aqueous solution of mass percent concentration 1 ‰~2 ‰, trap compost to be adjusted to humidity be 70% by described；
S2. when weather is fine, the container that will be equipped with trapping compost in the morning is put in house refuse heap, chicken house and pig farm to lure respectively
Collect wild worm's ovum, withdraw the container being put in everywhere afternoon；
S3. by reclaim each container be placed on indoor culture, wait the wild worm's ovum for trapping cultivate to larva it is aging when, using black
Refer to gushing water horsefly prepupa prepupa is separated with compost is trapped from food phase behavior；
S4. refer to the Morphological Identification of gushing water horsefly larva by black, choose black and refer to gushing water horsefly prepupa；
S5. filter out that 20~25 days aging, every prepupa weighs 0.10~0.16g, the black that figure is plump, color is pitch-black refers to gushing water
Horsefly prepupa, at ambient temperature, allow black refer to gushing water horsefly prepupa pupate naturally, sprout wings after by obtain resistance to nicotine black refer to it is prominent
Stratiomyiid kind.
2. intensifying method according to claim 1, it is characterised in that the indoor conditions is 25~30 DEG C of temperature, humidity 30
~70%.
3. intensifying method according to claim 1, it is characterised in that the collocation method of the initial incubation material is will initial training
After each component of nutriment is mixed, add the nicotine aqueous solution of certain concentration that the humidity of initial incubation material is adjusted into 70%.
4. intensifying method according to claim 1, it is characterised in that access black in per 1kg initial incubation material and refer to gushing water horsefly
0.5~1.0g of ovum；Black is accessed in per 1kg hot housing material refer to 0.5~1.0g of gushing water horsefly ovum.
5. intensifying method according to claim 1, it is characterised in that the nursing its adult is fed using water.
6. intensifying method according to claim 5, it is characterised in that the water is pure water, running water, well water or nontoxic
River；The mode fed using water is to be saturated with water using sponge, is sucked for adult.
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