CN104285895B - A kind of intensifying method of the lucilia sericata of resistance to nicotine kind - Google Patents
A kind of intensifying method of the lucilia sericata of resistance to nicotine kind Download PDFInfo
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- CN104285895B CN104285895B CN201410421759.7A CN201410421759A CN104285895B CN 104285895 B CN104285895 B CN 104285895B CN 201410421759 A CN201410421759 A CN 201410421759A CN 104285895 B CN104285895 B CN 104285895B
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- SNICXCGAKADSCV-JTQLQIEISA-N (-)-Nicotine Chemical compound CN1CCC[C@H]1C1=CC=CN=C1 SNICXCGAKADSCV-JTQLQIEISA-N 0.000 title claims abstract description 130
- 229960002715 nicotine Drugs 0.000 title claims abstract description 130
- SNICXCGAKADSCV-UHFFFAOYSA-N nicotine Natural products CN1CCCC1C1=CC=CN=C1 SNICXCGAKADSCV-UHFFFAOYSA-N 0.000 title claims abstract description 130
- 241000736227 Lucilia sericata Species 0.000 title claims abstract description 99
- 238000000034 method Methods 0.000 title claims abstract description 33
- 239000000463 material Substances 0.000 claims abstract description 71
- 239000007864 aqueous solution Substances 0.000 claims abstract description 50
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 33
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 33
- 210000004681 ovum Anatomy 0.000 claims abstract description 33
- 235000019733 Fish meal Nutrition 0.000 claims abstract description 21
- 239000004467 fishmeal Substances 0.000 claims abstract description 21
- 238000011534 incubation Methods 0.000 claims abstract description 20
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 20
- 239000002361 compost Substances 0.000 claims description 52
- 230000032683 aging Effects 0.000 claims description 26
- 238000005728 strengthening Methods 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 239000000843 powder Substances 0.000 claims description 7
- 235000013601 eggs Nutrition 0.000 claims description 6
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical group C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 claims description 5
- 239000000470 constituent Substances 0.000 claims description 5
- 235000013305 food Nutrition 0.000 claims description 5
- 230000000877 morphologic effect Effects 0.000 claims description 5
- 235000013336 milk Nutrition 0.000 claims description 4
- 239000008267 milk Substances 0.000 claims description 4
- 210000004080 milk Anatomy 0.000 claims description 4
- 238000012216 screening Methods 0.000 claims description 4
- 235000008939 whole milk Nutrition 0.000 claims description 4
- 241000287828 Gallus gallus Species 0.000 claims description 3
- 230000000474 nursing effect Effects 0.000 claims description 2
- 229920006395 saturated elastomer Polymers 0.000 claims description 2
- 241000628997 Flos Species 0.000 claims 1
- 241000257166 Lucilia cuprina Species 0.000 claims 1
- 150000001875 compounds Chemical class 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 7
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 34
- 239000000203 mixture Substances 0.000 description 33
- 238000009472 formulation Methods 0.000 description 32
- 241000208125 Nicotiana Species 0.000 description 22
- 244000061176 Nicotiana tabacum Species 0.000 description 12
- 230000008569 process Effects 0.000 description 11
- 241000257159 Musca domestica Species 0.000 description 7
- 230000000052 comparative effect Effects 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 6
- 235000019504 cigarettes Nutrition 0.000 description 5
- 241000238631 Hexapoda Species 0.000 description 4
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 4
- 238000009825 accumulation Methods 0.000 description 4
- 230000006399 behavior Effects 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 238000005520 cutting process Methods 0.000 description 3
- 230000007423 decrease Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000002917 insecticide Substances 0.000 description 3
- 239000010813 municipal solid waste Substances 0.000 description 3
- 244000046052 Phaseolus vulgaris Species 0.000 description 2
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 2
- 235000013877 carbamide Nutrition 0.000 description 2
- 239000004202 carbamide Substances 0.000 description 2
- 230000005058 diapause Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 210000003608 fece Anatomy 0.000 description 2
- 230000004720 fertilization Effects 0.000 description 2
- 239000003337 fertilizer Substances 0.000 description 2
- 239000010871 livestock manure Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000010815 organic waste Substances 0.000 description 2
- 230000000704 physical effect Effects 0.000 description 2
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 description 1
- 206010058314 Dysplasia Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- FHKPLLOSJHHKNU-INIZCTEOSA-N [(3S)-3-[8-(1-ethyl-5-methylpyrazol-4-yl)-9-methylpurin-6-yl]oxypyrrolidin-1-yl]-(oxan-4-yl)methanone Chemical compound C(C)N1N=CC(=C1C)C=1N(C2=NC=NC(=C2N=1)O[C@@H]1CN(CC1)C(=O)C1CCOCC1)C FHKPLLOSJHHKNU-INIZCTEOSA-N 0.000 description 1
- 230000009418 agronomic effect Effects 0.000 description 1
- 230000003466 anti-cipated effect Effects 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 235000013405 beer Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 235000019658 bitter taste Nutrition 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000010794 food waste Substances 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 230000001418 larval effect Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000618 nitrogen fertilizer Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 235000010333 potassium nitrate Nutrition 0.000 description 1
- 239000004323 potassium nitrate Substances 0.000 description 1
- 239000002516 radical scavenger Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 239000008399 tap water Substances 0.000 description 1
- 235000020679 tap water Nutrition 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Life Sciences & Earth Sciences (AREA)
- Polymers & Plastics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Behavior & Ethology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Insects & Arthropods (AREA)
- Birds (AREA)
- Marine Sciences & Fisheries (AREA)
- Biomedical Technology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses the intensifying method of a kind of lucilia sericata of resistance to nicotine kind.The present invention utilizes initial incubation material to cultivate the wild lucilia sericata of the resistance to nicotine kind ovum screened to adult, the ovum of adult output utilize hot housing material carry out n times hot housing to adult, in hot housing circulation, the mass percent concentration of nicotine aqueous solution all can improve 0.1～0.5 point, until the mass percent concentration of nicotine aqueous solution reaches 3 ‰ on the basis of upper once hot housing material every time.The present invention directly uses nicotine aqueous solution and wheat bran and the imported fish meal scientific compatibility of suitable concentration, success hot housing lucilia sericata, can strengthen the wild lucilia sericata kind tolerance to nicotine by easy stages, it is thus achieved that more stable cultivation effect.
Description
Technical field
The present invention relates to animal varieties triage techniques field, be specifically related to the intensifying method of a kind of lucilia sericata of resistance to nicotine kind.
Background technology
Inapplicable fresh tobacco leaf refers to not to be suitable for the fresh tobacco leaves (water content 80～90%) of cured tobacco production, different from waste/hypo-tobacco leaf or the discarded tobacco leaf (water content about 15%) produced in the production process of flue-cured tobacco mentioned in a large number in document.Inapplicable Nicotiana tabacum L. typically refers to after cigarette strain normally binds and remove 2～3 milk foot leaves, cigarette industry is inapplicable, need to process in field removing inferior leads and upper leaf, mainly include bottom cigarette strain because of illumination deficiency, dysplasia, inclusions is not enough, anticipated roasting after inferior quality, do not possess 2 inferior leads that baking is worth, and cigarette strain top hack bad, curtailment, structure are stiff, roasting after 2 tops of inferior quality.
If these inapplicable Nicotiana tabacum Lves arbitrarily abandon field, easily cause the repeated infection of environmental pollution and tobacco virus, the processing mode destroyed or fill of concentrating according to prior art then needs to spend substantial amounts of expense, the processing cost of industry inapplicable Nicotiana tabacum L. per ton averagely up to 200 yuan at present, each Nicotiana tabacum L. producing region generally requires under the organization leadership of government of local party committee, carry out policy propaganda, tabulation registration, build treatment facility, unified remove, record archive, publicity, cash the measures such as subsidy and supervise the process carrying out inapplicable Nicotiana tabacum L., waste time and energy costly.
It is a kind of preferably alternate process method, environmental protection, high financial profit that the insecticide using resistance to nicotine carries out biological treatment to inapplicable Nicotiana tabacum L..In order to utilize insecticide to realize inapplicable fresh tobacco leaf of carrying out a biological disposal upon, first need to filter out the insect species of resistance to nicotine.
The applicant studies for a long period of time based on to housefly and inapplicable fresh tobacco leaf treatment technology, sums up and utilizes the screening that inapplicable fresh tobacco leaf carries out resistance to nicotine strain to housefly, the method strengthened and cultivate.Described strengthening is the ovum of the resistance to nicotine housefly filtered out to be inoculated in inapplicable fresh tobacco leaf compost cultivate to adult, the ovum of adult output utilize hot housing material carry out n times hot housing again to adult, every time in hot housing circulation, inapplicable fresh tobacco leaf accounts for the ratio of compost and all can improve 5～15 points on the basis of upper once hot housing material, until the ratio that inapplicable fresh tobacco leaf is in hot housing material reaches 85%, the housefly kind that this stage turns out i.e. has higher tolerance to nicotine.Described inapplicable fresh tobacco leaf compost contains each component of following percetage by weight: inapplicable fresh tobacco leaf 30%, wheat bran 60%, imported fish meal 5%, bean cake 5%.
This technical scheme can go out the housefly kind of resistance to nicotine with successful incubation, but the resistance to nicotine ability that different batches cultivates the aging fly larvae obtained is the most otherwise varied, there is the uncertainty of effect.Main reason is that the application aspect of inapplicable Nicotiana tabacum L., formulation material adds the inapplicable Nicotiana tabacum L. of zone of reasonableness can reduce the nicotine toxic action to Musca domestica larva although summing up through creative research and great many of experiments, but the factor affecting inapplicable fresh tobacco leaf nicotine content is the most various, complicated mechanism, it is more difficult to realize accurately controlling.The factor affecting nicotine content in tobacco leaf specifically includes that (1) different growing stages, in Nicotiana tabacum L., contained nicotine content is different: in tobacco seed, nicotine content is 0, seedling raising process rises beginning formed at root, after developing along with root system, synthesis and the accumulation ability of nicotine gradually strengthen, so different growing stages, in Nicotiana tabacum L., contained nicotine content is different；(2) cigarette strain different parts, nicotine content is dramatically different, and nicotine content is also by leaf position and number of sheets large effect；(3) nicotine amount contained by different cultivars Nicotiana tabacum L. is different；(4) weather conditions etc. of soil dry-wet all have the impact of different savings to nicotine content；(5) the different appreciable impact nicotine content of tobacco leaves of agronomic measures, including fertilization type, planting density, pinch and smear wooden fork fertilising, girdling and the nicotine content cut in root fertilizer, picking methods etc. factor all appreciable impact Nicotiana tabacum Lves, as a example by fertilization type, in the nitrogenous fertilizer of three kinds of forms, after using carbamide, nicotine accumulation is the highest, and ammonium nitrate takes second place, the nicotine accumulation that potassium nitrate processes is minimum, lower by 27% than using carbamide；As a example by Physical property, Zhang Dan etc. studies and finds, postpones nicotine accumulation intensity peak with Physical property and declines successively, and nicotine content lowers the most successively, often postpones and pinching for one day, nicotine content just decline 0.0504%；Again as a example by cutting root fertilizer, cutting root 1/8 compared with not cutting root, upper leaf nicotine declines 1.9 percentage points of ranges of decrease 31.33%, and middle leaf nicotine declines 2.13 percentage points, the range of decrease 51.4%.
The uncertainty how avoiding employing different batches compost to cultivate the aging fly larvae effect obtained is the technical barrier that this area is urgently to be resolved hurrily.And use and stablize feasible technical scheme and cultivate the insect strain of resistance to nicotine of more multi items and carry out reinforced cultivating effectively, it is this area simultaneously need to the important technological problems that solves.
Summary of the invention
The technical problem to be solved in the present invention is to overcome the defect lacking the strong lucilia sericata of the resistance to nicotine kind of the tolerance to nicotine in prior art, it is provided that the intensifying method of a kind of lucilia sericata of resistance to nicotine kind.
The purpose of the present invention is achieved by the following technical programs:
Lucilia sericata is different from the another kind of insecticide of housefly, and it is a kind of important economic insects and scavenger insect, and available lucilia sericata larvae processes various corrupt organic waste, such as beer residue, bean dregs, chicken manure, pig manure and changing food waste etc..Existing lucilia sericata kind all has preferable biological treatment capabilities to nontoxic organic waste, but the best to the treatment effect of inapplicable fresh tobacco leaf.Main cause is because in inapplicable fresh tobacco leaf, containing toxicants such as a certain amount of nicotine, having the effect of certain murder by poisoning and Developing restraint to lucilia sericata larvae (lucilia sericata maggot).In order to utilize the lucilia sericata inapplicable fresh tobacco leaf of biological treatment, first need to filter out the lucilia sericata kind of resistance to nicotine from wild lucilia sericata kind, improve the lucilia sericata tolerance to fresh tobacco leaf.
The present invention provides the intensifying method of a kind of lucilia sericata of resistance to nicotine kind, comprises the steps:
Starting stage: the ovum of the lucilia sericata of resistance to nicotine kind is seeded in initial incubation material, described initial incubation material contains each component of following percetage by weight: wheat bran 70～90%, imported fish meal 10～30%, with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 60%；In room conditions, above-mentioned fly blow is cultivated aging to larva, pupate, sprout wings after, utilize milk powder, brown sugar and water to feed its adult；
1st strain: after the Adult worms producting eggs of starting stage, the ovum produced uses the 1st hot housing material to carry out hot housing；Described 1st hot housing material contains each component of following percetage by weight: wheat bran 80%, imported fish meal 20%, with the nicotine aqueous solution of mass percent concentration (1+S) ‰, above-mentioned formulation material being adjusted to humidity is 60%, and S is selected from 0.1～0.5；In room conditions, above-mentioned fly blow is cultivated aging to larva, pupate, sprout wings after, feed its adult；
The N+1 time strain: the ovum of the adult product of n-th strain gained is carried out hot housing again, and component contained by the compost used by the N+1 time strain meets following ratio: the mass percent concentration of nicotine aqueous solution=(content+S of nicotine in n-th hot housing material) ‰, remaining constituent content is constant, N is the natural number more than or equal to 1, repeat strengthening, till the mass percent concentration of nicotine aqueous solution reaches 3 ‰ in compost described in last strain.The lucilia sericata kind that this stage turns out i.e. has higher tolerance to nicotine.
Preferably, in whole strain, the mass percent concentration of nicotine aqueous solution is followed successively by 1.1 ‰, 1.2 ‰, 1.3 ‰, 1.4 ‰, 1.5 ‰, 1.6 ‰, 1.7 ‰, 1.8 ‰, 1.9 ‰, 2.0 ‰, 2.1 ‰, 2.2 ‰, 2.3 ‰, 2.4 ‰, 2.5 ‰, 2.6 ‰, 2.7 ‰, 2.8 ‰, 2.9 ‰, 3.0 ‰；Namely lucilia sericata ovum experiences 20 hot housing altogether.
Preferably, in whole strain, the mass percent concentration of nicotine aqueous solution is followed successively by 1.2 ‰, 1.4 ‰, 1.6 ‰, 1.8 ‰, 2.0 ‰, 2.2 ‰, 2.4 ‰, 2.6 ‰, 2.8 ‰, 3.0 ‰；Namely lucilia sericata ovum experiences 10 hot housing altogether.
Preferably, in whole strain, the mass percent concentration of nicotine aqueous solution is followed successively by 1.5 ‰, 2.0 ‰, 2.5 ‰, 3.0 ‰；Namely lucilia sericata ovum experiences 4 hot housing altogether.
The described lucilia sericata of resistance to nicotine kind refers to the lucilia sericata of the resistance to nicotine kind filtered out from wild lucilia sericata kind.
Described from wild lucilia sericata kind, filter out the lucilia sericata of resistance to nicotine kind method particularly includes:
S1. configuration lures collection compost, and collection compost will be lured to load in open containers；
The described collection compost each component that contains following percetage by weight that lures: wheat bran 70～90%, imported fish meal 10～30%, with the nicotine aqueous solution of mass percent concentration 1 ‰～2 ‰, above-mentioned formulation material being adjusted to humidity is 60%；
S2., when weather is fine, will be equipped with luring the container of collection compost to be put in house refuse heap, chicken house and pig farm respectively the morning (preferably 8:00) and lure the wild fly blow of collection, regain the container being put in everywhere afternoon (preferably 17:00)；
S3. each container reclaimed is placed on indoor cultivation, time aging etc. the wild fly blow cultivation luring collection to arrive to larva, utilize lucilia sericata maggot separates (can climb voluntarily when i.e. lucilia sericata maggot is aging from compost, find the place being dried and pupate) from food phase behavior by it with formulation material；
S4. by the Morphological Identification of lucilia sericata larvae, lucilia sericata maggot is chosen；
S5. filter out 4～5 days lucilia sericata maggots aging, that every fly larvae weighs 0.03～0.05g, figure is plump, color is golden yellow, at ambient temperature, allow lucilia sericata maggot naturally pupate, sprout wings after can obtain the lucilia sericata kind of resistance to nicotine.
Preferably, described indoor conditions is temperature 25～30 DEG C, humidity 30～70%.
The collocation method of described initial incubation material is that with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 60% by after each component mixing of initial incubation material.
Preferably, every 1 kg initial incubation material accesses lucilia sericata ovum 0.5～1.0 g；Every 1 kg hot housing material accesses lucilia sericata ovum 0.5～1.0 g.
Its adult of described nursing is all to utilize whole milk powder, brown sugar and water to feed.Preferably, according to percent by weight, described whole milk powder, the usage ratio of brown sugar are whole milk powder 70%, brown sugar 30%；The mode that water is fed is to utilize sponge to be saturated with water, sucks for adult, can use tap water or the pure water of cleaning.
The invention has the beneficial effects as follows:
Proportion compatibility is determined by science, the present invention the most directly uses nicotine aqueous solution and wheat bran and the imported fish meal scientific compatibility of suitable concentration, the first formulation material obtained the most successfully covers the unpleasant of nicotine and bitterness, nutritional labeling is suitable, ensure lucilia sericata larvae growth smoothly, and because the appropriateness of nicotine content and definitiveness, and the reasonability of configuration before and after nutrient substance contained by initial incubation material and hot housing material and nicotine equal size, guarantee in same nurturing an environment, the formulation material of the same weight composition of different batches obtains stable cultivation effect.
By the hot housing method of the present invention, both the lucilia sericata kind tolerance to nicotine can effectively have been stepped up, it is unlikely to again lucilia sericata kind is caused serious diapause, the wild lucilia sericata kind tolerance to nicotine can be strengthened by easy stages, till the mass percent concentration of nicotine aqueous solution reaches 3 ‰ in compost.
Detailed description of the invention
The present invention is further described below in conjunction with specific embodiment.Unless stated otherwise, the present invention uses reagent, equipment are the art conventional reagent and equipment.
Nicotine is purchased from conventional chemical reagent shop, purity >=99.5%.
Embodiment 1The lucilia sericata of resistance to nicotine kind is filtered out from wild lucilia sericata kind
S01. when weather is fine, preparation lure collection compost, described in lure collection compost be made up of each component of following percetage by weight: wheat bran 90%, imported fish meal 10%, with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 60%.
S02. by the above-mentioned compost modulated, every 1 kg loads (long * width * height=45cm*30cm*12cm) in a square plastic feed basin.In the morning on the same day 8:00 will be equipped with the plastic tub of compost and be put in by a life rubbish heap, afternoon, 17:00 regained plastic tub everywhere, took back indoor cultivation.
(temperature 25 DEG C the most in room conditions, humidity 30%), cultivating aging to larva by the wild fly blow luring collection to arrive, utilize lucilia sericata maggot separates (can climb voluntarily when i.e. lucilia sericata maggot is aging from compost, find the place being dried and pupate) from food phase behavior by fly larvae with formulation material.
S04. by the Morphological Identification of larva under anatomical lens, individually choose lucilia sericata maggot with tweezers, get rid of other fly kinds.
S05. filter out 4～5 days lucilia sericata maggots aging, that every fly larvae weighs 0.03～0.05 g, figure is plump, color is golden yellow, at ambient temperature, allow these lucilia sericata maggots naturally pupate, sprout wings after can obtain the lucilia sericata kind of resistance to nicotine.
Embodiment 2The lucilia sericata of resistance to nicotine kind is filtered out from wild lucilia sericata kind
S01. when weather is fine, preparation lure collection compost, described in lure collection compost be made up of each component of following percetage by weight: wheat bran 80%, imported fish meal 20%, with the nicotine aqueous solution of mass percent concentration 1.5 ‰, above-mentioned formulation material being adjusted to humidity is 60%.
S02. by the above-mentioned compost modulated, every 1kg loads (long * width * height=45cm*30cm*12cm) in a square plastic feed basin.In the morning on the same day 8:00 will be equipped with the plastic tub of compost and be put in by a life rubbish heap, afternoon, 17:00 regained plastic tub everywhere, took back indoor cultivation.
(temperature 25 DEG C the most in room conditions, humidity 30%), cultivating aging to larva by the wild fly blow luring collection to arrive, utilize lucilia sericata maggot separates (can climb voluntarily when i.e. lucilia sericata maggot is aging from compost, find the place being dried and pupate) from food phase behavior by fly larvae with formulation material.
S04. by the Morphological Identification of larva under anatomical lens, individually choose lucilia sericata maggot with tweezers, get rid of other fly kinds.
S05. filter out 4～5 days lucilia sericata maggots aging, that every fly larvae weighs 0.03～0.05 g, figure is plump, color is golden yellow, at ambient temperature, allow these lucilia sericata maggots naturally pupate, sprout wings after can obtain the lucilia sericata kind of resistance to nicotine.
Embodiment 3The lucilia sericata of resistance to nicotine kind is filtered out from wild lucilia sericata kind
S01. when weather is fine, preparation lure collection compost, described in lure collection compost be made up of each component of following percetage by weight: wheat bran 70%, imported fish meal 30%, with the nicotine aqueous solution of mass percent concentration 2 ‰, above-mentioned formulation material being adjusted to humidity is 60%.
S02. by the above-mentioned compost modulated, every 1 kg loads (long * width * height=45cm*30cm*12cm) in a square plastic feed basin.In the morning on the same day 8:00 will be equipped with the plastic tub of compost and be put in by a life rubbish heap, afternoon, 17:00 regained plastic tub everywhere, took back indoor cultivation.
(temperature 25 DEG C the most in room conditions, humidity 30%), cultivating aging to larva by the wild fly blow luring collection to arrive, utilize lucilia sericata maggot separates (can climb voluntarily when i.e. lucilia sericata maggot is aging from compost, find the place being dried and pupate) from food phase behavior by fly larvae with formulation material.
S04. by the Morphological Identification of larva under anatomical lens, individually choose lucilia sericata maggot with tweezers, get rid of other fly kinds.
S5. filter out 4～5 days lucilia sericata maggots aging, that every fly larvae weighs 0.03～0.05 g, figure is plump, color is golden yellow, at ambient temperature, allow these lucilia sericata maggots naturally pupate, sprout wings after can obtain the lucilia sericata kind of resistance to nicotine.
Embodiment 4 The lucilia sericata of the resistance to nicotine kind screened is carried out the formulation material of hot housing make it tolerate nicotine aqueous solution that mass percent concentration is 3 ‰
The lucilia sericata of the resistance to nicotine kind that embodiment 1～3 any embodiment screens is cultivated so that it is tolerance mass percent concentration is the formulation material of the nicotine aqueous solution of 3 ‰, and concrete grammar is as follows:
S11. the starting stage:
Preparation initial incubation material, described initial incubation material is made up of each component of following percetage by weight: wheat bran 90%, imported fish meal 10%, and with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 60%.By the above-mentioned compost modulated, every 1 kg loads (long * width * height=45cm*30cm*12cm) in a square plastic feed basin, and every basin accesses lucilia sericata ovum 0.8 g；In room conditions (temperature 25～30 DEG C, humidity 30～70%), above-mentioned fly blow is cultivated aging to larva, pupate, sprout wings after, feed its adult.
Strain:
S12. the 1st strain: after the Adult worms producting eggs of starting stage, the ovum produced uses the 1st hot housing material to carry out hot housing；Described 1st hot housing material contains each component of following percetage by weight: wheat bran 80%, imported fish meal 20%, with the nicotine aqueous solution of mass percent concentration (1+S) ‰, above-mentioned formulation material being adjusted to humidity is 60%, and S is selected from 0.1～0.5；In room conditions (temperature 25～30 DEG C, humidity 30～70%), above-mentioned fly blow is cultivated aging to larva, pupate, sprout wings after, feed its adult.
S13. the N+1 time strain: the ovum of the adult product of n-th strain gained is carried out hot housing again, and component contained by the compost used by the N+1 time strain meets following ratio: the mass percent concentration of nicotine aqueous solution=(n-th cultivates the content+S of nicotine in compost) ‰, remaining constituent content is constant, N is the natural number more than or equal to 1, repeat strengthening, till the mass percent concentration of nicotine aqueous solution reaches 3 ‰ in compost described in last strain.
Preferably, in this enforcement, in whole breeding phase, the mass percent concentration of nicotine aqueous solution is followed successively by 1.1 ‰, 1.2 ‰, 1.3 ‰, 1.4 ‰, 1.5 ‰, 1.6 ‰, 1.7 ‰, 1.8 ‰, 1.9 ‰, 2.0 ‰, 2.1 ‰, 2.2 ‰, 2.3 ‰, 2.4 ‰, 2.5 ‰, 2.6 ‰, 2.7 ‰, 2.8 ‰, 2.9 ‰, 3.0 ‰；Namely lucilia sericata ovum experiences 20 hot housing altogether.The lucilia sericata kind that this stage turns out i.e. has higher tolerance to nicotine.
Embodiment 5 The lucilia sericata of the resistance to nicotine kind screened is carried out the formulation material of hot housing make it tolerate nicotine aqueous solution that mass percent concentration is 3 ‰
The lucilia sericata of the resistance to nicotine kind that embodiment 1～3 any embodiment screens is cultivated so that it is tolerance mass percent concentration is the formulation material of the nicotine aqueous solution of 3 ‰, and concrete grammar is as follows:
S11. the starting stage:
Preparation initial incubation material, described initial incubation material is made up of each component of following percetage by weight: wheat bran 80%, imported fish meal 20%, and with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 60%.By the above-mentioned compost modulated, every 1 kg loads (long * width * height=45cm*30cm*12cm) in a square plastic feed basin, and every basin accesses lucilia sericata ovum 0.8 g；In room conditions (temperature 25～30 DEG C, humidity 30～70%), above-mentioned fly blow is cultivated aging to larva, pupate, sprout wings after, feed its adult.
Strain:
S12. the 1st strain: after the Adult worms producting eggs of starting stage, the ovum produced uses the 1st hot housing material to carry out hot housing；Described 1st hot housing material contains each component of following percetage by weight: wheat bran 80%, imported fish meal 20%, with the nicotine aqueous solution of mass percent concentration (1+S) ‰, above-mentioned formulation material being adjusted to humidity is 60%, and S is selected from 0.1～0.5；In room conditions (temperature 25～30 DEG C, humidity 30～70%), above-mentioned fly blow is cultivated aging to larva, pupate, sprout wings after, feed its adult；
S13. the N+1 time strain: the ovum of the adult product of n-th strain gained is carried out hot housing again, and component contained by the compost used by the N+1 time strain meets following ratio: the mass percent concentration of nicotine aqueous solution=(n-th cultivates the content+S of nicotine in compost) ‰, remaining constituent content is constant, N is the natural number more than or equal to 1, repeat strengthening, till the mass percent concentration of nicotine aqueous solution reaches 3 ‰ in compost described in last strain.
Preferably, in this enforcement, in whole breeding phase, the mass percent concentration of nicotine aqueous solution is followed successively by 1.2 ‰, 1.4 ‰, 1.6 ‰, 1.8 ‰, 2.0 ‰, 2.2 ‰, 2.4 ‰, 2.6 ‰, 2.8 ‰, 3.0 ‰；Namely lucilia sericata ovum experiences 10 hot housing altogether.The lucilia sericata kind that this stage turns out i.e. has higher tolerance to nicotine.
Embodiment 6 The lucilia sericata of the resistance to nicotine kind screened is carried out the formulation material of hot housing make it tolerate nicotine aqueous solution that mass percent concentration is 3 ‰
The lucilia sericata of the resistance to nicotine kind that embodiment 1～3 any embodiment screens is cultivated so that it is tolerance mass percent concentration is the formulation material of the nicotine aqueous solution of 3 ‰, and concrete grammar is as follows:
S11. the starting stage:
Preparation initial incubation material, described initial incubation material is made up of each component of following percetage by weight: wheat bran 70%, imported fish meal 30%, and with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 60%.By the above-mentioned compost modulated, every 1 kg loads (long * width * height=45cm*30cm*12cm) in a square plastic feed basin, and every basin accesses lucilia sericata ovum 0.8 g；In room conditions (temperature 25～30 DEG C, humidity 30～70%), above-mentioned fly blow is cultivated aging to larva, pupate, sprout wings after, feed its adult.
Strain:
S12. the 1st strain: after the Adult worms producting eggs of starting stage, the ovum produced uses the 1st hot housing material to carry out hot housing；Described 1st hot housing material contains each component of following percetage by weight: wheat bran 80%, imported fish meal 20%, with the nicotine aqueous solution of mass percent concentration (1+S) ‰, above-mentioned formulation material being adjusted to humidity is 60%, and S is selected from 0.1～0.5；In room conditions (temperature 25～30 DEG C, humidity 30～70%), above-mentioned fly blow is cultivated aging to larva, pupate, sprout wings after, feed its adult；
S13. the N+1 time strain: the ovum of the adult product of n-th strain gained is carried out hot housing again, and component contained by the compost used by the N+1 time strain meets following ratio: the mass percent concentration of nicotine aqueous solution=(n-th cultivates the content+S of nicotine in compost) ‰, remaining constituent content is constant, N is the natural number more than or equal to 1, repeat strengthening, till the mass percent concentration of nicotine aqueous solution reaches 3 ‰ in compost described in last strain.
Preferably, in this enforcement, in whole breeding phase, the mass percent concentration of nicotine aqueous solution is followed successively by 1.5 ‰, 2.0 ‰, 2.5 ‰, 3.0 ‰；Namely lucilia sericata ovum experiences 4 hot housing altogether.The lucilia sericata kind that this stage turns out i.e. has higher tolerance to nicotine.
Comparative example
1
Operation with embodiment 1, except for the difference that described in lure collection compost be made up of each component of following percetage by weight: wheat bran 95%, imported fish meal 5%, with the nicotine aqueous solution of mass percent concentration 1 ‰, above-mentioned formulation material being adjusted to humidity is 60%.Experiment finds to utilize and as above lures collection compost almost to lure collection to come to lay eggs less than lucilia sericata kind.
Comparative example
2
Operation with embodiment 1, except for the difference that described in lure collection compost be made up of each component of following percetage by weight: wheat bran 80% and imported fish meal 20%.Experiment find to utilize as above lure collection compost lure collect to ovum be not the most the kind of resistance to nicotine.
Embodiment
7
The lucilia sericata kind that the embodiment of the present invention 1 to 3 screened, embodiment 4 to 6 carry out resistance to nicotine aptitude tests through the lucilia sericata kind of nicotine formulation material of further hot housing tolerance 3 ‰, the lucilia sericata kind after comparative example 1 processes, and test process is:
Take the ovum 0.5g that the lucilia sericata adult after described embodiment cultivation processes is produced respectively, it is respectively connected to the formulation material containing better quality percent concentration nicotine aqueous solution (wheat bran 80%, the imported fish meal 20% of 1kg weight, with the nicotine aqueous solution of mass percent concentration 3.0 ‰, above-mentioned formulation material being adjusted to humidity is 60%) in, test result is shown in Table 1:
Table 1
| Process | Larvae development go through the phase (my god) | Larva bar number/g(bar) | Survival rate of larvae (%) |
| Embodiment 1 is initially planted | 7 | 51 | 60 |
| Embodiment 2 is initially planted | 7 | 50 | 65 |
| Embodiment 3 is initially planted | 7 | 53 | 63 |
| Comparative example 1 | —— | —— | —— |
| Comparative example 2 | 8 | 76 | 13 |
| Embodiment semi-finals kind | 5 | 29 | 96 |
| Embodiment 5 strengthening kind | 5 | 31 | 94 |
| Embodiment 6 strengthening kind | 5 | 33 | 93 |
Test result illustrates, comparative example 1,2 lucilia sericata kind is not resistant to the nicotine aqueous solution of mass percent concentration 3.0 ‰.
Embodiment 1 to 3 there occurs diapause phenomenon through the lucilia sericata kind of screening in the formulation material of the nicotine aqueous solution modulation of mass percent concentration 3.0 ‰, and larval weight is relatively light, and has relatively large death.After embodiment 4 to 6 screening, the resistance to nicotine ability of lucilia sericata kind after the inventive method hot housing processes is greatly improved again, can tolerate the formulation material of the nicotine aqueous solution modulation of mass percent concentration 3.0 ‰.
Claims (6)
1. the intensifying method of the lucilia sericata of a resistance to nicotine kind, it is characterised in that comprise the steps:
Starting stage: the ovum of the lucilia sericata of resistance to nicotine kind is seeded in initial incubation material, described initial incubation
Material is made up of the component of following weight fraction: wheat bran 70～90%, imported fish meal 10～30%, use quality
It is 60% that above-mentioned initial incubation material is adjusted to humidity by the nicotine aqueous solution of percent concentration 1 ‰；In room conditions,
Above-mentioned ovum is cultivated aging to larva, pupate, sprout wings after, feed its adult；
1st strain: after the Adult worms producting eggs of starting stage, the ovum produced uses the 1st strengthening training
Nutriment carries out hot housing；Described 1st hot housing material is made up of each component of following weight fraction: bran
Skin 70～90%, imported fish meal 10～30%, with the nicotine aqueous solution of mass percent concentration (1+S) ‰ by upper
Stating hot housing material and being adjusted to humidity is 60%, and S is selected from 0.1～0.5；In room conditions, above-mentioned ovum is cultivated
Aging to larva, pupate, sprout wings after, feed its adult；
The N+1 time strain: the ovum of the adult product of n-th strain gained is carried out strengthening training again
Support, and component contained by the compost used by the N+1 time strain meets following ratio: nicotine aqueous solution
Mass percent concentration=(content+S of nicotine in n-th hot housing material) ‰, remaining constituent content is not
Becoming, N is the natural number more than or equal to 1, repeats strengthening, until in compost described in last strain
Till the mass percent concentration of nicotine aqueous solution reaches 3 ‰；
The screening technique of the described lucilia sericata of resistance to nicotine kind is:
S1. configuration lures collection compost, and collection compost will be lured to load in open containers；
Described lure collection compost to be made up of each component of following weight fraction: wheat bran 70～90%, imported fish meal
10～30%, lure collection compost to be adjusted to humidity with the nicotine aqueous solution of mass percent concentration 1 ‰～2 ‰ by above-mentioned
It is 60%；
S2., when weather is fine, will be equipped with the morning luring the container of collection compost to be put in house refuse heap, chicken house respectively
Lure the wild fly blow of collection with pig farm, regain the container being put in everywhere afternoon；
S3. each container reclaimed is placed on indoor cultivation, wait lure the wild fly blow that arrives of collection cultivate to larva aging time,
Utilize being separated by fly larvae from food phase behavior of lucilia sericata maggot with compost；
S4. by the Morphological Identification of lucilia sericata larvae, lucilia sericata maggot is chosen；
S5. filter out that 4～5 days aging, every fly larvae weighs 0.03～0.05g, figure is plump, color is golden yellow
Lucilia sericata maggot, at ambient temperature, allow lucilia sericata maggot naturally pupate, sprout wings after can obtain resistance to nicotine
Lucilia sericata kind.
Intensifying method the most according to claim 1, it is characterised in that described indoor conditions be temperature 25～
30 DEG C, humidity 30～70%.
Intensifying method the most according to claim 1, it is characterised in that the compound method of described initial incubation material
After each component of initial incubation material being mixed, the humidity of compost is adjusted by the nicotine aqueous solution adding certain concentration
To 60%.
Intensifying method the most according to claim 1, it is characterised in that access silk in every 1kg initial incubation material
Light cryptolucilia caesarion (greenbottle fly) ovum 0.5～1.0g；Every 1kg hot housing material accesses lucilia sericata ovum 0.5～1.0g.
Intensifying method the most according to claim 1, it is characterised in that its adult of described nursing is all to utilize entirely
Fat milk powder, brown sugar and water are fed.
Intensifying method the most according to claim 5, it is characterised in that according to percent by weight, described entirely
Fat milk powder, the usage ratio of brown sugar are whole milk powder 70%, brown sugar 30%；The mode that water is fed is to utilize sea
Silk floss is saturated with water, sucks for adult.
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| CN103975901A (en) * | 2014-05-30 | 2014-08-13 | 中山大学 | Method for trapping and collecting wild hermetia illucens by utilizing chrysomyia megacephala maggot cultivation residues |
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| CN101889629A (en) * | 2010-06-13 | 2010-11-24 | 中山大学 | Method for processing food waste by using black soldier fly larvae and material formula |
| CN102150644A (en) * | 2010-12-23 | 2011-08-17 | 浙江千年春生物技术有限公司 | Breeding method for antitumor chrysomyia megacephala larvae |
| CN103283681A (en) * | 2012-03-05 | 2013-09-11 | 朱大恒 | Biological utilization method of waste tobacco stems |
| CN103651263A (en) * | 2013-11-07 | 2014-03-26 | 中山大学 | Cultivation method of nicotine-resistant housefly strain |
| CN103975901A (en) * | 2014-05-30 | 2014-08-13 | 中山大学 | Method for trapping and collecting wild hermetia illucens by utilizing chrysomyia megacephala maggot cultivation residues |
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