CN104004801B - 一种制备单唾液酸四己糖神经节苷脂的方法 - Google Patents
一种制备单唾液酸四己糖神经节苷脂的方法 Download PDFInfo
- Publication number
- CN104004801B CN104004801B CN201410254826.0A CN201410254826A CN104004801B CN 104004801 B CN104004801 B CN 104004801B CN 201410254826 A CN201410254826 A CN 201410254826A CN 104004801 B CN104004801 B CN 104004801B
- Authority
- CN
- China
- Prior art keywords
- monostalotetrahexosylgangliside
- protein
- ccesia
- recombinant vector
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000000034 method Methods 0.000 title claims abstract description 47
- 108090000790 Enzymes Proteins 0.000 claims abstract description 130
- 102000004190 Enzymes Human genes 0.000 claims abstract description 130
- SQVRNKJHWKZAKO-UHFFFAOYSA-N beta-N-Acetyl-D-neuraminic acid Natural products CC(=O)NC1C(O)CC(O)(C(O)=O)OC1C(O)C(O)CO SQVRNKJHWKZAKO-UHFFFAOYSA-N 0.000 claims abstract description 83
- 230000003301 hydrolyzing effect Effects 0.000 claims abstract description 83
- SQVRNKJHWKZAKO-OQPLDHBCSA-N sialic acid Chemical compound CC(=O)N[C@@H]1[C@@H](O)C[C@@](O)(C(O)=O)OC1[C@H](O)[C@H](O)CO SQVRNKJHWKZAKO-OQPLDHBCSA-N 0.000 claims abstract description 82
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 60
- 150000002270 gangliosides Chemical class 0.000 claims abstract description 50
- 235000018102 proteins Nutrition 0.000 claims abstract description 45
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 45
- 239000000758 substrate Substances 0.000 claims abstract description 17
- 125000003275 alpha amino acid group Chemical group 0.000 claims abstract description 15
- 238000006555 catalytic reaction Methods 0.000 claims abstract description 15
- 239000000376 reactant Substances 0.000 claims abstract description 7
- 239000013598 vector Substances 0.000 claims description 34
- 238000000746 purification Methods 0.000 claims description 26
- 108020004707 nucleic acids Proteins 0.000 claims description 24
- 102000039446 nucleic acids Human genes 0.000 claims description 24
- 150000007523 nucleic acids Chemical class 0.000 claims description 24
- 239000012620 biological material Substances 0.000 claims description 22
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 21
- 239000007788 liquid Substances 0.000 claims description 21
- 210000004027 cell Anatomy 0.000 claims description 17
- 244000005700 microbiome Species 0.000 claims description 16
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims description 14
- 210000004102 animal cell Anatomy 0.000 claims description 14
- 239000006228 supernatant Substances 0.000 claims description 13
- 230000009261 transgenic effect Effects 0.000 claims description 13
- 239000000203 mixture Substances 0.000 claims description 7
- 239000012535 impurity Substances 0.000 claims description 4
- 238000010898 silica gel chromatography Methods 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 21
- 125000000539 amino acid group Chemical group 0.000 abstract description 7
- 230000008034 disappearance Effects 0.000 abstract description 3
- 238000002474 experimental method Methods 0.000 abstract description 2
- 241000894006 Bacteria Species 0.000 description 45
- 230000006698 induction Effects 0.000 description 26
- 239000012460 protein solution Substances 0.000 description 21
- 239000002773 nucleotide Substances 0.000 description 20
- 125000003729 nucleotide group Chemical group 0.000 description 20
- 238000006243 chemical reaction Methods 0.000 description 18
- 239000002253 acid Substances 0.000 description 17
- 108020004414 DNA Proteins 0.000 description 15
- 239000000047 product Substances 0.000 description 15
- -1 ganglioside GLS Chemical class 0.000 description 13
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 12
- 239000000835 fiber Substances 0.000 description 11
- 230000009182 swimming Effects 0.000 description 11
- 239000000243 solution Substances 0.000 description 10
- 239000003153 chemical reaction reagent Substances 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- 241000282894 Sus scrofa domesticus Species 0.000 description 7
- 239000002609 medium Substances 0.000 description 7
- 229940060155 neuac Drugs 0.000 description 7
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 6
- RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 description 6
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 5
- 239000002299 complementary DNA Substances 0.000 description 5
- 238000001514 detection method Methods 0.000 description 5
- 150000002460 imidazoles Chemical class 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 229910021645 metal ion Inorganic materials 0.000 description 5
- 238000005903 acid hydrolysis reaction Methods 0.000 description 4
- 230000029087 digestion Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 238000000855 fermentation Methods 0.000 description 4
- 230000004151 fermentation Effects 0.000 description 4
- 239000012634 fragment Substances 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M sodium hydroxide Inorganic materials [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 230000009471 action Effects 0.000 description 3
- 230000004913 activation Effects 0.000 description 3
- 238000000246 agarose gel electrophoresis Methods 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 239000000499 gel Substances 0.000 description 3
- 229930027917 kanamycin Natural products 0.000 description 3
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 3
- 229960000318 kanamycin Drugs 0.000 description 3
- 229930182823 kanamycin A Natural products 0.000 description 3
- 230000035772 mutation Effects 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 108020004705 Codon Proteins 0.000 description 2
- 102000053602 DNA Human genes 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 241001198387 Escherichia coli BL21(DE3) Species 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 2
- 229910019142 PO4 Inorganic materials 0.000 description 2
- 108010076504 Protein Sorting Signals Proteins 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 230000009849 deactivation Effects 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 239000013604 expression vector Substances 0.000 description 2
- 238000004108 freeze drying Methods 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 2
- 239000010452 phosphate Substances 0.000 description 2
- 239000013612 plasmid Substances 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 238000003259 recombinant expression Methods 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 238000002390 rotary evaporation Methods 0.000 description 2
- 230000003248 secreting effect Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 125000005629 sialic acid group Chemical group 0.000 description 2
- 229910000162 sodium phosphate Inorganic materials 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 210000004243 sweat Anatomy 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- 208000024827 Alzheimer disease Diseases 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 102000012410 DNA Ligases Human genes 0.000 description 1
- 108010061982 DNA Ligases Proteins 0.000 description 1
- 238000007400 DNA extraction Methods 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 108700028146 Genetic Enhancer Elements Proteins 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 239000006142 Luria-Bertani Agar Substances 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 208000028389 Nerve injury Diseases 0.000 description 1
- 208000012902 Nervous system disease Diseases 0.000 description 1
- 241001597008 Nomeidae Species 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 208000018737 Parkinson disease Diseases 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 241000282898 Sus scrofa Species 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical group 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000036983 biotransformation Effects 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 210000005013 brain tissue Anatomy 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 230000004087 circulation Effects 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000010812 external standard method Methods 0.000 description 1
- 239000006052 feed supplement Substances 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- QPJBWNIQKHGLAU-IQZHVAEDSA-N ganglioside GM1 Chemical compound O[C@@H]1[C@@H](O)[C@H](OC[C@H](NC(=O)CCCCCCCCCCCCCCCCC)[C@H](O)\C=C\CCCCCCCCCCCCC)O[C@H](CO)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@]2(O[C@H]([C@H](NC(C)=O)[C@@H](O)C2)[C@H](O)[C@H](O)CO)C(O)=O)[C@@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O3)O)[C@@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](CO)O1 QPJBWNIQKHGLAU-IQZHVAEDSA-N 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 150000001455 metallic ions Chemical class 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- UPSFMJHZUCSEHU-JYGUBCOQSA-N n-[(2s,3r,4r,5s,6r)-2-[(2r,3s,4r,5r,6s)-5-acetamido-4-hydroxy-2-(hydroxymethyl)-6-(4-methyl-2-oxochromen-7-yl)oxyoxan-3-yl]oxy-4,5-dihydroxy-6-(hydroxymethyl)oxan-3-yl]acetamide Chemical compound CC(=O)N[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@H]1[C@H](O)[C@@H](NC(C)=O)[C@H](OC=2C=C3OC(=O)C=C(C)C3=CC=2)O[C@@H]1CO UPSFMJHZUCSEHU-JYGUBCOQSA-N 0.000 description 1
- 230000008764 nerve damage Effects 0.000 description 1
- 230000001537 neural effect Effects 0.000 description 1
- 230000004770 neurodegeneration Effects 0.000 description 1
- 208000015122 neurodegenerative disease Diseases 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
- 108091033319 polynucleotide Proteins 0.000 description 1
- 102000040430 polynucleotide Human genes 0.000 description 1
- 239000002157 polynucleotide Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- QLNJFJADRCOGBJ-UHFFFAOYSA-N propionamide Chemical compound CCC(N)=O QLNJFJADRCOGBJ-UHFFFAOYSA-N 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 238000000611 regression analysis Methods 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- 239000013603 viral vector Substances 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Landscapes
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410254826.0A CN104004801B (zh) | 2014-06-09 | 2014-06-09 | 一种制备单唾液酸四己糖神经节苷脂的方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410254826.0A CN104004801B (zh) | 2014-06-09 | 2014-06-09 | 一种制备单唾液酸四己糖神经节苷脂的方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN104004801A CN104004801A (zh) | 2014-08-27 |
CN104004801B true CN104004801B (zh) | 2016-06-29 |
Family
ID=51365707
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201410254826.0A Active CN104004801B (zh) | 2014-06-09 | 2014-06-09 | 一种制备单唾液酸四己糖神经节苷脂的方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN104004801B (zh) |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101636489A (zh) * | 2007-02-20 | 2010-01-27 | 帝斯曼知识产权资产管理有限公司 | 新颖的唾液酸酶 |
-
2014
- 2014-06-09 CN CN201410254826.0A patent/CN104004801B/zh active Active
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101636489A (zh) * | 2007-02-20 | 2010-01-27 | 帝斯曼知识产权资产管理有限公司 | 新颖的唾液酸酶 |
Non-Patent Citations (3)
Title |
---|
hypothetical protein N880_29145,partial[Bacillus sp.EGD-AK10];Kapley,A.et al.;《GenBank》;20130911;登录号:ERI40651 * |
产唾液酸酶微生物的筛选及产酶条件的优化;曹丹 等;《生物加工过程》;20090731;第7卷(第4期);40-45 * |
固定化唾液酸酶催化多唾液酸神经节苷脂制备 GM1;沈丹虹 等;《中国医药工业杂志》;20121231;第43卷(第10期);821-823,841 * |
Also Published As
Publication number | Publication date |
---|---|
CN104004801A (zh) | 2014-08-27 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN107574159B (zh) | 一种以活性形式表达的谷氨酰胺转氨酶的突变体 | |
Dudkina et al. | Three‐step procedure for preparation of pure Bacillus altitudinis ribonuclease | |
CN110054702B (zh) | 玉米赤霉烯酮降解酶融合蛋白及其编码基因和应用 | |
CN108342378B (zh) | 一种谷氨酸脱羧酶突变体及其编码基因与应用 | |
CN109609475A (zh) | 草铵膦脱氢酶突变体及其合成l-草铵膦的应用 | |
CN102839165A (zh) | 基因突变型重组蛋白酶k及其工业化生产方法 | |
Ersayin Yasinok et al. | Xylanase from a soil isolate, Bacillus pumilus: gene isolation, enzyme production, purification, characterization and one-step separation by aqueous-two-phase system | |
CN101591664A (zh) | 一种高对映体选择性环氧化物水解酶及其编码的基因 | |
CN111770996A (zh) | 突变β-葡萄糖苷酶 | |
CN110592045B (zh) | 一种重组酯酶、基因、工程菌及拆分(r,s)-吲哚啉-2-甲酸乙酯的应用 | |
CN104004801B (zh) | 一种制备单唾液酸四己糖神经节苷脂的方法 | |
CN109355274B (zh) | 一种对胰蛋白酶和胃蛋白酶抗性提高的β-葡萄糖苷酶 | |
CN115058408B (zh) | 一种宏基因组来源的高比活耐酸性d-阿洛酮糖3-差向异构酶及其编码基因和应用 | |
CN114349831B (zh) | aspA基因突变体、重组菌及制备L-缬氨酸的方法 | |
CN104004728B (zh) | 一种具有唾液酸水解酶活性的蛋白质及其应用 | |
CN105420220B (zh) | 一种天冬氨酸类蛋白酶及其编码基因与应用 | |
CN104004727B (zh) | 一种具有唾液酸水解酶活性的蛋白质的制备方法 | |
CN111757939B (zh) | 突变β-葡萄糖苷酶 | |
CN109402084B (zh) | 构建具有溶磷活性重组微生物的方法及其所用核酸分子 | |
CN112481235B (zh) | Trdrs2蛋白及其相关生物材料在调控里氏木霉合成和分泌蛋白能力中的应用 | |
CN104762280B (zh) | 一种重组糖苷水解酶用于生物转化制备人参二醇型皂苷的工艺 | |
CN111484988A (zh) | 一种具有木聚糖酶和阿魏酸酯酶活性的双功能酶及其编码基因和应用 | |
CN103088004A (zh) | 一种重组耐热的β-1,4内切纤维素酶的纯化方法 | |
CN103740749B (zh) | 一种重组表达内切葡聚糖酶的工程菌株 | |
CN107119035A (zh) | 苯丙氨酸变位酶、编码基因、重组载体、宿主细胞、多重pcr引物及它们的应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
ASS | Succession or assignment of patent right |
Owner name: JILIN NORTHEAST NORMAL UNIVERSITY ASSETS MANAGEMEN Free format text: FORMER OWNER: NORTHEAST NORMAL UNIVERSITY Effective date: 20150818 |
|
C41 | Transfer of patent application or patent right or utility model | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20150818 Address after: 130117 Jilin province Changchun Jingyue richfruits Road No. 1138 Applicant after: Jilin Northeast Normal University Assets Management Co., Ltd. Address before: 130024 Jilin City, Changchun province people's street, No. 5268 Applicant before: Northeast Normal University |
|
C41 | Transfer of patent application or patent right or utility model | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20160517 Address after: 130024 Jilin City, Changchun province people's street, No. 5268 Applicant after: Northeast Normal University Address before: 130117 Jilin province Changchun Jingyue richfruits Road No. 1138 Applicant before: Jilin Northeast Normal University Assets Management Co., Ltd. |
|
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
C41 | Transfer of patent application or patent right or utility model | ||
TR01 | Transfer of patent right |
Effective date of registration: 20170118 Address after: 130103 Changchun high tech Industrial Development Zone, Jilin, the extraordinary street, No. 707 Patentee after: Jilin Qijian Biotechnology Co., Ltd. Address before: 130024 Jilin City, Changchun province people's street, No. 5268 Patentee before: Northeast Normal Univ. |
|
TR01 | Transfer of patent right |
Effective date of registration: 20171204 Address after: No. 707, No. 2, superavenue, Jilin high tech Industrial Development Zone, Changchun, Jilin Patentee after: Jilin first Health Biotechnology Co., Ltd. Address before: 130103 Changchun high tech Industrial Development Zone, Jilin, the extraordinary street, No. 707 Patentee before: Qijian Biological Technology Co., Ltd., Jilin |
|
TR01 | Transfer of patent right |